WO2013156895A3 - Método rápido y confiable de identificación de bacterias lácticas en vino - Google Patents

Método rápido y confiable de identificación de bacterias lácticas en vino Download PDF

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Publication number
WO2013156895A3
WO2013156895A3 PCT/IB2013/052802 IB2013052802W WO2013156895A3 WO 2013156895 A3 WO2013156895 A3 WO 2013156895A3 IB 2013052802 W IB2013052802 W IB 2013052802W WO 2013156895 A3 WO2013156895 A3 WO 2013156895A3
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WO
WIPO (PCT)
Prior art keywords
sample
wine
rapid
dna
reliable method
Prior art date
Application number
PCT/IB2013/052802
Other languages
English (en)
French (fr)
Other versions
WO2013156895A2 (es
Inventor
Jaime Moisés ROMERO ORMAZÁBAL
Carolina Alejandra ILABACA DÍAZ
Original Assignee
Universidad De Chile
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad De Chile filed Critical Universidad De Chile
Priority to US14/395,276 priority Critical patent/US20150152483A1/en
Publication of WO2013156895A2 publication Critical patent/WO2013156895A2/es
Publication of WO2013156895A3 publication Critical patent/WO2013156895A3/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La invención describe un método para identificar las bacterias Oenococcus, Leuconostoc, Pediococcus y Lactobacillus en una muestra de fermentación, que comprende los pasos de a) obtención de una muestra desde una fermentación; b) eliminación de sustancias colorantes, fenoles y otras sustancias inhibidoras del PCR de la muestra; c) lisis de las bacterias presentes en la muestra; d) extracción del DNA de las muestras; e) amplificación por PCR del DNA extraído usando partidores universales diseñados para una zona objetivo del gen 16S r RNA, f) digestión de los amplificados usando una solución de restricción con enzimas elegidas por análisis teórico; g) incubación; h) electroforesis de los fragmentos obtenidos. La invención también provee un kit para aplicar el método descrito.
PCT/IB2013/052802 2012-04-19 2013-04-08 Método rápido y confiable de identificación de bacterias lácticas en vino WO2013156895A2 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/395,276 US20150152483A1 (en) 2012-04-19 2013-04-08 Fast and reliable wine lactic bacteria identification method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CL2012-00993 2012-04-19
CL2012000993A CL2012000993A1 (es) 2012-04-19 2012-04-19 Metodo para identificar las bacterias oenococcus, leuconostoc, pediococcus y lactobacillus en una muestra de fermentacion, y kit para el desarrollo de dicho proceso de identificacion.

Publications (2)

Publication Number Publication Date
WO2013156895A2 WO2013156895A2 (es) 2013-10-24
WO2013156895A3 true WO2013156895A3 (es) 2014-01-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2013/052802 WO2013156895A2 (es) 2012-04-19 2013-04-08 Método rápido y confiable de identificación de bacterias lácticas en vino

Country Status (2)

Country Link
CL (1) CL2012000993A1 (es)
WO (1) WO2013156895A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111662838B (zh) * 2020-01-21 2022-01-28 甘肃农业大学 一种产酯酶苹果酸-乳酸发酵乳酸菌菌株及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06141899A (ja) * 1992-11-04 1994-05-24 Sapporo Breweries Ltd 乳酸菌の高感度検出法
WO2002012560A1 (en) * 2000-08-04 2002-02-14 E. & J. Gallo Winery Method of amplifying nucleic acids of microorganisms present in fruit juice

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06141899A (ja) * 1992-11-04 1994-05-24 Sapporo Breweries Ltd 乳酸菌の高感度検出法
WO2002012560A1 (en) * 2000-08-04 2002-02-14 E. & J. Gallo Winery Method of amplifying nucleic acids of microorganisms present in fruit juice

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHENOLL, E. ET AL.: "Identification of Carnobacterium, Lactobacillus, Leuconostoc and Pediococcus by rDNA-based techniques", SYSTEMATIC AND APPLIED MICROBIOLOGY, vol. 26, no. 4, 2003, pages 546 - 556 *
DU PLESSIS, H.W. ET AL.: "Identification of lactic acid bacteria isolated from South African brandy base wines", INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, vol. 91, no. 1, 2004, pages 19 - 29 *
ENDO, A. ET AL.: "Monitoring the lactic acid bacterial diversity during shochu fermentation by PCR-denaturing gradient gel electrophoresis", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 99, no. 3, 2005, pages 216 - 221 *
LOPEZ, I. ET AL.: "Design and Evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 69, no. 11, 2003, pages 6801 - 6807 *
NAVARRETE, P. ET AL.: "Molecular analysis of intestinal microbiota of rainbow trout (Oncorhynchus mykiss)", FEMS MICROBIOLOGY ECOLOGY, vol. 71, no. 1, 2010, pages 148 - 156 *
RODAS, A.M. ET AL.: "16S-ARDRA, a tool for identification of lactic acid bacteria isolated from grape must and wine", SYSTEMATIC AND APPLIED MICROBIOLOGY, vol. 26, no. 3, 2003, pages 412 - 422 *
SOLIERI, L. ET AL.: "Characterization and technological properties of Oenococcus oeni strains from wine spontaneous malolactic fermentations: a framework for selection of new starter cultures", JOURNAL OF APPLIED MICROBIOLOGY, vol. 108, no. 1, 2010, pages 285 - 298 *

Also Published As

Publication number Publication date
WO2013156895A2 (es) 2013-10-24
CL2012000993A1 (es) 2012-07-27

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