KR20140034918A - Container for nucleic acid amplification reaction - Google Patents

Container for nucleic acid amplification reaction Download PDF

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KR20140034918A
KR20140034918A KR1020147002886A KR20147002886A KR20140034918A KR 20140034918 A KR20140034918 A KR 20140034918A KR 1020147002886 A KR1020147002886 A KR 1020147002886A KR 20147002886 A KR20147002886 A KR 20147002886A KR 20140034918 A KR20140034918 A KR 20140034918A
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nucleic acid
acid amplification
container
amplification reaction
capillary tube
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KR1020147002886A
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Korean (ko)
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KR101810017B1 (en
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청 쑤
핑화 텅
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제네리치 바이오테크놀로지 코포레이션
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0858—Side walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

본 발명은 모세관(100)과 열전도 슬리브(200)를 포함하며, 열전도 슬리브(200)는 모세관(100)의 외측에 장착되며, 이를 통해 열 에너지가 열전도 슬리브에 전도되면, 모세관(100)을 균일하게 가열하여, 모세관(100)이 열을 균일하게 받게 함으로써, 핵산증폭반응의 반응속도를 향상시킬 수 있는 핵산증폭반응용 용기를 제공한다.The present invention includes a capillary tube 100 and a heat conducting sleeve 200, the heat conducting sleeve 200 is mounted on the outside of the capillary tube 100, through which the heat energy is conducted to the heat conducting sleeve, uniform the capillary tube 100 By heating so that the capillary tube 100 receives heat uniformly, the container for nucleic acid amplification reaction which can improve the reaction rate of a nucleic acid amplification reaction is provided.

Description

핵산증폭반응용 용기{CONTAINER FOR NUCLEIC ACID AMPLIFICATION REACTION}CONTAINER FOR NUCLEIC ACID AMPLIFICATION REACTION}

본 발명은 핵산증폭반응에 관한 것으로, 특히 핵산증폭반응용 용기에 관한 것이다. The present invention relates to a nucleic acid amplification reaction, and more particularly to a container for a nucleic acid amplification reaction.

핵산증폭반응이라 함은, 동일한 조작 절차를 반복적으로 이용하고, 특정된 중합효소를 결합하여, 핵산을 증폭시키는 기술을 가리킨다. 흔히 볼 수 있는 중합효소 연쇄반응(polymerase chain reaction, PCR), 역전사 중합효소 연쇄반응(reverse transcription polymerase chain reaction, RT-PCR), 실시간 중합효소 연쇄반응(real-time polymerase chain reaction, real-time PCR)은 모두 핵산증폭반응 기술에 속한다.Nucleic acid amplification reaction refers to a technique for amplifying a nucleic acid by using the same operation procedure repeatedly and binding to a specific polymerase. Common polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (RT-PCR), real-time polymerase chain reaction (real-time PCR) Are all nucleic acid amplification technology.

중합효소 연쇄반응이라 함은 특정 DNA 단편을 증폭시키는 기술을 가리킨다. 역전사 중합효소 연쇄반응이라 함은 mRNA 전사를 이용하여 DNA를 얻은 후, 이 DNA를 사용하여 상술한 중합효소 연쇄반응을 진행하는 기술을 가리킨다. 실시간 중합효소 연쇄반응이라 함은 중합효소 연쇄반응과정에서, 형광 탐침 또는 염료를 이용하여 반정량 검사를 하는 기술을 가리키며, 정량 중합효소 연쇄반응(quantitative PCR)이라고도 칭한다. 상술한 여러 가지 기술은 모두 중합효소 연쇄반응기술을 사용해야 한다.Polymerase chain reaction refers to a technique for amplifying a specific DNA fragment. The reverse transcriptase polymerase chain reaction refers to a technique of obtaining DNA using mRNA transcription and then proceeding the polymerase chain reaction described above using this DNA. Real-time polymerase chain reaction refers to a technique for semi-quantitative test using a fluorescent probe or dye in the polymerase chain reaction process, also referred to as quantitative PCR (quantitative PCR). All of the various techniques described above should use polymerase chain reaction technology.

또한, RCA (Rolling Circle Amplification), LAMP(Loop Mediated Isothermal Amplification), NASBA (Nucleic Acid Sequence Based Amplification), TWJ(Three Way Junction)와 같은 비교적 새로운 기술도 중합효소 연쇄반응기술을 사용해야 한다.In addition, relatively new technologies such as Rolling Circle Amplification (RCA), Loop Mediated Isothermal Amplification (LAMP), Nucleic Acid Sequence Based Amplification (NASBA), and Three Way Junction (TWJ) also require polymerase chain reaction technology.

중합효소 연쇄반응 시, DNA와 프라이머를 완충용액에 섞고, 약 90℃의 온도를 이용하여, DNA의 이중가닥(double strands)을 분리시키고; 이어서, 약 50℃의 온도를 이용하여, 프라이머를 DNA의 특정 위치에 부착시킨 다음; 약 70℃의 온도를 이용하여 DNA 상에 부착된 프라이머를 연장한다. 이렇게 절차를 반복하면 특정된 DNA 단편을 복제할 수 있다. In the polymerase chain reaction, DNA and primer are mixed in a buffer solution, and the double strands of DNA are separated using a temperature of about 90 ° C; Then, using a temperature of about 50 ° C., the primers were attached to specific positions of the DNA; A temperature of about 70 ° C. is used to extend the primer attached on the DNA. Repeating this procedure allows the specified DNA fragment to be replicated.

현재, 상술한 가열과정을 진행하는데 사용되는 장치는 가격에 따라 많은 종류가 있다. 그 중에서 비교적 저렴한 종류는 용기(통상적으로 시험관임) 양단에 가열장치가 설치되어 있는 것이고, 그 중, 하나의 가열장치는 90℃까지 가열하도록 고정하고, 다른 하나의 가열장치는 50℃까지 가열하도록 고정한다. 용기 중의 용액은 온도 차이로 인하여 대류가 발생하여, 용액 중의 DNA와 프라이머가 90℃와 50℃의 온도 사이에서 순환하도록 함으로써 중합효소 연쇄반응을 진행한다. At present, there are many kinds of apparatuses used for the above-described heating process depending on the price. Among them, a relatively inexpensive type is one in which a heating device is installed at both ends of a container (usually a test tube), and one heating device is fixed to heat up to 90 ° C, and the other heating device is heated to 50 ° C Fix it. Condensation occurs due to the temperature difference in the solution in the vessel, and the polymerase chain reaction proceeds by causing the DNA and the primer in the solution to circulate between temperatures of 90 ° C and 50 ° C.

그러나, 종래의 가열장치는 통상적으로 금속 블록이며, 상부에는 용기를 놓기 위한 오목홈이 있고, 오목홈의 형상은 용기와 매칭된다. 용기를 가열장치에 놓고 가열장치의 온도를 적절한 온도까지 상승시키면, 용기를 가열할 수 있다. 이러한 종류의 가열장치의 단점은 실제 제작에서 오목홈은 용기와 완전히 매칭될 수 없다는 것이다. 다시 말하면, 오목홈은 소부분이 돌출될 수도 있고, 함몰될 수도 있다는 것이다. 그 중 돌출 부분으로 인해 그 주변 부분이 용기와 접촉할 수 없게 되며, 함몰 부분으로 인해 함몰 부분에서 용기와 접촉할 수 없게 된다. 이렇게 되면 용기는 열을 균일하게 받지 못하여, 중합효소 연쇄반응, 즉 핵산증폭반응의 반응속도에 영향을 준다. However, the conventional heating apparatus is usually a metal block, and there is a recessed groove for placing the vessel on the top, and the shape of the recessed groove is matched with the vessel. The vessel can be heated by placing the vessel in a heating apparatus and raising the temperature of the heating apparatus to an appropriate temperature. The disadvantage of this kind of heating device is that in actual production the concave groove cannot be completely matched with the container. In other words, the concave grooves may be protruded or recessed. The protruding portion thereof does not allow the peripheral portion thereof to contact the container, and the recessed portion makes it impossible to contact the container at the recessed portion. As a result, the vessel is not uniformly subjected to heat, which affects the reaction rate of the polymerase chain reaction, that is, the nucleic acid amplification reaction.

본 발명의 기술 목적은, 밀접하게 끼워 맞춤 기술을 이용하여 용기가 균일하게 열을 받게 하는 핵산증폭반응용 용기를 제공하는 것이다. SUMMARY OF THE INVENTION The technical object of the present invention is to provide a nucleic acid amplification reaction vessel in which the vessel is uniformly heated using a closely fitting technique.

본 발명의 일 실시예에 따르면, 핵산증폭반응용 용기는 모세관과 열전도 슬리브를 포함한다. 열전도 슬리브는 모세관의 외측에 장착되어, 열 에너지를 모세관에 균일하게 제공한다. According to one embodiment of the invention, the nucleic acid amplification reaction container includes a capillary tube and a heat conducting sleeve. The heat conducting sleeve is mounted on the outside of the capillary tube to provide thermal energy uniformly to the capillary tube.

본 발명의 핵산증폭반응용 용기에 따르면, 열전도 슬리브는 환체이다.According to the container for nucleic acid amplification reaction of this invention, a heat conductive sleeve is a round body.

본 발명의 핵산증폭반응용 용기에 따르면, 열전도 슬리브는 파스너이다.According to the container for nucleic acid amplification reaction of the present invention, the heat conductive sleeve is a fastener.

본 발명의 핵산증폭반응용 용기에 따르면, 열전도 슬리브는 C형이다.According to the container for nucleic acid amplification reaction of this invention, a heat conductive sleeve is C type.

본 발명의 핵산증폭반응용 용기에 따르면, 모세관의 재질은 플라스틱이다.According to the container for nucleic acid amplification reaction of the present invention, the material of the capillary tube is plastic.

본 발명의 핵산증폭반응용 용기에 따르면, 모세관의 재질은 폴리카보네이트이다.According to the container for nucleic acid amplification reaction of the present invention, the material of the capillary tube is polycarbonate.

본 발명의 핵산증폭반응용 용기에 따르면, 열전도 슬리브의 재질은 금속이다.According to the container for nucleic acid amplification reaction of the present invention, the material of the heat conductive sleeve is metal.

본 발명의 핵산증폭반응용 용기에 따르면, 열전도 슬리브의 재질은 철이다.According to the container for nucleic acid amplification reaction of the present invention, the material of the heat conductive sleeve is iron.

본 발명의 핵산증폭반응용 용기에 따르면, 모세관은 환형 홈을 포함하며, 환형 홈은 열전도 슬리브를 수용 및 위치 고정시킨다.According to the container for nucleic acid amplification of the present invention, the capillary tube includes an annular groove, and the annular groove receives and positions the heat conducting sleeve.

본 발명에 의하면, 종래기술에 비해, 열 에너지가 열전도 슬리브에 전도되면, 모세관을 균일하게 가열할 수 있다. According to the present invention, when the thermal energy is conducted to the heat conducting sleeve as compared with the prior art, the capillary tube can be heated uniformly.

도 1은 본 발명의 일 실시예에 따른 용기를 나타낸 사시도이다.
도 2는 도 1의 용기를 나타낸 분해도이다.
도 3은 도 1의 A-A선에 따른 단면도이다.1 is a perspective view showing a container according to an embodiment of the present invention.
2 is an exploded view showing the container of FIG.
3 is a cross-sectional view taken along line AA of FIG. 1.

도 1은 본 발명의 일 실시예에 따른 용기를 나타낸 사시도이다. 도 2는 도 1의 용기를 나타낸 분해도이다. 도면에 도시한 바와 같이, 용기는 모세관(100)과 열전도 슬리브(200)를 포함한다. 그 중 열전도 슬리브(200)는 모세관(100)의 외측에 장착된다.1 is a perspective view showing a container according to an embodiment of the present invention. 2 is an exploded view showing the container of FIG. As shown in the figure, the container includes a capillary tube 100 and a heat conducting sleeve 200. The heat conducting sleeve 200 is mounted to the outside of the capillary tube 100.

도 3은 도 1의 A-A선에 따른 단면도이다. 상술한 열전도 슬리브(200)는 모세관(100)의 일단부(110)에 장착되고, 이 일단부(110)는 밀폐단이다. 사용 시, 모세관(100)은 열원(300)의 오목홈(310) 내에 수용되고, 열원(300)을 이용하여 열전도 슬리브(200)를 가열하고, 열 교환을 통하여 열원(300)이 제공하는 열 에너지를 이용하여 모세관(100)의 일단부(110)를 가열할 수 있다. 모세관(100)의 일단부(110)의 온도를 약 90℃로 제어하고, 타단부는 환경 온도를 이용하여 약 50℃까지 낮추면, 모세관(100) 내에서 핵산증폭반응을 진행할 수 있다.3 is a cross-sectional view taken along the line A-A of FIG. The heat conducting sleeve 200 described above is mounted to one end 110 of the capillary tube 100, and the one end 110 is a closed end. In use, the capillary tube 100 is received in the concave groove 310 of the heat source 300, heats the heat conducting sleeve 200 using the heat source 300, and heat provided by the heat source 300 through heat exchange. One end portion 110 of the capillary tube 100 may be heated using energy. When the temperature of one end 110 of the capillary 100 is controlled to about 90 ° C., and the other end is lowered to about 50 ° C. using an environmental temperature, the nucleic acid amplification reaction may be performed in the capillary 100.

열전도 슬리브(200)를 모세관(100)의 외측에 장착하므로, 열전도 슬리브(200)의 열 에너지가 모세관(100)에 균일하게 전도될 수 있다. 또한 모세관(100)은 열원(300)과 직접 접촉하지 않으므로 열을 균일하게 받지 못하는 상황이 발생하지 않는다. 열전도 슬리브(200)를 이용하여 모세관(100)이 열을 균일하게 받도록 하여, 핵산증폭반응의 반응 속도를 향상시킬 수 있다.Since the heat conduction sleeve 200 is mounted on the outside of the capillary tube 100, the heat energy of the heat conduction sleeve 200 may be uniformly conducted to the capillary tube 100. In addition, since the capillary 100 does not directly contact the heat source 300, a situation in which heat is not uniformly received does not occur. By using the heat conducting sleeve 200 to allow the capillary 100 to receive heat uniformly, the reaction rate of the nucleic acid amplification reaction can be improved.

도 2를 참고하면, 모세관(100)은 환형 홈(120)을 포함할 수 있다. 환형 홈(120)은 모세관(100)의 일단부(110)에 위치하고, 열전도 슬리브(200)를 수용 및 위치 고정시킨다.Referring to FIG. 2, the capillary tube 100 may include an annular groove 120. The annular groove 120 is located at one end 110 of the capillary 100 and accommodates and positions the heat conducting sleeve 200.

상기 열전도 슬리브(200)는 환체일 수 있으며, 열전도 슬리브(200)의 내경은 모세관(100)의 외경과 매칭되어, 열전도 슬리브(200)가 모세관(100) 외부에 장착될 수 있게 한다. 또한, 열전도 슬리브(200)는 파스너(fastener)일 수도 있으며, 열전도 슬리브(200)의 내경은 모세관(100)의 외경보다 작거나 같으며, 열전도 슬리브(200)가 변형하여 모세관(100)의 외부에 장착될 수 있게 한다. 상세하게는, 열전도 슬리브(200)가 파스너일 경우, 열전도 슬리브(200)의 형상은 C형일 수 있다. 즉, 열전도 슬리브(200)는 C형의 파스너일 수 있다.The thermally conductive sleeve 200 may be a ring, and the inner diameter of the thermally conductive sleeve 200 is matched with the outer diameter of the capillary tube 100 so that the thermally conductive sleeve 200 may be mounted outside the capillary tube 100. In addition, the heat conduction sleeve 200 may be a fastener, the inner diameter of the heat conduction sleeve 200 is less than or equal to the outer diameter of the capillary tube 100, the heat conduction sleeve 200 is deformed to the outside of the capillary tube 100 To be mounted on the In detail, when the heat conductive sleeve 200 is a fastener, the shape of the heat conductive sleeve 200 may be C-type. That is, the thermal conductive sleeve 200 may be a C-type fastener.

열전도 슬리브(200)를 모세관(100)에 장착하는 기술에서, 열전도 슬리브(200)가 환체일 경우, 열전도 슬리브(200)의 내측은 모세관(100)의 외측과 완전히 접촉된다. 열전도 슬리브(200)가 파스너일 경우, 열전도 슬리브(200)의 내측은 모세관(100)의 외측과 완전히 접촉된다.In the technique of mounting the heat conducting sleeve 200 to the capillary tube 100, when the heat conducting sleeve 200 is a torsion, the inside of the heat conducting sleeve 200 is in complete contact with the outside of the capillary tube 100. When the heat conducting sleeve 200 is a fastener, the inside of the heat conducting sleeve 200 is completely in contact with the outside of the capillary tube 100.

모세관(100)의 재질은 플라스틱이며, 더 나아가, 모세관(100)의 재질은 폴리카보네이트(polycarbonate, PC)이다. 열전도 슬리브(200)의 재질은 금속이며, 더 나아가, 열전도 슬리브(200)의 재질은 철이다. 상술한 모세관(100)과 열전도 슬리브(200)의 재질은, 내열 온도와 강도 등 조건에 따라 비교한 바에 의하면, 수요에 부합되고 비교적 저렴한 재질이므로, 제품의 가격을 확실히 낮출 수 있다.The material of the capillary 100 is plastic, and furthermore, the material of the capillary 100 is polycarbonate (PC). The material of the heat conductive sleeve 200 is metal, and furthermore, the material of the heat conductive sleeve 200 is iron. As the materials of the capillary tube 100 and the heat conducting sleeve 200 described above are compared according to conditions such as heat resistance temperature and strength, it is possible to lower the price of the product because it is a material that meets demand and is relatively inexpensive.

100: 모세관
200: 열전도 슬리브
110: 모세관의 일단부
300: 열원100: capillary
200: heat conducting sleeve
110: one end of the capillary
300: heat source

Claims (9)

모세관(100); 및
상기 모세관(100)의 외측에 장착하는 열전도 슬리브(200)
를 포함하는 핵산증폭반응용 용기. Capillary tube 100; And
Heat conducting sleeve 200 mounted on the outside of the capillary 100
Nucleic acid amplification reaction container comprising a. 제1항에 있어서,
상기 열전도 슬리브(200)는 환체(環體)인, 핵산증폭반응용 용기.The method of claim 1,
The heat conducting sleeve 200 is a container for a nucleic acid amplification reaction. 제1항에 있어서,
상기 열전도 슬리브(200)는 파스너(fastener)인, 핵산증폭반응용 용기.The method of claim 1,
The heat conducting sleeve 200 is a fastener, a nucleic acid amplification reaction container. 제3항에 있어서,
상기 열전도 슬리브(200)는 C형인, 핵산증폭반응용 용기.The method of claim 3,
The heat conducting sleeve 200 is C-type, nucleic acid amplification reaction vessel. 제1항에 있어서,
상기 모세관(100)의 재질은 플라스틱인, 핵산증폭반응용 용기.The method of claim 1,
The material of the capillary tube 100 is a plastic, nucleic acid amplification reaction vessel. 제5항에 있어서,
상기 모세관(100)의 재질은 폴리카보네이트(polycarbonate)인, 핵산증폭반응용 용기.6. The method of claim 5,
The capillary 100 is made of polycarbonate, a nucleic acid amplification container. 제1항에 있어서,
상기 열전도 슬리브(200)의 재질은 금속인, 핵산증폭반응용 용기.The method of claim 1,
The material of the heat conducting sleeve 200 is a metal, a nucleic acid amplification reaction container. 제7항에 있어서,
상기 열전도 슬리브(200)의 재질은 철(鐵)인, 핵산증폭반응용 용기.8. The method of claim 7,
The material of the heat conducting sleeve 200 is iron (鐵), a nucleic acid amplification reaction container. 제1항에 있어서,
상기 모세관(100)은 환형 홈(120)을 포함하며, 상기 환형 홈(120)은 상기 열전도 슬리브(200)를 수용 및 위치 고정시키는, 핵산증폭반응용 용기.The method of claim 1,
The capillary tube (100) includes an annular groove (120), wherein the annular groove (120) accommodates and positions the heat conducting sleeve (200), the container for nucleic acid amplification reaction.
KR1020147002886A 2011-07-12 2011-07-12 Container for nucleic acid amplification reaction KR101810017B1 (en)

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