WO2012134241A3 - Ｃｍａｈ 유전자를 표적으로 하는 징크 핑거 뉴클레아제 및 이의 용도 - Google Patents

Ｃｍａｈ 유전자를 표적으로 하는 징크 핑거 뉴클레아제 및 이의 용도 Download PDF

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Publication number
WO2012134241A3
WO2012134241A3 PCT/KR2012/002419 KR2012002419W WO2012134241A3 WO 2012134241 A3 WO2012134241 A3 WO 2012134241A3 KR 2012002419 W KR2012002419 W KR 2012002419W WO 2012134241 A3 WO2012134241 A3 WO 2012134241A3
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WIPO (PCT)
Prior art keywords
cmah
zinc finger
finger nuclease
gene
knocked out
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PCT/KR2012/002419
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English (en)
French (fr)
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WO2012134241A2 (ko
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김진수
김석중
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주식회사 툴젠
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Publication of WO2012134241A2 publication Critical patent/WO2012134241A2/ko
Publication of WO2012134241A3 publication Critical patent/WO2012134241A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A01K2267/025—Animal producing cells or organs for transplantation

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 돼지 CMAH(swine cytidine monophospho-N-acetylneuraminic acid hydroxylase) 유전자가 녹아웃된 돼지 세포의 제조방법; 돼지 CMAH 유전자가 녹아웃된 돼지의 제조 방법; 돼지 CMAH 유전자가 녹아웃된 돼지 유래 인공장기 또는 인공조직의 제조 방법; CMAH 유전자를 표적으로 하는 징크 핑거 뉴클레아제; 상기 징크 핑거 뉴클레아제를 코딩하는 폴리뉴클레오티드; 상기 폴리뉴클레오티드를 포함하는 벡터; 상기 징크 핑거 뉴클레아제를 사용하여 CMAH 유전자가 녹아웃된 세포를 제조하는 방법; 및 CMAH 유전자가 녹아웃된 인간 이외 동물의 난자, 인간 이외 동물, 또는 인간 이외 동물 유래 인공장기 제조용 키트에 관한 것이다. 본 발명에 따르면, CMAH 유전자에 대한 징크 핑거 뉴클레아제를 사용하여, CMAH 유전자가 제거된 이종장기이식용 돼지생산을 위한 형질전환세포를 대량 확보할 수 있다.
PCT/KR2012/002419 2011-03-30 2012-03-30 Ｃｍａｈ 유전자를 표적으로 하는 징크 핑거 뉴클레아제 및 이의 용도 WO2012134241A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161469171P 2011-03-30 2011-03-30
US61/469,171 2011-03-30

Publications (2)

Publication Number Publication Date
WO2012134241A2 WO2012134241A2 (ko) 2012-10-04
WO2012134241A3 true WO2012134241A3 (ko) 2013-01-10

Family

ID=46932183

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2012/002419 WO2012134241A2 (ko) 2011-03-30 2012-03-30 Ｃｍａｈ 유전자를 표적으로 하는 징크 핑거 뉴클레아제 및 이의 용도

Country Status (2)

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KR (1) KR101896518B1 (ko)
WO (1) WO2012134241A2 (ko)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090056922A (ko) * 2007-11-30 2009-06-03 한국생명공학연구원 이종 장기 이식용 미니 복제돼지 생산을 위한 유전자 조작된 세포주 및 그의 생산방법
US20090176653A1 (en) * 2001-08-17 2009-07-09 Toolgen, Inc. Zinc finger domain libraries

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2702148C (en) 1999-01-06 2014-03-04 Genenews Inc. Method of profiling gene expression in a human subject having an infectious disease

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090176653A1 (en) * 2001-08-17 2009-07-09 Toolgen, Inc. Zinc finger domain libraries
KR20090056922A (ko) * 2007-11-30 2009-06-03 한국생명공학연구원 이종 장기 이식용 미니 복제돼지 생산을 위한 유전자 조작된 세포주 및 그의 생산방법

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MEYER, M. ET AL.: "Gene targeting by homologous recombination in mouse zygotes mediated by zinc-fmger nucleases", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 107, no. 34, 4 August 2010 (2010-08-04), pages 15022 - 15026 *
SONG, K. H. ET AL.: "Cloning and functional characterization of pig CMP-N-acetylneuraminic acid hydroxylase for the synthesis ofN-glycolyneuraminic acid as the xenoantigenic determinant in pig-human xenotransplantation", BIOCHEMICAL JOURNAL, vol. 427, no. 1, 15 March 2010 (2010-03-15), pages 179 - 188 *
WATANABE, M. ET AL.: "Knockout of exogenous EGFP gene in porcine somatic cells using zinc-finger nucleases", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS., vol. 402, no. 1, 26 September 2010 (2010-09-26), pages 14 - 18, XP002659662, DOI: doi:10.1016/J.BBRC.2010.09.092 *

Also Published As

Publication number Publication date
WO2012134241A2 (ko) 2012-10-04
KR20120112266A (ko) 2012-10-11
KR101896518B1 (ko) 2018-10-24

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