WO2013007021A1

WO2013007021A1 - Container for nucleic acid amplification reaction

Info

Publication number: WO2013007021A1
Application number: PCT/CN2011/077085
Authority: WO
Inventors: 苏城; 邓秉华
Original assignee: 瑞基海洋生物科技股份有限公司
Priority date: 2011-07-12
Filing date: 2011-07-12
Publication date: 2013-01-17
Also published as: CN103635569A; KR101810017B1; CA2841019C; EP2733198A4; EP2733198B1; CN103635569B; EP2733198A1; CA2841019A1; KR20140034918A

Abstract

The present invention provides a container for a nucleic acid amplification reaction, comprising a capillary (100) and a heat conduction sleeve (200). The heat conduction sleeve (200) is tightly fitted on the outer side of the capillary (100) for heating the capillary (100) evenly when heat is transferred to the heat conduction sleeve (200), so that the capillary (100) is heated uniformly. In this way, the speed of the nucleic acid amplification reaction is increased.

Description

Nucleic acid amplification reaction container

TECHNICAL FIELD The present invention relates to nucleic acid amplification reactions, and more particularly to a container for use in a nucleic acid amplification reaction. BACKGROUND ART A nucleic acid amplification reaction is a technique in which a nucleic acid can be amplified by repeatedly using the same procedure and combining a specific polymerase. Common polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (RT-PCR), real-time polymerase chain reaction (real-time polymerase chain reaction, real -time PCR), all belong to the nucleic acid amplification reaction technology. Among them, the polymerase chain reaction refers to a technique of amplifying a specific deoxyribonucleic acid fragment. The reverse transcription polymerase chain reaction refers to a technique in which a deoxyribonucleic acid (DNA) is first transcribed by a messenger ribonucleic acid (mRNA), and the aforementioned polymerase chain reaction is carried out using the deoxyribonucleic acid. Real-time polymerase chain reaction refers to the technique of semi-quantitative detection using fluorescent probes or dyes during the polymerase chain reaction, also known as quantitative polymerase chain reaction (quantitative PCR;). Polymerase chain reaction techniques must be used in all of the aforementioned techniques. In addition, some novel technologies, such as Rolling Circle Amplification (RCA), Loop Mediated Isothermal Amplification (LAMP), Nucleic Acid Sequence Based amplification (Nucleic Acid Sequence Based Amplification, NASBA), Three Way Junction (TWJ), must also use polymerase chain reaction technology. When the polymerase chain reaction is carried out, the deoxyribonucleic acid and the primer are mixed in a buffer solution, and the double strand of the deoxyribonucleic acid is separated by a temperature of about 90 degrees Celsius; then, the primer is adhered at a temperature of about 50 degrees Celsius. The specific position of the deoxyribonucleic acid; the temperature of about 70 degrees Celsius is used to extend the primer attached to the deoxyribonucleic acid. This step is repeated to replicate a specific DNA fragment. The devices currently used to carry out the aforementioned heating process are of various types depending on the price. One of the less expensive types is to provide heating means at both ends of the container (usually a test tube), one of which is fixedly heated to 90 degrees Celsius and the other heated to 50 degrees Celsius. The solution in the container causes convection due to temperature differences, and the DNA and primers in the solution are circulated between 90 degrees Celsius and 50 degrees Celsius for polymerase chain reaction. However, conventional heating devices, which are usually metal blocks, have grooves for the container to be placed, and the shape of the grooves conforms to the container. When the container is placed on the heating device, the temperature of the heating device is raised to an appropriate temperature. Heat the container. The disadvantage is that the groove cannot be completely conformed to the container in actual production; that is, the groove has a small portion protruding and a small portion concave. The protruding part will cause the surrounding part to be in contact with the container, and the recessed part will make it impossible to contact the container. Thus, the container cannot be uniformly heated, affecting the polymerase chain reaction, that is, the reaction speed of the nucleic acid amplification reaction. SUMMARY OF THE INVENTION One aspect of the present invention is to provide a container for a nucleic acid amplification reaction that utilizes a close fitting technique to uniformly heat a container. According to one embodiment of the invention, a container for a nucleic acid amplification reaction comprises a capillary tube and a thermally conductive sleeve. The heat conducting sleeve is sleeved on the outside of the capillary for uniformly supplying thermal energy to the capillary. A container for a nucleic acid amplification reaction according to the present invention, wherein the heat conductive sleeve is a ring body. A container for a nucleic acid amplification reaction according to the present invention, wherein the heat conductive sleeve is a fastener. A container for a nucleic acid amplification reaction according to the present invention, wherein the heat conductive sleeve is C-shaped. A container for a nucleic acid amplification reaction according to the present invention, wherein the material shield of the capillary is plastic. A container for a nucleic acid amplification reaction according to the present invention, wherein the material shield of the capillary is polycarbonate. A container for a nucleic acid amplification reaction according to the present invention, wherein the material shield of the heat conductive sleeve is metal. A container for a nucleic acid amplification reaction according to the present invention, wherein the material shield of the heat conductive sleeve is iron. A container for a nucleic acid amplification reaction according to the present invention, wherein the capillary tube comprises a ring groove for accommodating and positioning the heat conductive sleeve. Accordingly, the capillary can be uniformly heated when the thermal energy is conducted to the thermal sleeve as compared with the prior art. BRIEF DESCRIPTION OF DRAWINGS FIG. 1 is a perspective view of a container according to an embodiment of the present invention. Figure 2 shows an exploded view of the container of Figure 1. FIG. 3 shows a cross-sectional view along AA of FIG. 1. 1 shows a perspective view of a container in accordance with an embodiment of the present invention. Figure 2 shows an exploded view of the container of Figure 1. As shown, the container contains a capillary tube 100 and a thermally conductive sleeve 200. The heat conducting sleeve 200 is sleeved on the capillary tube 100. FIG. 3 shows a cross-sectional view along line AA of FIG. 1. The foregoing heat conducting sleeve 200 is sleeved at one end of the capillary 100

110, this end 110 is a closed end. In use, the capillary tube 100 is placed in the recess 310 of the heat source 300, and the heat conducting sleeve 200 is heated by the heat source 300. By heat exchange, one end 110 of the capillary tube 100 can be heated by the heat energy supplied from the heat source 300. The temperature of one end 110 of the control capillary 100 is about 90 degrees Celsius, and the other end is cooled to about 50 degrees Celsius by ambient temperature to perform a nucleic acid amplification reaction in the capillary 100. Since the thermal sleeve 200 is sleeved outside the capillary 100, the thermal energy of the thermal sleeve 200 can be uniformly conducted to the capillary 100. Moreover, the capillary tube 100 is not in direct contact with the heat source 300, and there is no possibility of uneven heating. The heat conductive sleeve 200 is used to heat the capillary 100 uniformly, which can increase the reaction speed of the nucleic acid amplification reaction. Referring to Figure 2, the capillary 100 can include a ring groove 120. The ring groove 120 is located at the end 110 for receiving and positioning the heat conductive sleeve 200. The heat conducting sleeve 200 may be a ring body, and the inner diameter of the heat conducting sleeve 200 conforms to the outer diameter of the capillary tube 100, so that the heat conducting sleeve 200 can be tightly disposed outside the capillary tube 100. In addition, the heat conducting sleeve 200 can also be a fastener, and the inner diameter of the heat conducting sleeve 200 is less than or equal to the outer diameter of the capillary tube 100, so that the heat conducting sleeve 200 can be deformed tightly around the capillary tube 100. In detail, when the heat conductive sleeve 200 is a fastener, the shape of the heat conductive sleeve 200 may be C-shaped, that is, the heat conductive sleeve 200 may be a C-shaped buckle. In the technique in which the heat conducting sleeve 200 is sleeved in the capillary tube 100, when the heat conducting sleeve 200 is a ring body, the heat conducting sleeve

The inner side of the 200 is completely in contact with the outer side of the capillary 100. When the heat conductive sleeve 200 is a fastener, the inner side of the heat conductive sleeve 200 is completely in contact with the outer side of the capillary tube 100. The material shield of the capillary tube 100 is plastic. Further, the material shield of the capillary tube 100 is polycarbonate (PC). The material shield of the heat conductive sleeve 200 is metal. Further, the material shield of the heat conductive sleeve 200 is iron. The material shield of the capillary tube 100 and the heat-conductive sleeve 200 is a material shield which meets the demand and is relatively low in price according to the conditions of heat resistance and strength, and can surely reduce the product price.

Claims

A container for nucleic acid amplification reaction, comprising:

Capillary (100);

A thermal sleeve (200) is placed over the outside of the capillary (100). The container for nucleic acid amplification reaction according to claim 1, wherein the heat conductive sleeve (200) is a ring body. The container for nucleic acid amplification reaction according to claim 1, wherein the heat conductive sleeve (200) is a fastener. The container for nucleic acid amplification reaction according to claim 3, wherein the heat conductive sleeve (200) has a C shape. The container for nucleic acid amplification reaction according to claim 1, wherein the material shield of the capillary (100) is plastic. The container for nucleic acid amplification reaction according to claim 5, wherein the material shield of the capillary (100) is polycarbonate. The container for nucleic acid amplification reaction according to claim 1, wherein the material shield of the heat conductive sleeve (200) is metal. The container for nucleic acid amplification reaction according to claim 7, wherein the material shield of the heat conductive sleeve (200) is iron. The container for nucleic acid amplification reaction according to claim 1, wherein the capillary (100) comprises a ring groove (120) for accommodating and positioning the heat conductive sleeve (200) .