WO2013089478A3 - 신규 ｏ-포스포세린 설프하이드릴라아제를 이용하여 시스테인 또는 이의 유도체를 생산하는 방법 - Google Patents

신규 ｏ-포스포세린 설프하이드릴라아제를 이용하여 시스테인 또는 이의 유도체를 생산하는 방법 Download PDF

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Publication number
WO2013089478A3
WO2013089478A3 PCT/KR2012/010900 KR2012010900W WO2013089478A3 WO 2013089478 A3 WO2013089478 A3 WO 2013089478A3 KR 2012010900 W KR2012010900 W KR 2012010900W WO 2013089478 A3 WO2013089478 A3 WO 2013089478A3
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WO
WIPO (PCT)
Prior art keywords
novel
derivative
cysteine
preparing
phosphoserine sulfhydrylase
Prior art date
Application number
PCT/KR2012/010900
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English (en)
French (fr)
Other versions
WO2013089478A2 (ko
Inventor
송병철
장진숙
조재현
김혜원
Original Assignee
씨제이제일제당 (주)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES12858268.1T priority Critical patent/ES2634683T3/es
Priority to RU2014125851/10A priority patent/RU2579689C1/ru
Priority to BR112014014762-0A priority patent/BR112014014762B1/pt
Priority to US14/365,571 priority patent/US9243268B2/en
Priority to CA2859125A priority patent/CA2859125C/en
Priority to CN201280067058.7A priority patent/CN104039963B/zh
Application filed by 씨제이제일제당 (주) filed Critical 씨제이제일제당 (주)
Priority to BR122019023670-4A priority patent/BR122019023670B1/pt
Priority to JP2014547104A priority patent/JP5860550B2/ja
Priority to EP12858268.1A priority patent/EP2792748B1/en
Publication of WO2013089478A2 publication Critical patent/WO2013089478A2/ko
Publication of WO2013089478A3 publication Critical patent/WO2013089478A3/ko
Priority to PH12014501353A priority patent/PH12014501353A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01065—O-Phosphoserine sulfhydrylase (2.5.1.65)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

본 발명은 신규 O-포스포세린 설프하이드릴라아제(O-phosphoserine sulfhydrylase)를 이용하여 시스테인 또는 이의 유도체를 생산하는 방법에 관한 것이다. 본 발명에 따라 신규 O-포스포세린 설프하이드릴라아제(O-phosphoserine sulfhydrylase, OPSS)를 이용하여 O-포스포세린을 기질로 하여 시스테인을 생산하는 방법을 제공함으로써, 이를 이용하여 간편한 방법으로 고수율로 친환경적인 시스테인을 용이하게 생산할 수 있다는 이점이 있다.
PCT/KR2012/010900 2011-12-15 2012-12-14 신규 ｏ-포스포세린 설프하이드릴라아제를 이용하여 시스테인 또는 이의 유도체를 생산하는 방법 WO2013089478A2 (ko)

Priority Applications (10)

Application Number Priority Date Filing Date Title
RU2014125851/10A RU2579689C1 (ru) 2011-12-15 2012-12-14 Способ получения цистеина или его производного с использованием новой о-фосфосеринсульфгидрилазы
BR112014014762-0A BR112014014762B1 (pt) 2011-12-15 2012-12-14 Método para a preparação de cisteína ou de um derivado desta usando uma nova o- fosfoserina sulfidrilase
US14/365,571 US9243268B2 (en) 2011-12-15 2012-12-14 Method for preparing cysteine or a derivative thereof using a novel O-phosphoserine sulfhydrylase
CA2859125A CA2859125C (en) 2011-12-15 2012-12-14 Method for preparing cysteine or a derivative thereof using a novel o-phosphoserine sulfhydrylase
CN201280067058.7A CN104039963B (zh) 2011-12-15 2012-12-14 利用新o-磷酸丝氨酸巯解酶生产半胱氨酸或其衍生物的方法
ES12858268.1T ES2634683T3 (es) 2011-12-15 2012-12-14 Método de preparación de cisteína o de un derivado de la misma utilizando una nueva O-fosfoserina sulfhidrilasa
BR122019023670-4A BR122019023670B1 (pt) 2011-12-15 2012-12-14 Método para a preparação de cisteína ou de um derivado desta usando uma nova ofosfoserina sulfidrilase
JP2014547104A JP5860550B2 (ja) 2011-12-15 2012-12-14 新規なｏ−ホスホセリンスルフヒドリラーゼを用いたシステイン又はその誘導体の製造方法
EP12858268.1A EP2792748B1 (en) 2011-12-15 2012-12-14 Method for preparing cysteine or a derivative thereof using a novel o-phosphoserine sulfhydrylase
PH12014501353A PH12014501353A1 (en) 2011-12-15 2014-06-13 Method for preparing cysteine or a derivative thereof using a novel o-phosphoserine sulfhydrylase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0135665 2011-12-15
KR1020110135665A KR101404376B1 (ko) 2011-12-15 2011-12-15 신규 ｏ-포스포세린 설프하이드릴라아제를 이용하여 시스테인 또는 이의 유도체를 생산하는 방법

Publications (2)

Publication Number Publication Date
WO2013089478A2 WO2013089478A2 (ko) 2013-06-20
WO2013089478A3 true WO2013089478A3 (ko) 2013-08-22

Family

ID=48613316

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/010900 WO2013089478A2 (ko) 2011-12-15 2012-12-14 신규 ｏ-포스포세린 설프하이드릴라아제를 이용하여 시스테인 또는 이의 유도체를 생산하는 방법

Country Status (12)

Country Link
US (1) US9243268B2 (ko)
EP (1) EP2792748B1 (ko)
JP (1) JP5860550B2 (ko)
KR (1) KR101404376B1 (ko)
CN (1) CN104039963B (ko)
BR (2) BR112014014762B1 (ko)
CA (2) CA2914131C (ko)
ES (1) ES2634683T3 (ko)
MY (2) MY176977A (ko)
PH (1) PH12014501353A1 (ko)
RU (1) RU2579689C1 (ko)
WO (1) WO2013089478A2 (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101677328B1 (ko) 2014-08-12 2016-11-18 씨제이제일제당 (주) O-포스포세린 생산 미생물 및 이를 이용한 o-포스포세린 또는 l-시스테인 생산 방법
KR101694632B1 (ko) * 2015-09-11 2017-01-10 씨제이제일제당 (주) 신규 ｏ-포스포세린 배출 단백질 변이체 및 이를 이용한 ｏ-포스포세린, 시스테인 및 이의 유도체의 생산방법
KR101825310B1 (ko) * 2016-12-29 2018-03-15 씨제이제일제당 (주) O-포스포세린을 생산하는 에스케리키아 속 미생물 및 이를 이용한 o-포스포세린 또는 l-시스테인을 생산하는 방법
KR102025867B1 (ko) 2017-07-13 2019-09-27 씨제이제일제당 주식회사 인산을 발효액 또는 발효 폐액으로부터 회수 및 재사용하는 방법
KR20190092951A (ko) 2018-01-31 2019-08-08 씨제이제일제당 (주) 연속식 크로마토그래피 공정을 이용한 천연 l-시스테인 결정의 제조 방법
KR20190092950A (ko) 2018-01-31 2019-08-08 씨제이제일제당 (주) 연속식 크로마토그래피 공정을 이용한 천연 l-시스테인 염산염 수화물 결정의 제조 방법

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US20070026505A1 (en) * 2005-06-17 2007-02-01 Madden Kevin T Amino acid and metabolite biosynthesis
US20090053778A1 (en) * 2004-07-20 2009-02-26 France Telecom Microorganisms for producing sulfur-containing compounds

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CA1299128C (en) 1986-11-19 1992-04-21 Tooru Miyahara Method of producing l-cystine
US6579705B2 (en) 2001-04-04 2003-06-17 Consortium Fur Elektrochemische Industrie Gmbh Process for preparing non-proteinogenic L-amino acids
RU2279477C2 (ru) * 2003-12-05 2006-07-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Мутантная серинацетилтрансфераза, фрагмент днк, кодирующий мутантную серинацетилтрансферазу (варианты), бактерия, принадлежащая к роду escherichia, - продуцент l-цистеина, и способ продукции l-цистеина
JP2006304673A (ja) * 2005-04-28 2006-11-09 National Institute Of Advanced Industrial & Technology システイン合成酵素のホスホセリンに対する基質特異性を高める方法
KR101381048B1 (ko) * 2010-10-20 2014-04-14 씨제이제일제당 (주) Ｏ-포스포세린 생산 균주 및 이로부터 생산된 ｏ-포스포세린으로부터 ｌ-시스테인 또는 이의 유도체의 생산방법

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US20090053778A1 (en) * 2004-07-20 2009-02-26 France Telecom Microorganisms for producing sulfur-containing compounds
US20070026505A1 (en) * 2005-06-17 2007-02-01 Madden Kevin T Amino acid and metabolite biosynthesis

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AGREN, DANIEL E ET AL.: "CYSTEINE SYNTHASE (CYSM) OF MYCOBACTERIUM TUBERCULOSIS IS AN O-PHOSPHOSERINE SULFHYDRYLASE: EVIDENCE FOR AN ALTERNATIVE CYSTEINE BIOSYNTHESIS PATHWAY IN MYCOBACTERIA", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 283, no. 46, 2008, pages 31567 - 31574, XP055014144 *
AGREN, DANIEL ET AL.: "THE C-TERMINAL OF CYSM FROM MYCOBACTERIUM TUBERCULOSIS PROTECTS THE AMINOACRYLATE INTERMEDIATE AND IS INVOLVED IN SULFUR DONOR SELECTIVITY", FEBS LETTERS., vol. 583, no. 2, 2009, pages 330 - 336, XP025926474 *
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Also Published As

Publication number Publication date
MY176977A (en) 2020-08-28
US20150004657A1 (en) 2015-01-01
EP2792748B1 (en) 2017-04-26
MY164371A (en) 2017-12-15
US9243268B2 (en) 2016-01-26
CA2859125C (en) 2018-05-01
CN104039963B (zh) 2016-08-31
JP5860550B2 (ja) 2016-02-16
CA2914131A1 (en) 2013-06-20
CA2859125A1 (en) 2013-06-20
CN104039963A (zh) 2014-09-10
PH12014501353B1 (en) 2014-09-22
EP2792748A2 (en) 2014-10-22
BR122019023670B1 (pt) 2021-06-29
BR112014014762A2 (ko) 2018-05-22
WO2013089478A2 (ko) 2013-06-20
KR101404376B1 (ko) 2014-06-11
JP2015500039A (ja) 2015-01-05
KR20130068135A (ko) 2013-06-25
RU2579689C1 (ru) 2016-04-10
PH12014501353A1 (en) 2014-09-22
BR112014014762A8 (pt) 2019-11-19
CA2914131C (en) 2018-05-01
EP2792748A4 (en) 2015-08-05
ES2634683T3 (es) 2017-09-28
BR112014014762B1 (pt) 2021-06-29

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