WO2012176782A1 - 電気泳動用チップおよびその製造方法 - Google Patents
電気泳動用チップおよびその製造方法 Download PDFInfo
- Publication number
- WO2012176782A1 WO2012176782A1 PCT/JP2012/065664 JP2012065664W WO2012176782A1 WO 2012176782 A1 WO2012176782 A1 WO 2012176782A1 JP 2012065664 W JP2012065664 W JP 2012065664W WO 2012176782 A1 WO2012176782 A1 WO 2012176782A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- gel
- support
- surface treatment
- electrophoresis chip
- electrophoresis
- Prior art date
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05D1/00—Processes for applying liquids or other fluent materials
- B05D1/36—Successively applying liquids or other fluent materials, e.g. without intermediate treatment
Definitions
- the present invention relates to an electrophoresis chip for separating biological samples and a method for producing the same.
- Electrophoresis is a phenomenon in which charged particles or molecules move an electric field, and is particularly important as a technique for separating DNA, proteins, etc. in molecular biology and biochemistry.
- proteome analysis has attracted attention as a post-genomic study.
- Proteome analysis is a study that analyzes the structure and function of proteins on a large scale. In order to perform proteome analysis, it is usually necessary to separate proteins contained in a sample into individual proteins. At this time, one of the commonly used techniques is two-dimensional electrophoresis.
- Two-dimensional electrophoresis is a technique for two-dimensionally separating proteins by two-stage electrophoresis.
- proteins are separated according to the isoelectric point by isoelectric focusing (IEF)
- sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE: Protein is separated according to molecular weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
- IEF isoelectric focusing
- SDS-PAGE Protein is separated according to molecular weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
- Such two-dimensional electrophoresis has a very high resolution and can separate each of thousands of proteins into spots.
- IEF immobilized pH gradient
- IPG gel immobilized pH gradient gel
- agarose gel or polyacrylamide gel is generally used.
- polyacrylamide gel it is common to use a homogeneous gel with a uniform acrylamide solution. However, if you want to observe a wide range of molecular weights, the concentration of the acrylamide solution is increased from low to high. In some cases, a gradient gel is used.
- IPG gels and SDS-PAGE gels are coated, for example, on plastic, glass or the like, or by pouring the gel solution into a mold (for example, between glass substrates opposed via a spacer). Can be formed.
- Patent Document 2 discloses an electrophoresis cell in which a gel material is filled after a hydrophilization treatment centering on a groove portion, and a manufacturing method thereof.
- the substrate surface is covered with a coating film composed of a cross-linked polymer after gelation, so that there is no sample leakage, a decrease in separation performance, a decrease in the SN ratio when performing optical detection, and the like.
- An electrophoresis cell can be produced.
- Patent Document 1 discloses a gel plate for electrophoresis using an inkjet method and a method for producing the same. According to Patent Document 1, it is possible to obtain an electrophoresis gel plate that allows a large amount of specimen sample to be simultaneously processed under the same conditions, and to produce a plurality of gel forming regions using an inkjet head. .
- Japanese Patent Publication Japanese Unexamined Patent Application Publication No. 2004-77393 (published on March 11, 2004)” Japanese Patent Publication “JP 2000-214132 A (published on August 4, 2000)”
- Patent Document 1 describes that the gel is covered with a coating film composed of a crosslinked polymer, but a complicated process is required for forming the coating film. Patent Document 2 also describes that the support is hydrophilized, but the affinity and the adhesive strength are insufficient in the hydrophilization.
- the present invention has been made in view of the above problems, and a main object is to provide a technique for easily and appropriately holding a gel in an electrophoresis chip.
- an electrophoresis chip is an electrophoresis chip including a gel made of a polymer obtained by polymerizing a monomer and a support that supports the gel. The surface of the support in contact with the gel is coated with a surface treatment compound containing the monomer or a derivative of the monomer.
- the adhesive force between the gel and the support can be strengthened, and the affinity between the gel and the support can be improved. That is, by covering the support with the monomer constituting the gel or a derivative thereof, the physical adhesive force between the gel and the support is more than that obtained by simply hydrophilizing the support.
- the affinity between the gel and the support can be improved as compared with the case where the support is simply hydrophilized. Thereby, detachment
- a gel can be suitably formed by radicalizing the surface treatment compound and polymerizing the monomer deposited on the support using the surface treatment compound as a trigger.
- the gel can be easily and appropriately held in the electrophoresis chip.
- a method for producing an electrophoresis chip according to the present invention is a method for producing an electrophoresis chip comprising a gel made of a polymer obtained by polymerizing monomers, and a support for supporting the gel, A coating step of coating at least a part of the surface of the support with a surface treatment compound containing the monomer or a derivative of the monomer; and on a coated region on the surface of the support; And a gel forming step for forming the gel.
- the adhesive force between the gel and the support can be strengthened, and the affinity between the gel and the support can be improved. That is, by covering the support with the monomer constituting the gel or a derivative thereof, the physical adhesive force between the gel and the support is more than that obtained by simply hydrophilizing the support.
- the affinity between the gel and the support can be improved as compared with the case where the support is simply hydrophilized. Thereby, detachment
- an electrophoresis chip that easily and appropriately holds a gel can be suitably manufactured.
- the adhesive force between the gel and the support can be strengthened, and the affinity between the gel and the support can be improved. Therefore, the gel can be easily and appropriately held in the electrophoresis chip.
- an electrophoresis chip refers to a chip on which a gel for electrophoresis is formed on a support, and refers to a chip that can be used for gel electrophoresis.
- the substance to be separated by electrophoresis using the electrophoresis chip according to the present invention may be any substance that is to be separated or analyzed by electrophoresis and transfer.
- a biological material for example, a living organism, body fluid, cell line
- polypeptides or polynucleotides can be more suitably used.
- FIG. 1 is a schematic diagram showing a schematic configuration of an electrophoresis chip 10 according to an embodiment of the present invention.
- the electrophoresis chip 10 is an electrophoresis chip including a gel 9 made of a polymer obtained by polymerizing monomers, and a base material (support) 1 that supports the gel 9.
- the surface in contact with the upper gel 9 is covered with the surface treatment compound 4 containing the monomer or a derivative of the monomer.
- the gel means a solid that loses fluidity because the monomer is polymerized and has a network structure by crosslinking.
- a derivative means an organic compound that has been modified without greatly changing its structure by introducing, changing, substituting, oxidizing, or reducing a functional group to the monomer.
- the adhesive force between the gel 9 and the substrate 1 can be strengthened, and the affinity between the gel 9 and the substrate 1 can be improved. That is, by covering the base material 1 with the monomer constituting the gel 9 or a derivative thereof, the physical adhesive force between the gel 9 and the base material 1 is simply hydrophilized.
- the affinity between the gel 9 and the base material 1 can be improved as compared with the case where the base material 1 is simply hydrophilized. Thereby, detachment
- a covalent bond can be formed between the surface treatment compound 4 and the gel 9 and can be bonded very firmly.
- the gel 9 can be suitably formed by radicalizing the surface treatment compound 4 and polymerizing the monomer deposited on the substrate 1 using the surface treatment compound 4 as a trigger. .
- the gel 9 can be easily and appropriately held in the electrophoresis chip 10. Details of each part will be described below.
- the substrate 1 is not particularly limited as long as it supports the gel.
- a film-like or flat substrate can be used.
- FIG. 2 is a schematic diagram showing a schematic configuration of the film-like substrate 1.
- the material for the film-like substrate 1 is not limited to this, and for example, polyester such as polyethylene terephthalate (PET), polyethylene, polypropylene, polyvinyl chloride, or the like can be used. Moreover, what is necessary is just to set the film thickness of the base material 1 suitably according to a use, and it is not specifically limited.
- FIG. 2 is a schematic diagram showing a schematic configuration of the flat substrate 1 ′.
- the material of the flat substrate 1 is not limited to this, but, for example, an acrylic plate such as polymethyl methacrylate (PMMA), a flat plate such as polycarbonate, polyvinyl chloride, polypropylene, glass, ceramics, and semiconductor substrate. Can be used.
- the substrate 1 is preferably provided with a gel adhesion region 2 that is a region where the gel 9 is to be formed.
- a gel adhesion region 2 that is a region where the gel 9 is to be formed.
- the substrate 1 is in the form of a film, it is preferably provided on the film surface of the substrate 1 as shown in FIG.
- the base material 1 when the base material 1 is flat, it may be provided on the plate surface of the base material 1, but as shown in FIG. It may be provided.
- the gel adhesion region 2 may be provided on a part of one surface of the substrate 1 or may be provided on the entire surface of the substrate 1.
- the shape of the gel adhesion region 2 is not particularly limited, and may be an appropriate shape according to the electrophoresis to be performed. Typically, the shape is a rectangle as shown in FIG. It may be a strip shape as shown in (b).
- the gel adhesion region 2 can be formed, for example, by masking the surface of the base material 1 in contact with the gel so as to surround the region to be the gel adhesion region 2.
- a predetermined shape of polyimide tape may be formed and attached to the substrate 1.
- a resist, a metal mask, or the like may be formed in a predetermined shape by using a photolithography method.
- the masking method is not particularly limited, and may be appropriately selected depending on the application.
- a masking process is not required.
- the base material 1 you may comprise so that a groove
- the gel 9 is formed so as to protrude from the base material 1.
- the gel 9 so as to protrude from the base material 1
- the electrophoresis chip 10 since the affinity and adhesion amount between the gel 9 and the base material 1 are strong, the gel 9 is formed so as to protrude from the base material 1. Also, the gel 9 can be appropriately retained.
- gel As the gel 9, a gel used for two-dimensional electrophoresis can be used.
- examples of gels used for two-dimensional electrophoresis include agarose gels and polyacrylamide gels. In particular, in recent years, polyacrylamide gels have been widely used.
- polyacrylamide gel examples include SDS-PAGE gel (second-dimensional gel) composed of acrylamide / bisacrylamide, and IPG gel composed of acrylamide / bisacrylamide and acrylamide derivatives.
- agarose gel for example, a polysaccharide in which D-galactose and 3,6-anhydro-L-galactose are alternately bonded can be used.
- a substituent may be introduced into D-galactose or 3,6-anhydro-L-galactose.
- the gel 9 may appropriately include a reagent for performing electrophoresis, a buffer solution, a reagent for storage, and the like.
- the surface treatment compound 4 contains a monomer constituting the polymer constituting the gel 9 or a derivative of the monomer.
- the gel 9 is a polyacrylamide gel
- the main skeleton of the gel 9 is formed from acrylamide. Therefore, the effects of the present embodiment can be obtained by using acrylamide or a derivative having a chemical structure similar to acrylamide as the surface treatment compound 4.
- suitable surface treatment compounds 4 include, but are not limited to, acrylamide, acrylic acid, methacrylic acid, 2-hydroxyethyl acrylate, 2-hydroxyethyl methacrylate, N-vinyl-2-pyrrolidone, acrylic And vinyl compounds such as acid-2- (N, N-dimethylamino) ethyl and methacrylic acid-2- (N, N-dimethylamino) ethyl.
- the chemical structure preferably has a structure represented by the structural formula CH 2 ⁇ CH—CO (NR 1 R 2 ) (where R 1 and R 2 are any independent functional groups).
- FIG. 3 is a schematic diagram showing a schematic configuration of the substrate 1 coated with the surface treatment compound 4 having a structure represented by the structural formula CH 2 ⁇ CH—CO (NR 1 R 2 ).
- the surface treatment compound 4 is provided on the gel adhesion region 2 of the substrate 1.
- H is desorbed from the acrylamide derivative (CH2 ⁇ CH—CO (NR 1 R 2 )), and the substrate —CH ⁇ CH—CO
- the structure can be (NR 1 R 2 ).
- the acrylamide constituting the gel 9 and the surface treatment compound 4 have a similar structure. Improves. Further, the vinyl group of the vinyl compound is cleaved to become a radical, and acrylamide constituting the polyacrylamide gel is polymerized in a chain manner using the radical as a reaction center. Thereby, the surface treatment compound 4 and the gel 9 are covalently bonded and firmly bonded.
- the gel 9 is an agarose gel
- the main skeleton of the gel 9 is formed from D-galactose and 3,6-anhydro-L-galactose, galactose, anhydrogalactose, and galactose or anhydrogalactose
- a derivative for example, monosaccharide
- the galactose constituting the gel 9 and the surface treatment compound 4 have a similar structure. Will improve. Furthermore, the surface treatment compound 4 and the gel 9 are covalently bonded and firmly bonded by forming a covalent bond accompanied by a dehydration reaction between the hydroxy group of the monosaccharide and the hydroxy group of the agarose gel.
- wet process such as inkjet method, dipping method, spin coating method, silane coupling method, screen printing method, Langmuir-Blodgett method, vapor deposition method, plasma weight
- the process can be appropriately selected from legal dry processes and the like, but is not limited thereto.
- FIG. 4 is a cross-sectional view illustrating each step of the method for manufacturing the electrophoresis chip 10.
- a method for manufacturing the electrophoresis chip 10 will be described with reference to FIG.
- a base material 1 is prepared.
- a gel adhesion region 2 is provided in advance on the substrate 1.
- the method for forming the gel adhesion region 2 can appropriately use a masking tape, a resist using a photolithography method, a metal mask, or the like.
- a masking tape for example, when a 70 mm ⁇ 13 mm polyethylene terephthalate film is used as the base material 1, a polyimide tape provided with a 50 mm ⁇ 2.4 mm punch pattern (window) by a laser processing machine or the like is used as the base material 1. You may form the gel adhesion area
- a thin film of the surface treatment compound 4 is formed on the gel adhesion region 2 of the substrate 1.
- a method of forming a thin film of the surface treatment compound 4 for example, a method of forming a thin film of the surface treatment compound 4 by injecting a solution of the surface treatment compound 4 onto the gel adhesion region 2 can be used.
- the injection of the surface treatment compound 4 solution can use an inkjet head, a sprayer or the like, and the substrate 1 may be dipped into the surface treatment compound 4 solution.
- graft polymerization can also be used as a method for coating the surface of the substrate 1 with the surface treatment compound 4. By coating using graft polymerization, variations in coating film can be reduced.
- a wet process such as a spin coating method, a silane coupling method, a screen printing method, a Langmuir-Blodgett method, or a dry process such as a vapor deposition method or a plasma polymerization method can be selected as appropriate, and even when these methods are used.
- the surface treatment compound 4 can be suitably coated on the surface of the substrate 1.
- a gel 9 is formed on the thin film of the surface treatment compound 4 (on the gel adhesion region 2).
- examples of the gel forming solution 8 include acrylamide mixed solution (acrylamide + N, N′-methylenebisacrylamide), Tris-HCl buffer (Tris-HCl), ammonium persulfate (APS: Ammonium presulfate) or tetramethylethylenediamine (TEMED: N, N, N ′, N′-tetramethylethylenediamine) can be used.
- the acrylamide mixed solution is a mixed solution of acrylamide that forms the main skeleton of the gel 9 and N, N′-methylenebisacrylamide that cross-links the main skeleton of the gel 9.
- APS is a polymerization initiator for acrylamide
- TEMED is a polymerization accelerator.
- a mixture of agarose and TBE or TAE buffer can be used as the gel-forming solution 8.
- an ejection density difference pattern (gradient pattern) of fine droplets 6 of the gel-forming solution 8 is formed and ejected in the direction indicated by the arrow in FIG.
- An immobilized pH gradient (IPG) gel can also be formed in the same manner.
- an IPG gel is formed when a monomer / buffer constituting the gel is formed into an acidic side gel forming solution and a basic side gel forming solution at a desired mixing ratio, and a gradient pattern in which the density is changed is used. It becomes possible.
- the gel 9 need not be formed as a gradient gel, and may be formed as a gel having a uniform concentration. Moreover, it is not necessary to use the inkjet head 20 for formation of the gel 9, and other methods (for example, the method of arrange
- the affinity with the gel 9 and the gel forming solution 8 is higher than the surroundings. Therefore, the gel 9 can be successfully formed in the gel adhesion region 2.
- the gel 9 is completed.
- the polyacrylamide gel is formed using APS and TEMED, and left to stand at room temperature (preferably 50 ° C.) and in a nitrogen atmosphere for about 1 to 3 hours. Is completed.
- the surface treatment compound 4 forms a very strong bond by being covalently bonded to the gel 9. This is because the vinyl group of the vinyl compound of the surface treatment compound 4 is cleaved to become a radical, and acrylamides are polymerized in a chain manner using this radical as a reaction center.
- an agarose gel can be gelled by hydrogen bonding.
- the gelation of agarose is due to cross-linking by a hydrogen bond between agarose, and can be performed by heating to about 60 ° C. to melt and cooling to room temperature, for example.
- an agarose gel is formed using an inkjet head, it is necessary to heat to about 60 ° C., and therefore it is preferable to use a heat-resistant inkjet head.
- the gel 9 is an agarose gel
- the gel 9 is completed by standing.
- the surface treatment compound 4 is covalently bonded to the gel 9 to form a very strong bond.
- the reason for this is that a covalent bond accompanied by a dehydration reaction is formed between the hydroxyl group of the monosaccharide of the surface treatment compound 4 and the hydroxyl group of the agarose gel.
- the gel 9 on the electrophoresis chip 10 is dried and stored in a refrigerator ( ⁇ 20 ° C. or lower) for long-term storage.
- the electrophoresis chip according to the present invention is an electrophoresis chip including a gel made of a polymer obtained by polymerizing a monomer and a support that supports the gel, The surface of the support in contact with the gel is covered with a surface treatment compound containing the monomer or a derivative of the monomer.
- the adhesive force between the gel and the support can be strengthened, and the affinity between the gel and the support can be improved. That is, by covering the support with the monomer constituting the gel or a derivative thereof, the physical adhesive force between the gel and the support is more than that obtained by simply hydrophilizing the support.
- the affinity between the gel and the support can be improved as compared with the case where the support is simply hydrophilized. Thereby, detachment
- a gel can be suitably formed by radicalizing the surface treatment compound and polymerizing the monomer deposited on the support using the surface treatment compound as a trigger.
- the gel can be easily and appropriately held in the electrophoresis chip.
- the gel is a polyacrylamide gel
- the surface treatment compound preferably contains a vinyl compound
- the polyacrylamide gel is useful as an electrophoresis medium used in two-dimensional electrophoresis or the like by applying a pH gradient, a concentration gradient, etc., and thus is suitable as a gel included in an electrophoresis chip.
- the surface treatment compound a vinyl compound which is a monomer constituting the polyacrylamide gel or a derivative thereof, the adhesive force between the gel and the support is strengthened, and the gel and the support The affinity between them can be improved.
- the gel is an agarose gel
- the surface treatment compound preferably contains a monosaccharide, and the monosaccharide is selected from the group consisting of galactose and anhydrogalactose. More preferably, it is a selected monosaccharide.
- agarose since agarose is useful as an electrophoresis medium, it can be suitably used as a gel provided in an electrophoresis chip.
- the surface treatment compound a monosaccharide that is a monomer constituting the agarose gel or a derivative thereof, the adhesion between the gel and the support is strengthened, and between the gel and the support. The affinity of can be improved.
- the surface treatment compound is preferably covalently bonded to the gel.
- the support and the gel can be bonded very firmly because the surface treatment compound is covalently bonded to the gel.
- the field of the polymerization activity is on a two-dimensional plane. Since it is obtained uniformly, the film quality of the gel can be improved.
- the gel is preferably formed so as to protrude from the support.
- the gel can be successfully formed so as to rise from the support.
- the gel is formed so as to protrude from the support, it is easy to apply the electrophoresis sample, and it is not necessary to form a special groove on the support. Can be simplified.
- a method for producing an electrophoresis chip according to the present invention is a method for producing an electrophoresis chip comprising a gel made of a polymer obtained by polymerizing monomers, and a support for supporting the gel, A coating step of coating at least a part of the surface of the support with a surface treatment compound containing the monomer or a derivative of the monomer; and on a coated region on the surface of the support; And a gel forming step for forming the gel.
- the adhesive force between the gel and the support can be strengthened, and the affinity between the gel and the support can be improved. That is, by covering the support with the monomer constituting the gel or a derivative thereof, the physical adhesive force between the gel and the support is more than that obtained by simply hydrophilizing the support.
- the affinity between the gel and the support can be improved as compared with the case where the support is simply hydrophilized. Thereby, detachment
- an electrophoresis chip that easily and appropriately holds a gel can be suitably manufactured.
- the monomer in the gel formation step, may be deposited on the region, and the monomer may be polymerized to form the gel.
- the monomer deposited on the support is polymerized by using the surface treatment compound as a trigger to form a gel. Therefore, the reaction field of polymerization is on a two-dimensional plane. Since it is obtained uniformly, the film quality of the gel can be improved.
- a gel forming solution for forming the gel in the gel forming step, may be discharged onto the region using an inkjet nozzle.
- a uniform electrophoresis chip can be easily produced with high productivity by using the ink jet printing technique.
- a gel having a pH gradient and a concentration gradient can be easily prepared.
- the surface treatment compound is graft-polymerized on the support in the coating step.
- the present invention can be used in the field of manufacturing analyzers such as biological samples.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Dispersion Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Graft Or Block Polymers (AREA)
Abstract
Description
本発明は、電気泳動用チップを提供する。本明細書において、電気泳動用チップとは、支持体上に電気泳動用のゲルが形成されているものを指し、ゲル電気泳動のために使用することができるものを指す。本発明に係る電気泳動用チップを用いた電気泳動の被分離物質としては、電気泳動および転写によって分離または分析すべき物質であればよく、例えば、生物材料(例えば、生物個体、体液、細胞株、組織培養物、または組織断片)からの調製物を好適に用いることができ、ポリペプチドまたはポリヌクレオチドをより好適に用いることができる。
基材1は、ゲルを支持するようになっていればよく、特に限定されないが、例えば、図2に示すように、フィルム状、平板状の基材を用いることができる。
基材1には、ゲル9を形成すべき領域であるゲル付着領域2が設けられていることが好ましい。例えば、基材1がフィルム状である場合には、図2の(a)に示すように、基材1のフィルム面に設けられていることが好ましい。また、例えば、基材1が平板状である場合には、基材1の板面に設けられていてもよいが、図2の(b)に示すように、基材1’の側端面に設けられていてもよい。また、ゲル付着領域2は、基材1の一面の一部に設けられていてもよいし、基材1の一面の全体に設けられていてもよい。
ゲル9としては、二次元電気泳動法に用いられるゲルを用いることができる。二次元電気泳動法に用いられるゲルとしては、アガロースゲル、ポリアクリルアミドゲル等がある。特に、近年、ポリアクリルアミドゲルが広く用いられている。
表面処理化合物4は、ゲル9を構成する高分子を構成する単量体または当該単量体の誘導体を含んでいる。
図4は、電気泳動用チップ10の製造方法の各工程を説明する断面図である。以下図4を参照して、電気泳動用チップ10の製造方法について説明する。
以上のように、本発明に係る電気泳動用チップは、単量体を重合してなる高分子からなるゲルと、該ゲルを支持する支持体とを備えた電気泳動用チップであって、該支持体上の該ゲルに接する面が、該単量体または該単量体の誘導体を含有している表面処理化合物によって被覆されていることを特徴としている。
2 ゲル付着領域
4 表面処理化合物
8 ゲル形成溶液
9 ゲル
10 電気泳動用チップ
20 インクジェットヘッド
Claims (11)
- 単量体を重合してなる高分子からなるゲルと、該ゲルを支持する支持体とを備えた電気泳動用チップであって、
該支持体上の該ゲルに接する面が、該単量体または該単量体の誘導体を含有している表面処理化合物によって被覆されていることを特徴とする電気泳動用チップ。 - 上記ゲルは、ポリアクリルアミドゲルであり、
上記表面処理化合物は、ビニル化合物を含有していることを特徴とする請求項1に記載の電気泳動用チップ。 - 上記ビニル化合物が、構造式CH2=CH-CONHR1(但し、R1は、-CH2CH2SO3H、-CHOHCOOH、-(CH2)nCH2COOH(n=1~3)、-(CH2)nC4H8ON(n=2もしくは3)、-(CH2)nC4H8SN(n=2もしくは3)、-(CH2)nNH2(n=2もしくは3)、-(CH2)3N(CH2CH3)2、または、-(CH2)3NH3 +を表す)、あるいは、構造式CH2=CHCOC4H9N2によって表される化合物であることを特徴とする請求項2に記載の電気泳動用チップ。
- 上記ゲルは、アガロースゲルであり、
上記表面処理化合物は、単糖類を含有していることを特徴とする請求項1に記載の電気泳動用チップ。 - 上記単糖類が、ガラクトースおよびアンヒドロガラクトースからなる群より選択される単糖であることを特徴とする請求項4に記載の電気泳動用チップ。
- 上記表面処理化合物が、上記ゲルと共有結合していることを特徴とする請求項1から5の何れか1項に記載の電気泳動用チップ。
- 上記ゲルは、上記支持体から隆起するように形成されていることを特徴とする請求項1から6の何れか1項に記載の電気泳動用チップ。
- 単量体を重合してなる高分子からなるゲルと、該ゲルを支持する支持体とを備えた電気泳動用チップの製造方法であって、
該支持体表面上の少なくとも一部を、該単量体または該単量体の誘導体を含有している表面処理化合物により被覆する被覆工程と、
該支持体表面上の被覆された領域上に、該ゲルを形成するゲル形成工程と、を含むことを特徴とする電気泳動用チップの製造方法。 - 上記ゲル形成工程では、上記単量体を上記領域上に堆積し、当該単量体を重合して上記ゲルを形成することを特徴とする請求項8に記載の電気泳動用チップの製造方法。
- 上記ゲル形成工程では、インクジェットノズルを用いて、上記ゲルを形成するためのゲル形成溶液を上記領域上に吐出することを特徴とする請求項8または9に記載の電気泳動用チップの製造方法。
- 上記被覆工程では、上記表面処理化合物を、上記支持体上にグラフト重合することを特徴とする請求項8から10の何れか1項に記載の電気泳動用チップの製造方法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14/127,614 US20140110261A1 (en) | 2011-06-21 | 2012-06-19 | Chip for electrophoresis and method for producing same |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2011-137849 | 2011-06-21 | ||
JP2011137849 | 2011-06-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2012176782A1 true WO2012176782A1 (ja) | 2012-12-27 |
Family
ID=47422614
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2012/065664 WO2012176782A1 (ja) | 2011-06-21 | 2012-06-19 | 電気泳動用チップおよびその製造方法 |
Country Status (3)
Country | Link |
---|---|
US (1) | US20140110261A1 (ja) |
JP (1) | JPWO2012176782A1 (ja) |
WO (1) | WO2012176782A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013128774A1 (ja) * | 2012-02-29 | 2013-09-06 | シャープ株式会社 | 等電点電気泳動用試験具およびその製造方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61296258A (ja) * | 1985-06-25 | 1986-12-27 | Fuji Photo Film Co Ltd | 電気泳動用媒体材料およびその製造法 |
JPH07128285A (ja) * | 1993-06-16 | 1995-05-19 | Hitachi Ltd | 電気泳動用支持体の作製法 |
WO2004031757A1 (ja) * | 2002-09-05 | 2004-04-15 | Katayanagi Institute | 物質の分離方法 |
JP2009042004A (ja) * | 2007-08-07 | 2009-02-26 | Norio Okuyama | 電気泳動用支持体 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5089103A (en) * | 1989-12-01 | 1992-02-18 | Hewlett-Packard Company | Electrophoresis capillary with agarose |
US5141612A (en) * | 1990-12-12 | 1992-08-25 | Studiengesellschaft Kohle Mbh | Production of polyacryamide gel filled capillaries for capillary gel electrophoresis |
JP2004077393A (ja) * | 2002-08-21 | 2004-03-11 | Seiko Epson Corp | 電気泳動用ゲルプレート及びその作製方法 |
JP4019967B2 (ja) * | 2003-02-19 | 2007-12-12 | 株式会社島津製作所 | 複数の電気泳動流路を備えた電気泳動装置 |
-
2012
- 2012-06-19 JP JP2013521590A patent/JPWO2012176782A1/ja active Pending
- 2012-06-19 WO PCT/JP2012/065664 patent/WO2012176782A1/ja active Application Filing
- 2012-06-19 US US14/127,614 patent/US20140110261A1/en not_active Abandoned
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61296258A (ja) * | 1985-06-25 | 1986-12-27 | Fuji Photo Film Co Ltd | 電気泳動用媒体材料およびその製造法 |
JPH07128285A (ja) * | 1993-06-16 | 1995-05-19 | Hitachi Ltd | 電気泳動用支持体の作製法 |
WO2004031757A1 (ja) * | 2002-09-05 | 2004-04-15 | Katayanagi Institute | 物質の分離方法 |
JP2009042004A (ja) * | 2007-08-07 | 2009-02-26 | Norio Okuyama | 電気泳動用支持体 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013128774A1 (ja) * | 2012-02-29 | 2013-09-06 | シャープ株式会社 | 等電点電気泳動用試験具およびその製造方法 |
Also Published As
Publication number | Publication date |
---|---|
JPWO2012176782A1 (ja) | 2015-02-23 |
US20140110261A1 (en) | 2014-04-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5840388A (en) | Polyvinyl alcohol (PVA) based covalently bonded stable hydrophilic coating for capillary electrophoresis | |
US20080017512A1 (en) | Coatings for capillaries capable of capturing analytes | |
WO1996023220A9 (en) | Polyvinyl alcohol (pva) based covalently bonded stable hydrophilic coating for capillary electrophoresis | |
RU2309959C1 (ru) | Применение немодифицированных полимерных материалов для изготовления подложки биочипов, биочип на их основе и способ его изготовления, способ иммобилизации гидрогелей на немодифицированных полимерных материалах | |
Wu et al. | Self-assembled epoxy-modified polymer coating on a poly (dimethylsiloxane) microchip for EOF inhibition and biopolymers separation | |
WO2011158520A1 (ja) | 電気泳動用反応器具の製造方法、電気泳動用反応器具の製造装置、ゲル固定用基材、電気泳動用反応器具及び電気泳動用キット | |
JP2001337088A (ja) | バイオチップ作製方法およびそれを用いたバイオチップ作製装置 | |
WO2012176782A1 (ja) | 電気泳動用チップおよびその製造方法 | |
US20080073511A1 (en) | Structured Copolymer Supports for Use in Mass Spectrometry | |
JP2010526301A (ja) | プロテオミクスのシステムと方法 | |
JP2000214132A (ja) | 電気泳動セル及びその製造方法 | |
US20140374260A1 (en) | Two-dimensional electrophoresis kit, method for manufacturing two-dimensional electrophoresis kit, two-dimensional electrophoresis method, and two-dimensional electrophoresis chip | |
US20060113189A1 (en) | Method for separating substances | |
KR20070031759A (ko) | 고분자겔을 이용한 바이오칩의 제조방법 | |
JP4851612B2 (ja) | 電気泳動用反応器具の製造方法及び電気泳動用反応器具の製造装置 | |
JPH07128285A (ja) | 電気泳動用支持体の作製法 | |
JPWO2013133083A1 (ja) | 電気泳動用ゲルの製造方法および電気泳動用ゲルの製造装置 | |
WO2013161368A1 (ja) | 等電点電気泳動用試験具およびその製造方法 | |
US20040069634A1 (en) | Vapor phase method for producing silane coatings | |
WO2013128772A1 (ja) | 等電点電気泳動用試験具およびその製造方法 | |
CN113358610A (zh) | 一种应用到微流控快检系统中硅基生物芯片及其封装工艺 | |
WO2013128774A1 (ja) | 等電点電気泳動用試験具およびその製造方法 | |
JP4851611B2 (ja) | ゲル固定用基材、電気泳動用反応器具、電気泳動用反応器具の製造方法及び電気泳動用キット | |
WO2013128777A1 (ja) | 等電点電気泳動用試験具およびその製造方法 | |
WO2014041908A1 (ja) | 電気泳動用試験具およびその製造方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 12803302 Country of ref document: EP Kind code of ref document: A1 |
|
ENP | Entry into the national phase |
Ref document number: 2013521590 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 14127614 Country of ref document: US |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 12803302 Country of ref document: EP Kind code of ref document: A1 |