WO2013036049A3 - 공초점 형광 현미경 - Google Patents

공초점 형광 현미경 Download PDF

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Publication number
WO2013036049A3
WO2013036049A3 PCT/KR2012/007190 KR2012007190W WO2013036049A3 WO 2013036049 A3 WO2013036049 A3 WO 2013036049A3 KR 2012007190 W KR2012007190 W KR 2012007190W WO 2013036049 A3 WO2013036049 A3 WO 2013036049A3
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WO
WIPO (PCT)
Prior art keywords
light source
fluorescence
unit
radiates
outputted
Prior art date
Application number
PCT/KR2012/007190
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English (en)
French (fr)
Other versions
WO2013036049A2 (ko
Inventor
홍성철
이진우
Original Assignee
서울대학교산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 서울대학교산학협력단 filed Critical 서울대학교산학협력단
Priority to US14/342,966 priority Critical patent/US9563046B2/en
Publication of WO2013036049A2 publication Critical patent/WO2013036049A2/ko
Publication of WO2013036049A3 publication Critical patent/WO2013036049A3/ko

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/0048—Scanning details, e.g. scanning stages scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

본 발명은 세포나 조직 내부에서 단일 형광 분자 신호를 실시간으로 (수십 Hz 이상) 관측하고자 하는데 그 목적이 있다. 위의 과제를 해결하고자 본 발명은 크게 하나 이상의 단파장 레이저로 이루어진 광원부, 평행광인 광원을 적절한 크기의 선형의 빛으로 만드는 렌즈부, 광원은 반사하고 형광은 투과시킴으로써 광원과 형광을 분리하는 다색거울, 광원을 대상물의 넓은 면적에 조사하고 형광을 대면적 카메라에 뿌려주는 스캔거울, 입사한 광원을 대상물에 조사하고 대상으로부터 나온 형광을 집광하여 다시 내보내는 현미경부, 나온 형광신호로부터 백그라운드를 제거하고 관측하는 검출부로 구성된다.
PCT/KR2012/007190 2011-09-06 2012-09-06 공초점 형광 현미경 WO2013036049A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/342,966 US9563046B2 (en) 2011-09-06 2012-09-06 Confocal fluorescence microscope

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110090022A KR101393514B1 (ko) 2011-09-06 2011-09-06 고감도 실시간 공초점 형광 현미경
KR10-2011-0090022 2011-09-06

Publications (2)

Publication Number Publication Date
WO2013036049A2 WO2013036049A2 (ko) 2013-03-14
WO2013036049A3 true WO2013036049A3 (ko) 2013-05-02

Family

ID=47832713

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/007190 WO2013036049A2 (ko) 2011-09-06 2012-09-06 공초점 형광 현미경

Country Status (3)

Country Link
US (1) US9563046B2 (ko)
KR (1) KR101393514B1 (ko)
WO (1) WO2013036049A2 (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3538941A4 (en) 2016-11-10 2020-06-17 The Trustees of Columbia University in the City of New York METHODS FOR FAST IMAGING OF HIGH RESOLUTION LARGE SAMPLES
JP2018169502A (ja) * 2017-03-30 2018-11-01 オリンパス株式会社 顕微鏡装置
CN108051909B (zh) * 2017-11-20 2023-11-21 中国计量大学 一种结合光镊功能的扩展焦深显微成像系统
US11231570B2 (en) 2018-04-30 2022-01-25 University Of Central Florida Research Foundation, Inc. Highly inclined swept tile (HIST) imaging apparatus, methods, and applications
CN109839397B (zh) * 2019-01-23 2021-04-09 中国科学院上海应用物理研究所 同步辐射共聚焦荧光实验装置中共聚焦微元尺寸测量方法
CN110596059B (zh) * 2019-09-05 2024-05-28 北京世纪桑尼科技有限公司 光学超分辨显微成像系统
KR102290325B1 (ko) 2019-12-23 2021-08-18 주식회사 리암솔루션 집광렌즈 모듈을 포함하는 형광현미경
CN113866970B (zh) * 2021-09-27 2023-06-20 熵智科技(深圳)有限公司 集成超分辨和共聚焦功能的显微镜系统及方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09304701A (ja) * 1996-05-14 1997-11-28 Nikon Corp 共焦点レーザ走査顕微鏡
JP2003029152A (ja) * 2001-07-13 2003-01-29 Olympus Optical Co Ltd 共焦点レーザ走査型顕微鏡
US20090323058A1 (en) * 2006-11-28 2009-12-31 Leica Microsystems Cms Gmbh Laser microscope with a physically separating beam splitter
KR101004800B1 (ko) * 2008-09-19 2011-01-04 고려대학교 산학협력단 공초점 현미경

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5587832A (en) * 1993-10-20 1996-12-24 Biophysica Technologies, Inc. Spatially light modulated confocal microscope and method
KR100891699B1 (ko) 2007-04-10 2009-04-03 광주과학기술원 7-아미노-4-((ｓ)-3-(4-플루오로페닐)-2-((r)-3-메틸-2-(5-메틸이소옥사졸-3-카복사미도)부탄아미도)프로판아미도)-7-옥소-2-헵테노에이트 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 바이러스성 질환의 예방 및 치료용 약학적 조성물

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09304701A (ja) * 1996-05-14 1997-11-28 Nikon Corp 共焦点レーザ走査顕微鏡
JP2003029152A (ja) * 2001-07-13 2003-01-29 Olympus Optical Co Ltd 共焦点レーザ走査型顕微鏡
US20090323058A1 (en) * 2006-11-28 2009-12-31 Leica Microsystems Cms Gmbh Laser microscope with a physically separating beam splitter
KR101004800B1 (ko) * 2008-09-19 2011-01-04 고려대학교 산학협력단 공초점 현미경

Also Published As

Publication number Publication date
US9563046B2 (en) 2017-02-07
WO2013036049A2 (ko) 2013-03-14
KR101393514B1 (ko) 2014-05-13
US20140218794A1 (en) 2014-08-07
KR20130026702A (ko) 2013-03-14

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