WO2015063347A1 - Appareil de commande du débit d'un dispositif microfluidique - Google Patents

Appareil de commande du débit d'un dispositif microfluidique Download PDF

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Publication number
WO2015063347A1
WO2015063347A1 PCT/ES2013/070746 ES2013070746W WO2015063347A1 WO 2015063347 A1 WO2015063347 A1 WO 2015063347A1 ES 2013070746 W ES2013070746 W ES 2013070746W WO 2015063347 A1 WO2015063347 A1 WO 2015063347A1
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WO
WIPO (PCT)
Prior art keywords
microfluidic channel
microvalve
thermosensor
portable device
section
Prior art date
Application number
PCT/ES2013/070746
Other languages
English (en)
Spanish (es)
Inventor
Aitor EZKERRA FERNÁNDEZ
Jaione ETXEBARRÍA ELEZGARAI
Jorge ELIZALDE GARCÍA
Original Assignee
Ikerlan, S. Coop.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ikerlan, S. Coop. filed Critical Ikerlan, S. Coop.
Priority to PCT/ES2013/070746 priority Critical patent/WO2015063347A1/fr
Priority to EP13803087.9A priority patent/EP3078421A1/fr
Publication of WO2015063347A1 publication Critical patent/WO2015063347A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/082—Active control of flow resistance, e.g. flow controllers

Definitions

  • the present invention is an apparatus for controlling the flow rate in a given section in a microfluidic device.
  • This apparatus is formed by a first interrelated product provided in the form of a portable device comprising microfluidic channels and a second interrelated product provided in the form of a control apparatus adapted to receive the first portable device.
  • the first portable device comprises at least one plate with at least one section of open microfluidic channel configured on the surface of the plate.
  • the same section of the microfluidic channel comprises a microvalve, either upstream of the thermosensor or downstream of the thermosensor, covered by a flexible sheet, which establishes a regulation of the flow in the stretch of microfluidic channel depending on the pressure exerted on the flexible sheet .
  • the invention allows the regulation of the flow in the section of the microfluidic channel by establishing a flow rate according to a setpoint value in the control apparatus with a closed loop regulation between the thermosensor signal and the microvalve.
  • microfluidic devices consist of a plate comprising cameras and microfluidic channels, also called microchannels, where experiments are carried out that give rise to results that would otherwise require laboratory tests. These devices are usually disposable.
  • small flow meters capable of measuring reduced flows are known.
  • they are capable of measuring very low flow rates they are not devices that can be incorporated into a microfluidic device and what is done is to arrange these devices either coupled to an input port or an output port, and always outside of the microfluidic device.
  • these micro flowmeters only measure the flow rate and do not provide a control that determines a certain flow rate value according to a setpoint value.
  • thermal sensors that measure the flow by incorporating thermal sensors. These thermal sensors are formed by a plurality of electrodes located inside the channel through which the fluid passes. Some of these electrodes are heated when they are fed by increasing the temperature of the fluid with which they are in contact. Other electrodes act as temperature sensors by measuring the temperature upstream and also downstream of the electrodes that provide heat. Depending on the flow rate, given the same heat input, the temperature increase measured in the flow will be greater or less. It is possible to establish a correlation between the temperature increase between the groups of electrodes intended for reading upstream and downstream and the flow through the electrodes determining the flow rate. However, in all cases the electrodes are located in the channel in order to be in contact with the fluid on which the flow is to be evaluated. This condition requires that known flowmeters be devices that must be incorporated in the specific place for which the microfluidic channel support has been designed together.
  • the present invention establishes a combination of sensor adapted to measure the flow rate in a section of microfluidic channel and a microvalve, placing the electrodes out of the channel.
  • This allows not only to integrate at a particular point of the microfluidic device, in particular at an intermediate point of the microfluidic path, without it having to be at an inlet or outlet port; and, in addition to establishing a control of said flow according to a preset setpoint.
  • a second technical advantage when the electrodes are placed in the control apparatus adapted to receive the microfluidic device, is the reduction of costs in the microfluidic portable device. If this portable microfluidic device is disposable, the electrodes are only necessary in the control apparatus and serve to carry out the measurements in a plurality of microfluidic devices instead of having to incorporate as many thermosensors as disposable devices.
  • the present invention is an apparatus for controlling the flow rate in a given section in a microfluidic device.
  • This apparatus is formed by a first interrelated product provided in the form of a portable device comprising microfluidic channels, typically a device called "Lab on a Chip", and a second interrelated product provided in the form of an adapted control apparatus. to receive the first portable device. It is this second control apparatus that acts on the first, the portable device, establishing a certain flow rate in a pre-established section of microfluidic channel.
  • the same control apparatus is capable of establishing a preset flow rate in different sections of the microfluidic channel of the portable device where these sections in which a certain flow rate is regulated can be intermediate sections between chambers, or between other elements and that are not necessarily in direct contact with the fluidic inlet and outlet of the microfluidic device.
  • the first interrelated product for flow control in a microfluidic device is provided in the form of a portable device and this comprises:
  • first conductive membrane thermally bonded to the support plate such that it covers the at least one section of microfluidic channel such that said section of microfluidic channel is closed by the first membrane.
  • the portable device that gives rise to the first interrelated product is mainly formed by a plate.
  • This board can contain cameras and channels inside, depending on the functions that you want the portable device.
  • it comprises an open channel section, that is, it is a channel with walls that are intended to guide the flow of a fluid sample but its section is not a closed path.
  • the open channel section is accessible from the outside before being covered by the first membrane.
  • This first membrane covers the open channel section extending along the outer surface of the plate containing the channel.
  • a particular way of applying this first membrane is by bonding it with adhesive to the plate.
  • the first membrane extends over the surface of the plate and in particular covers the open channel section resulting in a closed channel.
  • the first sheet that closes the open channel section is thermally conductive.
  • a region of the outer surface of the first sheet where the opposite side of said region is in contact with at least part of the section of the microfluidic channel is the region that will allow the reading of the flow through the microchannel section.
  • a thermosensor is arranged over this region. In an exemplary embodiment, this sensor has electrodes for generating heat. Heat is transmitted to the fluid because the first sheet is thermally conductive.
  • the thermosensor also has electrodes for reading the temperature upstream and downstream, with respect to the direction of the flow passing through the section of the microfluidic channel, of the electrodes that provide heat to the fluid.
  • the sheet is thermally conductive, it establishes a barrier to the passage of heat that can prevent the correct flow reading with the dimensions imposed on a microchannel.
  • the electrodes outside the microchannel leaving the membrane as an intermediate barrier between the electrodes and the fluid that passes through the microfluidic channel, it has been proven experimentally that the solution of incorporating the electrodes outside the sheet does not prevent the correct reading of the flow.
  • the electrodes that are placed on the surface region belong either to the portable device or to the machine in charge of carrying out the control over the portable device.
  • the electrodes can be deposited by sputtering, evaporation, screen printing, jet printing or a combination of any of them.
  • the placement of the first sheet only requires a good closing of the channels without the position requirements imposed by the electrodes being on this first sheet.
  • the second sheet does not have to cover the entire area of the first sheet so the placement of the second sheet containing the electrodes should only ensure correct positioning with respect to the region on the outer surface of the first conductive membrane intended to receive the electrodes
  • microvalve is configured according to an open cavity on the surface of the support plate
  • the open cavity has a microfluidic inlet and outlet, or the open cavity is covered by a flexible membrane such that it has a region of its outer surface adapted to receive a pressure actuator such that the pressure on said region causes deformation of the flexible membrane and the closure of the microvalve, where either the inlet or the outlet of said microvalve is in fluidic connection with the section of the microfluidic channel.
  • the flow regulation on the section of the microfluidic channel is carried out by acting on a micro valve that is in microfluidic communication with said section either upstream or downstream.
  • the microvalve is formed by an open cavity, interpreting as open a configuration equal to that of the open channel section, where the open cavity becomes closed because a flexible membrane covers it extending along the outer surface of the support plate where the cavity is located open
  • the regulation in closed loop between the flow measured in the thermosensor and the action on the microvalve establishes a flow through the section of microfluidic channel according to the set value of the closed loop.
  • the invention also has a second interrelated product provided in the form of a control apparatus.
  • This control apparatus is adapted to receive the portable device so that it is able to read the flow in the microfluidic channel section which has a region adapted to receive the thermosensor; and to act on the microvalve regulating the flow according to a preset setpoint.
  • This control apparatus comprises:
  • an actuator adapted to exert pressure on the region of the outer surface of the flexible membrane located covering the micro valve of the portable device once fixed on the fixing support and adapted to receive a pressure actuator
  • thermosensor adapted to come into contact with the region on the external surface of the first conductive membrane of the portable device once fixed on the fixing bracket; or, if the portable device already has a sensor, means for contacting said sensor when the portable device is fixed in the fixing bracket.
  • the control device receives the portable device in a fixing bracket.
  • the fixing support determines the position where either the region of the first membrane where the thermosensor is to be located or the electrodes of the thermosensor are located if the portable device has said thermosensor.
  • the thermosensor is arranged in the control apparatus in a position such that the thermosensor is in contact with the first thermally conductive membrane on the region adapted to receive the thermosensor.
  • the positioning that determines the fixing support allows the actuator intended to press on the microvalve to regulate its opening or closing is placed on the flexible membrane portion intended to admit actuator actuation by pressure.
  • thermosensor or comprising a signal input from the thermosensor where said central processing unit is adapted to determine the flow rate passing through the at least one section of microfluidic channel from the input signal, or comprising an output in connection with the actuator for the control of the microvalve,
  • central processing unit is configured in closed loop to regulate the flow through the micro valve to reach the setpoint value.
  • the central processing unit coordinates at least the flow reading by means of the thermosensor and the action on the actuator that establishes the degree of opening or closing of the microvalve according to a closed loop scheme.
  • This same central processing unit can manage a plurality of sensors and valves so that closed loop regulation can be carried out on a path containing a thermosensor, a valve, closing the rest of valves in such a way that the aforementioned path is established.
  • FIG 1 This figure shows a plan and elevation view of a microfluidic device according to a first embodiment comprising a channel on which a certain flow is to be imposed, a section of channel with a thermosensor for reading the flow rate , a section in bypass to increase the flow that is capable of regulating the microfluidic device; and, a microvalve.
  • the elevation view is shown immediately below the plan view with the membrane located slightly apart from the plate to distinguish both elements.
  • Figure 2a Figure 2a shows a first embodiment of a micro valve with a mechanical actuator.
  • Figure 2b shows a second embodiment of a micro valve with a pneumatic actuator.
  • Figure 3 shows a perspective of an embodiment of an open channel section, closed by a thermally conductive sheet on which the part of the electrodes that is active in the flow reading is shown.
  • Figure 4 is a diagram showing an exemplary embodiment of a control apparatus adapted to receive a portable device so that, once introduced into the control apparatus, it is possible to establish a certain flow rate in a stretch of microfluidic channel in said portable device.
  • Figure 5 shows a diagram of an exemplary embodiment of a portable device comprising a drive pump, either arranged integrated in the portable device, or external to said portable device, whose output is in fluidic communication with three sections, each of them comprising a sensor and a valve.
  • a drive pump either arranged integrated in the portable device, or external to said portable device, whose output is in fluidic communication with three sections, each of them comprising a sensor and a valve.
  • a flow regulation in any of the three branches of the portable device.
  • Figure 6 shows a diagram of an exemplary embodiment of a portable device comprising two possible inputs and a single output.
  • the fluid inlet is selected and, on this inlet, the flow regulation is carried out.
  • Figure 7 shows a diagram of an exemplary embodiment of a portable device comprising a single fluid inlet and two branches leading to each outlet.
  • the fluid outlet is selected and, on the branch that flows into said outlet, the flow regulation is carried out.
  • Figure 8 Figure 8 shows a graph in which three curves overlap.
  • the continuous line curve is a stepped function with the setpoint flow value imposed on a control device acting on a portable device.
  • the dashed curve is the flow response obtained before the imposition of the setpoint value in an embodiment of the invention, obtaining the reading by means of a thermosensor according to the invention arranged on the thermally conductive membrane.
  • the curve drawn in points is the response to the measurement flow using a commercial micro flow meter.
  • the present invention is an apparatus consisting of a first interrelated product and a second interrelated product.
  • the first interrelated product is the portable device (1) and the second interrelated product is the control device (2) that receives the portable device (1) to act on it (1) achieving that at least in a section of microfluidic channel ( 1.4) pass a preset flow as the setpoint value.
  • the plate (1.1) in which microfluidic channels and other cavities such as those giving rise to a microvalve (1.6) are distinguished; and, distanced from the plate (1.1), a membrane (1.2).
  • the membrane (1.2) is a thermally conductive sheet and is also flexible.
  • the same membrane (1.2) allows to establish the closure of the section of the microfluidic channel (1.4) on which the thermosensor (1.7.1) is arranged and also the closure of the microvalve cavity (1.6) with flexibility which allows the regulation of the degree of opening of said microvalve (1.6) by the deformation it suffers according to the pressure exerted on it.
  • the membrane (1.2) extends over one side of the plate (1.1) and is attached to it by means of an adhesive.
  • the flow rate reading in the narrow channel section (1.4) determines the flow rate in the channel section (1.5) bypass as the ratio of sections is known.
  • thermosensor (1.7.1) is formed by three electrode sections arranged on the membrane (1.2) as shown in detail in the embodiment shown in Figure 3.
  • a central electrode produces a pre-established amount of heat when It makes current flow through you. The heat it produces is transferred to the flow that passes through the channel through the membrane (1.2) since it is conductive to the passage of heat.
  • the electrodes arranged on the sides of this electrode intended to generate heat allow the temperature to be read before and after the heat is supplied. The temperature difference will be smaller the greater the flow that passes through the channel. The correlation between this temperature difference and the flow allows to measure the flow that passes through the microfluidic channel located under the thermosensor (1.7.1).
  • Figure 1 shows conductive tracks (1.7) located on the membrane (1.2) that establish the electrical communication between the supply and reading contacts, and the three electrodes acting as thermosensor (1.7.1) that are located on the microfluidic channel .
  • the conductive tracks (1.7) and in particular the electrodes acting as a thermosensor (1.7.1) are arranged on an adhesive sheet other than the membrane (1.2) and adheres on it in such a way that the electrodes acting as a thermosensor (1.7.1) they are properly positioned on the microfluidic channel in which the flow measurement is carried out.
  • the conductive tracks (1.7) and in particular the electrodes acting as thermosensor (1.7.1) are deposited by means of "sputtering", evaporation, screen printing, jet printing or a combination of any of them.
  • microvalve (1.6) is configured by means of a cavity (1.6.3) to which the inlet (1.6.1) arrives.
  • the outlet (1.6.2) is arranged at the bottom of the cavity (1.6.3) where in this embodiment the bottom shows a concavity.
  • the membrane (1.2) is pressed on its outer face by an actuator (3, 4) that exerts pressure.
  • the actuator is a bar that ends on a blunt surface and adapts to the concavity of the bottom of the cavity (1.6.3).
  • the actuator is formed by a chamber (4) that has a support (4.1) to achieve the seal when the membrane (1.2) is pressed externally; and, which has a conduit (4.2) for the injection of a gas, for example pressurized air.
  • the gas pressure is what causes the membrane to descend (1.2), deforming and causing the major or minor closure of the microvalve (1.6).
  • the regulation of the degree of opening of the microvalve (1.6) in this exemplary embodiment is carried out by managing the pressure introduced into the chamber (4) of the actuator.
  • FIG 4 schematically shows a portable device (1) that is inserted into the control apparatus (2). Once inserted and placed in the control device (2), the portable device (1) is positioned on the fixing support of the control device (2) where it (2) has at least one sensor module (S) that carries out the flow rate reading through the thermosensor (1.7.1).
  • the sensor module (S) has means of reading the value provided by the thermosensor (1.7.1) or, if there are several, of each of them.
  • It also has at least one actuator module (A), which acts on at least one microvalve (1.6) of those arranged in a section of microfluidic channel (1.3) to regulate the pre-established flow rate by entering a value in the control device (2) of setpoint. Likewise, if there are several microvalves (1.6), the actuator module (A) is capable of acting differently in each of them.
  • a central processing unit (CPU) receives the signal that comes from the at least one sensor module (S) and acts on the at least one actuator module (A) according to a closed loop regulation. That is, before a setpoint value, this setpoint value is compared with the value of the flow read by the sensor module (S). If the value of the flow rate read is greater than the setpoint value then the degree of actuator actuation (3, 4) that closes the microvalve (1.6) is increased. If, on the contrary, the value of the read flow rate is lower than the setpoint value then the degree of actuator actuation (3, 4) that closes the microvalve (1.6) is reduced to allow a greater flow rate.
  • Figure 5 shows a scheme of channels and microfluidic components according to an exemplary embodiment.
  • the portable device is powered by drive means (B).
  • the drive means are either integrated in the microfluidic device or are external to the portable device.
  • the output of the drive means (B) is in fluidic communication with three microfluidic channels in which, each of them, comprises a microvalve (1.6) and a thermosensor (1.7.1).
  • the control apparatus (2) for each of the microfluidic channels, performs a closed loop control by reading the flow rate on the microfluidic channel and acting on the micro valve (1.6) located in the same channel.
  • control unit processes in parallel a regulation on each of the channels so that it is possible to preset a different flow rate for each channel.
  • control unit processes a single closed loop control over one of the channels and, by means of the actuator module (A), keeps the other microvalves (1.6) closed.
  • the drive means can be a pump or a source of constant pressure flow.
  • Figure 6 shows a scheme of channels and microfluidic components according to another embodiment.
  • the portable device is powered by two different fluidic inlets.
  • Each of the fluidic inlets has a micro valve (1.6).
  • the output of each of the microvalves (1.6) is in communication with a single channel that has a sensor (1.7.1). This channel is the output.
  • This scheme can be generalized with a plurality of inputs each with a micro valve (1.6) that communicates with the sensor (1.7.1).
  • control apparatus (2) comprises a central processing unit (CPU) that is adapted to establish the closure of all microvalves (1.6) except one of them leaving a single possible path and therefore a single path. fluid inlet
  • CPU central processing unit
  • the same central processing unit (CPU) establishes the regulation of the channel that follows the only possible path by reading the flow in the sensor (1.7.1) and the action on the microvalve (1.6) that is not necessarily closed.
  • Figure 7 shows another embodiment in which there is a common fluid inlet to two channels.
  • the channel section corresponding to the common inlet has a main microvalve (1.6) and each channel that starts from this common inlet comprises a thermosensor (1.7.1) and a microvalve (1.6).
  • This scheme is generalizable by extending the number of two channels to a plurality of channels fed by the same input.
  • the control apparatus adapted to control the portable device according to this microfluidic scheme carries out a control that closes all the microvalves located in the individual microfluidic channels fed by the common input, except for a preset.
  • the open valve defines a single microfluidic path whose flow is determined with a closed loop control using the main microvalve (1.6) and the sensor (1.7.1) arranged in the channel that has its microvalve (1.6) open.
  • control section of the examples shown in figures 5, 6 and 7 may choose to change the valve configuration by defining another alternative path and therefore the combination of sensor (1.7.1) and microvalve (1.6) with the that carry out the control in closed loop.
  • the manufacture of electrodes that are deposited on the outer face of the membrane (1.2) allows sensing and flow control to be placed in any part of the microfluidic device or even in a plurality of places avoiding the design of input and output ports for coupling dedicated devices for the measurement or flow control.
  • the combination of a portable device and the device adapted to operate on said portable device is also object of this invention when the portable device is compatible in its configuration with the control device.
  • At least one experiment has been carried out where the capacity of response and flow regulation in a microchannel according to the invention is tested in the laboratory.
  • the experiment consists in establishing on the microchannel a flow determined by a setpoint value that follows an increasing step function.
  • Figure 8 shows in continuous line the increasing function according to staggered sections.
  • the response to the flow has been measured experimentally by two methods, a first method that makes use of the signal obtained in the thermosensor of the thermosensor of the portable device itself and a second method that makes use of a commercial flow meter arranged at the output of the built-in portable device how a device in series with the output of the microchannel.
  • Figure 8 shows in broken lines the response obtained according to the first method and in dotted lines the response measured by the second method.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micromachines (AREA)

Abstract

La présente invention concerne un appareil de commande du débit dans une trame déterminée d'un dispositif microfluidique. Cet appareil comprend un premier produit apparenté ayant la forme d'un appareil de commande conçu pour recevoir le premier dispositif portatif. Le premier dispositif portatif comprend au moins une plaque avec au moins une trame de canal microfluidique ouvert formée sur la surface de la plaque. Sur cette trame de canal microfluidique se trouve une couche thermiquement conductrice qui ferme le canal microfluidique ouvert qui comprend une zone sur la surface externe qui reçoit un thermocapteur. Cette même trame de canal microfluidique comprend une microvanne, ou des eaux sur le thermocapteur ou des eaux sous le thermocapteur, recouverte d'une couche souple, qui établit une régulation du flux dans la trame du canal microfluidique en fonction de la pression exercée sur la couche souple. L'invention permet de réguler le flux dans la trame du canal microfluidique en établissant un débit selon une valeur de consigne dans l'appareil de commande avec une régulation en boucle fermée entre le signal du thermocapteur et la microvanne.
PCT/ES2013/070746 2013-10-29 2013-10-29 Appareil de commande du débit d'un dispositif microfluidique WO2015063347A1 (fr)

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PCT/ES2013/070746 WO2015063347A1 (fr) 2013-10-29 2013-10-29 Appareil de commande du débit d'un dispositif microfluidique
EP13803087.9A EP3078421A1 (fr) 2013-10-29 2013-10-29 Appareil de commande du débit d'un dispositif microfluidique

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PCT/ES2013/070746 WO2015063347A1 (fr) 2013-10-29 2013-10-29 Appareil de commande du débit d'un dispositif microfluidique

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CN112673090A (zh) * 2018-10-29 2021-04-16 Nok株式会社 微流控芯片及微流控器件

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WO2008079900A1 (fr) * 2006-12-20 2008-07-03 Applied Biosystems, Llc Dispositifs et procédés de gestion de l'écoulement dans des structures microfluidiques
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