WO2014127774A1 - Récipient pour accueillir des milieux nutritifs - Google Patents

Récipient pour accueillir des milieux nutritifs Download PDF

Info

Publication number
WO2014127774A1
WO2014127774A1 PCT/DE2014/200027 DE2014200027W WO2014127774A1 WO 2014127774 A1 WO2014127774 A1 WO 2014127774A1 DE 2014200027 W DE2014200027 W DE 2014200027W WO 2014127774 A1 WO2014127774 A1 WO 2014127774A1
Authority
WO
WIPO (PCT)
Prior art keywords
bottom plate
wall
base body
container according
nutrient medium
Prior art date
Application number
PCT/DE2014/200027
Other languages
German (de)
English (en)
Inventor
Rolf Mueller
Original Assignee
Pharmamedia Dr. Mueller Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmamedia Dr. Mueller Gmbh filed Critical Pharmamedia Dr. Mueller Gmbh
Priority to EP14709539.2A priority Critical patent/EP2812423A1/fr
Publication of WO2014127774A1 publication Critical patent/WO2014127774A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/54—Constructional details, e.g. recesses, hinges hand portable
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object

Definitions

  • the invention relates to a container for holding nutrient media, in particular petri dish, contact plate or sedimentation plate, for the cultivation of microorganisms, cell cultures, bacteria, etc.
  • Containers for receiving nutrient media are well known in practice. For example, reference is made to DE 10 2007 027 273 A1. From this document, a so-called contact plate or Abklatschplatte is known, namely a Petri dish, which is filled with solidified nutrient medium. To handle the Petri dish in the sense of a contact plate, the shell is filled so that the nutrient medium, regularly agar, protrudes at least slightly beyond the peripheral side wall of the shell. To clot, the container is gripped on the peripheral wall of the shell and the supernatant surface of the nutrient medium is pressed onto the surface to be "clipped.” Contaminants, especially bacteria, adhere to the surface of the solidified nutrient medium, providing an ideal breeding ground for bacterial cultures.
  • the shell After wiping off the contaminated surface of the nutrient medium is covered or the shell is closed with a lid which is at least slightly spaced from the surface of the nutrient medium, with the bacteria adhering there. Between the inner wall of the lid and the surface of the nutrient medium remains a sufficiently large space, so that in the prevailing atmosphere, the cell cultures can develop. There are aerobic and anaerobic embodiments of such contact plates known, as needed and objective with respect to the bacterial cultures. In other words, the lid can air or gas-tight or ventilated attach to the shell body.
  • the present invention is based on the object, a container of the generic type to design and further develop that eliminates the above problems, but at least are significantly reduced. Specifically, condensation in the area above the nutrient medium - before use - should be avoided.
  • the filling of the container should be possible with simple means in a very short time.
  • the handling both during filling and in the application should be as simple as possible.
  • the container for receiving nutrient media in particular petri dish, contact plate or sedimentation, for culturing microorganisms, bacteria, etc., comprises a base body with circumferential wall and a bottom plate with a base and peripheral wall.
  • the main body and the bottom plate form a shell.
  • For filling the shell with nutrient medium for storage and transport of the main body is inserted into the peripheral wall of the bottom plate, wherein the wall of the body at least slightly to the base of the bottom plate is spaced.
  • the base body can be inserted or inserted into the base plate, such that when pouring in the nutrient medium, it protrudes from the peripheral wall of the base body.
  • the nutrient medium is poured into the base body from the back and terminates with the inner wall of the bottom plate. After the pouring of the nutrient medium, any space between the main body and the bottom plate is filled, so that the previously available air space on the surface of the supernatant medium is eliminated. A condensation is therefore excluded there.
  • the exclusion of condensation is of particular importance in order to avoid soiling, especially smear infections. In particular, it is about contamination of user equipment.
  • the container according to the invention has the advantage that when pouring the nutrient medium no air bubbles on or on the contact surface arise because these, namely, if present, during filling and immediately afterwards in the nutrient medium rise up, namely from the contact surface (Abklatsch components ) remove. Since any bubbling does not present any problems insofar as the bubbles do not form on the surface used for culturing, the filling with nutrient medium can be accelerated once again. This is of special importance.
  • a closure is attached after filling the body with nutrient medium, so that the container is closed on the Abklatschseite by the bottom plate and on the Abklatschseite facing away from the filling through the closure.
  • the container is transported as a contact plate or Sedimentationsplatte and stored or provided for use.
  • the container according to the invention implies a very special method, namely a method for filling such a container, after which by the base body and the bottom plate formed shell is filled from the back with liquid nutrient medium, and wherein a distance of the peripheral wall of the base body relative to the base of the bottom plate ensures a supernatant of the nutrient medium on the circumferential wall of the body out. At the same time the usual air space between the surface of the nutrient medium and the inner wall of a lid is avoided. Condensation can therefore not take place there.
  • the container After the back filling of the container, namely from the contact surface of the culture medium side facing away, the container is closed on the back, including a special closure is used, which can also be equipped with sealing film.
  • the spacing of the wall of the main body from the base surface of the bottom plate is realized by a step formed near or at the base surface, preferably inwardly encircling step, groove or the like.
  • the base body according to the dimensions of the step, fillet or the like. Not a total plug in the wall of the bottom plate, but with a small distance to the base of the bottom plate. This distance ensures when pouring the nutrient medium required for Abklatschen supernatant of the nutrient medium.
  • the step, groove or the like can be performed circumferentially. It is also conceivable that this measure is provided for example at three or more locations, namely to define the distance of the circumferential wall of the base body relative to the base surface of the bottom plate.
  • the step, groove or the like may have a height in the range of 0.1 mm to 5 mm, so that in fact the nutrient medium protrudes sufficiently beyond the edge region of the main body.
  • the solidified nutrient medium is at least slightly exposed to the circumferential wall in accordance with the spacing of the wall of the base body to the bottom plate, resulting in the usability of the filled container as a contact plate.
  • the achieved It is necessary to bring the nutrient medium into contact with the surfaces to be tested or contaminated.
  • a contact plate is a "crowded" petri dish, namely a Petri dish filled with nutrient medium, in which the nutrient medium protrudes beyond the edge area of the dish.
  • the main body forming the shell has, on the side facing away from the contact surface, namely on the filling side, a closure in the sense of a removable cover.
  • This cover is attached after filling the body or the shell for closing the container.
  • This may be, for example, a film laid over the edge, in particular additionally glued thereto, or the like.
  • the cover is designed as a lid with circumferential wall, which encompasses the base body.
  • the container When filled, the container consists of the base body, a contact surface covering the bottom plate and a filler-side lid.
  • On the opposite side of the main body or the shell is closed on the back by a lid.
  • the wall of the lid is designed in a particularly sophisticated manner and dimensioned so that it is after removal of the bottom plate instead of this on the wall of the shell, this cross-over, can be placed and thereby for spaced closing of the shell, forming a space above the nutrient solution, serves.
  • This space is necessary for the cultivation of microorganisms, because there is a certain airspace or a certain atmosphere for To be provided for transport and storage of the container with filled culture medium, before use, to be avoided.
  • the wall of the bottom plate is designed and dimensioned such that the bottom plate after removal of the cover instead of this on the wall of the shell, this cross-over, can be placed.
  • the cover and the bottom plate due to their specific design, can optionally be attached to both sides of the body, wherein for providing the contact plate with nutrient medium, the bottom plate sits directly on the contact surface of the nutrient medium, namely due to the special filling according to the foregoing ,
  • the container can also be used as a sedimentation plate, regardless of the above.
  • the base body on the outside of its wall may have a stop for the wall of the bottom plate and / or for the wall of the lid, whereby the attachment of the bottom plate and lid is limited or defined. It should be taken into account the situation that the circumferential wall of the bottom plate and the lid differently are executed high, namely the different spacing from the edge of the container.
  • the main body has either as a common stop for the bottom plate and the lid or in the middle between the stops for the bottom plate and the lid a preferably circumferential, projecting from the outer wall handling area for gripping the body.
  • This handling area can be designed as a circumferential ring, which can be an integral part of the outer wall of the base body. It is also conceivable that a ring area is pushed onto the base body. This can be a ring region made of the same material as the main body or, for example, rubber or the like.
  • the basic body preferably comprises anchoring means extending through the interior of the basic body in a central or lower area. These anchoring means are surrounded during filling with nutrient medium and serve to hold the poured nutrient medium, on the one hand when lifting or removing the bottom plate and on the other hand in the process of Abklatschens.
  • the anchoring means favor the "holding" of the nutrient medium in the main body or in the shell formed by the main body.
  • the anchoring means may be struts or a lattice-like structure, so that unintentional slipping out of the nutrient medium from the main body is effective is avoided.
  • the bottom plate forms the shell together with the base body.
  • the base body When pouring the nutrient medium this contacts the inner wall of the bottom plate. Due to adhesion, it may take a certain amount of effort to lift the base plate from the body or deduct.
  • the removal of the base plate from the base body favoring measures can be provided, for example to the effect that by turning, preferably by sliding along a ramp, the bottom plate of the main body and thus of the solidified nutrient medium can be lifted or is removable.
  • the lifting of the bottom plate can also be favored in that an at least slight tilting of the bottom plate relative to the base body is possible, whereby the inner wall of the bottom plate can be lifted off the surface of the nutrient medium and thus removed.
  • bottom plate and / or the cover or the lid can be connected by any connection mechanisms with the body / can, for example by Stellerp-, screw or bayonet connection. It is also conceivable that the individual components are locked against each other or with each other.
  • Fig. 1 in a schematic side view, cut, an embodiment of a container according to the invention with filled nutrient medium and the rear side lid and
  • FIG. 2 is a schematic plan view of an insight into the interior of the main body, which has in a central area an integral grid structure for anchoring the poured-in nutrient medium.
  • Figure 1 shows a schematic view of an embodiment of a container according to the invention for receiving nutrient media, wherein it is in concrete terms a contact plate, which also as Use sedimentation plate.
  • the container is used for the cultivation of microorganisms, bacteria, etc.
  • the container shown in Figure 1 comprises a base body 1 and a bottom plate 2, wherein the base body 1 and the bottom plate 2 together form a shell, namely for filling with nutrient medium 3.
  • the nutrient medium 3 may be, for example, agar.
  • the base body 1 consists essentially of a peripheral wall 4.
  • the bottom plate 2 consists of a base 5 and a peripheral wall 6, so that the bottom plate 2 with respect to the base body 1 in the sense of a closure, lid 9 or the like. Is executed.
  • FIG. 1 clearly shows that the base body 1 is set or plugged into the base plate 2 or its circumferential wall 6.
  • a kind of spacer 7 in the form of a step, fillet or the like. Ensures that the wall 4 of the body 1 is positioned at a small distance to the base 5 of the bottom plate 2, so that when filling the nutrient medium 3 on the base 5 of the bottom plate 2 comes to rest and thereby protrudes at least slightly beyond the wall 4 of the base body 1. As a result, the function is guaranteed as a contact plate.
  • the bottom plate 2 On the side facing away from the nutrient medium 3, the bottom plate 2 has feet 8 or the like, which serve for positioning or parking the container.
  • a stop 1 1 defines the degree of Aufsteckens both the bottom plate 2 and the lid 10 on the outer wall 4 of the main body.
  • the bottom plate 2 is removed so that the solidified nutrient medium 3 is freely accessible, namely at least slightly over the wall 4 of the body. 1 protrudes.
  • the use as a contact plate is ideally possible.
  • the lid 10 which can be taken from the side facing away from the nutrient medium 3, from the filling side of the main body 1, is ideally suited. Due to the higher-trained wall 12 and on the basis of the stop 1 1, the lid 10 can be attached with considerable spacing to the surface of the nutrient medium 3 from the Abklatschseite on the base body 1, as indicated in Figure 1.
  • the then existing space 13 between the surface of the nutrient medium 3 and the inner wall of the lid 10 ensures a cultivation of microorganisms located on the surface, cell cultures, bacteria, etc.
  • the bottom plate 2, which has originally covered the surface of the nutrient medium 3 without gap can on the back, ie on the filling side of the base body 1, are plugged onto the wall 4.
  • the container can be used for culturing the microorganisms or the like., For example, under tempered conditions.
  • FIG. 1 further shows that the stop 1 1 can be part of a handling area 14, for example in the form of a circumferential annular area on the wall 4 of the base body 1.
  • This handling area 14 serves to grasp the base body 1 and thus to provide ideal handling.
  • anchoring means 15 are provided, which extend approximately centrally or in a lower region through the base body 1 therethrough.
  • the anchoring means 15 serve to hold the infused into the base body 1 nutrient medium 3, so that it can not slide out of the main body 1 during handling of the container. A secure holding of the nutrient medium 3 within the body 1 is thus ensured.
  • FIG. 2 shows a top view of the embodiment of the anchoring means 15 in the sense of a grid structure 16, this being an advantageous embodiment of the anchoring means 15.
  • the individual components of the container can be made of plastic, preferably by injection molding technology. It is advisable to use an at least partially transparent plastic material. Both the main body 1 and the bottom plate 2 and the lid 10 may be directly labeled or be labeled in the course of handling. It is also conceivable that labels are applied to local areas, namely to document the location of the sample taken.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un récipient pour accueillir des milieux nutritifs (3), en particulier une cuve de Petri, une plaque de contact ou une plaque de sédimentation, pour la culture de micro-organismes, de cellules, de bactéries, etc. Ledit récipient comprend un corps de base (1) avec une paroi périphérique (4) et une plaque de fond (2) comprenant une surface de base (1) et une paroi périphérique. Le corps de base (1) et la plaque de fond (3) forment une cuve. La cuve sert au remplissage avec une solution nutritive, au stockage et au transport. Le corps de base (1) est introduit ou enfiché dans la paroi périphérique de la plaque de fond (6), avec au moins un écartement modéré par rapport à la surface de base de la plaque de fond (5). Du côté détourné de la plaque de fond (2), le corps de base comporte un verrouillage que l'on met en place après le remplissage du corps de base (1) en le milieu nutritif (3) et qui ferme le corps de base (1) avec le milieu nutritif qui s'y trouve.
PCT/DE2014/200027 2013-02-21 2014-01-24 Récipient pour accueillir des milieux nutritifs WO2014127774A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP14709539.2A EP2812423A1 (fr) 2013-02-21 2014-01-24 Récipient pour accueillir des milieux nutritifs

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE102013202859 2013-02-21
DE102013202859.8 2013-02-21
DE201310204148 DE102013204148A1 (de) 2013-02-21 2013-03-11 Behältnis zur Aufnahme von Nährmedien
DE102013204148.9 2013-03-11

Publications (1)

Publication Number Publication Date
WO2014127774A1 true WO2014127774A1 (fr) 2014-08-28

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ID=51353108

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2014/200027 WO2014127774A1 (fr) 2013-02-21 2014-01-24 Récipient pour accueillir des milieux nutritifs

Country Status (3)

Country Link
EP (1) EP2812423A1 (fr)
DE (1) DE102013204148A1 (fr)
WO (1) WO2014127774A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107815395A (zh) * 2017-12-22 2018-03-20 福建上源生物科学技术有限公司 培养皿收纳容器及其使用方法
US11680236B2 (en) 2017-11-30 2023-06-20 Corning Incorporated Thin, uniform, stackable petri dish

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE479304C (de) * 1929-07-13 M Wafik Zaher Dr Vorrichtung zur Zuechtung von Mikroorganismen unter Sauerstoffabschluss
US4598050A (en) * 1983-12-02 1986-07-01 Brown Lewis R Culture plate for surfaces
WO1993024608A1 (fr) * 1992-05-22 1993-12-09 Seaborn, George, Stephen Dispositif de culture permettant d'echantillonner et/ou de compter des microorganismes
DE4406725A1 (de) 1994-03-02 1995-09-07 Heipha Gmbh Behältnis
EP0952210A1 (fr) * 1998-04-24 1999-10-27 Millipore S.A. Cassette ainsi que procédé et appareil d'analyse de l'air l'utilisant
US6602704B1 (en) 2002-06-24 2003-08-05 Biomerieux, Inc. Sample contact plate with latchable cover
DE102007027273A1 (de) 2007-05-24 2008-11-27 Heipha Gmbh Behältnis zur Aufnahme von Nährmedien
WO2008149914A2 (fr) * 2007-05-30 2008-12-11 Nikon Corporation Bac d'incubation
US20110174820A1 (en) * 2010-01-18 2011-07-21 Becton, Dickinson And Company Container Assembly
WO2012069106A1 (fr) * 2010-11-23 2012-05-31 Sartorius Stedim Biotech Gmbh Fermeture pour récipient à produit alimentaire

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2605330B1 (fr) * 1986-10-20 1989-01-20 Millipore Sa Recipient destine a recevoir un ou plusieurs milieux de culture pour microorganismes.

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE479304C (de) * 1929-07-13 M Wafik Zaher Dr Vorrichtung zur Zuechtung von Mikroorganismen unter Sauerstoffabschluss
US4598050A (en) * 1983-12-02 1986-07-01 Brown Lewis R Culture plate for surfaces
WO1993024608A1 (fr) * 1992-05-22 1993-12-09 Seaborn, George, Stephen Dispositif de culture permettant d'echantillonner et/ou de compter des microorganismes
DE4406725A1 (de) 1994-03-02 1995-09-07 Heipha Gmbh Behältnis
EP0952210A1 (fr) * 1998-04-24 1999-10-27 Millipore S.A. Cassette ainsi que procédé et appareil d'analyse de l'air l'utilisant
US6602704B1 (en) 2002-06-24 2003-08-05 Biomerieux, Inc. Sample contact plate with latchable cover
DE102007027273A1 (de) 2007-05-24 2008-11-27 Heipha Gmbh Behältnis zur Aufnahme von Nährmedien
WO2008149914A2 (fr) * 2007-05-30 2008-12-11 Nikon Corporation Bac d'incubation
US20110174820A1 (en) * 2010-01-18 2011-07-21 Becton, Dickinson And Company Container Assembly
WO2012069106A1 (fr) * 2010-11-23 2012-05-31 Sartorius Stedim Biotech Gmbh Fermeture pour récipient à produit alimentaire

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2812423A1 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11680236B2 (en) 2017-11-30 2023-06-20 Corning Incorporated Thin, uniform, stackable petri dish
CN107815395A (zh) * 2017-12-22 2018-03-20 福建上源生物科学技术有限公司 培养皿收纳容器及其使用方法

Also Published As

Publication number Publication date
EP2812423A1 (fr) 2014-12-17
DE102013204148A1 (de) 2014-09-04

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