TWM502686U - A container for nucleic acid amplification reaction - Google Patents

Abstract

A container for nucleic acid amplification reaction is disclosed. The container includes a capillary and a conductor. The conductor is tightly surrounded the capillary. The capillary can be heated evenly by the thermal energy when the thermal energy is conducted to the conductor.

Description

Nucleic acid amplification reaction container

The novel relates to nucleic acid amplification reactions, and in particular to a container.

The so-called nucleic acid amplification reaction refers to a technique in which a nucleic acid can be amplified by using the same operation procedure in combination with a specific polymerase. Common polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (RT-PCR), real-time polymerase chain reaction (real-time polymerase chain reaction, real- Time PCR), which belongs to the technology of nucleic acid amplification reaction.

Among them, the polymerase chain reaction refers to a technique of amplifying a specific DNA fragment. The reverse transcription polymerase chain reaction refers to a technique in which a ribonucleic acid (mRNA) is first transcribed to obtain a deoxyribonucleic acid (DNA), and the DNA polymerase chain reaction is carried out using the deoxyribonucleic acid. The instant polymerase chain reaction refers to the technique of semi-quantitative detection using fluorescent probes or dyes during the process of polymerase chain reaction, also known as quantitative polymerase chain reaction (quantitative PCR). In the foregoing several techniques, the technique of polymerase chain reaction must be used.

In addition, some of the more novel technologies, such as roller amplification (Rolling) Circle Amplification (RCA), Loop Mediated Amplification (LAMP), Nucleic Acid Sequence Based Amplification (NASBA), and Three Way Junction (TWJ) must also be used. The technique of polymerase chain reaction is also a nucleic acid amplification reaction or the like.

Wherein, for the polymerase chain reaction, when the reaction is carried out, the deoxyribonucleic acid and the primer are mixed in a buffer solution, and the double strand of the deoxyribonucleic acid is separated by using a temperature of about 90-100 degrees; At a temperature of about 50-60 degrees, the primer is attached to a specific position of the deoxyribonucleic acid; and the temperature of about 70 degrees is used to extend the primer attached to the DNA. This step is repeated to replicate specific DNA fragments.

The devices currently used to carry out the aforementioned heating process are of many types depending on the price. One of the less expensive types is to provide a heating device at both ends of a container (usually a test tube), wherein one heating device is fixedly heated to 90-100 degrees and the other heating device is fixedly heated to 50-60 degrees. The solution in the container will cause convection due to the temperature difference, and the DNA and the primer in the solution will circulate between the temperature differences for the polymerase chain reaction.

However, the conventional heating device is usually a metal block having a groove for the container to be placed, and the shape of the groove conforms to the container. The container can be heated when the container is placed on a heating device and the temperature of the heating device is raised to an appropriate temperature. The disadvantage is that the groove is not completely conformable to the container in actual production; that is, the groove has a small portion protruding and a small portion concave. The protruding part will cause the surrounding part to be in contact with the container, and the recessed part will make it impossible to contact the container. So there is no container The method is uniformly heated, affecting the polymerase chain reaction, that is, the reaction speed of the nucleic acid amplification reaction.

One of the technical aspects of the present invention is to provide a container for nucleic acid amplification reaction, which utilizes a tight fitting technique to uniformly heat the container.

According to an embodiment of the present invention, a container for nucleic acid amplification reaction comprises a capillary tube and a heat conducting sleeve. The thermal sleeve is pressed against the outside of the capillary to provide uniform heat to the capillary.

100‧‧‧ capillary

110‧‧‧

200‧‧‧heating sleeve

300‧‧‧heat source

120‧‧‧ Ring groove

Fig. 1 is a perspective view of a container according to an embodiment of the present invention.

Figure 2 is an exploded view of the container of Figure 1.

Figure 3 is a cross-sectional view taken along line 3-3 of Figure 1.

Fig. 1 is a perspective view of a container according to an embodiment of the present invention. Figure 2 is an exploded view of the container of Figure 1. As shown, the container includes a capillary tube 100 and a thermally conductive sleeve 200. The heat conducting sleeve 200 is pressed against the outside of the capillary 100.

Figure 3 is a cross-sectional view taken along line 3-3 of Figure 1. The aforementioned heat conducting sleeve 200 is pressed against one end 110 of the capillary 100, and this end 110 is a closed end. In use, the thermal sleeve 200 is heated by a heat source 300, and the heat of the heat source 300 can be utilized to heat the end 110 of the capillary 100. The temperature of the end of the capillary tube 100 is controlled to be about 90-100 degrees, and the other end is cooled to about 50-60 degrees by the ambient temperature to perform a nucleic acid amplification reaction in the capillary 100.

Since the thermal sleeve 200 is pressed outside the capillary 100, the thermal energy of the thermal sleeve 200 can be uniformly conducted to the capillary 100. Moreover, the capillary tube 100 is not in direct contact with the heat source 300, and there is no possibility of uneven heating. The capillary 100 is heated uniformly by the heat conducting sleeve 200, and the reaction speed of the nucleic acid amplification reaction can be increased.

Referring to Figure 2, the capillary 100 includes a ring groove 120. The ring groove 120 is located at the end 110 for receiving and positioning the heat conductive sleeve 200.

The heat conducting sleeve 200 can be a fastener, and the inner diameter of the heat conducting sleeve 200 is less than or equal to the outer diameter of the capillary tube 100, so that the heat conducting sleeve 200 can be deformed and pressed outside the capillary tube 100. In detail, when the heat conductive sleeve 200 is a fastener, the shape of the heat conductive sleeve 200 may be C-shaped, that is, the heat conductive sleeve 200 may be a C-shaped buckle. In addition, the heat conductive sleeve 200 may also be a ring body, and the inner diameter of the heat conductive sleeve 200 conforms to the outer diameter of the capillary tube 100, so that the heat conductive sleeve 200 can be pressed outside the capillary tube 100. In the present embodiment, the heat conductive sleeve 200 is exemplified by a fastener.

Wherein the heat conducting sleeve 200 is pressed in the technique of the capillary tube 100, the inner side of the heat conducting sleeve 200 can completely contact the outer side of the capillary tube 100 regardless of whether the heat conducting sleeve 200 is a fastener or a ring body.

The material of the capillary tube 100 is plastic. Further, the material of the capillary tube 100 is polycarbonate (PC). The material of the heat conductive sleeve 200 is metal. The material of the capillary tube 100 and the heat-conductive sleeve 200 is a material that meets the demand and is relatively inexpensive according to conditions such as heat resistance and strength, and can reliably reduce the product price.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention, and anyone skilled in the art can not deviate from the spirit of the present invention. And the scope of protection of the present invention is defined by the scope of the appended claims.

100‧‧‧ capillary

110‧‧‧

200‧‧‧heating sleeve

300‧‧‧heat source

120‧‧‧ Ring groove

Claims (7)

A container for nucleic acid amplification reaction comprising: a capillary tube; and a thermal sleeve that is pressed outside the capillary tube. A container for the nucleic acid amplification reaction of claim 1, wherein the thermally conductive sleeve is a ring. A container for the nucleic acid amplification reaction of claim 1, wherein the thermally conductive sleeve is a fastener. A container for the nucleic acid amplification reaction of claim 3, wherein the thermally conductive sleeve is C-shaped. The container for nucleic acid amplification reaction according to claim 1, wherein the capillary is made of plastic. The container for nucleic acid amplification reaction according to claim 5, wherein the capillary is made of polycarbonate. The container for nucleic acid amplification reaction according to claim 1, wherein the heat conductive sleeve is made of metal.