WO2012169347A1 - Novel application of soybean emulsion composition to soybean-derived raw material-containing food or beverage - Google Patents

Novel application of soybean emulsion composition to soybean-derived raw material-containing food or beverage Download PDF

Info

Publication number
WO2012169347A1
WO2012169347A1 PCT/JP2012/063110 JP2012063110W WO2012169347A1 WO 2012169347 A1 WO2012169347 A1 WO 2012169347A1 JP 2012063110 W JP2012063110 W JP 2012063110W WO 2012169347 A1 WO2012169347 A1 WO 2012169347A1
Authority
WO
WIPO (PCT)
Prior art keywords
soybean
composition
soy
food
raw material
Prior art date
Application number
PCT/JP2012/063110
Other languages
French (fr)
Japanese (ja)
Inventor
佐本 将彦
金森 二朗
典史 足立
千鶴 植野
江里子 原田
真衣 神田
隆裕 鶴田
彩子 小蒲
佑希 臼井
斉藤 浩一
伊藤 公祐
菅野 秀夫
将志 淺野間
河野 光登
柴田 雅之
雄亮 宍戸
さゆり 北川
美友紀 金谷
芦田 茂
貴康 本山
Original Assignee
不二製油株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2011268484A external-priority patent/JP2013118841A/en
Priority claimed from JP2011268483A external-priority patent/JP5609854B2/en
Priority claimed from JP2011270843A external-priority patent/JP5488576B2/en
Priority claimed from JP2011271007A external-priority patent/JP5660018B2/en
Priority claimed from JP2011270860A external-priority patent/JP5585571B2/en
Application filed by 不二製油株式会社 filed Critical 不二製油株式会社
Priority to US14/123,837 priority Critical patent/US9101158B2/en
Priority to KR1020137034124A priority patent/KR101576797B1/en
Priority to CN201280027929.2A priority patent/CN103596451B/en
Publication of WO2012169347A1 publication Critical patent/WO2012169347A1/en

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C11/00Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/10Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
    • A23C11/103Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins containing only proteins from pulses, oilseeds or nuts, e.g. nut milk
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C20/00Cheese substitutes
    • A23C20/02Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates
    • A23C20/025Cheese substitutes containing neither milk components, nor caseinate, nor lactose, as sources of fats, proteins or carbohydrates mainly containing proteins from pulses or oilseeds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/40Pulse curds
    • A23L11/45Soy bean curds, e.g. tofu
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/50Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/60Drinks from legumes, e.g. lupine drinks
    • A23L11/65Soy drinks
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/66Proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/185Vegetable proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the present invention relates to a soy-derived raw material-containing food or drink using a soy material as a raw material in various applications. More specifically, the present invention relates to foods and beverages containing soybean-derived materials such as confectionery, breads, desserts, beverages, soups, sauces and the like using soybean-derived materials, and methods for producing them. Moreover, this invention relates to various foodstuffs, such as an acidic soybean raw material and western confectionery using the same. In particular, the present invention relates to an acidic soybean material suitable for uses similar to cheeses such as yogurts and fresh cheeses. The present invention also relates to an egg yolk substitute composition and a food using the same.
  • the present invention also relates to a processed soybean food such as tofu, a texture or flavor improving agent for manufacturing processed soybean food, and a method for improving the texture or flavor of processed soybean food.
  • a processed soybean food having a new texture and flavor, or a processed soybean food having improved conventional quality.
  • the present invention also relates to a composition for reducing serum cholesterol or improving renal function.
  • Soy materials such as soy milk, tofu and isolated soy protein are used as alternative raw materials for dairy products, alternative raw materials for fermented milk products, alternative raw materials for egg yolks, raw materials for processed soybean foods, raw materials for reducing serum cholesterol, and kidney function improvement It is used as a raw material in various food and drink applications such as raw materials for food.
  • Fresh cream and compound cream for topping (decoration), nappe (surface coating), filling, kneading, etc. for confectionery, bread, dessert, etc.
  • Fresh cream and compound cream for topping (decoration), nappe (surface coating), filling, kneading, etc. for confectionery, bread, dessert, etc.
  • Fresh cream and compound cream for topping (decoration), nappe (surface coating), filling, kneading, etc. for confectionery, bread, dessert, etc.
  • Fresh cream and compound cream for topping is widely used for imparting a unique, rich milk flavor and a cool mouthfeel.
  • fresh cream is an indispensable ingredient for pastry and cooking due to its rich body and milky taste, but it is relatively expensive and must be used within 2-3 days after opening even in refrigerated storage. Because it is difficult to store for a long time, and because the oil content is relatively high (30-50% by weight) and it is difficult to match healthy orientation, there is a need for a material that can replace fresh cream with good flavor and ease
  • tofu and soy milk is being considered as a material that can replace the above, but foods and beverages containing soy-derived ingredients such as confectionery, desserts, beverages, soups and sauces using such ingredients are healthy. Although there was a good image, it had a peculiar blue odor derived from soybeans, and it had poor body and umami. Therefore, the market needs for food and drink containing soybean-derived raw materials with improved flavor are high.
  • Patent Document 1 adds a low-sweet saccharide composition containing 3 to 4 saccharides having a branched structure to soybean foods and beverages, and is derived from soybeans.
  • a method for suppressing the blue odor of the heat treatment and suppressing the rough feeling produced in the heat treatment step is disclosed.
  • Patent Document 2 discloses a sterilization having a rich feeling (kokumi) and a good flavor characterized by including a step of allowing protein cross-linking enzyme to act on soy milk or a soybean raw material in the production process of soy milk, and then sterilizing. Soymilk is disclosed.
  • Patent Document 3 relates to an acidic soymilk beverage characterized in that soymilk is heat-treated at 130 to 150 ° C. for several tens of seconds and then acidified by lactic acid fermentation or addition of an acidic substance. There is disclosed a method for producing an acidic soy milk drink that has no unpleasant taste such as taste, has a refreshing mouth and has a rich taste.
  • fermented milk made by lactic fermentation of milk ingredients is eaten as cheeses and yoghurts as well as other Western confectionery, Japanese confectionery, bread, dessert, frozen confectionery, beverages, etc. It is used as a main ingredient in processed foods or as an auxiliary ingredient for topping (decoration), kneading, filling, nappe (surface coating).
  • topping decoration
  • kneading filling
  • nappe surface coating
  • skim milk powder or the like a filled milk type fermented emulsion produced by blending vegetable oil and fat in place of milk fat and subjecting it to lactic acid fermentation is known (Patent Documents 4 to 7). .
  • This emulsion is also used for the processed food in the same application as fermented milk, and imparts various functions and added values to the processed food, contributing to the development of the food industry.
  • fermented soy materials that use soy protein as a protein source do not use milk fat, so they are expected to be plant-based fermented materials that are good for health, and can be used as a substitute for general fermented dairy products such as cheese and yogurt.
  • soy materials such as soy milk, separated soy protein, and soy flour are used.
  • egg yolk has been widely used in various foods such as seasonings, sugar beets, and confectionery because it has excellent features such as function and nutritional value.
  • consumers' awareness of health has increased, and there is a tendency to avoid eating eggs with high cholesterol content.
  • chicken eggs that were originally said to be “price-preferred students” are concerned, however, due to the effects of disasters and avian infectious diseases in recent years, there are concerns about rising egg prices and unstable supply.
  • tofu, yuba and fried chicken made from soybeans are traditional processed soybean foods that have been eaten for a long time in Japan.
  • water is added to soybean and ground, and the resulting kure (soybean slurry) is heated as necessary, and the soymilk obtained by separating the okara by centrifugation or filtration is used as a starting material.
  • Various processed soybean foods are manufactured. Tofu is a typical example of processed soybean food, and various types of tofu are produced through a process of solidifying soy milk with a coagulant such as bittern.
  • a piece of curd curd that has been cut with a coagulant and exposed to water is called “silk tofu”, and then the card is dehydrated with a filter cloth while breaking down the card, and the molded one is called “cotton tofu” .
  • These tofu are relatively solid gels that have been sold as filled tofu sealed in plastic containers in recent years. This is common in that it has a texture of gel.
  • These traditional tofu have long been preferred and have become an indispensable part of the Japanese diet. On the other hand, they are differentiated from traditional tofu because they are industrially cheap and have low profit margins. New value-added tofu is eagerly desired.
  • Patent Document 13 describes a tofu paste obtained by mixing a thickening gelling agent, oligosaccharide or starch with tofu, stirring with a high-speed cutter, and heating and cooling.
  • Patent Document 14 describes a tofu-containing oil / fat composition obtained by adding tofu processed into a paste to the aqueous phase and emulsifying into a water-in-oil type.
  • soy milk is prepared from soybeans that have been heat-treated to reduce NSI, and this is acid precipitated to concentrate the protein, and further neutralized to redissolve the protein, and then add a coagulant.
  • soy milk is prepared from soybeans that have been heat-treated to reduce NSI, and this is acid precipitated to concentrate the protein, and further neutralized to redissolve the protein, and then add a coagulant.
  • a soybean protein curd having a cream-like texture is produced by heat coagulation.
  • Patent Document 16 is present as an application related to a composition for reducing serum cholesterol using soybean as a raw material. Further, Patent Document 17 exists as an application related to a blood cholesterol-lowering powder material. As an application related to the fractionated soybean protein material, Patent Document 18 exists. Patent Document 19 describes a soy protein material for nephropathy patients using soybean as a raw material.
  • Patent Document 1 has a slightly uncomfortable feeling that is unique to the low-sweet sugar composition and a natural soy flavor, and suppresses the blue odor derived from soybeans. Although it was possible, it was lacking in the rich body.
  • the method of Patent Document 2 in which a protein cross-linking enzyme is allowed to act on soy milk and then sterilized is a method that can improve both blue odor and body taste, but has a problem that delicate control of enzyme reaction conditions is necessary and is not easy. It was.
  • the acidic soymilk drink of patent document 3 does not have a blue odor and has a full-bodied taste
  • the usage is limited to beverages and cooking because of its acidic flavor.
  • none of the conventional methods can be said to be sufficient as a soy-derived material having a rich and rich taste without a blue odor derived from soy that can replace dairy products such as fresh cream.
  • the present invention provides a soy emulsified composition with improved flavor that can be used as a substitute for dairy products such as whipped cream, and a soy-derived raw material that does not have a blue odor derived from soy and has a rich body taste.
  • One of the problems is to provide the contained food and drink.
  • the present invention provides an acidic soy material that has a rich flavor like fermented milk and filled type fermented emulsions, has an unfavorable taste of soybean, and has a smooth texture.
  • Patent Document 9 can provide the rich taste required for egg yolk substitute foods only by blending pumpkin yellow particles instead of egg yolk. It wasn't.
  • a flavor with a blue odor derived from soy protein used as a raw material may affect the flavor of the product, and egg white protein is required. May be affected by instability.
  • the technology of Patent Document 11 attempts to reduce the unpleasant odor of soymilk by concentrating soymilk, it is not always satisfactory in flavor, and the quality of the mayonnaise-like food such as the richness is still not good. There was room for improvement.
  • an object of the present invention is to provide an egg yolk substitute composition that is rich and flavorful, is cheaper than egg yolk, and can be stably supplied, and a food using the same.
  • Patent Documents 13 and 14 both use traditional tofu obtained by a general manufacturing method, ground and processed into a paste.
  • Technology Since these are processed products using conventional tofu as they are, the flavor is only the flavor of the tofu itself, and the texture may be felt from the tofu gel.
  • the soybean protein card of Patent Document 15 requires a complicated process to make the tofu-like texture cream, and the soy milk is acid-precipitated so that it has a different flavor from the natural flavor of soybean. End up.
  • the present invention is a soy processed food having a new flavor and texture that is differentiated from conventional processed soybean foods such as tofu, or a processed soybean food having improved flavor and texture of conventional products. Is one of the issues.
  • the present invention has a composition for reducing serum cholesterol with improved flavor as one of the problems. Moreover, about the technique regarding the improvement of a renal function, this invention makes the composition for a renal function improvement by which flavor was improved one of the subjects.
  • the protein content per dry matter is 25% by weight or more and the lipid content (the content as a chloroform / methanol mixed solvent extract; the same applies in the following section) is 100% by weight or more with respect to the protein content.
  • soybean emulsion composition having an LCI value of 55% or more, or an acidic soybean material obtained by acidifying a raw material containing the soybean emulsion composition by lactic acid fermentation or acid addition Raw material-containing foods and beverages,
  • the soybean emulsified composition according to (1) wherein the dietary fiber content per dry matter is 10% by weight or less and the lipid content per dry matter is 35% by weight or more, or a raw material containing the soybean emulsified composition
  • a soy-derived raw material-containing food or drink comprising an acidic soy material obtained by acidification by lactic acid fermentation or acid addition
  • a dairy product or fermented dairy product substitute comprising the soybean emulsified composition or acidic soybean material according to (1) or (2) above
  • the dairy product or fermented dairy product substitute described in (3) above is used as a substitute for part or all of the dairy product or fermented dairy product, (5)
  • the present invention provides the following more specific solution means included in the above solution means in accordance with specific first to fifth problems.
  • ⁇ Invention of the present invention In view of the first problem related to dairy product replacement technology in foods, the present inventors have conducted various studies to obtain a soybean-derived material with improved flavor that can replace fresh cream, An insoluble fraction obtained by water extraction using processed soybean that has been modified in advance until the NSI reaches a specific range is obtained as a soybean emulsion composition in which neutral lipids and polar lipids are concentrated. Has been found to be useful as a soybean-derived material having a rich and rich taste without soybean-derived blue odor, and has completed the first invention.
  • the first invention provides the invention including the following (1) to (7).
  • a soy-derived raw material-containing food or drink comprising the present soybean emulsified composition
  • a soybean-derived material-containing food or drink comprising the soybean emulsion composition according to (1), wherein the dietary fiber content per dry matter is 10% by weight or less
  • a soybean-derived raw material-containing food or drink comprising the soybean emulsion composition according to (1), obtained by (4) The soy emulsified composition according to (3), which is obtained by further homogenizing the recovered insoluble fraction and then separating the solidified liquid to remove dietary fiber and recovering the supernatant.
  • Soy-derived raw material-containing food or drink characterized by containing foods characterized by containing foods
  • Use of the soybean emulsified composition according to any one of (1) to (4) above in a flavor-improved soybean-derived raw material-containing food or drink (6)
  • a method for improving the flavor of food-containing foods containing soybean-derived materials wherein the soybean emulsified composition according to any one of (1) to (4) is used as an active ingredient for flavor improvement
  • (7) A method for producing a food / beverage product containing a soybean-derived material, wherein the soybean emulsified composition according to any one of (1) to (4) is used as an active ingredient for flavor improvement.
  • This second invention In view of the second problem regarding alternative technology of fermented milk products in foods, the present inventors have conducted various studies using soy milk and separated soy protein as a soy material as a raw material. Even if a soybean material is used, it has been difficult to produce a flavor that has a rich body like milk and has an unfavorable flavor suppressed. In addition, it is difficult to obtain a smooth texture without the addition of other materials such as starch and polysaccharides. Therefore, the present inventors have changed their approach and are eagerly studying so that by using a soybean material having a specific unique composition as a raw material, it has a rich body, less unfavorable flavor, and smooth. An acidic soybean material having a texture was found. Furthermore, the obtained acidic soybean material was used in place of fermented milk, and confectionery and bread having a good flavor and texture were found, and the present second invention was completed.
  • the second invention provides the invention including the following (1) to (10).
  • An acidic soybean material obtained by acidifying a raw material containing the present soybean emulsified composition by lactic acid fermentation or acid addition, (2) The material according to (1), wherein the acidic soybean material is a cheese type or a yogurt type, (3) The material according to (1), wherein the acidic soybean material is a whey separation type obtained by separating the whey after acidification and collecting the card.
  • This third invention In view of the third problem related to egg yolk substitute technology in food, the present inventors have conducted intensive research, and as a result, the soybean emulsion composition has a rich and delicious egg yolk substitute composition. Obtaining the knowledge that it can be used in foods such as seasonings, confectionery, sauces, side dishes, etc., the present third invention has been completed.
  • the third invention provides inventions including the following (1) to (9).
  • An egg yolk substitute composition comprising the present soybean emulsified composition, (2) The egg yolk substitute composition according to (1), wherein one or more selected from the group of sugars, starches, spices, fats and oils, antioxidants and coagulants are added, (3) The egg yolk substitute composition according to the above (1) or (2), wherein a protein cross-linking enzyme is further acted on, (4) An egg yolk substitute food using the egg yolk substitute composition according to any one of (1) to (3), (5) The egg yolk substitute food according to the above (4), wherein the egg yolk substitute food is a seasoning, confectionery, sauces or sugar beet, (6) The egg yolk substitute food according to (5) above, wherein the seasoning is mayonnaise, dressings, sauces or dry seasonings, (7) The egg yolk substitute food according to (5), wherein the confectionery is a cream, a dessert or a baked confectionery, (8) Use of the present soybean emulsified composition as an egg yolk substitute composition, (9) A
  • the fourth invention provides inventions including the following (1) to (7).
  • Patent Document 16 In view of the fifth problem related to the technology for reducing serum cholesterol or the technology for improving renal function, the present inventors have conducted intensive research.
  • the action center of the composition for reducing serum cholesterol is 11S globulin, and fractionation is performed so as to contain a large amount thereof.
  • Patent Document 16 since a fraction containing a large amount of 11S globulin is prepared using defatted soybean as a starting material, a flavor derived from defatted soybean may be included.
  • Patent Document 17 describes a blood cholesterol-lowering powder material.
  • the inventor-derived emulsion composition prepared by a specific method without using defatted soybean as a starting material has a very good flavor, and It was found that it exhibits a strong serum cholesterol reducing action and a renal function improving action, and the fifth invention has been completed.
  • the fifth invention provides the invention including the following (1) to (5).
  • a composition for reducing serum cholesterol or improving renal function comprising the present soybean emulsified composition
  • (2) A composition for reducing serum cholesterol or improving renal function comprising the soybean emulsion composition according to (1), wherein the lipid is unrefined soybean oil
  • a composition for reducing serum cholesterol or improving renal function comprising the soybean emulsified composition according to (1) or (2), obtained by (4) A food using the composition for reducing serum cholesterol or improving renal function according to (1), (5) A method for producing a food using the composition for reducing serum cholesterol or
  • the present soybean emulsified composition enriched with neutral lipids and polar lipids obtained from fat-containing soybeans according to the first invention has no blue smell peculiar to soybeans, a rich body taste, and a flavor excellent in natural soybean taste
  • an acidic soy material that has very few undesirable flavors such as a deteriorated odor, an acetic acid odor, and an astringent taste that are felt when lactic fermentation of conventional soy milk or isolated soy protein.
  • this acidic soybean raw material can be used for the various foodstuffs in which cheese and yoghurt are utilized, and a part or all substitution of cheese and yoghurt is possible.
  • this acidic soy material in Western confectionery, if this acidic soy material is used as the main raw material for rare cheesecake as an alternative to cheese, it can be made inferior to that made with cheese, is rich, and is derived from soybeans. A rare cheesecake-like food with a refreshing and smooth texture can be obtained.
  • an egg yolk substitute composition containing the present soybean emulsified composition by using the egg yolk substitute composition containing the present soybean emulsified composition, an egg yolk substitute food that is creamy and rich and has a good flavor can be produced.
  • the soy emulsion composition is used as a raw material for various processed soybean foods, so that generally there is no blue smell characteristic of soybeans, a rich body taste and a natural soybean taste are imparted, and creamy A processed soy food with characteristics that are differentiated from conventional products in flavor and texture can be provided.
  • tofu and deep-fried food it is softer, creamier than conventional products, and has a mouthfeel that melts in the mouth.
  • Yuba it is easy to obtain a thick texture like freshly-cured Yuba (pumped Yuba).
  • puffed foods such as cancer pounding and deep-fried food can promote the expansion of dough raw materials when fried and cooked more than conventional products, and can give the effect of improving the puffing rate. And the expansion rate improves, The absorptive power of the seasoning liquid after cooking can also improve, and a more juicy food texture can be given.
  • the composition for reducing serum cholesterol or improving renal function according to the fifth invention has a good flavor and serum cholesterol reducing action or renal function improving action. By using this composition, the cholesterol reducing effect or renal function improving action is obtained. Various foods with excellent flavor and effects can be easily obtained.
  • the soybean-derived raw material-containing food and drink of the first invention, the egg yolk substitute composition of the third invention, the processed soybean food of the fourth invention, and the composition for reducing serum cholesterol or improving renal function of the fifth invention are any Is also characterized by containing a novel “soybean emulsified composition” described below.
  • the acidic soybean material of the second invention is also characterized by being obtained by acidifying a raw material containing the soybean emulsified composition by lactic acid fermentation or acid addition.
  • the soybean emulsified composition used in the present invention is derived from soybean, and has a particularly high ratio of lipophilic proteins other than glycinin and ⁇ -conglycinin (or lipoxygenase protein as another index) among proteins, neutral lipids and It is an emulsified composition containing a large amount of polar lipids. That is, the protein content per dry matter is 25% by weight or more, and the lipid content per dry matter (referred to as a chloroform / methanol mixed solvent extract) is 100% by weight or more with respect to the protein content per dry matter,
  • the main characteristic is that the LCI value (Lipophilic Proteins Content Index) is 55% or more, more preferably 60% or more.
  • the lipid content in the present invention is chloroform: methanol.
  • solvent extraction device “Soxtec” manufactured by FOSS can be used. The above measurement method is referred to as “chloroform / methanol mixed solvent extraction method”.
  • the present soybean emulsified composition contains a lipid having a value higher than the ratio of the lipid content / protein content of the soy flour, and is characterized by being particularly rich in polar lipids.
  • the lipid is a lipid derived from soybean as a raw material.
  • the lipid content of the present soybean emulsified composition is at least 100% by weight, preferably 120 to 250% by weight, more preferably 120 to 200% by weight, based on the protein content per dry matter. It is a feature. Although not essential for the constitution, when the lipid content is expressed in absolute amount, it is appropriate that it is 35% by weight or more, preferably 40% by weight or more per dry matter. If the soybean emulsified composition is one in which fibers and the like are removed, the lipid content can be 50% by weight or more per dry matter.
  • the upper limit of the lipid content is not limited, but is preferably 75% by weight or less, more preferably 70% by weight or less.
  • the protein content of the present soybean emulsified composition is 25% by weight or more, preferably 30% by weight or more per dry matter.
  • the upper limit of the protein content is not limited, but is preferably 50% by weight or less, more preferably 40% by weight or less.
  • the protein content in the present invention is measured as the amount of nitrogen by the Kjeldahl method, and is obtained by multiplying the amount of nitrogen by a nitrogen conversion factor of 6.25.
  • the component composition of the protein of this soybean emulsified composition can be analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE). Hydrophobic interactions, hydrogen bonds, and intermolecular disulfide bonds between protein molecules are cleaved by the action of the surfactant SDS and the reducing agent mercaptoethanol, and negatively charged protein molecules follow their intrinsic molecular weight. By showing the electrophoretic distance, it exhibits a migration pattern characteristic of proteins. After electrophoresis, after staining the SDS gel with Coomassie Brilliant Blue (CBB), calculate the ratio of the density of bands corresponding to various protein molecules to the density of all protein bands using a densitometer. It can be obtained by the method to do.
  • SDS-PAGE SDS polyacrylamide gel electrophoresis
  • This soybean emulsified composition is characterized in that it contains a specific amount or more of lipoxygenase protein that is generally not contained in the oil body in soybean, and contains at least 4% or more per total protein in the soybean emulsion composition. , Preferably 5% or more.
  • NKI 90 or higher normal undenatured soybeans
  • the lipoxygenase protein exists in a soluble state, and therefore when extracted with water, it is extracted to the water-soluble fraction side.
  • the lipoxygenase protein remains in the insoluble fraction side because it is inactivated and insolubilized in the raw soybean by heat treatment.
  • the increase in the proportion of lipoxygenase protein in the protein not only stabilizes the emulsified state of fats and oils, but also provides a smooth texture that cannot be obtained with a normal soy protein composition mainly composed of globulin proteins. In addition, a rich flavor is added to the material.
  • lipoxygenase protein there are usually three types, L-1, L-2 and L-3, and the content can be calculated from the intensity of these bands corresponding to the lipoxygenase protein by the above-mentioned electrophoresis method.
  • Lipophilic protein The present soybean emulsified composition is characterized in that among the types of proteins, lipophilic proteins are contained more than general soybean materials.
  • Lipophilic protein refers to a group of minor acid-precipitating soybean proteins other than glycinin (7S globulin) and ⁇ -conglycinin (11S globulin) among the major acid-precipitating soybean proteins of soybean, such as lecithin and glycolipids. It accompanies many polar lipids. Hereinafter, it may be simply abbreviated as “LP”.
  • the LP is a mixture of miscellaneous proteins, so it is difficult to identify each protein and measure the LP content precisely, but by calculating the following LCI (Lipophilic Proteins Content Index) value Can be estimated.
  • the LCI value of the protein in the present soybean emulsified composition is usually 55% or more, preferably 58% or more, more preferably 60% or more, further preferably 63% or more, and most preferably 65%. % Or more.
  • NAI 90 or higher normal undenatured soybeans
  • LP remains in the insoluble fraction side because LP is inactivated and insolubilized by heat treatment in the raw soybean.
  • the increase in the proportion of LP in the protein not only stabilizes the emulsified state of the fats and oils, but also provides a smooth texture that cannot be obtained with a normal soy protein composition mainly composed of globulin proteins.
  • a rich flavor is imparted to the material.
  • the soybean emulsified composition usually has a fresh cream-like property, and the usual dry matter is about 20 to 30% by weight, but is not particularly limited. That is, it may be a liquid having a low viscosity by water addition, a cream having a higher viscosity by concentration, or a powder processed to be a powder.
  • the soybean emulsified composition has a water-soluble nitrogen index (Nitrogen Solubility Index, hereinafter referred to as “NSI”) of 20 to 77, preferably 20 to 70, and a full fat soybean having a lipid content of 15% by weight or more per dry matter.
  • NAI water-soluble nitrogen index
  • the suspension is subjected to solid-liquid separation, neutral lipids and polar lipids are transferred to an insoluble fraction, and protein and sugar It can be obtained by removing the water-soluble fraction containing the quality and collecting the insoluble fraction.
  • this production mode will be described.
  • fat-containing soybeans such as full-fat soybeans or partially defatted soybeans are used.
  • partially defatted soybeans include those obtained by partially defatting whole fat soybeans by physical extraction treatment such as compression extraction.
  • fat is contained in about 20 to 30% by weight of dry matter in full fat soybeans, and special soybean varieties have lipids of 30% by weight or more, and are not particularly limited.
  • a material containing at least 15% by weight or more, preferably 20% by weight or more of lipid is suitable.
  • the form of the raw material may be in the form of half cracked soybeans, grits, powder.
  • soybean-emulsified composition rich in lipids used in the present invention.
  • defatted soybeans extracted with an organic solvent such as hexane and having a neutral lipid content of 1% by weight or less are not preferable because the good flavor of soybeans is impaired.
  • the NSI is usually over 90, but in the present invention, the NSI is 20 to 77, preferably 20 to 70. It is appropriate to use processed soybeans that have been treated.
  • a more preferable lower limit of NSI can be 40 or more, more preferably 41 or more, still more preferably 43 or more, and most preferably 45 or more.
  • a more preferable upper limit of NSI can be less than 75, more preferably less than 70, and a lower NSI of less than 65, alternatively less than 60, or less than 58 can be used.
  • Such processed soybeans are obtained by performing processing such as heat treatment or alcohol treatment.
  • the processing means is not particularly limited, and for example, heat treatment such as dry heat treatment, steam treatment, superheated steam treatment, microwave treatment, hydrous ethanol treatment, high pressure treatment, and combinations thereof can be used.
  • the ratio of the protein in the soybean emulsified composition tends to be high, and the lipid content to the protein is low. Moreover, miscellaneous taste such as roasting odor due to overheating tends to occur. Conversely, when the NSI is a high value of 80 or more, for example, the protein ratio in the soybean emulsified composition decreases, and the lipid recovery rate from soybean tends to decrease. In addition, the flavor has a strong blue odor.
  • the treatment conditions are influenced by the production environment, so it cannot be said unconditionally, but the NSI of processed soybeans is used for 5 to 10 minutes using superheated steam at approximately 120 to 250 ° C.
  • the processing conditions may be selected as appropriate so that the value falls within the above range, and no particular difficulty is required for the processing. For convenience, commercially available soybeans with NSI processed in the above range can also be used.
  • NSI can be expressed as a ratio (% by weight) of water-soluble nitrogen (crude protein) in the total amount of nitrogen based on a predetermined method.
  • NSI is a value measured based on the following method. . That is, 100 ml of water is added to 2.0 g of a sample, followed by stirring and extraction at 40 ° C. for 60 minutes, followed by centrifugation at 1400 ⁇ g for 10 minutes to obtain supernatant 1. 100 ml of water is added again to the remaining precipitate, followed by stirring and extraction at 40 ° C. for 60 minutes, and centrifugation at 1400 ⁇ g for 10 minutes to obtain supernatant 2.
  • Supernatant 1 and supernatant 2 are combined, and water is further added to make 250 ml.
  • the nitrogen content of the filtrate is measured by Kjeldahl method.
  • the nitrogen content in the sample is measured by the Kjeldahl method, and the ratio of the nitrogen recovered as filtrate (water-soluble nitrogen) to the total nitrogen in the sample is expressed as weight%, which is NSI.
  • the processed soybean is subjected to a tissue destruction treatment such as dry or wet pulverization, crushing, and pressure bias in advance before water extraction.
  • tissue destruction treatment it may be swollen in advance by water immersion or steaming, thereby reducing the energy required for tissue destruction, and eluting and removing components with unpleasant taste such as whey protein and oligosaccharides, as well as water retention
  • the extraction ratio of globulin proteins (especially glycinin and ⁇ -conglycinin) having a high ability and gelling ability to the total protein, that is, the transfer ratio to the water-soluble fraction can be further increased.
  • Water extraction from raw soybeans Water extraction is carried out by adding 3 to 20 times, preferably 4 to 15 times, the amount of water to fat-containing soybeans and suspending the fat-containing soybeans.
  • the higher the rate of hydrolysis the higher the extraction rate of the water-soluble component and the better the separation. However, if it is too high, concentration is required and costs increase. Further, when the extraction process is repeated twice or more, the extraction rate of the water-soluble component can be further increased.
  • the extraction temperature is not particularly limited. However, the higher the extraction rate of the water-soluble component, the more easily the oil and fat are solubilized, and the lower the lipid of the soybean emulsified composition. The following should be done. Alternatively, it can be carried out in the range of 5 to 80 ° C., preferably 50 to 75 ° C.
  • the higher the extraction pH (the pH of the soybean suspension after the addition of water), the higher the temperature, the higher the extraction rate of the water-soluble component, but the fats and oils also tend to be solubilized, and the lipid in the soybean emulsion composition tends to decrease.
  • the pH is too low, the protein extraction rate tends to be low.
  • the lower limit can be adjusted to pH 6 or higher, pH 6.3 or higher, or pH 6.5 or higher.
  • the upper limit can be adjusted to pH 9 or lower, pH 8 or lower, or pH 7 or lower from the viewpoint of increasing lipid separation efficiency.
  • Solid-liquid separation after water extraction After water extraction, the suspension of fat-containing soybeans is subjected to solid-liquid separation by centrifugation, filtration or the like. At this time, not only neutral lipids but also most of the lipids including polar lipids are not eluted in the water extract, but transferred to the insolubilized protein or dietary fiber to make the precipitation side (insoluble fraction). is important. Specifically, 70% by weight or more of the lipid of the fat-containing soybean is transferred to the precipitation side.
  • lipid in soy milk a small amount of lipid is also eluted on the supernatant side during extraction, but it is not finely emulsified like the lipid in soy milk, and it is centrifuged at 15,000 xg or less, or about 5,000 xg or less. In this respect, it is preferable to use a centrifuge.
  • the centrifugal separator can be used for ultracentrifugation of 100,000 ⁇ g or more, and in the case of the present soybean emulsified composition used in the present invention, an ultracentrifugal separator is not required. Implementation is possible.
  • the demulsifier is not particularly limited, but for example in Japanese application (Japanese Patent Application No. 2011-108598) Demulsifiers cited in Patent Document 2 described herein may be used. However, the preparation of the soybean emulsified composition can be carried out without using a demulsifier.
  • the obtained insoluble fractions (1) and (3) can be used as they are, or, if necessary, through the concentration step, the heat sterilization step, the powdering step, etc. to obtain the present soybean emulsified composition.
  • the obtained insoluble fraction contains dietary fiber, for example, if it is the fraction of (3) or (1) and (3) above, water is added if necessary, and a high-pressure homogenizer or jet cooker heater After homogenization by means of, etc., through the process of further solid-liquid separation of the homogenized liquid and collecting the supernatant, dietary fiber (ocara) can also be removed, and the rich flavor is further concentrated
  • This soybean emulsified composition can be obtained.
  • Proteins can be more easily extracted by adding a heat treatment step, an alkali treatment step or the like, if necessary, before or after the homogenization.
  • the dietary fiber content per dry matter is 10% by weight or less, and more preferably 5% by weight or less.
  • the dietary fiber content can be measured by the enzyme-weight method (modified Prosky method) according to the “Fifth Supplement Japanese Food Standard Ingredient Table” (Ministry of Education, Culture, Sports, Science and Technology, 2005).
  • the present soybean emulsified composition is an emulsified composition that contains lipids (neutral lipids and polar lipids) and proteins in a specific range, has a particularly high LP content of proteins, and also contains fibers as necessary.
  • the natural taste that is inherently concentrated is concentrated, and there is no or very little unpleasant taste such as blue odor, astringent taste, astringency, etc., which has been regarded as a conventional problem, and it has a very rich flavor. .
  • soybean emulsified composition prepared by this technique is characterized by remarkably good flavor and high suitability for use as a food material compared to such an assembled product.
  • the lipid in the present soybean emulsified composition is unrefined soybean oil. That is, the most preferable result can be obtained when the lipid in the present soybean emulsified composition is derived from the whole soybean, which is the raw material, and the present soybean emulsion prepared from the whole soybean by a predetermined method is, for example, It is most preferable as the action body of the composition for reducing serum cholesterol of the fifth invention.
  • the soy-derived raw material-containing food / beverage in the present invention is broadly defined as a milk substitute composition, an acidic soybean material, an egg yolk substitute composition, or a processed soybean food as described in the first to fifth inventions described later.
  • the present soybean emulsified composition used in the present first invention has the above-mentioned characteristics, and has no blue smell peculiar to soybeans and a unique richness compared to conventional tofu, soymilk and soy emulsified compositions.
  • the soy emulsified composition is widely used as a substitute for dairy products such as milk and fresh cream. It can be used for foods and drinks in which dairy products such as fresh creams such as foods, beverages, soups and sauces are used.
  • this soybean emulsified composition can be dried and used as a powdered soybean emulsified composition as a food raw material that substitutes whole milk powder for the food and beverage containing the soybean-derived material of the first invention.
  • the first invention includes such a powdery soybean emulsified composition and a soybean-derived raw material-containing food using the same.
  • This 1st invention is a soybean origin raw material containing food / beverage product which contains this soybean emulsion composition as a dairy product substitute. More specifically, the soy-derived raw material-containing food and drink of the first invention refers to a confectionery or bakery ingredient in which dairy products such as milk, fresh cream, and whole milk powder are used, confectionery, breads, desserts, beverages In soups, soups, sauces, etc., it means that part or all of the dairy product used is replaced with the present soybean emulsified composition.
  • an oil-in-water emulsion composition such as whipped cream used for one or more of cake, bread topping, coaching, sand, filling and kneading
  • water-in-oil emulsion compositions such as margarine and fat spread, flower pastes such as custard cream, water-containing chocolates such as ganache and raw chocolate, fluid, pasty and solid cheese-like foods.
  • Western confectionery and bread means general confectionery and bread such as short cakes and cheesecakes using the above-mentioned ingredients for pastry or bread.
  • Desserts include jelly, bavaroa, pudding, frozen confectionery, ice cream, etc. used for one or more of topping, coaching, sanding, filling, and kneading one or more of the above-mentioned ingredients for Western confectionery or bread means.
  • the beverages a part or all of the dairy products such as milk, fresh cream, whole milk powder, dairy beverages, fermented beverages, fruit juice beverages, coffee creams, etc. are replaced with the present soybean emulsified composition. Means something.
  • Soups and sauces include soups that use dairy products such as milk, fresh cream, whole milk powder, etc., in which all or part of the dairy product is replaced with the present soybean emulsified composition. Specifically, it means soup, stew, gratin, white sauce and so on.
  • This first invention is the above-mentioned flavor-improved soy-derived raw material-containing food or drink, that is, the present soybean for confectionery and bread ingredients, confectionery, breads, desserts, beverages, soups, sauces, etc.
  • Use of an emulsified composition as a dairy substitute is not particularly limited as long as its flavor characteristics are clearly shown, but is generally 0.5% by weight or more, preferably 1 to 30% by weight, most preferably 1-20% by weight. If it is less than the lower limit, the flavor characteristics of the present soybean emulsified composition are not clearly obtained, and therefore it is not preferable.
  • soybean emulsified composition at least one kind of sugar, starch, fats and oils, salt, emulsifier, emulsion stabilizer, thickener, seasoning, acidulant, spice, colorant, antioxidant, etc. It can be used together as appropriate.
  • sugars examples include maple syrup, honey, brown sugar, granulated sugar, maltose, trehalose, and maltose.
  • starches include raw starch, hydrolyzed starch, and modified starch.
  • the oil and fat is not particularly limited, but cocoa butter, rapeseed oil, soybean oil, sunflower oil, cottonseed oil, peanut oil, rice bran oil, corn oil, safflower oil, olive oil, sesame oil, palm oil, shea fat, monkey fat, Of vegetable oils and milk fats such as coconut oil and palm kernel oil, animal fats and oils such as beef tallow, pork fat and fish oil, single or mixed oils of the above fats, or processed fats and oils which have been cured, fractionated, transesterified, etc. One or more types can be exemplified.
  • emulsifier examples include lecithin, sucrose fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, diacetyltartaric acid monoglyceride and the like.
  • emulsion stabilizer and thickener examples include water-soluble soybean polysaccharide, pullulan, carboxymethylcellulose, agar, gelatin, pectin, carrageenan, guar gum, locust bean gum, sodium alginate and the like.
  • spices examples include turmeric, mustard and pepper.
  • coloring agent plant pigments such as carotene and lycopene and various synthetic pigments can be used.
  • antioxidants flavones such as tocopherols, catechins and rutin, ascorbic acid derivatives, plant extracts such as rosemary and bayberry extracts can be used.
  • this 1st invention improves the flavor of food-drinks containing soybean origin raw materials, such as confectionery, breads, desserts, beverages, soups, sauces, etc. which contain a soybean origin composition as a dairy product substitute.
  • soybean origin raw materials such as confectionery, breads, desserts, beverages, soups, sauces, etc. which contain a soybean origin composition as a dairy product substitute.
  • the method Significantly improve the flavor of pastry, bakery, desserts, beverages, soups, sauces, etc. by using this soy emulsified composition that has a significantly improved flavor compared to conventional soybean-derived ingredients It is possible to provide a food or drink excellent in flavor by substituting part or all with milk products such as milk, fresh cream and whole milk powder.
  • the flavor is greatly improved in the production of foods and beverages containing soy-derived ingredients such as confectionery, breads, desserts, beverages, soups, and sauces containing soy-derived compositions. It is the manufacturing method of the food-drinks containing the raw material derived from soybean. By replacing a part or all of dairy products such as milk, fresh cream and whole milk powder with this soy emulsified composition, it is easy to produce soy-derived raw-material-containing foods that are free from blueness and have richness derived from soy. can do.
  • the raw material of the acidic soybean material of the second invention is characterized by containing the present soybean emulsified composition.
  • the content of the present soybean emulsified composition in the raw material can be 30 to 100% by weight, preferably 60 to 100% by weight, more preferably 90 to 100% by weight.
  • the protein content per dry matter in the raw material is suitably 7% by weight or more, preferably 15% by weight or more, more preferably 20% by weight or more, and preferably 50% by weight or less.
  • the lipid content per dry matter in the raw material is suitably 10% by weight or more, preferably 20% by weight or more, more preferably 30% by weight or more, and preferably 75% by weight or less.
  • the lipid content per protein in the raw material is preferably 100% by weight or more, more preferably 120 to 250% by weight, and still more preferably 120 to 200% by weight.
  • the LCI value of soy protein contained in the raw material varies depending on the addition of the auxiliary raw material, but 55% or more is appropriate, preferably 58% or more, more preferably 60% or more, and further preferably 63%. Above, most preferably 65% or more.
  • the moisture content of the raw material affects the hardness of the resulting acidic soy material, and can be set as appropriate according to the desired quality, and can be 40 to 95% by weight for reference.
  • the raw material for the acidic soybean material of the second invention includes sugars, fats and oils, starches, proteins, pH adjusters, thickening polysaccharides, gelling agents, emulsifiers, fragrances, acidulants, and antioxidants.
  • Raw materials and additives such as agents and chelating agents can be added as appropriate.
  • assimilable saccharides may be added as a nutrient source for lactic acid bacteria as necessary.
  • glucose, fructose, sucrose, maltose, galactose, lactose, raffinose, trehalose, soybean oligosaccharide, fructooligosaccharide, xylooligosaccharide and the like can be used.
  • sugar raw materials may be used alone or in combination of two or more.
  • the amount of the assimilating saccharide is suitably 0 to 50% by weight, preferably 0 to 20% by weight, based on the solid content of the soybean emulsified composition.
  • the amount of lipid in the acidic soybean material of the second invention is the amount of lipid derived from the present soybean emulsified composition and sufficiently rich.
  • fats and oils can be added separately in order to increase the fat by further increasing the fat, or to adjust the hardness of the body of the acidic soybean material.
  • the lipid content in the acidic soybean material can be adjusted to 3 to 40% by weight.
  • As fats and oils to be added one or two or more kinds selected from processed fats and oils such as animal and vegetable fats and oils, hardened oils, fractionated oils, transesterified oils, diglycerides, and medium chain fatty acid-containing fats and oils can be used.
  • animal and vegetable oils examples include soybean oil, rapeseed oil, rice oil, sunflower oil, safflower oil, palm oil, palm kernel oil, palm oil, corn oil, cottonseed oil, peanut oil, monkey fat, shea fat, beef fat, milk fat, Examples include pork fat, cacao butter, fish oil, whale oil, mustard oil, etc., but it is preferable to use vegetable oil.
  • vegetable oil examples include pork fat, cacao butter, fish oil, whale oil, mustard oil, etc.
  • the melting point of fats and oils can be appropriately selected in consideration of the hardness of the product, but is preferably 5 to 40 ° C.
  • the raw material of the acidic soybean material of the second invention can be homogenized by an appropriate homogenizing means such as a homogenizer, if necessary, such as when fats and oils are separately added.
  • a homogenizer such as a homogenizer
  • the homogenization pressure at that time affects the hardness of the structure of the cream cheese-like food, so that it can be appropriately set according to the quality desired by those skilled in the art, but generally 2.5 to 15 MPa (25 to 150 kg / cm 2). 2 ) is appropriate.
  • the said raw material is acidified. That is, the pH of the raw material is adjusted to be acidic.
  • an acidifying means a method of adding an acid, a method of fermenting lactic acid bacteria, or a method of combining them can be used. In general, a pH of 3.5 to 6.0, preferably 4 to 5.9, more preferably 5.0 to 5.8 is appropriate. What is necessary is just to adjust to the same pH range also in the case of acid addition. If the pH is too low, the acidity becomes strong and roughening tends to occur. Moreover, when pH is too high, the sour feeling by acidification will weaken, especially in the case of lactic acid fermentation, fermentation flavor will become scarce.
  • the acid to be used can be used without any limitation, and inorganic acids such as phosphoric acid, hydrochloric acid and sulfuric acid, and organic acids such as citric acid, malic acid, lactic acid, gluconic acid and GDL An acid can be illustrated and these can be used individually by 1 type or in mixture of 2 or more types, but it is preferable to use an organic acid on flavor.
  • inorganic acids such as phosphoric acid, hydrochloric acid and sulfuric acid
  • organic acids such as citric acid, malic acid, lactic acid, gluconic acid and GDL
  • An acid can be illustrated and these can be used individually by 1 type or in mixture of 2 or more types, but it is preferable to use an organic acid on flavor.
  • lactic acid bacteria can be those used for fermented dairy products such as ordinary yogurt, lactic acid bacteria beverages and cheese, and are not particularly limited.
  • Lactobacillus casei Lactobacillus plantarum, Lactobacillus helveticus, Lactobacillus bulgaricus, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus lactis, Lactobacillus salivalus salivaius, Lactobacillus gallinarum , Lactobacillus amylovorus, Lactobacillus brevis brevis, Lactobacillus fermentum, Lactobacillus mari, Lactobacillus delbrukki, Lactobacillus johnsonii, Lactobacillus sanfrancisensis, Lactobacillus panex, Lactobacillus Comoensis, Lactobacillus italicas, Lactobacillus raikimani, Lactobacillus
  • the genus Bifidobacterium is also included in the lactic acid bacterium, such as Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bifidobacterium breve, Bifidobacterium adrecentis, Bifidobacterium angularum, Bifidobacterium catenatum, Bifidobacterium pseudocatenatum, Bifidobacterium denthium, Bifidobacterium globosm, Bifidobacterium Phytobacterium pseudolongum, Bifidobacterium cuniculi, Bifidobacterium coelinum, Bifidobacterium animalis, Bifidobacterium thermophilum, Bifidobacterium boum, Bif D.
  • Bifidobacterium bifidum Bifidobacterium longum, Bifidobacterium
  • Bifidobacterium asteroides Bifidobacterium indicum, Bifidobacterium gallicum, Bifidobacterium lactis, Bifidobacterium inopinatum, Bifidobacterium denticorens, Bifidobacterium Fidobacterium prolam, Bifidobacterium swiss, Bifidobacterium gallinarum, Bifidobacterium luminantium, Bifidobacterium merlicum, Bifidobacterium circulare, Bifidobacterium minimum, Bifidobacterium Examples include Fidobacterium sabutyl and Bifidobacterium coryneform. Moreover, these lactic acid bacteria can be used individually or in arbitrary combinations of 2 or more types.
  • the addition amount of lactic acid bacteria can be adjusted according to fermentation temperature and fermentation time.
  • the fermentation conditions are 10 to 50 ° C. for 3 to 48 hours, preferably 15 to 45 ° C. for 4 to 30 hours.
  • the pH after fermentation can be fine-tuned appropriately using alkali or acid according to taste.
  • Acidified products adjusted to an appropriate pH can be subjected to a step of separating the whey by centrifugation or the like and collecting the card if necessary.
  • a whey-separated cheese-like acidic soybean material having a high solid content and a solid or semi-solid hardness can be produced, and can be adjusted to a less sour taste by separating whey.
  • the solid content is low, and a liquid or pasty whey non-separable cheese-like acidic soybean material can be produced. Whether or not to separate whey can be appropriately determined in consideration of the physical properties suitable for the use of the food produced by those skilled in the art.
  • the acidified product before separating the whey, or a card from which the whey has been removed from the acidified product chloride such as sodium chloride and potassium chloride as necessary, and polyphosphoric acid Salts such as phosphates such as sodium can be added.
  • chloride such as sodium chloride and potassium chloride
  • polyphosphoric acid Salts such as phosphates such as sodium
  • phosphates such as sodium
  • the amount of phosphate added is not limited, but is preferably 0.5 to 15% by weight per protein in the acidic soybean material. If the amount of phosphate is too small, the effect of reducing the roughness is reduced, and if it is too much, the astringent taste tends to be strong.
  • the acidic soybean material of the second invention of the present invention can be made into a cheese type material or a yogurt type material by changing the type of raw material, the type of lactic acid bacteria and the fermentation conditions (temperature, time, etc.).
  • a cheese-type material refers to a material that is obtained by the same production method as that for existing cheeses or that has the same quality (hardness and flavor) as existing cheeses by adding auxiliary materials such as starch and fats and oils.
  • Existing cheese types can be broadly divided into natural cheese and processed cheese.
  • Typical examples of the second invention include non-aged type fresh cheese and cream cheese, and specifically as fresh cheese, cottage cheese, fromage blanc, mozzarella cheese, ricotta cheese, mascarpone, curse martu, There are feta cheese, paneer, luchamp.
  • an aged cheese type material such as wash cheese, white mold cheese, blue mold cheese, semi-hard cheese, and hard cheese.
  • aged cheese include Gouda cheese, Edam cheese, Camembert cheese, and cheddar cheese.
  • a yoghurt type material it is obtained by the same manufacturing method as that of an existing yoghurt, or a material having the same quality (physical properties and flavor) as an existing yoghurt by adding an auxiliary material.
  • Existing yogurt types include yogurts made in countries around the world, such as Bulgarian yogurt in Japan and Kefir yogurt in Russia.
  • the acidic soybean material of the second invention has a rich flavor similar to that of fermented dairy products in combination with a good rich taste of soy and a rich taste due to acidification (particularly lactic acid fermentation), and has a sharp aftertaste. Is also good and the physical properties are smooth, so that it can be used partially or entirely for various food applications similar to fermented milk products such as cheese and yogurt (fermented milk product substitute). For various foods, it can be used as a main raw material as a base for blending, or as a secondary raw material for kneading, filling, topping or nappe.
  • the main raw material is a raw material used as a basis for blending foods, but is not limited, but generally refers to those blended in foods by 20% by weight or more.
  • the auxiliary raw material is a raw material to be mixed, injected, adhered or applied to the main raw material serving as a base for blending.
  • this acidic soybean material can be provided as it is as a product for eating.
  • processed food materials such as a flour paste, a spread, a filling, and a sour cream
  • sauces such as cream sauce and curry sauce
  • it can be used as a raw material for sauces such as cream sauce and curry sauce, or can be directly used as a raw material for confectionery and bread. It can also be used for filling and topping fishery products such as chikuwa and meat products such as hamburgers.
  • both confectionery and Japanese confectionery have good flavor compatibility, and among confectionery and Japanese confectionery, fresh confectionery is preferred.
  • Western confectionery includes cheesecake, tarts, sponge cakes, pound cakes, castella, baumkuchen, cream puffs, waffles, cookies, pie, souffle, tiramisu, pudding, bavaria, and Japanese confectionery. , Yokan, Uiro, Dorayaki, Taiyaki, etc.
  • an example of a Western confectionery will be shown as a more specific embodiment of the confectionery.
  • Rare cheesecake-like food Use whey non-separable acidic soy material as a main ingredient instead of filled-type cheese material (for example, “Fromage Blanc Assortier” manufactured by Fuji Oil Co., Ltd.) Can be created.
  • the acidic soybean material is preferably blended in the food in an amount of 5 to 70% by weight, preferably 20 to 60% by weight.
  • those usually used for rare cheesecakes can be used.
  • milk raw materials such as milk and cream
  • sugars such as liquid sugar and granulated sugar
  • gels such as egg white and gelatin used for meringue An agent or the like can be used.
  • soy milk, soybean powder, separated soybean protein, soybean cream prepared from these soybean ingredients, or the like can be used instead of the milk ingredients.
  • This rare cheesecake-like food has a quality comparable to that of a rare cheesecake made of cheese or filled type cheese, has a rich flavor, and has a smooth texture with a refreshing feel derived from soybeans.
  • Baked cake-like foods Use whey-separated acidic soy materials as the main ingredients instead of filled-type cheese materials (for example, “Crime Fromage” manufactured by Fuji Oil Co., Ltd.) to create baked cheesecake-like foods be able to.
  • the acidic soybean material is preferably blended in the food in an amount of 5 to 70% by weight, preferably 20 to 60% by weight.
  • those commonly used for baked cheesecake can be used, but for example, milk ingredients such as cream, sugars such as granulated sugar, white chocolate, eggs such as whole eggs and egg yolks, corn starch, etc. Can be blended with cheese dough.
  • This baked cheesecake-like food has a rich flavor that is not inferior in appearance to that produced with cheese or filled-type cheese.
  • the texture is given a new texture such as a crumpled rice cake, and a new cake different from ordinary baked cheese can be obtained.
  • the egg yolk substitute composition of the third invention is characterized by containing the present soybean emulsified composition, and can substitute a part or all of the egg yolk blended in each food.
  • Egg yolks that can be substituted for the egg yolk in each food by the present third invention are raw egg yolk, sterilized egg yolk, salted egg yolk, sweetened egg yolk, dried egg yolk, and the like.
  • the egg yolk substitute composition of the third invention is used as an egg yolk substitute composition improved in quality and physical properties by adding sugar, starch, spices, antioxidants, oils and fats, and coagulants to the soybean emulsified composition. can do. One or more of these substances can be used in combination.
  • the egg yolk substitute composition of the third aspect of the invention is a group of sugars, starches, spices, antioxidants, oils and fats and coagulants in which the soy emulsion composition is allowed to act on a protein cross-linking enzyme. It can be used as an egg yolk substitute composition with improved quality and physical properties by allowing a protein cross-linking enzyme to act on one added with at least one selected from
  • the egg yolk substitute composition of the third invention of the present invention can be made into an egg yolk substitute composition in which a sugar is added to the soybean emulsified composition to further improve the flavor.
  • saccharides are oligosaccharides and oligosaccharides, specifically, maple syrup, honey, brown sugar, granulated sugar, sugar, caramel, fructose, fructose glucose liquid sugar, starch syrup, maltose, maltotriose, trehalose, A multitose etc. can be illustrated.
  • One or more of these saccharides can be used in combination.
  • the content of the saccharide is preferably 0.5 to 50% by weight, more preferably 3 to 30% by weight in the egg yolk substitute composition.
  • the egg yolk substitute composition of the third invention can be made into an egg yolk substitute composition in which a spice is added to the soybean emulsified composition to further improve the flavor.
  • a spice is added to the soybean emulsified composition to further improve the flavor.
  • a spice is added to the soybean emulsified composition to further improve the flavor.
  • spices mustard, wasabi, thyme, basil, anise seed, clove, fennel, ginger, cinnamon, pepper, turmeric, paprika, fenugreek seed, nutmeg, star anise, oregano, laurel, marjoram, rosemary, peppermint, tarragon, Examples include dill seeds.
  • One or more of these spices can be used in combination.
  • the content of the spice is preferably 0.001 to 20% by weight, more preferably 0.01 to 10% by weight in the egg yolk substitute composition.
  • the egg yolk substitute composition of the third invention of the present invention can be made into an egg yolk substitute composition in which an antioxidant is added to the soybean emulsified composition and flavor deterioration due to oxidation is suppressed.
  • an antioxidant examples include tocopherol, bayberry extract, ascorbic acid, catechins, tea extract, a substance having a chelating effect such as sodium citrate, and the like.
  • One or more of these antioxidants can be used in combination.
  • the content of the antioxidant is preferably 0.001 to 20% by weight, more preferably 0.01 to 10% by weight in the egg yolk substitute composition.
  • the egg yolk substitute composition of the third invention can be an egg yolk substitute composition obtained by adding fats and oils to the soybean emulsified composition and imparting a physical property improving function such as imparting a body feeling.
  • the oil and fat may be any oil and fat suitable for edible use.
  • These fats and oils can use 1 or more types together.
  • the content of the oil or fat is preferably 0.5 to 50% by weight, more preferably 3 to 30% by weight in the egg yolk substitute composition.
  • the egg yolk substitute composition of the third invention of the present invention can be an egg yolk substitute composition obtained by adding starch to the present soybean emulsified composition and imparting a physical property improving function such as a body feeling.
  • starch corn starch, waxy corn starch, wheat starch, rice starch, potato starch, tapioca starch, sweet potato starch, sago starch and the like can be used, and these modified starches can also be used.
  • modified starch for example, oxidized starch, acid-treated starch, enzyme-treated starch, starch acetate, phosphate starch, succinate starch, octenyl succinate starch, hydroxypropyl starch, cross-linked starch, wet heat-treated starch, etc. can be used. it can. One or more of these starches can be used in combination.
  • the content of the starch is preferably 0.5 to 50% by weight, more preferably 3 to 30% by weight in the egg yolk substitute composition.
  • the egg yolk substitute composition of the third invention of the present invention can be an egg yolk substitute composition obtained by adding a coagulant to the soybean emulsified composition to give an egg yolk-like coagulation function.
  • a coagulant include bittern, calcium chloride, magnesium chloride, glucono delta lactone, calcium sulfate and the like.
  • One or more of these coagulants can be used in combination.
  • the content of the coagulant is preferably 0.001 to 10% by weight, more preferably 0.01 to 5% by weight in the egg yolk substitute composition.
  • the egg yolk substitute composition of the third invention of the present invention can be made into an egg yolk substitute composition having improved emulsifying performance such as emulsifying power by acting a protein cross-linking enzyme on the soybean emulsified composition. This high emulsification performance is exerted particularly when preparing an acidic emulsion.
  • the protein cross-linking enzyme is not particularly limited as long as it is an enzyme that catalyzes cross-linking of protein molecules. For example, a condensation reaction of a glutamine residue and a lysine residue involving amino groups, a condensation reaction of an asparagine residue and a lysine residue, etc. And those that catalyze reactions involving the ⁇ -amino group.
  • transglutaminase that catalyzes the condensation of glutamine and lysine residues
  • thiol / disulfide exchange enzyme that condenses thiol groups and catalyzes the formation of disulfide bonds.
  • transglutaminase is preferably used.
  • the origin of transglutaminase is not particularly limited, and any of those derived from animals, microorganisms, and plants can be used.
  • a purified enzyme may be used, or a commercially available preparation may be used.
  • the amount of the protein cross-linking enzyme used is preferably 0.1% by weight or more, more preferably 0.5% by weight or more, and even more preferably 0.9% by weight or more based on the solid content of the present soybean emulsion composition. Moreover, since there may be no difference in the effect even if the amount of enzyme is too large, it is preferably 15% by weight or less, more preferably 10% by weight or less.
  • the working temperature of the protein cross-linking enzyme is generally 10 to 80 ° C, preferably 20 to 70 ° C, more preferably 40 to 60 ° C. If the temperature is too low, the enzyme reaction may be slow, and if the temperature is too high, inactivation of the enzyme may be promoted.
  • the action time of the protein cross-linking enzyme is generally 0.01 to 120 minutes, preferably 1 to 60 minutes. If the action time is too short, a sufficient reaction effect may not be obtained, and even if the action time is too long, there is no difference in effect.
  • the egg yolk substitute composition of the third invention is added with an emulsifier, a thickener, a gelling agent, a protein, an inorganic salt, an organic salt, a colorant, a fragrance, an acidulant and the like as necessary. Can be used.
  • the egg yolk substitute composition containing the present soybean emulsified composition can be used in various foods as described below. By blending an egg yolk substitute composition containing the present soybean emulsified composition, a flavorful egg yolk substitute food that is rich and has a suppressed blue odor derived from soybeans can be obtained.
  • the use amount of the egg yolk substitute composition containing the soybean emulsified composition is not particularly limited as long as the flavor characteristic of the soybean emulsion composition is clearly shown, but is generally 0.5 wt. % Or more, preferably 0.5 to 60% by weight, most preferably 1 to 50% by weight. If it is less than the lower limit, the flavor characteristics of the present soybean emulsified composition are not clearly obtained, and therefore it is not preferable.
  • the egg yolk substitute food of the third invention can be produced by using a general production method used for producing each food.
  • the egg yolk substitute food of the third invention includes the above-mentioned egg yolk substitute composition, sugars, starches, spices, antioxidants, fats and oils, coagulants, emulsifiers, thickeners, gelling agents, proteins, inorganic salts It can be produced by adding organic salts, coloring agents, fragrances, acidulants, etc., and can be produced by allowing a protein cross-linking enzyme to act on an egg yolk substitute composition.
  • Examples of the egg yolk substitute food of the third invention include seasonings, confectionery, sauces, sugar beet, and the like.
  • seasoning in the present invention examples include dressings, sauces, mayonnaise, and dry seasonings.
  • dressings include semi-solid dressings, emulsified liquid dressings, and separated liquid dressings.
  • Sauces are seasoning liquids used for boiling, and examples thereof include grilled meat sauce, grilled chicken sauce, teriyaki sauce, shabu-shabu sauce, meat, and seafood sauce.
  • mayonnaise examples include mayonnaise, mayonnaise-like seasonings, mayonnaise-like seasonings, and the like.
  • Mayonnaise with good flavor can be prepared.
  • confectionery in the present invention examples include creams, desserts, baked confectionery, and the like.
  • Creams are those obtained by mixing and emulsifying an oil phase and an aqueous phase, and examples thereof include custard cream, ice cream, and whipped cream.
  • custard cream custard cream-like food can be prepared by using the egg yolk substitute composition of the third invention and white chocolate in combination.
  • the white chocolate used in the third invention includes white chocolate for coating.
  • the chocolate in this invention means the chocolate prescribed
  • the blending ratio of the white chocolate of the third invention and the egg yolk substitute composition is not particularly limited, and is generally a ratio of 1:99 to 99: 1 (weight ratio), and is appropriately weighted according to physical properties such as the melting point of white chocolate. It can be prepared by changing the ratio.
  • desserts include pudding, bavaroa and brulee.
  • pudding include custard type pudding.
  • the custard type pudding includes a grill type that is steamed in an oven and a steam type that is steam heated in a retort kettle or steamer.
  • Examples of baked goods include sponge cake, pound cake, chiffon cake, pancake, roll cake, castella, donut, madeleine, biscuit, cookie, brioche, muffin, waffle, brownie, souffle and the like.
  • Sauces examples of the sauces in the present invention include carbonara sauce, cream sauce, cream soup, hollandaise sauce, custard sauce, tartar sauce and the like, and soup is also included as exemplified above.
  • the soybean processed food of the fourth invention is characterized in that the soybean emulsion composition is used as a raw material.
  • the soybean emulsion composition is used as a raw material.
  • it is a processed soybean food processed using soybean raw materials such as soy milk and powdered soybean protein, wherein a part or all of the soybean raw material is replaced with the present soybean emulsified composition. More specifically, it is tofu, yuba, soybean seasoning, deep-fried tofu, cancer throbbing, deep-fried deep-fried, soy milk for drinking, and the like categories.
  • the present soybean emulsified composition to be blended with processed soybean foods can be blended by replacing all or part of soybean raw materials such as soy milk and powdered soy protein which are ordinary raw materials. Thereby, the texture and flavor of the processed soybean food can be improved.
  • the substitution rate depends on the type of processed soybean products, it can be 10% by weight or more of soybean raw materials such as soy milk, preferably 15% by weight or more, more preferably 20% by weight or more, and 25% by weight or more. You can also. Further, depending on the desired quality, it may be 50% by weight, 70% by weight, 90% by weight or 100% by weight.
  • the aspect of a specific processed soybean food is demonstrated, the kind of processed soybean food is not limited to the following aspect.
  • Tofu Tofu can be produced by adding a coagulant to the present soybean emulsified composition or a mixture of the present soybean emulsified composition and soy milk and coagulating the protein by heating.
  • Tofu can be produced by a known production method and can be produced without any particular difficulty.
  • As the coagulant bittern (magnesium chloride), calcium chloride, calcium sulfate, GDL and the like can be used.
  • the amount of the coagulant added is not particularly limited, but may be, for example, 0.1 to 5% by weight, preferably 0.3 to 3% by weight, based on the present soybean emulsified composition and soy milk dry matter.
  • the tofu hardness can be adjusted as appropriate by increasing or decreasing the amount of coagulant.
  • the coagulant may be added in a small amount.
  • the tofu obtained is very creamy compared to conventional tofu, has a mouthfeel that melts in the mouth, and has a blue-smelling soybean odor and a rich flavor.
  • the various additives currently added in general manufacture can be added to tofu. For example, in addition to the coagulant, salts, oligosaccharides, polysaccharides, starches, proteins and the like can be added alone or in combination.
  • Yuba This soybean emulsified composition is used alone or in combination with soy milk as a raw material, and this is heated or frozen to produce a membrane-like protein denatured product. Yuba can be manufactured.
  • the manufacturing method of yuba can be performed by a known means.
  • the resulting yuba has a rich texture like freshly cooked yuba (pumped yuba) and has a richer flavor than normal yuba.
  • salts, polysaccharides, starches, proteins and the like can be added to the present soybean emulsified composition alone or in combination to prepare yuba.
  • Soy seasoning The soybean emulsified composition can be blended with the raw material of the seasoning to produce a soy seasoning.
  • seasoning examples include ponzu, soy sauce, mayonnaise, dressing, sauce, ketchup, sauce, dashi, etc., and liquid or powder seasonings attached to instant noodles.
  • the soybean emulsified composition can be blended in the seasoning, for example, 20 to 80% by weight.
  • Deep-fried / Cancered / Deep-fried Soy emulsified composition can be used as a part of the raw material, and processed soybean foods such as deep-fried, deep-fried or deep-fried can be prepared by a known production method. These processed soybean foods may use tofu as a raw material dough by a traditional manufacturing method, or a soybean protein paste obtained by kneading and emulsifying powdered soy protein, water and fat with a cutter etc. as a raw material dough Also good. In the case of deep-fried food, if the present soybean emulsified composition is used, the same texture and flavor can be imparted to tofu as when it is used.
  • this soy emulsion composition when used for deep-fried chicken or cancer, it not only gives a rich flavor of soybeans, but also promotes the expansion of the dough raw material when fried and improves the expansion rate The effect to make can be given. And the expansion rate improves, The liquid absorptivity of the seasoning liquid after cooking can also improve, and a more juicy food texture can be given.
  • Drinking soymilk is not a soymilk for the production of tofu products, but a beverage that uses soymilk that has been widely consumed by general consumers in recent years. Includes all beverages used. If this soybean emulsified composition is used for drinking soymilk, the richness and creamy feeling of soybeans can be imparted.
  • the processed processed food of the fourth invention has the same texture after freezing and thawing as before by freezing by adding known anti-freezing agents such as oligosaccharides, polysaccharides, starches and gelatin. It can also be set as the processed soybean food provided with freeze resistance. These can be commercialized as frozen foods such as frozen tofu, frozen yuba, deep-fried deep-fried, frozen cancer, and deep-fried frozen oil.
  • the addition amount of the freezing denaturation inhibitor is preferably 0.1 to 10% by weight in the tofu.
  • the conventional frozen tofu has a light and light soybean flavor, and the texture is hard and easy to taste. The effect similar to the use effect to the kind is obtained.
  • Processed dried soybean food The processed soybean food of the fourth invention is finally dried tofu, dried yuba, dried soybean by various means such as freeze drying, spray drying, microwave drying, oven drying, natural drying, and vacuum drying. Dried soybean processed products such as seasonings, fried dried oil, and powdered soy milk can be obtained.
  • the processed soybean food of the fourth aspect of the present invention is fried, the fried fried food is seasoned, for example, by immersing it in a seasoning liquid containing various seasonings, and can be used as an instant ingredient.
  • the deep-fried fried food of the fourth invention has a good swelling due to the use of the present soybean emulsified composition, so that the volume and excellent restorability can be maintained even when seasoning and maintaining the volume after drying and pouring hot water to restore it. (Removable hot water).
  • the present soybean emulsified composition has a remarkable effect of reducing serum cholesterol and improving renal function.
  • the serum cholesterol-reducing action and renal function-improving action can be seen by eating an appropriate amount of the soybean emulsified composition. That is, the present soybean emulsified composition can be used as an action body of a serum cholesterol reducing composition or a renal function improving composition.
  • the content of the soybean emulsified composition in the serum cholesterol reducing composition and the renal function improving composition of the fifth invention is 1 to 100% by weight, preferably 50 to 100% by weight, and more preferably 80%. ⁇ 100% by weight, most desirably 100% by weight.
  • the “food that exhibits a serum cholesterol-reducing action” and the “food that exhibits a renal function-improving action” can be easily prepared in the composition for reducing serum cholesterol of the fifth invention with good flavor and serum cholesterol. It is derived for the first time by having a reduction effect, and is achieved for the first time by the fifth invention.
  • a food for specified health use or a special-purpose food that exhibits an action for reducing serum cholesterol or an action for improving kidney function.
  • This method for producing a food exhibiting an action for reducing serum cholesterol or an action for improving renal function is technically characterized by containing the “composition for reducing serum cholesterol” or the “composition for improving kidney function” of the fifth invention. It is what has.
  • the soy emulsion composition is a milk substitute composition and egg yolk substitute composition, a milk substitute composition and a serum cholesterol or kidney function improving composition, an egg yolk substitute composition and serum cholesterol or kidney function. It can function as a composition for improvement.
  • % and parts mean “% by weight” and “parts by weight” unless otherwise specified.
  • Analysis of lipids was performed according to the chloroform / methanol mixed solvent extraction method unless otherwise specified.
  • soybean emulsified composition 2 The soybean emulsified composition A prepared in Production Example 1 was added 0.5 times by weight and further homogenized with a high-pressure homogenizer at 13 MPa, and then the homogenized liquid was heated at 142 ° C. for 7 seconds by a steam direct blowing method. The insoluble fiber was separated and removed at 6,000 ⁇ g with a continuous centrifuge, and a supernatant fraction was obtained.
  • soybean emulsified compositions A and B obtained in Production Examples 1 and 2 were partially lyophilized for analysis, dried as general components, protein per dry matter (by Kjeldahl method), lipid (chloroform / methanol mixed solvent) (By an extraction method) and ash content were measured, and further, LCI value was analyzed as an estimated value of lipoxygenase protein content and LP content by SDS-PAGE.
  • soy flour used as a raw material and a commercially available soybean emulsified composition “Soy Supreme Kreme” (SunOpta Grains and Foods Group, presumed to be produced by the method of US Pat. No. 6,548,102) ) And powder type) were similarly analyzed. Each analysis value is shown in Table 2.
  • Soy emulsified compositions A and B were rich in lipid content relative to protein compared to soybean flour, and the protein composition was significantly different from soybean flour and commercially available soybean emulsified compositions. That is, since the LCI value was as high as 60 or more, LP was concentrated, and the lipoxygenase protein content was also high.
  • the soybean emulsified composition having such a composition is a novel component composition not found in conventional soymilk or okara.
  • the flavors of these compositions A and B are also concentrated on the natural taste inherent in existing soymilk and soybeans, and unpleasant tastes such as the blue odor, astringency, and astringency of soybeans, which were previously considered problems. No, it had a very rich flavor.
  • the commercially available soybean emulsified composition had a lower LCI value than soybean flour and a lower lipoxygenase protein than the compositions A and B, and was less flavorful and a strong flavor of green grass and astringency.
  • Example a1 Preparation of whipped cream 29.9% soybean emulsified composition B prepared in Production Example 2, 29.9% hydrogenated palm kernel oil (melting point 34 ° C.), fructose glucose sugar (FC; Oji Cornstarch Co., Ltd.) 1.4%, Oligotose (Mitsubishi Chemical Foods) 1.4%, Water 37.0%, Lecithin 0.2%, Sucrose fatty acid ester “Ryoto Sugar Ester S-570” (Mitsubishi Chemical Foods) Co., Ltd.) 0.1%, sucrose fatty acid ester “Ryoto Sugar Ester S-1670” (manufactured by Mitsubishi Chemical Foods Co., Ltd.) 0.1%, sodium polyphosphate 0.015%, 60 ° C., 15 Pre-emulsified for minutes.
  • soybean-containing whipped cream stock solution To 1 kg of the cream stock solution, 70 g of granulated sugar was added and whipped for 2 minutes 30 seconds by high-speed stirring with a Hobart mixer. The overrun of the obtained soybean-containing whipped cream was 159.4%. This soy-containing whipped cream melted in the mouth with a cool sensation, had no blue odor characteristic of soybeans, and had a high overall evaluation with a rich taste.
  • Example a1 The soybean emulsified composition B of Example a1 was dispersed in water with a commercially available soybean emulsified composition “Soy Supreme Kreme” (manufactured by SunOpta Grains and Foods Group, powder type).
  • a soy-containing whipped cream stock solution was prepared in the same manner as in Example 1 by substituting the dispersion liquid adjusted to 18.2% equivalent to the product B.
  • 70 g of granulated sugar was added and whipped for 2 minutes and 50 seconds by high-speed stirring with a Hobart mixer.
  • the overrun of the obtained soybean-containing whipped cream was 155.0%.
  • the soy-containing whipped cream melted in the mouth with a cool sensation, but the soy-specific blue odor was slightly strong and the body was weak and the overall evaluation was low.
  • Table 3 shows the flavor evaluation results of Example a1, Comparative Example a1, and Reference Example a1.
  • the flavor evaluation shown in each following example is sensory evaluation by 10 panelists, and is panel average evaluation in the following evaluation criteria.
  • evaluation criteria -: Not felt at all ⁇ : Not felt at all +: Feeling ++: Feeling slightly strong ++++: Feeling strong ⁇ : Strong and no blue odor ⁇ : Rich and almost no blue odor ⁇ : Low in strength and blue There is a smell ⁇ : There is no richness, strong blue odor
  • Example a2 Preparation of ice cream 50% of the soybean emulsified composition B prepared in Production Example 2, 11% of super white sugar, and 8% of honey (sakura pure honey) were mixed, stirred, and then stirred at 70 ° C using an ice creamer. The mixture was stirred and cooled to obtain a frozen ice cream stock solution. To 69 parts of this stock solution, 31 parts of the soybean whipped cream obtained in Example a1 were added and mixed to obtain a soy ice cream. When this soy ice cream was stored at ⁇ 20 ° C. for 15 days and evaluated for flavor, it was found to have a cool and light texture, no blue odor peculiar to soybeans, and a high overall evaluation with a rich taste.
  • Example a3 50% soy emulsified composition B prepared in Production Example 2, 11% super white sugar, 7% brown sugar, 1% water were mixed, stirred, and then stirred and cooled from 70 ° C. using an ice creamer, and then frozen. A cream stock solution was obtained. To 69 parts of this stock solution, 31 parts of the soybean whipped cream obtained in Example a1 were added and mixed to obtain a soy ice cream. When this soy ice cream was stored at ⁇ 20 ° C. for 15 days and evaluated for flavor, it was found to have a cool and light texture, no blue odor peculiar to soybeans, and a high overall evaluation with a rich taste.
  • Example a2 The soybean emulsified composition B of Example a2 was dispersed in water with a commercially available soybean emulsified composition “Soy Supreme Kreme” (manufactured by SunOpta Grains and Foods Group). The dispersion was adjusted to 18.2%, which was equivalent to product B, and a frozen ice cream stock solution was obtained in the same manner as in Example a2. Soybean ice cream was obtained by adding and mixing 31 parts of the soy whipped cream obtained in Comparative Example a1 to 69 parts of this stock solution. The flavor of this soy ice cream was evaluated after storage at ⁇ 20 ° C. for 15 days. The taste was cool and light, but the soy-specific blue odor was slightly strong and the rich taste was weak. It was low.
  • Example a2 The soy emulsion composition B of Example a2 was replaced with the fresh cream of Reference Example a1 to obtain a frozen ice cream stock solution as in Example a2. Ice cream was obtained by adding and mixing 31 parts of the whipped cream obtained in Reference Example a1 to 69 parts of the stock solution. When this ice cream was evaluated for flavor after storage at ⁇ 20 ° C. for 15 days, it had a cool, light texture and excellent milkiness and richness, and was highly evaluated.
  • Table 4 shows the flavor evaluation results of Examples a2 to a3, Comparative Example a2, and Reference Example a2.
  • Example a4 Preparation of cream soup Sweet corn 30.1%, roasted onion (manufactured by Ebara Co., Ltd.) 6.0%, soybean emulsified composition A prepared in Production Example 1 15.0%, upper white sugar 3.0 %, Salt 0.3%, consomme chicken flavor powder 0.5%, baking soda 0.1%, water 45.1%, white pepper Was prepared.
  • This soup had a thick and smooth texture, no blue odor characteristic of soybeans, and a high overall evaluation with a rich taste.
  • Example a3 Soy emulsified composition A of Example a4 was replaced with non-adjusted soymilk (solid content 6.5%, manufactured by Fuji Oil Co., Ltd.) to prepare a corn cream soup tailored with soymilk in the same manner as Example a4.
  • This soup had a thick and smooth texture and almost no soy-like blue odor, but had a weak body.
  • Example a3 A corn cream soup was prepared in the same manner as in Example a4, except that the soybean emulsified composition A in Example a4 was replaced with the fresh cream in Reference Example a1. This soup had a thick and smooth texture and a high overall evaluation with milky and rich taste.
  • Table 5 shows the flavor evaluation results of Example a4, Comparative Example a3, and Reference Example a3.
  • Example a5 Milk pudding-like raw material-containing food and beverage containing soybeans 90.81% of the soybean emulsion B prepared in Production Example 2, 9.08% sugar, bittern for tofu (41 Brix) 0.11% Were added and mixed, and then filled into a cup container and steamed at 95 ° C. for 25 minutes to obtain a milk pudding-like raw material containing soybean-derived material.
  • This soy-derived raw material-containing food and drink had a slightly supple and smooth pudding-like texture, no blue odor peculiar to soybeans, and a high overall evaluation with a rich taste.
  • Example a6 Preparation of fruit milk-like soybean beverage 9.0% of sugar was dissolved in 68.3% of water, and 17.5% of the soybean emulsion B prepared in Production Example 2 was stirred while stirring the solution. Concentrated banana juice 5.0% was added and mixed. Subsequently, 50% citric acid aqueous solution was added to adjust the pH to 6.0. Next, the mixture was heated to 70 ° C., homogenized at a high pressure at 15 MPa, heated to 93 ° C., 0.2% of banana flavor was added, and the mixture was cooled to 20 ° C. in cold water. The obtained banana flavored drink had no blue smell peculiar to soybeans, and had a high overall evaluation with a rich taste.
  • Example a7 Preparation of a fruit milk-like soy beverage
  • a mixture of 9.0% sugar and water-soluble soybean polysaccharide “Soya Five S-ZR100” (manufactured by Fuji Oil Co., Ltd.) was added to 78.4% water. The mixture was stirred and dissolved at 10 ° C. for 10 minutes. This aqueous solution was cooled to 20 ° C., and 10.2% soybean emulsion B prepared in Production Example 2 and 2.0% 5-fold concentrated white peach clear fruit juice were added and mixed. Subsequently, 50% citric acid aqueous solution was added to adjust the pH to 3.8.
  • the obtained peach flavored beverage had no blue smell peculiar to soybeans and had a high overall evaluation with a rich taste.
  • Example a8 Preparation of cheese souffle-like soy milk souffle (1)
  • 200 g of egg yolk was added, 30 g of granulated sugar was added, and lightly whipped with medium stirring. Thereafter, 300 g of soybean emulsion B and 80 g of weak flour were sequentially added while stirring at low speed.
  • 100 g of granulated sugar and 70 g of trehalose (manufactured by Hayashibara Shoji Co., Ltd.) were added to 220 g of egg white, and a meringue foamed with stirring was added and mixed to prepare a soymilk souffle dough having a specific gravity of 0.29.
  • Example a9 Preparation of cheese souffle-like soymilk souffle (2) 280 g of soybean emulsion B was placed in a Hobart mixer ball, and 40 g of egg yolk, 35 g of granulated sugar, and 15 g of corn starch were sequentially added and mixed while stirring at a low speed. Furthermore, 320 g of cream cheese-like food (trade name: Creme Fromage, manufactured by Fuji Oil Co., Ltd.), egg yolk 40 g, and soft flour 20 g were added and mixed while stirring at low speed.
  • cream cheese-like food trade name: Creme Fromage, manufactured by Fuji Oil Co., Ltd.
  • Example b1 Production of acidic soybean material (whey non-separable type)
  • the soybean emulsified composition B obtained in Production Example 2 was used as a raw material before fermentation, and lactic acid fermented to produce a non-whey segregating type acidic soy material as described below.
  • the flavor of the acidic soybean material of this example was good with little miscibility and unpleasant taste and with a rich taste by fermentation. In addition, it was a loose paste, like Fromage Blanc, a kind of fresh cheese.
  • Example b2 Production of acidic soybean material (whey separation type) Using the soybean emulsified composition B obtained in Production Example 2 as a raw material before fermentation, lactic acid fermentation was performed to prepare a whey-separated type acidic soybean material as follows. did. In the same manner as in Example b1, lactic acid fermentation, pH adjustment and heat sterilization were performed, and then whey was separated and removed using a centrifuge, and the card was collected. The flavor of the obtained acidic soybean material had a good hardness like cream cheese, had little miscibility and unpleasant taste, and had a good taste due to fermentation.
  • Example b3 Production of acidic soybean material (whey separation type, added before phosphate separation) Lactic acid fermentation was carried out in the same manner as in Example b2, and sodium polyphosphate was adjusted to 0.2 at the time of pH adjustment using sodium hydroxide. %, And heat sterilization and separation of whey were performed in the same manner as in Example 2.
  • the flavor of the obtained acidic soy material has good hardness with cream cheese-like hardness, reduced roughness, less miscellaneous taste and unpleasant taste, and good taste with fermentation. It was.
  • Example b4 Production of acidic soybean material (whey separation type, added after phosphate separation) After performing lactic acid fermentation, pH adjustment, heat sterilization and whey separation in the same manner as in Example b2, kneading with a kneader While adding 0.5% sodium polyphosphate dissolved in a small amount of water, the temperature was raised to 85 ° C. in order to react the phosphate with the protein.
  • the flavor of the resulting acidic soy material is good with creamy cheese-like hardness, almost no roughness, little miscellaneous and unpleasant taste, and a rich taste from fermentation. Met.
  • Example b2 Acid soy material made from full-fat soymilk Full-fat soymilk “Unregulated soymilk” (Fuji Oil Co., Ltd.) was used as a fermentation raw material in the same manner as in Example b1, lactic acid fermentation, pH Adjustment and heat sterilization were performed. The flavor of the obtained acidic soybean material was also rough and bitter and unpleasant.
  • the dough ferry de PS8H (manufactured by Fuji Oil Co., Ltd.) was baked in an oven at 160 ° C and 160 ° C, and margarine "Message 500" (Fuji) What was made by heating and dissolving (Oil Manufacturing Co., Ltd.) was mixed.
  • 40 g of this was spread on a metal deco mold (diameter 12 cm). Pour 300g of cheese dough made of acidic soybean material on top of it, place the deco mold on a top plate with hot water at around 40 ° C, and bake around 30 minutes in an oven at 165 ° C on top and 165 ° C on bottom.
  • the top heat was raised to 190 ° C and baked for 5 minutes to obtain a baked cheese-like food with a beautiful baked color.
  • the obtained baked cheesecake-like food was visually inferior to that produced with cheese or filled type cheese, and became rich.
  • a new texture like that of a boiled rice cake was given, and a new cake different from ordinary baked cheese could be obtained.
  • Example c1 to c2, comparative example c1 Application to mayonnaise
  • Table 7 ingredients other than rice vinegar and oil were mixed and stirred with a homomixer, and rice vinegar was added thereto. Further, the oil was added in several portions while continuing to stir, and stirred sufficiently to prepare mayonnaise blended with the soybean emulsified composition B obtained in Production Example 2.
  • the control formulation was based on a formulation commonly used as a mayonnaise formulation.
  • the yolk substitution rates in Examples c1 and c2 and Comparative Example c1 were 50, 70, and 53%, respectively.
  • Table 8 shows the results of evaluation of the viscosity, texture and flavor of the mayonnaise prepared in each formulation.
  • the mayonnaise of Comparative Example c1 in which 53% of egg yolk was replaced with unadjusted soymilk had a reduced viscosity and a mayonnaise-like texture could not be obtained. Moreover, although the blue smell of soybean was hardly felt, it was not good because there was no richness and the flavor was too light.
  • Example c3 Application to dressing 3 g of water-soluble soybean polysaccharide (Soya Five-S; manufactured by Fuji Oil Co., Ltd.) was added to 280 g of water and dissolved while stirring. Next, 70 g of sugar, 70 g of soy sauce, 10 g of salt, 160 g of vinegar, and 7 g of bonito extract are added. After thoroughly stirring and mixing, 400 g of the soybean emulsified composition B obtained in Production Example 2 was gradually added to make the total amount 1000 g. Thereafter, the mixture was further homogenized with a homomixer, heated to 90 ° C., filled into a container, and a dressing was obtained. The obtained dressing had a rich taste and a good flavor.
  • Soya Five-S manufactured by Fuji Oil Co., Ltd.
  • Example c4 Application to sauce 100 g of mirin was placed in a pan and boiled over fire to make boiled mirin. Next, 500 g of water and 150 g of soy sauce were added, and when boiled, 20 g of shavings were added and simmered for a while. Thereafter, the fire was stopped and the mixture was cooled at room temperature. The shavings were scraped off with gauze to prepare a soup portion. 300 g of the soup part obtained by the above method and 700 g of the soybean emulsified composition A obtained in Production Example 1 were homogenized using a homomixer, heated to 90 ° C., and filled into a container to obtain a sauce. The obtained sauce had a smooth texture and a rich taste, and had a good flavor.
  • Example c6 Application to custard cream-like food (brown sugar combination) 200 g of soybean emulsified composition B obtained in Production Example 2, 15 g of flour, 15 g of fructose glucose liquid sugar (FC; manufactured by Oji Cornstarch Co., Ltd.), upper After mixing 32 g of white sugar, 5 g of oligotose (manufactured by Mitsubishi Chemical Foods), 5 g of brown sugar (manufactured by Ueno Sugar Co., Ltd.) and 48 g of water, the mixture was filtered once.
  • FC fructose glucose liquid sugar
  • the filtered product was put in a pan, heated, kneaded for 5 minutes after boiling, and water was added and mixed for the amount of evaporated water to prepare a custard cream-like food containing brown sugar.
  • the obtained custard cream-like food had a smooth texture, a rich taste, and a good flavor.
  • Example c7 Application to pudding-like food 28 g of whole egg, 14 g of sugar and 58 g of the soybean emulsified composition B obtained in Production Example 2 were mixed, heated at 80 ° C., and then baked in a hot water bath (150 ° C.). A pudding-like food was prepared. The obtained pudding-like food had good texture and flavor.
  • Example c8 Application to Bavaroa-like foods 92 g of soybean emulsified composition B obtained in Production Example 2 and 7 g of sugar were mixed, heated at 80 ° C., cooled to 40 ° C., and then swollen with a small amount of water in advance. 1 g of gelatin was added and cooled in a refrigerator for 4 hours to harden to prepare a bavaroa-like food. The resulting Bavaroa-like food had good texture and flavor.
  • the obtained brulee-like food had an appropriate hardness, a smooth texture and a good flavor.
  • Carbonara sauce was prepared by mixing 52 g of soybean emulsified composition A obtained in Production Example 1, 25 g of powdered cheese, 15 g of bacon, and 0.5 g of baking powder.
  • the obtained carbonara sauce had a rich taste and a good flavor. Also, when this sauce was mixed with boiled pasta, it was easy to mix with pasta and had a good flavor.
  • Example c11 Application to chawanmushi 50 g of soybean emulsified composition B obtained in Production Example 2, 25 g of egg, 120 g of soup stock and 1 g of salt are mixed, put into a mold, and steamed in a steamer at 85 ° C. for 15 minutes. Got. The resulting chawanmushi was moderately hard with a smooth texture and a good flavor.
  • Example c12 Application to mayonnaise Transglutaminase (trade name: Activa, manufactured by Ajinomoto Co., Inc.) was added to the soybean emulsion composition B obtained in Production Example 2 at 0.5% (soy emulsion). 2.7% based on the solid content of composition B), react at 55 ° C. for 30 minutes, and then stop at 90 ° C. for 30 minutes to stop the reaction and obtain an egg yolk substitute composition in which transglutaminase is allowed to act It was.
  • this egg yolk substitute composition 40 g of this egg yolk substitute composition, 0.7 g of powdered mash, 3 g of salt, 0.25 g of sodium glutamate, 8 g of apple vinegar and acetic acid mixed to adjust the acidity to 10%, 8 g of water are mixed, and 140 g of rapeseed oil is added to the food.
  • Mayonnaise was prepared by adding with stirring with a cutter. The mayonnaise oil content was 70%. This mayonnaise was prepared by substituting egg yolk for 100% with egg yolk substitute composition, but the emulsified state is very good and stable, there is no special flavor like pickled soybean, and the rich taste There was good flavor.
  • Example c13 Application to custard cream-like food 4 parts by weight of white chocolate (trade name: White Coaching YDV36, manufactured by Fuji Oil Co., Ltd.) was dissolved in a hot water bath at 50 ° C. A custard cream-like food was prepared by adding 3 parts by weight of the soybean emulsified composition B obtained in Production Example 2 to the white chocolate removed from the hot water bath while gently stirring. The obtained custard cream-like food had a smooth texture, a rich taste, and a good flavor without the blue smell of soybeans.
  • white chocolate trade name: White Coaching YDV36, manufactured by Fuji Oil Co., Ltd.
  • Example d1 Tofu 2 Add 80 parts of bittern to 80 parts of soybean emulsified composition B (dry matter content 18.2%) obtained in Production Example 2, mix well, fill into a container, cook in a steamer at 95 ° C for 25 minutes, Cooled to obtain tofu.
  • the obtained tofu was not as hard and brittle as the tofu of Comparative Example d1, but had a soft and crisp physical property.
  • the mouthfeel is creamy and melts well in the mouth, rather than tofu, with a creamy pudding with a smooth mouthfeel, or a rich mouthfeel like Ankimo (ancho liver) there were.
  • the flavor was clearly different from the tofu flavor of Comparative Example d1, and had a richer and better soybean flavor. It was very delicious when ponzu soy sauce was applied to the obtained tofu of the fourth invention.
  • Example d2 Tofu 3
  • 8 parts, 32 parts, and 48 parts of soy milk were replaced with the soybean emulsified composition B of Production Example 2, and the substitution rate of soy milk was 10%, 40%, and 60%, respectively, in the same manner.
  • Got tofu. The obtained tofu had a richer flavor as the substitution rate was higher, and became a texture with a creamy feeling.
  • Example d3 Soy seasoning Soy emulsified composition B obtained in Production Example 2 was mixed with 75 parts of ponzu soy sauce and mixed well to obtain a soy seasoning. When this seasoning was eaten over sashimi, it had a creamy, mellow acidity and richness, an appearance color and viscosity similar to sesame seeds, and a very good flavor compatibility with sashimi.
  • Example d4 Yuba
  • the soybean emulsion composition B obtained in Production Example 2 was filled in a flat tray to a height of 2 cm and frozen in a freezer at ⁇ 20 ° C. for 10 days. When this was thawed, it became a fine yuba piece, and when it was eaten, it had a thick texture and a texture and flavor of kumage yuba. When seasoning liquid was added to this, it was able to eat more deliciously.
  • soymilk was adjusted to a solid content concentration of 12% at a vacuum pressure of 100 torr with a concentrator (manufactured by Nisaka Manufacturing Co., Ltd., hereinafter the same) to obtain concentrated soymilk.
  • this concentrated soymilk was adjusted to 75 ° C., and 100 parts of the concentrated soymilk, 3 parts of waxy corn starch “Delica SE” (manufactured by Nissho Chemical Co., Ltd.) and dextrin “Sandeck 250” (Sanwa Starch) 2 parts of Kogyo Kogyo Co., Ltd.
  • Example d5 and d6 Frozen tofu
  • the concentrated soy milk obtained in Reference Example d1 was obtained by adding the soybean emulsified composition B (solid content concentration 18.2%) obtained in Production Example 2 to the concentrated soy milk: soy emulsified composition.
  • Product B 70: 30 (Example d5), 40:60 (Example d6) were mixed at a ratio and prepared in the same manner as in Reference Example d1 to obtain frozen tofu.
  • Reference Example d2 Frozen deep fried (control)
  • the tofu before freezing was prepared in the same manner as in Reference Example d1 except that the soymilk solid content concentration was 16%.
  • This tofu was fried in rapeseed oil at 180 ° C. for 2 minutes, and then snap-frozen in an atmosphere of about ⁇ 35 ° C. to obtain a frozen deep-fried (control).
  • Example d7 to d9 Frozen deep-frying
  • the concentrated soy milk obtained in Reference Example d2 is obtained by adding the soy emulsion composition B (solid content concentration 18.2%) obtained in Production Example 2 to the concentrated soy milk: soy emulsion.
  • Composition B 85: 15 (Example d7), 70:30 (Example d8), and mixed at a ratio of 40:60 (Example d9), prepared in the same manner as Reference Example d1, and before freezing Tofu was prepared.
  • This tofu was fried in rapeseed oil at 180 ° C. for 2 minutes, and then snap-frozen in an atmosphere of about ⁇ 35 ° C. to obtain a deep-fried deep-fried chicken.
  • the texture was sensory evaluated after thawing frozen tofu and deep-fried to room temperature. A smooth and tofu-like texture was scored 5 points, a hard and poorly melted mouthfeel, and a grainy texture due to the addition of starch was scored 1 point. As for the flavor, 5 points were given to feel the preferred bean flavor, and 1 point was given to those that did not feel the bean taste. Evaluation was performed by a five-point scoring method with five panelists.
  • Reference Example d1 had a light flavor with little rich soybean flavor. Moreover, the texture was hard and was the same texture as the conventional tofu.
  • soy emulsified composition B 70: 30 in Example d5
  • the rich (bright) flavor of soybean is felt compared to Reference Example d1
  • it does not smell blue and the texture is The texture was soft and smooth with good mouth melting, and the tofu grains melted when chewed.
  • soy emulsified composition B 40: 60 in Example d6
  • a thicker soybean flavor is felt compared to Example d5
  • the texture is very soft like pudding. It was smooth and melted well in the mouth, with a creamy texture.
  • soy emulsified composition B 40: 60 in Example d9
  • the rich soybean flavor is more strongly felt compared to Example d8, and the texture is very soft and smooth and melts in the mouth. was also very good and had a very creamy texture.
  • the substitution rate of the soy emulsion composition B with respect to soy milk is preferably 15% or more, and more preferably 20% or more.
  • a rich soybean flavor was imparted, and a soft and smooth texture was achieved.
  • the dough was formed into a diameter of 58 mm, a thickness of 10 mm, and one piece of 27 g with a molding machine. This is heated in the low temperature part (92 to 135 ° C) for 8 minutes and in the high temperature part (155 to 180 ° C) for 1 minute and 30 seconds. After air cooling, it is rapidly frozen at -35 ° C to obtain frozen cancer. It was.
  • Example d10 Cancer pounding 2 (with soybean emulsified composition B)
  • water was completely replaced with the soybean emulsified composition B obtained in Production Example 2, and the others were similarly obtained.
  • the resulting cancer mushroom has a sponge-like structure inside, is larger than conventional cancer mushrooms, maintains a volume even after boiling, contains a seasoning liquid, and has an unprecedented juicy mouthfeel. Had a texture. In addition, the flavor had the original richness of soybeans.
  • Cancer return 3 (change in blending amount of soybean emulsified composition B) Proteins contained in dough by blending 142 parts, 355 parts, 710 parts and 1420 parts of soybean emulsified composition B obtained in Production Example 2 in the dough preparation method of Reference Example d3 per dough preparation The amount of water was adjusted so that the amounts were equal, and in the same manner, the other cancers were finally obtained in the same manner with a water substitution rate of 10%, 25%, 50%, and 100% (Examples d11 and d12). , D13, d14). As for the obtained cancer mushroom, as the blended amount of the soybean emulsified composition B was increased, the volume after fried food was increased, and a cancer mushroom that contained a seasoning liquid was obtained.
  • the obtained cancer squirrel was measured by visually observing the degree of swelling of the dough and measuring the long side (X), short side (Y), and thickness (H) with a caliper.
  • the volume was calculated from mm), and the expanded state after the frying was confirmed.
  • the volume (V) was calculated as V ⁇ R 2 ⁇ 3.14 ⁇ H, where 1 ⁇ 2 of the average value of the long side and short side was the radius (Rcm).
  • the numerical value was expressed as a ratio (%) when the volume of Reference Example 3 was 100.
  • the substitution rate of the soy emulsified composition B with respect to water is 10% or more. If it is preferably 25% or more, more preferably 50% or more, the shape after frying has a thickness and a cancerous rejuvenation can be obtained. The inside had a fine sponge-like structure, and after boiling it well contained the seasoning liquid, and it was possible to obtain a succulent and thrilling cancer.
  • Example d15 Deep-fried oil 2 (containing soybean emulsified composition B)
  • Reference Example d4 fried chicken was obtained in the same manner as Reference Example d4 except that 760 parts of 1550 parts of water were replaced with the soybean emulsified composition B obtained in Production Example 2.
  • the obtained fried chicken had a plump appearance and the inside was a fine texture. Compared to conventional fried chicken, it has a flavor with a rich soybean-derived taste, and when seasoned with a seasoning liquid, the seasoning liquid is well absorbed and a juicy seasoned fried food is obtained.
  • This fried chicken was further fried and drained so that the weight of the seasoning liquid was 1: 1, and then heat-dried to obtain a deep-fried fried chicken with a volume (thickness) close to that before seasoning.
  • the seasoned dry fried chicken was well returned to the hot water, and a deep-fried fried chicken well containing the seasoning liquid was obtained.
  • the resulting deep-fried fried food was measured by visually observing the degree of expansion of the dough, then measuring the size of the long side, short side, and thickness with calipers, calculating the volume from the average value of 5 pieces (mm), The swelling state of was confirmed. The numerical value was expressed as a ratio (%) when the volume of Reference Example 4 was 100. And the obtained fried chicken was boiled for about 3 minutes with a Japanese-style seasoning liquid, then taken out and weighed. And the absorptivity of the fried seasoning liquid was measured by the ratio (%) of the weight after cooking to the weight before cooking. Furthermore, the flavor, shape (bulge) and liquid absorbency of fried chicken were evaluated in five levels: (++++) very good, (++) good, (+) slightly good, ( ⁇ ) normal, and ( ⁇ ) bad.
  • the substitution rate of the soy emulsified composition B with respect to water is 10% or more. If it is preferably 25% or more, more preferably 50% or more, the shape after frying has a thickness, and a deep fried chicken can be obtained. The inside had a fine sponge-like structure, and after boiling it well contained the seasoning liquid, and a fried chicken with a juicy taste could be obtained.
  • Example e1 Serum cholesterol reduction test Serum cholesterol was measured by feeding a rat with the formulation shown in Table 13 to rats.
  • Example e1 Serum cholesterol concentration is significantly lower in the test group using soybean emulsified composition B (Example e1) after 2 weeks of feeding than in the test group using Casein (Comparative Example e1) showed that.
  • the serum cholesterol concentration in the rat at the start of the test was 144.7 ⁇ 4.7 mg / dl (mean value ⁇ SE).
  • Zucker fatty rats are mutants exhibiting marked obesity, and it is known that serum cholesterol levels increase with age.
  • Comparative Example e1 shows that serum cholesterol elevation associated with aging in Zucker fatty rats does not inhibit any casein-based diet, whereas Example e1 actively promotes serum cholesterol elevation associated with aging in Zucker fatty rats. Has been shown to be suppressed.
  • Example f1 Urinary NAG activity reduction test Rats were fed with the diet shown in Table 14, and NAG ( ⁇ -N-acetyl-D-glucosaminidase) in urine was measured. NAG is widely distributed in organs, but since it is a fairly large enzyme, serum enzymes rarely appear in urine. By quantifying the amount of NAG in urine, degeneration and destruction of tubular epithelial cells You can know such injuries. (Table 14) Feed formulation
  • Example f1 Test result The urinary NAG activity showed a significantly lower value in the test group using soybean emulsified composition B by feeding for 2 weeks compared to the test group using Casein.
  • the Zucker fatty rat is a mutant exhibiting remarkable obesity, and nephropathy is known to occur as a complication with aging.
  • Comparative Example f1 it was shown that the occurrence of nephropathy accompanying aging in Zucker fatty rats was not suppressed at all by Example f1, while it was shown that it was positively suppressed.
  • the first to fifth inventions can be used for the production of various foods in which soybean-derived materials are used as raw materials for various purposes.
  • soybean-derived materials are used as raw materials for various purposes.
  • a part or all of dairy products such as milk, fresh cream, whole milk powder, etc.
  • a greatly improved soybean emulsified composition a food-derived food containing a soybean-derived material with a significantly improved flavor can be provided.
  • a consumer can reduce serum cholesterol or improve renal function through eating habits. Therefore, in the food industry, it is possible to develop products that stimulate new demand.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Agronomy & Crop Science (AREA)
  • Botany (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dairy Products (AREA)
  • Beans For Foods Or Fodder (AREA)

Abstract

The present invention addresses the problem of providing a soybean-derived raw material-containing food or beverage that improves problems with flavor and physical properties, such as the grassy smell caused by soybean raw materials, and markedly improves product quality, in a soybean-derived raw material-containing food or beverage using conventional soybean raw materials such soymilk or tofu. Provided are a milk-substitute composition, and an egg-yolk substitute composition, etc., characterized by including a soybean emulsion composition having a protein content relative to dry material of at least 25 wt%, a fat content (as a chloroform/methanol mixed solvent extract) relative to the protein content of at least 100 wt%, and an LCI value of at least 55%. Also provided are a variety of soybean-derived raw material-containing food and beverages using these compositions.

Description

新規な大豆乳化組成物の大豆由来原料含有飲食品への用途Use of a novel soybean emulsified composition for foods and drinks containing soybean-derived ingredients
 本発明は、大豆素材を原料として各種用途で使用した大豆由来原料含有飲食品に関するものである。より具体的には、本発明は、大豆由来原料を使用した洋菓子類、パン類、デザート類、飲料類、スープ類、ソース類などの大豆由来原料含有飲食品、並びにそれらの製造法に関する。また本発明は酸性大豆素材及びこれを用いた洋菓子等の種々の食品に関する。特にヨーグルト類やフレッシュチーズなどのチーズ類と同様の用途に適した酸性大豆素材に関する。また本発明は、卵黄代替組成物及びそれを使用した食品に関するものである。また、本発明は豆腐等の大豆加工食品、大豆加工食品製造用の食感もしくは風味の改良剤、及び、大豆加工食品の食感もしくは風味の改良方法に関する。特に、新しい食感と風味を有する大豆加工食品、あるいは従来の品質を改良した大豆加工食品に関する。また本発明は、血清コレステロール低減用もしくは腎機能改善用組成物に関する。 The present invention relates to a soy-derived raw material-containing food or drink using a soy material as a raw material in various applications. More specifically, the present invention relates to foods and beverages containing soybean-derived materials such as confectionery, breads, desserts, beverages, soups, sauces and the like using soybean-derived materials, and methods for producing them. Moreover, this invention relates to various foodstuffs, such as an acidic soybean raw material and western confectionery using the same. In particular, the present invention relates to an acidic soybean material suitable for uses similar to cheeses such as yogurts and fresh cheeses. The present invention also relates to an egg yolk substitute composition and a food using the same. The present invention also relates to a processed soybean food such as tofu, a texture or flavor improving agent for manufacturing processed soybean food, and a method for improving the texture or flavor of processed soybean food. In particular, the present invention relates to a processed soybean food having a new texture and flavor, or a processed soybean food having improved conventional quality. The present invention also relates to a composition for reducing serum cholesterol or improving renal function.
 豆乳や豆腐、分離大豆蛋白などの大豆素材は、飲食品において乳製品の代替原料、発酵乳製品の代替原料、卵黄の代替原料、大豆加工食品の原料、血清コレステロール低減用の原料及び腎機能改善用の原料など、様々な飲食品用途で原料として使用されている。 Soy materials such as soy milk, tofu and isolated soy protein are used as alternative raw materials for dairy products, alternative raw materials for fermented milk products, alternative raw materials for egg yolks, raw materials for processed soybean foods, raw materials for reducing serum cholesterol, and kidney function improvement It is used as a raw material in various food and drink applications such as raw materials for food.
○食品における乳製品の代替に関する技術
 洋菓子、パン、デザートなどのトッピング(飾り付け)やナッペ(表面コーチング)、フィリング、練り込みなどの用途に、また調理用の風味付け用途に、生クリームやコンパウンドクリーム(脂肪分が乳脂肪と植物性油脂を混合されたもの)が独特のコクのある乳風味付与や冷涼感のある口当たり付与のために幅広く利用されている。特に、生クリームは濃厚なコク味、乳味のため洋菓子や調理に欠かせない素材であるが、比較的高価であること、冷蔵保管でも開封後は2~3日以内に使用する必要があり長期保存が難しいこと、加えて油分が30~50重量%と比較的高油分でありヘルシー指向に合致しにくいことから、風味が良くて使い勝手の良い生クリームを代替しうる素材が求められている。
○ Technology related to substitution of dairy products in foods Fresh cream and compound cream for topping (decoration), nappe (surface coating), filling, kneading, etc. for confectionery, bread, dessert, etc. (Fat is a mixture of milk fat and vegetable oil) is widely used for imparting a unique, rich milk flavor and a cool mouthfeel. In particular, fresh cream is an indispensable ingredient for pastry and cooking due to its rich body and milky taste, but it is relatively expensive and must be used within 2-3 days after opening even in refrigerated storage. Because it is difficult to store for a long time, and because the oil content is relatively high (30-50% by weight) and it is difficult to match healthy orientation, there is a need for a material that can replace fresh cream with good flavor and ease of use. .
 上記を代替しうる素材として、豆腐や豆乳の利用が検討されているが、かかる原材料を使用した洋菓子類、デザート類、飲料類、スープ類、ソース類などの大豆由来原料含有飲食品は、ヘルシーなイメージはあるものの、大豆に由来する特有の青臭みを持つとともにコク味、旨味に乏しいものであった。そのため、かかる風味が改善された大豆由来原料含有飲食品に対する市場ニーズは高いものである。 The use of tofu and soy milk is being considered as a material that can replace the above, but foods and beverages containing soy-derived ingredients such as confectionery, desserts, beverages, soups and sauces using such ingredients are healthy. Although there was a good image, it had a peculiar blue odor derived from soybeans, and it had poor body and umami. Therefore, the market needs for food and drink containing soybean-derived raw materials with improved flavor are high.
 上記の大豆由来の特有の風味を改善する方法として、特許文献1には、大豆飲食物に対して分岐構造を有する3~4糖類を含有する低甘味の糖組成物を添加して、大豆由来の青臭みを抑制するとともに加熱処理工程で生じるざらつき感も抑制する方法が開示されている。特許文献2には、豆乳又は豆乳の製造工程中の大豆原料に蛋白質架橋酵素を作用させ、次いで滅菌処理する工程を含むことを特徴として得られる濃厚感(コク味)と良好な風味を有する滅菌豆乳が開示されている。また、特許文献3には、豆乳を130~150℃で数十秒間加熱処理した後、乳酸発酵や酸性物質添加で酸性にすることを特徴とする酸性豆乳飲料に関し、大豆特有の青臭み、エグ味などの不快な風味がなく、飲み口がスッキリとし、且つコク味のあるという酸性豆乳飲料の製造法が開示されている。 As a method for improving the above-mentioned unique flavor derived from soybeans, Patent Document 1 adds a low-sweet saccharide composition containing 3 to 4 saccharides having a branched structure to soybean foods and beverages, and is derived from soybeans. A method for suppressing the blue odor of the heat treatment and suppressing the rough feeling produced in the heat treatment step is disclosed. Patent Document 2 discloses a sterilization having a rich feeling (kokumi) and a good flavor characterized by including a step of allowing protein cross-linking enzyme to act on soy milk or a soybean raw material in the production process of soy milk, and then sterilizing. Soymilk is disclosed. Patent Document 3 relates to an acidic soymilk beverage characterized in that soymilk is heat-treated at 130 to 150 ° C. for several tens of seconds and then acidified by lactic acid fermentation or addition of an acidic substance. There is disclosed a method for producing an acidic soy milk drink that has no unpleasant taste such as taste, has a refreshing mouth and has a rich taste.
○食品における発酵乳製品の代替に関する技術
 次に、乳原料を乳酸発酵させた発酵乳は、チーズ類やヨーグルト類としてそのまま食され、また他の洋菓子、和菓子、パン、デザート、冷菓、飲料等の加工食品における主原料あるいはトッピング用(飾り付け)、練り込み用、フィリング用、ナッペ用(表面コーチング)などの副原料として利用されている。
 また、乳蛋白質として脱脂粉乳等を用いる一方で、乳脂の代わりに植物性油脂を配合し、乳酸発酵させて製造されたフィルドミルクタイプの発酵乳化物が知られている(特許文献4~7)。この乳化物も上記加工食品へ発酵乳と同様の用途で使用され、様々な機能性及び付加価値を加工食品に付与し、食品産業の発展に寄与している。
○ Technology related to substitution of fermented dairy products in foods Next, fermented milk made by lactic fermentation of milk ingredients is eaten as cheeses and yoghurts as well as other Western confectionery, Japanese confectionery, bread, dessert, frozen confectionery, beverages, etc. It is used as a main ingredient in processed foods or as an auxiliary ingredient for topping (decoration), kneading, filling, nappe (surface coating).
Further, while using skim milk powder or the like as milk protein, a filled milk type fermented emulsion produced by blending vegetable oil and fat in place of milk fat and subjecting it to lactic acid fermentation is known (Patent Documents 4 to 7). . This emulsion is also used for the processed food in the same application as fermented milk, and imparts various functions and added values to the processed food, contributing to the development of the food industry.
 一方、蛋白源として大豆蛋白質を利用した発酵大豆素材は、乳脂を使用しないため、健康に良い植物性の発酵素材として期待されており、一般的な発酵乳製品であるチーズやヨーグルトとの代替を目的として一部検討されている。大豆蛋白質を含有する原料としては、豆乳や分離大豆蛋白、大豆粉などの大豆素材が使用されている。 On the other hand, fermented soy materials that use soy protein as a protein source do not use milk fat, so they are expected to be plant-based fermented materials that are good for health, and can be used as a substitute for general fermented dairy products such as cheese and yogurt. Some have been studied as a purpose. As raw materials containing soy protein, soy materials such as soy milk, separated soy protein, and soy flour are used.
○食品における卵黄の代替に関する技術
 次に、卵黄はその機能、栄養価等に優れた特徴を有することから、調味料、惣菜、菓子など様々な食品に幅広く利用されている。しかし、近年、消費者の健康への意識が高まっていることから、コレステロール含量の高い卵の摂取が敬遠される傾向にある。また、元来「物価の優等生」と言われてきた鶏卵であるが、近年災害や鳥類感染症などの影響により、鶏卵価格の上昇や、供給の不安定等が懸念される。
○ Technology related to substitution of egg yolk in foods Next, egg yolk has been widely used in various foods such as seasonings, sugar beets, and confectionery because it has excellent features such as function and nutritional value. However, in recent years, consumers' awareness of health has increased, and there is a tendency to avoid eating eggs with high cholesterol content. In addition, chicken eggs that were originally said to be “price-preferred students” are concerned, however, due to the effects of disasters and avian infectious diseases in recent years, there are concerns about rising egg prices and unstable supply.
 このような背景から、これまで卵代替食品に関する検討が種々為されてきた。例えば、乾燥卵白粒子とカボチャの乾燥黄色粒子を使用したタマゴ様食品とその使用例として玉子焼きが提案されている(特許文献9)。また、大豆原料を使用した技術として、大豆タンパク質と卵白タンパク質等を含む低コレステロールの卵代替組成物とその応用例としてベーカリー製品(パウンドケーキ)に使用する技術(特許文献10)や、濃縮豆乳を含む乳化物とその応用例としてマヨネーズ様食品に使用する技術(特許文献11)が開示されている。また、単離ホエー蛋白質を使用した卵代替食品の製造方法とスポンジケーキやカスタードプリン等に応用した技術が開示されている(特許文献12)。 From this background, various studies on egg substitute foods have been made. For example, egg-like foods using dried egg white particles and pumpkin dried yellow particles, and egg-yaki as an example of use thereof have been proposed (Patent Document 9). In addition, as a technique using soy materials, a low cholesterol egg substitute composition containing soy protein and egg white protein, and a technique (Patent Document 10) used for bakery products (pound cake) as an application example, concentrated soymilk The technique (patent document 11) used for the mayonnaise-like food is disclosed as an emulsion containing and the example of application. Moreover, the manufacturing method of the egg substitute food using isolated whey protein and the technique applied to sponge cake, custard pudding, etc. are disclosed (patent document 12).
○大豆加工食品に関する技術
 次に、大豆を原料とした豆腐や湯葉、油揚げなどは日本において古くから食されてきた伝統的な大豆加工食品である。一般的には大豆に水を加えて磨砕し、得られた呉(大豆スラリー)を必要により加熱し、遠心分離や濾過等によりオカラを分離して得られた「豆乳」を出発原料として、様々な大豆加工食品が製造されている。豆腐は大豆加工食品の典型例であり、豆乳をニガリなどの凝固剤で固める工程を経て、各種の豆腐が製造されている。凝固剤で固まったカードを切断し、水にさらしたものは「絹ごし豆腐」と呼ばれており、さらにカードを崩しながら濾布で脱水し、成型したものが「木綿豆腐」と呼ばれている。これらの豆腐は比較的しっかりとした保形性のあるゲルで、近年はプラスチック容器に密封された充填豆腐として販売されており、それぞれの硬さは異なるが、いずれも適度な硬さとさっくりとしたゲルの食感を有する点で共通する。これらの伝統的な豆腐は永く好まれて日本人の食生活に欠かせないものとなっているが、一方で産業的には安価で利益率が低いため、伝統的な豆腐と差別化された高付加価値の新しい豆腐が切望されている。そこで最近ではカードを成型せずに直接容器に盛り、カードの組織を一部壊した不定形の形状にした寄せ豆腐が販売されている。
 また、豆腐に限らず、その他の油揚げやがんもどき、厚揚げ、湯葉等の大豆加工食品においても、安価な従来品と差別化された高付加価値の新しい品質の製品を開発し、提供することが求められている。
○ Technology related to processed soybean foods Next, tofu, yuba and fried chicken made from soybeans are traditional processed soybean foods that have been eaten for a long time in Japan. In general, water is added to soybean and ground, and the resulting kure (soybean slurry) is heated as necessary, and the soymilk obtained by separating the okara by centrifugation or filtration is used as a starting material. Various processed soybean foods are manufactured. Tofu is a typical example of processed soybean food, and various types of tofu are produced through a process of solidifying soy milk with a coagulant such as bittern. A piece of curd curd that has been cut with a coagulant and exposed to water is called “silk tofu”, and then the card is dehydrated with a filter cloth while breaking down the card, and the molded one is called “cotton tofu” . These tofu are relatively solid gels that have been sold as filled tofu sealed in plastic containers in recent years. This is common in that it has a texture of gel. These traditional tofu have long been preferred and have become an indispensable part of the Japanese diet. On the other hand, they are differentiated from traditional tofu because they are industrially cheap and have low profit margins. New value-added tofu is eagerly desired. Therefore, recently, tofu that has been placed in a container directly without molding the card and has an irregular shape that partially destroys the card structure has been sold.
In addition to tofu, other high-value-added new quality products that are differentiated from cheap conventional products are also developed and provided for other deep-fried soy processed foods such as deep-fried fried chicken, fried cancer, deep-fried chicken, and yuba. It is demanded.
 かかる状況下、上記の市販の豆腐に対して、様々な性状の豆腐の開発が試みられている。例えば、特許文献13には、豆腐に増粘ゲル化剤とオリゴ糖や澱粉を混合し、高速カッターで撹拌し、加熱冷却して得られる豆腐ペーストが記載されている。また、特許文献14には、水相部にペースト状に加工した豆腐を添加し、油中水型に乳化してなる豆腐含有油脂組成物が記載されている。
 また、特許文献15には、加熱処理してNSIを低下させた大豆から豆乳を調製し、これを酸沈して蛋白質を濃縮し、さらに中和して蛋白質を再溶解させた後に凝固剤を加えて加熱凝固させ、クリーム様テクスチャーを有する大豆蛋白質カードを製造することが記載されている。
Under such circumstances, development of tofu with various properties has been attempted with respect to the above-mentioned commercially available tofu. For example, Patent Document 13 describes a tofu paste obtained by mixing a thickening gelling agent, oligosaccharide or starch with tofu, stirring with a high-speed cutter, and heating and cooling. Patent Document 14 describes a tofu-containing oil / fat composition obtained by adding tofu processed into a paste to the aqueous phase and emulsifying into a water-in-oil type.
In Patent Document 15, soy milk is prepared from soybeans that have been heat-treated to reduce NSI, and this is acid precipitated to concentrate the protein, and further neutralized to redissolve the protein, and then add a coagulant. In addition, it is described that a soybean protein curd having a cream-like texture is produced by heat coagulation.
○血清コレステロールの低減及び腎機能の改善に関する技術
 次に、大豆を原料とする血清コレステロール低減用組成物に関連する出願としては、特許文献16が存在する。また、血中コレステロール低下粉末素材に関する出願としては、特許文献17が存在する。分画された大豆たん白素材に関連する出願として、特許文献18が存在する。また、大豆を原料とする、腎症患者用大豆たん白素材については、特許文献19に記載がある。
Technology for reducing serum cholesterol and improving renal function Next, Patent Document 16 is present as an application related to a composition for reducing serum cholesterol using soybean as a raw material. Further, Patent Document 17 exists as an application related to a blood cholesterol-lowering powder material. As an application related to the fractionated soybean protein material, Patent Document 18 exists. Patent Document 19 describes a soy protein material for nephropathy patients using soybean as a raw material.
特開2006-280310号公報JP 2006-280310 A 特開2004-261107号公報JP 2004-261107 A 特開2007-159593号公報JP 2007-159593 A 特開平11-9175号公報Japanese Patent Laid-Open No. 11-9175 特開平9-313101号公報JP-A-9-313101 特開2000-279121号公報JP 2000-279121 A 特開2004-357522号公報JP 2004-357522 A 国際公開WO2006/135089号パンフレットInternational Publication WO2006 / 135089 Pamphlet 特開平8-298963号公報JP-A-8-298963 特表2007-521824号公報Special table 2007-521824 特開2002-34503号公報JP 2002-34503 A 特開平2-42942号公報JP-A-2-42942 特開2002-209号公報Japanese Patent Laid-Open No. 2002-209 特開2003-38096号公報JP 2003-38096 A 特公昭61-3462号公報Japanese Patent Publication No.61-3462 特開2003-088334号公報JP 2003-088334 A 特開2009-280517号公報JP 2009-280517 A 国際公開WO2006/129647号パンフレットInternational Publication WO2006 / 129647 Pamphlet 国際公開WO2009/110504号パンフレットInternational Publication WO2009 / 110504 Pamphlet
 本発明は、従来の豆乳や豆腐などの大豆素材を使用した大豆由来原料含有飲食品において、該大豆素材に由来する青臭みなどの風味や物性面における欠点を改良し、品質を格段に向上させた大豆由来原料含有飲食品を提供することを課題とする。以下、具体的な課題を挙げる。 In the present invention, food and drink containing soy-derived ingredients using conventional soybean materials such as soy milk and tofu, the defects in flavor and physical properties such as blue odor derived from the soybean material are improved, and the quality is remarkably improved. An object is to provide food and drink containing soybean-derived materials. Specific issues are listed below.
○第一の課題
 食品における乳製品の代替に関する技術について、特許文献1の方法では、低甘味の糖組成物特有の風味が自然な大豆風味とやや違和感があるとともに、大豆由来の青臭みは抑制できるものの濃厚なコク味には欠けるものであった。豆乳に蛋白質架橋酵素を作用させ、次いで滅菌処理するという特許文献2の方法では、青臭み、コク味とも改善出来る方法ではあるが、酵素反応条件の微妙な制御が必要で容易でないという問題があった。また、特許文献3の酸性豆乳飲料は、青臭みがなくコク味のあるものではあるが、酸性風味であることから利用用途が飲料や調理用などに限定されるという問題があった。
 上記のように、従来のいずれの方法も、生クリームなどの乳製品を代替しうるような、大豆由来の青臭みがなく濃厚なコク味のある大豆由来素材として十分なものとはいえなかった。
 そこで本発明は、生クリームなどの乳製品代替物として使用することができる風味の改善された大豆乳化組成物、及びその使用により、大豆由来の青臭みがなく濃厚なコク味のある大豆由来原料含有飲食品を提供することを課題の一つとする。
○ First issue Regarding the technology related to the replacement of dairy products in foods, the method of Patent Document 1 has a slightly uncomfortable feeling that is unique to the low-sweet sugar composition and a natural soy flavor, and suppresses the blue odor derived from soybeans. Although it was possible, it was lacking in the rich body. The method of Patent Document 2 in which a protein cross-linking enzyme is allowed to act on soy milk and then sterilized is a method that can improve both blue odor and body taste, but has a problem that delicate control of enzyme reaction conditions is necessary and is not easy. It was. Moreover, although the acidic soymilk drink of patent document 3 does not have a blue odor and has a full-bodied taste, there is a problem that the usage is limited to beverages and cooking because of its acidic flavor.
As described above, none of the conventional methods can be said to be sufficient as a soy-derived material having a rich and rich taste without a blue odor derived from soy that can replace dairy products such as fresh cream. .
Accordingly, the present invention provides a soy emulsified composition with improved flavor that can be used as a substitute for dairy products such as whipped cream, and a soy-derived raw material that does not have a blue odor derived from soy and has a rich body taste. One of the problems is to provide the contained food and drink.
○第二の課題
 食品における発酵乳製品の代替に関する技術について、製菓・製パン分野における乳の持つコクと爽やかな風味に対する嗜好性は非常に高いものであったため、乳原料と前記大豆素材との置き換えによって植物性発酵素材が当該分野に入り込む余地は従来殆どなかったと言える。また発酵大豆素材もそれ自体の風味で乳風味の持つコクや爽やかさを有するものではなく、大豆独特の渋味や収斂味といった好ましくない風味を感じやすい。さらに、大豆独特の保水性やゲル化性といった物性がヨーグルトやチーズの物性とは異質なものであり、菓子やパンの品質に影響しやすく、さらに乳素材との代替使用への障壁を高くしていた。
○ Second issue Regarding the technology related to the substitution of fermented dairy products in foods, the preference for the richness and refreshing flavor of milk in the confectionery and bakery field was very high. It can be said that there has been little room for the plant fermentation material to enter the field by replacement. In addition, fermented soy materials are not flavorful and refreshing with a milky flavor in their own flavor, and it is easy to feel unpleasant flavors such as astringent taste and astringent taste peculiar to soybeans. In addition, the water retention and gelation properties unique to soybeans are different from those of yogurt and cheese, which can easily affect the quality of confectionery and bread, and raise the barrier to alternative use of dairy ingredients. It was.
 従来の発酵大豆素材の問題を改良すべく、フレーバー等によるマスキング、他素材との混合、プロテアーゼ処理、トランスグルタミナーゼ処理、乳酸菌の種類の選択、乳酸発酵の条件の最適化等の様々なアプローチが採られて来た(特許文献8など)が、未だ製菓・製パン分野への使用に耐えうる発酵大豆素材は限定的である。
 そこで本発明は、発酵乳やフィルドタイプの発酵乳化物が持つようなコクのある風味を有し、大豆の好ましくない風味も感じにくく、かつ滑らかな食感も併せ持つ酸性大豆素材を提供することを課題の一つとする。
Various approaches such as masking with flavor, mixing with other materials, protease treatment, transglutaminase treatment, selection of the type of lactic acid bacteria, optimization of lactic acid fermentation conditions, etc. are adopted to improve the problems of conventional fermented soybean materials. However, fermented soybean materials that can withstand use in the field of confectionery and bakery are still limited.
Therefore, the present invention provides an acidic soy material that has a rich flavor like fermented milk and filled type fermented emulsions, has an unfavorable taste of soybean, and has a smooth texture. One of the issues.
○第三の課題
 食品における卵黄の代替に関する技術について、特許文献9の技術では、卵黄の代わりにカボチャの黄色粒子を配合しているのみで、卵黄代替食品に要求されるコク味を付与できるものではなかった。また、特許文献10の技術では原料として用いられている大豆蛋白質に由来する青臭みのある風味が製品の風味に影響を及ぼす場合があり、また、卵白蛋白質が必要となるため、鶏卵の供給の不安定性の影響を受ける場合がある。また、特許文献11の技術では豆乳を濃縮することにより豆乳の不快臭の低減を図っているものの、風味において必ずしも満足できるものではなく、また、マヨネーズ様食品のコク味等の品質面においてもまだ改善する余地が有った。特許文献12の技術では、使用するホエー蛋白質をイオン交換法の吸着樹脂によって高純度にしなければならず、製造上の制約を受ける上、高価である問題があった。そのため、コクがあって、風味が良く、安価で供給可能な卵黄代替物が望まれている。
 そこで本発明は、コクがあって風味が良く、卵黄と比較すると安価であり安定的に供給可能な卵黄代替組成物及びそれを使用した食品を提供することを課題の一つとするものである。
○ Third problem Regarding the technology related to the replacement of egg yolk in foods, the technology of Patent Document 9 can provide the rich taste required for egg yolk substitute foods only by blending pumpkin yellow particles instead of egg yolk. It wasn't. In addition, in the technique of Patent Document 10, a flavor with a blue odor derived from soy protein used as a raw material may affect the flavor of the product, and egg white protein is required. May be affected by instability. Moreover, although the technology of Patent Document 11 attempts to reduce the unpleasant odor of soymilk by concentrating soymilk, it is not always satisfactory in flavor, and the quality of the mayonnaise-like food such as the richness is still not good. There was room for improvement. In the technique of Patent Document 12, the whey protein to be used must be highly purified by an ion exchange method adsorption resin, which is subject to manufacturing restrictions and is expensive. Therefore, an egg yolk substitute that is rich, flavorful and inexpensive to supply is desired.
Accordingly, an object of the present invention is to provide an egg yolk substitute composition that is rich and flavorful, is cheaper than egg yolk, and can be stably supplied, and a food using the same.
○第四の課題
 大豆加工食品に関する技術について、特許文献13,14はいずれも、まず一般的な製法で得た伝統的な豆腐を原料とし、これをすりつぶしてペースト状に加工したものを利用する技術である。これらは従来の豆腐をそのまま利用した加工品であるため、風味は豆腐そのものの風味でしかなく、食感も豆腐のゲルに由来するざらつきが感じられる場合がある。
 また、特許文献15の大豆蛋白質カードは豆腐様の食感をクリーム様にするために複雑な工程を要し、また豆乳を一旦酸沈殿させるために大豆の自然な風味とは異質な風味になってしまう。
 これに対して、本発明は、従来の豆腐等の大豆加工食品と差別化された、新たな風味と食感を有する大豆加工食品、あるいは従来品の風味と食感を向上させた大豆加工食品を提供することを課題の一つとする。
○ Fourth issue Regarding the technology related to processed soybean foods, Patent Documents 13 and 14 both use traditional tofu obtained by a general manufacturing method, ground and processed into a paste. Technology. Since these are processed products using conventional tofu as they are, the flavor is only the flavor of the tofu itself, and the texture may be felt from the tofu gel.
In addition, the soybean protein card of Patent Document 15 requires a complicated process to make the tofu-like texture cream, and the soy milk is acid-precipitated so that it has a different flavor from the natural flavor of soybean. End up.
On the other hand, the present invention is a soy processed food having a new flavor and texture that is differentiated from conventional processed soybean foods such as tofu, or a processed soybean food having improved flavor and texture of conventional products. Is one of the issues.
○第五の課題
 血清コレステロールの低減に関する技術について、本発明は、風味が改善された、血清コレステロール低減用組成物を課題の一つとする。また腎機能の改善に関する技術について、本発明は、風味が改善された、腎機能改善用組成物を課題の一つとする。
○ Fifth problem Regarding the technology relating to the reduction of serum cholesterol, the present invention has a composition for reducing serum cholesterol with improved flavor as one of the problems. Moreover, about the technique regarding the improvement of a renal function, this invention makes the composition for a renal function improvement by which flavor was improved one of the subjects.
 前記課題を解決する手段として、本発明は以下の解決手段を提供する。
(1)乾物あたりの蛋白質含量が25重量%以上であり脂質含量(クロロホルム/メタノール混合溶媒抽出物としての含量をいう。以下の項において同じ。)が蛋白質含量に対して100重量%以上でありかつLCI値が55%以上である大豆乳化組成物、又は、該大豆乳化組成物を含む原料を乳酸発酵もしくは酸添加により酸性化して得られる酸性大豆素材を含有することを特徴とする、大豆由来原料含有飲食品、
(2)乾物あたりの食物繊維含量が10重量%以下であって乾物あたりの脂質含量が35重量%以上である前記(1)記載の大豆乳化組成物、又は該大豆乳化組成物を含む原料を乳酸発酵もしくは酸添加により酸性化して得られる酸性大豆素材を含有することを特徴とする、大豆由来原料含有飲食品、
(3)前記(1)又は(2)記載の大豆乳化組成物又は酸性大豆素材を含むことを特徴とする、乳製品もしくは発酵乳製品代替物、
(4)前記(3)記載の乳製品もしくは発酵乳製品代替物が、乳製品もしくは発酵乳製品の一部又は全部の代替として使用されていることを特徴とする、大豆由来原料含有飲食品、
(5)前記(1)又は(2)記載の大豆乳化組成物を含む原料を、乳酸発酵又は酸添加により酸性化して得られることを特徴とする酸性大豆素材、
(6)前記(1)記載の大豆乳化組成物を含有することを特徴とする、卵黄代替組成物、
(7)前記(2)記載の大豆乳化組成物を含有することを特徴とする、卵黄代替組成物、
(8)前記(6)又は(7)記載の卵黄代替組成物が、卵黄の一部又は全部の代替として使用されていることを特徴とする卵黄代替食品、
(9)前記(1)記載の大豆乳化組成物が、大豆原料の一部又は全部として使用されていることを特徴とする大豆加工食品、
(10)前記(2)記載の大豆乳化組成物が、大豆原料の一部又は全部として使用されていることを特徴とする大豆加工食品、
(11)前記(1)記載の大豆乳化組成物を含有する、血清コレステロール低減用もしくは腎機能改善用組成物、
(12)前記(2)記載の大豆乳化組成物を含有する、血清コレステロール低減用もしくは腎機能改善用組成物、
(13)前記(11)又は(12)記載の血清コレステロール低減用もしくは腎機能改善用組成物を用いた食品、
(14)飲食品の製造において、前記(1)又は(2)記載の大豆乳化組成物を、乳製品、卵黄及び大豆原料から選択される原料の一部又は全部と代替して使用し、あるいは、血清コレステロール低減用もしくは腎機能改善用組成物として使用することを特徴とする、大豆由来原料含有飲食品の製造法、
(15)前記(1)又は(2)記載の大豆乳化組成物を、乳製品、卵黄及び大豆原料から選択される原料の一部もしくは全部と代替して使用し、あるいは、血清コレステロール低減用もしくは腎機能改善用組成物として使用することにより、大豆由来原料含有飲食品の風味又は食感を改良する方法。
 なお、上記(1)の大豆乳化組成物を以下、「本大豆乳化組成物」と称する。
As means for solving the above problems, the present invention provides the following means.
(1) The protein content per dry matter is 25% by weight or more and the lipid content (the content as a chloroform / methanol mixed solvent extract; the same applies in the following section) is 100% by weight or more with respect to the protein content. And a soybean emulsion composition having an LCI value of 55% or more, or an acidic soybean material obtained by acidifying a raw material containing the soybean emulsion composition by lactic acid fermentation or acid addition Raw material-containing foods and beverages,
(2) The soybean emulsified composition according to (1), wherein the dietary fiber content per dry matter is 10% by weight or less and the lipid content per dry matter is 35% by weight or more, or a raw material containing the soybean emulsified composition A soy-derived raw material-containing food or drink comprising an acidic soy material obtained by acidification by lactic acid fermentation or acid addition,
(3) A dairy product or fermented dairy product substitute comprising the soybean emulsified composition or acidic soybean material according to (1) or (2) above,
(4) The dairy product or fermented dairy product substitute described in (3) above is used as a substitute for part or all of the dairy product or fermented dairy product,
(5) An acidic soybean material obtained by acidifying a raw material containing the soybean emulsified composition according to (1) or (2) by lactic acid fermentation or acid addition,
(6) An egg yolk substitute composition comprising the soybean emulsified composition according to (1) above,
(7) An egg yolk substitute composition comprising the soybean emulsified composition according to (2),
(8) The egg yolk substitute food, wherein the egg yolk substitute composition according to (6) or (7) is used as a substitute for part or all of the egg yolk,
(9) The processed soybean food, wherein the soybean emulsified composition according to (1) is used as part or all of the soybean raw material,
(10) The processed soybean food, wherein the soybean emulsified composition according to (2) is used as part or all of the soybean raw material,
(11) A composition for reducing serum cholesterol or improving renal function, comprising the soybean emulsified composition according to (1),
(12) A composition for reducing serum cholesterol or improving renal function, comprising the soybean emulsified composition according to (2),
(13) A food using the composition for reducing serum cholesterol or improving renal function according to (11) or (12),
(14) In the production of food and drink, the soybean emulsified composition described in (1) or (2) above is used in place of part or all of a raw material selected from dairy products, egg yolk and soybean raw materials, or , A method for producing a food and drink containing soybean-derived materials, characterized by being used as a composition for reducing serum cholesterol or improving renal function,
(15) The soybean emulsified composition according to (1) or (2) above is used in place of part or all of a raw material selected from dairy products, egg yolk and soy raw materials, or for reducing serum cholesterol or A method for improving the flavor or texture of a soybean-derived material-containing food or drink by using it as a composition for improving renal function.
In addition, the soybean emulsified composition of the above (1) is hereinafter referred to as “the present soybean emulsified composition”.
 また本発明は、具体的な第一~第五の課題に即し、上記解決手段に包含される、より具体的な解決手段を以下提供する。 Further, the present invention provides the following more specific solution means included in the above solution means in accordance with specific first to fifth problems.
○本第一発明
 食品における乳製品の代替技術に関する第一の課題に鑑み、生クリームを代替しうるような風味の改善された大豆由来素材を得るために本発明者らは種々検討した結果、NSIが特定の範囲になるまで予め変性処理を施した加工大豆を用いて水抽出した不溶性画分が、中性脂質及び極性脂質が濃縮された大豆乳化組成物として得られ、本大豆乳化組成物が大豆由来の青臭みがなく濃厚なコク味のある大豆由来素材として有用であることを見出し、本第一発明を完成させた。
○ Invention of the present invention In view of the first problem related to dairy product replacement technology in foods, the present inventors have conducted various studies to obtain a soybean-derived material with improved flavor that can replace fresh cream, An insoluble fraction obtained by water extraction using processed soybean that has been modified in advance until the NSI reaches a specific range is obtained as a soybean emulsion composition in which neutral lipids and polar lipids are concentrated. Has been found to be useful as a soybean-derived material having a rich and rich taste without soybean-derived blue odor, and has completed the first invention.
 すなわち本第一発明は、下記(1)~(7)を含む発明を提供する。
(1)本大豆乳化組成物を含有することを特徴とする大豆由来原料含有飲食品、
(2)乾物あたりの食物繊維含量が10重量%以下である、前記(1)記載の大豆乳化組成物を含有することを特徴とする大豆由来原料含有飲食品、
(3)乾物あたりの脂質含量が15重量%以上であってNSIが20~77の範囲に加工された含脂大豆を用い、ア)該含脂大豆に加水して懸濁液を調製する工程、イ)該懸濁液を固液分離し、中性脂質及び極性脂質を不溶性画分に移行させて、蛋白質及び糖質を含む水溶性画分を除去し、不溶性画分を回収する工程、で得られる、前記(1)記載の大豆乳化組成物を含有することを特徴とする大豆由来原料含有飲食品、
(4)回収された不溶性画分をさらに均質化した後、該均質化液を固液分離して食物繊維を除去し、上清を回収して得られる、前記(3)記載の大豆乳化組成物を含有することを特徴とする大豆由来原料含有飲食品、
(5)風味改善された大豆由来原料含有飲食品への前記(1)~(4)いずれか記載の大豆乳化組成物の使用、
(6)前記(1)~(4)いずれか記載の大豆乳化組成物を、風味改善の有効成分として用いることを特徴とする、大豆由来原料含有飲食品の風味改善方法、
(7)前記(1)~(4)いずれか記載の大豆乳化組成物を、風味改善の有効成分として用いることを特徴とする、大豆由来原料含有飲食品の製造方法。
That is, the first invention provides the invention including the following (1) to (7).
(1) A soy-derived raw material-containing food or drink comprising the present soybean emulsified composition,
(2) A soybean-derived material-containing food or drink comprising the soybean emulsion composition according to (1), wherein the dietary fiber content per dry matter is 10% by weight or less,
(3) A step of preparing a suspension by using a fat-containing soybean whose lipid content per dry matter is 15% by weight or more and whose NSI is processed in the range of 20 to 77, and adding the oil to the fat-containing soybean. A) separating the suspension into solid and liquid, transferring neutral lipids and polar lipids to the insoluble fraction, removing the water-soluble fraction containing protein and carbohydrate, and collecting the insoluble fraction; A soybean-derived raw material-containing food or drink comprising the soybean emulsion composition according to (1), obtained by
(4) The soy emulsified composition according to (3), which is obtained by further homogenizing the recovered insoluble fraction and then separating the solidified liquid to remove dietary fiber and recovering the supernatant. Soy-derived raw material-containing food or drink characterized by containing foods,
(5) Use of the soybean emulsified composition according to any one of (1) to (4) above in a flavor-improved soybean-derived raw material-containing food or drink,
(6) A method for improving the flavor of food-containing foods containing soybean-derived materials, wherein the soybean emulsified composition according to any one of (1) to (4) is used as an active ingredient for flavor improvement,
(7) A method for producing a food / beverage product containing a soybean-derived material, wherein the soybean emulsified composition according to any one of (1) to (4) is used as an active ingredient for flavor improvement.
○本第二発明
 食品における発酵乳製品の代替技術に関する第二の課題に鑑み、本発明者らは、原料である大豆素材として豆乳や分離大豆蛋白を用い、種々検討を行ったが、従来の大豆素材を用いても乳のような濃厚なコクを有し、かつ好ましくない風味が抑えられた風味を出すことは、フレーバーを添加しなければ困難であった。また、滑らかな食感を得ることも澱粉や多糖類等の別の素材の添加なしには困難であった。
 そこで本発明者らはアプローチを変えてさらに鋭意検討を行う中、特定のユニークな組成を有する大豆素材を原料として使用することにより、濃厚なコクを有し、好ましくない風味も少なく、かつ滑らかな食感を有する酸性大豆素材を見出した。さらに、得られた酸性大豆素材を発酵乳の代わりに使用し、風味及び食感の良好な菓子・パン類を見出し、本第二発明を完成するに到った。
○ This second invention In view of the second problem regarding alternative technology of fermented milk products in foods, the present inventors have conducted various studies using soy milk and separated soy protein as a soy material as a raw material. Even if a soybean material is used, it has been difficult to produce a flavor that has a rich body like milk and has an unfavorable flavor suppressed. In addition, it is difficult to obtain a smooth texture without the addition of other materials such as starch and polysaccharides.
Therefore, the present inventors have changed their approach and are eagerly studying so that by using a soybean material having a specific unique composition as a raw material, it has a rich body, less unfavorable flavor, and smooth. An acidic soybean material having a texture was found. Furthermore, the obtained acidic soybean material was used in place of fermented milk, and confectionery and bread having a good flavor and texture were found, and the present second invention was completed.
 すなわち本第二発明は、下記(1)~(10)を含む発明を提供する。
(1)本大豆乳化組成物を含む原料を乳酸発酵又は酸添加により酸性化して得られることを特徴とする酸性大豆素材、
(2)酸性大豆素材がチーズタイプ又はヨーグルトタイプである、前記(1)記載の素材、
(3)酸性大豆素材が、酸性化後にホエーを分離しカードを回収して得られる、ホエー分離タイプである、前記(1)記載の素材、
(4)酸性大豆素材が、酸性化後にホエーを分離しない、ホエー非分離タイプである、前記(1)記載の素材、
(5)原料を酸性化した後にリン酸塩を添加する、ホエー分離タイプ又はホエー非分離タイプの前記(1)記載の素材、
(6)前記(1)記載の酸性大豆素材を使用した食品、
(7)酸性大豆素材が主原料、又は、練り込み用、フィリング用、トッピング用もしくはナッペ用の副原料として使用されている、前記(2)記載の食品、
(8)前記(1)記載の酸性大豆素材を主原料として使用した、レアチーズケーキ様食品、
(9)前記(1)記載の酸性大豆素材を主原料として使用した、ベイクドチーズケーキ様食品、 
(10)本大豆乳化組成物を含む原料を乳酸発酵又は酸添加により酸性化することを特徴とする酸性大豆素材の製造法。
That is, the second invention provides the invention including the following (1) to (10).
(1) An acidic soybean material obtained by acidifying a raw material containing the present soybean emulsified composition by lactic acid fermentation or acid addition,
(2) The material according to (1), wherein the acidic soybean material is a cheese type or a yogurt type,
(3) The material according to (1), wherein the acidic soybean material is a whey separation type obtained by separating the whey after acidification and collecting the card.
(4) The material according to (1), wherein the acidic soybean material is a whey non-separable type that does not separate whey after acidification,
(5) The material according to the above (1) of the whey separation type or the whey non-separation type, in which the phosphate is added after acidifying the raw material,
(6) A food using the acidic soybean material according to (1),
(7) The food according to (2) above, wherein the acidic soybean material is used as a main ingredient, or as an auxiliary ingredient for kneading, filling, topping or nappe,
(8) A rare cheesecake-like food using the acidic soybean material according to (1) as a main ingredient,
(9) A baked cheesecake-like food using the acidic soybean material according to (1) as a main ingredient,
(10) A method for producing an acidic soybean material, characterized by acidifying a raw material containing the present emulsion emulsified composition by lactic acid fermentation or acid addition.
○本第三発明
 食品における卵黄の代替技術に関する第三の課題に鑑み、本発明者らは鋭意研究を行った結果、本大豆乳化組成物がコクがあって風味の良い卵黄代替組成物として、調味料、菓子類、ソース類、惣菜等の食品に使用できるという知見を得、本第三発明を完成した。
○ This third invention In view of the third problem related to egg yolk substitute technology in food, the present inventors have conducted intensive research, and as a result, the soybean emulsion composition has a rich and delicious egg yolk substitute composition. Obtaining the knowledge that it can be used in foods such as seasonings, confectionery, sauces, side dishes, etc., the present third invention has been completed.
 すなわち本第三発明は、下記(1)~(9)を含む発明を提供する。
(1)本大豆乳化組成物を含有することを特徴とする、卵黄代替組成物、
(2)さらに糖類、デンプン、香辛料、油脂、酸化防止剤及び凝固剤の群から選ばれる1種以上が添加されている、前記(1)記載の卵黄代替組成物、
(3)さらに蛋白質架橋酵素を作用させたものである、前記(1)又は(2)記載の卵黄代替組成物、
(4)前記(1)~(3)何れか記載の卵黄代替組成物を使用した卵黄代替食品、
(5)卵黄代替食品が調味料、菓子類、ソース類又は惣菜である、前記(4)記載の卵黄代替食品、
(6)調味料がマヨネーズ、ドレッシング類、たれ類又は乾燥調味料である、前記(5)記載の卵黄代替食品、
(7)菓子類が、クリーム類、デザート類又は焼き菓子である、前記(5)記載の卵黄代替食品、
(8)本大豆乳化組成物の卵黄代替組成物としての使用、
(9)本大豆乳化組成物を卵黄原料の一部又は全部の代替として使用することを特徴とする、卵黄代替食品の製造方法。
That is, the third invention provides inventions including the following (1) to (9).
(1) An egg yolk substitute composition comprising the present soybean emulsified composition,
(2) The egg yolk substitute composition according to (1), wherein one or more selected from the group of sugars, starches, spices, fats and oils, antioxidants and coagulants are added,
(3) The egg yolk substitute composition according to the above (1) or (2), wherein a protein cross-linking enzyme is further acted on,
(4) An egg yolk substitute food using the egg yolk substitute composition according to any one of (1) to (3),
(5) The egg yolk substitute food according to the above (4), wherein the egg yolk substitute food is a seasoning, confectionery, sauces or sugar beet,
(6) The egg yolk substitute food according to (5) above, wherein the seasoning is mayonnaise, dressings, sauces or dry seasonings,
(7) The egg yolk substitute food according to (5), wherein the confectionery is a cream, a dessert or a baked confectionery,
(8) Use of the present soybean emulsified composition as an egg yolk substitute composition,
(9) A method for producing a yolk substitute food, characterized in that the soybean emulsion composition is used as a substitute for a part or all of a yolk raw material.
○本第四発明
 大豆加工食品の技術に関する第四の課題に鑑み、本発明者らは種々検討した結果、NSIが特定の範囲になるまで予め変性処理を施した加工大豆を用いて水抽出した不溶性画分から、中性脂質及び極性脂質が濃縮された大豆乳化組成物として得られ、本大豆乳化組成物が大豆由来の青臭みがなく濃厚なコク味のある大豆由来素材として有用であることを見出した。そして、これを豆乳の一部又は全部に代替して大豆加工食品の原料として使用したところ、従来の豆乳とは異なる物性を示し、食感や風味に特徴のある大豆加工食品を得るに到り、本第四発明を完成させた。
○ This Fourth Invention In view of the fourth problem related to the technology of processed soybean foods, the present inventors have conducted various studies, and as a result, water extraction was performed using processed soybeans that had been modified in advance until the NSI reached a specific range. It is obtained from the insoluble fraction as a soy emulsified composition in which neutral lipids and polar lipids are concentrated, and the soy emulsified composition is useful as a soy-derived material having a rich and rich taste without the blue odor derived from soy. I found it. And when this was replaced with a part or all of soy milk and used as a raw material for processed soybean food, it resulted in obtaining a processed soybean food having physical properties different from those of conventional soy milk and characterized by texture and flavor. The fourth invention has been completed.
 すなわち本第四発明は、下記(1)~(7)を含む発明を提供する。
(1)本大豆乳化組成物が、原料として使用されていることを特徴とする大豆加工食品、
(2)該大豆乳化組成物中の脂質含量が乾物あたり35重量%以上である、前記(1)記載の大豆加工食品、
(3)該大豆乳化組成物中の食物繊維含量が乾物あたり10重量%以下である、前記(1)記載の大豆加工食品。
(4)大豆乳化組成物中における全蛋白質あたりのリポキシゲナーゼ蛋白質の含量が4%以上である、前記(1)記載の大豆加工食品、
(5)大豆加工食品が、豆腐類、湯葉、大豆調味料、油揚げ、がんもどき又は厚揚げである、前記(1)記載の大豆加工食品、
(6)本大豆乳化組成物を含有することを特徴とする、大豆加工食品製造用の食感又は風味の改良剤、
(7)本大豆乳化組成物を原料に使用することを特徴とする、大豆加工食品の食感又は風味の改良方法。
That is, the fourth invention provides inventions including the following (1) to (7).
(1) The soybean processed food, wherein the soybean emulsion composition is used as a raw material,
(2) The processed soybean food according to (1), wherein the soybean emulsion composition has a lipid content of 35% by weight or more per dry matter,
(3) The processed soybean food according to (1) above, wherein the dietary fiber content in the soybean emulsified composition is 10% by weight or less per dry matter.
(4) The processed soybean food according to (1), wherein the content of lipoxygenase protein per total protein in the soybean emulsified composition is 4% or more,
(5) The processed soybean food according to the above (1), wherein the processed soybean food is tofu, yuba, soybean seasoning, deep-fried oil, cancer throb or deep-fried food,
(6) A texture or flavor improver for the production of processed soybean food, comprising the present soybean emulsion composition,
(7) A method for improving the texture or flavor of processed soybean food, characterized by using the present soybean emulsified composition as a raw material.
○本第五発明
 血清コレステロールの低減技術又は腎機能の改善技術に関する第五の課題に鑑み、本発明者らは鋭意研究を行った。
 特許文献16においては、血清コレステロール低減用組成物の作用中心が11Sグロブリンであるとし、これを多く含有するように分画する旨記載されている。しかし、特許文献16においては、11Sグロブリンを多く含有する画分は、脱脂大豆を出発原料として調製されているため、脱脂大豆に由来する風味が含まれる場合がある。
 特許文献17においては、血中コレステロール低下粉末素材について記載されている。しかしこれも脱脂大豆を原料とするものでかつ、組織化処理することが必須の構成要件となっていることから、脱脂大豆に由来する風味が含まれる場合があることに加え、調製が煩雑である。
 特許文献18においても、具体的開示は脱脂大豆を原料として用いるものであり、得られたものは脱脂大豆に由来する風味が含まれる場合がある。
 また引用文献19には「非7S・11S-酸沈殿性大豆蛋白」に強い尿中アルブミン低下作用がある」との記載がある。しかし当該「非7S・11S-酸沈殿性大豆蛋白」は脱脂大豆を出発原料として調製されているため、脱脂大豆に由来する風味が含まれる場合がある。
○ Fifth Invention In view of the fifth problem related to the technology for reducing serum cholesterol or the technology for improving renal function, the present inventors have conducted intensive research.
In Patent Document 16, it is described that the action center of the composition for reducing serum cholesterol is 11S globulin, and fractionation is performed so as to contain a large amount thereof. However, in Patent Document 16, since a fraction containing a large amount of 11S globulin is prepared using defatted soybean as a starting material, a flavor derived from defatted soybean may be included.
Patent Document 17 describes a blood cholesterol-lowering powder material. However, this also uses defatted soybean as a raw material, and since it is an essential constituent requirement to perform an organized treatment, in addition to the fact that flavors derived from defatted soybean may be included, preparation is complicated. is there.
Also in Patent Document 18, the specific disclosure uses defatted soybean as a raw material, and the obtained product may contain a flavor derived from defatted soybean.
Reference 19 describes that “non-7S / 11S-acid-precipitating soybean protein has a strong urinary albumin lowering effect”. However, since the “non-7S · 11S-acid-precipitating soybean protein” is prepared using defatted soybean as a starting material, a flavor derived from defatted soybean may be included.
 上記のような状況に鑑み、本発明者はさらに研究を重ねた結果、脱脂大豆を出発原料とすることなく、特定の方法で調製した大豆由来乳化組成物は、風味も極めて良好で、かつ、強い血清コレステロール低減作用及び腎機能改善作用を示すことを見出し、本第五発明を完成させた。 In view of the situation as described above, as a result of further research, the inventor-derived emulsion composition prepared by a specific method without using defatted soybean as a starting material has a very good flavor, and It was found that it exhibits a strong serum cholesterol reducing action and a renal function improving action, and the fifth invention has been completed.
 すなわち本第五発明は、下記(1)~(5)を含む発明を提供する。
(1)本大豆乳化組成物を含有する、血清コレステロール低減用もしくは腎機能改善用組成物、
(2)脂質が未精製大豆油である、前記(1)記載の大豆乳化組成物を含有する血清コレステロール低減用もしくは腎機能改善用組成物、
(3)乾物あたりの脂質含量が15重量%以上であってNSIが20~77の範囲に加工された含脂大豆を用い、ア)該含脂大豆に加水して懸濁液を調製する工程、イ)該懸濁液を固液分離し、中性脂質及び極性脂質を不溶画分に移行させて、蛋白質及び糖質を含む水溶性画分を除去し、不溶性画分を回収する工程、で得られる、前記(1)又は(2)記載の大豆乳化組成物を含有する血清コレステロール低減用もしくは腎機能改善用組成物、
(4)前記(1)記載の血清コレステロール低減用もしくは腎機能改善用組成物を用いた食品、
(5)前記(3)記載の、血清コレステロール低減用もしくは腎機能改善用組成物を用いた食品の製造法。
That is, the fifth invention provides the invention including the following (1) to (5).
(1) A composition for reducing serum cholesterol or improving renal function, comprising the present soybean emulsified composition,
(2) A composition for reducing serum cholesterol or improving renal function, comprising the soybean emulsion composition according to (1), wherein the lipid is unrefined soybean oil;
(3) A step of preparing a suspension by using a fat-containing soybean having a lipid content per dry matter of 15% by weight or more and having an NSI processed in the range of 20 to 77, a) adding to the fat-containing soybean A) solid-liquid separation of the suspension, transferring neutral lipids and polar lipids to the insoluble fraction, removing the water-soluble fraction containing proteins and carbohydrates, and collecting the insoluble fraction; A composition for reducing serum cholesterol or improving renal function, comprising the soybean emulsified composition according to (1) or (2), obtained by
(4) A food using the composition for reducing serum cholesterol or improving renal function according to (1),
(5) A method for producing a food using the composition for reducing serum cholesterol or improving renal function according to (3) above.
 本第一発明の、含脂大豆から得られる中性脂質及び極性脂質が濃縮された本大豆乳化組成物は大豆特有の青臭みがなく、濃厚なコク味、自然な大豆の旨味に優れた風味を有し、本大豆乳化組成物を乳製品の代替物として用いることにより、風味が大幅に改善された大豆由来原料含有飲食品を提供することができる。 The present soybean emulsified composition enriched with neutral lipids and polar lipids obtained from fat-containing soybeans according to the first invention has no blue smell peculiar to soybeans, a rich body taste, and a flavor excellent in natural soybean taste By using the present soybean emulsified composition as a substitute for dairy products, it is possible to provide a soy-derived raw material-containing food or drink that has a significantly improved flavor.
 本第二発明によれば、従来の豆乳や分離大豆蛋白を乳酸発酵させたときに感ずる劣化臭や酢酸臭・収斂味といった好ましくない風味が非常に少ない酸性大豆素材を提供することができる。
 そして本酸性大豆素材は、チーズやヨーグルトが利用されている各種食品へ使用することができ、チーズやヨーグルトの一部又は全部の代替が可能である。
 一例として、洋生菓子においてはチーズの代替として本酸性大豆素材をレアチーズケーキの主原料に用いれば、チーズで作製したものと遜色ないものにすることも可能であり、コクがあり、且つ大豆由来の爽やかさのある滑らかな食感のレアチーズケーキ様食品を得ることができる。
 また、ベイクドチーズケーキの主原料に用いても、チーズで作製したものと外観上遜色なく、かつコクのある風味を有すると共に、食感についてはホクホクとした芋のような食感を有し、通常のベイクドチーズケーキとは別の特徴を有する新しいベイクドチーズケーキ様食品を得ることができる。
According to the second aspect of the present invention, it is possible to provide an acidic soy material that has very few undesirable flavors such as a deteriorated odor, an acetic acid odor, and an astringent taste that are felt when lactic fermentation of conventional soy milk or isolated soy protein.
And this acidic soybean raw material can be used for the various foodstuffs in which cheese and yoghurt are utilized, and a part or all substitution of cheese and yoghurt is possible.
As an example, in Western confectionery, if this acidic soy material is used as the main raw material for rare cheesecake as an alternative to cheese, it can be made inferior to that made with cheese, is rich, and is derived from soybeans. A rare cheesecake-like food with a refreshing and smooth texture can be obtained.
In addition, even when used as the main raw material of baked cheesecake, it has a flavor that is not inferior in appearance and rich in appearance with cheese, and has a texture like a crunchy rice cake, A new baked cheesecake-like food product having characteristics different from that of a normal baked cheesecake can be obtained.
 本第三発明によれば、本大豆乳化組成物を含有する卵黄代替組成物を使用することにより、クリーミーでコクがあり、風味が良好な卵黄代替食品を製造することができる。 According to the third invention, by using the egg yolk substitute composition containing the present soybean emulsified composition, an egg yolk substitute food that is creamy and rich and has a good flavor can be produced.
 本第四発明によれば、本大豆乳化組成物を各種大豆加工食品の原料として用いることにより、概して大豆特有の青臭みがなく、濃厚なコク味と自然な大豆の旨味を付与し、またクリーミーな食感を付与することができ、風味や食感において従来品と差別化された特徴のある大豆加工食品を提供することができる。
 特に豆腐類や厚揚げの場合は、軟らかく、従来の製品よりもクリーミーで、口の中でとろけるような口溶けの良い食感のものが得られる。
 また湯葉の場合は、容易にすくいたての生湯葉(汲み上げ湯葉)のようなとろりとした食感のものが得られる。
 またがんもどきや油揚げのような膨化食品は、従来の製品よりもフライ調理した際の生地原料の膨化がより促進され、膨化率を向上させる効果を与えることができる。そして膨化率が向上することにより、調理後の調味液の吸収力も向上し、よりジューシーな食感を与えることができる。
According to the fourth aspect of the present invention, the soy emulsion composition is used as a raw material for various processed soybean foods, so that generally there is no blue smell characteristic of soybeans, a rich body taste and a natural soybean taste are imparted, and creamy A processed soy food with characteristics that are differentiated from conventional products in flavor and texture can be provided.
In particular, in the case of tofu and deep-fried food, it is softer, creamier than conventional products, and has a mouthfeel that melts in the mouth.
In the case of Yuba, it is easy to obtain a thick texture like freshly-cured Yuba (pumped Yuba).
In addition, puffed foods such as cancer pounding and deep-fried food can promote the expansion of dough raw materials when fried and cooked more than conventional products, and can give the effect of improving the puffing rate. And the expansion rate improves, The absorptive power of the seasoning liquid after cooking can also improve, and a more juicy food texture can be given.
 本第五発明による血清コレステロール低減用もしくは腎機能改善用組成物は、良好な風味と血清コレステロール低減作用もしくは腎機能改善作用を有するものであり、これを用いることで、コレステロール低減効果又は腎機能改善効果を示す、風味の優れた各種食品を容易に得ることができる。 The composition for reducing serum cholesterol or improving renal function according to the fifth invention has a good flavor and serum cholesterol reducing action or renal function improving action. By using this composition, the cholesterol reducing effect or renal function improving action is obtained. Various foods with excellent flavor and effects can be easily obtained.
ラットの2週間摂食時の血清コレステロール濃度を示す図である。It is a figure which shows the serum cholesterol density | concentration at the time of 2-week feeding of a rat. ラットの2週間摂食時の尿中NAG活性を示す図である。It is a figure which shows the urinary NAG activity at the time of 2-week feeding of a rat.
 本第一発明の大豆由来原料含有飲食品、本第三発明の卵黄代替組成物、本第四発明の大豆加工食品、本第五発明の血清コレステロール低減用もしくは腎機能改善用組成物は、何れも共通して下記に説明する新規な「大豆乳化組成物」を含有することが特徴である。また本第二発明の酸性大豆素材も、該大豆乳化組成物を含む原料を乳酸発酵又は酸添加により酸性化して得られることが特徴である。詳しくは、日本国出願(特願2011-108598号)の明細書に記載される内容を援用するが、以下、本第一~第五発明において共通する特別な技術的特徴である、本大豆乳化組成物について説明する。 The soybean-derived raw material-containing food and drink of the first invention, the egg yolk substitute composition of the third invention, the processed soybean food of the fourth invention, and the composition for reducing serum cholesterol or improving renal function of the fifth invention are any Is also characterized by containing a novel “soybean emulsified composition” described below. The acidic soybean material of the second invention is also characterized by being obtained by acidifying a raw material containing the soybean emulsified composition by lactic acid fermentation or acid addition. For details, the contents described in the specification of the Japanese application (Japanese Patent Application No. 2011-108598) are incorporated, but hereinafter, the present soybean emulsification, which is a special technical feature common to the first to fifth inventions. The composition will be described.
<大豆乳化組成物>
 本発明に用いられる大豆乳化組成物は、大豆を由来とし、蛋白質のうち、グリシニンやβ-コングリシニン以外の脂質親和性蛋白質(あるいは別の指標としてリポキシゲナーゼ蛋白質)の割合が特に高く、中性脂質及び極性脂質を多く含む乳化組成物である。すなわち、乾物あたりの蛋白質含量が25重量%以上、乾物あたりの脂質含量(クロロホルム/メタノール混合溶媒抽出物としての含量をいう。)が乾物あたりの蛋白質含量に対して100重量%以上であって、LCI値(Lipophilic Proteins Content Index)が55%以上、より好ましくは60%以上であることを主要な特徴とするものである。
<Soy emulsified composition>
The soybean emulsified composition used in the present invention is derived from soybean, and has a particularly high ratio of lipophilic proteins other than glycinin and β-conglycinin (or lipoxygenase protein as another index) among proteins, neutral lipids and It is an emulsified composition containing a large amount of polar lipids. That is, the protein content per dry matter is 25% by weight or more, and the lipid content per dry matter (referred to as a chloroform / methanol mixed solvent extract) is 100% by weight or more with respect to the protein content per dry matter, The main characteristic is that the LCI value (Lipophilic Proteins Content Index) is 55% or more, more preferably 60% or more.
(脂質)
 一般に脂質含量はエーテル抽出法で測定されるが、本大豆乳化組成物中には中性脂質の他にエーテルで抽出されにくい極性脂質も多く含まれるため、本発明における脂質含量は、クロロホルム:メタノールが2:1(体積比)の混合溶媒を用い、常圧沸点において30分間抽出された抽出物量を総脂質量として、脂質含量を算出した値とする。溶媒抽出装置としてはFOSS社製の「ソックステック」を用いることができる。なお上記の測定法は「クロロホルム/メタノール混合溶媒抽出法」と称するものとする。
(Lipid)
In general, the lipid content is measured by an ether extraction method, but since the soybean emulsion composition contains a lot of polar lipids that are difficult to extract with ether in addition to neutral lipids, the lipid content in the present invention is chloroform: methanol. Is a value obtained by calculating the lipid content using a mixed solvent of 2: 1 (volume ratio) and the amount of extract extracted for 30 minutes at the normal pressure boiling point as the total amount of lipid. As the solvent extraction device, “Soxtec” manufactured by FOSS can be used. The above measurement method is referred to as “chloroform / methanol mixed solvent extraction method”.
 本大豆乳化組成物は、この大豆粉の脂質含量/蛋白質含量の比よりも高い値の脂質を含み、特に極性脂質に富むことが特徴である。該脂質は原料となる大豆に由来する脂質である。 The present soybean emulsified composition contains a lipid having a value higher than the ratio of the lipid content / protein content of the soy flour, and is characterized by being particularly rich in polar lipids. The lipid is a lipid derived from soybean as a raw material.
 本大豆乳化組成物の脂質含量は、乾物あたりの蛋白質含量に対して100重量%以上、好ましくは120~250重量%、さらに好ましくは120~200重量%であり、蛋白質よりも脂質が多いことが特徴である。また構成に必須ではないが、脂質含量を絶対量で表す場合、乾物あたり35重量%以上、好ましくは40重量%以上であるのが適当である。本大豆乳化組成物を繊維質等が除去されたものとすれば脂質含量を乾物あたり50重量%以上にもすることができる。また脂質含量の上限は限定されないが、好ましくは75重量%以下、より好ましくは70重量%以下である。 The lipid content of the present soybean emulsified composition is at least 100% by weight, preferably 120 to 250% by weight, more preferably 120 to 200% by weight, based on the protein content per dry matter. It is a feature. Although not essential for the constitution, when the lipid content is expressed in absolute amount, it is appropriate that it is 35% by weight or more, preferably 40% by weight or more per dry matter. If the soybean emulsified composition is one in which fibers and the like are removed, the lipid content can be 50% by weight or more per dry matter. The upper limit of the lipid content is not limited, but is preferably 75% by weight or less, more preferably 70% by weight or less.
(蛋白質)
 本大豆乳化組成物の蛋白質含量は乾物あたり25重量%以上、好ましくは30重量%以上である。また蛋白質含量の上限は限定されないが、好ましくは50重量%以下、より好ましくは40重量%以下である。
(protein)
The protein content of the present soybean emulsified composition is 25% by weight or more, preferably 30% by weight or more per dry matter. The upper limit of the protein content is not limited, but is preferably 50% by weight or less, more preferably 40% by weight or less.
○蛋白質含量の分析
 本発明における蛋白質含量はケルダール法により窒素量として測定し、該窒素量に6.25の窒素換算係数を乗じて求めるものとする。
Analysis of protein content The protein content in the present invention is measured as the amount of nitrogen by the Kjeldahl method, and is obtained by multiplying the amount of nitrogen by a nitrogen conversion factor of 6.25.
○蛋白質の各成分の組成分析
 本大豆乳化組成物の蛋白質の各成分組成はSDSポリアクリルアミドゲル電気泳動(SDS-PAGE)により分析することができる。
 界面活性剤であるSDSと還元剤であるメルカプトエタノールの作用によって蛋白質分子間の疎水性相互作用、水素結合、分子間のジスルフィド結合が切断され、マイナスに帯電した蛋白質分子は固有の分子量に従った電気泳動距離を示ことにより、蛋白質に特徴的な泳動パターンを呈する。電気泳動後に色素であるクマシーブリリアントブルー(CBB)にてSDSゲルを染色した後に、デンシトメーターを用い、全蛋白質のバンドの濃さに対する各種蛋白質分子に相当するバンドの濃さが占める割合を算出する方法により求めることができる。
-Composition analysis of each component of protein The component composition of the protein of this soybean emulsified composition can be analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE).
Hydrophobic interactions, hydrogen bonds, and intermolecular disulfide bonds between protein molecules are cleaved by the action of the surfactant SDS and the reducing agent mercaptoethanol, and negatively charged protein molecules follow their intrinsic molecular weight. By showing the electrophoretic distance, it exhibits a migration pattern characteristic of proteins. After electrophoresis, after staining the SDS gel with Coomassie Brilliant Blue (CBB), calculate the ratio of the density of bands corresponding to various protein molecules to the density of all protein bands using a densitometer. It can be obtained by the method to do.
(リポキシゲナーゼ蛋白質)
 本大豆乳化組成物は、一般に大豆中のオイルボディにはほとんど含まれないリポキシゲナーゼ蛋白質が特定量以上含まれることが大きな特徴であり、本大豆乳化組成物中の全蛋白質あたり少なくとも4%以上含有し、好ましくは5%以上含有するものである。
 通常の未変性(NSI 90以上)の大豆を原料とした場合ではリポキシゲナーゼ蛋白質は可溶性の状態で存在するため、水抽出すると水溶性画分側へ抽出される。一方、本発明ではリポキシゲナーゼ蛋白質が原料大豆中において加熱処理によって失活され不溶化しているため、不溶性画分側に残る。
 蛋白質中におけるリポキシゲナーゼ蛋白質の割合が高まることによって油脂の乳化状態が安定化されるばかりでなく、グロブリン蛋白質を主体とした通常の大豆蛋白質組成では得られない滑らかな物性の食感を得ることができ、また素材にコクのある風味が付与される。
(Lipoxygenase protein)
This soybean emulsified composition is characterized in that it contains a specific amount or more of lipoxygenase protein that is generally not contained in the oil body in soybean, and contains at least 4% or more per total protein in the soybean emulsion composition. , Preferably 5% or more.
When normal undenatured (NSI 90 or higher) soybeans are used as the raw material, the lipoxygenase protein exists in a soluble state, and therefore when extracted with water, it is extracted to the water-soluble fraction side. On the other hand, in the present invention, the lipoxygenase protein remains in the insoluble fraction side because it is inactivated and insolubilized in the raw soybean by heat treatment.
The increase in the proportion of lipoxygenase protein in the protein not only stabilizes the emulsified state of fats and oils, but also provides a smooth texture that cannot be obtained with a normal soy protein composition mainly composed of globulin proteins. In addition, a rich flavor is added to the material.
 リポキシゲナーゼ蛋白質の場合は通常L-1、L-2、L-3の3種類が存在し、上記の電気泳動法により、リポキシゲナーゼ蛋白質に相当するこれらのバンドの濃さから含量を算出できる。 In the case of lipoxygenase protein, there are usually three types, L-1, L-2 and L-3, and the content can be calculated from the intensity of these bands corresponding to the lipoxygenase protein by the above-mentioned electrophoresis method.
(脂質親和性蛋白質)
 本大豆乳化組成物は、蛋白質の種類の中では脂質親和性蛋白質(Lipophilic Proteins)が一般の大豆素材より多く含まれることが特徴である。脂質親和性蛋白質は、大豆の主要な酸沈殿性大豆蛋白質の内、グリシニン(7Sグロブリン)とβ-コングリシニン(11Sグロブリン)以外のマイナーな酸沈殿性大豆蛋白質群をいい、レシチンや糖脂質などの極性脂質を多く随伴するものである。以下、単に「LP」と略記することがある。
 LPは雑多な蛋白質が混在したものであるが故、各々の蛋白質を全て特定し、LPの含量を厳密に測定することは困難であるが、下記LCI(Lipophilic Proteins Content Index)値を求めることにより推定することができる。これによれば、本大豆乳化組成物中の蛋白質のLCI値は通常55%以上であり、好ましくは58%以上、より好ましくは60%以上であり、さらに好ましくは63%以上、最も好ましくは65%以上である。
 通常の未変性(NSI 90以上)の大豆を原料とした場合ではLPは可溶性の状態で存在するため、水抽出すると水溶性画分側へ抽出される。一方、本大豆乳化組成物の場合、LPが原料大豆中において加熱処理によって失活され不溶化しているため、不溶性画分側に残る。
 蛋白質中におけるLPの割合が高まることによって油脂の乳化状態が安定化されるばかりでなく、グロブリン蛋白質を主体とした通常の大豆蛋白質組成では得られない滑らかな物性の食感を得ることができ、また素材にコクのある風味が付与される。
(Lipophilic protein)
The present soybean emulsified composition is characterized in that among the types of proteins, lipophilic proteins are contained more than general soybean materials. Lipophilic protein refers to a group of minor acid-precipitating soybean proteins other than glycinin (7S globulin) and β-conglycinin (11S globulin) among the major acid-precipitating soybean proteins of soybean, such as lecithin and glycolipids. It accompanies many polar lipids. Hereinafter, it may be simply abbreviated as “LP”.
LP is a mixture of miscellaneous proteins, so it is difficult to identify each protein and measure the LP content precisely, but by calculating the following LCI (Lipophilic Proteins Content Index) value Can be estimated. According to this, the LCI value of the protein in the present soybean emulsified composition is usually 55% or more, preferably 58% or more, more preferably 60% or more, further preferably 63% or more, and most preferably 65%. % Or more.
When normal undenatured soybeans (NSI 90 or higher) are used as raw materials, LP exists in a soluble state, so when extracted with water, it is extracted to the water-soluble fraction. On the other hand, in the case of the present soybean emulsified composition, LP remains in the insoluble fraction side because LP is inactivated and insolubilized by heat treatment in the raw soybean.
The increase in the proportion of LP in the protein not only stabilizes the emulsified state of the fats and oils, but also provides a smooth texture that cannot be obtained with a normal soy protein composition mainly composed of globulin proteins. In addition, a rich flavor is imparted to the material.
○LP含量の推定・LCI値の測定方法
(a) 各蛋白質中の主要な蛋白質として、7Sはαサブユニット及びα'サブユニット(α+α')、11Sは酸性サブユニット(AS)、LPは34kDa蛋白質及びリポキシゲナーゼ蛋白質(P34+Lx)を選択し、SDS-PAGEにより選択された各蛋白質の染色比率を求める。電気泳動は表1の条件で行うことが出来る。
(b) X(%)=(P34+Lx)/{(P34+Lx)+(α+α’)+AS}×100(%)を求める。
(c) 低変性脱脂大豆から調製された分離大豆蛋白のLP含量を加熱殺菌前に上記方法1,2の分画法により測定すると凡そ38%となることから、X=38(%)となるよう(P34+Lx)に補正係数k*=6を掛ける。
(d) すなわち、以下の式によりLP推定含量(Lipophilic Proteins Content Index、以下「LCI」と略する。)を算出する。
○ LP content estimation / LCI value measurement method
(a) As the main protein in each protein, 7S selects α subunit and α ′ subunit (α + α ′), 11S selects acidic subunit (AS), LP selects 34 kDa protein and lipoxygenase protein (P34 + Lx), Obtain the staining ratio of each protein selected by SDS-PAGE. Electrophoresis can be performed under the conditions shown in Table 1.
(b) X (%) = (P34 + Lx) / {(P34 + Lx) + (α + α ′) + AS} × 100 (%) is obtained.
(c) Since the LP content of the separated soybean protein prepared from low-denatured defatted soybean is measured by the fractionation method of methods 1 and 2 before heat sterilization, it is about 38%, so X = 38 (%). (P34 + Lx) is multiplied by the correction coefficient k * = 6.
(d) That is, the estimated LP content (Lipophilic Proteins Content Index, hereinafter abbreviated as “LCI”) is calculated by the following equation.
(表1)
Figure JPOXMLDOC01-appb-I000001
Figure JPOXMLDOC01-appb-I000002
(Table 1)
Figure JPOXMLDOC01-appb-I000001
Figure JPOXMLDOC01-appb-I000002
(乾物含量)
 本大豆乳化組成物は通常生クリーム様の性状であり、通常の乾物(dry matter)は20~30重量%程度であるが、特に限定されるものではない。すなわち加水により低粘度の液状としたものや、濃縮加工されてより高粘度のクリーム状としたものであってもよく、また粉末加工されて粉末状としたものであってもよい。
(Dry matter content)
The soybean emulsified composition usually has a fresh cream-like property, and the usual dry matter is about 20 to 30% by weight, but is not particularly limited. That is, it may be a liquid having a low viscosity by water addition, a cream having a higher viscosity by concentration, or a powder processed to be a powder.
(大豆乳化組成物の製造態様)
 本大豆乳化組成物は、例えば水溶性窒素指数(Nitrogen Solubility Index、以下「NSI」と称する。)が20~77、好ましくは20~70、乾物あたりの脂質含量が15重量%以上の全脂大豆などの含脂大豆に対して、加水して懸濁液を調製する工程の後、該懸濁液を固液分離し、中性脂質及び極性脂質を不溶性画分に移行させて、蛋白質及び糖質を含む水溶性画分を除去し、不溶性画分を回収することにより得ることができる。以下、該製造態様について示す。
(Manufacturing aspect of soybean emulsified composition)
The soybean emulsified composition has a water-soluble nitrogen index (Nitrogen Solubility Index, hereinafter referred to as “NSI”) of 20 to 77, preferably 20 to 70, and a full fat soybean having a lipid content of 15% by weight or more per dry matter. After the step of preparing a suspension by adding water to a fat-containing soybean, etc., the suspension is subjected to solid-liquid separation, neutral lipids and polar lipids are transferred to an insoluble fraction, and protein and sugar It can be obtained by removing the water-soluble fraction containing the quality and collecting the insoluble fraction. Hereinafter, this production mode will be described.
○原料大豆及びその加工
 本大豆乳化組成物の原料である大豆としては、全脂大豆あるいは部分脱脂大豆等の含脂大豆を用いる。部分脱脂大豆としては、全脂大豆を圧搾抽出等の物理的な抽出処理により部分的に脱脂したものが挙げられる。一般に全脂大豆中には脂質が乾物あたり約20~30重量%程度含まれ、特殊な大豆品種については脂質が30重量%以上のものもあり、特に限定されないが、用いる含脂大豆としては、少なくとも脂質を15重量以上、好ましくは20重量%以上含むものが適当である。原料の形態は、半割れ大豆、グリッツ、粉末の形状でありうる。
 過度に脱脂され脂質含量が少なすぎると本発明に用いられる脂質に富む大豆乳化組成物を得ることが困難となる。特にヘキサン等の有機溶媒で抽出され、中性脂質の含量が1重量%以下となった脱脂大豆は、大豆の良い風味が損なわれ好ましくない。
○ Raw material soybean and its processing As the raw material of the soybean emulsified composition, fat-containing soybeans such as full-fat soybeans or partially defatted soybeans are used. Examples of partially defatted soybeans include those obtained by partially defatting whole fat soybeans by physical extraction treatment such as compression extraction. Generally, fat is contained in about 20 to 30% by weight of dry matter in full fat soybeans, and special soybean varieties have lipids of 30% by weight or more, and are not particularly limited. A material containing at least 15% by weight or more, preferably 20% by weight or more of lipid is suitable. The form of the raw material may be in the form of half cracked soybeans, grits, powder.
If it is excessively defatted and the lipid content is too small, it will be difficult to obtain a soybean-emulsified composition rich in lipids used in the present invention. In particular, defatted soybeans extracted with an organic solvent such as hexane and having a neutral lipid content of 1% by weight or less are not preferable because the good flavor of soybeans is impaired.
 上記含脂大豆は天然の状態では蛋白質の多くが未変性で可溶性の状態にあり、NSIとしては通常90を超えるが、本発明においては、NSIが20~77好ましくは20~70になるよう加工処理を施した加工大豆を用いるのが適当である。より好ましいNSIの下限値は40以上、より好ましくは41以上、さらに好ましくは43以上、最も好ましくは45以上とすることができる。より好ましいNSIの上限値は75未満、より好ましくは70未満とすることができ、またさらに65未満、あるいは60未満、あるいは58未満の低NSIのものを用いることができる。
 そのような加工大豆は、加熱処理やアルコール処理等の加工処理を行って得られる。加工処理の手段は特に限定されないが、例えば乾熱処理、水蒸気処理、過熱水蒸気処理、マイクロ波処理等による加熱処理や、含水エタノール処理、高圧処理、およびこれらの組み合わせ等が利用できる。
In the above-mentioned fat-containing soybean, most of the protein is in an unmodified and soluble state in the natural state, and the NSI is usually over 90, but in the present invention, the NSI is 20 to 77, preferably 20 to 70. It is appropriate to use processed soybeans that have been treated. A more preferable lower limit of NSI can be 40 or more, more preferably 41 or more, still more preferably 43 or more, and most preferably 45 or more. A more preferable upper limit of NSI can be less than 75, more preferably less than 70, and a lower NSI of less than 65, alternatively less than 60, or less than 58 can be used.
Such processed soybeans are obtained by performing processing such as heat treatment or alcohol treatment. The processing means is not particularly limited, and for example, heat treatment such as dry heat treatment, steam treatment, superheated steam treatment, microwave treatment, hydrous ethanol treatment, high pressure treatment, and combinations thereof can be used.
 NSIが低すぎると、大豆乳化組成物中の蛋白質の割合が高くなりやすく、蛋白質に対する脂質含量が低くなる。また過加熱による焙煎臭等の雑味が生じやすくなる。逆にNSIが例えば80以上の高い数値になると大豆乳化組成物中の蛋白質の割合が低下し、大豆からの脂質の回収率も低下しやすくなる。また風味は青臭みが強くなる。
 例えば過熱水蒸気による加熱処理を行う場合、その処理条件は製造環境にも影響されるため一概に言えないが、おおよそ120~250℃の過熱水蒸気を用いて5~10分の間で加工大豆のNSIが上記範囲となるように処理条件を適宜選択すれば良く、加工処理に特段の困難は要しない。簡便には、NSIが上記範囲に加工された市販の大豆を用いることもできる。
When NSI is too low, the ratio of the protein in the soybean emulsified composition tends to be high, and the lipid content to the protein is low. Moreover, miscellaneous taste such as roasting odor due to overheating tends to occur. Conversely, when the NSI is a high value of 80 or more, for example, the protein ratio in the soybean emulsified composition decreases, and the lipid recovery rate from soybean tends to decrease. In addition, the flavor has a strong blue odor.
For example, when heat treatment with superheated steam is performed, the treatment conditions are influenced by the production environment, so it cannot be said unconditionally, but the NSI of processed soybeans is used for 5 to 10 minutes using superheated steam at approximately 120 to 250 ° C. The processing conditions may be selected as appropriate so that the value falls within the above range, and no particular difficulty is required for the processing. For convenience, commercially available soybeans with NSI processed in the above range can also be used.
 なお、NSIは所定の方法に基づき、全窒素量に占める水溶性窒素(粗蛋白)の比率(重量%)で表すことができ、本発明においては以下の方法に基づいて測定された値とする。
 すなわち、試料2.0gに100mlの水を加え、40℃にて60分攪拌抽出し、1400×gにて10分間遠心分離し、上清1を得る。残った沈殿に再度100mlの水を加え、40℃にて60分攪拌抽出し、1400×gにて10分遠心分離し、上清2を得る。上清1および上清2を合わせ、さらに水を加えて250mlとする。No.5Aろ紙にてろ過したのち、ろ液の窒素含量をケルダール法にて測定する。同時に試料中の窒素含量をケルダール法にて測定し、ろ液として回収された窒素(水溶性窒素)の試料中の全窒素に対する割合を重量%として表したものをNSIとする。
NSI can be expressed as a ratio (% by weight) of water-soluble nitrogen (crude protein) in the total amount of nitrogen based on a predetermined method. In the present invention, NSI is a value measured based on the following method. .
That is, 100 ml of water is added to 2.0 g of a sample, followed by stirring and extraction at 40 ° C. for 60 minutes, followed by centrifugation at 1400 × g for 10 minutes to obtain supernatant 1. 100 ml of water is added again to the remaining precipitate, followed by stirring and extraction at 40 ° C. for 60 minutes, and centrifugation at 1400 × g for 10 minutes to obtain supernatant 2. Supernatant 1 and supernatant 2 are combined, and water is further added to make 250 ml. After filtering with No. 5A filter paper, the nitrogen content of the filtrate is measured by Kjeldahl method. At the same time, the nitrogen content in the sample is measured by the Kjeldahl method, and the ratio of the nitrogen recovered as filtrate (water-soluble nitrogen) to the total nitrogen in the sample is expressed as weight%, which is NSI.
 前記の加工大豆は水抽出の前に、予め乾式又は湿式による粉砕、破砕、圧偏等の組織破壊処理を施されることが好ましい。組織破壊処理に際して、あらかじめ水浸漬や蒸煮により膨潤させても良く、これによって組織破壊に必要なエネルギーを低減させたり、ホエー蛋白質やオリゴ糖等の不快味を持つ成分を溶出させ除去できると共に、保水性やゲル化性の能力が高いグロブリン蛋白質(特にグリシニン及びβ-コングリシニン)の全蛋白質に対する抽出比率、すなわち水溶性画分への移行比率をより高めることができる。 It is preferable that the processed soybean is subjected to a tissue destruction treatment such as dry or wet pulverization, crushing, and pressure bias in advance before water extraction. In tissue destruction treatment, it may be swollen in advance by water immersion or steaming, thereby reducing the energy required for tissue destruction, and eluting and removing components with unpleasant taste such as whey protein and oligosaccharides, as well as water retention The extraction ratio of globulin proteins (especially glycinin and β-conglycinin) having a high ability and gelling ability to the total protein, that is, the transfer ratio to the water-soluble fraction can be further increased.
○原料大豆からの水抽出
 水抽出は含脂大豆に対して3~20重量倍、好ましくは4~15重量倍程度の加水をし、含脂大豆を懸濁させて行われる。加水倍率は高い方が水溶性成分の抽出率が高まり、分離を良くすることができるが、高すぎると濃縮が必要となりコストがかかる。また、抽出処理を2回以上繰り返すと水溶性成分の抽出率をより高めることができる。
○ Water extraction from raw soybeans Water extraction is carried out by adding 3 to 20 times, preferably 4 to 15 times, the amount of water to fat-containing soybeans and suspending the fat-containing soybeans. The higher the rate of hydrolysis, the higher the extraction rate of the water-soluble component and the better the separation. However, if it is too high, concentration is required and costs increase. Further, when the extraction process is repeated twice or more, the extraction rate of the water-soluble component can be further increased.
 抽出温度には特に制限はないが、高い方が水溶性成分の抽出率が高まる反面、油脂も可溶化されやすくなり、大豆乳化組成物の脂質が低くなるため、70℃以下、好ましくは55℃以下で行うと良い。あるいは5~80℃、好ましくは50~75℃の範囲で行うこともできる。 The extraction temperature is not particularly limited. However, the higher the extraction rate of the water-soluble component, the more easily the oil and fat are solubilized, and the lower the lipid of the soybean emulsified composition. The following should be done. Alternatively, it can be carried out in the range of 5 to 80 ° C., preferably 50 to 75 ° C.
 抽出pH(加水後の大豆懸濁液のpH)も温度と同様に高いほうが水溶性成分の抽出率が高まる反面、油脂も可溶化されやすくなり、大豆乳化組成物の脂質が低くなる傾向にある。逆にpHが低すぎると蛋白質の抽出率が低くなる傾向にある。具体的には下限をpH6以上、もしくはpH6.3以上、もしくはpH6.5以上に調整して行うことができる。また上限は脂質の分離効率を上げる観点でpH9以下、もしくはpH8以下、もしくはpH7以下に調整して行うことができる。あるいは蛋白質の抽出率を高める観点でpH9~12のよりアルカリ性側に調整して行うことも可能である。 The higher the extraction pH (the pH of the soybean suspension after the addition of water), the higher the temperature, the higher the extraction rate of the water-soluble component, but the fats and oils also tend to be solubilized, and the lipid in the soybean emulsion composition tends to decrease. . Conversely, if the pH is too low, the protein extraction rate tends to be low. Specifically, the lower limit can be adjusted to pH 6 or higher, pH 6.3 or higher, or pH 6.5 or higher. The upper limit can be adjusted to pH 9 or lower, pH 8 or lower, or pH 7 or lower from the viewpoint of increasing lipid separation efficiency. Alternatively, from the viewpoint of increasing the extraction rate of the protein, it is possible to adjust the pH to 9 to 12 more alkaline.
○水抽出後の固液分離
 水抽出後、含脂大豆の懸濁液を遠心分離、濾過等により固液分離する。この際、中性脂質のみならず極性脂質も含めた大部分の脂質を水抽出物中に溶出させず、不溶化した蛋白質や食物繊維質の方に移行させ沈殿側(不溶性画分)とすることが重要である。具体的には含脂大豆の脂質の70重量%以上を沈殿側に移行させる。また抽出の際に上清側にも少量の脂質が溶出するが、豆乳中の脂質のように微細にエマルション化されたものではなく、15,000×g以下、あるいは5,000×g程度以下の遠心分離によっても容易に浮上させ分離することができ、この点で遠心分離機を使用するのが好ましい。なお遠心分離機は使用する設備によっては10万×g以上の超遠心分離を使用することも可能であるし、本発明に用いられる本大豆乳化組成物の場合は超遠心分離機を用いなくとも実施が可能である。
 また水抽出の際あるいは水抽出後に解乳化剤を添加して豆乳からの脂質の分離を促進させることも可能であり、解乳化剤は特に限定されないが例えば日本国出願(特願2011-108598号)に記載の特許文献2などにも引用されている解乳化剤を使用すればよい。ただし本大豆乳化組成物を調製する場合は解乳化剤を用いなくとも実施が可能である。
○ Solid-liquid separation after water extraction After water extraction, the suspension of fat-containing soybeans is subjected to solid-liquid separation by centrifugation, filtration or the like. At this time, not only neutral lipids but also most of the lipids including polar lipids are not eluted in the water extract, but transferred to the insolubilized protein or dietary fiber to make the precipitation side (insoluble fraction). is important. Specifically, 70% by weight or more of the lipid of the fat-containing soybean is transferred to the precipitation side. In addition, a small amount of lipid is also eluted on the supernatant side during extraction, but it is not finely emulsified like the lipid in soy milk, and it is centrifuged at 15,000 xg or less, or about 5,000 xg or less. In this respect, it is preferable to use a centrifuge. Depending on the equipment used, the centrifugal separator can be used for ultracentrifugation of 100,000 × g or more, and in the case of the present soybean emulsified composition used in the present invention, an ultracentrifugal separator is not required. Implementation is possible.
It is also possible to add a demulsifier at the time of water extraction or after water extraction to promote the separation of lipids from soy milk. The demulsifier is not particularly limited, but for example in Japanese application (Japanese Patent Application No. 2011-108598) Demulsifiers cited in Patent Document 2 described herein may be used. However, the preparation of the soybean emulsified composition can be carried out without using a demulsifier.
 水抽出工程後の固液分離により、中性脂質のみならず極性脂質を不溶性画分に移行させ、これを回収することにより本大豆乳化組成物の画分を得ることができる。
 固液分離として遠心分離を用いる場合、二層分離方式、三層分離方式のいずれも使用することができる。二層分離方式の場合は沈殿層である不溶性画分を回収する。また三層分離方式を用いる場合は、(1)浮上層(脂質を含む比重の最も小さいクリーム画分)、(2)中間層(脂質が少なく蛋白質、糖質を多く含む水溶性画分)、(3)沈殿層(脂質と食物繊維を多く含む不溶性画分)、の三層の画分に分けられる。この場合、脂質含量の少ない水溶性画分の中間層(2)を除去又は回収し、不溶性画分として浮上層(1)又は沈殿層(3)を回収するか、あるいは(1)と(3)を合わせて回収するとよい。
By solid-liquid separation after the water extraction step, not only neutral lipids but also polar lipids are transferred to an insoluble fraction, and the fraction of the present emulsion composition can be obtained by collecting this.
When centrifugation is used as the solid-liquid separation, either a two-layer separation method or a three-layer separation method can be used. In the case of the two-layer separation method, an insoluble fraction that is a precipitation layer is collected. When using the three-layer separation method, (1) floating layer (cream fraction with the smallest specific gravity containing lipid), (2) intermediate layer (water-soluble fraction containing a small amount of protein and carbohydrate), (3) It is divided into three-layer fractions: a sediment layer (insoluble fraction rich in lipids and dietary fibers). In this case, the intermediate layer (2) of the water-soluble fraction having a low lipid content is removed or recovered, and the floating layer (1) or the precipitated layer (3) is recovered as an insoluble fraction, or (1) and (3 ).
 得られた不溶性画分(1)、(3)はそのまま、あるいは必要により濃縮工程、加熱殺菌工程、粉末化工程等を経て本大豆乳化組成物とすることができる。 The obtained insoluble fractions (1) and (3) can be used as they are, or, if necessary, through the concentration step, the heat sterilization step, the powdering step, etc. to obtain the present soybean emulsified composition.
○食物繊維の除去
 得られた不溶性画分が食物繊維を含む場合、例えば上記(3)又は(1)及び(3)の画分である場合、必要により加水し、高圧ホモゲナイザーあるいはジェットクッカー加熱機等による均質化した後、該均質化液をさらに固液分離して上清を回収する工程を経ることにより、食物繊維(オカラ)を除去することもでき、コクのある風味がより濃縮された本大豆乳化組成物を得ることができる。該均質化の前後いずれかにおいて必要により加熱処理工程、アルカリ処理工程等を付加することにより蛋白質をより抽出しやすくすることもできる。この場合、乾物あたりの食物繊維含量は10重量%以下であり、5重量%以下がより好ましい。なお、本発明において食物繊維含量は、「五訂増補日本食品標準成分表」(文部科学省、2005)に準じ、酵素-重量法(プロスキー変法)により測定することができる。
○ Removal of dietary fiber When the obtained insoluble fraction contains dietary fiber, for example, if it is the fraction of (3) or (1) and (3) above, water is added if necessary, and a high-pressure homogenizer or jet cooker heater After homogenization by means of, etc., through the process of further solid-liquid separation of the homogenized liquid and collecting the supernatant, dietary fiber (ocara) can also be removed, and the rich flavor is further concentrated This soybean emulsified composition can be obtained. Proteins can be more easily extracted by adding a heat treatment step, an alkali treatment step or the like, if necessary, before or after the homogenization. In this case, the dietary fiber content per dry matter is 10% by weight or less, and more preferably 5% by weight or less. In the present invention, the dietary fiber content can be measured by the enzyme-weight method (modified Prosky method) according to the “Fifth Supplement Japanese Food Standard Ingredient Table” (Ministry of Education, Culture, Sports, Science and Technology, 2005).
(大豆乳化組成物の特徴)
 本大豆乳化組成物は、脂質(中性脂質及び極性脂質)及び蛋白質が特定の範囲で含まれ、蛋白質のうち特にLP含量が高く、必要により繊維質も含まれる乳化組成物であり、大豆が本来有する自然な美味しさが濃縮されており、従来の問題とされていた青臭味や収斂味、渋味等の不快味がないか非常に少なく、非常にコクのある風味を有するものである。
 通常の大豆粉や分離大豆蛋白に水、油脂を加えて本大豆乳化組成物と類似の組成の乳化組成物にすることは可能であるが、リポキシゲナーゼ蛋白質含量あるいはLCI値を同等なレベルに調整することは困難である
。そして本技術により調製された大豆乳化組成物は、このような組み立て製品に比べて格段に風味が良好であり、食品素材としての利用適性が高いことに特徴を有する。
(Characteristics of soybean emulsified composition)
The present soybean emulsified composition is an emulsified composition that contains lipids (neutral lipids and polar lipids) and proteins in a specific range, has a particularly high LP content of proteins, and also contains fibers as necessary. The natural taste that is inherently concentrated is concentrated, and there is no or very little unpleasant taste such as blue odor, astringent taste, astringency, etc., which has been regarded as a conventional problem, and it has a very rich flavor. .
It is possible to add water and fat to normal soy flour and isolated soy protein to make an emulsified composition similar to this soy emulsified composition, but adjust the lipoxygenase protein content or LCI value to the equivalent level It is difficult. And the soybean emulsified composition prepared by this technique is characterized by remarkably good flavor and high suitability for use as a food material compared to such an assembled product.
 本大豆乳化組成物における脂質は、未精製大豆油であることが風味の点からも望ましい。すなわち、本大豆乳化組成物における脂質は、原料である丸大豆に由来する場合が最も好ましい結果を得ることができるのであり、丸大豆から所定の方法で調製された本大豆乳化組成物が、例えば本第五発明の血清コレステロール低減用組成物の作用本体として最も好ましいものである。 It is desirable from the viewpoint of flavor that the lipid in the present soybean emulsified composition is unrefined soybean oil. That is, the most preferable result can be obtained when the lipid in the present soybean emulsified composition is derived from the whole soybean, which is the raw material, and the present soybean emulsion prepared from the whole soybean by a predetermined method is, for example, It is most preferable as the action body of the composition for reducing serum cholesterol of the fifth invention.
<大豆由来原料含有飲食品>
 本発明における大豆由来原料含有飲食品は、広義には後述する本第一~第五発明に記載のように大豆由来原料を乳代替組成物、酸性大豆素材、卵黄代替組成物、大豆加工食品用の原料、血清コレステロール低減用もしくは腎機能改善用組成物として使用した食品などを包含する概念である。
<Soy-derived raw material-containing food and drink>
The soy-derived raw material-containing food / beverage in the present invention is broadly defined as a milk substitute composition, an acidic soybean material, an egg yolk substitute composition, or a processed soybean food as described in the first to fifth inventions described later. And the food used as a composition for reducing serum cholesterol or improving renal function.
 以下、本大豆乳化組成物を特別な技術的特徴とする本第一~第五発明の実施形態について、それぞれ具体的に説明する。 Hereinafter, the embodiments of the first to fifth inventions having the special technical characteristics of the soybean emulsified composition will be specifically described.
<第一発明の実施形態>
 本第一発明に用いられる本大豆乳化組成物は、上記の特徴を持つものであり、従来の豆腐、豆乳及び大豆乳化組成物に対比して、大豆特有の青臭みがなく、独特のコク味を有する。従って、大豆由来製品はコク味、旨味に乏しいという従来の常識を超えて、本大豆乳化組成物は、牛乳や生クリームなどの乳製品を代替しうる素材として、広く洋菓子類、パン類、デザート類、飲料類、スープ類、ソース類などの生クリームなどの乳製品が使用される飲食品に利用することができる。
<Embodiment of the first invention>
The present soybean emulsified composition used in the present first invention has the above-mentioned characteristics, and has no blue smell peculiar to soybeans and a unique richness compared to conventional tofu, soymilk and soy emulsified compositions. Have Therefore, the soy emulsified composition is widely used as a substitute for dairy products such as milk and fresh cream. It can be used for foods and drinks in which dairy products such as fresh creams such as foods, beverages, soups and sauces are used.
 なお、本大豆乳化組成物は乾燥して粉末状大豆乳化組成物として、全脂粉乳を代替する食品原材料として本第一発明の大豆由来原料含有飲食品に利用することも可能である。本第一発明は、かかる粉末状大豆乳化組成物及びそれを利用した大豆由来原料含有食品も包含するものである。 In addition, this soybean emulsified composition can be dried and used as a powdered soybean emulsified composition as a food raw material that substitutes whole milk powder for the food and beverage containing the soybean-derived material of the first invention. The first invention includes such a powdery soybean emulsified composition and a soybean-derived raw material-containing food using the same.
 本第一発明は、本大豆乳化組成物を乳製品代替物として含有する大豆由来原料含有飲食品である。より詳しくは本第一発明の大豆由来原料含有飲食品とは、牛乳、生クリーム、全脂粉乳などの乳製品が利用される洋菓子用またはパン用原材料、洋菓子類、パン類、デザート類、飲料類、スープ類、ソース類などにおいて、使用される乳製品の一部又は全部が本大豆乳化組成物に置換されたものを意味する。 This 1st invention is a soybean origin raw material containing food / beverage product which contains this soybean emulsion composition as a dairy product substitute. More specifically, the soy-derived raw material-containing food and drink of the first invention refers to a confectionery or bakery ingredient in which dairy products such as milk, fresh cream, and whole milk powder are used, confectionery, breads, desserts, beverages In soups, soups, sauces, etc., it means that part or all of the dairy product used is replaced with the present soybean emulsified composition.
 具体的には、洋菓子用またはパン用原材料としては、ケーキ、パン類のトッピング、コーチング、サンド、フィリング及び練り込みのいずれかの1目的以上に用いられるホイップクリームなどの水中油型乳化組成物、マーガリン、ファットスプレッドなどの油中水型乳化組成物、カスタードクリームなどのフラワーペースト類、ガナッシュや生チョコレートなどの含水チョコレート類、流動状、ペースト状、固形状のチーズ様食品類などを意味する。 Specifically, as a raw material for confectionery or bread, an oil-in-water emulsion composition such as whipped cream used for one or more of cake, bread topping, coaching, sand, filling and kneading, It means water-in-oil emulsion compositions such as margarine and fat spread, flower pastes such as custard cream, water-containing chocolates such as ganache and raw chocolate, fluid, pasty and solid cheese-like foods.
 洋菓子類及びパン類とは、上記の洋菓子用またはパン用原材料を使用したショートケーキやチーズケーキなどの洋菓子及びパン全般を意味する。デザート類としては、上記の洋菓子用またはパン用原材料の1種以上をトッピング、コーチング、サンド、フィリング及び練り込みのいずれかの1目的以上に用いたゼリー、ババロア、プリン、冷菓、アイスクリームなどを意味する。 Western confectionery and bread means general confectionery and bread such as short cakes and cheesecakes using the above-mentioned ingredients for pastry or bread. Desserts include jelly, bavaroa, pudding, frozen confectionery, ice cream, etc. used for one or more of topping, coaching, sanding, filling, and kneading one or more of the above-mentioned ingredients for Western confectionery or bread means.
 飲料類としては、牛乳、生クリーム、全脂粉乳などの乳製品が利用される乳飲料、発酵飲料、果汁飲料、コーヒークリームのなど乳製品の一部又は全部が本大豆乳化組成物に置換されたものを意味する。 As the beverages, a part or all of the dairy products such as milk, fresh cream, whole milk powder, dairy beverages, fermented beverages, fruit juice beverages, coffee creams, etc. are replaced with the present soybean emulsified composition. Means something.
 スープ類及びソース類としては、牛乳、生クリーム、全脂粉乳などの乳製品が利用されるスープ、ソース類全般において、乳製品の一部又は全部が本大豆乳化組成物に置換されたものを意味し、具体的にはスープ、シチュー、グラタン、ホワイトソースなどを意味する。 Soups and sauces include soups that use dairy products such as milk, fresh cream, whole milk powder, etc., in which all or part of the dairy product is replaced with the present soybean emulsified composition. Specifically, it means soup, stew, gratin, white sauce and so on.
 本第一発明は、上記の風味改善された大豆由来原料含有飲食品、すなわち、洋菓子用及びパン用原材料、洋菓子類、パン類、デザート類、飲料類、スープ類、ソース類などへの本大豆乳化組成物の乳製品代替物としての使用である。本大豆乳化組成物の使用量はその風味的特徴が明確に出る量であれば特に制限はないが、概ね乾物換算で大豆由来原料含有飲食品に対し0.5重量%以上、好ましくは1~30重量%、最も好ましくは1~20重量%である。下限未満では、本大豆乳化組成物の風味的特徴が明確に出ないため、好ましくない。 This first invention is the above-mentioned flavor-improved soy-derived raw material-containing food or drink, that is, the present soybean for confectionery and bread ingredients, confectionery, breads, desserts, beverages, soups, sauces, etc. Use of an emulsified composition as a dairy substitute. The amount of the soybean emulsified composition used is not particularly limited as long as its flavor characteristics are clearly shown, but is generally 0.5% by weight or more, preferably 1 to 30% by weight, most preferably 1-20% by weight. If it is less than the lower limit, the flavor characteristics of the present soybean emulsified composition are not clearly obtained, and therefore it is not preferable.
 また、本大豆乳化組成物の使用において、糖類、デンプン類、油脂、食塩、乳化剤、乳化安定剤、増粘剤、調味料、酸味料、香辛料、着色料、酸化防止剤などの1種以上を適宜併用することができる。 Further, in the use of the present soybean emulsified composition, at least one kind of sugar, starch, fats and oils, salt, emulsifier, emulsion stabilizer, thickener, seasoning, acidulant, spice, colorant, antioxidant, etc. It can be used together as appropriate.
 糖類としては、メープルシロップ、はちみつ、黒糖、グラニュー糖、マルトース、トレハロース、マルチトースなどが例示でき、デンプン類としては生デンプン、加水分解されたデンプン類、加工デンプン類を例示できる。 Examples of sugars include maple syrup, honey, brown sugar, granulated sugar, maltose, trehalose, and maltose. Examples of starches include raw starch, hydrolyzed starch, and modified starch.
 油脂としては、特に制限はないが、ココアバター、ナタネ油、大豆油、ヒマワリ油、綿実油、落花生油、米糠油、コーン油、サフラワー油、オリーブ油、ゴマ油、パーム油、シア脂、サル脂、ヤシ油、パーム核油等の植物性油脂及び乳脂、牛脂、豚脂、魚油等の動物性油脂、上記油脂の単独若しくは混合油、又はそれらの硬化、分別、エステル交換等を施した加工油脂の1種以上を例示できる。 The oil and fat is not particularly limited, but cocoa butter, rapeseed oil, soybean oil, sunflower oil, cottonseed oil, peanut oil, rice bran oil, corn oil, safflower oil, olive oil, sesame oil, palm oil, shea fat, monkey fat, Of vegetable oils and milk fats such as coconut oil and palm kernel oil, animal fats and oils such as beef tallow, pork fat and fish oil, single or mixed oils of the above fats, or processed fats and oils which have been cured, fractionated, transesterified, etc. One or more types can be exemplified.
 乳化剤としては、レシチン、蔗糖脂肪酸エステル、グリセリン脂肪酸エステル、ポリグリセリン脂肪酸エステル、ソルビタン脂肪酸エステル、ジアセチル酒石酸モノグリセリドなどが例示できる。 Examples of the emulsifier include lecithin, sucrose fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, sorbitan fatty acid ester, diacetyltartaric acid monoglyceride and the like.
 乳化安定剤及び増粘剤としては、水溶性大豆多糖類、プルラン、カルボキシメチルセルロース、寒天、ゼラチン、ペクチン、カラギーナン、グアガム、ローカストビーンガム、アルギン酸ナトリウムなどが例示できる。 Examples of the emulsion stabilizer and thickener include water-soluble soybean polysaccharide, pullulan, carboxymethylcellulose, agar, gelatin, pectin, carrageenan, guar gum, locust bean gum, sodium alginate and the like.
 香辛料としては、ターメリック、からし、胡椒などが例示できる。着色料としては、カロテンやリコペンなどの植物色素や各種合成色素を利用することができる。 Examples of spices include turmeric, mustard and pepper. As the coloring agent, plant pigments such as carotene and lycopene and various synthetic pigments can be used.
 また、酸化防止剤として、トコフェロール類、カテキン類、ルチンなどのフラボン類、アスコルビン酸誘導体類、ローズマリーやヤマモモ抽出物のような植物抽出物類などが使用できる。 Further, as antioxidants, flavones such as tocopherols, catechins and rutin, ascorbic acid derivatives, plant extracts such as rosemary and bayberry extracts can be used.
 また、本第一発明は、大豆由来組成物を乳製品代替物として含有する洋菓子類、パン類、デザート類、飲料類、スープ類、ソース類などの大豆由来原料含有飲食品の風味を改善する方法である。従来の大豆由来素材より風味が大幅に改善された本大豆乳化組成物を使用することにより、洋菓子類、パン類、デザート類、飲料類、スープ類、ソース類などの風味を大幅に改善することができ、牛乳、生クリーム、全脂粉乳などの乳製品と一部又は全部置換することにより風味に優れた飲食品の提供を可能とする。 Moreover, this 1st invention improves the flavor of food-drinks containing soybean origin raw materials, such as confectionery, breads, desserts, beverages, soups, sauces, etc. which contain a soybean origin composition as a dairy product substitute. Is the method. Significantly improve the flavor of pastry, bakery, desserts, beverages, soups, sauces, etc. by using this soy emulsified composition that has a significantly improved flavor compared to conventional soybean-derived ingredients It is possible to provide a food or drink excellent in flavor by substituting part or all with milk products such as milk, fresh cream and whole milk powder.
 また、本第一発明は、大豆由来組成物を含有する洋菓子類、パン類、デザート類、飲料類、スープ類、ソース類などの大豆由来原料含有飲食品の製造において、風味が大幅に改善された大豆由来原料含有飲食品の製造法である。牛乳、生クリーム、全脂粉乳などの乳製品の一部又は全部を本大豆乳化組成物と置換することにより、青くさみがなく大豆由来のコクのある大豆由来原料含有飲食品を容易に製造することができる。 In addition, in the first invention of the present invention, the flavor is greatly improved in the production of foods and beverages containing soy-derived ingredients such as confectionery, breads, desserts, beverages, soups, and sauces containing soy-derived compositions. It is the manufacturing method of the food-drinks containing the raw material derived from soybean. By replacing a part or all of dairy products such as milk, fresh cream and whole milk powder with this soy emulsified composition, it is easy to produce soy-derived raw-material-containing foods that are free from blueness and have richness derived from soy. can do.
<第二発明の実施形態>
(酸性大豆素材)
 本大豆乳化組成物を含む原料を乳酸発酵又は酸添加により酸性化して得られる酸性大豆素材及びその製造法について説明する。
<Embodiment of the second invention>
(Acid soy material)
The acidic soybean raw material obtained by acidifying the raw material containing this soybean emulsified composition by lactic acid fermentation or acid addition, and its manufacturing method are demonstrated.
○原料
 本第二発明の酸性大豆素材の原料は、本大豆乳化組成物を含むことが特徴である。本大豆乳化組成物を使用することにより、従来の豆乳や分離大豆蛋白を乳酸発酵させたときに感ずる劣化臭や酢酸臭及び収斂味といった好ましくない風味が非常に少ない酸性大豆素材を得ることができる。
 原料中の本大豆乳化組成物の含量は、30~100重量%、好ましくは60~100重量%、より好ましくは90~100重量%であることができる。
 原料中の乾物あたりの蛋白質含量は、7重量%以上、好ましくは15重量%以上、より好ましくは20重量%以上が適当であり、50重量%以下が好ましい。
 原料中の乾物あたりの脂質含量は、10重量%以上、好ましくは20重量%以上、より好ましくは30重量%以上が適当であり、75重量%以下が好ましい。また原料中の蛋白質あたりの脂質含量は、100重量%以上が好ましく、120~250重量%がより好ましく、120~200重量%がさらに好ましい。
 原料中に含まれる大豆蛋白質のLCI値は副原料の添加によっても変わるが、55%以上が適当であり、好ましくは58%以上であり、より好ましくは60%以上であり、さらに好ましくは63%以上、最も好ましくは65%以上である。
 原料の水分含量は得られる酸性大豆素材の硬さに影響し、好みの品質にあわせて適宜設定することができ、参考までに40~95重量%とすることができる。
Raw material The raw material of the acidic soybean material of the second invention is characterized by containing the present soybean emulsified composition. By using the present soybean emulsified composition, it is possible to obtain an acidic soy material with very little unfavorable flavor such as deteriorated odor, acetic acid odor and astringent taste felt when lactic fermentation of conventional soy milk or isolated soy protein. .
The content of the present soybean emulsified composition in the raw material can be 30 to 100% by weight, preferably 60 to 100% by weight, more preferably 90 to 100% by weight.
The protein content per dry matter in the raw material is suitably 7% by weight or more, preferably 15% by weight or more, more preferably 20% by weight or more, and preferably 50% by weight or less.
The lipid content per dry matter in the raw material is suitably 10% by weight or more, preferably 20% by weight or more, more preferably 30% by weight or more, and preferably 75% by weight or less. The lipid content per protein in the raw material is preferably 100% by weight or more, more preferably 120 to 250% by weight, and still more preferably 120 to 200% by weight.
The LCI value of soy protein contained in the raw material varies depending on the addition of the auxiliary raw material, but 55% or more is appropriate, preferably 58% or more, more preferably 60% or more, and further preferably 63%. Above, most preferably 65% or more.
The moisture content of the raw material affects the hardness of the resulting acidic soy material, and can be set as appropriate according to the desired quality, and can be 40 to 95% by weight for reference.
 本第二発明の酸性大豆素材の原料には、本大豆乳化組成物以外に糖類、油脂、澱粉、蛋白質、pH調整剤、増粘多糖類、ゲル化剤、乳化剤、香料、酸味料、酸化防止剤、キレート剤等の原料や添加剤を適宜加えることができる。 In addition to the soybean emulsified composition, the raw material for the acidic soybean material of the second invention includes sugars, fats and oils, starches, proteins, pH adjusters, thickening polysaccharides, gelling agents, emulsifiers, fragrances, acidulants, and antioxidants. Raw materials and additives such as agents and chelating agents can be added as appropriate.
 乳酸発酵による酸性化の場合、乳酸菌の栄養源として、必要に応じて資化性糖類を加えてもよい。例えばグルコース、フラクトース、ショ糖、マルトース、ガラクトース、ラクトース、ラフィノース、トレハロース、大豆オリゴ糖、フラクトオリゴ糖、キシロオリゴ糖等を用いることができる。これら糖原料は単独でも良いし、2種類以上を組み合わせてもよい。資化性糖類の配合量は、本大豆乳化組成物の固形分に対して0~50重量%が適当であり、0~20重量%が好ましい。 In the case of acidification by lactic acid fermentation, assimilable saccharides may be added as a nutrient source for lactic acid bacteria as necessary. For example, glucose, fructose, sucrose, maltose, galactose, lactose, raffinose, trehalose, soybean oligosaccharide, fructooligosaccharide, xylooligosaccharide and the like can be used. These sugar raw materials may be used alone or in combination of two or more. The amount of the assimilating saccharide is suitably 0 to 50% by weight, preferably 0 to 20% by weight, based on the solid content of the soybean emulsified composition.
 本第二発明の酸性大豆素材の脂質量は本大豆乳化組成物に由来する脂質量で十分にコクが付与されたものである。そしてさらに脂質を増やしてよりコクを増強したり、酸性大豆素材のボディの硬さ調整等のため、油脂を別途加えることができる。これによって酸性大豆素材中の脂質含量を3~40重量%にも調整できる。
 添加する油脂としては、動植物性油脂又はこれらの硬化油、分別油、エステル交換油、ジグリセリド、中鎖脂肪酸含有油脂などの加工油脂から選択される一種又は2種以上を使用することが出来る。動植物性油脂の例としては大豆油、菜種油、米油、ヒマワリ油、サフラワー油、パーム油、パーム核油、ヤシ油、コーン油、綿実油、落花生油、サル脂、シア脂、牛脂、乳脂、豚脂、カカオ脂、魚油、鯨油、からし油等が挙げられるが、植物性油脂の使用が好ましい。特に口溶けの良好な食感を付与する観点から、パーム分別油、菜種硬化油などが好ましい。油脂の融点は製品の硬さを考慮し適宜選択できるが、5~40℃が好ましい。
The amount of lipid in the acidic soybean material of the second invention is the amount of lipid derived from the present soybean emulsified composition and sufficiently rich. In addition, fats and oils can be added separately in order to increase the fat by further increasing the fat, or to adjust the hardness of the body of the acidic soybean material. As a result, the lipid content in the acidic soybean material can be adjusted to 3 to 40% by weight.
As fats and oils to be added, one or two or more kinds selected from processed fats and oils such as animal and vegetable fats and oils, hardened oils, fractionated oils, transesterified oils, diglycerides, and medium chain fatty acid-containing fats and oils can be used. Examples of animal and vegetable oils include soybean oil, rapeseed oil, rice oil, sunflower oil, safflower oil, palm oil, palm kernel oil, palm oil, corn oil, cottonseed oil, peanut oil, monkey fat, shea fat, beef fat, milk fat, Examples include pork fat, cacao butter, fish oil, whale oil, mustard oil, etc., but it is preferable to use vegetable oil. In particular, from the viewpoint of imparting a good mouth melt texture, palm fractionated oil, rapeseed hydrogenated oil, and the like are preferable. The melting point of fats and oils can be appropriately selected in consideration of the hardness of the product, but is preferably 5 to 40 ° C.
 本第二発明の酸性大豆素材の原料は油脂を別途添加した場合など、必要によりホモゲナイザー等の適当な均質化手段によって均質化することができる。その際の均質化圧力はクリームチーズ様食品の組織の硬さに影響するので、当業者が好みの品質に応じて適宜設定すればよいが、一般には2.5~15MPa(25~150kg/cm2)が適当である。 The raw material of the acidic soybean material of the second invention can be homogenized by an appropriate homogenizing means such as a homogenizer, if necessary, such as when fats and oils are separately added. The homogenization pressure at that time affects the hardness of the structure of the cream cheese-like food, so that it can be appropriately set according to the quality desired by those skilled in the art, but generally 2.5 to 15 MPa (25 to 150 kg / cm 2). 2 ) is appropriate.
○酸性化
 次に、上記原料を酸性化する。すなわち原料のpHを酸性に調整する。酸性化手段としては、酸を添加する方法、乳酸菌発酵する方法、あるいはそれらを組合せる方法を利用できる。一般にpH3.5~6.0、好ましくは4~5.9、より好ましくは5.0~5.8が適当である。酸添加の場合も同様のpH範囲に調整すればよい。pHが低すぎると酸味が強くなり、ざらつきが生じやすくなる。また、pHが高すぎると酸性化によるサワー感が弱まり、特に乳酸発酵による場合は発酵風味が乏しくなる。
-Acidification Next, the said raw material is acidified. That is, the pH of the raw material is adjusted to be acidic. As an acidifying means, a method of adding an acid, a method of fermenting lactic acid bacteria, or a method of combining them can be used. In general, a pH of 3.5 to 6.0, preferably 4 to 5.9, more preferably 5.0 to 5.8 is appropriate. What is necessary is just to adjust to the same pH range also in the case of acid addition. If the pH is too low, the acidity becomes strong and roughening tends to occur. Moreover, when pH is too high, the sour feeling by acidification will weaken, especially in the case of lactic acid fermentation, fermentation flavor will become scarce.
 酸添加によってpHを酸性に調整する場合、使用する酸は特に種類に限定なく使用でき、リン酸、塩酸、硫酸等の無機酸や、クエン酸、リンゴ酸、乳酸、グルコン酸、GDL等の有機酸を例示でき、これらを1種単独又は2種以上を混合して使用することができるが、風味上有機酸を用いるのが好ましい。 When adjusting pH to acid by acid addition, the acid to be used can be used without any limitation, and inorganic acids such as phosphoric acid, hydrochloric acid and sulfuric acid, and organic acids such as citric acid, malic acid, lactic acid, gluconic acid and GDL An acid can be illustrated and these can be used individually by 1 type or in mixture of 2 or more types, but it is preferable to use an organic acid on flavor.
 乳酸発酵によってpHを酸性に調整する場合、乳酸菌は通常のヨーグルト、乳酸菌飲料やチーズ等の発酵乳製品に使用されるものを使用することができ、特に限定されない。例えば、ラクトバチルス・カゼイ、ラクトバチルス・プランタラム、ラクトバチルス・ヘルベティカス、ラクトバチルス・ブルガリカス、ラクトバチルス・ガセリ、ラクトバチルス・アシドフィラス、ラクトバチルス・ラクチス、ラクトバチルス・サリバリウス・サリバリウス、ラクトバチルス・ガリナラム、ラクトバチルス・アミロボラス、ラクトバチルス・ブレビス・ブレビス、ラクトバチルス・ファーメンタム、ラクトバチルス・マリ、ラクトバチルス・デルブルッキィ、ラクトバチルス・ジョンソニー、ラクトバチルス・サンフランシスエンシス、ラクトバチルス・パネックス、ラクトバチルス・コモエンシス、ラクトバチルス・イタリカス、ラクトバチルス・ライキマニ、ラクトバチルス・カルバタス、ラクトバチルス・ヒルガルディ、ラクトバチルス・ルテリ、ラクトバチルス・パストリアヌス、ラクトバチルス・ブクネリ、ラクトバチルス・セロビオサス、ラクトバチルス・フルクティボランス、ラクトバシルス・ラクチス・サブスピーシーズ・クレモリス等のラクトバチルス属、ストレプトコッカス・サーモフィルス、ストレプトコッカス・ラクチス、ストレプトコッカス・ジアセチルラクチス等のストレプトコッカス属、ラクトコッカス・ラクチス・サブスピーシーズ・ラクチス、ラクトコッカス・ラクチス・サブスピーシーズ・クレモリス、ラクトコッカス・ラクチス・サブスピーシーズ・ジアセチラクチス、ラクトコッカス・ラクチス・サブスピーシーズ・ラクチス・バイオバー・ジアセチラクチス等のラクトコッカス属、ロイコノストック・メセンテロイデス・サブスピーシーズ・クレモリス、ロイコノストック・ラクチス、ロイコノストック・シュードメセンテオリデス等のロイコノストック属等を用いることができる。また、ケフィア菌など、乳酸菌以外の酵母等の微生物が混合されたスターターを用いることも可能である。 When the pH is adjusted to acidic by lactic acid fermentation, lactic acid bacteria can be those used for fermented dairy products such as ordinary yogurt, lactic acid bacteria beverages and cheese, and are not particularly limited. For example, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus helveticus, Lactobacillus bulgaricus, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus lactis, Lactobacillus salivalus salivaius, Lactobacillus gallinarum , Lactobacillus amylovorus, Lactobacillus brevis brevis, Lactobacillus fermentum, Lactobacillus mari, Lactobacillus delbrukki, Lactobacillus johnsonii, Lactobacillus sanfrancisensis, Lactobacillus panex, Lactobacillus Comoensis, Lactobacillus italicas, Lactobacillus raikimani, Lactobacillus carbatus, Lactobacillus hirugal Lactobacillus luteri, Lactobacillus pastorianus, Lactobacillus bukuneri, Lactobacillus cellobiosas, Lactobacillus fructibolans, Lactobacillus lactis subspecies Cremolis and other Lactobacillus genus, Streptococcus thermophilus, Streptococcus Streptococcus genus such as Lactis, Streptococcus diacetyllactis, Lactococcus lactis subspecies lactis, Lactococcus lactis subspecies cremolith, Lactococcus lactis subspices diacety lactis, Lactococcus lactis subtilis lactis・ Lactococcus genus such as biobars and diacetylactis, Leuconostoc mecenteroy Vinegar subsp. Cremoris, Leuconostoc lactis, it is possible to use a Leuconostoc such as Leuconostoc pseudotuberculosis eyes hand-woven death. It is also possible to use a starter in which microorganisms such as yeast other than lactic acid bacteria such as kefir bacteria are mixed.
 また本第二発明ではビフィドバクテリウム属も乳酸菌に含まれるものとし、例えばビフィドバクテリウム・ビフィダム、ビフィドバクテリウム・ロンガム、ビフィドバクテリウム・インファンティス、ビフィドバクテリウム・ブレーベ、ビフィドバクテリウム・アドレセンティス、ビフィドバクテリウム・アンギュラータム、ビフィドバクテリウム・カテニュラータム、ビフィドバクテリウム・シュードカテニュラータム、ビフィドバクテリウム・デンティウム、ビフィドバクテリウム・グロボズム、ビフィドバクテリウム・シュードロンガム、ビフィドバクテリウム・クニキュリ、ビフィドバクテリウム・コエリナム、ビフィドバクテリウム・アニマリス、ビフィドバクテリウム・サーモフィラム、ビフィドバクテリウム・ボウム、ビフィドバクテリウム・マグナム、ビフィドバクテリウム・アステロイデス、ビフィドバクテリウム・インディカム、ビフィドバクテリウム・ガリカム、ビフィドバクテリウム・ラクチス、ビフィドバクテリウム・イノピナータム、ビフィドバクテリウム・デンティコレンス、ビフィドバクテリウム・プローラム、ビフィドバクテリウム・スイス、ビフィドバクテリウム・ガリナーラム、ビフィドバクテリウム・ルミナンティウム、ビフィドバクテリウム・メリシカム、ビフィドバクテリウム・サーキュラーレ、ビフィドバクテリウム・ミニマム、ビフィドバクテリウム・サブチル、ビフィドバクテリウム・コリネフォルメ等が挙げられる。また、これらの乳酸菌は単独や2種類以上の任意の組み合わせでも使用することができる。 In the second invention, the genus Bifidobacterium is also included in the lactic acid bacterium, such as Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium infantis, Bifidobacterium breve, Bifidobacterium adrecentis, Bifidobacterium angularum, Bifidobacterium catenatum, Bifidobacterium pseudocatenatum, Bifidobacterium denthium, Bifidobacterium globosm, Bifidobacterium Phytobacterium pseudolongum, Bifidobacterium cuniculi, Bifidobacterium coelinum, Bifidobacterium animalis, Bifidobacterium thermophilum, Bifidobacterium boum, Bif D. magnum, Bifidobacterium asteroides, Bifidobacterium indicum, Bifidobacterium gallicum, Bifidobacterium lactis, Bifidobacterium inopinatum, Bifidobacterium denticorens, Bifidobacterium Fidobacterium prolam, Bifidobacterium swiss, Bifidobacterium gallinarum, Bifidobacterium luminantium, Bifidobacterium merlicum, Bifidobacterium circulare, Bifidobacterium minimum, Bifidobacterium Examples include Fidobacterium sabutyl and Bifidobacterium coryneform. Moreover, these lactic acid bacteria can be used individually or in arbitrary combinations of 2 or more types.
 乳酸菌の添加量は、発酵温度、発酵時間に応じて調整することができる。発酵条件は、10~50℃で3~48時間、好ましくは15~45℃で4~30時間が適当である。 発酵後のpHは嗜好に合わせてアルカリ又は酸を用いて適宜微調整することができる。 The addition amount of lactic acid bacteria can be adjusted according to fermentation temperature and fermentation time. The fermentation conditions are 10 to 50 ° C. for 3 to 48 hours, preferably 15 to 45 ° C. for 4 to 30 hours. The pH after fermentation can be fine-tuned appropriately using alkali or acid according to taste.
○ホエーの分離
 適当なpHに調整された酸性化物は、必要であれば遠心分離等によりホエーを分離してカードを回収する工程を採ることができる。ホエーを分離した場合には固形分が高く固形状ないし半固形状の適度な硬さを有するホエー分離タイプのチーズ様の酸性大豆素材を製造でき、またホエーの分離によって酸味の少ない風味に調整できる。
 またホエーを分離しない場合は固形分が低く、液状ないしペースト状のホエー非分離タイプのチーズ様の酸性大豆素材を製造できる。ホエーを分離するかしないかは当業者が製造する食品の用途に適した物性を考慮して適宜決定することができる。
○ Separation of whey Acidified products adjusted to an appropriate pH can be subjected to a step of separating the whey by centrifugation or the like and collecting the card if necessary. When whey is separated, a whey-separated cheese-like acidic soybean material having a high solid content and a solid or semi-solid hardness can be produced, and can be adjusted to a less sour taste by separating whey. .
When whey is not separated, the solid content is low, and a liquid or pasty whey non-separable cheese-like acidic soybean material can be produced. Whether or not to separate whey can be appropriately determined in consideration of the physical properties suitable for the use of the food produced by those skilled in the art.
○添加剤
 次に、上記原料の酸性化の後、上記のホエーを分離する前の酸性化物又は酸性化物からホエーを除去したカードに、必要により塩化ナトリウム、塩化カリウム等の塩化物や、ポリリン酸ナトリウム等のリン酸塩などの塩を添加することができる。これらの添加物は酸性化処理の前に添加しておくことも可能である。
 特に、ポリリン酸ナトリウム等の重合リン酸塩を添加することにより、酸味を軽減でき、またリン酸塩の添加によってコク味をより向上させ、ざらつきを軽減して滑らかな食感を増すことができるため好ましい。リン酸塩の添加量は限定されないが、酸性大豆素材中のタンパク質あたり0.5~15重量%が好ましい。リン酸塩が少なすぎるとざらつきの軽減効果が小さくなり、多すぎると収斂味が強くなる傾向となる。
○ Additives Next, after acidification of the above raw material, the acidified product before separating the whey, or a card from which the whey has been removed from the acidified product, chloride such as sodium chloride and potassium chloride as necessary, and polyphosphoric acid Salts such as phosphates such as sodium can be added. These additives can be added before the acidification treatment.
In particular, by adding a polymerized phosphate such as sodium polyphosphate, the acidity can be reduced, and the addition of phosphate can improve the richness, reduce the roughness, and increase the smooth texture. Therefore, it is preferable. The amount of phosphate added is not limited, but is preferably 0.5 to 15% by weight per protein in the acidic soybean material. If the amount of phosphate is too small, the effect of reducing the roughness is reduced, and if it is too much, the astringent taste tends to be strong.
 次に、酸性化物又はカードを必要により加熱殺菌する。加熱条件は特に限定されないが、70~85℃で1秒~15分程度が適当である。該加熱殺菌の後、必要によりホモゲナイザー等によって均質化を行い、4~10℃程度まで冷却して酸性大豆素材を得る。 Next, heat sterilize the acidified product or curd as necessary. There are no particular restrictions on the heating conditions, but a temperature of 70 to 85 ° C. for about 1 second to 15 minutes is appropriate. After the heat sterilization, if necessary, homogenization is performed with a homogenizer or the like, and the mixture is cooled to about 4 to 10 ° C. to obtain an acidic soybean material.
○チーズタイプ及びヨーグルトタイプ
 本第二発明の酸性大豆素材は、原料の種類、乳酸菌の種類や発酵条件(温度や時間等)を変え、チーズタイプの素材、あるいはヨーグルトタイプの素材にすることができる。
 チーズタイプの素材は、既存のチーズと同様の製法により得られるか、又は澱粉や油脂等の副原料を配合して既存のチーズと同様の品質(硬さや風味)を有する素材を言う。既存のチーズの種類としては、大きくナチュラルチーズとプロセスチーズに分けられる。本第二発明の代表的態様としては非熟成タイプのフレッシュチーズやクリームチーズが挙げられ、フレッシュチーズとして具体的には、カッテージチーズ、フロマージュ・ブラン、モツァレラチーズ、リコッタチーズ、マスカルポーネ、カース・マルツゥ、フェタチーズ、パニール、ルーシャン等がある。またフレッシュチーズ以外にも、ウォッシュチーズや白カビチーズ、青カビチーズ、セミハードチーズ、ハードチーズのような熟成チーズタイプの素材にすることも可能である。熟成チーズの代表例としては、ゴーダチーズ、エダムチーズ、カマンベールチーズ、チェダーチーズ等がある。
 ヨーグルトタイプの素材としては、既存のヨーグルトと同様の製法により得られるか、又は副原料を配合して既存のヨーグルトと同様の品質(物性や風味)を有する素材を言う。既存のヨーグルトの種類としては、日本の他ブルガリアヨーグルトやロシアのケフィアヨーグルトなど、世界各国で作られるヨーグルトが挙げられる。
○ Cheese type and yogurt type The acidic soybean material of the second invention of the present invention can be made into a cheese type material or a yogurt type material by changing the type of raw material, the type of lactic acid bacteria and the fermentation conditions (temperature, time, etc.). .
A cheese-type material refers to a material that is obtained by the same production method as that for existing cheeses or that has the same quality (hardness and flavor) as existing cheeses by adding auxiliary materials such as starch and fats and oils. Existing cheese types can be broadly divided into natural cheese and processed cheese. Typical examples of the second invention include non-aged type fresh cheese and cream cheese, and specifically as fresh cheese, cottage cheese, fromage blanc, mozzarella cheese, ricotta cheese, mascarpone, curse martu, There are feta cheese, paneer, luchamp. In addition to fresh cheese, it is also possible to use an aged cheese type material such as wash cheese, white mold cheese, blue mold cheese, semi-hard cheese, and hard cheese. Typical examples of aged cheese include Gouda cheese, Edam cheese, Camembert cheese, and cheddar cheese.
As a yoghurt type material, it is obtained by the same manufacturing method as that of an existing yoghurt, or a material having the same quality (physical properties and flavor) as an existing yoghurt by adding an auxiliary material. Existing yogurt types include yogurts made in countries around the world, such as Bulgarian yogurt in Japan and Kefir yogurt in Russia.
(酸性大豆素材の食品への用途)
 本第二発明の酸性大豆素材は大豆の良好なコク味と酸性化(特に乳酸発酵)によるコク味が相俟って発酵乳製品などと同様のコクのある風味を有し、かつ後味のキレも良好であり、物性も滑らかなため、チーズやヨーグルト等の発酵乳製品と同様の各種食品用途に一部代替又は全部代替して使用することができる(発酵乳製品代替物)。各種食品へは配合のベースとなる主原料として、又は、練り込み用、フィリング用、トッピング用もしくはナッペ用の副原料として使用されうる。なお、主原料は食品の配合のベースとなる原料であり、限定はされないが概ね食品中に20重量%以上配合されるものをいう。また副原料は配合のベースとなる主原料に対し混合、注入、付着又は塗布する原料をいう。
(Use of acidic soybean material for food)
The acidic soybean material of the second invention has a rich flavor similar to that of fermented dairy products in combination with a good rich taste of soy and a rich taste due to acidification (particularly lactic acid fermentation), and has a sharp aftertaste. Is also good and the physical properties are smooth, so that it can be used partially or entirely for various food applications similar to fermented milk products such as cheese and yogurt (fermented milk product substitute). For various foods, it can be used as a main raw material as a base for blending, or as a secondary raw material for kneading, filling, topping or nappe. The main raw material is a raw material used as a basis for blending foods, but is not limited, but generally refers to those blended in foods by 20% by weight or more. The auxiliary raw material is a raw material to be mixed, injected, adhered or applied to the main raw material serving as a base for blending.
 例えば本酸性大豆素材をそのまま喫食用の製品として提供することができる。また、本酸性大豆素材に他の原料を配合して、フラワーペースト、スプレッド、フィリング、サワークリーム等の加工食品素材をさらに製造することができる。また、クリームソースやカレーソース等のソース類の原料として利用したり、菓子やパンの原料として直接配合して広く利用することができる。また、ちくわ等の水産練製品やハンバーグ等の畜肉製品のフィリング用やトッピング用としても利用することができる。 For example, this acidic soybean material can be provided as it is as a product for eating. Moreover, processed food materials, such as a flour paste, a spread, a filling, and a sour cream, can further be manufactured by mix | blending another raw material with this acidic soybean material. In addition, it can be used as a raw material for sauces such as cream sauce and curry sauce, or can be directly used as a raw material for confectionery and bread. It can also be used for filling and topping fishery products such as chikuwa and meat products such as hamburgers.
 菓子の分野では、洋菓子にも和菓子にも風味の相性が良好であり、特に洋菓子と和菓子の中でも生菓子が好ましい。洋生菓子としては、チーズケーキ、タルト、スポンジケーキ、パウンドケーキ、カステラ、バウムクーヘン、シュークリーム、ワッフル、クッキー、パイ、スフレ、ティラミス、プリン、ババロア等が挙げられ、和生菓子としては、もち類、団子類、ようかん、ういろう、どら焼き、たい焼き等が挙げられる。
 以下、菓子のより具体的な態様として、洋生菓子の例を示す。
In the field of confectionery, both confectionery and Japanese confectionery have good flavor compatibility, and among confectionery and Japanese confectionery, fresh confectionery is preferred. Western confectionery includes cheesecake, tarts, sponge cakes, pound cakes, castella, baumkuchen, cream puffs, waffles, cookies, pie, souffle, tiramisu, pudding, bavaria, and Japanese confectionery. , Yokan, Uiro, Dorayaki, Taiyaki, etc.
Hereinafter, an example of a Western confectionery will be shown as a more specific embodiment of the confectionery.
○レアチーズケーキ様食品
 ホエー非分離タイプの酸性大豆素材をフィルドタイプのチーズ素材(例えば不二製油(株)製「フロマージュブランアソルティエ」など)の代わりに主原料として用い、レアチーズケーキ様の食品を作成することができる。
 該食品中に本酸性大豆素材を5~70重量%好ましくは20~60重量%配合することが好ましい。その他の原料としては、レアチーズケーキに通常使用されるものを使用することができるが、例えば牛乳やクリーム等の乳原料、液糖やグラニュー糖などの糖類、メレンゲに使用する卵白、ゼラチン等のゲル化剤等を使用することができる。また、純植物性の食品にする場合は、乳原料の代わりに豆乳、大豆粉、分離大豆蛋白や、これらの大豆原料から調製した大豆クリーム等を使用することができる。
 このレアチーズケーキ様食品は、チーズやフィルドタイプのチーズで作製したレアチーズケーキと遜色がない品質であり、風味はコクがあり、且つ大豆由来の爽やかさのある滑らかな食感を有するものである。
○ Rare cheesecake-like food Use whey non-separable acidic soy material as a main ingredient instead of filled-type cheese material (for example, “Fromage Blanc Assortier” manufactured by Fuji Oil Co., Ltd.) Can be created.
The acidic soybean material is preferably blended in the food in an amount of 5 to 70% by weight, preferably 20 to 60% by weight. As other raw materials, those usually used for rare cheesecakes can be used. For example, milk raw materials such as milk and cream, sugars such as liquid sugar and granulated sugar, gels such as egg white and gelatin used for meringue An agent or the like can be used. In addition, when making a pure vegetable food, soy milk, soybean powder, separated soybean protein, soybean cream prepared from these soybean ingredients, or the like can be used instead of the milk ingredients.
This rare cheesecake-like food has a quality comparable to that of a rare cheesecake made of cheese or filled type cheese, has a rich flavor, and has a smooth texture with a refreshing feel derived from soybeans.
○ベイクドケーキ様食品
 ホエー分離タイプの酸性大豆素材をフィルドタイプのチーズ素材(例えば不二製油(株)製「クレメフロマージュ」など)の代わりに主原料として用い、ベイクドチーズケーキ様の食品を作成することができる。
 該食品中に本酸性大豆素材を5~70重量%好ましくは20~60重量%配合することが好ましい。その他の原料としては、ベイクドチーズケーキに通常使用されるものを使用することができるが、例えばクリーム等の乳原料、グラニュー糖等の糖類、ホワイトチョコレート、全卵や卵黄等の卵類、コーンスターチ等の澱粉類等をチーズ生地に配合することができる。
 このベイクドチーズケーキ様食品は、チーズやフィルドタイプのチーズで作製したものと外観上遜色なく、コクのある風味を有する。また食感はホクホクとした芋のような新たな食感が付与され、通常のベイクドチーズとは違った新しいケーキを得ることができる。
○ Baked cake-like foods Use whey-separated acidic soy materials as the main ingredients instead of filled-type cheese materials (for example, “Crime Fromage” manufactured by Fuji Oil Co., Ltd.) to create baked cheesecake-like foods be able to.
The acidic soybean material is preferably blended in the food in an amount of 5 to 70% by weight, preferably 20 to 60% by weight. As other ingredients, those commonly used for baked cheesecake can be used, but for example, milk ingredients such as cream, sugars such as granulated sugar, white chocolate, eggs such as whole eggs and egg yolks, corn starch, etc. Can be blended with cheese dough.
This baked cheesecake-like food has a rich flavor that is not inferior in appearance to that produced with cheese or filled-type cheese. In addition, the texture is given a new texture such as a crumpled rice cake, and a new cake different from ordinary baked cheese can be obtained.
<第三発明の実施形態>
(卵黄代替組成物)
 本第三発明の卵黄代替組成物は、本大豆乳化組成物を含有することを特徴としており、各食品に配合される卵黄の一部又は全部を代替することができるものである。各食品中の卵黄の本第三発明で代替できる卵黄とは、生卵黄、殺菌卵黄、加塩卵黄、加糖卵黄、乾燥卵黄等である。
<Embodiment of Third Invention>
(Egg yolk alternative composition)
The egg yolk substitute composition of the third invention is characterized by containing the present soybean emulsified composition, and can substitute a part or all of the egg yolk blended in each food. Egg yolks that can be substituted for the egg yolk in each food by the present third invention are raw egg yolk, sterilized egg yolk, salted egg yolk, sweetened egg yolk, dried egg yolk, and the like.
 本第三発明の卵黄代替組成物は、本大豆乳化組成物に、糖類、デンプン、香辛料、酸化防止剤、油脂、凝固剤を添加することで、品質、物性を改良した卵黄代替組成物として使用することができる。これらの物質は、1種以上を併用することができる。
 また、本第三発明の卵黄代替組成物は、本大豆乳化組成物に蛋白質架橋酵素を作用させたり、本大豆乳化組成物に、糖類、デンプン、香辛料、酸化防止剤、油脂及び凝固剤の群から選ばれる1種以上を添加したものに蛋白質架橋酵素を作用させることで、品質、物性を改良した卵黄代替組成物として使用することができる。
The egg yolk substitute composition of the third invention is used as an egg yolk substitute composition improved in quality and physical properties by adding sugar, starch, spices, antioxidants, oils and fats, and coagulants to the soybean emulsified composition. can do. One or more of these substances can be used in combination.
In addition, the egg yolk substitute composition of the third aspect of the invention is a group of sugars, starches, spices, antioxidants, oils and fats and coagulants in which the soy emulsion composition is allowed to act on a protein cross-linking enzyme. It can be used as an egg yolk substitute composition with improved quality and physical properties by allowing a protein cross-linking enzyme to act on one added with at least one selected from
○糖類
 本第三発明の卵黄代替組成物は、本大豆乳化組成物に糖類を添加して、さらに風味を改善した卵黄代替組成物とすることができる。ここで糖類とは、少糖類およびオリゴ糖類であり、具体的には、メープルシロップ、はちみつ、黒糖、グラニュー糖、砂糖、カラメル、果糖、果糖ぶどう糖液糖、水飴、マルトース、マルトトリオース、トレハロース、マルチトース等が例示できる。これらの糖類は、1種以上を併用することができる。
 上記糖類の含有量は、卵黄代替組成物中、好ましくは0.5~50重量%、さらに好ましくは3~30重量%である。
-Sugars The egg yolk substitute composition of the third invention of the present invention can be made into an egg yolk substitute composition in which a sugar is added to the soybean emulsified composition to further improve the flavor. Here, saccharides are oligosaccharides and oligosaccharides, specifically, maple syrup, honey, brown sugar, granulated sugar, sugar, caramel, fructose, fructose glucose liquid sugar, starch syrup, maltose, maltotriose, trehalose, A multitose etc. can be illustrated. One or more of these saccharides can be used in combination.
The content of the saccharide is preferably 0.5 to 50% by weight, more preferably 3 to 30% by weight in the egg yolk substitute composition.
○香辛料
 本第三発明の卵黄代替組成物は、本大豆乳化組成物に香辛料を添加して、さらに風味を改善した卵黄代替組成物とすることができる。香辛料として、からし、ワサビ、タイム、バジル、アニスシード、クローブ、フェンネル、ジンジャー、シナモン、ペパー、ターメリック、パプリカ、フェヌグリークシード、ナツメグ、スターアニス、オレガノ、ローレル、マジョラム、ローズマリー、ペパーミント、タラゴン、ディルシード等が例示できる。これらの香辛料は、1種以上を併用することができる。
 上記香辛料の含有量は、卵黄代替組成物中、好ましくは0.001~20重量%、さらに好ましくは0.01~10重量%である。
Spices The egg yolk substitute composition of the third invention can be made into an egg yolk substitute composition in which a spice is added to the soybean emulsified composition to further improve the flavor. As spices, mustard, wasabi, thyme, basil, anise seed, clove, fennel, ginger, cinnamon, pepper, turmeric, paprika, fenugreek seed, nutmeg, star anise, oregano, laurel, marjoram, rosemary, peppermint, tarragon, Examples include dill seeds. One or more of these spices can be used in combination.
The content of the spice is preferably 0.001 to 20% by weight, more preferably 0.01 to 10% by weight in the egg yolk substitute composition.
○酸化防止剤
 本第三発明の卵黄代替組成物は、本大豆乳化組成物に酸化防止剤を添加して、酸化による風味劣化が抑制された卵黄代替組成物とすることができる。酸化防止剤として、トコフェロール、ヤマモモ抽出物、アスコルビン酸、カテキン類、茶抽出物、例えばクエン酸ナトリウムのようなキレート効果のある物質等が例示できる。これらの酸化防止剤は、1種以上を併用することができる。
 上記酸化防止剤の含有量は、卵黄代替組成物中、好ましくは0.001~20重量%、さらに好ましくは0.01~10重量%である。
Antioxidant The egg yolk substitute composition of the third invention of the present invention can be made into an egg yolk substitute composition in which an antioxidant is added to the soybean emulsified composition and flavor deterioration due to oxidation is suppressed. Examples of the antioxidant include tocopherol, bayberry extract, ascorbic acid, catechins, tea extract, a substance having a chelating effect such as sodium citrate, and the like. One or more of these antioxidants can be used in combination.
The content of the antioxidant is preferably 0.001 to 20% by weight, more preferably 0.01 to 10% by weight in the egg yolk substitute composition.
○油脂
 本第三発明の卵黄代替組成物は、本大豆乳化組成物に油脂を添加してボディ感の付与等の物性改良機能を付与した卵黄代替組成物とすることができる。油脂として、食用に適する油脂であればよく、例えば、大豆油、菜種油、コーン油、綿実油、オリーブ油、落花生油、米油、べに花油、ひまわり油、パーム油、パーム核油、ヤシ油、サル脂、マンゴ脂、乳脂等や、更にこれらの硬化油、分別油、エステル交換油等の物理的または化学的処理を施した油脂を使用することができる。これらの油脂は、1種以上を併用することができる。
 上記油脂の含有量は、卵黄代替組成物中、好ましくは0.5~50重量%、さらに好ましくは3~30重量%である。
○ Oils and Fats The egg yolk substitute composition of the third invention can be an egg yolk substitute composition obtained by adding fats and oils to the soybean emulsified composition and imparting a physical property improving function such as imparting a body feeling. The oil and fat may be any oil and fat suitable for edible use. For example, soybean oil, rapeseed oil, corn oil, cottonseed oil, olive oil, peanut oil, rice oil, benflower oil, sunflower oil, palm oil, palm kernel oil, coconut oil, monkey fat Oils and fats that have been subjected to physical or chemical treatment such as mango fat, milk fat, and the like, and further these hardened oil, fractionated oil, transesterified oil and the like can be used. These fats and oils can use 1 or more types together.
The content of the oil or fat is preferably 0.5 to 50% by weight, more preferably 3 to 30% by weight in the egg yolk substitute composition.
○デンプン
 本第三発明の卵黄代替組成物は、本大豆乳化組成物にデンプンを添加して、ボディ感の付与等の物性改良機能を付与した卵黄代替組成物とすることができる。デンプンとして、コーンスターチ、ワキシーコーンスターチ、小麦デンプン、米デンプン、馬鈴薯デンプン、タピオカデンプン、甘藷デンプン、サゴデンプン等を使用でき、さらには、これらの化工デンプンも使用できる。該化工デンプンとしては、例えば、酸化デンプン、酸処理デンプン、酵素処理デンプン、酢酸デンプン、リン酸デンプン、コハク酸デンプン、オクテニルコハク酸デンプン、ヒドロキシプロピルデンプン、架橋デンプン、湿熱処理デンプン等いずれも用いることができる。これらのデンプンは、1種以上を併用することができる。
 上記デンプンの含有量は、卵黄代替組成物中、好ましくは0.5~50重量%、さらに好ましくは3~30重量%である。
○ Starch The egg yolk substitute composition of the third invention of the present invention can be an egg yolk substitute composition obtained by adding starch to the present soybean emulsified composition and imparting a physical property improving function such as a body feeling. As starch, corn starch, waxy corn starch, wheat starch, rice starch, potato starch, tapioca starch, sweet potato starch, sago starch and the like can be used, and these modified starches can also be used. As the modified starch, for example, oxidized starch, acid-treated starch, enzyme-treated starch, starch acetate, phosphate starch, succinate starch, octenyl succinate starch, hydroxypropyl starch, cross-linked starch, wet heat-treated starch, etc. can be used. it can. One or more of these starches can be used in combination.
The content of the starch is preferably 0.5 to 50% by weight, more preferably 3 to 30% by weight in the egg yolk substitute composition.
○凝固剤
 本第三発明の卵黄代替組成物は、本大豆乳化組成物に凝固剤を添加して、卵黄様の凝固機能を付与した卵黄代替組成物とすることができる。凝固剤として、にがり、塩化カルシウム、塩化マグネシウム、グルコノデルタラクトン、硫酸カルシウム等が挙げられる。これらの凝固剤は、1種以上を併用することができる。
 上記凝固剤の含有量は、卵黄代替組成物中、好ましくは0.001~10重量%、さらに好ましくは0.01~5重量%である。
O Coagulant The egg yolk substitute composition of the third invention of the present invention can be an egg yolk substitute composition obtained by adding a coagulant to the soybean emulsified composition to give an egg yolk-like coagulation function. Examples of the coagulant include bittern, calcium chloride, magnesium chloride, glucono delta lactone, calcium sulfate and the like. One or more of these coagulants can be used in combination.
The content of the coagulant is preferably 0.001 to 10% by weight, more preferably 0.01 to 5% by weight in the egg yolk substitute composition.
○蛋白質架橋酵素
 本第三発明の卵黄代替組成物は、本大豆乳化組成物に蛋白質架橋酵素を作用させて、乳化力等の乳化性能を上げた卵黄代替組成物とすることができる。この高い乳化性能は、特に酸性下の乳化物を調製する際において発揮される。
 蛋白質架橋酵素として、蛋白質分子同士の架橋を触媒する酵素であれば特に限定されないが、例えばアミノ基が関与するグルタミン残基とリジン残基の縮合反応、アスパラギン残基とリジン残基の縮合反応等のε-アミノ基が関与する反応を触媒するものが挙げられる。また、アミノ基は関与しないが、システイン残基のチオール基同士の縮合反応を触媒するものも使用できる。具体的には、グルタミン残基とリジン残基の縮合を触媒するトランスグルタミナーゼ、チオール基同士を縮合しジスルフィド結合の形成を触媒するチオール・ジスルフィド交換酵素等が挙げられ、これらの酵素は1種以上を併用して使用することができる。
 これらの酵素の中でもトランスグルタミナーゼを使用することが好ましい。トランスグルタミナーゼの起源は特に限定されず、動物由来、微生物由来、植物由来のものをいずれも使用できる。また、精製した酵素を使用しても良いし、市販の製剤を使用することも可能である。
 蛋白質架橋酵素の使用量は、本大豆乳化組成物の固形分に対して、好ましくは0.1重量%以上、より好ましくは0.5重量%以上、さらにより好ましくは0.9重量%以上である。また、酵素量は多過ぎても効果に差が出ない場合があるため、好ましくは15重量%以下、より好ましくは10重量%以下が適当である。
 蛋白質架橋酵素の作用温度は、概ね10~80℃、好ましくは20~70℃、より好ましくは40~60℃が適当である。温度が低すぎると酵素反応が遅くなる場合があり、温度が高すぎると酵素の失活が促進される場合がある。
 蛋白質架橋酵素の作用時間は概ね0.01~120分、好ましくは1~60分が適当である。作用時間が短すぎると十分な反応効果が得られない場合があり、作用時間が長すぎても効果に差がでない。
-Protein cross-linking enzyme The egg yolk substitute composition of the third invention of the present invention can be made into an egg yolk substitute composition having improved emulsifying performance such as emulsifying power by acting a protein cross-linking enzyme on the soybean emulsified composition. This high emulsification performance is exerted particularly when preparing an acidic emulsion.
The protein cross-linking enzyme is not particularly limited as long as it is an enzyme that catalyzes cross-linking of protein molecules. For example, a condensation reaction of a glutamine residue and a lysine residue involving amino groups, a condensation reaction of an asparagine residue and a lysine residue, etc. And those that catalyze reactions involving the ε-amino group. Moreover, although an amino group is not concerned, what catalyzes the condensation reaction of the thiol groups of a cysteine residue can also be used. Specific examples include transglutaminase that catalyzes the condensation of glutamine and lysine residues, and thiol / disulfide exchange enzyme that condenses thiol groups and catalyzes the formation of disulfide bonds. Can be used in combination.
Of these enzymes, transglutaminase is preferably used. The origin of transglutaminase is not particularly limited, and any of those derived from animals, microorganisms, and plants can be used. A purified enzyme may be used, or a commercially available preparation may be used.
The amount of the protein cross-linking enzyme used is preferably 0.1% by weight or more, more preferably 0.5% by weight or more, and even more preferably 0.9% by weight or more based on the solid content of the present soybean emulsion composition. Moreover, since there may be no difference in the effect even if the amount of enzyme is too large, it is preferably 15% by weight or less, more preferably 10% by weight or less.
The working temperature of the protein cross-linking enzyme is generally 10 to 80 ° C, preferably 20 to 70 ° C, more preferably 40 to 60 ° C. If the temperature is too low, the enzyme reaction may be slow, and if the temperature is too high, inactivation of the enzyme may be promoted.
The action time of the protein cross-linking enzyme is generally 0.01 to 120 minutes, preferably 1 to 60 minutes. If the action time is too short, a sufficient reaction effect may not be obtained, and even if the action time is too long, there is no difference in effect.
○その他の原料
 本第三発明の卵黄代替組成物は、必要に応じて、乳化剤、増粘剤、ゲル化剤、蛋白質、無機塩、有機塩、着色料、香料、酸味料等を添加して使用することができる。
○ Other raw materials The egg yolk substitute composition of the third invention is added with an emulsifier, a thickener, a gelling agent, a protein, an inorganic salt, an organic salt, a colorant, a fragrance, an acidulant and the like as necessary. Can be used.
(卵黄代替食品)
 本大豆乳化組成物を含有する卵黄代替組成物は以下に記載するように様々な食品に使用することができる。本大豆乳化組成物を含有する卵黄代替組成物を配合することにより、コクがあり、大豆由来の青臭みが抑制された風味の良い卵黄代替食品が得られる。
 本大豆乳化組成物を含有する卵黄代替組成物の使用量は本大豆乳化組成物の風味的特徴が明確に出る量であれば特に制限はないが、概ね乾物換算で卵黄代替食品に
対し0.5重量%以上、好ましくは0.5~60重量%、最も好ましくは1~50重量%である。下限未満では、本大豆乳化組成物の風味的特徴が明確に出ないため、好ましくない。本第三発明の卵黄代替食品は、各食品を製造するのに用いられる一般的な製法を用いて製造することができる。なお、本第三発明の卵黄代替食品は、卵黄代替組成物の他、上述の糖類、デンプン、香辛料、酸化防止剤、油脂、凝固剤、乳化剤、増粘剤、ゲル化剤、蛋白質、無機塩、有機塩、着色料、香料、酸味料等を添加して製造することができるし、卵黄代替組成物に蛋白質架橋酵素を作用させて製造することができる。
 本第三発明の卵黄代替食品として、調味料、菓子類、ソース類、惣菜等が例示できる。
(Egg yolk alternative food)
The egg yolk substitute composition containing the present soybean emulsified composition can be used in various foods as described below. By blending an egg yolk substitute composition containing the present soybean emulsified composition, a flavorful egg yolk substitute food that is rich and has a suppressed blue odor derived from soybeans can be obtained.
The use amount of the egg yolk substitute composition containing the soybean emulsified composition is not particularly limited as long as the flavor characteristic of the soybean emulsion composition is clearly shown, but is generally 0.5 wt. % Or more, preferably 0.5 to 60% by weight, most preferably 1 to 50% by weight. If it is less than the lower limit, the flavor characteristics of the present soybean emulsified composition are not clearly obtained, and therefore it is not preferable. The egg yolk substitute food of the third invention can be produced by using a general production method used for producing each food. The egg yolk substitute food of the third invention includes the above-mentioned egg yolk substitute composition, sugars, starches, spices, antioxidants, fats and oils, coagulants, emulsifiers, thickeners, gelling agents, proteins, inorganic salts It can be produced by adding organic salts, coloring agents, fragrances, acidulants, etc., and can be produced by allowing a protein cross-linking enzyme to act on an egg yolk substitute composition.
Examples of the egg yolk substitute food of the third invention include seasonings, confectionery, sauces, sugar beet, and the like.
○調味料
 本発明における調味料として、例えば、ドレッシング類、たれ類、マヨネーズ類、乾燥調味料等が挙げられる。
 ドレッシング類として、半固体状ドレッシング、乳化液状ドレッシング、分離液状ドレッシングが例示できる。
 たれ類とは、煮焼きに用いる調味液であり、例えば焼き肉のたれ、焼き鳥のたれ、照り焼きのたれ、しゃぶしゃぶのたれ、肉、魚介類の煮物用たれ等を挙げることができる。
 マヨネーズ類として、マヨネーズ、マヨネーズ風調味料、マヨネーズ様調味料等が挙げられる。
 マヨネーズ類に関して、蛋白質架橋酵素を作用させた卵黄代替組成物を使用することにより、例えば70重量%以上の油分の系において、特に卵黄を100%代替した場合にも、良好な乳化状態を維持し、風味の良好なマヨネーズ類を調製することができる。
○ Seasoning Examples of the seasoning in the present invention include dressings, sauces, mayonnaise, and dry seasonings.
Examples of dressings include semi-solid dressings, emulsified liquid dressings, and separated liquid dressings.
Sauces are seasoning liquids used for boiling, and examples thereof include grilled meat sauce, grilled chicken sauce, teriyaki sauce, shabu-shabu sauce, meat, and seafood sauce.
Examples of mayonnaise include mayonnaise, mayonnaise-like seasonings, mayonnaise-like seasonings, and the like.
With regard to mayonnaise, by using an egg yolk substitute composition in which a protein cross-linking enzyme is allowed to act, for example, in an oily system of 70% by weight or more, even when yolk is substituted 100%, a good emulsified state is maintained. Mayonnaise with good flavor can be prepared.
○菓子類
 本発明における菓子類として、例えば、クリーム類、デザート類、焼き菓子等が挙げられる。
 クリーム類とは、油相と水相とを混合し乳化したものであり、例としてカスタードクリーム、アイスクリーム、ホイップクリームなどが挙げられる。
カスタードクリームに関して、本第三発明の卵黄代替組成物とホワイトチョコレートを併用することでカスタードクリーム様食品の調製も可能となる。本第三発明で使用するホワイトチョコレートには、コーティング用のホワイトチョコレートも含まれる。なお、本発明におけるチョコレートとは全国チョコレート業公正取引協議会、チョコレート利用食品公正取引協議会で規定されるチョコレート、準チョコレート、チョコレート利用食品をいう。
 本第三発明のホワイトチョコレートと卵黄代替組成物の配合割合は特に制限されず、概ね1:99~99:1(重量比)の割合であり、ホワイトチョコレートの融点等の物性に応じて適宜重量比を変えて調製することができる。
 デザート類として、プリン、ババロア、ブリュレ等が挙げられる。プリンとして、カスタードタイプのプリン等が挙げられる。カスタードタイプのプリンには、オーブンの中で蒸し焼きする焼きタイプとレトルト釜や蒸し器等で蒸気加熱する蒸しタイプが含まれる。
 焼き菓子として、スポンジケーキ、パウンドケーキ、シフォンケーキ、パンケーキ、ロールケーキ、カステラ、ドーナツ、マドレーヌ、ビスケット、クッキー、ブリオッシュ、マフィン、ワッフル、ブラウニー、スフレ等が挙げられる。
○ Confectionery Examples of the confectionery in the present invention include creams, desserts, baked confectionery, and the like.
Creams are those obtained by mixing and emulsifying an oil phase and an aqueous phase, and examples thereof include custard cream, ice cream, and whipped cream.
Regarding custard cream, custard cream-like food can be prepared by using the egg yolk substitute composition of the third invention and white chocolate in combination. The white chocolate used in the third invention includes white chocolate for coating. In addition, the chocolate in this invention means the chocolate prescribed | regulated by the national chocolate industry fair trade council, the chocolate use food fair trade council, the semi-chocolate, and the chocolate use food.
The blending ratio of the white chocolate of the third invention and the egg yolk substitute composition is not particularly limited, and is generally a ratio of 1:99 to 99: 1 (weight ratio), and is appropriately weighted according to physical properties such as the melting point of white chocolate. It can be prepared by changing the ratio.
Examples of desserts include pudding, bavaroa and brulee. Examples of pudding include custard type pudding. The custard type pudding includes a grill type that is steamed in an oven and a steam type that is steam heated in a retort kettle or steamer.
Examples of baked goods include sponge cake, pound cake, chiffon cake, pancake, roll cake, castella, donut, madeleine, biscuit, cookie, brioche, muffin, waffle, brownie, souffle and the like.
○ソース類
 本発明におけるソース類として、カルボナーラソース、クリームソース、クリームスープ、オランデーズソース、カスタードソース、タルタルソース等が挙げられ、上記で例示したとおりスープも含まれる。
Sauces Examples of the sauces in the present invention include carbonara sauce, cream sauce, cream soup, hollandaise sauce, custard sauce, tartar sauce and the like, and soup is also included as exemplified above.
○惣菜
 本第三発明における惣菜として、茶碗蒸し、卵豆腐、卵雑炊等が挙げられる。
○ Side dish As the side dish in the third invention, steamed rice cake, egg tofu, egg soup, etc. are mentioned.
<第四発明の実施形態>
(大豆加工食品)
 本第四発明の大豆加工食品は、本大豆乳化組成物が原料として使用されていることを特徴とするものである。好ましくは、豆乳や粉末状大豆蛋白等の大豆原料を使用して加工される大豆加工食品であって、大豆原料の一部又は全部が本大豆乳化組成物に置換されてなるものをいう。より具体的には豆腐類、湯葉、大豆調味料、油揚げ、がんもどき、厚揚げ、飲用豆乳などであり、これらに類する範疇のものも含まれる。
 大豆加工食品に配合される本大豆乳化組成物は、通常の原料である豆乳や粉末状大豆蛋白等の大豆原料の全部あるいは一部を置き換えて配合することができる。これによって、大豆加工食品の食感や風味を改良することができる。置換率は大豆加工品の種類にもよるが、豆乳等の大豆原料の10重量%以上とすることができ、15重量%以上が好ましく、20重量%以上がより好ましく、25重量%以上とすることもできる。さらに求める品質により50重量%以上、70重量%以上、90重量%以上、あるいは100重量%とすることも可能である。以下、具体的な大豆加工食品の態様について説明するが、大豆加工食品の種類は下記態様に限定されるものではない。
<Embodiment of Fourth Invention>
(Processed soy food)
The soybean processed food of the fourth invention is characterized in that the soybean emulsion composition is used as a raw material. Preferably, it is a processed soybean food processed using soybean raw materials such as soy milk and powdered soybean protein, wherein a part or all of the soybean raw material is replaced with the present soybean emulsified composition. More specifically, it is tofu, yuba, soybean seasoning, deep-fried tofu, cancer throbbing, deep-fried deep-fried, soy milk for drinking, and the like categories.
The present soybean emulsified composition to be blended with processed soybean foods can be blended by replacing all or part of soybean raw materials such as soy milk and powdered soy protein which are ordinary raw materials. Thereby, the texture and flavor of the processed soybean food can be improved. Although the substitution rate depends on the type of processed soybean products, it can be 10% by weight or more of soybean raw materials such as soy milk, preferably 15% by weight or more, more preferably 20% by weight or more, and 25% by weight or more. You can also. Further, depending on the desired quality, it may be 50% by weight, 70% by weight, 90% by weight or 100% by weight. Hereinafter, although the aspect of a specific processed soybean food is demonstrated, the kind of processed soybean food is not limited to the following aspect.
○豆腐類
 本大豆乳化組成物、あるいは本大豆乳化組成物と豆乳との混合物に凝固剤を加え、加熱することにより蛋白質を凝固させ、豆腐を製造することができる。豆腐の製造は公知の製法で行うことができ、特に困難なく製造が可能である。凝固剤としてはニガリ(塩化マグネシウム)、塩化カルシウム、硫酸カルシウム、GDLなどを使用することができる。凝固剤の添加量は特に限定されないが、例えば本大豆乳化組成物及び豆乳の乾物に対して0.1~5重量%、好ましくは0.3~3重量%とすることができる。豆腐の硬さは凝固剤の量を増減させて適宜調整することができ、よりしっかりした硬さの豆腐を製造したい場合は凝固剤の量を多めに添加し、より軟らかくクリーミーな豆腐を製造したい場合は凝固剤の量を少なめに添加すればよい。概して得られた豆腐は従来の豆腐に比べると非常にクリーミーであり、口の中でとろけるような口溶けの良い食感であると共に、青臭い大豆臭がなくコクのある風味を呈する。
 なお、豆腐類には一般の製造において添加されている種々の添加剤を添加することができる。例えば凝固剤以外に塩類、オリゴ糖、多糖類、澱粉類、タンパク質などを単独あるいは併用して添加することができる。
Tofu Tofu can be produced by adding a coagulant to the present soybean emulsified composition or a mixture of the present soybean emulsified composition and soy milk and coagulating the protein by heating. Tofu can be produced by a known production method and can be produced without any particular difficulty. As the coagulant, bittern (magnesium chloride), calcium chloride, calcium sulfate, GDL and the like can be used. The amount of the coagulant added is not particularly limited, but may be, for example, 0.1 to 5% by weight, preferably 0.3 to 3% by weight, based on the present soybean emulsified composition and soy milk dry matter. The tofu hardness can be adjusted as appropriate by increasing or decreasing the amount of coagulant. If you want to produce a firmer tofu, add a larger amount of coagulant to produce a softer and creamier tofu. In such a case, the coagulant may be added in a small amount. In general, the tofu obtained is very creamy compared to conventional tofu, has a mouthfeel that melts in the mouth, and has a blue-smelling soybean odor and a rich flavor.
In addition, the various additives currently added in general manufacture can be added to tofu. For example, in addition to the coagulant, salts, oligosaccharides, polysaccharides, starches, proteins and the like can be added alone or in combination.
○湯葉
 本大豆乳化組成物を単独で、あるいは豆乳との併用で原料として使用し、これを加熱又は凍結することにより、表面に膜状の蛋白質の変性物が生成するため、これを回収して湯葉を製造することができる。湯葉の製造法は公知の手段で行うことができる。
 得られる湯葉はすくいたての生湯葉(汲み上げ湯葉)のようなとろりとした食感であり、通常の湯葉よりもコクのある風味を有する。また、食感の改質を行う場合、塩類、多糖類、デンプン類、タンパク質などを単独あるいは併用して本大豆乳化組成物に添加し、湯葉を調製することができる。
○ Yuba This soybean emulsified composition is used alone or in combination with soy milk as a raw material, and this is heated or frozen to produce a membrane-like protein denatured product. Yuba can be manufactured. The manufacturing method of yuba can be performed by a known means.
The resulting yuba has a rich texture like freshly cooked yuba (pumped yuba) and has a richer flavor than normal yuba. In addition, when modifying the texture, salts, polysaccharides, starches, proteins and the like can be added to the present soybean emulsified composition alone or in combination to prepare yuba.
○大豆調味料
 本大豆乳化組成物を調味料の原料に配合し、大豆調味料を製造することができる。調味料としては、ポン酢、しょう油、マヨネーズ、ドレッシング、ソース、ケチャップ、タレ、だし等の他、即席麺等に添付される液体もしくは粉末の調味料等が挙げられる。
 本大豆乳化組成物は調味料中に例えば20~80重量%配合することができる。
Soy seasoning The soybean emulsified composition can be blended with the raw material of the seasoning to produce a soy seasoning. Examples of the seasoning include ponzu, soy sauce, mayonnaise, dressing, sauce, ketchup, sauce, dashi, etc., and liquid or powder seasonings attached to instant noodles.
The soybean emulsified composition can be blended in the seasoning, for example, 20 to 80% by weight.
○油揚げ・がんもどき・厚揚げ
 本大豆乳化組成物を原料の一部に使用し、公知の製造法により油揚げ、がんもどき又は厚揚げ等の大豆加工食品を調製することができる。これらの大豆加工食品は、伝統的な製法で豆腐類を原料生地としてもよいし、あるいは粉末状大豆蛋白、水及び油脂をカッター等で混練し、乳化させて得られる大豆蛋白ペーストを原料生地としてもよい。
 厚揚げの場合、本大豆乳化組成物を使用すると豆腐類にこれを使用した場合と同様の食感と風味を付与することができる。
 また油揚げやがんもどきに本大豆乳化組成物を使用した場合、同様に大豆のコクのある風味を付与するだけでなく、フライ調理した際の生地原料の膨化をより促進し、膨化率を向上させる効果を与えることができる。そして膨化率が向上することにより、調理後の調味液の吸液性も向上し、よりジューシーな食感を与えることができる。
○ Deep-fried / Cancered / Deep-fried Soy emulsified composition can be used as a part of the raw material, and processed soybean foods such as deep-fried, deep-fried or deep-fried can be prepared by a known production method. These processed soybean foods may use tofu as a raw material dough by a traditional manufacturing method, or a soybean protein paste obtained by kneading and emulsifying powdered soy protein, water and fat with a cutter etc. as a raw material dough Also good.
In the case of deep-fried food, if the present soybean emulsified composition is used, the same texture and flavor can be imparted to tofu as when it is used.
In addition, when this soy emulsion composition is used for deep-fried chicken or cancer, it not only gives a rich flavor of soybeans, but also promotes the expansion of the dough raw material when fried and improves the expansion rate The effect to make can be given. And the expansion rate improves, The liquid absorptivity of the seasoning liquid after cooking can also improve, and a more juicy food texture can be given.
○飲用豆乳
 飲用豆乳は、豆腐製品の製造用豆乳ではなく、近年広く一般消費者に飲用されるようになった豆乳を利用した飲料であり、本第四発明においてはその製品規格は問わず豆乳が使用される飲料全般を含む。飲用豆乳に本大豆乳化組成物を使用すると、大豆のコクやクリーミー感を付与することができる。
○ Drinking soymilk Drinking soymilk is not a soymilk for the production of tofu products, but a beverage that uses soymilk that has been widely consumed by general consumers in recent years. Includes all beverages used. If this soybean emulsified composition is used for drinking soymilk, the richness and creamy feeling of soybeans can be imparted.
○冷凍大豆加工食品
 本第四発明の大豆加工食品はオリゴ糖、多糖類、澱粉類、ゼラチンなどの公知の冷凍変性防止剤を添加することにより、凍結解凍後も凍結前と同様の食感を維持する、冷凍耐性を付与させた大豆加工食品とすることもできる。これらは例えば冷凍豆腐、冷凍湯葉、冷凍厚揚げ、冷凍がんもどき、冷凍油揚げなどの冷凍食品として製品化することができる。冷凍変性防止剤の添加量は豆腐類中0.1~10重量%が好ましい。
 例えば従来の冷凍豆腐は風味があっさりとして大豆風味が少なく、食感は硬く口残りしやすいものであったが、本第四発明によれば本大豆乳化組成物の使用により冷凍豆腐の場合でも豆腐類への使用効果と同様の効果が得られる。
○ Frozen processed soybean food The processed processed food of the fourth invention has the same texture after freezing and thawing as before by freezing by adding known anti-freezing agents such as oligosaccharides, polysaccharides, starches and gelatin. It can also be set as the processed soybean food provided with freeze resistance. These can be commercialized as frozen foods such as frozen tofu, frozen yuba, deep-fried deep-fried, frozen cancer, and deep-fried frozen oil. The addition amount of the freezing denaturation inhibitor is preferably 0.1 to 10% by weight in the tofu.
For example, the conventional frozen tofu has a light and light soybean flavor, and the texture is hard and easy to taste. The effect similar to the use effect to the kind is obtained.
○乾燥大豆加工食品
 本第四発明の大豆加工食品は最終的にフリーズドライ、スプレードライ、マイクロ波乾燥、オーブン乾燥、自然乾燥、減圧乾燥等の種々の手段により、乾燥豆腐、乾燥湯葉、乾燥大豆調味料、乾燥油揚げ、粉末豆乳等の乾燥大豆加工品とすることができる。
 本第四発明の大豆加工食品が油揚げである場合、フライ後の油揚げを各種調味料を含む調味液に浸漬するなどして調味してから乾燥を行い、即席用具材としても用いることができる。本第四発明の油揚げは本大豆乳化組成物の使用により膨らみが良いため、調味し、乾燥した後もそのボリュームを維持し、湯を注いで復元させる場合においても、そのボリュームと優れた復元性(湯戻り性)を有する。
○ Processed dried soybean food The processed soybean food of the fourth invention is finally dried tofu, dried yuba, dried soybean by various means such as freeze drying, spray drying, microwave drying, oven drying, natural drying, and vacuum drying. Dried soybean processed products such as seasonings, fried dried oil, and powdered soy milk can be obtained.
When the processed soybean food of the fourth aspect of the present invention is fried, the fried fried food is seasoned, for example, by immersing it in a seasoning liquid containing various seasonings, and can be used as an instant ingredient. The deep-fried fried food of the fourth invention has a good swelling due to the use of the present soybean emulsified composition, so that the volume and excellent restorability can be maintained even when seasoning and maintaining the volume after drying and pouring hot water to restore it. (Removable hot water).
<第五発明の実施形態>
 本大豆乳化組成物には、顕著な血清コレステロール低減作用及び腎機能改善作用が存在することが、本発明者により見出された。当該血清コレステロール低減作用と腎機能改善作用は、本大豆乳化組成物を適当量食することにより見られる。すなわち、本大豆乳化組成物は、血清コレステロール低減用組成物又は腎機能改善用組成物の作用本体として用いることができるものである。
 本第五発明の当該血清コレステロール低減組成物と腎機能改善用組成物における本大豆乳化組成物の含有量は1~100重量%であり、望ましくは50~100重量%であり、さらに望ましくは80~100重量%であり、最も望ましくは100重量%である。
<Embodiment of Fifth Invention>
It has been found by the present inventor that the present soybean emulsified composition has a remarkable effect of reducing serum cholesterol and improving renal function. The serum cholesterol-reducing action and renal function-improving action can be seen by eating an appropriate amount of the soybean emulsified composition. That is, the present soybean emulsified composition can be used as an action body of a serum cholesterol reducing composition or a renal function improving composition.
The content of the soybean emulsified composition in the serum cholesterol reducing composition and the renal function improving composition of the fifth invention is 1 to 100% by weight, preferably 50 to 100% by weight, and more preferably 80%. ˜100% by weight, most desirably 100% by weight.
 また、本第五発明の血清コレステロール低減用組成物又は腎機能改善用組成物を所定量食品に含有させることで、血清コレステロール低減作用又は腎機能改善作用を示す食品を製造することが可能である。このように、「血清コレステロール低減作用を示す食品」や「腎機能改善作用を示す食品」を容易に調製できるのは、本第五発明の血清コレステロール低減用組成物において、良好な風味と血清コレステロール低減効果をあわせ持つことにより始めて導き出されるものであり、本第五発明により初めて達成されるものである。また、本第五発明の血清コレステロール低減用組成物又は腎機能改善用組成物を用いることで、血清コレステロール低減作用又は腎機能改善作用を示す特定保健用食品や、特別用途食品(病者用食品、えん下困難者用食品)を調製することもできる。
 この当該血清コレステロール低減作用又は腎機能改善作用を示す食品を製造する方法は、本第五発明の「血清コレステロール低減用組成物」又は「腎機能改善用組成物」を含有させることに技術的特長を有するものである。
Moreover, it is possible to produce a food exhibiting an effect of reducing serum cholesterol or improving renal function by containing a predetermined amount of the composition for reducing serum cholesterol or the composition for improving renal function of the fifth invention. . As described above, the “food that exhibits a serum cholesterol-reducing action” and the “food that exhibits a renal function-improving action” can be easily prepared in the composition for reducing serum cholesterol of the fifth invention with good flavor and serum cholesterol. It is derived for the first time by having a reduction effect, and is achieved for the first time by the fifth invention. In addition, by using the composition for reducing serum cholesterol or the composition for improving renal function according to the fifth aspect of the invention, a food for specified health use or a special-purpose food (food for a sick person) that exhibits an action for reducing serum cholesterol or an action for improving kidney function. , Foods for people with difficulty in swallowing).
This method for producing a food exhibiting an action for reducing serum cholesterol or an action for improving renal function is technically characterized by containing the “composition for reducing serum cholesterol” or the “composition for improving kidney function” of the fifth invention. It is what has.
 以上、本第一~第五発明の実施形態について述べたが、これらは何れも上述した特定の新規な大豆乳化組成物を用いることを共通の技術的特徴としており、各技術的思想のうち任意の2以上の思想を融合させた形態とすることも無論可能である。すなわち、例えば本大豆乳化組成物は使用される食品において、乳代替組成物かつ卵黄代替組成物、乳代替組成物かつ血清コレステロールもしくは腎機能改善用組成物、卵黄代替組成物かつ血清コレステロールもしくは腎機能改善用組成物などとして機能させることができる。 As described above, the embodiments of the first to fifth inventions have been described. However, all of these embodiments have a common technical feature that the specific novel soy emulsion composition described above is used. Of course, it is possible to combine two or more ideas. That is, for example, in the food used, the soy emulsion composition is a milk substitute composition and egg yolk substitute composition, a milk substitute composition and a serum cholesterol or kidney function improving composition, an egg yolk substitute composition and serum cholesterol or kidney function. It can function as a composition for improvement.
 以下に本発明の実施例を記載する。なお、以下「%」、「部」は特に断りのない限り「重量%」、「重量部」を意味する。脂質の分析は特に断りがない限りクロロホルム/メタノール混合溶媒抽出法に準じて行ったものである。 Examples of the present invention will be described below. Hereinafter, “%” and “parts” mean “% by weight” and “parts by weight” unless otherwise specified. Analysis of lipids was performed according to the chloroform / methanol mixed solvent extraction method unless otherwise specified.
(製造例1) 大豆乳化組成物の調製1
 湿熱加熱処理によりNSI 59.4とした大豆粉3.5kgに対して4.5倍量、50℃の水を加えて懸濁液とし、保温しながら30分間攪拌し、水抽出した。このときのpHは6.7であった。3層分離方式の遠心分離を6,000×gにて連続的に行い、(1)浮上層・(2)中間層・(3)沈殿層に分離させた。そして浮上層と沈殿層を合わせた画分6.3kgを回収し、大豆乳化組成物Aを調製した。
(Production Example 1) Preparation of soybean emulsified composition 1
The suspension was made by adding 4.5 times the amount of water at 50 ° C. to 3.5 kg of soybean powder made into NSI 59.4 by wet heat treatment, stirred for 30 minutes while keeping warm, and extracted with water. The pH at this time was 6.7. Centrifugation of a three-layer separation method was continuously performed at 6,000 × g, and separated into (1) a floating layer, (2) an intermediate layer, and (3) a precipitation layer. And the fraction 6.3kg which combined the floating layer and the precipitation layer was collect | recovered, and the soybean emulsification composition A was prepared.
(製造例2) 大豆乳化組成物の調製2
 製造例1にて調製した大豆乳化組成物Aに対して0.5重量倍の加水を行い、さらに13MPaにて高圧ホモゲナイザーで均質化した後、該均質化液を蒸気直接吹き込み方式で142℃7秒間加熱処理し、連続式遠心分離機にて6,000×gにて不溶性の繊維質を分離除去し、上清画分を得、これを大豆乳化組成物Bとした。
(Production Example 2) Preparation of soybean emulsified composition 2
The soybean emulsified composition A prepared in Production Example 1 was added 0.5 times by weight and further homogenized with a high-pressure homogenizer at 13 MPa, and then the homogenized liquid was heated at 142 ° C. for 7 seconds by a steam direct blowing method. The insoluble fiber was separated and removed at 6,000 × g with a continuous centrifuge, and a supernatant fraction was obtained.
 製造例1,2で得られた大豆乳化組成物A,Bを分析用に一部凍結乾燥し、一般成分として乾物、並びに、乾物あたりの蛋白質(ケルダール法による)、脂質(クロロホルム/メタノール混合溶媒抽出法による)及び灰分を測定し、さらにSDS-PAGEによりリポキシゲナーゼ蛋白質含量、LPの含量の推定値としてLCI値の分析を行った。また比較として、原料に用いた大豆粉、及び、米国特許第6,548,102号公報の方法で製造されていると推定される市販の大豆乳化組成物「Soy Supreme Kreme」(サンオプタ社(SunOpta Grains and Foods Group)製、粉末タイプ)についても同様に分析を行った。各分析値を表2に示す。 The soybean emulsified compositions A and B obtained in Production Examples 1 and 2 were partially lyophilized for analysis, dried as general components, protein per dry matter (by Kjeldahl method), lipid (chloroform / methanol mixed solvent) (By an extraction method) and ash content were measured, and further, LCI value was analyzed as an estimated value of lipoxygenase protein content and LP content by SDS-PAGE. For comparison, the soy flour used as a raw material and a commercially available soybean emulsified composition “Soy Supreme Kreme” (SunOpta Grains and Foods Group, presumed to be produced by the method of US Pat. No. 6,548,102) ) And powder type) were similarly analyzed. Each analysis value is shown in Table 2.
(表2)
Figure JPOXMLDOC01-appb-I000003
(Table 2)
Figure JPOXMLDOC01-appb-I000003
 大豆乳化組成物A,Bは大豆粉と比べると蛋白質に対する脂質含量に富み、さらに蛋白質組成が大豆粉や市販の大豆乳化組成物とは大きく異なるものであった。すなわちLCI値が60以上という高値であることから、LPが濃縮されたものであり、リポキシゲナーゼ蛋白質も高い含量であった。このような組成を有する大豆乳化組成物は、従来の豆乳やオカラなどにはない新規な成分組成である。
 これらの組成物A,Bの風味も既存の豆乳、大豆が本来有する自然な美味しさが濃縮されており、従来の問題とされていた大豆の青臭味や収斂味、渋味等の不快味がなく、非常にコクのある風味を有するものであった。一方、市販大豆乳化組成物は組成物A,Bに比べるとLCI値が大豆粉よりも低く、またリポキシゲナーゼ蛋白質が低いためか、コク味が少なく、青草味や収斂味の強い風味であった。
Soy emulsified compositions A and B were rich in lipid content relative to protein compared to soybean flour, and the protein composition was significantly different from soybean flour and commercially available soybean emulsified compositions. That is, since the LCI value was as high as 60 or more, LP was concentrated, and the lipoxygenase protein content was also high. The soybean emulsified composition having such a composition is a novel component composition not found in conventional soymilk or okara.
The flavors of these compositions A and B are also concentrated on the natural taste inherent in existing soymilk and soybeans, and unpleasant tastes such as the blue odor, astringency, and astringency of soybeans, which were previously considered problems. No, it had a very rich flavor. On the other hand, the commercially available soybean emulsified composition had a lower LCI value than soybean flour and a lower lipoxygenase protein than the compositions A and B, and was less flavorful and a strong flavor of green grass and astringency.
<第一発明の具体例>
(実施例a1)ホイップクリームの調製
 製造例2で調製した大豆乳化組成物B 29.9%、硬化パーム核油(融点34℃)29.9%、果糖ぶどう糖液糖(FC;王子コーンスターチ株式会社製)1.4%、オリゴトース(三菱化学フーズ株式会社製)1.4%、水 37.0%、レシチン 0.2%、ショ糖脂肪酸エステル「リョートーシュガーエステルS-570」(三菱化学フーズ株式会社製) 0.1%、ショ糖脂肪酸エステル「リョートーシュガーエステルS-1670」(三菱化学フーズ株式会社製) 0.1%、ポリリン酸ナトリウム 0.015%を調合し、60℃、15分間予備乳化した。さらに、5Mpaで高圧均質化してから、5℃にて一晩冷却して大豆含有ホイップクリーム原液を調製した。本クリーム原液1kgに対し、グラニュー糖70gを添加し、ホバートミキサーによる高速攪拌にて2分30秒間ホイップした。得られた大豆含有ホイップクリームのオーバーランは159.4%であった。本大豆含有ホイップクリームは、冷涼感のある口溶けで、大豆特有の青臭みがなく、コク味がある総合評価の高いものであった。
<Specific examples of the first invention>
(Example a1) Preparation of whipped cream 29.9% soybean emulsified composition B prepared in Production Example 2, 29.9% hydrogenated palm kernel oil (melting point 34 ° C.), fructose glucose sugar (FC; Oji Cornstarch Co., Ltd.) 1.4%, Oligotose (Mitsubishi Chemical Foods) 1.4%, Water 37.0%, Lecithin 0.2%, Sucrose fatty acid ester “Ryoto Sugar Ester S-570” (Mitsubishi Chemical Foods) Co., Ltd.) 0.1%, sucrose fatty acid ester “Ryoto Sugar Ester S-1670” (manufactured by Mitsubishi Chemical Foods Co., Ltd.) 0.1%, sodium polyphosphate 0.015%, 60 ° C., 15 Pre-emulsified for minutes. Furthermore, the mixture was homogenized under high pressure at 5 Mpa, and then cooled at 5 ° C. overnight to prepare a soybean-containing whipped cream stock solution. To 1 kg of the cream stock solution, 70 g of granulated sugar was added and whipped for 2 minutes 30 seconds by high-speed stirring with a Hobart mixer. The overrun of the obtained soybean-containing whipped cream was 159.4%. This soy-containing whipped cream melted in the mouth with a cool sensation, had no blue odor characteristic of soybeans, and had a high overall evaluation with a rich taste.
(比較例a1)
 実施例a1の大豆乳化組成物Bを、市販の大豆乳化組成物「Soy Supreme Kreme」(サンオプタ社(SunOpta Grains and Foods Group)製、粉末タイプ)を水に分散して、乾物含量が大豆乳化組成物Bと同等の18.2%に調整した分散液に置換して、実施例1同様に大豆含有ホイップクリーム原液を調製した。本クリーム原液1kgに対し、グラニュー糖70gを添加しホバートミキサー高速攪拌にて2分50秒間ホイップした。得られた大豆含有ホイップクリームのオーバーランは155.0%であった。本大豆含有ホイップクリームは、冷涼感のある口溶けであったが、大豆特有の青臭みがやや強く、コク味も弱いもので総合評価は低いものであった。
(Comparative Example a1)
The soybean emulsified composition B of Example a1 was dispersed in water with a commercially available soybean emulsified composition “Soy Supreme Kreme” (manufactured by SunOpta Grains and Foods Group, powder type). A soy-containing whipped cream stock solution was prepared in the same manner as in Example 1 by substituting the dispersion liquid adjusted to 18.2% equivalent to the product B. To 1 kg of the cream stock solution, 70 g of granulated sugar was added and whipped for 2 minutes and 50 seconds by high-speed stirring with a Hobart mixer. The overrun of the obtained soybean-containing whipped cream was 155.0%. The soy-containing whipped cream melted in the mouth with a cool sensation, but the soy-specific blue odor was slightly strong and the body was weak and the overall evaluation was low.
(参考例a1)
 品温5℃の生クリーム(油脂分30%、高梨乳業株式会社製)1kgにグラニュー糖70gを添加しホバートミキサーによる高速攪拌にて1分30秒間ホイップした。得られたホイップクリームのオーバーランは140.0%であった。本ホイップクリームは、冷涼感のある口溶けで、乳味、コク味とも優れており、総合評価の高いものであった。
(Reference Example a1)
70 g of granulated sugar was added to 1 kg of fresh cream (oil and fat content 30%, manufactured by Takanashi Milk Industry Co., Ltd.) having a product temperature of 5 ° C., and whipped for 1 minute 30 seconds by high-speed stirring with a Hobart mixer. The overrun of the obtained whipped cream was 140.0%. This whipped cream melted in the mouth with a cool sensation, and was excellent in both milkiness and richness, and was highly evaluated.
 表3に実施例a1、比較例a1、参考例a1の風味評価結果を示した。なお、以下の各例に示す風味評価は、パネラー10名による官能評価であり、下記評価基準でのパネラー平均評価である。
(評価基準)
-:まったく感じない  ±:ほとんど感じない  +:感じる
++:やや強く感じる  +++:強く感じる
◎:コクが強く、青臭みがない
○:コクがあり、青臭みもほとんどない
△:コクが弱く、青臭みがある
×:コクがなく、青臭みが強い
Table 3 shows the flavor evaluation results of Example a1, Comparative Example a1, and Reference Example a1. In addition, the flavor evaluation shown in each following example is sensory evaluation by 10 panelists, and is panel average evaluation in the following evaluation criteria.
(Evaluation criteria)
-: Not felt at all ±: Not felt at all +: Feeling ++: Feeling slightly strong ++++: Feeling strong ◎: Strong and no blue odor ○: Rich and almost no blue odor △: Low in strength and blue There is a smell ×: There is no richness, strong blue odor
(表3)
Figure JPOXMLDOC01-appb-I000004
(Table 3)
Figure JPOXMLDOC01-appb-I000004
(実施例a2) アイスクリームの調製
 製造例2で調製した大豆乳化組成物B 50%、上白糖 11%、ハチミツ(サクラ印純粋ハチミツ)8%を混合、攪拌後、アイスクリーマーを用いて70℃から攪拌、冷却し、フリージングされたアイスクリーム原液を得た。本原液69部に対し、実施例a1で得た大豆ホイップクリーム 31部を添加、混合して大豆アイスクリームを得た。本大豆アイスクリームを-20℃、15日保存後風味評価したところ、冷涼感のある軽い食感の口当たりで、大豆特有の青臭みがなく、コク味がある総合評価の高いものであった。
(Example a2) Preparation of ice cream 50% of the soybean emulsified composition B prepared in Production Example 2, 11% of super white sugar, and 8% of honey (sakura pure honey) were mixed, stirred, and then stirred at 70 ° C using an ice creamer. The mixture was stirred and cooled to obtain a frozen ice cream stock solution. To 69 parts of this stock solution, 31 parts of the soybean whipped cream obtained in Example a1 were added and mixed to obtain a soy ice cream. When this soy ice cream was stored at −20 ° C. for 15 days and evaluated for flavor, it was found to have a cool and light texture, no blue odor peculiar to soybeans, and a high overall evaluation with a rich taste.
(実施例a3)
 製造例2で調製した大豆乳化組成物B 50%、上白糖 11%、黒砂糖 7%、水 1%を混合、攪拌後、アイスクリーマーを用いて70℃から攪拌、冷却し、フリージングされたアイスクリーム原液を得た。本原液69部に対し、実施例a1で得た大豆ホイップクリーム 31部を添加、混合して大豆アイスクリームを得た。本大豆アイスクリームを-20℃、15日保存後風味評価したところ、冷涼感のある軽い食感の口当たりで、大豆特有の青臭みがなく、コク味がある総合評価の高いものであった。
(Example a3)
50% soy emulsified composition B prepared in Production Example 2, 11% super white sugar, 7% brown sugar, 1% water were mixed, stirred, and then stirred and cooled from 70 ° C. using an ice creamer, and then frozen. A cream stock solution was obtained. To 69 parts of this stock solution, 31 parts of the soybean whipped cream obtained in Example a1 were added and mixed to obtain a soy ice cream. When this soy ice cream was stored at −20 ° C. for 15 days and evaluated for flavor, it was found to have a cool and light texture, no blue odor peculiar to soybeans, and a high overall evaluation with a rich taste.
(比較例a2)
 実施例a2の大豆乳化組成物Bを、市販の大豆乳化組成物「Soy Supreme Kreme」(サンオプタ社(SunOpta Grains and Foods Group)製、粉末タイプ)を水に分散して、乾物含量が大豆乳化組成物Bと同等の18.2%に調整した分散液に置換して、実施例a2同様にフリージングされたアイスクリーム原液を得た。本原液69部に対し、比較例a1で得た大豆ホイップクリーム 31部を添加、混合して大豆アイスクリームを得た。本大豆アイスクリームを-20℃、15日保存後風味評価したところ、冷涼感のある軽い食感の口当たりであったが、大豆特有の青臭みがやや強く、コク味も弱いもので総合評価は低いものであった。
(Comparative Example a2)
The soybean emulsified composition B of Example a2 was dispersed in water with a commercially available soybean emulsified composition “Soy Supreme Kreme” (manufactured by SunOpta Grains and Foods Group). The dispersion was adjusted to 18.2%, which was equivalent to product B, and a frozen ice cream stock solution was obtained in the same manner as in Example a2. Soybean ice cream was obtained by adding and mixing 31 parts of the soy whipped cream obtained in Comparative Example a1 to 69 parts of this stock solution. The flavor of this soy ice cream was evaluated after storage at −20 ° C. for 15 days. The taste was cool and light, but the soy-specific blue odor was slightly strong and the rich taste was weak. It was low.
(参考例a2)
 実施例a2の大豆乳化組成物Bを、参考例a1の生クリームに代えて、実施例a2同様にフリージングされたアイスクリーム原液を得た。本原液69部に対し、参考例a1で得たホイップクリーム 31部を添加、混合してアイスクリームを得た。本アイスクリームを-20℃、15日保存後風味評価したところ、冷涼感のある軽い食感の口当たりで、乳味、コク味とも優れており、総合評価の高いものであった。
(Reference Example a2)
The soy emulsion composition B of Example a2 was replaced with the fresh cream of Reference Example a1 to obtain a frozen ice cream stock solution as in Example a2. Ice cream was obtained by adding and mixing 31 parts of the whipped cream obtained in Reference Example a1 to 69 parts of the stock solution. When this ice cream was evaluated for flavor after storage at −20 ° C. for 15 days, it had a cool, light texture and excellent milkiness and richness, and was highly evaluated.
 表4に実施例a2~a3、比較例a2、参考例a2の風味評価結果を示した。 Table 4 shows the flavor evaluation results of Examples a2 to a3, Comparative Example a2, and Reference Example a2.
(表4)
Figure JPOXMLDOC01-appb-I000005
(Table 4)
Figure JPOXMLDOC01-appb-I000005
(実施例a4) クリームスープの調製
 スイートコーン 30.1%、ローストオニオン(エバラ株式会社製)6.0%、製造例1で調製した大豆乳化組成物A 15.0%、上白糖3.0%、食塩0.3%、コンソメチキン味粉末0.5%、重曹 0.1%、水45.1%、白胡椒 適量を混合し、湯煎で85℃まで加熱して豆乳仕立てのコーンクリームスープを調製した。本スープは、とろみのあるなめらかな食感で、大豆特有の青臭みがなく、コク味がある総合評価の高いものであった。
(Example a4) Preparation of cream soup Sweet corn 30.1%, roasted onion (manufactured by Ebara Co., Ltd.) 6.0%, soybean emulsified composition A prepared in Production Example 1 15.0%, upper white sugar 3.0 %, Salt 0.3%, consomme chicken flavor powder 0.5%, baking soda 0.1%, water 45.1%, white pepper Was prepared. This soup had a thick and smooth texture, no blue odor characteristic of soybeans, and a high overall evaluation with a rich taste.
(比較例a3)
 実施例a4の大豆乳化組成物Aを、無調整豆乳(固形分6.5%、不二製油株式会社製)に代えて、実施例a4同様に豆乳仕立てのコーンクリームスープを調製した。本スープは、とろみのあるなめらかな食感であり、大豆特有の青臭みもほとんど感じられなかったが、コク味が弱いものであった。
(Comparative Example a3)
Soy emulsified composition A of Example a4 was replaced with non-adjusted soymilk (solid content 6.5%, manufactured by Fuji Oil Co., Ltd.) to prepare a corn cream soup tailored with soymilk in the same manner as Example a4. This soup had a thick and smooth texture and almost no soy-like blue odor, but had a weak body.
(参考例a3)
 実施例a4の大豆乳化組成物Aを参考例a1の生クリームに代えて、実施例a4同様にコーンクリームスープを調製した。本スープは、とろみのあるなめらかな食感で、乳味、コク味がある総合評価の高いものであった。
(Reference Example a3)
A corn cream soup was prepared in the same manner as in Example a4, except that the soybean emulsified composition A in Example a4 was replaced with the fresh cream in Reference Example a1. This soup had a thick and smooth texture and a high overall evaluation with milky and rich taste.
 表5に実施例a4、比較例a3、参考例a3の風味評価結果を示した。 Table 5 shows the flavor evaluation results of Example a4, Comparative Example a3, and Reference Example a3.
(表5)
Figure JPOXMLDOC01-appb-I000006
(Table 5)
Figure JPOXMLDOC01-appb-I000006
(実施例a5) ミルクプリン様の大豆由来原料含有飲食品
 製造例2で調製した大豆乳化物B 90.81%に対し、砂糖 9.08%、豆腐用のにがり(41ブリックス)0.11%を添加、混合してからカップ容器に充填し、95℃、25分間蒸してミルクプリン様の大豆由来原料含有飲食品を得た。本大豆由来原料含有飲食品は、やや弾力のあるなめらかなプリン様の食感で、大豆特有の青臭みがなく、コク味がある総合評価の高いものであった。
(Example a5) Milk pudding-like raw material-containing food and beverage containing soybeans 90.81% of the soybean emulsion B prepared in Production Example 2, 9.08% sugar, bittern for tofu (41 Brix) 0.11% Were added and mixed, and then filled into a cup container and steamed at 95 ° C. for 25 minutes to obtain a milk pudding-like raw material containing soybean-derived material. This soy-derived raw material-containing food and drink had a slightly supple and smooth pudding-like texture, no blue odor peculiar to soybeans, and a high overall evaluation with a rich taste.
(実施例a6) フルーツミルク様大豆飲料の調製
 水68.3%に砂糖 9.0%を溶解し、本溶液に対し攪拌しながら製造例2で調製した大豆乳化物B17.5%、2倍濃縮バナナ果汁 5.0%を添加、混合した。次いで、50%クエン酸水溶液を添加してPH6.0に調整した。次に、70℃まで加熱し15Mpaにて高圧均質化してから93℃まで加熱してバナナフレーバー 0.2%を添加し、冷水中で20℃まで冷却した。得られたバナナ風味飲料は、大豆特有の青臭みがなく、コク味がある総合評価の高いものであった。
(Example a6) Preparation of fruit milk-like soybean beverage 9.0% of sugar was dissolved in 68.3% of water, and 17.5% of the soybean emulsion B prepared in Production Example 2 was stirred while stirring the solution. Concentrated banana juice 5.0% was added and mixed. Subsequently, 50% citric acid aqueous solution was added to adjust the pH to 6.0. Next, the mixture was heated to 70 ° C., homogenized at a high pressure at 15 MPa, heated to 93 ° C., 0.2% of banana flavor was added, and the mixture was cooled to 20 ° C. in cold water. The obtained banana flavored drink had no blue smell peculiar to soybeans, and had a high overall evaluation with a rich taste.
(実施例a7) フルーツミルク様大豆飲料の調製
 水78.4%に砂糖9.0%、水溶性大豆多糖類「ソヤファイブS-ZR100」(不二製油株式会社製)の混合物を添加し、80℃で10分間攪拌、溶解した。本水溶液を20℃まで冷却し、製造例2で調製した大豆乳化物B 10.2%と5倍濃縮白桃透明果汁 2.0%を添加、混合した。次いで、50%クエン酸水溶液を添加してPH3.8に調整した。次に、70℃まで加熱し15Mpaにて高圧均質化してから93℃まで加熱してピーチフレーバー 0.1%を添加し、冷水中で20℃まで冷却した。得られたピーチ風味飲料は、大豆特有の青臭みがなく、コク味がある総合評価の高いものであった。
(Example a7) Preparation of a fruit milk-like soy beverage A mixture of 9.0% sugar and water-soluble soybean polysaccharide “Soya Five S-ZR100” (manufactured by Fuji Oil Co., Ltd.) was added to 78.4% water. The mixture was stirred and dissolved at 10 ° C. for 10 minutes. This aqueous solution was cooled to 20 ° C., and 10.2% soybean emulsion B prepared in Production Example 2 and 2.0% 5-fold concentrated white peach clear fruit juice were added and mixed. Subsequently, 50% citric acid aqueous solution was added to adjust the pH to 3.8. Next, the mixture was heated to 70 ° C., homogenized at a high pressure at 15 MPa, heated to 93 ° C., 0.1% of peach flavor was added, and the mixture was cooled to 20 ° C. in cold water. The obtained peach flavored beverage had no blue smell peculiar to soybeans and had a high overall evaluation with a rich taste.
(実施例a8) チーズスフレ様豆乳スフレの調製(1)
 ホバートミキサーボールに卵黄200gを入れグラニュー糖30gを添加し、中速攪拌で軽くホイップした。その後、低速攪拌しながら大豆乳化物B300g、薄力粉80gを順次添加した。最後に、別途、卵白220gに対しグラニュー糖100g、トレハロース(林原商事社製)70gを添加し、攪拌起泡させたメレンゲを添加、混合して比重0.29の豆乳スフレ生地を調製した。調製した生地600gを8取り鉄板に流し込み、あらかじめ水でぬらした新聞紙上に載置してオーブンに入れ、ダンパー開にて上火190℃、下火160℃で18分間焼成し、チーズスフレ様豆乳スフレを得た。得られたチーズスフレ様豆乳スフレは、チーズスフレ様のソフトな口当たりと良好な口溶けを有し、大豆特有の青臭みがなく、コク味がある総合評価の高いものであった。
(Example a8) Preparation of cheese souffle-like soy milk souffle (1)
In a Hobart mixer ball, 200 g of egg yolk was added, 30 g of granulated sugar was added, and lightly whipped with medium stirring. Thereafter, 300 g of soybean emulsion B and 80 g of weak flour were sequentially added while stirring at low speed. Finally, 100 g of granulated sugar and 70 g of trehalose (manufactured by Hayashibara Shoji Co., Ltd.) were added to 220 g of egg white, and a meringue foamed with stirring was added and mixed to prepare a soymilk souffle dough having a specific gravity of 0.29. 600 g of the prepared dough is poured into 8 iron plates, placed on newspaper wetted in advance, placed in an oven, baked for 18 minutes at 190 ° C and 160 ° C with an open flame, cheese souffle-like soy milk I got a souffle. The obtained cheese souffle-like soymilk souffle had a cheese souffle-like soft mouth feel and good mouth melt, no blue smell peculiar to soybeans, and a high overall evaluation with a rich taste.
(実施例a9) チーズスフレ様豆乳スフレの調製(2)
 ホバートミキサーボールに大豆乳化物B280gを入れ、低速攪拌しながら卵黄40g、グラニュー糖35g、コーンスターチ15gを順次添加混合した。さらに、低速攪拌しながらクリームチーズ様食品(商品名:クレメフロマージュ、不二製油株式会社製)320g、卵黄40g、薄力粉20gを添加、混合した。最後に、別途、卵白150gに対しグラニュー糖70g、トレハロース(林原商事社製)30gを添加し、攪拌起泡させたメレンゲを添加、混合して比重0.45の豆乳スフレ生地を調製した。調製した生地30gを直径50mmのアルミカップに流し込み、ダンパー開にて湯煎に入れた状態で上火180℃で30分間オーブン焼成した。得られたチーズスフレ様豆乳スフレは、チーズスフレ様のソフトな口当たりと良好な口溶けを有し、大豆特有の青臭みがなく、チーズ様風味とコク味がある総合評価の高いものであった。
(Example a9) Preparation of cheese souffle-like soymilk souffle (2)
280 g of soybean emulsion B was placed in a Hobart mixer ball, and 40 g of egg yolk, 35 g of granulated sugar, and 15 g of corn starch were sequentially added and mixed while stirring at a low speed. Furthermore, 320 g of cream cheese-like food (trade name: Creme Fromage, manufactured by Fuji Oil Co., Ltd.), egg yolk 40 g, and soft flour 20 g were added and mixed while stirring at low speed. Finally, 70 g of granulated sugar and 30 g of trehalose (manufactured by Hayashibara Shoji Co., Ltd.) were added to 150 g of egg white, and a meringue foamed with stirring was added and mixed to prepare a soymilk souffle dough having a specific gravity of 0.45. 30 g of the prepared dough was poured into an aluminum cup having a diameter of 50 mm, and baked in an oven at 180 ° C. for 30 minutes with the damper opened and placed in a hot water bath. The obtained cheese souffle-like soy milk souffle had a cheese souffle-like soft mouth feel and good mouth melt, no blue smell peculiar to soybeans, and a high overall evaluation with a cheese-like flavor and a rich taste.
<第二発明の具体例>
(実施例b1) 酸性大豆素材(ホエー非分離タイプ)の製造
 製造例2で得られた大豆乳化組成物Bを発酵前原料に用い、乳酸発酵してホエー非分離タイプの酸性大豆素材を下記の通り調製した。
 22℃に温調した大豆乳化組成物Bにクリスチャンハンセン社製チーズタイプ乳酸菌スターターを0.01重量%添加し、22℃にて8時間静置して発酵させた後、水酸化ナトリウムをpH5.6となるよう添加し、スパテラにて攪拌しつつ煮沸加熱し、70℃に達温するまで加熱殺菌を行った。
 得られた酸性大豆素材の風味と物性について専門パネラー5名を選定し、以降の実施例及び比較例も合わせて評価した。評価結果を表6に示した。本例の酸性大豆素材の風味は、雑味・不快味が少なくかつ発酵によるコク味を備えた良好なものであった。また性状はフレッシュチーズの一種であるフロマージュ・ブラン様の、ゆるいペースト状であった。
<Specific example of the second invention>
(Example b1) Production of acidic soybean material (whey non-separable type) The soybean emulsified composition B obtained in Production Example 2 was used as a raw material before fermentation, and lactic acid fermented to produce a non-whey segregating type acidic soy material as described below. Prepared as follows.
0.01% by weight of a cheese type lactic acid bacteria starter manufactured by Christian Hansen Co. was added to soybean emulsified composition B adjusted to 22 ° C., and left to stand at 22 ° C. for 8 hours for fermentation. The mixture was added to 6 and boiled while stirring with a spatula, and sterilized by heating until the temperature reached 70 ° C.
Five panelists were selected for the flavor and physical properties of the obtained acidic soybean material, and the following examples and comparative examples were also evaluated. The evaluation results are shown in Table 6. The flavor of the acidic soybean material of this example was good with little miscibility and unpleasant taste and with a rich taste by fermentation. In addition, it was a loose paste, like Fromage Blanc, a kind of fresh cheese.
(実施例b2) 酸性大豆素材(ホエー分離タイプ)の製造
 製造例2で得られた大豆乳化組成物Bを発酵前原料に用い、乳酸発酵してホエー分離タイプの酸性大豆素材を下記の通り調製した。
 実施例b1と同様にして、乳酸発酵、pH調整及び加熱殺菌を行った後、遠心分離機を用いてホエーを分離除去し、カードを回収した。得られた酸性大豆素材の風味は、クリームチーズ様の程良い固さを有し、雑味・不快味が少なくかつ発酵によるコク味を備えた良好なものであった。
(Example b2) Production of acidic soybean material (whey separation type) Using the soybean emulsified composition B obtained in Production Example 2 as a raw material before fermentation, lactic acid fermentation was performed to prepare a whey-separated type acidic soybean material as follows. did.
In the same manner as in Example b1, lactic acid fermentation, pH adjustment and heat sterilization were performed, and then whey was separated and removed using a centrifuge, and the card was collected. The flavor of the obtained acidic soybean material had a good hardness like cream cheese, had little miscibility and unpleasant taste, and had a good taste due to fermentation.
(実施例b3) 酸性大豆素材(ホエー分離タイプ、リン酸塩分離前添加)の製造
 実施例b2と同様にして乳酸発酵を行い、水酸化ナトリウムを用いたpH調整時にポリリン酸ナトリウムを0.2%添加し、再び実施例2と同様に加熱殺菌及びホエーの分離を行った。得られた酸性大豆素材の風味は、クリームチーズ様の程良い固さを有している上にざらつきが軽減され、雑味・不快味が少なくかつ発酵によるコク味を備えた良好なものであった。
(Example b3) Production of acidic soybean material (whey separation type, added before phosphate separation) Lactic acid fermentation was carried out in the same manner as in Example b2, and sodium polyphosphate was adjusted to 0.2 at the time of pH adjustment using sodium hydroxide. %, And heat sterilization and separation of whey were performed in the same manner as in Example 2. The flavor of the obtained acidic soy material has good hardness with cream cheese-like hardness, reduced roughness, less miscellaneous taste and unpleasant taste, and good taste with fermentation. It was.
(実施例b4) 酸性大豆素材(ホエー分離タイプ、リン酸塩分離後添加)の製造
 実施例b2と同様にして乳酸発酵、pH調整、加熱殺菌及びホエー分離を行った後、ニーダーにて混練しつつ少量の水に溶解させたポリリン酸ナトリウムを0.5%添加し、リン酸塩とタンパク質を反応させるために85℃まで昇温を行った。得られた酸性大豆素材の風味は、クリームチーズ様の程良い固さを有している上にざらつきが殆ど感じられず、雑味・不快味が少なくかつ発酵によるコク味を備えた良好なものであった。
(Example b4) Production of acidic soybean material (whey separation type, added after phosphate separation) After performing lactic acid fermentation, pH adjustment, heat sterilization and whey separation in the same manner as in Example b2, kneading with a kneader While adding 0.5% sodium polyphosphate dissolved in a small amount of water, the temperature was raised to 85 ° C. in order to react the phosphate with the protein. The flavor of the resulting acidic soy material is good with creamy cheese-like hardness, almost no roughness, little miscellaneous and unpleasant taste, and a rich taste from fermentation. Met.
(比較例b1) 分離大豆蛋白を原料とした酸性大豆素材
 分離大豆たん白「フジプロ-E」(不二製油(株)製)10%溶液を発酵原料に用い、実施例b1と同様にして、乳酸発酵、pH調整及び加熱殺菌を行った。得られた酸性大豆素材の風味は、舌を刺すようなざらつきを感じ、雑味・不快味が目立つ食しにくいものあった。
(Comparative Example b1) Acidic soybean material using isolated soybean protein as a raw material Separated soybean protein “Fuji Pro-E” (Fuji Oil Co., Ltd.) 10% solution was used as a fermentation raw material in the same manner as in Example b1, Lactic acid fermentation, pH adjustment and heat sterilization were performed. The flavor of the obtained acidic soy material was rough and ugly, and it was difficult to eat.
(比較例b2) 全脂豆乳を原料とした酸性大豆素材
 全脂豆乳「無調整豆乳」(不二製油(株)製)を発酵原料に用い、実施例b1と同様にして、乳酸発酵、pH調整及び加熱殺菌を行った。得られた酸性大豆素材の風味も、舌を刺すようなざらつきを感じ、雑味・不快味が際立っており、特に腐敗したような酸臭が目立つ食しにくいものあった。
(Comparative Example b2) Acid soy material made from full-fat soymilk Full-fat soymilk “Unregulated soymilk” (Fuji Oil Co., Ltd.) was used as a fermentation raw material in the same manner as in Example b1, lactic acid fermentation, pH Adjustment and heat sterilization were performed. The flavor of the obtained acidic soybean material was also rough and bitter and unpleasant.
(比較例b3) 市販大豆乳化組成物を原料とした酸性大豆素材
 市販大豆乳化組成物「Soy Supreme Kreme」(サンオプタ社(SunOpta Grains and Foods Group)製)を発酵原料に用い、加水して固形分を製造例2の大豆乳化組成物と同じにし、その他は実施例b1と同様にして、乳酸発酵、pH調整及び加熱殺菌を行った。得られた酸性大豆素材の風味も、舌を刺すようなざらつき・雑味・不快味が際立っており、特に腐敗したような酸臭が目立つ食しにくいものあった。
(Comparative Example b3) Acid soy material made from commercially available soybean emulsion composition Raw soybean emulsion composition “Soy Supreme Kreme” (manufactured by SunOpta Grains and Foods Group) is used as a fermentation raw material, and water is added to solid content. Was made the same as the soybean emulsified composition of Production Example 2, and lactic acid fermentation, pH adjustment and heat sterilization were performed in the same manner as in Example b1. The flavor of the obtained acidic soy material also had a rough, bitter, and unpleasant taste that pierced the tongue, and was particularly difficult to eat, with a noticeable rotten acid odor.
(表6)各種酸性大豆素材の風味及び食感評価
Figure JPOXMLDOC01-appb-I000007
(Table 6) Flavor and texture evaluation of various acidic soybean materials
Figure JPOXMLDOC01-appb-I000007
(応用例b1) レアチーズケーキ様食品
 実施例b1で得られた酸性大豆素材350部に対し、粉ゼラチン8部を水40部で膨潤させたものに、豆乳及び大豆粉の混合製品「ソイクレマ15」(不二製油(株)製)15部及び液糖15部を合わせ、加熱溶解したものを混合した。次いで、大豆クリーム「ソイクレマホイップ」(不二製油(株)製)360部をホイップ後に混合し、最後に卵白100部とグラニュー糖100部を混合して起泡させたメレンゲを混合し、比重約0.5のレアチーズケーキ様の食品を得た。
 得られたレアチーズケーキ様食品はチーズやフィルドタイプのチーズで作製したレアチーズケーキと遜色なく、コクがあり、且つ大豆由来の爽やかさのある滑らかな食感なものであった。
(Application example b1) Rare cheesecake-like food A soy milk and soy flour mixed product "Soy Crema 15" in which 350 parts of acidic soy material obtained in Example b1 is swollen with 8 parts of powdered gelatin in 40 parts of water 15 parts (Fuji Oil Co., Ltd.) and 15 parts of liquid sugar were combined and mixed by heating and dissolving. Next, 360 parts of soy cream “Soi Krema Whip” (Fuji Oil Co., Ltd.) was mixed after whipping, and finally 100 parts of egg white and 100 parts of granulated sugar were mixed together and mixed with meringue, and the specific gravity was mixed. About 0.5 rare cheesecake-like food was obtained.
The obtained rare cheesecake-like food was inferior to the rare cheesecake prepared with cheese or filled type cheese, had a rich body, and had a smooth texture with a refreshingness derived from soybeans.
(応用例b2) ベイクドチーズケーキ様食品
 実施例b3で得られた酸性大豆素材288部とグラニュー糖80部を混合したものに対し、クリーム「ガトーノイエ22」(乳脂肪分22%、不二製油(株)製)75部を加温してホワイトチョコレート「クーベルチュールホワイトカカオ」(不二製油(株)製)125部と合わせてしっかり乳化させたものを混合し、次いで全卵32.5部と卵黄32.5部を合わせたものを混合し、最後にコーンスターチ19.5部を混合して生地を作成した。
 次いでタルト生地「ドゥーフェリーデPS8H」 (不二製油(株)製)を上火160℃/下火160℃のオーブンで空焼きし、粉々に砕いたものに対しマーガリン「メサージュ500」(不二製油(株)製)を加熱溶解したものを混合した。次にこれを金属製のデコ型(直径12cm)に40g敷き詰めた。その上に酸性大豆素材で作製したチーズ生地を300g流し込み、40℃前後の湯を入れた天板にデコ型を乗せ、上火165℃/下火165℃のオーブンで30分前後焼成し、最後に上火を190℃に上げ5分間焼成し、焼き色が綺麗なベイクドチーズ様の食品を得た。得られたベイクドチーズケーキ様食品は、チーズやフィルドタイプのチーズで作製したものと見た目に遜色なく、コクがあるものになった。食感についてはホクホクとした芋のような新たな食感が付与され、通常のベイクドチーズとは違った新しいケーキを得ることができた。
(Application example b2) Baked cheesecake-like food A mixture of 288 parts of acidic soy material obtained in Example b3 and 80 parts of granulated sugar, cream "Gato Neue 22" (milk fat 22%, Fuji Oil ( Co., Ltd.) 75 parts heated and mixed with 125 parts white chocolate “Cuberture White Cacao” (Fuji Oil Co., Ltd.) and thoroughly emulsified, then mixed with 32.5 parts whole egg A mixture of 32.5 parts of egg yolk was mixed, and finally, 19.5 parts of corn starch were mixed to prepare a dough.
Next, the dough ferry de PS8H (manufactured by Fuji Oil Co., Ltd.) was baked in an oven at 160 ° C and 160 ° C, and margarine "Message 500" (Fuji) What was made by heating and dissolving (Oil Manufacturing Co., Ltd.) was mixed. Next, 40 g of this was spread on a metal deco mold (diameter 12 cm). Pour 300g of cheese dough made of acidic soybean material on top of it, place the deco mold on a top plate with hot water at around 40 ° C, and bake around 30 minutes in an oven at 165 ° C on top and 165 ° C on bottom. The top heat was raised to 190 ° C and baked for 5 minutes to obtain a baked cheese-like food with a beautiful baked color. The obtained baked cheesecake-like food was visually inferior to that produced with cheese or filled type cheese, and became rich. With regard to the texture, a new texture like that of a boiled rice cake was given, and a new cake different from ordinary baked cheese could be obtained.
<第三発明の具体例>
(実施例c1~c2、比較例c1) マヨネーズへの応用
 下記の表7に示した配合表に従って、米酢、油以外の原料を混合してホモミキサーで撹拌し、そこに米酢を加えてさらに撹拌を続けながら油を数回に分けて添加し、充分撹拌し、製造例2で得られた大豆乳化組成物Bを配合したマヨネーズを調製した。対照の配合は、マヨネーズの配合として一般的に用いられる配合をベースとした。なお、実施例c1、c2、比較例c1における卵黄代替率はそれぞれ、50、70、53%であった。
<Specific example of the third invention>
(Examples c1 to c2, comparative example c1) Application to mayonnaise According to the formulation shown in Table 7 below, ingredients other than rice vinegar and oil were mixed and stirred with a homomixer, and rice vinegar was added thereto. Further, the oil was added in several portions while continuing to stir, and stirred sufficiently to prepare mayonnaise blended with the soybean emulsified composition B obtained in Production Example 2. The control formulation was based on a formulation commonly used as a mayonnaise formulation. In addition, the yolk substitution rates in Examples c1 and c2 and Comparative Example c1 were 50, 70, and 53%, respectively.
(表7)マヨネーズ配合(%)
Figure JPOXMLDOC01-appb-I000008
(Table 7) Mayonnaise (%)
Figure JPOXMLDOC01-appb-I000008
 各配合で調製したマヨネーズについて、粘度、食感、風味の評価を行った結果を表8に示した。
 卵黄の50~70%を大豆乳化組成物Bで置換した実施例c1、c2のマヨネーズは、対照である市販のマヨネーズと同様の粘性を有し、マヨネーズ様の食感が得られた。また、大豆の青臭みがなく、クリーミーでかつコク味があり、風味は良好だった。
 一方、卵黄の53%を無調整豆乳で置換した比較例c1のマヨネーズは、粘度が低下し、マヨネーズ様の食感が得られなかった。また、大豆の青臭みはほとんど感じられないものの、コク味がなく、風味があっさりとしすぎていて良くなかった。
Table 8 shows the results of evaluation of the viscosity, texture and flavor of the mayonnaise prepared in each formulation.
The mayonnaise of Examples c1 and c2 in which 50 to 70% of the egg yolk was replaced with the soybean emulsified composition B had the same viscosity as that of the commercially available mayonnaise as a control, and a mayonnaise-like texture was obtained. Moreover, there was no blue smell of soybean, it was creamy and rich, and the flavor was good.
On the other hand, the mayonnaise of Comparative Example c1 in which 53% of egg yolk was replaced with unadjusted soymilk had a reduced viscosity and a mayonnaise-like texture could not be obtained. Moreover, although the blue smell of soybean was hardly felt, it was not good because there was no richness and the flavor was too light.
(表8)
Figure JPOXMLDOC01-appb-I000009
(Table 8)
Figure JPOXMLDOC01-appb-I000009
(実施例c3) ドレッシングへの応用
 水280gに水溶性大豆多糖類(ソヤファイブ-S;不二製油(株)製)3gを添加し攪拌しながら溶解した。次いで砂糖70g、醤油70g、食塩10g、食酢160g、鰹節エキス7g、を添加する。よく攪拌混合した後、製造例2で得られた大豆乳化組成物B 400gを徐々に添加し全量を1000gとした。その後更にホモミキサーで均質化し、90℃まで加熱して容器に充填しドレッシングを得た。
 得られたドレッシングは、コク味があり、良好な風味であった。
(Example c3) Application to dressing 3 g of water-soluble soybean polysaccharide (Soya Five-S; manufactured by Fuji Oil Co., Ltd.) was added to 280 g of water and dissolved while stirring. Next, 70 g of sugar, 70 g of soy sauce, 10 g of salt, 160 g of vinegar, and 7 g of bonito extract are added. After thoroughly stirring and mixing, 400 g of the soybean emulsified composition B obtained in Production Example 2 was gradually added to make the total amount 1000 g. Thereafter, the mixture was further homogenized with a homomixer, heated to 90 ° C., filled into a container, and a dressing was obtained.
The obtained dressing had a rich taste and a good flavor.
(実施例c4) たれへの応用
 鍋にみりん100gを入れ火にかけて煮沸し、煮切りみりんとした。次いで水500gと醤油150gを加え、煮立ってきたら削り節20gを加えて暫く煮詰めた。その後火を止めて室温で冷却し、ガーゼで削り節を漉し取り、つゆ部を調製した。
 上記の方法で得た、つゆ部300gと製造例1で得られた大豆乳化組成物A 700gをホモミキサーを用いて均質化し、90℃まで加熱後容器に充填してたれを得た。
 得られたたれは滑らかな食感でコク味があり、良好な風味であった。
(Example c4) Application to sauce 100 g of mirin was placed in a pan and boiled over fire to make boiled mirin. Next, 500 g of water and 150 g of soy sauce were added, and when boiled, 20 g of shavings were added and simmered for a while. Thereafter, the fire was stopped and the mixture was cooled at room temperature. The shavings were scraped off with gauze to prepare a soup portion.
300 g of the soup part obtained by the above method and 700 g of the soybean emulsified composition A obtained in Production Example 1 were homogenized using a homomixer, heated to 90 ° C., and filled into a container to obtain a sauce.
The obtained sauce had a smooth texture and a rich taste, and had a good flavor.
(実施例c5) カスタードクリーム様食品(メープルシロップ配合)への応用
 製造例2で得られた大豆乳化組成物B 200g、薄力粉15g、果糖ぶどう糖液糖(FC;王子コーンスターチ(株)製)15g、上白糖30g、オリゴトース(三菱化学フーズ(株)製)5g、メープルシロップ(メープルシロップNo1 ミディアム;共立食品(株)製)10g、水45gを混合後、一度濾した。濾したものを鍋に入れ加熱し、沸騰後5分間練り、蒸発した水分量の分だけ水を添加して混合し、メープルシロップを配合したカスタードクリーム様食品を調製した。
 得られた、カスタードクリーム様食品は滑らかな食感で、コク味があり、大豆の青臭みが感じられず、良好な風味であった。
(Example c5) Application to custard cream-like food (containing maple syrup) Soy emulsified composition B 200 g obtained in Production Example 2, 15 g of weak flour, fructose-glucose liquid sugar (FC; manufactured by Oji Cornstarch Co., Ltd.), After mixing 30 g of super white sugar, 5 g of oligotose (manufactured by Mitsubishi Chemical Foods), 10 g of maple syrup (maple syrup No. 1 medium; manufactured by Kyoritsu Foods), 45 g of water were filtered once. The filtered product was put in a pan, heated, kneaded for 5 minutes after boiling, and water was added and mixed for the amount of evaporated water to prepare a custard cream-like food containing maple syrup.
The obtained custard cream-like food had a smooth texture, had a rich taste, did not feel the blue odor of soybeans, and had a good flavor.
(比較例c2)
 大豆乳化組成物Bの代わりに、「Soy Supreme Kreme」(サンオプタ社(SunOpta Grains and Foods Group)製、粉末タイプ)を水と混合して固形分含量を18.2%にしたものを200g使用した以外は、実施例c5と同様にしてカスタードクリーム様食品を調製した。
 得られたカスタードクリーム様食品はコク味がやや不足しており、また、大豆の青臭みが感じられ風味として好ましいものではなかった。
(Comparative Example c2)
Instead of soy emulsified composition B, 200g of "Soy Supreme Kreme" (manufactured by SunOpta Grains and Foods Group, powder type) mixed with water to make the solid content 18.2% was used. A custard cream-like food was prepared in the same manner as in Example c5.
The obtained custard cream-like food was slightly lacking in richness, and a blue odor of soybean was felt, which was not preferable as a flavor.
(実施例c6) カスタードクリーム様食品(黒糖配合)への応用
 製造例2で得られた大豆乳化組成物B 200g、薄力粉15g、果糖ぶどう糖液糖(FC;王子コーンスターチ(株)製)15g、上白糖32g、オリゴトース(三菱化学フーズ(株)製)5g、黒砂糖(上野砂糖(株)製)5g、水48gを混合後、一度濾した。濾したものを鍋に入れ加熱し、沸騰後5分間練り、蒸発した水分量の分だけ水を添加して混合し、黒糖を配合したカスタードクリーム様食品を調製した。
 得られた、カスタードクリーム様食品は滑らかな食感で、コク味があり、良好な風味であった。
(Example c6) Application to custard cream-like food (brown sugar combination) 200 g of soybean emulsified composition B obtained in Production Example 2, 15 g of flour, 15 g of fructose glucose liquid sugar (FC; manufactured by Oji Cornstarch Co., Ltd.), upper After mixing 32 g of white sugar, 5 g of oligotose (manufactured by Mitsubishi Chemical Foods), 5 g of brown sugar (manufactured by Ueno Sugar Co., Ltd.) and 48 g of water, the mixture was filtered once. The filtered product was put in a pan, heated, kneaded for 5 minutes after boiling, and water was added and mixed for the amount of evaporated water to prepare a custard cream-like food containing brown sugar.
The obtained custard cream-like food had a smooth texture, a rich taste, and a good flavor.
(実施例c7) プリン様食品への応用
 全卵28g、砂糖14g、製造例2で得られた大豆乳化組成物B 58gを混合し、80℃で加熱後、オーブンで湯煎焼成(150℃)してプリン様食品を調製した。得られたプリン様食品は食感、風味とも良好であった。
(Example c7) Application to pudding-like food 28 g of whole egg, 14 g of sugar and 58 g of the soybean emulsified composition B obtained in Production Example 2 were mixed, heated at 80 ° C., and then baked in a hot water bath (150 ° C.). A pudding-like food was prepared. The obtained pudding-like food had good texture and flavor.
(実施例c8) ババロア様食品への応用
 製造例2で得られた大豆乳化組成物B 92g、砂糖7gを混合後80℃で加熱し、40℃に冷却後、あらかじめ少量の水で膨潤させたゼラチン1gを添加し、冷蔵庫で4時間冷却して固め、ババロア様食品を調製した。
 得られたババロア様食品は食感、風味とも良好であった。
(Example c8) Application to Bavaroa-like foods 92 g of soybean emulsified composition B obtained in Production Example 2 and 7 g of sugar were mixed, heated at 80 ° C., cooled to 40 ° C., and then swollen with a small amount of water in advance. 1 g of gelatin was added and cooled in a refrigerator for 4 hours to harden to prepare a bavaroa-like food.
The resulting Bavaroa-like food had good texture and flavor.
(実施例c9) ブリュレ様食品への応用
 製造例2で得られた大豆乳化組成物B 80g、砂糖8g、にがり(Brix=41)0.1gを混合後、蒸し器で95℃、25分蒸した後、カラメリゼ処理を行いブリュレ様食品を調製した。得られたブリュレ様食品は、適度な固さがあり、滑らかな食感で良好な風味であった。
(Example c9) Application to brulee-like food After mixing 80 g of soybean emulsified composition B obtained in Production Example 2 and 8 g of sugar and 0.1 g of bittern (Brix = 41), steamed at 95 ° C. for 25 minutes in a steamer. Then, caramelized treatment was performed to prepare a brulee-like food. The obtained brulee-like food had an appropriate hardness, a smooth texture and a good flavor.
(実施例c10) カルボナーラソースへの応用
 製造例1により得られた大豆乳化組成物A 52g、粉チーズ25g、ベーコン15g、ベーキングパウダー0.5gを混合して、カルボナーラソースを調製した。
 得られたカルボナーラソースは、コク味があり良好な風味であった。また、このソースを茹でたパスタと和えた場合も、パスタと和えやすく、良好な風味であった。
(Example c10) Application to carbonara sauce Carbonara sauce was prepared by mixing 52 g of soybean emulsified composition A obtained in Production Example 1, 25 g of powdered cheese, 15 g of bacon, and 0.5 g of baking powder.
The obtained carbonara sauce had a rich taste and a good flavor. Also, when this sauce was mixed with boiled pasta, it was easy to mix with pasta and had a good flavor.
(実施例c11) 茶碗蒸しへの応用
 製造例2で得られた大豆乳化組成物B 50g、卵25g、だし汁120g、食塩1gを混合後、型に入れ、蒸し器で85℃、15分で蒸し、茶碗蒸しを得た。得られた茶碗蒸しは適度な硬さで滑らかな食感であり、良好な風味であった。
(Example c11) Application to chawanmushi 50 g of soybean emulsified composition B obtained in Production Example 2, 25 g of egg, 120 g of soup stock and 1 g of salt are mixed, put into a mold, and steamed in a steamer at 85 ° C. for 15 minutes. Got. The resulting chawanmushi was moderately hard with a smooth texture and a good flavor.
(実施例c12) マヨネーズへの応用
 製造例2で得られた大豆乳化組成物Bにトランスグルタミナーゼ(商品名:アクティバ、味の素(株)製)を大豆乳化組成物Bに対して0.5%(大豆乳化組成物Bの固形分に対して2.7%)添加して、55℃、30分間反応させた後90℃、30分間加熱して反応を停止し、トランスグルタミナーゼを作用させた卵黄代替組成物を得た。
 この卵黄代替組成物40g、粉からし0.7g、食塩3g、グルタミン酸ナトリウム0.25g、りんご酢と酢酸を混合して酸度10%に調整したもの8g、水8gを混合し、これに菜種油140gをフードカッターで攪拌しながら添加してマヨネーズを調製した。このマヨネーズの油分は70%であった。
 このマヨネーズは卵黄を、卵黄代替組成物に100%代替して調製したものであるが、乳化状態は非常に良好で安定しており、大豆を酢漬けしたような特有の風味もなく、コク味があり風味も良好であった。
(Example c12) Application to mayonnaise Transglutaminase (trade name: Activa, manufactured by Ajinomoto Co., Inc.) was added to the soybean emulsion composition B obtained in Production Example 2 at 0.5% (soy emulsion). 2.7% based on the solid content of composition B), react at 55 ° C. for 30 minutes, and then stop at 90 ° C. for 30 minutes to stop the reaction and obtain an egg yolk substitute composition in which transglutaminase is allowed to act It was.
40 g of this egg yolk substitute composition, 0.7 g of powdered mash, 3 g of salt, 0.25 g of sodium glutamate, 8 g of apple vinegar and acetic acid mixed to adjust the acidity to 10%, 8 g of water are mixed, and 140 g of rapeseed oil is added to the food. Mayonnaise was prepared by adding with stirring with a cutter. The mayonnaise oil content was 70%.
This mayonnaise was prepared by substituting egg yolk for 100% with egg yolk substitute composition, but the emulsified state is very good and stable, there is no special flavor like pickled soybean, and the rich taste There was good flavor.
(実施例c13) カスタードクリーム様食品への応用
 ホワイトチョコレート(商品名:ホワイトコーチングYDV36、不二製油(株)製)4重量部を50℃の湯煎で溶解した。湯煎から外したホワイトチョコレートに、製造例2で得られた大豆乳化組成物B 3重量部を少量ずつ添加しながら静かに攪拌して混合し、カスタードクリーム様食品を調製した。
 得られたカスタードクリーム様食品は滑らかな食感でコク味があり、大豆の青臭みも感じられず良好な風味であった。
(Example c13) Application to custard cream-like food 4 parts by weight of white chocolate (trade name: White Coaching YDV36, manufactured by Fuji Oil Co., Ltd.) was dissolved in a hot water bath at 50 ° C. A custard cream-like food was prepared by adding 3 parts by weight of the soybean emulsified composition B obtained in Production Example 2 to the white chocolate removed from the hot water bath while gently stirring.
The obtained custard cream-like food had a smooth texture, a rich taste, and a good flavor without the blue smell of soybeans.
<第四発明の具体例>
(比較例d1) 豆腐(対照)
 市販の豆乳(乾物含量11%)80部に、にがり0.5部を添加し、良く混合して容器に充填し、95℃で25分間蒸し器で蒸煮し、自然冷却し、従来から製造されている伝統的な豆腐を得た。
 得られた豆腐は、硬く、もろいゲルであり、口の中ではすぐに溶けず、咀嚼することにより細かく砕けるような食感であった。
<Specific example of the fourth invention>
(Comparative Example d1) Tofu (control)
Traditionally produced traditionally manufactured by adding 0.5 parts of bittern to 80 parts of commercial soymilk (dry matter content 11%), mixing well, filling into a container, steaming in a steamer at 95 ° C for 25 minutes, naturally cooling Got a typical tofu.
The tofu obtained was a hard and fragile gel that did not melt immediately in the mouth, and had a texture that could be finely broken by chewing.
(実施例d1) 豆腐2
 製造例2で得られた大豆乳化組成物B(乾物含量18.2%)80部に、にがりを0.1部添加し、良く混合して、容器に充填し、95℃で25分間蒸し器で蒸煮し、自然冷却して豆腐を得た。
 得られた豆腐は、比較例d1の豆腐のように硬くてもろいゲル物性ではなく、軟らかくてさっくりとしたスプーン通りである物性であった。また食感はクリーミーでとろけるように口溶けが良いもので、豆腐というよりも、滑らかな食感のクリーム入りのプリン、あるいは、あんきも(アンコウの肝)のようなコクのある口溶けの良い食感であった。また風味は比較例d1の豆腐の風味とは明らかに異なるものであり、より濃厚かつ良好な大豆の風味を有していた。
 得られた本第四発明の豆腐にポン酢しょう油をかけると非常に美味であった。
(Example d1) Tofu 2
Add 80 parts of bittern to 80 parts of soybean emulsified composition B (dry matter content 18.2%) obtained in Production Example 2, mix well, fill into a container, cook in a steamer at 95 ° C for 25 minutes, Cooled to obtain tofu.
The obtained tofu was not as hard and brittle as the tofu of Comparative Example d1, but had a soft and crisp physical property. In addition, the mouthfeel is creamy and melts well in the mouth, rather than tofu, with a creamy pudding with a smooth mouthfeel, or a rich mouthfeel like Ankimo (ancho liver) there were. The flavor was clearly different from the tofu flavor of Comparative Example d1, and had a richer and better soybean flavor.
It was very delicious when ponzu soy sauce was applied to the obtained tofu of the fourth invention.
(実施例d2) 豆腐3
 比較例d1の配合において、豆乳8部、32部、48部をそれぞれ製造例2の大豆乳化組成物Bと置換し、同様の方法で豆乳の置換率が10%、40%、60%の各豆腐を得た。
 得られた豆腐は置換率が高いほどコクのある風味で、クリーミー感が付与された食感となった。
(Example d2) Tofu 3
In the formulation of Comparative Example d1, 8 parts, 32 parts, and 48 parts of soy milk were replaced with the soybean emulsified composition B of Production Example 2, and the substitution rate of soy milk was 10%, 40%, and 60%, respectively, in the same manner. Got tofu.
The obtained tofu had a richer flavor as the substitution rate was higher, and became a texture with a creamy feeling.
(実施例d3) 大豆調味料
 製造例2で得られた大豆乳化組成物B 75部に対してポン酢しょう油25部を加えてよく混合し、大豆調味料を得た。この調味料を刺身にかけて食したところ、クリーミーでまろやかな酸味とコクがあり、ゴマだれ様の外観色と粘度であり、刺身との風味の相性も非常に良いものであった。
(Example d3) Soy seasoning Soy emulsified composition B obtained in Production Example 2 was mixed with 75 parts of ponzu soy sauce and mixed well to obtain a soy seasoning. When this seasoning was eaten over sashimi, it had a creamy, mellow acidity and richness, an appearance color and viscosity similar to sesame seeds, and a very good flavor compatibility with sashimi.
(実施例d4) 湯葉
 製造例2で得られた大豆乳化組成物Bを平板状のトレーに高さ2cmまで満たし、-20℃の凍結庫で10日間凍結した。これを解凍したところ、細かい湯葉片となり、これを食すると、とろみのある食感で、くみ上げ湯葉様の食感と風味になった。これに調味液を加えるとさらに美味しく食すことができた。
(Example d4) Yuba The soybean emulsion composition B obtained in Production Example 2 was filled in a flat tray to a height of 2 cm and frozen in a freezer at −20 ° C. for 10 days. When this was thawed, it became a fine yuba piece, and when it was eaten, it had a thick texture and a texture and flavor of kumage yuba. When seasoning liquid was added to this, it was able to eat more deliciously.
(参考例d1) 冷凍豆腐(対照)
 丸大豆5kgに対して水(10℃)15kgを加え、14時間浸漬した。これを、10メッシュのフルイで浸漬ホエーと浸漬大豆とに分離した。次に、この浸漬大豆を挽き水(20℃)25kgと共にグラインダー((株)長沢機械製作所製。以下、同様)を用いて呉にした。これを、分離機((株)トーファー製。以下、同様)を用いて豆乳(固形分濃度9%)とオカラに分けた。この豆乳を間接加熱装置((株)星高製。以下、同様)を用いて98℃で5分間加熱処理を行った。得られた豆乳を濃縮機((株)日阪製作所製。以下、同様)で真空圧100torrで固形分濃度12%に調整し、濃縮豆乳を得た。
 次に、この濃縮豆乳を75℃に調温し、濃縮豆乳100部に対して、ワキシーコーンスターチ「デリカSE」(日澱化学(株)製)を3部、デキストリン「サンデック250」(三和澱粉工業(株)製)を2部、塩化マグネシウム製剤「マグネスファインTG」(花王(株)製)0.9部を加え、型箱に入れ凝固させた。凝固温度は70℃であった。凝固後、90℃で40分間蒸し加熱を行った。これを、20℃以下に冷却後、所定のサイズに切断し、約-35℃の雰囲気下で急速冷凍し、冷凍豆腐(対照)を得た。
(Reference Example d1) Frozen tofu (control)
15 kg of water (10 ° C.) was added to 5 kg of whole soybeans and immersed for 14 hours. This was separated into soaked whey and soaked soybeans with a 10 mesh sieve. Next, the soaked soybeans were made into kure using a grinder (manufactured by Nagasawa Machinery Co., Ltd., hereinafter the same) together with 25 kg of ground water (20 ° C.). This was separated into soy milk (solid content concentration 9%) and okara using a separator (manufactured by Topher Co., Ltd., hereinafter the same). This soymilk was heat-treated at 98 ° C. for 5 minutes using an indirect heating apparatus (manufactured by Hoshitaka Co., Ltd., hereinafter the same). The obtained soymilk was adjusted to a solid content concentration of 12% at a vacuum pressure of 100 torr with a concentrator (manufactured by Nisaka Manufacturing Co., Ltd., hereinafter the same) to obtain concentrated soymilk.
Next, this concentrated soymilk was adjusted to 75 ° C., and 100 parts of the concentrated soymilk, 3 parts of waxy corn starch “Delica SE” (manufactured by Nissho Chemical Co., Ltd.) and dextrin “Sandeck 250” (Sanwa Starch) 2 parts of Kogyo Kogyo Co., Ltd. and 0.9 parts of magnesium chloride preparation “Magnes Fine TG” (Kao Co., Ltd.) were added and solidified in a mold box. The solidification temperature was 70 ° C. After solidification, steaming and heating were performed at 90 ° C. for 40 minutes. This was cooled to 20 ° C. or lower, cut into a predetermined size, and rapidly frozen in an atmosphere of about −35 ° C. to obtain frozen tofu (control).
(実施例d5,d6) 冷凍豆腐
 参考例d1で得た濃縮豆乳に対して、製造例2で得られた大豆乳化組成物B(固形分濃度18.2%)を、濃縮豆乳:大豆乳化組成物B=70:30(実施例d5)、40:60(実施例d6)の割合で混合し、参考例d1と同様の方法で調製し、冷凍豆腐を得た。
(Examples d5 and d6) Frozen tofu The concentrated soy milk obtained in Reference Example d1 was obtained by adding the soybean emulsified composition B (solid content concentration 18.2%) obtained in Production Example 2 to the concentrated soy milk: soy emulsified composition. Product B = 70: 30 (Example d5), 40:60 (Example d6) were mixed at a ratio and prepared in the same manner as in Reference Example d1 to obtain frozen tofu.
(参考例d2) 冷凍厚揚げ(対照)
 豆乳の固形分濃度16%とした以外は、参考例d1と同様に処理し、冷凍前の豆腐を調製した。この豆腐を180℃、2分間の条件で、ナタネ油でフライし、その後、約-35℃の雰囲気下で急速凍結し、冷凍厚揚げ(対照)を得た。
(Reference Example d2) Frozen deep fried (control)
The tofu before freezing was prepared in the same manner as in Reference Example d1 except that the soymilk solid content concentration was 16%. This tofu was fried in rapeseed oil at 180 ° C. for 2 minutes, and then snap-frozen in an atmosphere of about −35 ° C. to obtain a frozen deep-fried (control).
(実施例d7~d9) 冷凍厚揚げ
 参考例d2で得た濃縮豆乳に対して、製造例2で得られた大豆乳化組成物B(固形分濃度18.2%)を、濃縮豆乳:大豆乳化組成物B=85:15(実施例d7)、70:30(実施例d8)、40:60(実施例d9)の割合で混合し、参考例d1と同様の方法で調製し、冷凍前の豆腐を調製した。この豆腐を180℃、2分間の条件で、ナタネ油でフライし、その後、約-35℃の雰囲気下で急速凍結し、冷凍厚揚げを得た。
(Examples d7 to d9) Frozen deep-frying The concentrated soy milk obtained in Reference Example d2 is obtained by adding the soy emulsion composition B (solid content concentration 18.2%) obtained in Production Example 2 to the concentrated soy milk: soy emulsion. Composition B = 85: 15 (Example d7), 70:30 (Example d8), and mixed at a ratio of 40:60 (Example d9), prepared in the same manner as Reference Example d1, and before freezing Tofu was prepared. This tofu was fried in rapeseed oil at 180 ° C. for 2 minutes, and then snap-frozen in an atmosphere of about −35 ° C. to obtain a deep-fried deep-fried chicken.
(品質評価方法)
 食感は、冷凍豆腐および厚揚げを室温解凍してから官能評価した。滑らかで豆腐らしい食感のものを5点、硬く口溶けの悪いものや澱粉添加によるネタツキのあるものを1点とした。風味は、好ましい豆の風味を感じるものを5点、豆の味を感じないものを1点とした。評価は、5人のパネラーにて5点満点の評点法で評価した。
(Quality evaluation method)
The texture was sensory evaluated after thawing frozen tofu and deep-fried to room temperature. A smooth and tofu-like texture was scored 5 points, a hard and poorly melted mouthfeel, and a grainy texture due to the addition of starch was scored 1 point. As for the flavor, 5 points were given to feel the preferred bean flavor, and 1 point was given to those that did not feel the bean taste. Evaluation was performed by a five-point scoring method with five panelists.
(表9) 冷凍豆腐に対する大豆乳化組成物Bの添加効果
Figure JPOXMLDOC01-appb-I000010
(Table 9) Effect of adding soybean emulsified composition B to frozen tofu
Figure JPOXMLDOC01-appb-I000010
(冷凍豆腐評価)
 参考例d1は、大豆の濃厚な風味が少なくあっさりとした風味であった。また食感は硬めであり、従来の豆腐と同様の食感であった。
 実施例d5の濃縮豆乳:大豆乳化組成物B=70:30の場合は、参考例d1に比べて、大豆の濃厚な(コクのある)風味が感じられ、それでいて青臭くなく、また、食感は軟らかくかつ滑らかで口溶けが良くなり、咀嚼時に豆腐の粒が溶けるような食感であった。
 実施例d6の濃縮豆乳:大豆乳化組成物B=40:60の場合は、実施例d5に比べて、さらに濃厚な大豆風味が感じられ、さらに、食感においては、プリンのように非常に軟らかくかつ滑らかで口溶けが非常によく、クリーミーな食感であった。
(Frozen tofu evaluation)
Reference Example d1 had a light flavor with little rich soybean flavor. Moreover, the texture was hard and was the same texture as the conventional tofu.
In the case of the concentrated soymilk: soy emulsified composition B = 70: 30 in Example d5, the rich (bright) flavor of soybean is felt compared to Reference Example d1, yet it does not smell blue, and the texture is The texture was soft and smooth with good mouth melting, and the tofu grains melted when chewed.
In the case of the concentrated soymilk: soy emulsified composition B = 40: 60 in Example d6, a thicker soybean flavor is felt compared to Example d5, and the texture is very soft like pudding. It was smooth and melted well in the mouth, with a creamy texture.
(表10) 冷凍絹厚揚げに対する大豆乳化組成物Bの添加効果
Figure JPOXMLDOC01-appb-I000011
(Table 10) Additive effect of soybean emulsified composition B on deep-fried frozen silk
Figure JPOXMLDOC01-appb-I000011
(絹厚揚げ評価)
 参考例d2は、大豆の濃厚な風味が少なくあっさりとしていた。また食感は硬く、口の中で直ぐには溶けずに口溶けは悪い食感であった。
 実施例d7の濃縮豆乳:大豆乳化組成物B=85:15の場合は、参考例d2に比べて、やや大豆の風味が感じられ、軟らかく滑らかな食感への変化が感じられた。
 実施例d8の濃縮豆乳:大豆乳化組成物B=70:30の場合は、実施例d7に比べて、より濃厚な大豆の風味が感じられ、さらに、食感においては、軟らかく滑らかな食感であった。
 実施例d9の濃縮豆乳:大豆乳化組成物B=40:60の場合は、実施例d8と比べて、濃厚な大豆の風味がさらに強く感じられ好ましく、食感においても非常に軟らかくかつ滑らかで口溶けも良好であり、非常にクリーミーな食感であった。
(Evaluation of deep-fried silk)
In Reference Example d2, the rich flavor of soybean was small and light. In addition, the texture was hard, and it did not melt immediately in the mouth, but the mouth melted was bad.
In the case of concentrated soymilk: soy emulsified composition B = 85: 15 in Example d7, a slightly soy flavor was felt compared to Reference Example d2, and a change to a soft and smooth texture was felt.
In the case of the concentrated soymilk: soy emulsified composition B = 70: 30 in Example d8, a thicker soybean flavor is felt than in Example d7, and the texture is soft and smooth. there were.
In the case of the concentrated soymilk: soy emulsified composition B = 40: 60 in Example d9, the rich soybean flavor is more strongly felt compared to Example d8, and the texture is very soft and smooth and melts in the mouth. Was also very good and had a very creamy texture.
 以上実施例d5~d9および参考例d1,d2の結果から、十分な添加効果が得られる、豆乳に対する大豆乳化組成物Bの置換率は15%以上が好ましく、より好ましくは20%以上であると濃厚な大豆の風味が付与され軟らかく滑らかな食感とすることができた。 From the results of Examples d5 to d9 and Reference Examples d1 and d2, the substitution rate of the soy emulsion composition B with respect to soy milk is preferably 15% or more, and more preferably 20% or more. A rich soybean flavor was imparted, and a soft and smooth texture was achieved.
(参考例d3) がんもどき1(対照)
  大豆蛋白原料として粉末状分離大豆蛋白「フジプロE」(不二製油(株)製、以下の例において同じ。)を450部、なたね油360部、水1420部をサイレントカッターに投入して、約20℃で混練し、3分間均一に乳化させ、乳化物を調製した。この乳化物に調味料15部、おから18部、小麦粉30部を加え、さらにダイス状のにんじん150部、ごま9部を加え混合し生地を得た。
  生地を成型機で直径58mm、厚み10mm、1個27gに成形した。これを低温部(92~135℃)8分、高温部(155~180℃)1分30秒のフライ加熱を行い、空冷後、-35℃で急速凍結を行い、冷凍のがんもどきを得た。
(Reference Example d3) Cancer pound 1 (control)
As a soy protein raw material, powdered separated soy protein “Fuji Pro E” (Fuji Oil Co., Ltd., the same in the following examples) 450 parts, rapeseed oil 360 parts and water 1420 parts are put into a silent cutter, and about 20 parts. The mixture was kneaded at 0 ° C. and uniformly emulsified for 3 minutes to prepare an emulsion. To this emulsion, 15 parts of seasoning, 18 parts of okara and 30 parts of flour were added, and 150 parts of diced carrot and 9 parts of sesame were added and mixed to obtain a dough.
The dough was formed into a diameter of 58 mm, a thickness of 10 mm, and one piece of 27 g with a molding machine. This is heated in the low temperature part (92 to 135 ° C) for 8 minutes and in the high temperature part (155 to 180 ° C) for 1 minute and 30 seconds. After air cooling, it is rapidly frozen at -35 ° C to obtain frozen cancer. It was.
(実施例d10) がんもどき2(大豆乳化組成物Bを配合)
 参考例d3のがんもどきの製造において、水を製造例2で得られた大豆乳化組成物Bで全量置換し、その他は同様にしてがんもどきを得た。
 得られたがんもどきは、内部はスポンジ状組織を有し、これまでのがんもどきよりも大きく、煮込んだ後もボリュームを維持しながら、調味液をよく含み、従来にないジューシーな口あたりの食感を有していた。さらに風味は大豆本来のコク味も有していた。
(Example d10) Cancer pounding 2 (with soybean emulsified composition B)
In the preparation of the cancer mushroom of Reference Example d3, water was completely replaced with the soybean emulsified composition B obtained in Production Example 2, and the others were similarly obtained.
The resulting cancer mushroom has a sponge-like structure inside, is larger than conventional cancer mushrooms, maintains a volume even after boiling, contains a seasoning liquid, and has an unprecedented juicy mouthfeel. Had a texture. In addition, the flavor had the original richness of soybeans.
(比較例d2)
 参考例d3のがんもどきの製造法において、水を丸大豆豆乳(固形分9.1%、たん白質4.7%、油分3.6%)で全量置換し、その他は同様にしてがんもどきを得た。
(Comparative Example d2)
In the method for producing cancer cancer of Reference Example d3, the whole amount of water was replaced with whole soybean soy milk (solid content: 9.1%, protein: 4.7%, oil content: 3.6%). I got back.
(比較例d3)
 参考例d3のがんもどきの製造法において、水の代わりに練り込み用クリーム「ミルレア」(固形分28.4%、油分15.0%、不二製油(株)製)で全量置換し、その他は同様にしてがんもどきを得た。
(Comparative Example d3)
In the cancer-drinking production method of Reference Example d3, the entire amount was replaced with a kneading cream “Milrea” (solid content 28.4%, oil content 15.0%, manufactured by Fuji Oil Co., Ltd.) instead of water. I got cancer.
(実施例d11~d14) がんもどき3(大豆乳化組成物Bの配合量変化)
 参考例d3のがんもどきの製造法において、製造例2で得られた大豆乳化組成物Bを生地仕込みあたり、142部、355部、710部、1420部を配合し、生地中に含まれる蛋白質量が等しくなるよう、加水調整を行い、その他は同様にして最終的に水との置換率が10%、25%、50%、100%の各がんもどきを得た(実施例d11、d12、d13、d14)。得られたがんもどきは、大豆乳化組成物Bの配合量が多いほど、フライ後のボリュームがあり、調味液の含みも良いがんもどきが得られた。
(Examples d11 to d14) Cancer return 3 (change in blending amount of soybean emulsified composition B)
Proteins contained in dough by blending 142 parts, 355 parts, 710 parts and 1420 parts of soybean emulsified composition B obtained in Production Example 2 in the dough preparation method of Reference Example d3 per dough preparation The amount of water was adjusted so that the amounts were equal, and in the same manner, the other cancers were finally obtained in the same manner with a water substitution rate of 10%, 25%, 50%, and 100% (Examples d11 and d12). , D13, d14). As for the obtained cancer mushroom, as the blended amount of the soybean emulsified composition B was increased, the volume after fried food was increased, and a cancer mushroom that contained a seasoning liquid was obtained.
(評価方法)
 得られたがんもどきは、生地の膨化度合いを目視で観察した上で、長辺(X)、短辺(Y)、厚み(H)のサイズをノギスで測定し、5個の平均値(mm)から体積を算出し、フライ後の膨化状態を確認した。なお、体積(V)は、長辺と短辺の平均値の1/2を半径(Rcm)とし、V≒R×3.14×H として算出した。数値は参考例3の体積を100としたときの比率(%)で表した。
 そして、和風の調味液(しょうゆ大さじ2、砂糖大さじ4、みりん小さじ4、酒小さじ4、だし汁800cc)にて、約15分程度煮込んだ後、取り出して重量を測定した。そしてがんもどきの調味液の吸収倍率を煮込み前がんもどき重量に対する煮込み後がんもどき重量の比率(%)で測定した。
 さらにがんもどきの風味、形状(膨らみ)及び吸液性について、(+++)非常に良好、(++)良好、(+)やや良好、(±)普通、(-)悪いの5段階で評価した。
(Evaluation methods)
The obtained cancer squirrel was measured by visually observing the degree of swelling of the dough and measuring the long side (X), short side (Y), and thickness (H) with a caliper. The volume was calculated from mm), and the expanded state after the frying was confirmed. The volume (V) was calculated as V≈R 2 × 3.14 × H, where ½ of the average value of the long side and short side was the radius (Rcm). The numerical value was expressed as a ratio (%) when the volume of Reference Example 3 was 100.
Then, it was boiled for about 15 minutes in a Japanese-style seasoning liquid (2 tablespoons of soy sauce, 4 tablespoons of sugar, 4 teaspoons of mirin, 4 teaspoons of sake, 800 cc of soup stock), then taken out and weighed. Then, the absorption ratio of the seasoning liquid of the cancer pounding was measured by the ratio (%) of the cancer pounding weight after boiling to the weight of the cancer pounding before cooking.
Furthermore, the flavor, shape (swelling) and liquid absorbency of cancer-likeness were evaluated in five levels: (++++) very good, (++) good, (+) slightly good, (±) normal, (−) bad. .
(表11)
Figure JPOXMLDOC01-appb-I000012
(Table 11)
Figure JPOXMLDOC01-appb-I000012
(評価結果)
 実施例d10~d14、比較例d2、d3から、十分な添加効果が得られる水に対する大豆乳化組成物Bの置換率の目安としては、水に対する大豆乳化組成物Bの置換率が 10%以上が好ましく、より好ましくは25%以上であり、更に好ましくは50%以上であると、フライ後の形状に厚みがあり、ボリュームのあるがんもどきを得ることができた。内部はキメの細かなスポンジ状組織を有し、煮込んだ後は調味液を良く含み、ジューシーな味わいのあるがんもどきを得ることができた。
(Evaluation results)
From Examples d10 to d14 and Comparative Examples d2 and d3, as a measure of the substitution rate of the soy emulsified composition B with respect to water that provides a sufficient addition effect, the substitution rate of the soy emulsified composition B with respect to water is 10% or more. If it is preferably 25% or more, more preferably 50% or more, the shape after frying has a thickness and a cancerous rejuvenation can be obtained. The inside had a fine sponge-like structure, and after boiling it well contained the seasoning liquid, and it was possible to obtain a succulent and thrilling cancer.
(参考例d4) 油揚げ1(対照)
 粉末状分離大豆たん白500部、油脂(パーム油)60部、水1550部、コーンスターチ25部、食塩5部をサイレントカッターに投入し、約20℃で混練し、3分間均一に混合乳化し、乳化物を調製した。その後、塩化マグネシウム12部を水15部に溶かしたものを加え、更に1分間混合させ、適度な固さの生地が調製できた。この生地を成型機を用いて、66.5×46mm、15.5gに成型した。これを100×77mmの型枠を使用した3段フライヤー(70℃で4分、110℃で2分、170℃で4分)でフライし、油揚げを得た。
(Reference example d4) Fried chicken 1 (control)
500 parts of powdered soy protein, 60 parts of fat (palm oil), 1550 parts of water, 25 parts of corn starch and 5 parts of salt are put into a silent cutter, kneaded at about 20 ° C., and uniformly mixed and emulsified for 3 minutes. An emulsion was prepared. Thereafter, 12 parts of magnesium chloride dissolved in 15 parts of water was added, and the mixture was further mixed for 1 minute to prepare a dough having an appropriate hardness. This dough was molded into 65.5 × 46 mm, 15.5 g using a molding machine. This was fried in a three-stage fryer (4 minutes at 70 ° C., 2 minutes at 110 ° C., 4 minutes at 170 ° C.) using a 100 × 77 mm mold to obtain fried chicken.
(実施例d15) 油揚げ2(大豆乳化組成物Bを配合)
 参考例d4の油揚げの製造において、水1550部のうち760部を製造例2で得られた大豆乳化組成物Bで置換した以外は、参考例d4と同様にして、油揚げを得た。
 得られた油揚げは、ふっくらとした外観を有し、内部はキメの細かい組織であった。従来の油揚げに比べ、大豆由来のコク味が付与された風味となっており、調味液で着味した場合、調味液の吸収が良く、ジューシーな味付け油揚げが得られた。
 この油揚げをさらに油揚げと調味液の重量が1:1となる程度に脱液した後、加熱乾燥を行うと 調味前に近いボリューム(厚み)のある油揚げが得られた。この味付け乾燥油揚げの湯戻り状態も良く、調味液をよく含んだ油揚げが得られた。
(Example d15) Deep-fried oil 2 (containing soybean emulsified composition B)
In the production of fried chicken of Reference Example d4, fried chicken was obtained in the same manner as Reference Example d4 except that 760 parts of 1550 parts of water were replaced with the soybean emulsified composition B obtained in Production Example 2.
The obtained fried chicken had a plump appearance and the inside was a fine texture. Compared to conventional fried chicken, it has a flavor with a rich soybean-derived taste, and when seasoned with a seasoning liquid, the seasoning liquid is well absorbed and a juicy seasoned fried food is obtained.
This fried chicken was further fried and drained so that the weight of the seasoning liquid was 1: 1, and then heat-dried to obtain a deep-fried fried chicken with a volume (thickness) close to that before seasoning. The seasoned dry fried chicken was well returned to the hot water, and a deep-fried fried chicken well containing the seasoning liquid was obtained.
(比較例d4)
 参考例d4の油揚げの製造において、水1550部のうち760部を丸大豆豆乳(固形分9.1%、たん白質4.7%、油分3.6%)で置換した以外は、参考例d4と同様にして、油揚げを得た。
(Comparative Example d4)
Reference Example d4, except that 760 parts of 1550 parts of water were replaced with whole soybean soy milk (solid content: 9.1%, protein: 4.7%, oil content: 3.6%). In the same manner as above, fried chicken was obtained.
(比較例d5)
 製造例d4の油揚げの製造において、水1550部のうち760部を練り込み用クリーム「ミルレア」(固形分28.4%、油分15.0%、不二製油(株)製)で置換した以外は、参考例d4と同様にして、油揚げを得た。
(Comparative Example d5)
Reference Example, except that 760 parts of water 1550 parts were replaced with kneading cream “Milrea” (solid content 28.4%, oil content 15.0%, manufactured by Fuji Oil Co., Ltd.) Fried chicken was obtained in the same manner as d4.
(実施例d16~d19) 油揚げ3(大豆乳化組成物Bの配合量変化)
 参考例d4の油揚げの製造において、粉末状分離大豆蛋白を500部、なたね油60部とし、製造例2で得られた大豆乳化組成物Bを生地仕込みあたり、150部、380部、760部、1525部を配合し、生地中に含まれる蛋白質量が等しくなるよう加水調整を行い、その他は同様にして最終的に水との置換率が10%、25%、50%、85%の各油揚げを得た(実施例d16、d17、d18、d19)。
(Examples d16 to d19) Deep-fried oil 3 (change in blending amount of soybean emulsified composition B)
In the production of deep-fried oil of Reference Example d4, 500 parts of powdered soy protein and 60 parts of rapeseed oil were used, and the soybean emulsified composition B obtained in Production Example 2 was 150 parts, 380 parts, 760 parts, 1525 per dough preparation. The water content is adjusted so that the amount of protein contained in the dough is equal, and the rest of the frying is 10%, 25%, 50%, and 85%. Obtained (Examples d16, d17, d18, d19).
(評価方法)
 得られた油揚げは、生地の膨化度合いを目視で観察した上で、長辺、短辺、厚みのサイズをノギスで測定し、5個の平均値(mm)から、体積を算出し、フライ後の膨化状態を確認した。数値は参考例4の体積を100としたときの比率(%)で表した。
 そして、得られた油揚げを和風の調味液で、約3分程度煮込んだ後、取り出して重量を測定した。そして油揚げの調味液の吸収倍率を煮込み前重量に対する煮込み後重量の比率(%)で測定した。
 さらに油揚げの風味、形状(膨らみ)及び吸液性について、(+++)非常に良好、(++)良好、(+)やや良好、(±)普通、(-)悪いの5段階で評価した。
(Evaluation methods)
The resulting deep-fried fried food was measured by visually observing the degree of expansion of the dough, then measuring the size of the long side, short side, and thickness with calipers, calculating the volume from the average value of 5 pieces (mm), The swelling state of was confirmed. The numerical value was expressed as a ratio (%) when the volume of Reference Example 4 was 100.
And the obtained fried chicken was boiled for about 3 minutes with a Japanese-style seasoning liquid, then taken out and weighed. And the absorptivity of the fried seasoning liquid was measured by the ratio (%) of the weight after cooking to the weight before cooking.
Furthermore, the flavor, shape (bulge) and liquid absorbency of fried chicken were evaluated in five levels: (++++) very good, (++) good, (+) slightly good, (±) normal, and (−) bad.
(表12)
Figure JPOXMLDOC01-appb-I000013
(Table 12)
Figure JPOXMLDOC01-appb-I000013
(評価結果)
 実施例d15~d19、比較例d4、d5から、十分な添加効果が得られる水に対する大豆乳化組成物Bの置換率の目安としては、水に対する大豆乳化組成物Bの置換率が 10%以上が好ましく、より好ましくは25%以上であり、更に好ましくは50%以上であると、フライ後の形状に厚みがあり、ボリュームのある油揚げを得ることができた。内部はキメの細かなスポンジ状組織を有し、煮込んだ後は調味液を良く含み、ジューシーな味わいのある油揚げを得ることができた。
(Evaluation results)
From Examples d15 to d19 and Comparative Examples d4 and d5, as a measure of the substitution rate of the soy emulsified composition B with respect to water that provides a sufficient addition effect, the substitution rate of the soy emulsified composition B with respect to water is 10% or more. If it is preferably 25% or more, more preferably 50% or more, the shape after frying has a thickness, and a deep fried chicken can be obtained. The inside had a fine sponge-like structure, and after boiling it well contained the seasoning liquid, and a fried chicken with a juicy taste could be obtained.
<第五発明の具体例>
(実施例e1、比較例e1) 血清コレステロール低減試験
 表13に示す配合の餌をラットに給餌し、血清コレステロールを測定した。
<Specific examples of the fifth invention>
(Example e1, comparative example e1) Serum cholesterol reduction test Serum cholesterol was measured by feeding a rat with the formulation shown in Table 13 to rats.
(表13) 餌配合 
Figure JPOXMLDOC01-appb-I000014
(Table 13) Feed formulation
Figure JPOXMLDOC01-appb-I000014
 注)餌中の粗蛋白質含量(CP)=Casein10%+サンプル由来10%とし、餌中油分は「大豆乳化組成物B」食含有量(20%)に合わせて足りない分を大豆油で補って調整し、蛋白質量、油分量をそろえた2種の餌を作製した。CaseinのCPは88%であった。 Note) Crude protein content in the diet (CP) = Casein 10% + sample-derived 10%, and the oil content in the diet is supplemented with soybean oil to meet the “Soy emulsified composition B” food content (20%). Two types of baits with the same protein mass and oil content were prepared. Casein's CP was 88%.
○試験方法
 動物    :Zucker fattyラット
 動物数、期間:n=6×2群、2週間
 評価サンプル:Casein、大豆乳化組成物B
 操作    :<体重、摂食量> 毎日測定
        <採血>     計2回(途中経過、最終解剖時)
 分析    :<コレステロール関連> 血清コレステロール濃度(富士ドライケム)
○ Test method Animals: Zucker fatty rats Number of animals, period: n = 6 × 2 groups, 2 weeks Evaluation sample: Casein, soy emulsion composition B
Operation: <Body weight, food intake> Daily measurement <Blood collection> Total 2 times (on the way, during final dissection)
Analysis: <Cholesterol-related> Serum cholesterol concentration (Fuji Dry Chem)
○試験結果
 血清コレステロール濃度は、2週間の摂食により大豆乳化組成物Bを用いた試験区(実施例e1)では、Caseinを用いた試験区(比較例e1)と比較して有意に低い値を示した。試験開始時点での同ラットにおける血清コレステロール濃度は144.7±4.7mg/dl(平均値±SE)であった。なお、Zucker fattyラットは顕著な肥満を示す突然変異体であり、加齢に伴い血清コレステロール濃度は上昇することが知られている。
 比較例e1では、Zucker fattyラットにおける加齢に伴う血清コレステロール上昇を、カゼインを用いた餌は何ら抑制しないことを示す一方、実施例e1では、Zucker fattyラットの加齢に伴う血清コレステロール上昇を積極的に抑制することが示された。
○ Test result Serum cholesterol concentration is significantly lower in the test group using soybean emulsified composition B (Example e1) after 2 weeks of feeding than in the test group using Casein (Comparative Example e1) showed that. The serum cholesterol concentration in the rat at the start of the test was 144.7 ± 4.7 mg / dl (mean value ± SE). Zucker fatty rats are mutants exhibiting marked obesity, and it is known that serum cholesterol levels increase with age.
Comparative Example e1 shows that serum cholesterol elevation associated with aging in Zucker fatty rats does not inhibit any casein-based diet, whereas Example e1 actively promotes serum cholesterol elevation associated with aging in Zucker fatty rats. Has been shown to be suppressed.
(実施例f1、比較例f1) 尿中NAG活性低減試験
 表14に示す配合の餌をラットに給餌し、尿中のNAG(β-N-アセチル-D-グルコサミニダーゼ)を測定した。NAGは臓器に広く分布している一方、かなり大きな酵素であるため血清中の酵素が尿に出ることはほとんどなく、尿におけるNAGの量を定量することで、尿細管上皮細胞の変性や破壊のような傷害を知ることができる。
(表14) 餌配合 
Figure JPOXMLDOC01-appb-I000015
(Example f1, comparative example f1) Urinary NAG activity reduction test Rats were fed with the diet shown in Table 14, and NAG (β-N-acetyl-D-glucosaminidase) in urine was measured. NAG is widely distributed in organs, but since it is a fairly large enzyme, serum enzymes rarely appear in urine. By quantifying the amount of NAG in urine, degeneration and destruction of tubular epithelial cells You can know such injuries.
(Table 14) Feed formulation
Figure JPOXMLDOC01-appb-I000015
○試験方法
 動物    :Zucker fattyラット
 動物数、期間:n=6×2群、2週間
 評価サンプル:Casein、大豆乳化組成物B
 操作    :<体重、摂食量> 毎日測定
        <採血>     計2回(途中経過、最終解剖時)
 分析    :尿中NAG活性(NAGテストシオノギ)、尿中蛋白量(トネインTP)
○ Test method Animals: Zucker fatty rats Number of animals, period: n = 6 × 2 groups, 2 weeks Evaluation sample: Casein, soy emulsion composition B
Operation: <Body weight, food intake> Daily measurement <Blood collection> Total 2 times (on the way, during final dissection)
Analysis: Urinary NAG activity (NAG Test Shionogi), Urinary protein (Tonein TP)
○試験結果
 尿中NAG活性は、2週間の摂食により大豆乳化組成物Bを用いた試験区では、Caseinを用いた試験区と比較して有意に低い値を示した。なお、Zucker fattyラットは顕著な肥満を示す突然変異体であり、加齢に伴い合併症として腎症が発生することが知られている。
 比較例f1では、Zucker fattyラットにおける加齢に伴う腎症の発生を、カゼインを用いた餌は何ら抑制しないことを示す一方、実施例f1では、積極的に抑制することが示された。
○ Test result The urinary NAG activity showed a significantly lower value in the test group using soybean emulsified composition B by feeding for 2 weeks compared to the test group using Casein. The Zucker fatty rat is a mutant exhibiting remarkable obesity, and nephropathy is known to occur as a complication with aging.
In Comparative Example f1, it was shown that the occurrence of nephropathy accompanying aging in Zucker fatty rats was not suppressed at all by Example f1, while it was shown that it was positively suppressed.
 本第一~第五発明は、大豆由来原料が各種用途で原料に使用されている様々な食品の製造に利用することが可能である。
 例えば、洋菓子類、パン類、デザート類、飲料類、スープ類、ソース類などの製造において、牛乳、生クリーム、全脂粉乳などの乳製品の一部または全部を、本第一発明の風味が大幅に改善された大豆乳化組成物に置換することにより、風味が大幅に改善された大豆由来原料含有飲食品を提供することができる。
 また、例えば本第五発明の血清コレステロール低減用組成物又は腎機能改善用組成物を用いることで、需要者は食生活を通じ血清コレステロールを低減させ、又は腎機能を改善させることができる。よって食品業界においては、新たな需要を喚起する製品を開発することが可能となる。
The first to fifth inventions can be used for the production of various foods in which soybean-derived materials are used as raw materials for various purposes.
For example, in the manufacture of confectionery, breads, desserts, beverages, soups, sauces, etc., a part or all of dairy products such as milk, fresh cream, whole milk powder, etc. By substituting with a greatly improved soybean emulsified composition, a food-derived food containing a soybean-derived material with a significantly improved flavor can be provided.
In addition, for example, by using the composition for reducing serum cholesterol or the composition for improving renal function of the fifth invention, a consumer can reduce serum cholesterol or improve renal function through eating habits. Therefore, in the food industry, it is possible to develop products that stimulate new demand.

Claims (15)

  1. 乾物あたりの蛋白質含量が25重量%以上であり脂質含量(クロロホルム/メタノール混合溶媒抽出物としての含量をいう。以下の請求項において同じ。)が蛋白質含量に対して100重量%以上でありかつLCI値が55%以上である大豆乳化組成物、又は、該大豆乳化組成物を含む原料を乳酸発酵もしくは酸添加により酸性化して得られる酸性大豆素材を含有することを特徴とする、大豆由来原料含有飲食品。 The protein content per dry matter is 25% by weight or more, the lipid content (the content as a chloroform / methanol mixed solvent extract, the same in the following claims) is 100% by weight or more with respect to the protein content and LCI A soybean-derived material containing a soybean emulsion composition having a value of 55% or more, or an acidic soybean material obtained by acidifying a raw material containing the soybean emulsion composition by lactic acid fermentation or acid addition Food and drink.
  2. 乾物あたりの食物繊維含量が10重量%以下であって乾物あたりの脂質含量が35重量%以上である請求項1記載の大豆乳化組成物、又は該大豆乳化組成物を含む原料を乳酸発酵もしくは酸添加により酸性化して得られる酸性大豆素材を含有することを特徴とする、大豆由来原料含有飲食品。 2. The soybean emulsified composition according to claim 1, wherein the dietary fiber content per dry matter is 10% by weight or less and the lipid content per dry matter is 35% by weight or more. A soy-derived material-containing food or drink comprising an acidic soybean material obtained by acidification by addition.
  3. 請求項1記載の大豆乳化組成物又は酸性大豆素材を含むことを特徴とする、乳製品もしくは発酵乳製品代替物。 A dairy product or fermented dairy product substitute comprising the soybean emulsified composition according to claim 1 or an acidic soybean material.
  4. 請求項3記載の乳製品もしくは発酵乳製品代替物が、乳製品もしくは発酵乳製品の一部又は全部の代替として使用されていることを特徴とする、大豆由来原料含有飲食品。 The dairy product or fermented dairy product substitute according to claim 3 is used as a substitute for part or all of the dairy product or fermented dairy product.
  5. 請求項1記載の大豆乳化組成物を含む原料を、乳酸発酵又は酸添加により酸性化して得られることを特徴とする酸性大豆素材。 An acidic soybean material obtained by acidifying a raw material containing the soybean emulsified composition according to claim 1 by lactic acid fermentation or acid addition.
  6. 請求項1記載の大豆乳化組成物を含有することを特徴とする、卵黄代替組成物。 An egg yolk substitute composition comprising the soybean emulsified composition according to claim 1.
  7. 請求項2記載の大豆乳化組成物を含有することを特徴とする、卵黄代替組成物。 An egg yolk substitute composition comprising the soybean emulsified composition according to claim 2.
  8. 請求項6記載の卵黄代替組成物が、卵黄の一部又は全部の代替として使用されていることを特徴とする卵黄代替食品。 The egg yolk substitute composition according to claim 6, wherein the egg yolk substitute composition is used as a substitute for part or all of egg yolk.
  9. 請求項1記載の大豆乳化組成物が、大豆原料の一部又は全部として使用されていることを特徴とする大豆加工食品。 The soybean processed food according to claim 1, wherein the soybean emulsified composition according to claim 1 is used as part or all of a soybean raw material.
  10. 請求項2記載の大豆乳化組成物が、大豆原料の一部又は全部として使用されていることを特徴とする大豆加工食品。 A soybean processed food, wherein the soybean emulsified composition according to claim 2 is used as part or all of a soybean raw material.
  11. 請求項1記載の大豆乳化組成物を含有する、血清コレステロール低減用もしくは腎機能改善用組成物。 A composition for reducing serum cholesterol or improving renal function, comprising the soybean emulsified composition according to claim 1.
  12. 請求項2記載の大豆乳化組成物を含有する、血清コレステロール低減用もしくは腎機能改善用組成物。 A composition for reducing serum cholesterol or improving renal function, comprising the soybean emulsified composition according to claim 2.
  13. 請求項11記載の血清コレステロール低減用もしくは腎機能改善用組成物を用いた食品。 A food using the composition for reducing serum cholesterol or improving renal function according to claim 11.
  14. 飲食品の製造において、乾物あたりの蛋白質含量が25重量%以上であり脂質含量が蛋白質含量に対して100重量%以上でありかつLCI値が55%以上である大豆乳化組成物を、乳製品、卵黄及び大豆原料から選択される原料の一部又は全部と代替して使用し、あるいは、血清コレステロール低減用もしくは腎機能改善用組成物として使用することを特徴とする、大豆由来原料含有飲食品の製造法。 In the production of food and drink, a soybean emulsion composition having a protein content per dry matter of 25% by weight or more, a lipid content of 100% by weight or more with respect to the protein content, and an LCI value of 55% or more, A soy-derived material-containing food or drink characterized by being used as a substitute for part or all of a raw material selected from egg yolk and soy raw material, or as a composition for reducing serum cholesterol or improving renal function Manufacturing method.
  15. 乾物あたりの蛋白質含量が25重量%以上であり脂質含量が蛋白質含量に対して100重量%以上でありかつLCI値が55%以上である大豆乳化組成物を、乳製品、卵黄及び大豆原料から選択される原料の一部もしくは全部と代替して使用し、あるいは、血清コレステロール低減用もしくは腎機能改善用組成物として使用することにより、大豆由来原料含有飲食品の風味又は食感を改良する方法。 A soy emulsified composition having a protein content per dry matter of 25% by weight or more, a lipid content of 100% by weight or more with respect to the protein content, and an LCI value of 55% or more is selected from dairy products, egg yolk and soy materials. A method for improving the flavor or texture of a soy-derived raw material-containing food or drink by using it in place of part or all of the raw material to be used, or as a composition for reducing serum cholesterol or improving renal function.
PCT/JP2012/063110 2011-06-07 2012-05-23 Novel application of soybean emulsion composition to soybean-derived raw material-containing food or beverage WO2012169347A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US14/123,837 US9101158B2 (en) 2011-06-07 2012-05-23 Application of soybean emulsion composition to soybean-derived raw material-containing food or beverage
KR1020137034124A KR101576797B1 (en) 2011-06-07 2012-05-23 Novel application of soybean emulsion composition to soybean-derived raw material-containing food or beverage
CN201280027929.2A CN103596451B (en) 2011-06-07 2012-05-23 Soybean emulsification composition is containing from the novelty teabag in the diet product of soybean material

Applications Claiming Priority (18)

Application Number Priority Date Filing Date Title
JP2011-126868 2011-06-07
JP2011126868 2011-06-07
JP2011-126871 2011-06-07
JP2011126871 2011-06-07
JP2011-145101 2011-06-30
JP2011145101 2011-06-30
JP2011268484A JP2013118841A (en) 2011-12-08 2011-12-08 Renal function-improving composition
JP2011-268484 2011-12-08
JP2011268483A JP5609854B2 (en) 2011-12-08 2011-12-08 Composition for reducing serum cholesterol
JP2011-268483 2011-12-08
JP2011270814 2011-12-12
JP2011-270860 2011-12-12
JP2011270843A JP5488576B2 (en) 2011-06-30 2011-12-12 Texture or flavor improver for manufacturing processed soybean foods and processed soybean foods
JP2011-271007 2011-12-12
JP2011271007A JP5660018B2 (en) 2011-06-07 2011-12-12 Egg yolk substitute composition and food using the same
JP2011270860A JP5585571B2 (en) 2011-06-07 2011-12-12 Acid soy material and food using the same
JP2011-270814 2011-12-12
JP2011-270843 2011-12-12

Publications (1)

Publication Number Publication Date
WO2012169347A1 true WO2012169347A1 (en) 2012-12-13

Family

ID=47295915

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2012/063110 WO2012169347A1 (en) 2011-06-07 2012-05-23 Novel application of soybean emulsion composition to soybean-derived raw material-containing food or beverage

Country Status (1)

Country Link
WO (1) WO2012169347A1 (en)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103250807A (en) * 2013-04-11 2013-08-21 李月素 Manufacturing method for sweet vegetarian chicken with purple sweet potato starch interlayer
CN103262898A (en) * 2013-04-07 2013-08-28 李玉汉 Production method of hawthorn vegetarian chicken
CN103380823A (en) * 2013-06-20 2013-11-06 马鞍山市黄池食品(集团)有限公司 Liver-protecting bean curd sticks containing coix seed powder and preparation method thereof
JP2014110768A (en) * 2012-12-05 2014-06-19 Fuji Oil Co Ltd Bread and production method thereof
WO2015122424A1 (en) * 2014-02-12 2015-08-20 不二製油株式会社 Egg replacement for spongy food
JP2016185096A (en) * 2015-03-27 2016-10-27 不二製油株式会社 Soybean protein-containing liquid food and drink and method for producing the same
CN107048209A (en) * 2017-04-14 2017-08-18 福建师范大学福清分校 Preparation method of peanut fermented bean curd sauce
JP2018042520A (en) * 2016-09-16 2018-03-22 和光食品株式会社 Condensed soybean milk-containing oil-in-water type emulsion and manufacturing method therefor
WO2018181829A1 (en) * 2017-03-30 2018-10-04 不二製油グループ本社株式会社 Method for producing cheese-like tofu
JP2019134718A (en) * 2013-10-24 2019-08-15 旭松食品株式会社 Method for producing frozen tofu
WO2019188788A1 (en) * 2018-03-29 2019-10-03 不二製油グループ本社株式会社 Method for manufacturing soybean paste food or gel food
EP3590348A4 (en) * 2017-03-02 2020-08-19 Fuji Oil Holdings Inc. Method for producing fresh cheese-like vegetable food product
JP2021016348A (en) * 2019-07-19 2021-02-15 不二製油株式会社 Manufacturing method of sheet-like protein-containing food product
JP6963707B1 (en) * 2021-04-23 2021-11-10 キユーピー株式会社 Liquid egg substitute composition and heat coagulated product
WO2022075252A1 (en) * 2020-10-05 2022-04-14 不二製油グループ本社株式会社 Plant-based soup base and method for producing plant-based soup base

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5985266A (en) * 1982-11-05 1984-05-17 Asahimatsu Shokuhin Kk Preparation of soybean protein curd having creamy texture
JP2000102346A (en) * 1998-09-30 2000-04-11 Fuji Oil Co Ltd Creamy food using soybean food material
JP2002034503A (en) * 2000-07-24 2002-02-05 Tajimaya Shokuhin Kk Mayonnaise-like food produced by using concentrated soya milk and method for producing the food
WO2006129647A1 (en) * 2005-05-30 2006-12-07 Fuji Oil Company, Limited Fractionated soybean protein material, processed soybean suitable for the material, and processes for production of the soybean protein material and the processed soybean
JP2008148633A (en) * 2006-12-18 2008-07-03 Morinaga Milk Ind Co Ltd Lactic acid bacterium-fermented food containing bean curd puree
JP2009136158A (en) * 2007-12-03 2009-06-25 Fuji Oil Co Ltd Method for producing cheese-like soybean fermented food
JP2009528847A (en) * 2006-03-03 2009-08-13 スペシャルティ プロテイン プロデューサーズ インコーポレイテッド Method for separating fat from soy material and composition produced by the method
WO2009110504A1 (en) * 2008-03-04 2009-09-11 不二製油株式会社 Soybean protein material for patients with renal disease and foods made from the same
JP2009280517A (en) * 2008-05-21 2009-12-03 Nisshin Oillio Group Ltd Powder material for lowering blood cholesterol level and foodstuff or fodder containing the powder material for mammal
JP2010519928A (en) * 2007-03-02 2010-06-10 スペシャルティ プロテイン プロデューサーズ リミテッド ライアビリティ カンパニー Method for separating fat from soy material and composition produced therefrom
WO2011155328A1 (en) * 2010-06-07 2011-12-15 不二製油株式会社 Fat-reduced soybean protein material and soybean emulsion composition, and processes for production thereof

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5985266A (en) * 1982-11-05 1984-05-17 Asahimatsu Shokuhin Kk Preparation of soybean protein curd having creamy texture
JP2000102346A (en) * 1998-09-30 2000-04-11 Fuji Oil Co Ltd Creamy food using soybean food material
JP2002034503A (en) * 2000-07-24 2002-02-05 Tajimaya Shokuhin Kk Mayonnaise-like food produced by using concentrated soya milk and method for producing the food
WO2006129647A1 (en) * 2005-05-30 2006-12-07 Fuji Oil Company, Limited Fractionated soybean protein material, processed soybean suitable for the material, and processes for production of the soybean protein material and the processed soybean
JP2009528847A (en) * 2006-03-03 2009-08-13 スペシャルティ プロテイン プロデューサーズ インコーポレイテッド Method for separating fat from soy material and composition produced by the method
JP2008148633A (en) * 2006-12-18 2008-07-03 Morinaga Milk Ind Co Ltd Lactic acid bacterium-fermented food containing bean curd puree
JP2010519928A (en) * 2007-03-02 2010-06-10 スペシャルティ プロテイン プロデューサーズ リミテッド ライアビリティ カンパニー Method for separating fat from soy material and composition produced therefrom
JP2009136158A (en) * 2007-12-03 2009-06-25 Fuji Oil Co Ltd Method for producing cheese-like soybean fermented food
WO2009110504A1 (en) * 2008-03-04 2009-09-11 不二製油株式会社 Soybean protein material for patients with renal disease and foods made from the same
JP2009280517A (en) * 2008-05-21 2009-12-03 Nisshin Oillio Group Ltd Powder material for lowering blood cholesterol level and foodstuff or fodder containing the powder material for mammal
WO2011155328A1 (en) * 2010-06-07 2011-12-15 不二製油株式会社 Fat-reduced soybean protein material and soybean emulsion composition, and processes for production thereof

Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014110768A (en) * 2012-12-05 2014-06-19 Fuji Oil Co Ltd Bread and production method thereof
CN103262898A (en) * 2013-04-07 2013-08-28 李玉汉 Production method of hawthorn vegetarian chicken
CN103250807A (en) * 2013-04-11 2013-08-21 李月素 Manufacturing method for sweet vegetarian chicken with purple sweet potato starch interlayer
CN103380823A (en) * 2013-06-20 2013-11-06 马鞍山市黄池食品(集团)有限公司 Liver-protecting bean curd sticks containing coix seed powder and preparation method thereof
JP2019134718A (en) * 2013-10-24 2019-08-15 旭松食品株式会社 Method for producing frozen tofu
WO2015122424A1 (en) * 2014-02-12 2015-08-20 不二製油株式会社 Egg replacement for spongy food
JPWO2015122424A1 (en) * 2014-02-12 2017-03-30 不二製油株式会社 Sponge food egg substitute
JP2016185096A (en) * 2015-03-27 2016-10-27 不二製油株式会社 Soybean protein-containing liquid food and drink and method for producing the same
JP2018042520A (en) * 2016-09-16 2018-03-22 和光食品株式会社 Condensed soybean milk-containing oil-in-water type emulsion and manufacturing method therefor
US11202455B2 (en) 2017-03-02 2021-12-21 Fuji Oil Holdings Inc. Method for producing vegetable fresh cheese-like food product
EP3590348A4 (en) * 2017-03-02 2020-08-19 Fuji Oil Holdings Inc. Method for producing fresh cheese-like vegetable food product
WO2018181829A1 (en) * 2017-03-30 2018-10-04 不二製油グループ本社株式会社 Method for producing cheese-like tofu
JPWO2018181829A1 (en) * 2017-03-30 2020-02-27 不二製油株式会社 Manufacturing method of cheese-like tofu
CN107048209A (en) * 2017-04-14 2017-08-18 福建师范大学福清分校 Preparation method of peanut fermented bean curd sauce
CN107048209B (en) * 2017-04-14 2020-04-17 福建师范大学福清分校 Preparation method of peanut fermented bean curd sauce
JPWO2019188788A1 (en) * 2018-03-29 2021-03-25 不二製油株式会社 Manufacturing method of soybean paste-like food or gel-like food
WO2019188788A1 (en) * 2018-03-29 2019-10-03 不二製油グループ本社株式会社 Method for manufacturing soybean paste food or gel food
JP7279708B2 (en) 2018-03-29 2023-05-23 不二製油株式会社 Method for producing soybean paste-like food or gel-like food
JP2021016348A (en) * 2019-07-19 2021-02-15 不二製油株式会社 Manufacturing method of sheet-like protein-containing food product
JP7354641B2 (en) 2019-07-19 2023-10-03 不二製油株式会社 Method for producing sheet-shaped protein-containing food
WO2022075252A1 (en) * 2020-10-05 2022-04-14 不二製油グループ本社株式会社 Plant-based soup base and method for producing plant-based soup base
EP4226773A4 (en) * 2020-10-05 2024-02-21 Fuji Oil Holdings Inc Plant-based soup base and method for producing plant-based soup base
JP6963707B1 (en) * 2021-04-23 2021-11-10 キユーピー株式会社 Liquid egg substitute composition and heat coagulated product
WO2022224796A1 (en) * 2021-04-23 2022-10-27 キユーピー株式会社 Liquid egg-substitute composition and thermally coagulated product
JP2022167482A (en) * 2021-04-23 2022-11-04 キユーピー株式会社 Liquid egg substitute composition and heating-coagulated product

Similar Documents

Publication Publication Date Title
WO2012169347A1 (en) Novel application of soybean emulsion composition to soybean-derived raw material-containing food or beverage
US9101158B2 (en) Application of soybean emulsion composition to soybean-derived raw material-containing food or beverage
US9101150B2 (en) Application of reduced-fat soybean protein material to soybean-derived raw material-containing food or beverage
JP5879997B2 (en) Milk substitute composition and milk substitute food and drink using the same
Jooyandeh Soy products as healthy and functional foods
JP5397499B2 (en) Soy foods and beverages with improved flavor, and methods for producing them
CN108366592A (en) Food improver
JP2017012194A (en) Food using soybean powder and manufacturing method therefor
JP7108167B2 (en) Egg-free simulated egg food
WO2005053410A1 (en) Bread improving agent and breads containing the same
WO2005027648A1 (en) Oil-in-water emulsified composition
EP3590348B1 (en) Method for producing fresh cheese-like vegetable food product
WO2012169348A1 (en) Novel application of reduced-fat soybean protein material to soybean-derived raw material-containing food or beverage
JP2011092119A (en) Layered dough containing soy protein fermentation liquor
JP5585571B2 (en) Acid soy material and food using the same
JP5772163B2 (en) Powdered soybean material and edible composition using the same
WO2005076821A2 (en) Soy sour cream compositions and methods of preparation
JP6201308B2 (en) Bread making agent, emulsified oil composition for bread making, and bread
JP5660018B2 (en) Egg yolk substitute composition and food using the same
Chandan Dairy ingredients in bakery, snacks, sauces, dressings, processed meats, and functional foods
JP5488576B2 (en) Texture or flavor improver for manufacturing processed soybean foods and processed soybean foods
Verruck et al. Sensory Evaluation in Processed Cheese Innovation
JPH10150929A (en) Food compounded phospholipid
JP2024040708A (en) Food quality improver
JP2018171013A (en) Method for producing bean curd refuse

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12796289

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 14123837

Country of ref document: US

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 20137034124

Country of ref document: KR

Kind code of ref document: A

122 Ep: pct application non-entry in european phase

Ref document number: 12796289

Country of ref document: EP

Kind code of ref document: A1