WO2015091189A1 - Réalisation de motifs sélective de membranes de filtration - Google Patents

Réalisation de motifs sélective de membranes de filtration Download PDF

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Publication number
WO2015091189A1
WO2015091189A1 PCT/EP2014/077330 EP2014077330W WO2015091189A1 WO 2015091189 A1 WO2015091189 A1 WO 2015091189A1 EP 2014077330 W EP2014077330 W EP 2014077330W WO 2015091189 A1 WO2015091189 A1 WO 2015091189A1
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WO
WIPO (PCT)
Prior art keywords
web
filter
filtering
pores
base portion
Prior art date
Application number
PCT/EP2014/077330
Other languages
English (en)
Inventor
Godefridus Johannes Verhoeckx
Ron Martinus Laurentius Van Lieshout
Toon Hendrik Evers
Olli-Heikki Huttunen
Ralph Liedert
Inka PAKKILA
Sari TAIPALE
Original Assignee
Koninklijke Philips N.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninklijke Philips N.V. filed Critical Koninklijke Philips N.V.
Publication of WO2015091189A1 publication Critical patent/WO2015091189A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08—Flat membrane modules
    • B01D63/081—Manufacturing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08—Flat membrane modules
    • B01D63/088—Microfluidic devices comprising semi-permeable flat membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0081—After-treatment of organic or inorganic membranes
    • B01D67/0088—Physical treatment with compounds, e.g. swelling, coating or impregnation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/66—Polymers having sulfur in the main chain, with or without nitrogen, oxygen or carbon only
    • B01D71/68—Polysulfones; Polyethersulfones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2323/00—Details relating to membrane preparation
    • B01D2323/04—Hydrophobization
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2323/00—Details relating to membrane preparation
    • B01D2323/08—Specific temperatures applied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation

Definitions

  • the invention relates to the field of filters, filter units/modules or complete microfluidic devices (e.g. disposable cartridges for diagnostic applications) with integrated filtration membranes, which enable roll-to-roll or sheet-based manufacturing.
  • a filter is usually a matrix containing pores through which only a part of the sample can go through and is therefore filtered.
  • Such filters can be used for detection of marker molecules in bodily fluids.
  • An example of a marker molecule is troponin-I (cTnl) used for the detection of cardio-vascular disease.
  • the filter is used to separate the cell fraction (e.g. erythrocytes, leucocytes) from the liquid fraction (plasma), and thereby generate plasma.
  • cell fraction e.g. erythrocytes, leucocytes
  • plasma e.g. erythrocytes, leucocytes
  • Fig. 1 shows a schematic cross section of a blood filter which is built up of a first layer 10 with pores that are large enough to capture and to filter out cells and a second layer 20 with small pores that can not be penetrated by cells to facilitate transportation of the plasma. Due to the large amount of cells the filter needs to have a large volume to prevent clogging of the filter. This leads to a large dead volume inside the filter, also on the small- pore side and increases the contact time of the plasma with the filter. Due to the small pores in the plasma transport region, the internal surface area of the filter is relatively large.
  • roll-to-roll processing also known as web processing, reel-to-reel processing or R2R
  • R2R reel-to-reel processing
  • Some R2R processes of manufacturing filters are known, e.g. from US 6,803,019. However, these processes require many steps of manufacturing.
  • manufacturing micro fluidic filter units for filtering a sample e.g.
  • the described product concepts prevent handling (positioning, re-orientation) of individual fragile pieces of filter membrane by e.g. pick and place techniques.
  • a further object of the present invention is to provide a method of manufacturing filter units or filters with improved handling options.
  • filter or filter units can be patterned by selectively inactivating or inhibiting the filtering function of the filter material, by collapsing and/or clogging the pores, e.g. by using an adapted solvent or chemical compound.
  • This allows a patterning of the filter device, avoiding dead volumes or functionalizing the filter device.
  • the contact area of the filter device can be reduced by selective patterning. Thereby, a large part of the area of the filter device is reduced while the ability to filter out a large amount of cells is kept.
  • the "effective" filtration area of the membrane can be clearly defined, thereby preventing lateral flow of sample or filtrate into regions of the filter which are not required for the filtering or transport function and the filter can be optimized for maximum filtration speed (related to the "top” volume) and minimal retention (related to the "bottom” internal surface area and dead volume (related to the bottom internal volume).
  • a chemical component may be arranged on the predetermined zones to collapse the pores. Thereby, a quick and efficient inhibition (at least a part thereof) of the filtering function can be achieved.
  • the chemical compound may be a solvent, specifically tetrahydroiuran
  • the filter material may be a polysulfone-based membrane. This provides the advantage of rapid dissolving and evaporation so that the solvent has no time to spread over a wider area.
  • the web portion may comprise a first layer with larger pores (e.g. for preventing clogging) and a second layer with smaller pores (e.g. for generating or filtering plasma from blood).
  • the chemical compound may be arranged so as to pattern the first layer and the second layer or the second layer only.
  • the total internal pore area of the filter device can be reduced.
  • the side with the larger pores is kept open to prevent clogging of the filter device.
  • the filter device may comprise a foil or cover arranged on top of the web portion opposite to the base portion and comprising an aperture extending over at least a part of the filtering active area.
  • the applied sample can be directed via the aperture to the filtering active area.
  • the invention proposes a method of manufacturing filter units for filtering samples and collecting the filtered samples, the method comprising the steps of:
  • this method further comprises the steps of:
  • said inhibition step can be implemented by different methods, such as:
  • hydrophobization fluids comprise for example: siloxanes (e.g. octadecyltrichlorosilane); oleaginous materials; (mineral) oil; Zonyl 8857A (DuPont, USA); DURALON UltraTec (COTEC GmbH, Karlstein, Germany); AF1600, AF2400 Fluoropolymers (DuPont); EGC-1700 (3M, USA); Halar® ECTFE (Solvay Solexis); Tefzel® ETFE (DuPont); or Teflon® FEP (DuPont) (wherein a selection has to be made in relation to assay interference of these chemicals).
  • siloxanes e.g. octadecyltrichlorosilane
  • oleaginous materials e.g. octadecyltrichlorosilane
  • oleaginous materials e.g. octadecyltrichlorosilane
  • steps (b2) and (c2), or steps (b3) and (c3), or steps (b) and (c) of said manufacturing method may be implemented simultaneously using a continuous web.
  • an adhesive layer may be applied between the first base portion and the first portion of the web and/or an adhesive layer may be applied between the second base and the second portion of the web, the adhesive layer being provided with a through hole facing, respectively, the first and second filtering active area.
  • the adhesive layer may act as a lid to close micro fluidic channels of the micro fluidic element.
  • the top foil or cover and the adhesive layer may be provided by using the same layer which has been folded to cover the web and to be sandwiched between the web and the base portion. This allows efficient manufacturing of both layers.
  • a cut-out step may be provided to cut out the web so as to separate the first and the second filter units. Again a measure which allows efficient roll-to-roll manufacturing of filter modules or complete filter devices with integrated filter function.
  • said first and the second substance may be applied by ink jet or micro droplet deposition. This measure allows accurate patterning by a controlled collapse of the porous structure of the web.
  • Fig. 1 shows schematic cross section of a two-layered blood filter
  • Fig. 2 shows a schematic top view of blood filter with a ring-shaped blocking area according to a first embodiment
  • Fig. 3 shows a photographs of a portion of a filter processed according to the invention
  • Fig. 4 shows a diagram indicating the signal response per unit of concentration of target (cTnl) for a conventional filter (left bar “F”) and the filter with reduced filter area according to first embodiment (right bar “THF-F);
  • Fig. 5 shows two further examples of patterned filters according to second and third embodiments
  • Fig. 6 shows a flow diagram of a manufacturing process according to a fifth embodiment
  • Figs. 7a to 7i show cross sections of a diagnostic device or cartridge with integrated blood filtration function during different assembling steps.
  • THF tetrahydrofuran
  • PES polysulfone
  • the area of the filter is reduced while keeping the filter capacity and preventing clogging due to large amount of cells.
  • the filter may be patterned only on the side where the plasma is transported through the filter in order to only pattern the side with the small pores.
  • the patterning is done by dosing of the above solvent for the filter material. Dissolution of the filter material causes the pores to collapse and become impermeable for the plasma. Patterning of only the small pores can be achieved by carefully balancing the dosed amount and using the strong capillary pull of the area with smaller pores to localize the patterning liquid.
  • the "effective" filter size of the membrane can be clearly defined and optimized for speed at the required amount of sample and the internal area can be reduced for increased plasma recovery and lower analyte retention. More specifically, by patterning the filter with the solvent the filter material is dissolved. When the solution evaporates, the filter material hardens again into a solid piece and thus blocks the flow of liquid.
  • the solvent can be deposited by ink jet and micro droplet onto the porous polysulfone blood filtration membrane.
  • a solvent which dissolves PES very rapidly such as THF.
  • THF is a very aggressive solvent to PES based filtration membranes and dissolves it rapidly.
  • THF effectively causes the collapse of the pores within the membrane and clogs all fluidic pathways which could transfer any liquid and especially plasma through the membrane.
  • the quick evaporation ensures that the solvent has no time to spread within the pores of the filter membrane over a wider area. If a less aggressive solvent is used (e.g. toluene), the solvent has time to spread all over the membrane before it evaporates and no accurate patterning can be achieved.
  • a less aggressive solvent e.g. toluene
  • a typical method to assemble blood filtration membrane patches onto diagnostic test strips is by pick-and-place technology.
  • This requires filter membrane patches which are aligned and/or pre-sorted on e.g. a material feeding belt or box.
  • the problem with pick-and-place handling of filtration patches is that the orientation of the filter membrane also has to be considered.
  • the top side of the filter membrane may contain larger pores and the bottom side of the filter membrane may contain finer pores, so that wrong orientation of the membrane compromises the functionality of the filtration patch. This issue is solved automatically when the filter region is part of a continuous web.
  • a polysulfone (PES) membrane is an extremely brittle material and difficult to handle with pick-and-place equipment.
  • PES raw material is delivered as a roll stock
  • patterning of the filter membrane with a printing method e.g. THF ink
  • Fig. 2 shows a schematic top view of a filter according to a first embodiment, which is patterned on both sides (i.e. large and small porous layer) to prevent blood from spreading.
  • a THF solution is deposited on top of the filter, i.e., on the first layer 10 with the larger pores, to thereby create a ring-shaped area 40 where both the large and the small pores area of the filter are blocked, so that the filter area surrounded by the ring-shaped area 40 is confined.
  • Inkjet printing or micro droplet deposition of THF onto a PES-based blood filtration membrane enables accurate patterning of an "effective" filtration area by a controlled collapse of the porous structure of the filtration membrane on the borders of the filtration area. In this way, the confined total internal pore area of the filter is reduced and analyte retention is reduced.
  • Fig. 3 shows a sectional top view of an exemplary filter membrane with the first layer 10 of larger pore width patterned with 100% THF by inkjet printing.
  • the applied THF ink collapses the filter membrane 10 sufficiently to block liquid flow across the barrier pattern.
  • Three ring-shaped areas 40 i.e. barrier rings with line width: e.g. 1mm; distance between rings: e.g. 1mm) were printed to provide additional barrier rings in case of leakage through one of the inner rings. However, one barrier ring can be sufficient already.
  • collapsed pores are shown in comparison to the remaining pores of the non-patterned areas of the first layer 10.
  • a plurality of patterned filters as shown in Fig. 2 or 3 can be arranged in a row or matrix on a continuous web to allow R2R or sheet-based manufacturing.
  • Fig. 4 shows a diagram indicating signal response for a conventional filter (left bars “F” in Fig. 4) and the filter with reduced filter area according to first embodiment (right bars “THF-F” in Fig. 4).
  • the signal response is increased when reducing the filter area with THF.
  • the increase of the signal change has the same factor as the decrease of area of the filter.
  • the filter is brought into contact for 5 min with spiked plasma by totally submerging it into a certain volume. After incubating it, the signal response is increased (higher protein concentration or less degradation) with the same ratio as the decrease of the area of the filter, which indicates that the retention and/or sheering of the protein (marker) is less in the patterned filter.
  • the patterning was obtained throughout the whole filter, i.e. through both layers of Fig. 1.
  • the area of the first layer on top of the filter needs to be as large as possible to prevent any clogging of the filter with cells.
  • the patterning liquid e.g. THF
  • the patterning liquid is only transported throughout the second layer with the smaller pores by low volume and the difference in capillary forces, so that the side of the first layer with the larger pores can be kept open.
  • Fig. 5 shows bottom views and cross-sectional views of patterned filters with other specific patterns, where only the side of the second layer 20 with the small pores is blocked in order to facilitate plasma flow and reduce the surface area of the filter. Spaces are left un-patterned to facilitate transport of plasma.
  • a third embodiment is shown in the upper portion of Fig. 5, where the first layer 10 is not patterned and the second layer 20 is patterned into radial lines separated by segments of THF deposition areas 40.
  • a fourth embodiment is shown in the lower portion of Fig. 5, where the first layer 10 is not patterned and the second layer 20 is patterned by circular segments of THF deposition areas 40.
  • the area of the second layer 20 with smaller pores has a high internal surface area, the blocking of this area decreases binding/absorption of biomarkers to the material to a large extent.
  • the filter patterns shown in Fig. 5 can be surrounded by a ring-shaped blocking region, which is a collapsed area for the small- and large-pore side, to confine the sample.
  • a plurality of the patterned filters shown in Fig. 5 can be arranged in a row or matrix on a continuous web to allow R2R or sheet-based manufacturing.
  • the filter or filter units according to the above embodiments are especially suitable for R2R manufacturing.
  • This manufacturing method relies on the fact that the filter membrane is part of a continuous web of the same material and is separated from that web after it has been assembled to other parts (foils or tapes) of the filter, filter unit or cartridge.
  • the proper functioning of such R2R manufacturing relies on the fact that the filter membrane is partly blocked on both sides, thus preventing lateral flow of plasma or sample into regions of the membrane that are not effective in delivery of sample to the rest of the device.
  • This blocking can be achieved as described in the above embodiments.
  • Fig. 6 shows an exemplary process flow for manufacturing of a blood filtration module according to the above embodiments and Figs. 7a to 7i show respective cross sections of the blood filtration module during the different assembling steps of the process flow.
  • a transfer tape 60 with release liner 50, 52 on both sides e.g. 15 g/m 2 pure acrylic adhesive
  • Fig. 7a a transfer tape 60 with release liner 50, 52 on both sides (e.g. 15 g/m 2 pure acrylic adhesive) is provided, as shown in Fig. 7a.
  • step S502 circular holes and a perforate middle line are generated by a respective die-cutting tool 70 or a laser (not shown), as shown in Fig. 7b.
  • the perforate middle line is not a perforate in the sense that the cutting is partial over the length of the line (dashing).
  • step S503 the upper release liner 50 is removed, as shown in Fig. 7c.
  • step S504 a top cover laminate 80 (left) and a base part 90 which may contain microfluidic and other functions 92, such as supporting detection of the analyte (right) are added, as shown in Fig. 7d.
  • the base part 90 could be a single part or a subassembly, containing several functions, if the end product is a complete cartridge with integrated filter.
  • step S505 the assembly is flipped around, as shown in Fig. 7e.
  • step S506 the remaining lower release liner 52 is removed, as shown in Fig. 7f.
  • a blood filtration membrane 100 is added on top of the flipped part 90 and aligned with the part 90, as shown in Fig. 7g.
  • step S508 the blood filtration membrane is patterned with THF solvent 120 by an ink jet printing or flexography mechanism 110 to obtain solvent deposition areas (or non- filtering zones) 130, as shown in Fig. 7h. This solvent selectively collapses pores of the filtration membrane 100, inhibiting locally (in zones 130) at least partly its filtering function.
  • step S509 the obtained sheet or web is diced and folded into individual cartridges, as shown in Fig. 7i.
  • predetermined zones 130 or other zones can be inhibited by other ways, such as for example the local filling up of the pores of the filtration membrane 100 in said predetermined zones with a fluid or solid filling material and/or by making that region very hydrophobic by the application or generation of a hydrophobic filling material to the pores of the filter material.
  • a fluid or solid filling material such as for example the local filling up of the pores of the filtration membrane 100 in said predetermined zones with a fluid or solid filling material and/or by making that region very hydrophobic by the application or generation of a hydrophobic filling material to the pores of the filter material.
  • Suited hydrophobization fluids comprise for example: siloxanes (e.g. octadecyltrichlorosilane); oleaginous materials; (mineral) oil; Zonyl 8857A (DuPont, USA); DURALON UltraTec (COTEC GmbH, Karlstein, Germany); AF1600, AF2400 Fluoropolymers (DuPont); EGC-1700 (3M, USA); Halar® ECTFE (Solvay Solexis); Tefzel® ETFE (DuPont); or Teflon® FEP (DuPont) (wherein a selection has to be made in relation to assay interference of these chemicals).
  • siloxanes e.g. octadecyltrichlorosilane
  • oleaginous materials e.g. octadecyltrichlorosilane
  • oleaginous materials e.g. octadecyltrichlorosi
  • laminate patterning may be achieved by knife plotter cutting, where a knife plotter is used in sheet process, whereas die-cutting or a laser can be used in the roll-to-roll process.
  • a hydrophobic spacer tape may be used for sheet assembly of e.g. five blood filtration modules simultaneously.
  • the laminate sheet may be fixed on the assembly table by a tape with the protective top liner 50 removed.
  • step S509 the top cover is folded over (from left to right) on top of the base part 90.
  • the glue contact may be increased by rolling over the filter stack with a silicone roller.
  • the filter cartridges 90 can now be diced into individual devices (achieved here by the perforation cuts on the device borders which are already added in step S502).
  • the base part 90 could represent a complete base part with microfiuidic and detection functions on board.
  • the product sketched in Fig 7i then represents a complete disposable cartridge or diagnostic device with an integrated filter function.
  • a filter module (subassembly to be mounted to any device) can be made by keeping part of the top liner 50 and removing that just before mounting.
  • filter units or modules can be manufactured, which can be assembled onto any device or cartridge. In this case, only the left part of the top liner 50 would be removed in step S503, enabled by "kiss cutting" through the top liner 50.
  • filter units for filtering samples and collecting the filtered samples can be manufactured by providing one continuous web made of a filter material (i.e.
  • filtration membrane 100 and then obtaining a first filter unit by providing a first (rigid) base or base portion comprising a first microfiuidic element to collect some filtered sample, then applying a first portion of the web onto the first (rigid) base or base portion, and then applying a substance onto predetermined zones of the first portion of the web so as to define a first filtering active area covering at least a part of the first microfiuidic element.
  • a second filter unit can be obtained by providing a second (rigid) base or base portion comprising a second microfiuidic element to collect some filtered sample, then applying a second portion of the web onto the second (rigid) base or base portion, and then applying a substance onto predetermined zones of the second portion of the web so as to define a second filtering active area covering at least a part of the second microfiuidic element.
  • any substance e.g. THF
  • the application of the web portions or the substances for the first and second filters or even the complete assembly of the two filters can be done simultaneously.
  • application of the substance for the first filter could be implemented after or before the application of the web portion for the first filter
  • application of the substance for the second filter could be implemented after or before the application of the web portion for the second filter.
  • the web tends to warp (due to the liquid solvents deposited onto the filtering membrane) when it is not attached to a solid base. In a mass-manufacturing environment this might lead to web shrinkage and to downstream alignment problems. Therefore, the web should be exposed to the solvent after it has been attached to a rigid base.
  • an adhesive layer can be applied between the first (rigid) base and the first portion of the web and/or between the second (rigid) base and the second portion of the web.
  • the adhesive layer may be provided with a through hole facing, respectively, the first and second filtering active area.
  • this adhesive layer can act as a lid to close the microfluidic channels comprised in the microfluidic elements.
  • this adhesive layer is one or a combination of the following: acrylic adhesive film sandwiched between the web and the bases or a printed layer of adhesive material directly deposited onto the web.
  • a top foil or cover may be applied onto the surface of the web opposite to the surface of the web applied to the (rigid) bases.
  • the foil or cover comprises a first aperture and a second aperture for covering, respectively, at least a part of the first filtering active area and at least a part of the second filtering area.
  • the top foil or cover and the adhesive layer can be made of the same layer which has been folded to cover the web (and acts as a top foil or cover) and to be sandwiched between the web and the base (and acts as an adhesive layer).
  • a cut-out step may be provided to cut out the web so as to separate the first and the second filter units (and possibly the foil or cover if any)
  • the above first and second (rigid) bases may be two portions of a single (rigid) board, and a cut-out step may be provided for cutting out the assembly of the first (rigid) base and first portion of the web from the assembly of the first (rigid) base and the second portion of the web, so as to separate the first and the second filters or filter units.
  • the functionality of the proposed filter stack can be evaluated by the effects of leakage over pattern borders (i.e. question whether leakage of whole blood or plasma can be observed over the THF patterned area in the filter stack), sufficient contact between filter and cartridge, cartridge filling speeds, and filtration and cartridge filling success rate.
  • a filter, a filter unit/module or a complete microfluidic diagnostic device e.g. disposable cartridge
  • a filter, a filter unit/module or a complete microfluidic diagnostic device e.g. disposable cartridge
  • the filter unit/module or the complete diagnostic microfluidic device with integrated filtration membrane have been described, wherein the filter or filter unit/module or microfluidic (diagnostic) device comprises a base portion comprising a microfluidic element to collect some filtered sample, and a web portion made of a filter material an arranged on top of the base portion, wherein the web portion comprises predetermined zones wherein the filtering function has been inhibited (e.g.
  • pores of the filter material have been collapsed in these zones or pores have been filled-up by a filling material as previously explained), which define a filtering active area covering at least a part of said microfluidic element, said collapsed pores being adapted to inhibit at least partly the filtering function of the filter material.
  • the filter function can be used in many microfluidic devices (e.g. reactors, which are not intended for diagnostic purposes).
  • a single unit or device may fulfill the functions of several items recited in the claims.
  • the mere fact that certain measures are recited in mutually different dependent claims does not indicate that a combination of these measures cannot be used to advantage.

Abstract

L'invention porte sur un filtre, sur une unité de filtre/un module ou sur un dispositif microfluidique complet (par exemple, une cartouche jetable à des fins de diagnostic) ayant une membrane de filtration intégrée pour filtrer un élément de fluide à partir d'un échantillon, et sur un procédé de fabrication de la membrane, laquelle unité de filtre/lequel module ou lequel dispositif microfluidique complet a une membrane de filtration intégrée, et lequel filtre, laquelle unité de module/lequel module ou lequel dispositif microfluidique complet comprend une partie de base comprenant un élément microfluidique pour collecter une certaine quantité d'échantillon filtré, et une partie de film (10) réalisée en un matériau de filtre disposée sur la partie de base, la partie de film (10) comprenant des zones prédéterminées (40) de pores écrasés du matériau de filtre, qui définissent une zone active de filtration recouvrant au moins une partie dudit élément microfluidique, les pores écrasés étant aptes à inhiber la fonction de filtration du matériau de filtre.
PCT/EP2014/077330 2013-12-16 2014-12-11 Réalisation de motifs sélective de membranes de filtration WO2015091189A1 (fr)

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EP13197345 2013-12-16
EP13197345.5 2013-12-16

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Cited By (1)

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CN106434302A (zh) * 2016-09-18 2017-02-22 华中科技大学 一种便携式无动力源的微流控细胞分离芯片

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