WO2018181829A1 - Method for producing cheese-like tofu - Google Patents

Method for producing cheese-like tofu Download PDF

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Publication number
WO2018181829A1
WO2018181829A1 PCT/JP2018/013488 JP2018013488W WO2018181829A1 WO 2018181829 A1 WO2018181829 A1 WO 2018181829A1 JP 2018013488 W JP2018013488 W JP 2018013488W WO 2018181829 A1 WO2018181829 A1 WO 2018181829A1
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Prior art keywords
tofu
soybean
protein
cheese
emulsified composition
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PCT/JP2018/013488
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French (fr)
Japanese (ja)
Inventor
昌伸 柳澤
西村 隆司
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不二製油グループ本社株式会社
不二製油株式会社
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Application filed by 不二製油グループ本社株式会社, 不二製油株式会社 filed Critical 不二製油グループ本社株式会社
Priority to JP2019510198A priority Critical patent/JPWO2018181829A1/en
Publication of WO2018181829A1 publication Critical patent/WO2018181829A1/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/40Pulse curds
    • A23L11/45Soy bean curds, e.g. tofu

Definitions

  • the present invention relates to a method for producing cheese-like tofu having a cheese-like smooth texture.
  • Patent Document 1 describes that after completion of the coagulation reaction of soymilk, koji molds and enzymes are added to decompose soy protein to provide cheese-like tofu.
  • Patent Document 2 describes that cotton tofu is dehydrated and pulverized, and lipid is added by a fine powder machine to provide cream cheese-like tofu.
  • Patent Document 3 as a method for providing cheese-like tofu without using additives other than a coagulant, a method for providing cheese-like tofu by dehydrating and crushing tofu, pasting it, and solidifying by heating. It has been known.
  • Patent Document 4 discloses a soybean emulsified composition with increased lipophilic protein, but does not describe anything about adding a coagulant to the soybean emulsified composition to make a cheese-like tofu.
  • This invention makes it a subject to provide the cheese-like tofu which has a cheese-like smooth texture.
  • Patent Document 1 and Patent Document 2 since the tofu-derived raw material is added, there is a problem that it is difficult to adjust the conditions at the time of production and the work becomes complicated.
  • Patent Document 3 the tofu is dehydrated and pulverized, In order to provide cheese-like tofu by pasting and solidifying by heating, there is a problem that the process after solidification is complicated and it takes time until production.
  • the present inventors have found that the protein content per dry matter is 25% by weight or more, and the lipid content (referred to as a chloroform / methanol mixed solvent extract) is the protein content.
  • the present invention (1) For a soybean emulsified composition having a protein content per dry matter of 25% by weight or more and a lipid content (referred to as a chloroform / methanol mixed solvent extract) of 100% by weight or more based on the protein content , A method for producing cheese-like tofu, characterized by adding a coagulant after heating at a temperature of 135 ° C.
  • the soy emulsion composition has a protein content per dry matter of 25% by weight or more and a lipid content (referred to as a chloroform / methanol mixed solvent extract) of 100% by weight or more based on the protein content.
  • a cheese-like tofu having a cheese-like smooth texture can be provided by performing a high-temperature heat treatment with a heating temperature of 135 ° C. or more and a heating time of more than 2 seconds.
  • the cheese-like tofu having a cheese-like smooth texture of the present invention is characterized by containing a “soy emulsified composition” described below. Details of the soybean emulsified composition are disclosed in JP 2012-016348 A, but the soybean emulsified composition will be described below.
  • the soybean emulsified composition used for the cheese-like tofu having a smooth texture like the cheese of the present invention is derived from soybean, and among proteins, lipophilic proteins other than glycinin and ⁇ -conglycinin (or as another index)
  • the ratio of lipoxygenase protein) is particularly high, and the emulsion composition contains a large amount of neutral lipid and polar lipid. That is, the protein content per dry matter is 25% by weight or more, and the lipid content per dry matter (referred to as a chloroform / methanol mixed solvent extract) is 100% by weight or more with respect to the protein content per dry matter. It is the main feature.
  • the lipid content is measured by an ether extraction method, but the soybean emulsion composition used in the present invention contains many polar lipids that are difficult to extract with ether in addition to neutral lipids.
  • a mixed solvent of chloroform: methanol 2: 1 (volume ratio) and the amount of extract extracted at the normal pressure boiling point for 30 minutes as the total lipid amount is calculated.
  • Soxtec manufactured by FOSS can be used as the solvent extraction device.
  • the above measurement method is referred to as “chloroform / methanol mixed solvent extraction method”.
  • the soybean emulsified composition used in the present invention is characterized by containing a lipid having a value higher than the ratio of lipid content / protein content of this soybean flour, and particularly rich in polar lipids.
  • the lipid is a lipid derived from soybean as a raw material.
  • the lipid content of the soybean emulsified composition used in the present invention is 100% by weight or more, preferably 120 to 250% by weight, more preferably 120 to 200% by weight, based on the protein content per dry matter. It is characteristic that there are many. Although not essential for the constitution, when the lipid content is expressed in absolute amount, it is appropriate that it is 35% by weight or more, preferably 40% by weight or more per dry matter. If the soybean emulsified composition is one from which fibers and the like are removed, the lipid content can be increased to 50% by weight or more per dry matter.
  • the upper limit of the lipid content is not limited, but is preferably 75% by weight or less, more preferably 70% by weight or less.
  • the protein content of the soybean emulsified composition used in the present invention is 25% by weight or more, preferably 30% by weight or more per dry matter.
  • the upper limit of the protein content is not limited, but is preferably 50% by weight or less, more preferably 40% by weight or less.
  • the protein content in the present invention is measured as the amount of nitrogen by the Kjeldahl method, and is obtained by multiplying the amount of nitrogen by a nitrogen conversion factor of 6.25.
  • Each component composition of the protein of the soybean emulsified composition used in the present invention can be analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE). Hydrophobic interactions, hydrogen bonds, and intermolecular disulfide bonds between protein molecules are cleaved by the action of the surfactant SDS and the reducing agent mercaptoethanol, and negatively charged protein molecules follow their intrinsic molecular weight. By showing the electrophoretic distance, it exhibits a migration pattern characteristic of proteins. After electrophoresis, after staining the SDS gel with Coomassie Brilliant Blue (CBB), calculate the ratio of the density of bands corresponding to various protein molecules to the density of all protein bands using a densitometer. It can be obtained by the method of
  • the soybean emulsified composition used in the present invention is characterized in that the lipoxygenase protein that is hardly contained in the oil body in soybean is generally contained in a specific amount or more, and at least 4% per total protein in the soybean emulsion composition. It is contained above, preferably 5% or more.
  • the lipoxygenase protein exists in a soluble state, and therefore when extracted with water, it is extracted to the water-soluble fraction side.
  • the lipoxygenase protein remains in the insoluble fraction side because it is inactivated and insolubilized in the raw soybean by heat treatment.
  • the increase in the proportion of lipoxygenase protein in the protein not only stabilizes the emulsified state of fats and oils, but also provides a smooth texture that cannot be obtained with a normal soy protein composition mainly composed of globulin proteins. In addition, a rich flavor is added to the material.
  • lipoxygenase protein there are usually three types, L-1, L-2 and L-3, and the content can be calculated from the intensity of these bands corresponding to the lipoxygenase protein by the above-mentioned electrophoresis method.
  • Lipophilic protein The soybean emulsified composition used in the present invention is characterized in that among the types of proteins, more lipophilic proteins are contained than general soybean materials.
  • Lipophilic protein refers to a group of minor acid-precipitating soybean proteins other than glycinin (7S globulin) and ⁇ -conglycinin (11S globulin) among the major acid-precipitating soybean proteins of soybean, such as lecithin and glycolipids. It accompanies many polar lipids. Hereinafter, it may be simply abbreviated as “LP”.
  • the LP is a mixture of miscellaneous proteins, so it is difficult to identify each protein and measure the LP content precisely, but by calculating the following LCI (Lipophilic Proteins Content Index) value Can be estimated.
  • the LCI value of the protein in the soybean emulsified composition is usually 55% or more, preferably 58% or more, more preferably 60% or more, still more preferably 63% or more, most preferably 65% or more.
  • NAI 90 or higher normal undenatured soybeans
  • LP remains in the insoluble fraction side because LP is deactivated and insolubilized in the raw soybean by heat treatment.
  • the increase in the proportion of LP in the protein not only stabilizes the emulsified state of fats and oils, but also provides a physical texture that cannot be obtained with a normal soy protein composition mainly composed of globulin proteins. A rich flavor is added.
  • the soybean emulsified composition used in the present invention usually has a cream-like property, and the usual dry matter is about 20 to 30% by weight, but is not particularly limited. That is, it may be a liquid having a low viscosity by water addition, a cream having a higher viscosity by concentration processing, or a powder having been processed by powder.
  • the soybean emulsified composition used in the present invention has, for example, a water-soluble nitrogen index (Nitrogen Solubility Index, hereinafter referred to as “NSI”) of 20 to 77, preferably 20 to 70, and a lipid content per dry matter of 15% by weight or more.
  • NAI water-soluble nitrogen index
  • the suspension is solid-liquid separated to transfer neutral lipid and polar lipid to the insoluble fraction. It can be obtained by removing the water-soluble fraction containing protein and carbohydrate and recovering the insoluble fraction.
  • this production mode will be described.
  • fat-containing soybean such as full fat soybean or partially defatted soybean
  • partially defatted soybeans include those obtained by partially defatting whole fat soybeans by physical extraction treatment such as compression extraction.
  • fat is contained in about 20 to 30% by weight of dry matter in full fat soybeans, and special soybean varieties have lipids of 30% by weight or more, and are not particularly limited.
  • a material containing at least 15% by weight or more, preferably 20% by weight or more of lipid is suitable.
  • the form of the raw material may be in the form of half cracked soybeans, grits, powder.
  • soybean-emulsified composition rich in lipids used in the present invention.
  • defatted soybeans extracted with an organic solvent such as hexane and having a neutral lipid content of 1% by weight or less are not preferable because the good flavor of soybeans is impaired.
  • the NSI is usually over 90, but in the present invention, the NSI is 20 to 77, preferably 20 to 70. It is appropriate to use processed soybeans that have been treated.
  • a more preferable lower limit of NSI can be 40 or more, more preferably 41 or more, still more preferably 43 or more, and most preferably 45 or more.
  • a more preferable upper limit of NSI can be less than 75, more preferably less than 70, and a lower NSI of less than 65, alternatively less than 60, or less than 58 can be used.
  • Such processed soybeans are obtained by performing processing such as heat treatment or alcohol treatment.
  • the processing means is not particularly limited, and for example, heat treatment such as dry heat treatment, steam treatment, superheated steam treatment, microwave treatment, hydrous ethanol treatment, high pressure treatment, and combinations thereof can be used.
  • the ratio of the protein in the soybean emulsified composition tends to be high, and the lipid content to the protein is low. Moreover, miscellaneous taste such as roasting odor due to overheating tends to occur. Conversely, when the NSI is a high value of 80 or more, for example, the protein ratio in the soybean emulsified composition decreases, and the lipid recovery rate from soybean tends to decrease. In addition, the flavor has a strong blue odor.
  • the treatment conditions are influenced by the production environment, so it cannot be said unconditionally, but the NSI of processed soybeans is used for 5 to 10 minutes using superheated steam at approximately 120 to 250 ° C.
  • the processing conditions may be selected as appropriate so that the value falls within the above range, and no particular difficulty is required for the processing. For convenience, commercially available soybeans with NSI processed in the above range can also be used.
  • NSI can be expressed as a ratio (% by weight) of water-soluble nitrogen (crude protein) in the total amount of nitrogen based on a predetermined method.
  • NSI is a value measured based on the following method. . That is, 100 ml of water is added to 2.0 g of a sample, followed by stirring and extraction at 40 ° C. for 60 minutes, followed by centrifugation at 1400 ⁇ g for 10 minutes to obtain supernatant 1. 100 ml of water is added again to the remaining precipitate, followed by stirring and extraction at 40 ° C. for 60 minutes, and centrifugation at 1400 ⁇ g for 10 minutes to obtain supernatant 2.
  • Supernatant 1 and supernatant 2 are combined, and water is further added to make 250 ml.
  • the nitrogen content of the filtrate is measured by Kjeldahl method.
  • the nitrogen content in the sample is measured by the Kjeldahl method, and the ratio of the nitrogen recovered as filtrate (water-soluble nitrogen) to the total nitrogen in the sample is expressed as weight%, which is NSI.
  • the processed soybean is subjected to a tissue destruction treatment such as dry or wet pulverization, crushing, and pressure bias in advance before water extraction.
  • tissue destruction treatment it may be swollen in advance by water immersion or steaming, thereby reducing the energy required for tissue destruction, and eluting and removing components with unpleasant taste such as whey protein and oligosaccharides, as well as water retention
  • the extraction ratio of globulin proteins (especially glycinin and ⁇ -conglycinin) having a high ability and gelling ability to the total protein, that is, the transfer ratio to the water-soluble fraction can be further increased.
  • Water extraction is performed by adding 3 to 20 times, preferably 4 to 15 times, the amount of water added to fat-containing soybeans and suspending the fat-containing soybeans.
  • the higher the rate of hydrolysis the higher the extraction rate of the water-soluble component and the better the separation. However, if it is too high, concentration is required and costs increase. Further, when the extraction process is repeated twice or more, the extraction rate of the water-soluble component can be further increased.
  • the extraction temperature is not particularly limited. However, the higher the extraction rate of the water-soluble component, the more easily the oil and fat are solubilized, and the lower the lipid of the soybean emulsified composition. The following should be done. Alternatively, it can be carried out in the range of 5 to 80 ° C., preferably 50 to 75 ° C.
  • the lower limit can be adjusted to pH 6 or higher, pH 6.3 or higher, or pH 6.5 or higher.
  • the upper limit may be adjusted to pH 9 or lower, pH 8 or lower, or pH 7 or lower from the viewpoint of increasing lipid separation efficiency.
  • the suspension of fat-containing soybeans is subjected to solid-liquid separation by centrifugation, filtration or the like.
  • the suspension of fat-containing soybeans is subjected to solid-liquid separation by centrifugation, filtration or the like.
  • not only neutral lipids but also most of the lipids including polar lipids are not eluted in the water extract, but transferred to the insolubilized protein or dietary fiber to make the precipitation side (insoluble fraction). is important. Specifically, 70% by weight or more of the lipid of the fat-containing soybean is transferred to the precipitation side.
  • lipid in soy milk a small amount of lipid is also eluted on the supernatant side during extraction, but it is not finely emulsified like the lipid in soy milk, and it is centrifuged at 15,000 xg or less, or about 5,000 xg or less. In this respect, it is preferable to use a centrifuge. Depending on the equipment used, the centrifugal separator can be used for ultracentrifugation of 100,000 xg or more. In the case of the soybean emulsion composition used in the present invention, the centrifugal separator is not used. Is possible. It is also possible to add a demulsifier during or after water extraction to promote lipid separation from soy milk. The demulsifier is not particularly limited, but for example, the demulsifier disclosed in Patent Document 2 is used. do it. However, the preparation of the soybean emulsified composition used in the present invention can be carried out without using a demulsifier.
  • the obtained insoluble fractions (1) and (3) can be used as they are, or, if necessary, through a concentration step, a heat sterilization step, a powdering step, etc. to obtain a soybean emulsion composition used in the present invention.
  • the obtained insoluble fraction contains dietary fiber, for example, when it is the fraction of (3) or (1) and (3) above, water is added if necessary, and a high-pressure homogenizer or jet cooker heater After homogenization by means of, etc., through the process of further solid-liquid separation of the homogenized liquid and collecting the supernatant, dietary fiber (ocara) can also be removed, and the rich flavor is further concentrated A soybean emulsified composition can be obtained. Proteins can be more easily extracted by adding a heat treatment step, an alkali treatment step or the like, if necessary, before or after the homogenization. In this case, the dietary fiber content per dry matter is 10% by weight or less, and more preferably 5% by weight or less. In the present invention, the dietary fiber content can be measured by the enzyme-weight method (modified Prosky method) according to the “Fifth Supplement Japanese Food Standard Ingredient Table” (Ministry of Education, Culture, Sports, Science and Technology, 2005).
  • soybean emulsified composition used in the present invention contains a lipid (neutral lipid and polar lipid) and protein in a specific range, has a particularly high LP content of protein, and optionally contains fiber.
  • the natural taste of soy is concentrated, and there is little or no unpleasant taste such as blue odor, astringent taste, astringency, etc. It has a flavor.
  • water and fat to normal soy flour and isolated soy protein to make an emulsified composition similar to the soy emulsified composition, but adjust the lipoxygenase protein content or LCI value to an equivalent level It is difficult.
  • the soybean emulsified composition prepared by this technique is characterized by remarkably good flavor and high suitability for use as a food material compared to such an assembled product.
  • High temperature heat treatment In the present invention, it is important to perform a high-temperature heat treatment on at least the soybean emulsion composition.
  • the protein of the soybean emulsified composition is denatured and becomes a tofu having a cheese-like smooth texture.
  • conventionally known devices such as various heat exchangers such as a plate heat exchanger, a tube heat exchanger, a scraping heat exchanger, and a steam injection heat exchanger, and a batch retort kettle can be used. These devices may be used in any combination.
  • the heat treatment conditions are important, and it is difficult to perform the heat conditions only for the purpose of sterilization. That is, it is important that the heating temperature is at least 135 ° C. or more, more preferably 138 to 145 ° C., and further preferably 138 to 142 ° C. When the heating temperature is low, the texture of tofu may not have a cheese-like smooth texture. Furthermore, it is important that the heating time be at least 2 seconds, preferably 2 to 40 seconds, more preferably 4 to 20 seconds. When the heating time is short, the texture of the obtained tofu may not have a cheese-like smooth texture.
  • the heat-treated soybean emulsion composition can be homogenized with a homogenizer or the like, although not necessarily essential.
  • a homogenizer for example, when a high-pressure homogenizer is used, a pressure of 3 to 15 MPa is appropriate.
  • a coagulant is added to the soybean emulsified composition used in the present invention or a mixture of the soy emulsified composition and soy milk, and the protein is coagulated by heating to produce a cheese-like tofu having a cheese-like smooth texture.
  • Cheese-like smooth texture refers to the unique smooth texture of soft cheeses. Specific examples of the soft cheeses include cream cheese, mascarpone cheese, quark cheese, and fromage blanc, but are not limited thereto.
  • a coagulant is added to the soybean emulsified composition used in the present invention or a mixture of the soybean emulsified composition and soy milk, and the protein is coagulated by heating to produce a cheese-like tofu having a cheese-like smooth texture. be able to.
  • Cheese-like tofu can be produced by a known production method and can be produced without any particular difficulty.
  • bittern magnesium chloride
  • calcium chloride calcium sulfate, GDL and the like can be used as the coagulant.
  • the coagulant is preferably added after the soybean emulsion composition is subjected to a high-temperature heat treatment at a heating temperature of 135 ° C. or more and a heating time of 2 seconds or more.
  • a cheese-like smooth texture may not be obtained.
  • the amount of the coagulant added is not particularly limited, but may be, for example, 0.1 to 5% by weight, preferably 0.3 to 3% by weight, based on the soybean emulsified composition and the dried soybean milk.
  • the soybean emulsified composition may be used as a raw material, and a small amount of the coagulant may be added.
  • the cheese-like tofu obtained has a texture that is very creamy and melts in the mouth, similar to soft cheeses, and has a texture that cannot be obtained with conventional smooth tofu. It has become.
  • the various additives currently added in general manufacture can be added to cheese-like tofu.
  • the various additives currently added in general manufacture can be added alone or in combination.
  • the coagulant salts, oligosaccharides, polysaccharides, starches, proteins and the like can be added alone or in combination.
  • soybean emulsified composition as a raw material for deep-fried food, deep-fried food with a smooth texture like cheese can be obtained.
  • soybean emulsified composition was prepared based on the description in Example 1 of JP2012-016348A.
  • the resulting soybean emulsified composition had a dry matter content of 30.6%, a protein amount and a lipid amount per dry matter of 32.2% and 43.0%, respectively, and an LCI value of 67%.
  • the texture was evaluated as 1 for a hard texture like tofu and 5 for a smooth texture like cheese (passed 4.0 or higher).
  • the flavor was evaluated as 1 with a rich and refreshing flavor, and 5 with a rich and rich flavor (4.0 or higher passed). The results are shown in Table 2.
  • the tofu had a cheese-like creamy texture at a heating temperature of 135 ° C. or higher.
  • the texture was evaluated as 1 for a hard texture like tofu and 5 for a smooth texture like cheese (passed 4.0 or higher).
  • the flavor was evaluated as 1 with a rich and refreshing flavor, and 5 with a rich and rich flavor (4.0 or higher passed). The results are shown in Table 3.
  • Example 6 As shown in Examples 5 to 9, tofu having a cheese-like creamy texture was obtained at a heating time of 2 seconds or more. Especially in 4 to 20 seconds, a very smooth cheese-like texture was obtained. (Example 6, Example 7)
  • the soybean emulsified composition obtained in Production Example 1 is fixed at a heating temperature of 130 ° C. with a plate-type UHT sterilizer (manufactured by Powerpoint International), and the heating temperature is 2 seconds, 4 seconds, 20 seconds, 40 High-temperature heat treatment was performed in seconds and 60 seconds.
  • a plate-type UHT sterilizer manufactured by Powerpoint International
  • High-temperature heat treatment was performed in seconds and 60 seconds.
  • To 100 parts of the heat-treated soybean emulsified composition 3.4 parts of Shiota nigari sachet (Ako Kasei Co., Ltd.) was added, steamed in a steamer for 7 minutes 30 seconds, and cooled with ice. Sensory evaluation was performed on the produced tofu and evaluated in five stages.
  • the texture was evaluated as 1 for a hard texture like tofu and 5 for a smooth texture like cheese (passed 4.0 or higher).
  • the flavor was evaluated as 1 with a rich and refreshing flavor, and 5 with a rich and rich flavor (4.0 or higher passed). The results are shown in Table 4.
  • the soybean emulsified composition characterized in that the lipophilic protein is contained in a larger amount than a general soybean material, by performing a high-temperature heat treatment under specific conditions, it is different from the conventional bean corrosion feeling. It becomes possible to obtain a cheese-like tofu having a smooth texture like cheese. This cheese-like tofu matches consumer needs for diversifying the tofu food scene and is expected to greatly contribute to the activation of the tofu market.

Abstract

The purpose of the present invention is to provide a method for producing a tofu (soybean curd) which has a smooth cheese-like texture different from the texture of conventional tofus. A tofu having a smooth cheese-like texture different from the texture of conventional tofus can be obtained by subjecting an emulsified soybean composition, said emulsified soybean composition having a protein content of 25 wt% or more on a dry basis and a lipid content (a content as an extract obtained by using a chloroform/methanol solvent mixture) of 100 wt% or more relative to the protein content, to a high-temperature heating treatment at a heating temperature of 135°C or higher for a heating time of 2 seconds or longer.

Description

チーズ様豆腐の製造方法Cheese-like tofu manufacturing method
 本発明はチーズ様の滑らかな食感を有するチーズ様豆腐の製造方法に関するものである。 The present invention relates to a method for producing cheese-like tofu having a cheese-like smooth texture.
 近年、食生活の洋風化により、チーズ類の消費が拡大している一方、豆腐類の消費は低迷している。また、消費者ニーズとして、チーズ類の風味や食感が好まれる傾向が強くなっているため、豆腐類の消費拡大のためにも、従来の豆腐と異なり、食感を変化させチーズ様食感などを付与したチーズ様豆腐が要望されている。チーズ様食感を有する豆腐の製造方法として、豆腐由来の原料以外に油脂や酵素などを添加する方法があげられる。 In recent years, consumption of cheese has increased due to westernization of eating habits, while consumption of tofu has been sluggish. In addition, as consumer needs tend to favor the flavor and texture of cheeses, to increase consumption of tofu, unlike conventional tofu, the texture changes and cheese-like texture There is a demand for cheese-like tofu that has been added. As a method for producing tofu having a cheese-like texture, there may be mentioned a method of adding fats and oils, enzymes and the like in addition to raw materials derived from tofu.
 特許文献1では、豆乳の凝固反応終了後に麹菌や酵素を添加し、大豆タンパク質を分解させ、チーズ様豆腐を提供することが記載されている。
 特許文献2では、木綿豆腐を脱水・粉砕し、微粉機により脂質を添加しクリームチーズ様豆腐を提供することが記載されている。一方、特許文献3では、凝固剤以外の添加物を使用することなくチーズ様豆腐を提供する方法として、豆腐を脱水・粉砕し、ペースト状にし、加熱凝固することによりチーズ様豆腐を適供する方法が知られている。
 特許文献4には脂質親和性蛋白質が増加された大豆乳化組成物について開示しているが、該大豆乳化組成物に凝固剤を加えチーズ様豆腐にすることについて何ら記載されていない。
Patent Document 1 describes that after completion of the coagulation reaction of soymilk, koji molds and enzymes are added to decompose soy protein to provide cheese-like tofu.
Patent Document 2 describes that cotton tofu is dehydrated and pulverized, and lipid is added by a fine powder machine to provide cream cheese-like tofu. On the other hand, in Patent Document 3, as a method for providing cheese-like tofu without using additives other than a coagulant, a method for providing cheese-like tofu by dehydrating and crushing tofu, pasting it, and solidifying by heating. It has been known.
Patent Document 4 discloses a soybean emulsified composition with increased lipophilic protein, but does not describe anything about adding a coagulant to the soybean emulsified composition to make a cheese-like tofu.
特開2004-141129号公報JP 2004-141129 A 特開2013-215181号公報JP 2013-215181 A 特開2009-131217号公報JP 2009-131217 A 特開2012-016348号公報JP 2012-016348 A
 本発明は、チーズ様の滑らかな食感を有するチーズ様豆腐を提供することを課題とする。 This invention makes it a subject to provide the cheese-like tofu which has a cheese-like smooth texture.
 特許文献1や特許文献2の方法では、豆腐由来の原料を加えるため、製造時の条件調整が難しく、作業が煩雑となる問題があり、特許文献3の方法では、豆腐を脱水・粉砕し、ペースト状にし、加熱凝固することによりチーズ様豆腐を適供するため、凝固後の工程が煩雑であり生産まで時間がかかるという問題があった。
 本発明者らは、上記課題を解決すべく鋭意研究を行ったところ乾物あたりの蛋白質含量が25重量%以上、脂質含量(クロロホルム/メタノール混合溶媒抽出物としての含量をいう。)が蛋白質含量に対して100重量%以上である大豆乳化組成物に対して、加熱温度135℃以上、加熱時間2秒以上を超える高温加熱処理を施すことにより、チーズ様な滑らかな食感につながるという知見を得、上記課題を解決するに至った。
In the methods of Patent Document 1 and Patent Document 2, since the tofu-derived raw material is added, there is a problem that it is difficult to adjust the conditions at the time of production and the work becomes complicated. In the method of Patent Document 3, the tofu is dehydrated and pulverized, In order to provide cheese-like tofu by pasting and solidifying by heating, there is a problem that the process after solidification is complicated and it takes time until production.
As a result of intensive research to solve the above problems, the present inventors have found that the protein content per dry matter is 25% by weight or more, and the lipid content (referred to as a chloroform / methanol mixed solvent extract) is the protein content. On the other hand, we obtained knowledge that a soybean emulsified composition with a weight of 100% by weight or more is heated at a temperature of 135 ° C or higher and heated for more than 2 seconds, resulting in a cheese-like smooth texture. The above problems have been solved.
 すなわち、本発明は、
(1)乾物あたりの蛋白質含量が25重量%以上、脂質含量(クロロホルム/メタノール混合溶媒抽出物としての含量をいう。)が蛋白質含量に対して100重量%以上である大豆乳化組成物 に対して、加熱温度135℃以上、加熱時間2秒以上の高温加熱処理を施した後、凝固剤を加えることを特徴とするチーズ様豆腐の製造方法、 
(2)大豆乳化組成物が豆乳であること特徴とする、(1)記載のチーズ様豆腐の製造方法、
(3)加熱温度が135~145℃であることを特徴とする、(1)記載のチーズ様豆腐の製造方法、
(4)加熱時間が4~20秒であることを特徴とする、(1)記載のチーズ様豆腐の製造方法、
である。
That is, the present invention
(1) For a soybean emulsified composition having a protein content per dry matter of 25% by weight or more and a lipid content (referred to as a chloroform / methanol mixed solvent extract) of 100% by weight or more based on the protein content , A method for producing cheese-like tofu, characterized by adding a coagulant after heating at a temperature of 135 ° C. or higher and a heating time of 2 seconds or more,
(2) The method for producing cheese-like tofu according to (1), wherein the soybean emulsified composition is soy milk,
(3) The method for producing cheese-like tofu according to (1), wherein the heating temperature is 135 to 145 ° C.,
(4) The method for producing cheese-like tofu according to (1), wherein the heating time is 4 to 20 seconds,
It is.
 本発明によれば、乾物あたりの蛋白質含量が25重量%以上、脂質含量(クロロホルム/メタノール混合溶媒抽出物としての含量をいう。)が蛋白質含量に対して100重量%以上である大豆乳化組成物に対して、加熱温度135℃以上、加熱時間2秒以上を超える高温加熱処理を施すことによりチーズ様な滑らかな食感を有するチーズ様豆腐を提供することができる。 According to the present invention, the soy emulsion composition has a protein content per dry matter of 25% by weight or more and a lipid content (referred to as a chloroform / methanol mixed solvent extract) of 100% by weight or more based on the protein content. On the other hand, a cheese-like tofu having a cheese-like smooth texture can be provided by performing a high-temperature heat treatment with a heating temperature of 135 ° C. or more and a heating time of more than 2 seconds.
(大豆乳化組成物)
 本発明のチーズ様の滑らかな食感を有するチーズ様豆腐は、下記に説明する「大豆乳化組成物」を含有することが特徴である。大豆乳化組成物の詳細は、特開2012-016348号に開示されるものであるが、以下、該大豆乳化組成物について説明する。
(Soy emulsified composition)
The cheese-like tofu having a cheese-like smooth texture of the present invention is characterized by containing a “soy emulsified composition” described below. Details of the soybean emulsified composition are disclosed in JP 2012-016348 A, but the soybean emulsified composition will be described below.
 本発明のチーズ様の滑らかな食感を有するチーズ様豆腐に用いられる大豆乳化組成物は、大豆を由来とし、蛋白質のうち、グリシニンやβ-コングリシニン以外の脂質親和性蛋白質(あるいは別の指標としてリポキシゲナーゼ蛋白質)の割合が特に高く、中性脂質及び極性脂質を多く含む乳化組成物である。すなわち、乾物あたりの蛋白質含量が25重量%以上、乾物あたりの脂質含量(クロロホルム/メタノール混合溶媒抽出物としての含量をいう。)が乾物あたりの蛋白質含量に対して100重量%以上であることを主要な特徴とするものである。 The soybean emulsified composition used for the cheese-like tofu having a smooth texture like the cheese of the present invention is derived from soybean, and among proteins, lipophilic proteins other than glycinin and β-conglycinin (or as another index) The ratio of lipoxygenase protein) is particularly high, and the emulsion composition contains a large amount of neutral lipid and polar lipid. That is, the protein content per dry matter is 25% by weight or more, and the lipid content per dry matter (referred to as a chloroform / methanol mixed solvent extract) is 100% by weight or more with respect to the protein content per dry matter. It is the main feature.
(脂質)
 一般に脂質含量はエーテル抽出法で測定されるが、本発明に用いられる大豆乳化組成物中には中性脂質の他にエーテルで抽出されにくい極性脂質も多く含まれるため、本発明における脂質含量は、クロロホルム:メタノールが2:1(体積比)の混合溶媒を用い、常圧沸点において30分間抽出された抽出物量を総脂質量として、脂質含量を算出した値とする。溶媒抽出装置としてはFOSS社製の「ソックステック」を用いることができる。なお上記の測定法は「クロロホルム/メタノール混合溶媒抽出法」と称するものとする。
(Lipid)
In general, the lipid content is measured by an ether extraction method, but the soybean emulsion composition used in the present invention contains many polar lipids that are difficult to extract with ether in addition to neutral lipids. Using a mixed solvent of chloroform: methanol 2: 1 (volume ratio) and the amount of extract extracted at the normal pressure boiling point for 30 minutes as the total lipid amount, the lipid content is calculated. As the solvent extraction device, “Soxtec” manufactured by FOSS can be used. The above measurement method is referred to as “chloroform / methanol mixed solvent extraction method”.
 本発明に用いられる大豆乳化組成物は、この大豆粉の脂質含量/蛋白質含量の比よりも高い値の脂質を含み、特に極性脂質に富むことが特徴である。該脂質は原料となる大豆に由来する脂質である。 The soybean emulsified composition used in the present invention is characterized by containing a lipid having a value higher than the ratio of lipid content / protein content of this soybean flour, and particularly rich in polar lipids. The lipid is a lipid derived from soybean as a raw material.
 本発明に用いられる大豆乳化組成物の脂質含量は、乾物あたりの蛋白質含量に対して100重量%以上、好ましくは120~250重量%、さらに好ましくは120~200重量%であり、蛋白質よりも脂質が多いことが特徴である。また構成に必須ではないが、脂質含量を絶対量で表す場合、乾物あたり35重量%以上、好ましくは40重量%以上であるのが適当である。大豆乳化組成物を繊維質等が除去されたものとすれば脂質含量を乾物あたり50重量%以上にもすることができる。また脂質含量の上限は限定されないが、好ましくは75重量%以下、より好ましくは70重量%以下である。 The lipid content of the soybean emulsified composition used in the present invention is 100% by weight or more, preferably 120 to 250% by weight, more preferably 120 to 200% by weight, based on the protein content per dry matter. It is characteristic that there are many. Although not essential for the constitution, when the lipid content is expressed in absolute amount, it is appropriate that it is 35% by weight or more, preferably 40% by weight or more per dry matter. If the soybean emulsified composition is one from which fibers and the like are removed, the lipid content can be increased to 50% by weight or more per dry matter. The upper limit of the lipid content is not limited, but is preferably 75% by weight or less, more preferably 70% by weight or less.
(蛋白質)
 本発明に用いられる大豆乳化組成物の蛋白質含量は乾物あたり25重量%以上、好ましくは30重量%以上である。また蛋白質含量の上限は限定されないが、好ましくは50重量%以下、より好ましくは40重量%以下である。
(protein)
The protein content of the soybean emulsified composition used in the present invention is 25% by weight or more, preferably 30% by weight or more per dry matter. The upper limit of the protein content is not limited, but is preferably 50% by weight or less, more preferably 40% by weight or less.
○蛋白質含量の分析
 本発明における蛋白質含量はケルダール法により窒素量として測定し、該窒素量に6.25の窒素換算係数を乗じて求めるものとする。
Analysis of protein content The protein content in the present invention is measured as the amount of nitrogen by the Kjeldahl method, and is obtained by multiplying the amount of nitrogen by a nitrogen conversion factor of 6.25.
○蛋白質の各成分の組成分析
 本発明に用いられる大豆乳化組成物の蛋白質の各成分組成はSDSポリアクリルアミドゲル電気泳動(SDS-PAGE)により分析することができる。
 界面活性剤であるSDSと還元剤であるメルカプトエタノールの作用によって蛋白質分子間の疎水性相互作用、水素結合、分子間のジスルフィド結合が切断され、マイナスに帯電した蛋白質分子は固有の分子量に従った電気泳動距離を示ことにより、蛋白質に特徴的な泳動パターンを呈する。電気泳動後に色素であるクマシーブリリアントブルー(CBB)にてSDSゲルを染色した後に、デンシトメーターを用い、全蛋白質のバンドの濃さに対する各種蛋白質分子に相当するバンドの濃さが占める割合を算出する方法により求めることができる。
-Composition analysis of each component of protein Each component composition of the protein of the soybean emulsified composition used in the present invention can be analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE).
Hydrophobic interactions, hydrogen bonds, and intermolecular disulfide bonds between protein molecules are cleaved by the action of the surfactant SDS and the reducing agent mercaptoethanol, and negatively charged protein molecules follow their intrinsic molecular weight. By showing the electrophoretic distance, it exhibits a migration pattern characteristic of proteins. After electrophoresis, after staining the SDS gel with Coomassie Brilliant Blue (CBB), calculate the ratio of the density of bands corresponding to various protein molecules to the density of all protein bands using a densitometer. It can be obtained by the method of
(リポキシゲナーゼ蛋白質)
 本発明に用いられる大豆乳化組成物は、一般に大豆中のオイルボディにはほとんど含まれないリポキシゲナーゼ蛋白質が特定量以上含まれることが大きな特徴であり、大豆乳化組成物中の全蛋白質あたり少なくとも4%以上含有し、好ましくは5%以上含有するものである。
 通常の未変性(NSI 90以上)の大豆を原料とした場合ではリポキシゲナーゼ蛋白質は可溶性の状態で存在するため、水抽出すると水溶性画分側へ抽出される。一方、本発明ではリポキシゲナーゼ蛋白質が原料大豆中において加熱処理によって失活され不溶化しているため、不溶性画分側に残る。
 蛋白質中におけるリポキシゲナーゼ蛋白質の割合が高まることによって油脂の乳化状態が安定化されるばかりでなく、グロブリン蛋白質を主体とした通常の大豆蛋白質組成では得られない滑らかな物性の食感を得ることができ、また素材にコクのある風味が付与される。
(Lipoxygenase protein)
The soybean emulsified composition used in the present invention is characterized in that the lipoxygenase protein that is hardly contained in the oil body in soybean is generally contained in a specific amount or more, and at least 4% per total protein in the soybean emulsion composition. It is contained above, preferably 5% or more.
When normal undenatured (NSI 90 or higher) soybeans are used as the raw material, the lipoxygenase protein exists in a soluble state, and therefore when extracted with water, it is extracted to the water-soluble fraction side. On the other hand, in the present invention, the lipoxygenase protein remains in the insoluble fraction side because it is inactivated and insolubilized in the raw soybean by heat treatment.
The increase in the proportion of lipoxygenase protein in the protein not only stabilizes the emulsified state of fats and oils, but also provides a smooth texture that cannot be obtained with a normal soy protein composition mainly composed of globulin proteins. In addition, a rich flavor is added to the material.
 リポキシゲナーゼ蛋白質の場合は通常L-1、L-2、L-3の3種類が存在し、上記の電気泳動法により、リポキシゲナーゼ蛋白質に相当するこれらのバンドの濃さから含量を算出できる。 In the case of lipoxygenase protein, there are usually three types, L-1, L-2 and L-3, and the content can be calculated from the intensity of these bands corresponding to the lipoxygenase protein by the above-mentioned electrophoresis method.
(脂質親和性蛋白質)
 本発明に用いられる大豆乳化組成物は、蛋白質の種類の中では脂質親和性蛋白質(Lipophilic Proteins)が一般の大豆素材より多く含まれることが特徴である。脂質親和性蛋白質は、大豆の主要な酸沈殿性大豆蛋白質の内、グリシニン(7Sグロブリン)とβ-コングリシニン(11Sグロブリン)以外のマイナーな酸沈殿性大豆蛋白質群をいい、レシチンや糖脂質などの極性脂質を多く随伴するものである。以下、単に「LP」と略記することがある。
 LPは雑多な蛋白質が混在したものであるが故、各々の蛋白質を全て特定し、LPの含量を厳密に測定することは困難であるが、下記LCI(Lipophilic Proteins Content Index)値を求めることにより推定することができる。これによれば、大豆乳化組成物中の蛋白質のLCI値は通常55%以上であり、好ましくは58%以上であり、より好ましくは60%以上であり、さらに好ましくは63%以上、最も好ましくは65%以上である。
 通常の未変性(NSI 90以上)の大豆を原料とした場合ではLPは可溶性の状態で存在するため、水抽出すると水溶性画分側へ抽出される。一方、本発明に用いられる大豆乳化組成物の場合、LPが原料大豆中において加熱処理によって失活され不溶化しているため、不溶性画分側に残る。
 蛋白質中におけるLPの割合が高まることによって油脂の乳化状態が安定化されるばかりでなく、グロブリン蛋白質を主体とした通常の大豆蛋白質組成では得られない物性の食感を得ることができ、また素材にコクのある風味が付与される。
(Lipophilic protein)
The soybean emulsified composition used in the present invention is characterized in that among the types of proteins, more lipophilic proteins are contained than general soybean materials. Lipophilic protein refers to a group of minor acid-precipitating soybean proteins other than glycinin (7S globulin) and β-conglycinin (11S globulin) among the major acid-precipitating soybean proteins of soybean, such as lecithin and glycolipids. It accompanies many polar lipids. Hereinafter, it may be simply abbreviated as “LP”.
LP is a mixture of miscellaneous proteins, so it is difficult to identify each protein and measure the LP content precisely, but by calculating the following LCI (Lipophilic Proteins Content Index) value Can be estimated. According to this, the LCI value of the protein in the soybean emulsified composition is usually 55% or more, preferably 58% or more, more preferably 60% or more, still more preferably 63% or more, most preferably 65% or more.
When normal undenatured soybeans (NSI 90 or higher) are used as raw materials, LP exists in a soluble state, so when extracted with water, it is extracted to the water-soluble fraction. On the other hand, in the soybean emulsified composition used in the present invention, LP remains in the insoluble fraction side because LP is deactivated and insolubilized in the raw soybean by heat treatment.
The increase in the proportion of LP in the protein not only stabilizes the emulsified state of fats and oils, but also provides a physical texture that cannot be obtained with a normal soy protein composition mainly composed of globulin proteins. A rich flavor is added.
〔LP含量の推定・LCI値の測定方法〕
(a) 各蛋白質中の主要な蛋白質として、7Sはαサブユニット及びα'サブユニット(α+α')、11Sは酸性サブユニット(AS)、LPは34kDa蛋白質及びリポキシゲナーゼ蛋白質(P34+Lx)を選択し、SDS-PAGEにより選択された各蛋白質の染色比率を求める。電気泳動は表1の条件で行うことが出来る。
(b) X(%)=(P34+Lx)/{(P34+Lx)+(α+α’)+AS}×100(%)を求める。
(c) 低変性脱脂大豆から調製された分離大豆蛋白のLP含量を加熱殺菌前に上記方法1,2の分画法により測定すると凡そ38%となることから、X=38(%)となるよう(P34+Lx)に補正係数k*=6を掛ける。
(d) すなわち、以下の式によりLP推定含量(Lipophilic Proteins Content Index、以下「LCI」と略する。)を算出する。
[LP content estimation / LCI value measurement method]
(a) As the main protein in each protein, 7S selects α subunit and α ′ subunit (α + α ′), 11S selects acidic subunit (AS), LP selects 34 kDa protein and lipoxygenase protein (P34 + Lx), Obtain the staining ratio of each protein selected by SDS-PAGE. Electrophoresis can be performed under the conditions shown in Table 1.
(b) X (%) = (P34 + Lx) / {(P34 + Lx) + (α + α ′) + AS} × 100 (%) is obtained.
(c) Since the LP content of the separated soybean protein prepared from low-denatured defatted soybean is measured by the fractionation method of methods 1 and 2 before heat sterilization, it is about 38%, so X = 38 (%). (P34 + Lx) is multiplied by the correction coefficient k * = 6.
(d) That is, the estimated LP content (Lipophilic Proteins Content Index, hereinafter abbreviated as “LCI”) is calculated by the following equation.
(表1)
Figure JPOXMLDOC01-appb-I000001

Figure JPOXMLDOC01-appb-I000002
(Table 1)
Figure JPOXMLDOC01-appb-I000001

Figure JPOXMLDOC01-appb-I000002
(乾物含量)
 本発明に用いられる大豆乳化組成物は通常生クリーム様の性状であり、通常の乾物(dry matter)は20~30重量%程度であるが、特に限定されるものではない。すなわち加水により低粘度の液状としたものや、濃縮加工されてより高粘度のクリーム状としたものであってもよく、また粉末加工されて粉末状としたものであってもよい。
(Dry matter content)
The soybean emulsified composition used in the present invention usually has a cream-like property, and the usual dry matter is about 20 to 30% by weight, but is not particularly limited. That is, it may be a liquid having a low viscosity by water addition, a cream having a higher viscosity by concentration processing, or a powder having been processed by powder.
(大豆乳化組成物の製造態様)
 本発明に用いられる大豆乳化組成物は、例えば水溶性窒素指数(Nitrogen Solubility Index、以下「NSI」と称する。)が20~77、好ましくは20~70、乾物あたりの脂質含量が15重量%以上の全脂大豆などの含脂大豆に対して、加水して懸濁液を調製する工程の後、該懸濁液を固液分離し、中性脂質及び極性脂質を不溶性画分に移行させて、蛋白質及び糖質を含む水溶性画分を除去し、不溶性画分を回収することにより得ることができる。以下、該製造態様について示す。
(Production aspect of soybean emulsified composition)
The soybean emulsified composition used in the present invention has, for example, a water-soluble nitrogen index (Nitrogen Solubility Index, hereinafter referred to as “NSI”) of 20 to 77, preferably 20 to 70, and a lipid content per dry matter of 15% by weight or more. After the step of preparing a suspension by adding water to a fat-containing soybean such as whole fat soybean, the suspension is solid-liquid separated to transfer neutral lipid and polar lipid to the insoluble fraction. It can be obtained by removing the water-soluble fraction containing protein and carbohydrate and recovering the insoluble fraction. Hereinafter, this production mode will be described.
・原料大豆及びその加工
 大豆乳化組成物の原料である大豆としては、全脂大豆あるいは部分脱脂大豆等の含脂大豆を用いる。部分脱脂大豆としては、全脂大豆を圧搾抽出等の物理的な抽出処理により部分的に脱脂したものが挙げられる。一般に全脂大豆中には脂質が乾物あたり約20~30重量%程度含まれ、特殊な大豆品種については脂質が30重量%以上のものもあり、特に限定されないが、用いる含脂大豆としては、少なくとも脂質を15重量以上、好ましくは20重量%以上含むものが適当である。原料の形態は、半割れ大豆、グリッツ、粉末の形状でありうる。
 過度に脱脂され脂質含量が少なすぎると本発明に用いられる脂質に富む大豆乳化組成物を得ることが困難となる。特にヘキサン等の有機溶媒で抽出され、中性脂質の含量が1重量%以下となった脱脂大豆は、大豆の良い風味が損なわれ好ましくない。
-Raw soybean and its processing As soybean which is the raw material of the soybean emulsified composition, fat-containing soybean such as full fat soybean or partially defatted soybean is used. Examples of partially defatted soybeans include those obtained by partially defatting whole fat soybeans by physical extraction treatment such as compression extraction. Generally, fat is contained in about 20 to 30% by weight of dry matter in full fat soybeans, and special soybean varieties have lipids of 30% by weight or more, and are not particularly limited. A material containing at least 15% by weight or more, preferably 20% by weight or more of lipid is suitable. The form of the raw material may be in the form of half cracked soybeans, grits, powder.
If it is excessively defatted and the lipid content is too small, it will be difficult to obtain a soybean-emulsified composition rich in lipids used in the present invention. In particular, defatted soybeans extracted with an organic solvent such as hexane and having a neutral lipid content of 1% by weight or less are not preferable because the good flavor of soybeans is impaired.
 上記含脂大豆は天然の状態では蛋白質の多くが未変性で可溶性の状態にあり、NSIとしては通常90を超えるが、本発明においては、NSIが20~77好ましくは20~70になるよう加工処理を施した加工大豆を用いるのが適当である。より好ましいNSIの下限値は40以上、より好ましくは41以上、さらに好ましくは43以上、最も好ましくは45以上とすることができる。より好ましいNSIの上限値は75未満、より好ましくは70未満とすることができ、またさらに65未満、あるいは60未満、あるいは58未満の低NSIのものを用いることができる。
 そのような加工大豆は、加熱処理やアルコール処理等の加工処理を行って得られる。加工処理の手段は特に限定されないが、例えば乾熱処理、水蒸気処理、過熱水蒸気処理、マイクロ波処理等による加熱処理や、含水エタノール処理、高圧処理、およびこれらの組み合わせ等が利用できる。
In the above-mentioned fat-containing soybean, most of the protein is in an unmodified and soluble state in the natural state, and the NSI is usually over 90, but in the present invention, the NSI is 20 to 77, preferably 20 to 70. It is appropriate to use processed soybeans that have been treated. A more preferable lower limit of NSI can be 40 or more, more preferably 41 or more, still more preferably 43 or more, and most preferably 45 or more. A more preferable upper limit of NSI can be less than 75, more preferably less than 70, and a lower NSI of less than 65, alternatively less than 60, or less than 58 can be used.
Such processed soybeans are obtained by performing processing such as heat treatment or alcohol treatment. The processing means is not particularly limited, and for example, heat treatment such as dry heat treatment, steam treatment, superheated steam treatment, microwave treatment, hydrous ethanol treatment, high pressure treatment, and combinations thereof can be used.
 NSIが低すぎると、大豆乳化組成物中の蛋白質の割合が高くなりやすく、蛋白質に対する脂質含量が低くなる。また過加熱による焙煎臭等の雑味が生じやすくなる。逆にNSIが例えば80以上の高い数値になると大豆乳化組成物中の蛋白質の割合が低下し、大豆からの脂質の回収率も低下しやすくなる。また風味は青臭みが強くなる。
 例えば過熱水蒸気による加熱処理を行う場合、その処理条件は製造環境にも影響されるため一概に言えないが、おおよそ120~250℃の過熱水蒸気を用いて5~10分の間で加工大豆のNSIが上記範囲となるように処理条件を適宜選択すれば良く、加工処理に特段の困難は要しない。簡便には、NSIが上記範囲に加工された市販の大豆を用いることもできる。
When NSI is too low, the ratio of the protein in the soybean emulsified composition tends to be high, and the lipid content to the protein is low. Moreover, miscellaneous taste such as roasting odor due to overheating tends to occur. Conversely, when the NSI is a high value of 80 or more, for example, the protein ratio in the soybean emulsified composition decreases, and the lipid recovery rate from soybean tends to decrease. In addition, the flavor has a strong blue odor.
For example, when heat treatment with superheated steam is performed, the treatment conditions are influenced by the production environment, so it cannot be said unconditionally, but the NSI of processed soybeans is used for 5 to 10 minutes using superheated steam at approximately 120 to 250 ° C. The processing conditions may be selected as appropriate so that the value falls within the above range, and no particular difficulty is required for the processing. For convenience, commercially available soybeans with NSI processed in the above range can also be used.
 なお、NSIは所定の方法に基づき、全窒素量に占める水溶性窒素(粗蛋白)の比率(重量%)で表すことができ、本発明においては以下の方法に基づいて測定された値とする。
 すなわち、試料2.0gに100mlの水を加え、40℃にて60分攪拌抽出し、1400×gにて10分間遠心分離し、上清1を得る。残った沈殿に再度100mlの水を加え、40℃にて60分攪拌抽出し、1400×gにて10分遠心分離し、上清2を得る。上清1および上清2を合わせ、さらに水を加えて250mlとする。No.5Aろ紙にてろ過したのち、ろ液の窒素含量をケルダール法にて測定する。同時に試料中の窒素含量をケルダール法にて測定し、ろ液として回収された窒素(水溶性窒素)の試料中の全窒素に対する割合を重量%として表したものをNSIとする。
NSI can be expressed as a ratio (% by weight) of water-soluble nitrogen (crude protein) in the total amount of nitrogen based on a predetermined method. In the present invention, NSI is a value measured based on the following method. .
That is, 100 ml of water is added to 2.0 g of a sample, followed by stirring and extraction at 40 ° C. for 60 minutes, followed by centrifugation at 1400 × g for 10 minutes to obtain supernatant 1. 100 ml of water is added again to the remaining precipitate, followed by stirring and extraction at 40 ° C. for 60 minutes, and centrifugation at 1400 × g for 10 minutes to obtain supernatant 2. Supernatant 1 and supernatant 2 are combined, and water is further added to make 250 ml. After filtering with No. 5A filter paper, the nitrogen content of the filtrate is measured by Kjeldahl method. At the same time, the nitrogen content in the sample is measured by the Kjeldahl method, and the ratio of the nitrogen recovered as filtrate (water-soluble nitrogen) to the total nitrogen in the sample is expressed as weight%, which is NSI.
 前記の加工大豆は水抽出の前に、予め乾式又は湿式による粉砕、破砕、圧偏等の組織破壊処理を施されることが好ましい。組織破壊処理に際して、あらかじめ水浸漬や蒸煮により膨潤させても良く、これによって組織破壊に必要なエネルギーを低減させたり、ホエー蛋白質やオリゴ糖等の不快味を持つ成分を溶出させ除去できると共に、保水性やゲル化性の能力が高いグロブリン蛋白質(特にグリシニン及びβ-コングリシニン)の全蛋白質に対する抽出比率、すなわち水溶性画分への移行比率をより高めることができる。 It is preferable that the processed soybean is subjected to a tissue destruction treatment such as dry or wet pulverization, crushing, and pressure bias in advance before water extraction. In tissue destruction treatment, it may be swollen in advance by water immersion or steaming, thereby reducing the energy required for tissue destruction, and eluting and removing components with unpleasant taste such as whey protein and oligosaccharides, as well as water retention The extraction ratio of globulin proteins (especially glycinin and β-conglycinin) having a high ability and gelling ability to the total protein, that is, the transfer ratio to the water-soluble fraction can be further increased.
・原料大豆からの水抽出
 水抽出は含脂大豆に対して3~20重量倍、好ましくは4~15重量倍程度の加水をし、含脂大豆を懸濁させて行われる。加水倍率は高い方が水溶性成分の抽出率が高まり、分離を良くすることができるが、高すぎると濃縮が必要となりコストがかかる。また、抽出処理を2回以上繰り返すと水溶性成分の抽出率をより高めることができる。
-Water extraction from raw soybeans Water extraction is performed by adding 3 to 20 times, preferably 4 to 15 times, the amount of water added to fat-containing soybeans and suspending the fat-containing soybeans. The higher the rate of hydrolysis, the higher the extraction rate of the water-soluble component and the better the separation. However, if it is too high, concentration is required and costs increase. Further, when the extraction process is repeated twice or more, the extraction rate of the water-soluble component can be further increased.
 抽出温度には特に制限はないが、高い方が水溶性成分の抽出率が高まる反面、油脂も可溶化されやすくなり、大豆乳化組成物の脂質が低くなるため、70℃以下、好ましくは55℃以下で行うと良い。あるいは5~80℃、好ましくは50~75℃の範囲で行うこともできる。 The extraction temperature is not particularly limited. However, the higher the extraction rate of the water-soluble component, the more easily the oil and fat are solubilized, and the lower the lipid of the soybean emulsified composition. The following should be done. Alternatively, it can be carried out in the range of 5 to 80 ° C., preferably 50 to 75 ° C.
 抽出pH(加水後の大豆懸濁液のpH)も温度と同様に高いほうが水溶性成分の抽出率が高まる反面、油脂も可溶化されやすくなり、大豆乳化組成物の脂質が低くなる傾向にある。逆にpHが低すぎると蛋白質の抽出率が低くなる傾向にある。具体的には下限をpH6以上、もしくはpH6.3以上、もしくはpH6.5以上に調整して行うことができる。また上限は脂質の分離効率を上げる観点でpH9以下、もしくはpH8以下、もしくはpH7以下に調整して行うことができる。あるいは蛋白質の抽出率を高める観点でpH9~12のよりアルカリ性側に調整して行うことも可能である。 The higher the extraction pH (the pH of the soybean suspension after the addition of water), the higher the temperature, the higher the extraction rate of water-soluble components. . Conversely, if the pH is too low, the protein extraction rate tends to be low. Specifically, the lower limit can be adjusted to pH 6 or higher, pH 6.3 or higher, or pH 6.5 or higher. The upper limit may be adjusted to pH 9 or lower, pH 8 or lower, or pH 7 or lower from the viewpoint of increasing lipid separation efficiency. Alternatively, from the viewpoint of increasing the extraction rate of protein, it is possible to adjust the pH to 9 to 12 more alkaline.
・水抽出後の固液分離
 水抽出後、含脂大豆の懸濁液を遠心分離、濾過等により固液分離する。この際、中性脂質のみならず極性脂質も含めた大部分の脂質を水抽出物中に溶出させず、不溶化した蛋白質や食物繊維質の方に移行させ沈殿側(不溶性画分)とすることが重要である。具体的には含脂大豆の脂質の70重量%以上を沈殿側に移行させる。また抽出の際に上清側にも少量の脂質が溶出するが、豆乳中の脂質のように微細にエマルション化されたものではなく、15,000×g以下、あるいは5,000×g程度以下の遠心分離によっても容易に浮上させ分離することができ、この点で遠心分離機を使用するのが好ましい。なお遠心分離機は使用する設備によっては10万×g以上の超遠心分離を使用することも可能であるし、本発明に用いられる大豆乳化組成物の場合は超遠心分離機を用いなくとも実施が可能である。
 また水抽出の際あるいは水抽出後に解乳化剤を添加して豆乳からの脂質の分離を促進させることも可能であり、解乳化剤は特に限定されないが例えば特許文献2に開示されている解乳化剤を使用すればよい。ただし本発明に用いられる大豆乳化組成物を調製する場合は解乳化剤を用いなくとも実施が可能である。
-Solid-liquid separation after water extraction After water extraction, the suspension of fat-containing soybeans is subjected to solid-liquid separation by centrifugation, filtration or the like. At this time, not only neutral lipids but also most of the lipids including polar lipids are not eluted in the water extract, but transferred to the insolubilized protein or dietary fiber to make the precipitation side (insoluble fraction). is important. Specifically, 70% by weight or more of the lipid of the fat-containing soybean is transferred to the precipitation side. In addition, a small amount of lipid is also eluted on the supernatant side during extraction, but it is not finely emulsified like the lipid in soy milk, and it is centrifuged at 15,000 xg or less, or about 5,000 xg or less. In this respect, it is preferable to use a centrifuge. Depending on the equipment used, the centrifugal separator can be used for ultracentrifugation of 100,000 xg or more. In the case of the soybean emulsion composition used in the present invention, the centrifugal separator is not used. Is possible.
It is also possible to add a demulsifier during or after water extraction to promote lipid separation from soy milk. The demulsifier is not particularly limited, but for example, the demulsifier disclosed in Patent Document 2 is used. do it. However, the preparation of the soybean emulsified composition used in the present invention can be carried out without using a demulsifier.
 水抽出工程後の固液分離により、中性脂質のみならず極性脂質を不溶性画分に移行させ、これを回収することにより大豆乳化組成物の画分を得ることができる。
 固液分離として遠心分離を用いる場合、二層分離方式、三層分離方式のいずれも使用することができる。二層分離方式の場合は沈殿層である不溶性画分を回収する。また三層分離方式を用いる場合は、(1)浮上層(脂質を含む比重の最も小さいクリーム画分)、(2)中間層(脂質が少なく蛋白質、糖質を多く含む水溶性画分)、(3)沈殿層(脂質と食物繊維を多く含む不溶性画分)、の三層の画分に分けられる。この場合、脂質含量の少ない水溶性画分の中間層(2)を除去又は回収し、不溶性画分として浮上層(1)又は沈殿層(3)を回収するか、あるいは(1)と(3)を合わせて回収するとよい。
By solid-liquid separation after the water extraction step, not only neutral lipids but also polar lipids are transferred to an insoluble fraction, and a fraction of the soybean emulsified composition can be obtained by collecting this.
When centrifugation is used as the solid-liquid separation, either a two-layer separation method or a three-layer separation method can be used. In the case of the two-layer separation method, an insoluble fraction that is a precipitation layer is collected. When using the three-layer separation method, (1) floating layer (cream fraction with the smallest specific gravity containing lipid), (2) intermediate layer (water-soluble fraction containing a small amount of protein and carbohydrate), (3) It is divided into three-layer fractions: a sediment layer (insoluble fraction rich in lipids and dietary fibers). In this case, the intermediate layer (2) of the water-soluble fraction having a low lipid content is removed or recovered, and the floating layer (1) or the precipitated layer (3) is recovered as an insoluble fraction, or (1) and (3 ).
 得られた不溶性画分(1)、(3)はそのまま、あるいは必要により濃縮工程、加熱殺菌工程、粉末化工程等を経て本発明に用いられる大豆乳化組成物とすることができる。 The obtained insoluble fractions (1) and (3) can be used as they are, or, if necessary, through a concentration step, a heat sterilization step, a powdering step, etc. to obtain a soybean emulsion composition used in the present invention.
・食物繊維の除去
 得られた不溶性画分が食物繊維を含む場合、例えば上記(3)又は(1)及び(3)の画分である場合、必要により加水し、高圧ホモゲナイザーあるいはジェットクッカー加熱機等による均質化した後、該均質化液をさらに固液分離して上清を回収する工程を経ることにより、食物繊維(オカラ)を除去することもでき、コクのある風味がより濃縮された大豆乳化組成物を得ることができる。該均質化の前後いずれかにおいて必要により加熱処理工程、アルカリ処理工程等を付加することにより蛋白質をより抽出しやすくすることもできる。この場合、乾物あたりの食物繊維含量は10重量%以下であり、5重量%以下がより好ましい。なお、本発明において食物繊維含量は、「五訂増補日本食品標準成分表」(文部科学省、2005)に準じ、酵素-重量法(プロスキー変法)により測定することができる。
・ Removal of dietary fiber When the obtained insoluble fraction contains dietary fiber, for example, when it is the fraction of (3) or (1) and (3) above, water is added if necessary, and a high-pressure homogenizer or jet cooker heater After homogenization by means of, etc., through the process of further solid-liquid separation of the homogenized liquid and collecting the supernatant, dietary fiber (ocara) can also be removed, and the rich flavor is further concentrated A soybean emulsified composition can be obtained. Proteins can be more easily extracted by adding a heat treatment step, an alkali treatment step or the like, if necessary, before or after the homogenization. In this case, the dietary fiber content per dry matter is 10% by weight or less, and more preferably 5% by weight or less. In the present invention, the dietary fiber content can be measured by the enzyme-weight method (modified Prosky method) according to the “Fifth Supplement Japanese Food Standard Ingredient Table” (Ministry of Education, Culture, Sports, Science and Technology, 2005).
(大豆乳化組成物の特徴)
 本発明に用いられる上記の大豆乳化組成物は、脂質(中性脂質及び極性脂質)及び蛋白質が特定の範囲で含まれ、蛋白質のうち特にLP含量が高く、必要により繊維質も含まれる乳化組成物であり、大豆が本来有する自然な美味しさが濃縮されており、従来の問題とされていた青臭味や収斂味、渋味等の不快味がないか非常に少なく、非常にコクのある風味を有するものである。
 通常の大豆粉や分離大豆蛋白に水、油脂を加えて該大豆乳化組成物と類似の組成の乳化組成物にすることは可能であるが、リポキシゲナーゼ蛋白質含量あるいはLCI値を同等なレベルに調整することは困難である。そして本技術により調製された大豆乳化組成物は、このような組み立て製品に比べて格段に風味が良好であり、食品素材としての利用適性が高いことに特徴を有する。
(Characteristics of soybean emulsified composition)
The above-mentioned soybean emulsified composition used in the present invention contains a lipid (neutral lipid and polar lipid) and protein in a specific range, has a particularly high LP content of protein, and optionally contains fiber. The natural taste of soy is concentrated, and there is little or no unpleasant taste such as blue odor, astringent taste, astringency, etc. It has a flavor.
It is possible to add water and fat to normal soy flour and isolated soy protein to make an emulsified composition similar to the soy emulsified composition, but adjust the lipoxygenase protein content or LCI value to an equivalent level It is difficult. And the soybean emulsified composition prepared by this technique is characterized by remarkably good flavor and high suitability for use as a food material compared to such an assembled product.
(高温加熱処理)
 本発明は、少なくとも上記大豆乳化組成物に対して、高温加熱処理を施すことが重要である。かかる加熱処理を特定の条件で行うことにより大豆乳化組成物のたん白質が変性しチーズ様の滑らかな食感を有する豆腐になることが特徴である。
 高温加熱処理は、例えばプレート式熱交換機、チューブ式熱交換機、掻き取り式熱交換機、スチームインジェクション式熱交換機等の各種熱交換機やバッチ式のレトルト釜等、従来公知の機器を使用できる。これらの機器は、任意に組み合わせて使用してもよい。
(High temperature heat treatment)
In the present invention, it is important to perform a high-temperature heat treatment on at least the soybean emulsion composition. By performing such heat treatment under specific conditions, the protein of the soybean emulsified composition is denatured and becomes a tofu having a cheese-like smooth texture.
For the high-temperature heat treatment, conventionally known devices such as various heat exchangers such as a plate heat exchanger, a tube heat exchanger, a scraping heat exchanger, and a steam injection heat exchanger, and a batch retort kettle can be used. These devices may be used in any combination.
(加熱処理条件)
 本発明の目的を達成するためには、加熱処理条件が重要であり、単に殺菌を目的とする程度の加熱条件では困難である。
 すなわち、加熱温度は少なくとも135℃以上で行うことが重要であり、より好ましくは138~145℃、さらに好ましくは138~142℃である。加熱温度が低いと、豆腐の食感がチーズ様の滑らかな食感を有さない場合がある。さらに、加熱時間は少なくとも2秒超で行うことが重要であり、好ましくは2~40秒、より好ましくは4~20秒が適当である。
 加熱時間が短い場合、得られた豆腐の食感が、チーズ様の滑らかな食感を有さない場合がある。
(Heat treatment conditions)
In order to achieve the object of the present invention, the heat treatment conditions are important, and it is difficult to perform the heat conditions only for the purpose of sterilization.
That is, it is important that the heating temperature is at least 135 ° C. or more, more preferably 138 to 145 ° C., and further preferably 138 to 142 ° C. When the heating temperature is low, the texture of tofu may not have a cheese-like smooth texture. Furthermore, it is important that the heating time be at least 2 seconds, preferably 2 to 40 seconds, more preferably 4 to 20 seconds.
When the heating time is short, the texture of the obtained tofu may not have a cheese-like smooth texture.
 大豆乳化組成物に高温加熱処理を施した後、必ずしも必須ではないが、加熱処理された大豆乳化組成物をホモゲナイザー等により均質化することができる。例えば高圧ホモゲナイザーを用いる場合は圧力3~15MPaが適当である。 After the high-temperature heat treatment is performed on the soybean emulsion composition, the heat-treated soybean emulsion composition can be homogenized with a homogenizer or the like, although not necessarily essential. For example, when a high-pressure homogenizer is used, a pressure of 3 to 15 MPa is appropriate.
(チーズ様豆腐類)
  本発明に使用する大豆乳化組成物、あるいは大豆乳化組成物と豆乳との混合物に凝固剤を加え、加熱することにより蛋白質を凝固させ、チーズ様の滑らかな食感 を有するチーズ様豆腐を製造することができる。チーズ様の滑らかな食感とは、軟質チーズ類が有する独特の滑らかな食感を言う。この軟質チーズ類とは、具体的には、クリームチーズやマスカルポーネチーズ、クワルクチーズ、フロマージュブラン等があげられるが、これに限定するものではない。
(Cheese-like tofu)
A coagulant is added to the soybean emulsified composition used in the present invention or a mixture of the soy emulsified composition and soy milk, and the protein is coagulated by heating to produce a cheese-like tofu having a cheese-like smooth texture. be able to. Cheese-like smooth texture refers to the unique smooth texture of soft cheeses. Specific examples of the soft cheeses include cream cheese, mascarpone cheese, quark cheese, and fromage blanc, but are not limited thereto.
 本発明に使用する大豆乳化組成物、あるいは大豆乳化組成物と豆乳との混合物に凝固剤を加え、加熱することにより蛋白質を凝固させ、チーズ様の滑らかな食感を有するチーズ様豆腐を製造することができる。チーズ様豆腐の製造は公知の製法で行うことができ、特に困難なく製造が可能である。凝固剤としてはニガリ(塩化マグネシウム)、塩化カルシウム、硫酸カルシウム、GDLなどを使用することができる。凝固剤の添加時期は、大豆乳化組成物に対して加熱温度135℃以上、加熱時間2秒以上の高温加熱処理を施した後が好ましい。大豆乳化組成物に対して、高温加熱処理が行われない場合、チーズ様の滑らかな食感が得られない場合がある。
 凝固剤の添加量は特に限定されないが、例えば大豆乳化組成物及び豆乳の乾物に対して0.1~5重量%、好ましくは0.3~3重量%とすることができる。よりチーズ様の滑らかな食感をだす場合、大豆乳化組成物のみを原料とし、凝固剤の量を少なめに添加すればよい。概して得られたチーズ様豆腐は、軟質チーズ類に類似した、非常にクリーミーで口の中でとろけるような口溶けの良い食感を有しており、従来の滑らかな豆腐では得られない食感を有するものとなっている。
  なお、チーズ様豆腐には一般の製造において添加されている種々の添加剤を添加することができる。例えば凝固剤以外に塩類、オリゴ糖、多糖類、澱粉類、タンパク質などを単独あるいは併用して添加することができる。
A coagulant is added to the soybean emulsified composition used in the present invention or a mixture of the soybean emulsified composition and soy milk, and the protein is coagulated by heating to produce a cheese-like tofu having a cheese-like smooth texture. be able to. Cheese-like tofu can be produced by a known production method and can be produced without any particular difficulty. As the coagulant, bittern (magnesium chloride), calcium chloride, calcium sulfate, GDL and the like can be used. The coagulant is preferably added after the soybean emulsion composition is subjected to a high-temperature heat treatment at a heating temperature of 135 ° C. or more and a heating time of 2 seconds or more. When the high-temperature heat treatment is not performed on the soybean emulsion composition, a cheese-like smooth texture may not be obtained.
The amount of the coagulant added is not particularly limited, but may be, for example, 0.1 to 5% by weight, preferably 0.3 to 3% by weight, based on the soybean emulsified composition and the dried soybean milk. In order to produce a cheese-like smooth texture, only the soybean emulsified composition may be used as a raw material, and a small amount of the coagulant may be added. In general, the cheese-like tofu obtained has a texture that is very creamy and melts in the mouth, similar to soft cheeses, and has a texture that cannot be obtained with conventional smooth tofu. It has become.
In addition, the various additives currently added in general manufacture can be added to cheese-like tofu. For example, in addition to the coagulant, salts, oligosaccharides, polysaccharides, starches, proteins and the like can be added alone or in combination.
 また、上記大豆乳化組成物を用いた豆腐を厚揚げの原材料に使用することにより、チーズ様の滑らかな食感を有した厚揚げもできる。 Moreover, by using tofu using the above-mentioned soybean emulsified composition as a raw material for deep-fried food, deep-fried food with a smooth texture like cheese can be obtained.
 以下に実施例を記載するが、この発明の技術思想がこれらの例示によって限定されるものではない。なお、以下特に断りのない限り、「%」と「部」は重量基準である。 Examples are described below, but the technical idea of the present invention is not limited to these exemplifications. Unless otherwise specified, “%” and “parts” are based on weight.
(製造例1) 大豆乳化組成物の調製
 特開2012-016348号の実施例1の記載に基づいて、大豆乳化組成物を調製した。得られた大豆乳化組成物の乾物含量は30.6%、乾物あたり蛋白量および脂質量はそれぞれ32.2%、43.0%であり、LCI値は67%であった。
(Production Example 1) Preparation of soybean emulsified composition A soybean emulsified composition was prepared based on the description in Example 1 of JP2012-016348A. The resulting soybean emulsified composition had a dry matter content of 30.6%, a protein amount and a lipid amount per dry matter of 32.2% and 43.0%, respectively, and an LCI value of 67%.
(試作実験例1) 
 製造例1で得られた大豆乳化組成物に対して、プレート式UHT殺菌装置(パワーポイント・インターナショナル社製)にて加熱時間4秒に固定し、加熱温度を120℃、130℃、135℃、140℃、145℃、150℃にて高温加熱処理した。加熱処理した大豆乳化組成物100部に対して、塩田にがり小袋(赤穂化成(株))3.4部を添加し、蒸し器にて7分30秒蒸し、氷冷した。
 作製した豆腐に対し官能評価を行い、5段階にて評価した。食感は、1を豆腐のように硬い食感、5をチーズのように滑らかな食感として評価した(4.0以上を合格)。また、風味は、1をコクがなくスッキリとした風味、5をコクがあり濃厚な風味として評価した(4.0以上を合格)。結果を、表2に示した。
(Prototype experiment example 1)
The soybean emulsified composition obtained in Production Example 1 is fixed to a heating time of 4 seconds with a plate-type UHT sterilizer (manufactured by Powerpoint International Co., Ltd.), and the heating temperature is 120 ° C., 130 ° C., 135 ° C., 140 High-temperature heat treatment was performed at 145 ° C. and 150 ° C. To 100 parts of the heat-treated soybean emulsified composition, 3.4 parts of Shiota nigari sachet (Ako Kasei Co., Ltd.) was added, steamed in a steamer for 7 minutes 30 seconds, and cooled with ice.
Sensory evaluation was performed on the produced tofu and evaluated in five stages. The texture was evaluated as 1 for a hard texture like tofu and 5 for a smooth texture like cheese (passed 4.0 or higher). In addition, the flavor was evaluated as 1 with a rich and refreshing flavor, and 5 with a rich and rich flavor (4.0 or higher passed). The results are shown in Table 2.
(表2)結果 加熱温度の効果
Figure JPOXMLDOC01-appb-I000003
(Table 2) Results Effect of heating temperature
Figure JPOXMLDOC01-appb-I000003
 実施例1から4に示す通り、加熱温度135℃以上において、チーズ様のクリーミーな食感を有する豆腐となった。 As shown in Examples 1 to 4, the tofu had a cheese-like creamy texture at a heating temperature of 135 ° C. or higher.
(試作実験例2)
 製造例1で得られた大豆乳化組成物に対して、プレート式UHT殺菌装置(パワーポイント・インターナショナル社製)にて加熱温度140℃に固定し、加熱温度を2秒、4秒、20秒、40秒、60秒にて高温加熱処理した。加熱処理した大豆乳化組成物100部に対して、塩田にがり小袋(赤穂化成(株))3.4部を添加し、蒸し器にて7分30秒蒸し、氷冷した。
作製した豆腐に対し官能評価を行い、5段階にて評価した。食感は、1を豆腐のように硬い食感、5をチーズのように滑らかな食感として評価した(4.0以上を合格)。また、風味は、1をコクがなくスッキリとした風味、5をコクがあり濃厚な風味として評価した(4.0以上を合格)。結果を、表3に示した。
(Prototype experiment example 2)
The soybean emulsified composition obtained in Production Example 1 is fixed at a heating temperature of 140 ° C. with a plate-type UHT sterilizer (manufactured by Powerpoint International), and the heating temperature is 2 seconds, 4 seconds, 20 seconds, 40 High-temperature heat treatment was performed in seconds and 60 seconds. To 100 parts of the heat-treated soybean emulsified composition, 3.4 parts of Shiota nigari sachet (Ako Kasei Co., Ltd.) was added, steamed in a steamer for 7 minutes 30 seconds, and cooled with ice.
Sensory evaluation was performed on the produced tofu and evaluated in five stages. The texture was evaluated as 1 for a hard texture like tofu and 5 for a smooth texture like cheese (passed 4.0 or higher). In addition, the flavor was evaluated as 1 with a rich and refreshing flavor, and 5 with a rich and rich flavor (4.0 or higher passed). The results are shown in Table 3.
(表3)結果 加熱時間の効果
Figure JPOXMLDOC01-appb-I000004
(Table 3) Results Effect of heating time
Figure JPOXMLDOC01-appb-I000004
 実施例5~9に示す通り、加熱時間2秒以上において、チーズ様のクリーミーな食感を有する豆腐となった。特に4秒から20秒では、非常に滑らかなチーズ様食感が得られた。(実施例6、実施例7) As shown in Examples 5 to 9, tofu having a cheese-like creamy texture was obtained at a heating time of 2 seconds or more. Especially in 4 to 20 seconds, a very smooth cheese-like texture was obtained. (Example 6, Example 7)
(試作実験例3)
 製造例1で得られた大豆乳化組成物に対して、プレート式UHT殺菌装置(パワーポイント・インターナショナル社製)にて加熱温度130℃に固定し、加熱温度を2秒、4秒、20秒、40秒、60秒にて高温加熱処理した。加熱処理した大豆乳化組成物100部に対して、塩田にがり小袋(赤穂化成(株))3.4部を添加し、蒸し器にて7分30秒蒸し、氷冷した。
 作製した豆腐に対し官能評価を行い、5段階にて評価した。食感は、1を豆腐のように硬い食感、5をチーズのように滑らかな食感として評価した(4.0以上を合格)。また、風味は、1をコクがなくスッキリとした風味、5をコクがあり濃厚な風味として評価した(4.0以上を合格)。結果を、表4に示した。
(Prototype experimental example 3)
The soybean emulsified composition obtained in Production Example 1 is fixed at a heating temperature of 130 ° C. with a plate-type UHT sterilizer (manufactured by Powerpoint International), and the heating temperature is 2 seconds, 4 seconds, 20 seconds, 40 High-temperature heat treatment was performed in seconds and 60 seconds. To 100 parts of the heat-treated soybean emulsified composition, 3.4 parts of Shiota nigari sachet (Ako Kasei Co., Ltd.) was added, steamed in a steamer for 7 minutes 30 seconds, and cooled with ice.
Sensory evaluation was performed on the produced tofu and evaluated in five stages. The texture was evaluated as 1 for a hard texture like tofu and 5 for a smooth texture like cheese (passed 4.0 or higher). In addition, the flavor was evaluated as 1 with a rich and refreshing flavor, and 5 with a rich and rich flavor (4.0 or higher passed). The results are shown in Table 4.
(表4)結果 加熱時間の効果
Figure JPOXMLDOC01-appb-I000005
(Table 4) Results Effect of heating time
Figure JPOXMLDOC01-appb-I000005
 いずれの加熱時間においても、チーズ様のクリーミーな食感を有する豆腐は得られなかった。(比較例3~比較例7) No tofu with a creamy texture like cheese was obtained at any heating time. (Comparative Examples 3 to 7)
 本発明では、脂質親和性蛋白質が一般の大豆素材より多く含まれることが特徴である大豆乳化組成物に対して、特定条件の高温加熱処理を施すことで、従来の豆腐食感とは異なる、チーズ様な滑らかな食感のチーズ様豆腐を得ることが可能となる。このチーズ様豆腐は、豆腐の食シーンの多様化という消費者ニーズにマッチしており、豆腐市場の活性化に大いに寄与することが期待される。 In the present invention, the soybean emulsified composition characterized in that the lipophilic protein is contained in a larger amount than a general soybean material, by performing a high-temperature heat treatment under specific conditions, it is different from the conventional bean corrosion feeling. It becomes possible to obtain a cheese-like tofu having a smooth texture like cheese. This cheese-like tofu matches consumer needs for diversifying the tofu food scene and is expected to greatly contribute to the activation of the tofu market.

Claims (4)

  1.  乾物あたりの蛋白質含量が25重量%以上、脂質含量(クロロホルム/メタノール混合溶媒抽出物としての含量をいう。)が蛋白質含量に対して100重量%以上である大豆乳化組成物に対して、加熱温度135℃以上、加熱時間2秒以上の高温加熱処理を施した後凝固剤を加えることを特徴とするチーズ様豆腐の製造方法。 Heating temperature for soybean emulsified composition having a protein content of 25% by weight or more per dry matter and a lipid content (referred to as a chloroform / methanol mixed solvent extract) of 100% by weight or more based on the protein content A method for producing cheese-like tofu, characterized by adding a coagulant after high-temperature heat treatment at 135 ° C or higher and a heating time of 2 seconds or longer.
  2.  大豆乳化組成物が豆乳であること特徴とする、請求項1記載のチーズ様豆腐の製造方法。 The method for producing cheese-like tofu according to claim 1, wherein the soybean emulsified composition is soy milk.
  3.  加熱温度が135~145℃であることを特徴とする、請求項1記載のチーズ様豆腐の製造方法。 The method for producing cheese-like tofu according to claim 1, wherein the heating temperature is 135 to 145 ° C.
  4.  加熱時間が4~20秒であることを特徴とする、請求項1記載のチーズ様豆腐の製造方法。 2. The method for producing cheese-like tofu according to claim 1, wherein the heating time is 4 to 20 seconds.
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WO2011071108A1 (en) * 2009-12-10 2011-06-16 朝日食品工業株式会社 Soy milk containing fibrous material and tofu-like food, processes for production of those products, and dehulled soybean powdery material
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WO2007116772A1 (en) * 2006-03-28 2007-10-18 Fuji Oil Company, Limited Process for producing powdery soy milk and application of the same
WO2011071108A1 (en) * 2009-12-10 2011-06-16 朝日食品工業株式会社 Soy milk containing fibrous material and tofu-like food, processes for production of those products, and dehulled soybean powdery material
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Publication number Priority date Publication date Assignee Title
WO2019188788A1 (en) * 2018-03-29 2019-10-03 不二製油グループ本社株式会社 Method for manufacturing soybean paste food or gel food

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