Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
  • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
  • G01N33/54366—Apparatus specially adapted for solid-phase testing
  • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
  • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
  • G01N21/55—Specular reflectivity
  • G01N21/552—Attenuated total reflection
  • G01N21/553—Attenuated total reflection and using surface plasmons
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
  • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
  • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
  • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
  • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
  • G01N33/54346—Nanoparticles
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
  • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
  • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
  • G01N33/553—Metal or metal coated
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N2201/00—Features of devices classified in G01N21/00
  • G01N2201/06—Illumination; Optics
  • G01N2201/061—Sources
  • G01N2201/06113—Coherent sources; lasers
  • G01N2201/0612—Laser diodes
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N2201/00—Features of devices classified in G01N21/00
  • G01N2201/06—Illumination; Optics
  • G01N2201/061—Sources
  • G01N2201/06146—Multisources for homogeneisation, as well sequential as simultaneous operation
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N2201/00—Features of devices classified in G01N21/00
  • G01N2201/06—Illumination; Optics
  • G01N2201/062—LED's
  • G01N2201/0621—Supply
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N2201/00—Features of devices classified in G01N21/00
  • G01N2201/06—Illumination; Optics
  • G01N2201/063—Illuminating optical parts
  • G01N2201/0635—Structured illumination, e.g. with grating
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N2201/00—Features of devices classified in G01N21/00
  • G01N2201/12—Circuits of general importance; Signal processing
  • G01N2201/127—Calibration; base line adjustment; drift compensation
  • G01N2201/12723—Self check capacity; automatic, periodic step of checking

Definitions

• the first signal was recorded before a sample is brought into contact with the layer and the second signal was recorded after the sample is brought into contact with the layer.
• the method allows a user to detect small changes of refractive index at the conductive layer due to a liquid sample.
• probes are immobilised on the layer and the sample includes targets, such that the change in refractive index is due to targets binding to probes on the layer (and remaining there after a separate wash). This is particularly advantageous as the method can be used to detect small amounts of target present in a blood sample, blood plasma sample or other liquids.
• the analytical device (1 ) may be used to probe or quantify events or elements in a liquid sample placed or flowing into at least one detection zone (30), and measurements maybe performed as a function of time. Preferably the device (1 ) determines a sensorgram, thereby providing the temporal variation of at least one parameter which affects Surface Plasmon Resonance.
• the optical module (60) contains two diode lasers (62) and (63) in some embodiments.
• the diode lasers emit at 785nm and 808nm, and are aligned perpendicularly.
• a beam splitter consisting of a glass plate of 0.1 mm of thickness and 15nm of reflective metal layer, with the thickness adjusted in order to have -50% of light transmission at ⁇ 800nm of wavelength is provided.
• An acrylic cylinder (65) preferably focuses the light beam (100) into the grating surface (40) of the detection zone (30).
• the first order reflective diffraction passes into a polarizer (90) and is incident into the optical detector, preferably a CMOS camera (80).
• the motor (50) may be a standard BLDC motor, which can be controlled with rotational speeds between 5Hz and 150Hz. Both the motor (50) and the optical module (60) can be attached to a base (70) and the whole system is preferably temperature controlled by external components.
• the SPR dips need to be within a predefined angular position [e.g. 54.5° +/- 0.1 ° for the first SPR dip and 55.5° +/- 0.1 ° for the second SPR dip] for the initial SPR measurements prior to passing a sample and the mutual spacing between the two SPR dips need to be within another predefined angular range [e.g. 1 .0° +/- 0.1 °]. In this case measurements are rejected if they do not comply with the acceptance criteria.
• the intensity level of the initial peak Ai between the two SPR deeps need to be a pre-defined fraction of the maximum measured light intensity, for example 70% +/- 1 % of the maximum measured light intensity. Measurements are rejected if they do not comply with all the applicable acceptance criteria, in some embodiments.
• Additional relevant acceptance criteria may include, but are not limited to, the relative difference of light intensity of the two SPR dips; the relative angular spacing between the initial peak Ai and the two SPR dips m1 i and m2i; the relative difference of shift of each of the two SPR dips, etc.
• the detection surface has a grating surface of a sinusoidal shape, trapezoidal shape or triangular shape, instead of a sine-trapezoidal shape.
• a grating surface of a sinusoidal shape, trapezoidal shape or triangular shape, instead of a sine-trapezoidal shape.
• Each specific shape of the grating surface will result in a different and characteristic shape of each SPR dip, in particular expected SPR dip width and symmetry. Accordingly, where quality or feedback control as described above is employed, the parameters of acceptance criteria are adjusted for each particular grating implementation.
• detection systems have a detection surface with a flat conductive surface and use a prism configuration to achieve the momentum coupling required for SPR to occur.
• the SPR dips have a known pre-defined shape.
• microfluidic is referred to herein to mean devices having a fluidic element such as a reservoir or a channel with at least one dimension below I mm.
• a Surface Plasmon Resonance (SPR) sensing method comprising the steps of: providing a SPR sensor comprising a SPR supporting sensor surface;

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• Health & Medical Sciences (AREA)
• Immunology (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• Biochemistry (AREA)
• General Health & Medical Sciences (AREA)
• General Physics & Mathematics (AREA)
• Physics & Mathematics (AREA)
• Pathology (AREA)
• Analytical Chemistry (AREA)
• Hematology (AREA)
• Biomedical Technology (AREA)
• Molecular Biology (AREA)
• Urology & Nephrology (AREA)
• Biotechnology (AREA)
• Cell Biology (AREA)
• Microbiology (AREA)
• Food Science & Technology (AREA)
• Medicinal Chemistry (AREA)
• Nanotechnology (AREA)
• Inorganic Chemistry (AREA)
• Investigating Or Analysing Materials By Optical Means (AREA)

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• 2013
  • 2013-03-05 JP JP2014560342A patent/JP6100803B2/ja not_active Expired - Fee Related
  • 2013-03-05 US US14/382,334 patent/US9632022B2/en not_active Expired - Fee Related
  • 2013-03-05 EP EP13707633.7A patent/EP2823287B1/en not_active Not-in-force
  • 2013-03-05 WO PCT/EP2013/054446 patent/WO2013131928A1/en not_active Ceased
• 2017
  • 2017-04-12 US US15/486,039 patent/US10228368B2/en active Active

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