WO2014096389A1 - Méthode d'identification de cellules produisant des anticorps - Google Patents

Méthode d'identification de cellules produisant des anticorps Download PDF

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Publication number
WO2014096389A1
WO2014096389A1 PCT/EP2013/077757 EP2013077757W WO2014096389A1 WO 2014096389 A1 WO2014096389 A1 WO 2014096389A1 EP 2013077757 W EP2013077757 W EP 2013077757W WO 2014096389 A1 WO2014096389 A1 WO 2014096389A1
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WO
WIPO (PCT)
Prior art keywords
cells
antigen
population
cell
labels
Prior art date
Application number
PCT/EP2013/077757
Other languages
English (en)
Inventor
Daniel John Lightwood
Stephen Edward Rapecki
Dale Owen STARKIE
Original Assignee
Ucb Pharma S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb Pharma S.A. filed Critical Ucb Pharma S.A.
Publication of WO2014096389A1 publication Critical patent/WO2014096389A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins

Definitions

  • the IgG marker for use in step a), is a mixture of two or more IgG markers for different isotypes.
  • the IgG marker is a mixture of anti-IgGl , anti-IgG2A and anti-IgG2B.
  • the method according to the present disclosure further comprises the step of identifying a B cell in the population employing a functional assay.
  • Identification of antibody secreting cells may be identified using a functional screening assay, for example a binding assay or measuring a functional effect such as neutralisation of a given activity.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne une méthode d'identification d'une cellule produisant un anticorps spécifique d'un antigène cible comprenant les étapes suivantes : a) fournir une population de lymphocytes B produisant un anticorps, ladite population ayant été sélectionnée en fonction d'au moins un marqueur de lymphocyte B et/ou au moins un marqueur sélectionné dans le groupe constitué d'un marqueur IgG, IgE et IgA et d'au moins deux marqueurs de « dé-sélection » (non présents sur le lymphocyte B cible), b) incuber la population de lymphocytes B de l'étape a) avec ledit antigène cible et au moins deux indicateurs distincts dudit antigène de façon que l'antigène soit dans une forme indiquée avec un premier indicateur et/ou un deuxième indicateur, c) sélectionner dans la population de cellules les cellules capables de produire des anticorps spécifiques de l'antigène cible, les cellules intéressantes étant identifiées grâce à au moins un de chacun desdits deux indicateurs, et d) sélectionner la population à multiples indications d'antigènes des cellules identifiées dans l'étape c), et e) facultativement, séparer la population des cellules sélectionnées dans l'étape d) en cellules uniques.
PCT/EP2013/077757 2012-12-21 2013-12-20 Méthode d'identification de cellules produisant des anticorps WO2014096389A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1223316.9 2012-12-21
GB201223316A GB201223316D0 (en) 2012-12-21 2012-12-21 Method

Publications (1)

Publication Number Publication Date
WO2014096389A1 true WO2014096389A1 (fr) 2014-06-26

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2013/077757 WO2014096389A1 (fr) 2012-12-21 2013-12-20 Méthode d'identification de cellules produisant des anticorps

Country Status (2)

Country Link
GB (1) GB201223316D0 (fr)
WO (1) WO2014096389A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108732082A (zh) * 2018-06-07 2018-11-02 北京唯公医疗技术有限公司 细胞分群方法
JP2021120679A (ja) * 2017-01-18 2021-08-19 エッセン インストラメンツ，インコーポレイテッド ディー／ビー／エー エッセン バイオサイエンス，インコーポレイテッド 生物学的サンプルから免疫グロブリンγ（ＩｇＧ）抗体アイソタイプの濃度を決定するための方法および試薬

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018068A1 (fr) * 1992-03-09 1993-09-16 Tanox Biosystems, Inc. Procedes permettant de selectionner des lymphocytes b peu communs specifiques d'un antigene
WO2006072803A2 (fr) * 2005-01-10 2006-07-13 Medical Research Council Anticorps
WO2013030799A1 (fr) * 2011-09-02 2013-03-07 Novartis Ag. Dosage de cellule b

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018068A1 (fr) * 1992-03-09 1993-09-16 Tanox Biosystems, Inc. Procedes permettant de selectionner des lymphocytes b peu communs specifiques d'un antigene
WO2006072803A2 (fr) * 2005-01-10 2006-07-13 Medical Research Council Anticorps
WO2013030799A1 (fr) * 2011-09-02 2013-03-07 Novartis Ag. Dosage de cellule b

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TOWNSEND S E ET AL: "Single epitope multiple staining to detect ultralow frequency B cells", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 249, no. 1-2, 1 March 2001 (2001-03-01), pages 137 - 146, XP004317478, ISSN: 0022-1759, DOI: 10.1016/S0022-1759(00)00352-5 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021120679A (ja) * 2017-01-18 2021-08-19 エッセン インストラメンツ，インコーポレイテッド ディー／ビー／エー エッセン バイオサイエンス，インコーポレイテッド 生物学的サンプルから免疫グロブリンγ（ＩｇＧ）抗体アイソタイプの濃度を決定するための方法および試薬
JP7170782B2 (ja) 2017-01-18 2022-11-14 ザルトリウス バイオアナリティカル インストゥルメンツ， インコーポレイテッド 生物学的サンプルから免疫グロブリンγ（ＩｇＧ）抗体アイソタイプの濃度を決定するための方法および試薬
US11733237B2 (en) 2017-01-18 2023-08-22 Sartorius Bioanalytical Instruments, Inc. Methods and reagents for determining immunoglobulin gamma (IgG) antibody isotype concentration from biological samples
CN108732082A (zh) * 2018-06-07 2018-11-02 北京唯公医疗技术有限公司 细胞分群方法

Also Published As

Publication number Publication date
GB201223316D0 (en) 2013-02-06

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