WO2013109055A3 - 개 유래의 자연살해세포 및 이의 대량 증식방법 - Google Patents

개 유래의 자연살해세포 및 이의 대량 증식방법 Download PDF

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Publication number
WO2013109055A3
WO2013109055A3 PCT/KR2013/000352 KR2013000352W WO2013109055A3 WO 2013109055 A3 WO2013109055 A3 WO 2013109055A3 KR 2013000352 W KR2013000352 W KR 2013000352W WO 2013109055 A3 WO2013109055 A3 WO 2013109055A3
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WIPO (PCT)
Prior art keywords
natural killer
killer cells
canine
derived natural
proliferation method
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PCT/KR2013/000352
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English (en)
French (fr)
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WO2013109055A2 (ko
Inventor
김상기
신동준
박지윤
조덕
Original Assignee
공주대학교 산학협력단
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Application filed by 공주대학교 산학협력단 filed Critical 공주대학교 산학협력단
Publication of WO2013109055A2 publication Critical patent/WO2013109055A2/ko
Publication of WO2013109055A3 publication Critical patent/WO2013109055A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4613—Natural-killer cells [NK or NK-T]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Oncology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 개 유래의 자연살해세포 및 이의 대량 증식방법에 관한 것으로，본 발명의 개 유래의 자연살해세포는 개의 암 또는 바이러스 질환， 질병 또는 증상의 예방 또는 치료제로서 사용가능하며, 본 발명에 따른 자연살해세포 대량 증식방법은 개 유래의 자연살해세포의 살해능을 현저히 증가시키면서 최대 증식이 가능한 획기적인 방법이므로， 적은 양의 채혈로 많은 수의 자연살해세포를 수득할 수 있게 됨으로써 세포치료제로의 상용화에 크게 기여할 것이다.
PCT/KR2013/000352 2012-01-17 2013-01-17 개 유래의 자연살해세포 및 이의 대량 증식방법 WO2013109055A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2012-0005272 2012-01-17
KR1020120005272A KR101384203B1 (ko) 2012-01-17 2012-01-17 개 유래의 자연살해세포의 대량 증식방법

Publications (2)

Publication Number Publication Date
WO2013109055A2 WO2013109055A2 (ko) 2013-07-25
WO2013109055A3 true WO2013109055A3 (ko) 2013-08-29

Family

ID=48799774

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2013/000352 WO2013109055A2 (ko) 2012-01-17 2013-01-17 개 유래의 자연살해세포 및 이의 대량 증식방법

Country Status (2)

Country Link
KR (1) KR101384203B1 (ko)
WO (1) WO2013109055A2 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101499345B1 (ko) * 2013-09-17 2015-03-04 공주대학교 산학협력단 개 자연살해세포 체외 증폭배지 및 이를 이용한 개 자연살해세포 체외 배양방법
KR102069704B1 (ko) * 2018-05-16 2020-01-23 고려대학교 산학협력단 Hdac 억제제를 이용한 사람 유래 자연살해세포의 확장 배양법
JP2022529280A (ja) * 2019-04-17 2022-06-20 チャ バイオテック カンパニー リミテッド 抗癌活性が増大されたナチュラルキラー細胞、及びその免疫治療用途
KR20220135994A (ko) * 2021-03-31 2022-10-07 주식회사 씨티셀즈 자연 살해 세포 분리 장치 및 그의 자연 살해 세포 분리 방법
KR20230039789A (ko) * 2021-09-10 2023-03-21 한국생명공학연구원 자연살해세포의 제조방법 및 이의 감염성 질환 예방 또는 치료 용도
CN115125204B (zh) * 2022-07-19 2023-08-22 百欧派(天津)生物技术有限公司 具有犬自然杀伤细胞体外激活功能的组合物及体外培养的方法
CN115094037B (zh) * 2022-07-19 2023-11-10 百欧派(天津)生物技术有限公司 配合自体血浆使用的具有犬自然杀伤细胞体外激活功能的组合物及其用途

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020068044A1 (en) * 1997-04-30 2002-06-06 Hans Klingemann Natural killer cell lines and methods of use

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040197903A1 (en) 2003-01-31 2004-10-07 Northwest Biotherapeutics, Inc. Method for induction of proliferation of natural killer cells by dendritic cells cultured with GM-CSF and IL-15

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020068044A1 (en) * 1997-04-30 2002-06-06 Hans Klingemann Natural killer cell lines and methods of use

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BONKOBARA, M. ET AL.: "Characterization of cDNA and the genomic sequence encoding canine neural cell adhesion molecule CD56 N CAM", VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, vol. 107, no. 1, 15 August 2005 (2005-08-15), pages 171 - 176, XP004952309 *
CHO, DEOK ET AL.: "Selective Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells by K562 Cell Line and IL-2", KOREAN JOURNAL OF HEMATOLOGY, vol. 41, no. 1, 31 March 2006 (2006-03-31), pages 8 - 15 *
HUANG, YI CHUN ET AL.: "CD5 low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells", JOURNAL OF LEUKOCYTE BIOLOGY, vol. 84, no. 6, 15 August 2008 (2008-08-15), pages 1501 - 1510, XP055082424 *
LIN, YI-CHUN ET AL.: "Canine CD8 T cells showing NK cytotoxic activity express mRNAs for NK cell associated surface molecules", VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, vol. 133, no. 2, 5 August 2009 (2009-08-05), pages 144 - 153, XP026816594 *

Also Published As

Publication number Publication date
KR20130084465A (ko) 2013-07-25
WO2013109055A2 (ko) 2013-07-25
KR101384203B1 (ko) 2014-04-10

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