WO2014045759A1 - Dispositif d'affichage d'image, procédé, programme et système de microscope - Google Patents

Dispositif d'affichage d'image, procédé, programme et système de microscope Download PDF

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Publication number
WO2014045759A1
WO2014045759A1 PCT/JP2013/071784 JP2013071784W WO2014045759A1 WO 2014045759 A1 WO2014045759 A1 WO 2014045759A1 JP 2013071784 W JP2013071784 W JP 2013071784W WO 2014045759 A1 WO2014045759 A1 WO 2014045759A1
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Prior art keywords
information
image display
unit
display device
pathological specimen
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PCT/JP2013/071784
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English (en)
Japanese (ja)
Inventor
山根　健治
徹 見留
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ソニー株式会社
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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides

Definitions

  • the present technology relates to an image display device, an image display method, a program, and a microscope system that display an image captured by a microscope. More specifically, the present invention relates to a technique for selecting and displaying specific image data from a plurality of microscope observation image data.
  • a virtual microscope system using this technology is particularly attracting attention for tissue / cell examination applications in pathological diagnosis because the user can display an arbitrary position at an arbitrary magnification for the obtained microscope observation image. ing.
  • specimen information provided to a user such as a pathologist
  • a microscopic observation image examples include the name of the person who collected it, the date and time of collection, the type of staining, and the number of the specimen.
  • a bright-field image and a dark-field image are captured for one specimen, a sample is detected from the bright-field image, and a label in which the specimen information is described is detected from the dark-field image, and these are associated and output.
  • a microscope has been proposed (see Patent Document 2).
  • sample information may be recorded on a barcode or RFID (radio frequency identification) in addition to being described on the label (see, for example, Patent Document 3).
  • a barcode or RFID radio frequency identification
  • the information stored in a barcode or RFID is read by a reader, and the information is associated with the captured microscope observation image.
  • a search is performed by specifying a case, a specimen number, patient information, or the like, and a plurality of images obtained.
  • the target image must be further selected from among them.
  • the operation for selecting and displaying a specific microscope observation image from the image data stored in the server is complicated.
  • tissue and cell examinations when a plurality of pathological specimens are prepared and observed for a single patient, or because a plurality of images may be acquired by changing conditions for one pathological specimen, It takes time to identify.
  • the main object of the present disclosure is to provide an image display device, an image display method, a program, and a microscope system that can easily and reliably select and display a specific microscope observation image.
  • An image display device includes a sample recognition unit that reads identification information of a pathological specimen to be browsed, and sample information that transmits the identification information to an information processing apparatus and acquires information on the pathological specimen from the information processing apparatus And an acquisition unit.
  • the information on the pathological specimen may be microscope observation image data obtained by imaging the pathological specimen or access information to the microscope observation image data.
  • an access information storage unit that stores the access information may be provided, and an image data acquisition unit that accesses the information processing apparatus based on the access information and acquires the microscope observation image data is provided. It may be.
  • an information input unit for a user to input information related to the microscope observation image data can be provided.
  • the sample recognition unit is, for example, a barcode or RF tag reader.
  • the identification information may be attached to a slide glass in which the pathological specimen is enclosed. Furthermore, you may provide the display part which can browse the microscope observation image which imaged the said pathological specimen.
  • An image display method includes a sample recognition step of reading identification information of a pathological specimen to be browsed by a sample recognition unit, and transmitting the identification information to an information processing device by a sample information acquisition unit. And a sample information acquisition step of acquiring information on the pathological specimen.
  • a program transmits a sample recognition function for reading identification information of a pathological specimen to be browsed to an image display device including at least a sample recognition unit and a sample information acquisition unit, and the identification information to an information processing device. And a sample information acquisition function for acquiring information on the pathological specimen from the information processing apparatus.
  • a microscope system includes a sample recognition unit that reads identification information of a pathological specimen to be browsed, a sample information acquisition unit that transmits the identification information to an information processing apparatus, and acquires information on the pathological specimen from the information processing apparatus And an information storage unit that stores information on the pathological specimen, and an information extraction unit that extracts information to be provided to the image display unit from the information storage unit in response to a request from the image display unit
  • An information processing apparatus including at least a unit.
  • the information storage unit of the information processing apparatus stores a microscope observation image of the pathological specimen imaged by the microscope apparatus, and identification information of the pathological specimen associated with the microscope observation image
  • the information extraction unit may extract microscope observation image data associated with the identification information or access information to the microscope observation image data based on the identification information transmitted from the image display device.
  • the identification information of the pathological specimen is transmitted to the information processing apparatus, and the information regarding the pathological specimen is acquired, so that a specific microscope observation image can be easily and reliably selected and displayed. it can.
  • FIG. 1 It is a figure showing an outline of a microscope system of a 1st embodiment of this indication.
  • A is a figure which shows an example of a pathological sample
  • FIG. 6 is a flowchart showing the operation of the image display device 4 when requesting access information from the server 3. It is a flowchart figure which shows operation
  • FIG. It is a flowchart figure which shows the operation
  • FIG. 6 is a flowchart showing the operation of the image display device 4 when requesting access information from the server 3. It is a flowchart figure which shows operation
  • FIG. It is a flowchart figure which shows the operation
  • First embodiment an example in which a viewer acquires access information from a server based on identification information
  • Second embodiment an example in which a viewer acquires microscope observation image data from a server based on identification information
  • FIG. 1 is a diagram showing an outline of the microscope system of the present embodiment.
  • the microscope system of the present embodiment is for observing images of various prepared specimens taken at a high magnification.
  • the microscope system includes a digital microscope 1, an information processing device 2, an image display device 4, and the like.
  • the information processing device 2 and the image display device 4 may be directly connected or may be connected via the network 5. Further, the information processing apparatus 2 and the image display apparatus 4 may be connected to the server 3 via the network 5 so as to be able to communicate with each other. Further, in the configuration shown in FIG. 1, only one image display device 4 is shown, but two or more image display devices 4 may be connected, or the information processing device 2 may connect the image display device 4. You may also serve.
  • the microscope system of the present embodiment can be applied to various fields such as medical treatment, pathology, living organisms, and materials.
  • the observation target is a living organ, tissue, cell, etc.
  • These sections are enclosed in the preparation specimens.
  • the prepared specimen is a pathological specimen
  • the user (image viewer) of the image display device 4 is a doctor or the like, and performs a pathological diagnosis based on the displayed image.
  • a case where the preparation specimen is a pathological specimen will be described as an example.
  • the digital microscope 1 includes a light source, an objective lens, an image sensor, a stage, and the like.
  • the pathological specimen placed on the stage is irradiated with a predetermined illumination light, and the light transmitted through the observation object or the observation object Imaging light emitted from an object.
  • FIG. 2 is a block diagram illustrating a configuration example of the digital microscope 1. As shown in FIG. 2, the digital microscope 1 provided in the microscope system of the present embodiment is provided with an entire image capturing unit 11 and a partial image capturing unit 12.
  • the whole image capturing unit 11 includes a light source, a low-magnification objective lens, a low-resolution image sensor, and the like, and images the entire observation target region of the pathological specimen placed on the stage at a low magnification and a low resolution.
  • the partial image capturing unit 12 includes a light source, a high-magnification objective lens, a high-resolution image sensor, and the like, and a part of the observation target region of the pathological specimen placed on the stage is Shoot at high resolution. That is, the digital microscope 1 captures a low-resolution whole image (thumbnail image) and a partial image (slide image) having a higher resolution than the whole image.
  • the digital microscope 1 is provided with an imaging device controller 13 that controls imaging processing by the whole image imaging unit 11 and the partial image imaging unit 12, an input / output interface unit 14 that is connected to the information processing device 2, and the like. May be.
  • an imaging device controller 13 that controls imaging processing by the whole image imaging unit 11 and the partial image imaging unit 12
  • an input / output interface unit 14 that is connected to the information processing device 2, and the like. May be.
  • the input / output interface unit 14 it is possible to input a control command from the information processing device 2 and output each image data captured by the entire image capturing unit 11 and the partial image capturing unit 12 to the information processing device 2. Become.
  • FIG. 3 is a block diagram illustrating a configuration example of the information processing apparatus 2.
  • the information processing apparatus 2 includes an imaging control unit 21, an identification information recognition unit 22, an image processing unit 23, a CPU (Central Processing Unit) 24, a memory 25, a hard disk 26, and input / output interface units 27 and 28. Etc.
  • Imaging control unit 21 controls driving of the digital microscope 1 when capturing a partial image (slide image), an entire image (thumbnail image), or the like. For example, the imaging control unit 21 analyzes an entire image (thumbnail image) obtained by imaging the entire observation target region, and creates an order (imaging sequence) in which the digital microscope 1 captures partial images.
  • the identification information recognition unit 22 acquires the identification information from the pathological specimen to be imaged.
  • the identification information acquired in the identification information recognition unit 22 may be any information as long as the captured microscope observation image can be identified, and examples include a case number, a slide name, a patient name, a staining name, and a slide number. 1 type (s) or 2 or more types can be acquired.
  • the acquisition method is not particularly limited.
  • the identification information when the identification information is described on a label attached to a slide glass in which a pathological specimen is enclosed, the information can be read by image recognition. .
  • the identification information When the identification information is recorded on a barcode or RFID (radio frequency identification), the information can be read and acquired by a device that can read the identification information.
  • the identification information acquired by the identification information recognition unit 22 is stored in the hard disk 26 and / or the sample information storage unit 313 of the server 3.
  • the image processing unit 23 combines a plurality of partial images (slide images) captured by the digital microscope 1 to synthesize a wide-field microscope observation image with high magnification and high resolution. Specifically, matching processing is performed on partial images (slide images) to be connected, and partial images (slide images) are connected (stitched) based on the result.
  • the synthesized image (wide-field microscope observation image) synthesized by the image processing unit 23 is stored in the hard disk 26 and / or the image data storage unit 312 of the server 3.
  • the CPU 24 comprehensively controls each unit provided in the information processing apparatus 2, and executes a program that comprehensively controls the above-described imaging control unit 21, identification information recognition unit 22, image processing unit 23, and the like, for example. To do.
  • the CPU 24 can also perform arithmetic processing executed by each unit of the information processing apparatus 2, perform image encoding, pattern matching processing of partial images executed by the image processing unit 23, and the like.
  • the memory 25 is used as a work area of the CPU 24, and temporarily stores a partial image (slide image), an entire image (thumbnail image), etc. that are photographed by the digital microscope 1 and input from the input / output interface unit 27.
  • Hard disk 26 In the hard disk 26, for example, a composite image (microscopic observation image) synthesized by the image processing unit 23, pathological specimen identification information acquired by the identification information recognition unit 22, and the like are stored. Some or all of these data can also be stored in the server 3 on the network 5.
  • the information processing apparatus 2 may be provided with a connection interface 28 with the server 3 so that they can communicate with each other via the network 5.
  • Such a computer program may be stored in a recording medium such as a magnetic disk, an optical disk, a magneto-optical disk, or a flash memory, and can be distributed via a network.
  • FIG. 4 is a block diagram illustrating a configuration example of the server 3. As shown in FIG. 4, the server 3 is connected to the information processing device 2 and the image display device 4 via the network 5. For example, the information storage unit 31, the information extraction unit 32, the CPU 33, the input / output interface unit 34, 35 or the like.
  • the information storage unit 31 includes a reception unit 311, an image data storage unit 312, and a specimen information storage unit 313, and receives image data of a composite image (microscopic observation image) uploaded from the information processing apparatus 2 and identification information thereof.
  • the accepting unit 311 associates the uploaded image data with the identification information, and generates information (access information) for accessing the image data.
  • this access information for example, the URL of the access destination can be cited.
  • the image data storage unit 312 stores image data of a composite image (microscope observation image).
  • the specimen information storage unit 313 stores the access information generated by the reception unit 311 together with the identification information of the image data stored in the image data storage unit 312.
  • Information extraction unit 32 In response to a request from the image display device 4, the information extraction unit 32 extracts access information to the image data of the microscopic observation image to be browsed from the information storage unit 31 and transmits the information to the image display device 4.
  • the CPU 33 controls each unit provided in the server 3 in an integrated manner, and executes, for example, a program that comprehensively controls the information storage unit 31 and the information extraction unit 32 described above.
  • FIG. 5 is a block diagram illustrating a configuration example of the image display device 4.
  • the image display device (viewer) 4 includes a display unit 41, a sample recognition unit 42, a sample information acquisition unit 43, an access information storage unit 44, a CPU 45, an image data acquisition unit 46, an information input unit 47, and The input / output interface unit 48 is configured.
  • the image display device (viewer) 4 may be any device that can be connected to the information processing device 2 and the server 3 via the network 5, and may be a portable terminal such as a tablet terminal.
  • sample recognition unit 42 reads identification information of a pathological specimen to be browsed. For example, when the identification information is recorded in a barcode or an RF tag, the sample recognition unit 42 is a reader.
  • the identification information of the reading target may be described or attached to a pathological specimen (slide glass) actually observed with a microscope, as well as a pathological specimen such as an examination table or medical chart. is there.
  • sample information acquisition unit 43 transmits the identification information acquired by the sample recognition unit 42 to the server 3, and information (access information) for accessing data of a microscope observation image obtained by imaging the pathological specimen to be browsed from the server 3. To get.
  • the access information storage unit 44 is provided as necessary, and stores the access information transmitted from the server 3. If the access information to the image data to be browsed is stored in this way, for example, when the viewer transmits an image data acquisition request to the server, position information and enlargement / reduction information are added to the access information. It becomes possible to generate request information easily. Thereby, the freedom degree of operation at the time of browsing a microscope observation image, such as expansion / contraction, increases.
  • the image data acquisition unit 46 accesses the server 3 based on the access information acquired by the sample information acquisition unit 43 or stored in the access information storage unit 44, and acquires image data of the microscopic observation image to be browsed.
  • the image data acquisition unit 46 may acquire the corresponding image data by designating an area, a magnification, and the like based on information about the display image input to the information input unit 47.
  • the image display device (viewer) 4 may be provided with a display information input unit 47 into which information related to a microscope observation image displayed by the user is input.
  • the information input to the information input unit 47 includes, for example, a display area and display magnification of a microscope observation image, or selection information when a plurality of access information exists in a pathological specimen to be browsed.
  • Information input by the user is transmitted to the server 3 via the image data acquisition unit 46 or the network 5.
  • Display unit 41 The display unit 41 displays the related information in the microscope observation image, and any display unit 41 may be used.
  • the CPU 45 comprehensively controls each unit provided in the image display device 4 and controls, for example, the sample recognition unit 42, the sample information acquisition unit 43, the access information storage unit 44, the image data acquisition unit 46, and the like described above. Program to control automatically.
  • the network 5 is a communication line network that connects the information processing device 2, the server 3, and the image display device 4 to each other so that bidirectional communication is possible.
  • the network 5 includes, for example, the public network such as the Internet, a telephone line network, a satellite communication network, a broadcast communication path, a WAN (Wide Area Network), a LAN (Local Area Network), an IP-VPN (Internet Protocol-Virtual).
  • a private network such as Private Network, Ethernet (registered trademark), or a wireless LAN is used, regardless of whether it is wired or wireless.
  • the network 5 may be a communication line network provided exclusively for the microscope system of the present embodiment.
  • FIG. 6 is a flowchart showing the overall operation of the microscope system of the present embodiment
  • FIGS. 7, 10, and 12 to 14 are flowcharts showing the respective steps.
  • FIG. 8A is a figure which shows an example of a pathological sample
  • FIG. 8B is a figure which shows the label.
  • FIG. 9 is a diagram showing the relationship between the sample number and the slide
  • FIG. 11 is a diagram showing an example of access information.
  • a microscope observation image of a pathological specimen is captured by the digital microscope 1.
  • the identification information recognition unit 22 of the information processing apparatus 2 acquires the identification information from the pathological specimen to be imaged.
  • the barcode 103 attached to the label 102 of the slide 100 the barcode connected to the imaging function of the digital microscope 1 or the information processing device 2. It can be read by a reader or the like.
  • the identification information read here is the number assigned to each slide, for example, in the case where a plurality of slides having different slice positions for the same specimen are prepared in addition to the sample number. In some cases.
  • the specimen 101 of the pathological specimen is imaged, and the obtained image is processed by the image processing unit 23 of the information processing apparatus 2 to obtain a microscope observation image.
  • the identification information acquired by the identification information recognition unit 22 and the microscope observation image synthesized by the image processing unit 23 are updated to the information storage unit 31 of the server 3.
  • the uploaded image data and the identification information are associated with each other, and information (access information) for accessing the image data is generated.
  • the format of this access information is not particularly limited, and there is, for example, an access destination URL as shown in FIG.
  • the image data of the microscope observation image is stored in the image data storage unit 312 and access information to the image data is stored in the specimen information storage unit 313 together with the identification information.
  • the sample recognition unit 42 of the image display device 4 attaches to a slide glass, an inspection table, or a medical chart.
  • the identification information of the pathological specimen to be viewed is read.
  • the sample information acquisition unit 43 transmits the identification information acquired by the sample recognition unit 42 to the server 3, and information for accessing the data of the microscope observation image obtained by imaging the pathological specimen to be browsed from the server 3 ( Access information).
  • the server 3 that has received the request for the identification information extracts the access information of the requested microscope observation image from the image display device 4 in the information extraction unit 32 and transmits the access information to the image display device 4. .
  • the access information provided from the server 3 is stored in the access information storage unit 44 as necessary.
  • FIG. Check when displaying a microscope observation image on the display unit 41 of the image display device (viewer) 4 based on the access information, first, as shown in FIG. Check.
  • information is input (Y)
  • the input content is a change in the viewpoint or magnification of the image displayed on the display unit 41 of the image display device (viewer) 4
  • the determination is made based on whether or not the data (tile image etc.) necessary for display is available. Is done.
  • the image data acquisition unit 46 accesses the server 3 based on the access information acquired by the sample information acquisition unit 43 or the access information stored in the access information storage unit 44, and the image data of the microscopic observation image to be browsed To get. Then, the image data provided from the server 3 is displayed on the display unit 41 of the image display device (viewer) 4.
  • identification information read from a slide, an examination table, or a medical chart is transmitted to a server, and access information of image data associated with the identification information is acquired. Images can be easily and reliably selected and displayed. Further, since it is not necessary for the user to directly input identification information to search or to select a plurality of images while browsing, the image to be selected is not mistaken.
  • the type of identification information displayed (disclosed) can be changed or restricted for each user.
  • the server 3 associates image data with its identification information and generates access information, but the present disclosure is not limited to this.
  • the information processing apparatus 2 may have these functions without providing the server 3.
  • an information storage unit and an information extraction unit may be provided in the information processing device 2, and the image display device 4 requests access information from the information processing device 2.
  • FIG. 15 is a block diagram illustrating a configuration example of the image display apparatus of the microscope system according to the present embodiment
  • FIG. 16 is a flowchart illustrating the operation of the server 3 when providing image data to the image display apparatus 6.
  • the image display device acquires access information to the microscope observation image data.
  • the present disclosure is not limited to this, and the access information is not acquired.
  • Microscopic observation image data can also be acquired.
  • the image display device 6 may be provided with a sample recognition unit 42 and an image data acquisition unit 46.
  • the sample recognition unit 42 of the image display device 6 identifies the pathological specimen to be browsed attached to the slide glass, examination table, medical chart, or the like. Read information.
  • the image data acquisition unit 46 transmits the identification information acquired by the sample recognition unit 42 to the server 3, and acquires data of a microscope observation image obtained by imaging the pathological specimen to be browsed from the server 3.
  • the pathological specimen identification information is transmitted to the server and the image data of the pathological specimen is acquired, so that the target microscope observation image can be easily and reliably selected and displayed. be able to.
  • the configuration and effects other than those described above in the microscope system of the present embodiment are the same as those in the first embodiment described above.
  • the present disclosure can take the following configurations.
  • a sample recognition unit for reading identification information of a pathological specimen to be browsed;
  • a sample information acquisition unit for transmitting the identification information to an information processing device and acquiring information on the pathological specimen from the information processing device;
  • An image display apparatus (2) The image display apparatus according to (1), wherein the information related to the pathological specimen is microscope observation image data obtained by imaging the pathological specimen or access information to the microscope observation image data.
  • the image display device according to (2) further including an access information storage unit that stores the access information.
  • the image display device further including an information input unit through which a user inputs information related to the microscope observation image data.
  • the sample recognition unit is a barcode or RF tag reader.
  • the identification information is attached to a slide glass in which the pathological specimen is enclosed.
  • the image display device further including a display unit capable of browsing a microscope observation image obtained by imaging the pathological specimen.
  • An image display method comprising: (10) In an image display device comprising at least a sample recognition unit and a sample information acquisition unit, A sample recognition function that reads the identification information of the pathological specimen to be browsed; A sample information acquisition function for transmitting the identification information to an information processing device and acquiring information on the pathological specimen from the information processing device;
  • a program that executes (11) A sample recognition unit that reads identification information of a pathological specimen to be browsed;
  • An image display device comprising at least An information storage unit storing information on the pathological specimen;
  • An information extraction unit that extracts information to be provided to the image display device from the information storage unit
  • the information storage unit of the information processing apparatus stores a microscope observation image of a pathological specimen imaged by a microscope apparatus, and identification information of the pathological specimen associated with the microscope observation image,
  • the information extracting unit extracts data of a microscope observation image associated with the identification information or access information to the microscope observation image data based on the identification information transmitted from the image display device (11).
  • Microscope system

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Abstract

La présente invention concerne un dispositif d'affichage d'image, un procédé d'affichage d'image, un programme et un système de microscope qui permettent de sélectionner et d'afficher facilement et sans erreur une image spécifique d'une observation au microscope. Ledit dispositif d'affichage d'image, qui affiche des images capturées par un microscope, est muni : d'une unité de reconnaissance d'échantillons servant à lire des informations d'identification d'un spécimen de pathologie à observer ; et d'une unité d'acquisition d'informations d'échantillon conçue pour envoyer les informations d'identification lues par ladite unité de reconnaissance d'échantillon à un dispositif de traitement d'informations, et acquérir à partir dudit dispositif de traitement d'informations des informations se rapportant au spécimen de pathologie à observer.
PCT/JP2013/071784 2012-09-24 2013-08-12 Dispositif d'affichage d'image, procédé, programme et système de microscope WO2014045759A1 (fr)

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JP2018180281A (ja) * 2017-04-13 2018-11-15 浜松ホトニクス株式会社 画像取得システム及び画像取得方法
US11022621B2 (en) 2017-04-13 2021-06-01 Hamamatsu Photonics K.K. Image acquisition system and image acquisition method
CN112329494A (zh) * 2020-10-16 2021-02-05 湖北三赢兴光电科技股份有限公司 基于电子显微镜的二维码识别方法及设备

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