WO2012073226A2 - Process to produce cultured pearl product through transplantation by using natural pearl nucleus waste - Google Patents
Process to produce cultured pearl product through transplantation by using natural pearl nucleus waste Download PDFInfo
- Publication number
- WO2012073226A2 WO2012073226A2 PCT/ID2011/000006 ID2011000006W WO2012073226A2 WO 2012073226 A2 WO2012073226 A2 WO 2012073226A2 ID 2011000006 W ID2011000006 W ID 2011000006W WO 2012073226 A2 WO2012073226 A2 WO 2012073226A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- pearl
- nucleus
- natural
- oyster
- waste
- Prior art date
Links
- 239000011050 natural pearl Substances 0.000 title claims abstract description 65
- 239000002699 waste material Substances 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims abstract description 46
- 230000008569 process Effects 0.000 title claims abstract description 42
- 238000002054 transplantation Methods 0.000 title claims abstract description 34
- 239000010984 cultured pearl Substances 0.000 title claims abstract description 26
- 239000011049 pearl Substances 0.000 claims abstract description 109
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 24
- 210000000981 epithelium Anatomy 0.000 claims abstract description 21
- 230000002710 gonadal effect Effects 0.000 claims abstract description 20
- 229910052709 silver Inorganic materials 0.000 claims abstract description 13
- 239000004332 silver Substances 0.000 claims abstract description 13
- 239000011780 sodium chloride Substances 0.000 claims abstract description 12
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229910052681 coesite Inorganic materials 0.000 claims abstract description 8
- 229910052906 cristobalite Inorganic materials 0.000 claims abstract description 8
- 229910052682 stishovite Inorganic materials 0.000 claims abstract description 8
- 229910052905 tridymite Inorganic materials 0.000 claims abstract description 8
- 241000237502 Ostreidae Species 0.000 claims description 33
- 241000490567 Pinctada Species 0.000 claims description 33
- 235000020636 oyster Nutrition 0.000 claims description 33
- 210000001519 tissue Anatomy 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 230000036541 health Effects 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 239000003242 anti bacterial agent Substances 0.000 claims description 5
- 229940088710 antibiotic agent Drugs 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 4
- 239000000243 solution Substances 0.000 abstract description 11
- 238000002347 injection Methods 0.000 abstract description 9
- 239000007924 injection Substances 0.000 abstract description 9
- 229910017344 Fe2 O3 Inorganic materials 0.000 abstract 1
- 239000000377 silicon dioxide Substances 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 description 11
- 229910018404 Al2 O3 Inorganic materials 0.000 description 3
- 210000002149 gonad Anatomy 0.000 description 3
- 230000001788 irregular Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003653 coastal water Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000012487 rinsing solution Substances 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Definitions
- This invention is generally relating to a process to produce cultured pearl product through transplantation by using natural pearl waste, more specifically the process to produce cultured pearl product through transplantation by using natural pearl waste with injection of softened epithelium cell to become a small cut which is injected with 0.90% sodium chloride solution into gonadal cavity to touch the surface of natural pearl nucleus as its transplant.
- Transplantation function as we know that by implanting grains or nuclei existing inside pearl body within long period of time, then such nuclei are coated by resin produced by pearl body and the longer the time the more harden and coat the whole nuclei so that it is obtained cultured pearl within relatively short period from 1 .5 years to 2 years to obtain pearl product by harvesting such pearls.
- the first factor inventor considers that I Indonesia has supporting natural geography to obtain pearl that has been explores within long period of time, in which in searching natural pearl there were so many damaged pearl oyster, that eventually it become scarce year by year, then in such search there is always any pearl waste left that so far will stack to become natural pearl waste material, in which it has non standard shape with smaller size of pearl feasible to become pearl ornamental material, those all become waste since such materials are unsold to trade.
- the second factor inventor tries to make process to produce pearl culture by nucleus transplant made of natural pearl waste so that it boosts benefit of unmarketable natural pearl waste to marketable pearl object through pearl culture with special process such as treating pearl oyster to obtain pearl grain waste to become pearl close to original pearl.
- the third factor, inventor provides a process to produce pearl culture through transplantation with natural pearl waste nucleus by providing special equipments and treatment with different process from pearl culture as existing previously.
- the fourth factor, inventor provides a process to make pearl culture product through nucleus transplantation by using natural pearl waste since pearl waste that has been stacked for years in Indonesia as a result of exploration of unusable waste into valuable and value added pearl product, a process of making pearl culture from the nucleus transplant of pearl waste.
- the fifth factor is to make a pearl getting close to natural pearl by adding values from valueless pearl waste to high valuable pearl.
- the sixth factor is to make use of pearl waste owned by Indonesia in order to be re-utilized into feasibly-sold pearl by culturing pearl that uses nucleus transplanted into oyster pearl to be made cultured pearl.
- the seventh factor is to use existing pearl waste by applying process to implement nucleus in use as a grain implanted into cultured oyster pearl that eventually produces pearl from natural pearl waste to be made high-quality pearl or equivalent to natural pearl quality. Based on above existing factor, then inventor tries to make a process to produce pearl by using natural pearl waste as a nucleus in order to make good and high-quality natural pearl culture by considering the following aspects:
- the first aspect inventor provides a process to make pearl by using natural pearl as nucleus to be transplanted into oyster pearl as pearl culture oyster to produce high quality pearl.
- the second aspect inventor provides a process to make pearl product by using natural pearl waste with the following steps:
- the seventh step to slice leg's part until gonadal tissues (oyster reproduction area);
- the eight step to enter pearl nucleus through cavity of slicing on gonadal layer;
- Epithelium cell in the form of cell of small slice mixed with Sodium Chloride solution 0.90% which is injected until it touches outer wall of nucleus on gonadal position on pearl oyster body.
- the fourth aspect inventor provides an equipment to produce pearl by using natural pearl waste with the following equipment composition
- epithelium cell is epithelium cell taken out from blended or destroyed oyster body to become refined form to be mixed with sodium chloride solution 0.90% to obtain diluted solution thus it can be injected into body cultured pearl tissues easily through slicing hole in pearl body to reach gonad (or oyster reproduction area) with size ratio of 50: 50 in order to be injected to gonadal cavity on sliced oyster body to touch outer skin surface of such nucleus, thus nucleus in body is eventually coated by body fluid from enzyme produced by oyster body to change into pearl.
- Term of "injection” is an injection facility to distribute fluid in the form of oyster epithelium cell which can accelerate the formation of pearl layer in oyster body by way of injection.
- natural oyster waste is semi-finished pearl grains produced by valueless natural pearl oyster that becoming waste with smaller diameter than standard of pearl as jewelry. And various forms of grain and color, from white to black, waste pearl produced from pearl exploitation is obtained unusable grains as pearl material for jewelry.
- nucleus is material to produce a pearl by implanting it in oyster body through slicing and put nucleus into gonad tissues layer.
- the objective of this invention is to provide process to produce pearl by using natural pearl waste as nucleus to be transplanted into pearl oyster as pearl culture oyster to produce high quality pearl.
- Another objective of this invention is to provide a process to produce a pearl by using nucleus and valueless natural pearl waste to become valuable one.
- Other objective of this invention is to provide a process to produce pearl product by using natural pearl waste with the following steps;
- Epithelium cell in the form of small cuts mixed with Sodium chloride solution 0.90% which is injected until it touches outer wall of nucleus on gonadal position in pearl oyster body.
- Equipment to produce pearl by using natural pearl waste as nucleus which is implanted into cultured pearl oyster by using epithelium cel l injection process by applying injection equipment having predetermined epithelium cell dosage in order that epithelium cell can touch outer layer surface of pearl oyster waste nucleus in body of pearl culture oyster.
- Picture l a demonstrates a picture of U-5 from natural bred pearl grain and picture l b is a picture of transplanted pearl product after being harvested with the process to produce cultured pearl product by transplantation by using natural pearl waste.
- Picture 2a demonstrates the picture of U-6 from cultured natural pearl grain and Picture 2b is a picture of transplanted pearl product after being harvested with the process to produce cultured pearl product through transplantation by using natural pearl waste.
- Picture 3a demonstrates the picture of U-7 of cultured natural pearl grain and Picture 3b is a picture of transplanted pearl product after being harvest with the process to produce cultured pearl through transplantation by using natural pearl waste.
- Picture 4a demonstrates the picture of U-8 of cultured natural pearl grain and Picture 4b is a picture of transplanted pearl product after being harvested with the process to produce cultured pearl product through transplantation by using natural pearl waste.
- Picture 5 a demonstrates the picture of U-9 of cultured natural pearl grain and Picture %b is a picture of transplanted pearl product after being harvested with the process to produce cultured pearl through transplantation by using natural pearl waste.
- nucleus is washed with alcohol 70% to 95%, in order that nucleus made of natural pearl waste is clean and free from contamination.
- Insertive process is to prepare nucleus into cultured oyster body that has been prepared in step on procedures to prepare nucleus for nucleus insertive process.
- the second step is to slice leg' s part until it reaches gonadal layer (oyster reproduction area).
- Transplantation is marked by predetermined date, namely 16-06-2009 and harvest day of 16-06-2010 with the silver pearl product measuring 0.5mm to 10mm, such dimension can be found as showed in Picture2b silver and white pearl product in various sizes of 0.25mm to 10 mm which have the contents of COC0 3 of 80.82% weight Mg C0 3 of 2.16% weight, Ca 3 (P0 4 ) 2 of 0.16% weight, Si0 2 of 9.54% weight, the rest are Al 2 O3 + Fe 2 03 with the temperature under H OC 0 with evaporated H 2 0 of 13.44% weight and decrease around 30-40% from the total pearl oyster implanted after harvest they have varying sizes with the process to produce cultured pearl product through transplantation by using natural pearl waste in accordance with this invention.
- Sample is taken from U-7 through the process to produce cultured pearl product through transplantation by using natural pearl waste in the form of irregular elliptical through pre-registered transplantation process with total 612 pearl oysters (Picture 3 a), each pearl oyster is transplanted and each transplantation marked by predetermined date, namely 22-06-2009 and on harvest date of 30-09-2010 with silver pearl product measuring 0.5mm to 10mm can be found as showed in picture 3b, silver and white pearl product in varying sizes from 0.25mm to 10mm having the contents of COC0 3 of 80.82% weight, Mg C0 3 of 2.16% weight, Ca 3 (P0 4 ) 2 of 0.16% weight, Si0 2 of 9.54% weight, the rest are Al 2 O3 + Fe 2 03 with the temperature under 1 10C° with evaporated H 2 0 of 13.44% weight and decrease around 30-40% of total pearl oyster implanted and after harvest they have varying sizes with the process to produce cultured pearl product through transplantation by using natural pearl waste in accordance with this invention.
- Sample is taken from U-8 through the process to produce cultured pearl product through transplantation by using natural pearl waste in the form of irregular elliptical through pre-registered transplantation process with total 205 pearl oysters (picture 4a), each pearl oyster is transplanted and each transplantation marked by predetermined date, namely 22-06-2009 and on harvest date 30-09-201 0 with silver pearl product measuring 0.5mm to 10mm can be found as showed in picture 4b, silver and white pearl product in varying sizes from 0.25mm to 10mm having the contents of COC0 3 of 80.82% weight, Mg C0 3 of 2.16% weight, Ca 3 (P0 4 ) 2 of 0.16% weight, Si0 2 of 9.54% weight, the rest are Al 2 O3 + Fe 2 03 with the temperature under H OC 0 with evaporated H 2 0 of 13.44% weight and decrease around 30-40% of total pearl oyster planted and after harvest they have varying sizes with the process to produce cultured pearl product through transplantation by using natural pearl waste in accordance with this invention.
- Sample is taken from U-9 through the process to produce cultured pearl product with transplantation by using natural pearl waste in the form of irregular elliptical through pre-registered transplantation process with total 120 pearl oysters (picture 5a), each pearl oyster is transplanted and each transplantation marked by predetermined date, namely 22-06-2009 and on harvest date of 30-09-201 0 with silver pearl product measuring 0.5mm to 1 1 mm can be found as showed in picture 5b, silver and white pearl product in varying sizes from 0.25mm to 1 0mm having the contents of COCO 3 of 80.82% weight, Mg C0 3 of 2.16% weight, Ca 3 (P0 4 ) 2 of 0.16% weight, Si0 2 of 9.54% weight, the rest are Al 2 0 3 + Fe 2 03 with the temperature under H OC 0 with evaporated H 2 0 of 13.44% weight and decrease around 30-40% of total pearl oyster planted and after harvest they have varying sizes with the process to produce cultured pearl product through transplantation by using natural pearl waste in accordance with this invention.
- the first inventor provides a process to produce pearl product by using natural pearl waste with the following steps;
- Epithelium cell in the form of small slices that are mixed with Sodium chloride solution 0.90% injected until it touches outer wall of nucleus in gonadal position in pearl oyster body.
- inventor provides a pearl product to produce cultured pearl product through transplantation by using natural pearl waste with the contents of COCO 3 of 80.82% weight, Mg C0 3 of 2.16% weight, Ca 3 (P0 4 ) 2 of 0. 16% weight, Si0 2 of 9.54% weight, the rest are Al 2 0 3 + Fe 2 03 with the temperature under H OC 0 with evaporated H 2 0 of 13.44% weight.
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
Abstract
This invention is relating to a process to produce cultured pearl through transplantation by using natural pearl waste, with silver pearl product contains COCO3 of 80.82% weight, Mg CO3 of 2.16% weight, Ca3 (P04)2 of 0.16% weight, SiO2 of 9.54% weight, the rest are AI203 + Fe2 O3 with the temperature under H OC0 with evaporated H2O of 13.44% weight. By applying injection of softened epithelium cell to become small cuts injected into gonadal cavity by using sodium chloride solution 0.90% to touch surface natural pearl nucleus as its transplanter. (* *)
Description
Description
Process to produce cultured pearl product through transplantation by using natural pearl nucleus waste
Field of Invention Engineering
This invention is generally relating to a process to produce cultured pearl product through transplantation by using natural pearl waste, more specifically the process to produce cultured pearl product through transplantation by using natural pearl waste with injection of softened epithelium cell to become a small cut which is injected with 0.90% sodium chloride solution into gonadal cavity to touch the surface of natural pearl nucleus as its transplant.
Background of Invention
As we know that Indonesia has good coastal waters and marine areas to cultivate pearl oyster, and the product obtained from natural oyster that has been explored for years, in which products of such manners, so many natural oyster are obtained and most of natural pearls become waste since their form are not compliant with standards, thus they are unsold.
Based on such matters, inventor tries to utilize waste of unsold natural pearl grains and that become waste. Based on the problem mentioned above the inventor tries to make a waste of natural pearl grain as transplant.
As conducted previously, during transplantation on pearl culture, namely by using nucleus in the form of rounded grain with certain shape so that by using frame with open oyster shell by making an epithelium sell through slicing leg' s part of such frame, so that it reaches gonadal part, a layer of oyster reproduction area. Such matter has been revealed previously in currently existing pearl culture.
Nucleus user in pearl oyster culture made of pearl grain which is created in Arizona, nearby Mississippi River so that such grains are used as nuclei in pearl transplantation.
Transplantation function as we know that by implanting grains or nuclei existing inside pearl body within long period of time, then such nuclei are coated by resin produced by pearl body and the longer the time the more harden and coat the whole nuclei so that it is obtained cultured pearl within relatively short period from 1 .5 years to 2 years to obtain pearl product by harvesting such pearls.
And insufficiency and weakness of pearl product using nucleus made of grain of oyster shell powder taken and shaped to obtain such grain is pearls product obtained after harvest is poor and not as good as genuine natural pearl, in which pearl sparkle is different from cultured pearl.
Based on such matter, then inventor tries to obtain pearl culture with products and quality of pearl approaching natural original pearl
by making transplantation from the product of natural pearl waste that has been explored for years by some of people, in which the shape of original pearl with varying sizes which are still has not obtained yet intended pearl shape, in which in Indonesia has original pearl reserves without standard size as pearls in demand for sale and still in unfinished form, so that pearl price is low or completely not interested in by pearl lovers.
By utilizing this original pearl waste as pearl nucleus by using process to make pearl culture product from original pearls waste material among others by considering the following factors:
The first factor, inventor considers that I Indonesia has supporting natural geography to obtain pearl that has been explores within long period of time, in which in searching natural pearl there were so many damaged pearl oyster, that eventually it become scarce year by year, then in such search there is always any pearl waste left that so far will stack to become natural pearl waste material, in which it has non standard shape with smaller size of pearl feasible to become pearl ornamental material, those all become waste since such materials are unsold to trade.
The second factor, inventor tries to make process to produce pearl culture by nucleus transplant made of natural pearl waste so that it boosts benefit of unmarketable natural pearl waste to marketable pearl object through pearl culture with special process such as
treating pearl oyster to obtain pearl grain waste to become pearl close to original pearl.
The third factor, inventor provides a process to produce pearl culture through transplantation with natural pearl waste nucleus by providing special equipments and treatment with different process from pearl culture as existing previously.
The fourth factor, inventor provides a process to make pearl culture product through nucleus transplantation by using natural pearl waste since pearl waste that has been stacked for years in Indonesia as a result of exploration of unusable waste into valuable and value added pearl product, a process of making pearl culture from the nucleus transplant of pearl waste.
The fifth factor is to make a pearl getting close to natural pearl by adding values from valueless pearl waste to high valuable pearl.
The sixth factor is to make use of pearl waste owned by Indonesia in order to be re-utilized into feasibly-sold pearl by culturing pearl that uses nucleus transplanted into oyster pearl to be made cultured pearl.
The seventh factor is to use existing pearl waste by applying process to implement nucleus in use as a grain implanted into cultured oyster pearl that eventually produces pearl from natural pearl waste to be made high-quality pearl or equivalent to natural pearl quality.
Based on above existing factor, then inventor tries to make a process to produce pearl by using natural pearl waste as a nucleus in order to make good and high-quality natural pearl culture by considering the following aspects:
The first aspect, inventor provides a process to make pearl by using natural pearl as nucleus to be transplanted into oyster pearl as pearl culture oyster to produce high quality pearl.
The second aspect, inventor provides a process to make pearl product by using natural pearl waste with the following steps:
- the first step, to provide pearl oyster that has been bred for the preparation of opening each oyster shell and examine health of pearl oyster;
- the second step, to prepare nucleus by taking natural pearl waste with various sizes of grain;
- the third step, to cleanse natural pearl grain by using alcohol 70 to 95%;
- the fourth step, to rinse nucleus cleanly with sodium chloride solution 0.90%;
- the fifth step, to sterilize by mixing rinsing substance and antibiotics;
- the sixth step, to insert nucleus into oyster body;
- the seventh step, to slice leg's part until gonadal tissues (oyster reproduction area);
- the eight step, to enter pearl nucleus through cavity of slicing on gonadal layer;
- to enter nucleus through gonadal cavity
- to inject epithelium cell;
are characterized by:
Epithelium cell in the form of cell of small slice mixed with Sodium Chloride solution 0.90% which is injected until it touches outer wall of nucleus on gonadal position on pearl oyster body.
The fourth aspect, inventor provides an equipment to produce pearl by using natural pearl waste with the following equipment composition;
Equipment to produce pearl by using natural pearl waste as nucleus implanted into culture pearl oyster through epithelium cell injection process by using injection equipment having predetermined epithelium cell dosage. It aims at epithelium cell can touch surface of nucleus outer layer of pearl oyster waste in pearl culture oyster body.
The term "epithelium cell" is epithelium cell taken out from blended or destroyed oyster body to become refined form to be mixed with sodium chloride solution 0.90% to obtain diluted solution thus it can be injected into body cultured pearl tissues easily through slicing hole in pearl body to reach gonad (or oyster reproduction area) with size ratio of 50: 50 in order to be injected to gonadal cavity on sliced
oyster body to touch outer skin surface of such nucleus, thus nucleus in body is eventually coated by body fluid from enzyme produced by oyster body to change into pearl.
Term of "injection" is an injection facility to distribute fluid in the form of oyster epithelium cell which can accelerate the formation of pearl layer in oyster body by way of injection.
The term of "natural oyster waste" is semi-finished pearl grains produced by valueless natural pearl oyster that becoming waste with smaller diameter than standard of pearl as jewelry. And various forms of grain and color, from white to black, waste pearl produced from pearl exploitation is obtained unusable grains as pearl material for jewelry.
The term of "nucleus" is material to produce a pearl by implanting it in oyster body through slicing and put nucleus into gonad tissues layer.
Brief Description of Invention
The objective of this invention is to provide process to produce pearl by using natural pearl waste as nucleus to be transplanted into pearl oyster as pearl culture oyster to produce high quality pearl.
Other objective of this invention is to provide a process to produce a pearl by using nucleus and valueless natural pearl waste to become valuable one.
Other objective of this invention is to provide a process to produce pearl product by using natural pearl waste with the following steps;
- the first step, to provide pearl oyster that has been bred for the preparation by opening each oyster shell and examines health of pearl oyster,
- the second step, to prepare nucleus by taking natural pearl waste with various sizes of grain,
- the third step, to cleanse natural pearl grain by using alcohol 70% to 95%,
- the fourth step, to rinse nucleus cleanly with sodium chloride solution 0.90%
- the fifth step, to sterilize by mixing rinsing material and antibiotics;
- the sixth step, to insert nucleus into oyster body;
- the seventh step, to slice leg's part until gonadal tissues;
- the eighth step, to enter nucleus of pearl through slicing path of gonadal layer;
- to put nucleus into gonadal cavity
- to inject epithelium cell, characterized by
Epithelium cell in the form of small cuts mixed with Sodium chloride solution 0.90% which is injected until it touches outer wall of nucleus
on gonadal position in pearl oyster body.
Further objective of this invention is to provide an equipment to produce pearl by using natural pearl waste with the following formation of equipments;
Equipment to produce pearl by using natural pearl waste as nucleus which is implanted into cultured pearl oyster by using epithelium cel l injection process by applying injection equipment having predetermined epithelium cell dosage in order that epithelium cell can touch outer layer surface of pearl oyster waste nucleus in body of pearl culture oyster.
Brief Description of Picture
To clarify the essence of this invention, by referring to realization of the following picture;
Picture l a demonstrates a picture of U-5 from natural bred pearl grain and picture l b is a picture of transplanted pearl product after being harvested with the process to produce cultured pearl product by transplantation by using natural pearl waste.
Picture 2a demonstrates the picture of U-6 from cultured natural pearl grain and Picture 2b is a picture of transplanted pearl product after being harvested with the process to produce cultured pearl product through transplantation by using natural pearl waste.
Picture 3a demonstrates the picture of U-7 of cultured natural pearl grain and Picture 3b is a picture of transplanted pearl product after being harvest with the process to produce cultured pearl through transplantation by using natural pearl waste.
Picture 4a demonstrates the picture of U-8 of cultured natural pearl grain and Picture 4b is a picture of transplanted pearl product after being harvested with the process to produce cultured pearl product through transplantation by using natural pearl waste.
Picture 5 a demonstrates the picture of U-9 of cultured natural pearl grain and Picture %b is a picture of transplanted pearl product after being harvested with the process to produce cultured pearl through transplantation by using natural pearl waste.
Full Description of Invention
As explained previously on method of transplanting oyster by putting nucleus into oyster body by using the following preparations:
Procedures to prepare nucleus for nucleus insertion process into cultured pearl body.
Firstly, to take out oyster from the sea or breeding area,
Secondly, to open by one the oysters which have already examined their health, from the aspect of color, shape of gonad and their health.
Thirdly, to take out oysters to be open their shells and put a wedge in
order to open oyster shells and observe body of oyster.
Procedure to Prepare Nucleus
Firstly, nucleus is washed with alcohol 70% to 95%, in order that nucleus made of natural pearl waste is clean and free from contamination.
Secondly, to rinse nucleus by using sterile sodium chloride solution 0.90% to remove alcohol sticking to surface of natural pearl waste grain.
Thirdly, to mix with antibiotics in rinsing solution (tetracycline, penicillin, or betandienn) after all nuclei are clean.
The last is
Insertive process is to prepare nucleus into cultured oyster body that has been prepared in step on procedures to prepare nucleus for nucleus insertive process.
With the following steps
To make epithelium cell
The second step is to slice leg' s part until it reaches gonadal layer (oyster reproduction area).
10mm to 5mm
3 U-7 6-5mm Oval 612 22-08- 30-09- Silver and grains 2009 2010 black pearl product measuring 3mm to 5mm
4 U-8 5-3mm Round 205 22-06- 30-09- Silver pearl grains 2009 2010 product
measuring
5mm to l 1 mm
5 U-9 3-2mm Round 120 22-06- 30-09- Silver pearl grains 2009 2010 product
measuring
5mm to l 1 mm
Transplantation is marked by predetermined date, namely 16-06-2009 and harvest day of 16-06-2010 with the silver pearl product measuring 0.5mm to 10mm, such dimension can be found as showed in Picture2b silver and white pearl product in various sizes of 0.25mm to 10 mm
which have the contents of COC03 of 80.82% weight Mg C03 of 2.16% weight, Ca3 (P04)2 of 0.16% weight, Si02 of 9.54% weight, the rest are Al2 O3 + Fe2 03 with the temperature under H OC0 with evaporated H20 of 13.44% weight and decrease around 30-40% from the total pearl oyster implanted after harvest they have varying sizes with the process to produce cultured pearl product through transplantation by using natural pearl waste in accordance with this invention.
Sample 3
Sample is taken from U-7 through the process to produce cultured pearl product through transplantation by using natural pearl waste in the form of irregular elliptical through pre-registered transplantation process with total 612 pearl oysters (Picture 3 a), each pearl oyster is transplanted and each transplantation marked by predetermined date, namely 22-06-2009 and on harvest date of 30-09-2010 with silver pearl product measuring 0.5mm to 10mm can be found as showed in picture 3b, silver and white pearl product in varying sizes from 0.25mm to 10mm having the contents of COC03 of 80.82% weight, Mg C03 of 2.16% weight, Ca3 (P04)2 of 0.16% weight, Si02 of 9.54% weight, the rest are Al2 O3 + Fe2 03 with the temperature under 1 10C° with evaporated H20 of 13.44% weight and decrease around 30-40% of total pearl oyster implanted and after harvest they have varying sizes with the process to produce cultured pearl product through
transplantation by using natural pearl waste in accordance with this invention.
Sample 4
Sample is taken from U-8 through the process to produce cultured pearl product through transplantation by using natural pearl waste in the form of irregular elliptical through pre-registered transplantation process with total 205 pearl oysters (picture 4a), each pearl oyster is transplanted and each transplantation marked by predetermined date, namely 22-06-2009 and on harvest date 30-09-201 0 with silver pearl product measuring 0.5mm to 10mm can be found as showed in picture 4b, silver and white pearl product in varying sizes from 0.25mm to 10mm having the contents of COC03 of 80.82% weight, Mg C03 of 2.16% weight, Ca3 (P04)2 of 0.16% weight, Si02 of 9.54% weight, the rest are Al2 O3 + Fe2 03 with the temperature under H OC0 with evaporated H20 of 13.44% weight and decrease around 30-40% of total pearl oyster planted and after harvest they have varying sizes with the process to produce cultured pearl product through transplantation by using natural pearl waste in accordance with this invention.
Sample 5
Sample is taken from U-9 through the process to produce cultured pearl product with transplantation by using natural pearl waste in the
form of irregular elliptical through pre-registered transplantation process with total 120 pearl oysters (picture 5a), each pearl oyster is transplanted and each transplantation marked by predetermined date, namely 22-06-2009 and on harvest date of 30-09-201 0 with silver pearl product measuring 0.5mm to 1 1 mm can be found as showed in picture 5b, silver and white pearl product in varying sizes from 0.25mm to 1 0mm having the contents of COCO3 of 80.82% weight, Mg C03 of 2.16% weight, Ca3 (P04)2 of 0.16% weight, Si02 of 9.54% weight, the rest are Al2 03 + Fe2 03 with the temperature under H OC0 with evaporated H20 of 13.44% weight and decrease around 30-40% of total pearl oyster planted and after harvest they have varying sizes with the process to produce cultured pearl product through transplantation by using natural pearl waste in accordance with this invention.
Based on the results from sample 1 to sample 5, it is produced original pearl with the contents of COC03 of 80.82% weight, Mg C03 of 2.16% weight, Ca3 (P04)2 of 0. 16% weight, Si02 of 9.54% weight, the rest are Al2 03 + Fe2 03 with the temperature under 1 10C° with evaporated H20 of 13.44% weight. It is decrease from early and final result due to weather factor and temperature around pearl oyster habitat, thus make some of them died. The result of dead pearl waste can be reused existing nucleus to be bred into cultured pearl through transplantation by using natural pearl waste in accordance with this
invention.
Conclusion
The first inventor provides a process to produce pearl product by using natural pearl waste with the following steps;
- the first step, to provide pearl oyster that has been bred to be prepared buy opening each oyster shell and examines health of pearl oyster;
- the second step, to prepare nucleus by taking natural pearl waste with various sizes of grain from 2.5mm to 10mm;
- the third step, to cleanse natural pearl grain by using alcohol 70 to 95%;
- the fourth step, to rinse nucleus cleanly with sodium chloride solution 0.90%;
- the fifth step, to sterilize by mixing rinsing substance and antibiotics;
- the sixth step, to insert nucleus into oyster body;
- the seventh step, to slice leg' s part until gonadal tissues (oyster reproduction area) tissue;
- the eight step, to put pearl nucleus through slicing path on gonadal layer;
- to enter nucleus through gonadal cavity
- to inject epithelium cell;
characterized by
Epithelium cell in the form of small slices that are mixed with Sodium chloride solution 0.90% injected until it touches outer wall of nucleus in gonadal position in pearl oyster body.
Secondly, inventor provides a pearl product to produce cultured pearl product through transplantation by using natural pearl waste with the contents of COCO3 of 80.82% weight, Mg C03 of 2.16% weight, Ca3 (P04)2 of 0. 16% weight, Si02 of 9.54% weight, the rest are Al2 03 + Fe2 03 with the temperature under H OC0 with evaporated H20 of 13.44% weight.
Modification and variation conducted by the expert in his/her field, including transplantation with original pearl grain which are not suitable for standard of pearl size for sale or natural pearl product, it is still encompassed in scope and the essence in this invention as claims attached as follows;
Claims
1 . A process to produce pearl product by using natural pearl waste with the following steps;
- to provide pearl oyster that has been bred for the preparation by opening each oyster shell and examines health of pearl oyster;
- to prepare nucleus by taking natural pearl waste with various sizes of grain from 2.5mm to 10mm;
- to cleanse natural pearl grain by using alcohol 70% to 95%,
- to rinse nucleus cleanly with sodium chloride solution 0.90%
- to sterilize by mixing rinsing material and antibiotics;
- to insert nucleus into oyster body;
- to slice leg's part until gonadal tissues;
- to put pearl nucleus through gonadal cavity;
- to inject epithelium cell;
Characterized by,
Epithelium cell in the form of small slices that are mixed with Sodium chloride solution 0.90% injected until it touches outer wall of nucleus in gonadal position in pearl oyster body.
2. Pearl product produced through the process to make cultured pearl product through transplantation by using natural pearl waste as nucleus as claimed in claim 1 , in which silver pearl product contains COC03 of 80.82% weight, Mg C03 of 2.16% weight, Ca3 (P04)2 of 0. 16% weight, Si02 of 9.54% weight, the rest are A1203 + Fe2 03 with the temperature under H OC0 with evaporated H20 of 1 3.44% weight.
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| CN2011800219812A CN102869245A (en) | 2010-12-02 | 2011-11-10 | Process to produce cultured pearl product through transplantation by using natural pearl nucleus waste |
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| IDP00201000814 | 2010-12-02 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935249A (en) * | 2018-08-03 | 2018-12-07 | 广东荣辉珍珠养殖有限公司 | Pearl breedinmg natural peral method and gained pearl |
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| CN103355229B (en) * | 2013-06-06 | 2015-01-07 | 金华职业技术学院 | Immunostimulation generation method of pearl sac of pearl mussel |
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|---|---|---|---|---|
| US4783975A (en) * | 1986-12-17 | 1988-11-15 | Catalysts & Chemicals Industries Co., Ltd. | Colored pearl |
| JPH03183424A (en) * | 1989-12-11 | 1991-08-09 | Pearl Res Center:Kk | Production of nucleus for sustained release of antibiotic substance for culturing pearl |
| JPH08336342A (en) * | 1995-06-12 | 1996-12-24 | Toyoda Shinjiyukaku Kogyosho:Kk | Production of pearl seed |
| US20070193526A1 (en) * | 2006-02-17 | 2007-08-23 | Batzer William B | Pearl culture method and product |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86101659A (en) * | 1986-02-03 | 1987-01-24 | 湖南省汉寿县特种水产研究所 | Operation method of breeding large combined pearls |
| CN1163120C (en) * | 2000-06-20 | 2004-08-25 | 谢绍河 | Pearl seed inserting method for pearl culture in fresh water mussel |
| CN1139321C (en) * | 2000-09-22 | 2004-02-25 | 谢绍河 | Whole-nacre nucleus pearl and its culture method |
-
2011
- 2011-11-10 WO PCT/ID2011/000006 patent/WO2012073226A2/en active Application Filing
- 2011-11-10 CN CN2011800219812A patent/CN102869245A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4783975A (en) * | 1986-12-17 | 1988-11-15 | Catalysts & Chemicals Industries Co., Ltd. | Colored pearl |
| JPH03183424A (en) * | 1989-12-11 | 1991-08-09 | Pearl Res Center:Kk | Production of nucleus for sustained release of antibiotic substance for culturing pearl |
| JPH08336342A (en) * | 1995-06-12 | 1996-12-24 | Toyoda Shinjiyukaku Kogyosho:Kk | Production of pearl seed |
| US20070193526A1 (en) * | 2006-02-17 | 2007-08-23 | Batzer William B | Pearl culture method and product |
Non-Patent Citations (2)
| Title |
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| A.C.C. VICTOR ET AL.: 'Pearl oyster farming and pearl production' BULL. CENT. MAR. FISH. RES. INST. vol. 48, 1996, pages 56 - 63 * |
| MARIA HAWS: ''The Basics of Pearl Farming: A Layman' s Manual'' CENTER FOR TROPICAL AND SUBTROPICAL AQUACULTURE no. 127, March 2002, pages 51 - 66 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935249A (en) * | 2018-08-03 | 2018-12-07 | 广东荣辉珍珠养殖有限公司 | Pearl breedinmg natural peral method and gained pearl |
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| WO2012073226A3 (en) | 2012-10-04 |
| CN102869245A (en) | 2013-01-09 |
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