WO2015035949A1 - Tube microcentrifuge - Google Patents

Tube microcentrifuge Download PDF

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Publication number
WO2015035949A1
WO2015035949A1 PCT/CN2014/086489 CN2014086489W WO2015035949A1 WO 2015035949 A1 WO2015035949 A1 WO 2015035949A1 CN 2014086489 W CN2014086489 W CN 2014086489W WO 2015035949 A1 WO2015035949 A1 WO 2015035949A1
Authority
WO
WIPO (PCT)
Prior art keywords
opening
tubular body
lid
microcentrifugal tube
hinge
Prior art date
Application number
PCT/CN2014/086489
Other languages
English (en)
Inventor
Weiming Yu
Original Assignee
Gene Era Biotech Co. Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gene Era Biotech Co. Ltd. filed Critical Gene Era Biotech Co. Ltd.
Priority to CN201480062387.1A priority Critical patent/CN106061612B/zh
Publication of WO2015035949A1 publication Critical patent/WO2015035949A1/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures

Definitions

  • the present invention generally relates to laboratory sample containers, and more particularly to microcentrifugal tubes.
  • Microcentrifugal tubes are small plastic tubes which are typically tapered, conical or rounded, and closed at one end.
  • the tubes are capable of holding a small amount of liquid, for example, 0.1-2.0 ml liquid, and are constructed to withstand forces typically over 10, 000 times their own weight during centrifugation. These tubes are widely used in biotechnology laboratories as vessels for storing biochemicals, performing biochemical reactions, or handling sterile contaminant-free samples.
  • a tight fitting lid sized and shaped to cover and seal the tube opening is provided with the microcentrifugal tube to maintain the inside of the microcentrifugal tube in an aseptic condition.
  • the lid is generally attached to the tube by a flexible hinge.
  • the lid may be released from the tube opening, causing liquid leakage from the microcentrifugal tube.
  • a microcentrifugal tube with improved seal has a tubular body defining a receiving cavity and a lid.
  • the tubular body is tapered to a close at a distal end and has an opening at a proximal end, wherein the tubular body has a flange extending from a first edge portion of the opening.
  • the lid is configured to seal the opening.
  • the lid has a first hooking member and a second hooking member extending from the lid.
  • the microcentrifugal tube further has a hinge. The hinge extends at a first end from a second edge portion of the opening and is attached at a second end to the lid.
  • the hinge has a through hole disposed between the first and second end of the hinge, whereby when the lid seals the opening of the tubular body, the first hooking member engages the flange of the tubular body and the second hooking member engages the hinge at the through hole, and preferably at least partially passes through the through hole.
  • the lid With the two hooking members disposed on the lid, the lid can be securely locked with the tubular body during centrifugation as the engagement of the first hinge member with the flange balances with the engagement of the second hinge member. This significantly improves the seal of the microcentrifugal tube and prevents unwanted liquid leakage from the microcentrifugal tube.
  • the first hooking member and the second hooking member protrude from a lower surface of the lid and bend towards a same direction.
  • the hooking members bending towards the same direction make it easy to release the lid from the tubular body.
  • the hinge is formed by a flexible material.
  • the lid has a sealing member protruding from its lower surface and being inserted into the tubular body through the opening when the lid seals the opening.
  • the sealing member is sized to form interference fit with the opening of the tubular body when the lid seals the opening.
  • the opening of the tubular body comprises a lead-in surface. This helps the insertion of the sealing member into the inside of the tubular body.
  • the flange comprises one or more slots at its lower surface.
  • the slots of the flange can mate with a supporting centrifuge mechanism during the centrifugation, thereby avoiding the rotation of the microcentrifugal tube relative to the supporting centrifugal mechanism.
  • the one or more slots are uniformly circumferentially spaced around the opening. In certain embodiments, the flange is disposed around an entire circumference of the opening.
  • the first edge portion is opposite to the second edge portion.
  • Figure 1 is a perspective view of a microcentrifugal tube according to an embodiment of the invention.
  • Figures 2-5 illustrates horizontal cross sectional views of the microcentrifugal tube in Figure 1 at different states from when the lid does not seal the tubular body to when the lid seals the tubular body.
  • the microcentrifugal tube 100 includes a tubular body 102 defining a receiving cavity 104.
  • the receiving cavity 104 is used to contain liquids such as water, solutions, aerosols, suspensions or the like. Biochemical, chemical or other materials may be dispersed in the liquid contained within the receiving cavity 104.
  • the tubular body 102 is generally formed by transparent or semi-transparent materials such as polymer materials, thereby a user may determine the volume of the liquid by visual inspection.
  • tubular body 102 examples include, but are not limited to, polypropylene (PP) , polycarbonate (PC) , polyvinyl chloride (PVC) , acrylonitrile butadiene styrene (ABS) , and polystyrene (PS) .
  • Scales may be provided at an outer wall 106 of the tubular body 102 to indicate the volume of the liquid.
  • the tubular body 102 is tapered to a close at a distal end 108, and has an opening 110 at a proximal end 112 opposite to the distal end 108.
  • Tubular body 102 has a flange 114 extending from a first edge portion 116 of the opening 110.
  • the microcentrifugal tube 100 further includes a lid 118 for sealing the opening 110, and a hinge 120 for connecting the lid 118 with the tubular body 102.
  • the lid 118 can cover the opening 110 to hermetically seal liquid within the receiving cavity 104 during centrifugation, and be released from the opening 110 to enable removal or addition of liquid before or after the centrifugation.
  • the hinge 120 extends at a first end 122 from a second edge portion 124 of the opening 110, and is attached at a second end 126 to the lid 118.
  • the first edge portion 116 may be opposite to the second edge portion 124.
  • the hinge 110 may be formed by a flexible material such as elastic polymer materials.
  • the hinge 120 may be a thin, rectangular piece of elastic material integrally formed with the tubular body 102 and/or the hinge 120. Alternatively, the hinge 120 may be separately formed and attached to the lid 118 and the tubular 102 by adhesives.
  • the microcentrifugal tube includes a first hooking member 128 and a second hooking member 130 extending from the lid 118.
  • the first hooking member 128 and the second hooking member 130 may protrude from the lower surface of the lid 118.
  • the first and second hooking members 128 and 130 engage with the tubular body 102 to lock the lid 118 with the tubular body 102.
  • the hinge 120 has a through hole 132 disposed between the first end 122 and the second end 124 of the hinge 120.
  • the position of the first hooking member 128 substantially corresponds to the position of the flange 114 at the first edge portion 116 such that the first hooking member 128 engages the flange 114.
  • the position of the second hooking member 130 substantially corresponds to the position of the through hole 132 in the hinge 120 such that the second hooking member 130 at least partially passes through the through hole 132 and engages the hinge 120 at the through hole 132. In this way, the first edge portion 116 and the second portion 124 of the opening 110 can engage the lower surface of the lid 118 to seal the receiving cavity 104.
  • the hinge 120 may have a thicker region near the first end 122 and a thinner region near the second end 126.
  • the thicker region has a thickness greater than that of the thinner region.
  • the through hole 132 is formed in the thicker region. Therefore, the thicker region of the hinge 120 can be provided with mechanical strength sufficient to withstand a pulling force applied by the second hooking member 130 engaged therewith. Moreover, the thinner region of the hinge 120 still permits the bending of the hinge 120 and the associated movement of the lid 118.
  • the lid 118 can be securely locked with the tubular body 102 during centrifugation as the engagement of the first hinge member 102 with the flange 114 balances with the engagement of the second hinge member 130 at the through hole 132. This significantly improves the hermetical seal of the microcentrifugal tube 100 and prevents unwanted liquid leakage from the microcentrifugal tube 100.
  • the first hooking member 128 and the second hooking member 130 protrude from the lower surface of the lid 118 and bend towards a same direction.
  • the first and second hooking members both bend rightward as shown in Figure 5.
  • the second hooking member 130 engages the hinge 124 at a rim of the through hole 132, optionally away from the tubular body 102.
  • the first and second hooking members bending toward the same direction make the release of the lid 118 from the tubular body 102 much easier, as the two hooking members can both disengage from the tubular body 102 or the hinge 120 by pushing the lid 118 leftward with respect to the tubular body 102.
  • first hooking member 128 and the second hooking member 130 may bend towards each other, i. e. bend towards the center of the lid 118. Then the second hooking member 130 may engage the hinge 124 at the rim of the through hole 132 near the tubular body 102, or may engage the flange 114 if it is disposed around an entire circumference of the opening 110, i. e. the flange 114 may span from the first edge portion 116 to the second edge portion 124 and back to the first edge portion 116 to form the entire circumferential structure.
  • the second hooking member 130 may include two or more hooks that can be resiliently inserted into the through hole 132.
  • the first edge portion 116 and the second edge portion 124 of the opening 110 have an annular sealing layer (not shown) disposed thereon.
  • the lid 118 has a sealing member 134 protruding from its lower surface and being inserted into the tubular body 102 through the opening 110 when the lid 118 seals the opening 110.
  • the sealing member 134 may be sized to form interference fit with the opening 110 of the tubular body 102 when the lid 118 seals the opening 110 so that liquid can be tightly sealed within the receiving cavity 104.
  • the receiving cavity 104 may be shaped substantially as a cylinder, while the sealing member 134 may be shaped as a cylinder to hermetically seal the opening 110.
  • the opening 110 of the tubular body 102 has a lead-in surface 136.
  • the lead-in surface 136 helps the insertion of the sealing member 134 into the inside of the tubular body 102.
  • the lead-in surface 136 may be angled from about 30’ to about 5°, e. g. , 3° to a longitudinal axis of the tubular body 102.
  • the flange 114 may include one or more slots 138 at its lower surface.
  • one, two, three, four, six, eight or more slots may be formed at the lower surface of the flange 114.
  • the slots 138 can snug fit or mate with the supporting centrifuge mechanism, thereby avoiding the rotation of the microcentrifugal tube 100 relative to the supporting centrifugal mechanism.
  • the slots 138 may be uniformly and circumferentially spaced around the opening 110, which further improve the stability of the centrifugation of the microcentrifugal tube 100.
  • FIGs 2-5 illustrates horizontal cross sectional views of the microcentrifugal tube 100 in Figure 1 at different stages from when the lid does not seal the tubular body to when the lid seals the tubular body.
  • the operation of the microcentrifugal tube 100 will be further elaborated with references to Figures 2-5.
  • the hinge 120 is fully expanded and the lid 118 does not engage the tubular body 102. Then sample liquid may be added into the receiving cavity 104. Alternatively, the tubular body 102 may also be tilted to pour out the sample liquid contained therein.
  • the hinge 120 gradually bends to move the lid 118 counterclockwise towards the opening 110 of the tubular body 102. At the stage shown in Figure 4, the lid 118 gets much closer to the opening 110 such that the second hooking member 130 begins to pass through the through hole 132 of the hinge 120. Meanwhile, the sealing member 134 may also be in vicinity of the opening 110 such that the opening 110 is close to be sealed.
  • sample liquid may be further added into the receiving cavity 104, for example, by using a pipette with a small pipette tip or the like.
  • the lid 118 continues to move counterclockwise to move the second hooking member 126 downward to engage the hinge at the through hole 132.
  • the first hooking member 128 also moves to the position where it engages the flange 114.
  • the lid 118 is finally locked with the tubular body 102 via the first and second hooking members 128 and 130, thereby the microcentrifugal tube 100 is hermetically sealed to main the receiving cavity 104 free of contaminant.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Centrifugal Separators (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un tube microcentrifuge (100), qui comprend : un corps tubulaire (102) définissant une cavité de réception (104), le corps tubulaire (102) étant effilé au niveau d'une extrémité distale (108) et ayant une ouverture (110) au niveau d'une extrémité proximale (112), le corps tubulaire (102) ayant une bride (114) s'étendant d'une première partie bord (116) de l'ouverture (110); un couvercle (118) pour sceller l'ouverture (110) ayant un premier élément d'accrochage (128) et un second élément d'accrochage (130) s'étendant du couvercle (118); et une charnière (120) s'étendant, au niveau d'une première extrémité (122), d'une seconde partie bord (124) de l'ouverture (110) et fixée, au niveau d'une seconde extrémité (126), au couvercle (118). La charnière (120) comprend un trou traversant (132) disposé entre lesdites première et seconde extrémités (122, 126) de la charnière (120); lorsque le couvercle (118) ferme l'ouverture (110) du corps tubulaire (102), le premier élément d'accrochage (128) mettant en prise la bride (114) du corps tubulaire (102), et le second élément d'accrochage (130) mettant en prise la charnière (120) au niveau du trou traversant (132).
PCT/CN2014/086489 2013-09-13 2014-09-15 Tube microcentrifuge WO2015035949A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201480062387.1A CN106061612B (zh) 2013-09-13 2014-09-15 微量离心管

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201320567803.6 2013-09-13
CN201320567803 2013-09-13

Publications (1)

Publication Number Publication Date
WO2015035949A1 true WO2015035949A1 (fr) 2015-03-19

Family

ID=52665096

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2014/086489 WO2015035949A1 (fr) 2013-09-13 2014-09-15 Tube microcentrifuge

Country Status (2)

Country Link
CN (1) CN106061612B (fr)
WO (1) WO2015035949A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018193133A (ja) * 2017-05-17 2018-12-06 バイオティクス， インコーポレイテッド 人間工学的流体処理チューブ
USD881410S1 (en) 2018-01-19 2020-04-14 Biotix, Inc. Fluid handling tube
USD882113S1 (en) 2017-11-30 2020-04-21 Biotix, Inc. Fluid handling tube
CN111819002A (zh) * 2018-03-07 2020-10-23 埃佩多夫股份公司 用于实验室应用的由塑料制成的带盖器皿

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU185691U1 (ru) * 2018-10-10 2018-12-13 Общество с ограниченной ответственностью "ТЗК Техоснастка" Пробирка
CN211955507U (zh) * 2019-02-25 2020-11-17 上海快灵生物科技有限公司 一种生化反应试纸条管及试剂盒

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US5863791A (en) * 1996-07-11 1999-01-26 Eppendorf-Netheler-Hinz Gmbh Cover receptacle assembly
CN201596515U (zh) * 2010-01-15 2010-10-06 袁建华 一种易开盖微量离心管
CN202226864U (zh) * 2011-09-23 2012-05-23 安徽农业大学 微量离心管
US20120328490A1 (en) * 2010-12-23 2012-12-27 Eppendorf Ag Lidded Container
CN102861625A (zh) * 2012-09-26 2013-01-09 无锡耐思生物科技有限公司 微量离心管自动弹盖子结构

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US5354539A (en) * 1993-11-12 1994-10-11 Hovatter Kenneth R Microtube having press-to-seal and twist-to-lock closure cap
US6145688A (en) * 1996-07-17 2000-11-14 Smith; James C. Closure device for containers
DE102005058399A1 (de) * 2005-12-01 2007-06-14 Eppendorf Ag Deckelgefäß
CN202845041U (zh) * 2012-09-26 2013-04-03 无锡耐思生物科技有限公司 一种微量离心管自动弹盖子结构

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5863791A (en) * 1996-07-11 1999-01-26 Eppendorf-Netheler-Hinz Gmbh Cover receptacle assembly
CN201596515U (zh) * 2010-01-15 2010-10-06 袁建华 一种易开盖微量离心管
US20120328490A1 (en) * 2010-12-23 2012-12-27 Eppendorf Ag Lidded Container
CN202226864U (zh) * 2011-09-23 2012-05-23 安徽农业大学 微量离心管
CN102861625A (zh) * 2012-09-26 2013-01-09 无锡耐思生物科技有限公司 微量离心管自动弹盖子结构

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018193133A (ja) * 2017-05-17 2018-12-06 バイオティクス， インコーポレイテッド 人間工学的流体処理チューブ
US10449535B2 (en) 2017-05-17 2019-10-22 Biotix, Inc. Ergonomic fluid handling tubes
US10562023B2 (en) 2017-05-17 2020-02-18 Biotix, Inc. Ergonomic fluid handling tubes
JP2021104861A (ja) * 2017-05-17 2021-07-26 バイオティクス， インコーポレイテッド 人間工学的流体処理チューブ
US11433391B2 (en) 2017-05-17 2022-09-06 Biotix, Inc. Ergonomic fluid handling tubes
JP7300812B2 (ja) 2017-05-17 2023-06-30 バイオティクス， インコーポレイテッド 人間工学的流体処理チューブ
USD882113S1 (en) 2017-11-30 2020-04-21 Biotix, Inc. Fluid handling tube
USD881410S1 (en) 2018-01-19 2020-04-14 Biotix, Inc. Fluid handling tube
CN111819002A (zh) * 2018-03-07 2020-10-23 埃佩多夫股份公司 用于实验室应用的由塑料制成的带盖器皿
US11896976B2 (en) 2018-03-07 2024-02-13 Eppendorf Ag Lidded vessel assembly for laboratory use

Also Published As

Publication number Publication date
CN106061612A (zh) 2016-10-26
CN106061612B (zh) 2017-11-17

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