WO2015037328A1 - Support d'immobilisation microbienne - Google Patents

Support d'immobilisation microbienne Download PDF

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Publication number
WO2015037328A1
WO2015037328A1 PCT/JP2014/069014 JP2014069014W WO2015037328A1 WO 2015037328 A1 WO2015037328 A1 WO 2015037328A1 JP 2014069014 W JP2014069014 W JP 2014069014W WO 2015037328 A1 WO2015037328 A1 WO 2015037328A1
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WO
WIPO (PCT)
Prior art keywords
microorganism
sludge
immobilized carrier
foam
carrier
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PCT/JP2014/069014
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English (en)
Japanese (ja)
Inventor
小林光廣
武部尚市
鈴木真隆
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新日本フエザーコア株式会社
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Publication of WO2015037328A1 publication Critical patent/WO2015037328A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C02F3/10—Packings; Fillings; Grids
    • C02F3/105—Characterized by the chemical composition
    • C02F3/108—Immobilising gels, polymers or the like
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/093—Polyurethanes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/10—Biological treatment of water, waste water, or sewage

Definitions

  • the present invention relates to a microorganism-immobilized carrier for supporting microorganisms in a method of treating sludge using microorganisms.
  • sludge and activated sludge are mixed in an aeration tank, aerated and aerobic microorganisms in the activated sludge decompose the pollutants in the sludge, and the activated sludge proliferated and purified treated water and Is a method of separating.
  • Patent Document 1 proposes an invention in which, in the activated sludge method as described above, a carrier that holds aerobic microorganisms is introduced, and the carrier to which the microorganisms adhere is caused to flow in an aeration tank to treat the sludge.
  • Patent Document 2 proposes an invention relating to a microorganism fixing carrier that has an appropriate buoyancy in water such as drainage and has both excellent microbial retention and durability.
  • FIG. 7 is a diagram showing an example of a conventional microorganism-immobilized carrier used in the activated sludge method.
  • the aerobic microorganisms in the activated sludge can be obtained by introducing a cubic microorganism-immobilized support 15 made of a porous foam (for example, a resin sponge) illustrated in FIG. 7 into the sludge in the aeration tank. It is carried on the microorganism immobilization carrier 15.
  • microorganism-immobilized carrier various performances are required for the microorganism-immobilized carrier.
  • One of them is sludge retention.
  • a resin sponge material, a soft material such as a gel, or a hard material such as a straw is used to increase the amount of sludge retained.
  • microorganism-immobilized carrier made of such a material has an advantage that a large amount of sludge is retained, there is a problem that sludge accumulated on the carrier is difficult to be discharged.
  • the microbial immobilization carrier 15 collides with the wall surface in the aeration tank and other microbial immobilization carriers due to the flow of sludge, and for example, external forces 16a, 16b, 16c, 16d, 16e, and 16f are generated.
  • sludge in the vicinity of each outer peripheral surface (upper surface, bottom surface, side peripheral surface) of the microorganism-immobilized carrier 15 is, for example, arrows 17a, 17b, 17c, 17d, 17e, 17f, 17k, 17l. , 17m, 17n (FIGS. 7A and 7B).
  • the microorganism-immobilized carrier 15 is used for a long time with sludge remaining in the central portion 15a, the inside of the microorganism-immobilized carrier 15 is changed from an aerobic state to an anaerobic state, and the aerobic microorganisms carried are reduced. In addition, the sludge treatment performance may be reduced.
  • microorganism-immobilized carrier 15 is likely to wear because it flows in the aeration tank and collides with the wall surface in the aeration tank and other microorganism-immobilized carriers.
  • the replacement frequency of the microorganism-immobilized carrier increases, which may hinder stable sludge purification treatment.
  • an object of the present invention is to maintain the aerobic state of the central part of the microorganism-immobilized carrier, to improve the wear resistance, and to provide a microorganism-immobilized carrier having such characteristics.
  • the invention of claim 1 Among the plurality of openings formed on the surface of the three-dimensional structure made of a porous foam, at least two of the openings are provided, and a hollow portion passing through the inside of the three-dimensional structure is provided. It is a microorganism immobilization carrier.
  • the invention of claim 2 The microorganism-immobilized carrier according to claim 1, wherein the hollow portion passes through the center of gravity of the three-dimensional structure.
  • the invention of claim 3 2.
  • the specific surface area is increased by 10% to 20% compared to the three-dimensional structure in which the cavity is not formed because the cavity is formed.
  • the invention of claim 4 The microorganism-immobilized carrier according to any one of claims 1 to 3, wherein an annular film is formed on an inner peripheral wall of a cell forming the porous structure of the foam.
  • the present invention it is possible to maintain the aerobic state of the central part of the microorganism-immobilized carrier, improve the wear resistance, and provide a microorganism-immobilized carrier having such characteristics.
  • carrier of this invention is illustrated, Comprising: (a) The perspective view showing a 1st example, (b) The perspective view showing a 2nd example, (c) The perspective view showing a 3rd example (D) It is a perspective view showing the 4th example, (e) It is a perspective view showing the 5th example. It is a figure showing the state which external force worked to the microorganisms immobilization support shown in Drawing 1 (a), and the state where sludge is discharged, (a) a perspective view and (b) a front view. It is a conceptual diagram showing an example in which sludge treatment is performed using the microorganisms immobilization carrier of the present invention.
  • FIG. 1 illustrates a microorganism-immobilized carrier of the present invention.
  • the microorganism-immobilized carrier comprising the porous foam of the present invention shown in FIG. 1 carries aerobic microorganisms in activated sludge in an aeration tank, and is a water-swellable polyurethane known in this technical field. It can be manufactured by processing synthetic resin such as foam, ceramics, inorganic substances such as silica gel, sponge and the like.
  • the foam 2 made of a synthetic sponge material such as urethane sponge is processed into the microorganism-immobilized carriers 1a and 1b made of a cubic three-dimensional structure of 10 mm ⁇ 10 mm ⁇ 10 mm (FIG. 1A).
  • the microorganism-immobilized carriers 1a and 1b made of a cubic three-dimensional structure of 10 mm ⁇ 10 mm ⁇ 10 mm (FIG. 1A).
  • microbial immobilization carriers 1c, 1d, and 1e made of a rectangular solid-shaped three-dimensional structure are illustrated (FIGS. 1C, 1D, and 1E).
  • the shape of the microorganism-immobilized carrier 1a, 1b, 1c, 1d, and 1e made of the synthetic sponge material is not limited to a cube or a rectangular parallelepiped as long as a cavity to be described later can be formed therein.
  • Various three-dimensional structures such as a body, a cylinder, and a sphere can be used.
  • a resin sponge that becomes a gel during swelling can also be employed.
  • the number of cells forming the porous structure of the microorganism-immobilized carriers 1a, 1b, 1c, 1d, and 1e is preferably 10 to 50/25 mm. If the number of cells exceeds 50, it becomes a fine carrier and tends to carry aerobic microorganisms, but it tends to wear. On the other hand, when the number of cells is less than 10, it becomes a coarsely foamed carrier and it is difficult to carry aerobic microorganisms. Therefore, the number of cells is preferably 10 to 50/25 mm.
  • the microorganism-immobilized carrier of the present invention comprises the above-described three-dimensional structure, and a plurality of openings are formed on the surface of the three-dimensional structure. And the hollow part which connects at least 2 of these opening parts and passes through the inside of the said three-dimensional structure is provided.
  • an opening 4, 4 having a diameter of 4 mm is provided at the center of the front surface 2 a and the back surface 2 b of the cubic foam 2, passing through the center of gravity G of the foam 2, and the opening 4, 4.
  • a cylindrical hollow portion 3 a having a length of 10 mm is formed in the foam 2.
  • an opening 4, 4 having a diameter of 4 mm is provided at the center of the front surface 2 a and the back surface 2 b of the cubic foam 2, passing through the center of gravity G of the foam 2, and opening 4, 4
  • a cylindrical hollow portion 3 a having a length of 10 mm is formed in the foam 2.
  • an opening 4 or 4 having a diameter of 4 mm is provided at the center of the upper surface 2c and the lower surface 2d of the foam 2, and a cylindrical hollow portion having a length of 10 mm that passes through the center of gravity G of the foam 2 and connects the openings 4 and 4 is provided.
  • 3 b is formed inside the foam 2.
  • an opening 4, 4 is provided at the center of the front surface 2 a and the back surface 2 b of the rectangular parallelepiped foam 2, passes through the center of gravity G of the foam 2, and connects the openings 4, 4.
  • a columnar cavity 3 c is formed inside the foam 2.
  • the openings 4, 4 are provided at the centers of the front surface 2 a and the back surface 2 b of the rectangular parallelepiped foam 2, and the circles that pass through the center of gravity G of the foam 2 and connect the openings 4, 4
  • a columnar cavity 3 c is formed inside the foam 2.
  • an opening 4, 4 is provided in the center of the left side 2 e and the right side 2 f of the foam 2, and a cylindrical hollow part 3 d that passes through the center of gravity G of the foam 2 and connects the openings 4, 4 is the foam 2. Is formed inside.
  • openings 4, 4 are provided at the center of the front surface 2 a and the left side surface 2 e of the rectangular parallelepiped foam 2, and the openings 4, 4 are connected through the center of gravity G of the foam 2.
  • An L-shaped cavity 3 e is formed inside the foam 2.
  • a cavity connecting openings formed on different surfaces of the three-dimensional structure passes through the inside of the three-dimensional structure.
  • a cavity connecting openings formed on different surfaces of the three-dimensional structure passes through the inside of the three-dimensional structure.
  • two openings are formed on one surface of a three-dimensional structure, the two openings are connected, and a cavity passing through the center of gravity of the three-dimensional structure is a three-dimensional structure. It is also possible to make a structure that passes through the inside of the.
  • the hollow portions 3a, 3b, 3c, 3d, and 3e that connect at least two of the openings formed on the surface of the three-dimensional structure and pass through the inside of the three-dimensional structure are described below. , It plays a role of discharging sludge remaining inside the microorganism-immobilized carriers 1a, 1b, 1c, 1d, 1d, and 1e.
  • the microorganism-immobilized carrier 1a collides with the wall surface in the aeration tank or other microorganism-immobilized carrier due to the flow of sludge containing sewage or the like to be treated in the aeration tank.
  • external forces 5a, 5b, 5c, 5d When 5e and 5f are added in the directions of the respective arrows, sludge in the vicinity of each outer peripheral surface (upper surface, bottom surface, side peripheral surface) of the microorganism-immobilized support 1a is, for example, arrows 6a, 6b, 6c, 6d, 6e, and 6f. , 6g, 6h, 6i, 6j.
  • a part of the sludge flows into the inside of the microorganism immobilization carrier 1a in the directions of arrows 6k, 6l, 6m, and 6n, for example, but the sludge that has flowed into the interior is formed by the cavity 3a. It is also discharged in the directions of arrows 6o and 6p through 3a and openings 4 and 4.
  • the inside of the microorganism immobilization carrier 1a is maintained in an aerobic state. If the aerobic state is maintained, the supported aerobic microorganisms can be retained in the microorganism-immobilized carrier 1a for a long period of time.
  • the aerobic microorganisms are immobilized through the openings 4, 4 and the cavity 3a by the amount added. It becomes easy to carry
  • Table 1 shows the comparison.
  • the increased specific surface area makes it easier to support aerobic microorganisms inside the microorganism-immobilized carrier through the openings and cavities, and sludge and aerated air can also be contained in the microorganism-immobilized carrier through the openings and cavities. Therefore, the growth of the supported aerobic microorganisms can be promoted.
  • the microorganism immobilization carrier 1a collides with the wall surface in the aeration tank and other microorganism immobilization carriers due to the flow of sludge including sewage to be treated in the aeration tank, for example, external force 5a, When 5b, 5c, 5d, 5e, and 5f are added in the directions of the respective arrows, these external forces can be relaxed by the cavity 3a.
  • the microorganism-immobilized carrier of the present embodiment is provided with a hollow portion that connects at least two of the openings formed on the surface of the three-dimensional structure and passes through the inside of the three-dimensional structure. It is preferable that the size of the hollow portion is such that the specific surface area is increased by 10% to 20% as compared with the three-dimensional structure in which the hollow portion is not formed.
  • the opening diameter of the opening 4 is 4 mm, but the above-described three-dimensional structure in which the cavity is not formed due to the formation of the cavity, As long as the specific surface area is increased by 10% to 20%, the opening diameter of the opening 4 may be less than 4 mm or 4 mm or more. Considering the shape and dimensions of the microorganism-immobilized carrier, the specific surface area is 10% to 20% compared to the three-dimensional structure in which the hollow portion is not formed because the hollow portion is formed.
  • the opening diameter of the opening 4 can be changed in various ways within a range that increases by%.
  • the hollow portions 3a to 3e all pass through the center of gravity G of the three-dimensional structure.
  • the present invention is not limited to this, and the hollow portion connecting at least two of the openings formed on the surface of the three-dimensional structure and passing through the inside of the three-dimensional structure is the center of gravity of the three-dimensional structure.
  • a structure that does not pass through G may be used.
  • the cavities 3a to 3e have a structure / form that passes through the center of gravity G of the three-dimensional structure.
  • the microorganism-immobilized carrier of this embodiment consisting of a synthetic sponge material such as urethane sponge, a synthetic resin such as water-swellable polyurethane foam, ceramics, inorganic substances such as silica gel, and a three-dimensional structure manufactured by processing sponge
  • the center of gravity G and its center often coincide. Therefore, a hollow portion connecting at least two of the openings formed on the surface of the three-dimensional structure and passing through the inside of the three-dimensional structure should pass through the center of gravity G of the three-dimensional structure.
  • the sludge is efficiently discharged from the inside of the microorganism-immobilized carrier through the cavity and the opening, and the sludge remaining inside the microorganism-immobilized carrier is further removed. Can be reduced.
  • FIG. 3 is a conceptual diagram showing an example in which sludge treatment is performed using the microorganism-immobilized carrier 1a.
  • the sludge containing the sewage etc. which are the process target in the aeration tank 7 in which the diffuser 8 was provided in the bottom part circulates and flows along the direction of arrow 10a, 10b, 10c, 10d.
  • a large number of bubbles 9 are supplied from the air diffuser 8 into the aeration tank 7.
  • the microorganism-immobilized carrier 1a which absorbs sludge and dissolved air and has a biofilm room inside, descends in the aeration tank 7 along the arrow 10a from the state in which it is put in the aeration tank 7. As a result, the pressure of water pressurized in each direction of the arrow 11a increases, and a pressure state in which bubbles 9a are generated from the surface of the microorganism-immobilized carrier 1a is reached (pressure state B).
  • the sludge absorbed inside is easily discharged through the hollow portion 3a and the openings 4 and 4 in each direction of the arrow 12a including the arrows 6o and 6p illustrated in FIG.
  • the sludge remaining in the microorganism-immobilized carrier 1a is reduced compared to the microorganism-immobilized carrier 15 in which the cavity shown in FIG. 7 is not formed, and the inside of the microorganism-immobilized carrier 1a is maintained in an aerobic state. Is done. If the aerobic state is maintained, the supported aerobic microorganisms can be retained in the microorganism-immobilized carrier 1a for a long period of time.
  • the arrow 5e shown in FIG. Sludge and dissolved air are easily absorbed in each direction of the arrow 12b including 5f.
  • the specific surface area of the microorganism-immobilized support 1a is increased by forming the cavity 3a, more aerobic microorganisms are immobilized by the increased specific surface area through the openings 4, 4 and the cavity 3a. It can be carried inside the chemical carrier 1a.
  • the supported aerobic microorganisms are in contact with the absorbed sludge and dissolved air in an aerobic state inside the microorganism-immobilized carrier 1a, the growth of the aerobic microorganisms can be promoted. .
  • FIG. 4 (a) shows one form of the wall structure of the cell forming the porous structure of the microorganism-immobilized support 1a in this embodiment.
  • each cell 13 has a hexagonal shape.
  • a sludge-treated biofilm is formed on the inner peripheral wall surface of each cell 13 of this hexagonal wall structure, and aerobic microorganisms are carried thereon.
  • each cell 13 of the wall structure is not limited to the hexagonal shape shown in FIG. 4A, but various shapes such as a polygonal shape such as a triangle and a quadrangle (square, rectangle, rhombus), a circular shape, and an elliptical shape.
  • a polygonal shape such as a triangle and a quadrangle (square, rectangle, rhombus), a circular shape, and an elliptical shape.
  • a wall structure having a shape can be obtained.
  • FIG. 4 (b) is a diagram showing a structure in which an annular membrane 14 is formed on the inner peripheral wall 13a of each cell forming the porous structure of the microorganism-immobilized carrier of the present invention.
  • an annular film 14 is formed on the inner peripheral wall surface 13a of each cell 13 of the hexagonal wall structure described in FIG. 4A.
  • the annular film 14 can be formed on the inner peripheral wall 13a using burrs generated during the manufacturing process of the foam. The reason why the annular membrane 14 is formed is to increase the number of aerobic microorganisms to be carried.
  • FIG. 5 shows a hollow portion (diameter 4 mm, long, connecting two openings formed on the surface of a three-dimensional structure (12 mm ⁇ 12 mm ⁇ 12 mm) made of a porous foam and passing through the inside of the three-dimensional structure.
  • FIG. 5 (a) shows the state of the cell before the sludge treatment biofilm adheres, and a thin annular membrane such as water scraping is formed on the inner peripheral wall of each cell forming the wall structure.
  • FIG. 5 (b) is a photograph showing the state after the sludge treatment biofilm adheres to a thin annular film such as water scrap formed on the inner peripheral wall of each cell shown in FIG. 5 (a). The state in which the sludge treatment biofilm is formed on the entire inner peripheral wall (thin annular membrane) of each cell while maintaining the vent holes and water holes is shown.
  • FIG. 6 shows two hollow portions formed on the surface of a three-dimensional structure (10 mm ⁇ 10 mm ⁇ 10 mm) made of a porous foam, and a cavity (diameter 4 mm, passing through the inside of the three-dimensional structure).
  • carrier of this invention of the form illustrated in FIG. is there.
  • the number of cells is 18/25 mm.
  • FIG. 6 (b) is a photograph showing the state after the sludge treatment biofilm adheres to the inner peripheral wall of each cell of the embodiment shown in FIG. 6 (a). Compared with the embodiment shown in FIG. 5B, a state in which a sludge treatment biofilm is formed on a part of the inner peripheral wall of the cell is shown.
  • the treatment was performed using the microorganism-immobilized carrier of the present invention having the structure shown in FIGS.
  • Both the microorganism-immobilized carrier of the present invention having the structure shown in FIG. 5 and the microorganism-immobilized carrier of the present invention having the structure shown in FIG. 6 were charged into the aeration tank in a quantity that accounts for 30% of the volume of the aeration tank. .
  • the MLSS concentration was measured and found to be about 4,900 mg / L in the form shown in FIG. 5 and about 3,000 mg / L in the form shown in FIG.

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Abstract

　Le but de la présente invention est de maintenir un état aérobie au niveau de la partie centrale d'un support d'immobilisation microbienne, pour améliorer la résistance à l'usure, et pour fournir un support d'immobilisation microbienne ayant ces caractéristiques. Le support d'immobilisation microbienne est caractérisé en ce qu'il est pourvu d'une portion de cavité passant à travers l'intérieur d'une structure tridimensionnelle, et connectant au moins deux d'une pluralité d'ouvertures formées sur la surface de la structure tridimensionnelle, qui comprend une mousse poreuse.　
PCT/JP2014/069014 2013-09-12 2014-07-17 Support d'immobilisation microbienne WO2015037328A1 (fr)

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JP2006175311A (ja) * 2004-12-21 2006-07-06 Kansai Paint Co Ltd 微生物保持用担体
JP2009220079A (ja) * 2008-03-18 2009-10-01 Inoac Corp 水処理担体

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