WO2012029224A8 - 標的物質の検出方法、それに用いるアプタマーセット並びにセンサ及び装置 - Google Patents

標的物質の検出方法、それに用いるアプタマーセット並びにセンサ及び装置 Download PDF

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Publication number
WO2012029224A8
WO2012029224A8 PCT/JP2011/003486 JP2011003486W WO2012029224A8 WO 2012029224 A8 WO2012029224 A8 WO 2012029224A8 JP 2011003486 W JP2011003486 W JP 2011003486W WO 2012029224 A8 WO2012029224 A8 WO 2012029224A8
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Prior art keywords
aptamer
target substance
double
sensor
used therefor
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PCT/JP2011/003486
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English (en)
French (fr)
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WO2012029224A1 (ja
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公康 田光
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日本電気株式会社
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Publication date
Application filed by 日本電気株式会社 filed Critical 日本電気株式会社
Priority to JP2012531658A priority Critical patent/JP5803923B2/ja
Priority to CN2011800424465A priority patent/CN103108952A/zh
Priority to US13/820,015 priority patent/US20130157279A1/en
Priority to EP11821249.7A priority patent/EP2612910A4/en
Publication of WO2012029224A1 publication Critical patent/WO2012029224A1/ja
Publication of WO2012029224A8 publication Critical patent/WO2012029224A8/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

標的物質の検出方法は、被検用試料中の標的物質（５）が特異的に結合する第１のアプタマー（１）と標的物質（５）以外の第２のリガンド６が特異的に結合する第２のアプタマー（２）とを備え、第１のアプタマー（１）は１以上の二本鎖が形成する二本鎖形成部位を有しており、第２のアプタマー（２）は２以上の二本鎖形成部位を有しており、標的物質（５）が結合する第１のアプタマー１の塩基配列は、第１のアプタマー１の二本鎖形成部位の塩基配列の一部を少なくとも含み、第２のリガンド（６）が結合する第２のアプタマー２の塩基配列は、第２のアプタマー（２）の２以上の二本鎖形成部位の塩基配列の一部を少なくとも含む、複合体（４）を用意する工程と、標的物質（５）と第１のアプタマー（１）とを結合させる工程と、第２のリガンド（６）と第２のアプタマー２とを結合させる工程と、第１のアプタマー１の二本鎖形成部位の開裂を検出する工程と、を含む。
PCT/JP2011/003486 2010-09-01 2011-06-17 標的物質の検出方法、それに用いるアブタマーセット並びにセンサ及び装置 WO2012029224A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2012531658A JP5803923B2 (ja) 2010-09-01 2011-06-17 標的物質の検出方法、それに用いるアプタマーセット並びにセンサ及び装置
CN2011800424465A CN103108952A (zh) 2010-09-01 2011-06-17 检测目标物质的方法以及用于所述方法中的适体组、传感器和装置
US13/820,015 US20130157279A1 (en) 2010-09-01 2011-06-17 Method for detecting target substance and aptamer set, sensor, and apparatus used in the method
EP11821249.7A EP2612910A4 (en) 2010-09-01 2011-06-17 METHOD FOR DETECTING A TARGET SUBSTANCE, USING THE APTAMER SET AND SENSOR AND DEVICE THEREFOR

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2010196053 2010-09-01
JP2010-196053 2010-09-01

Publications (2)

Publication Number Publication Date
WO2012029224A1 WO2012029224A1 (ja) 2012-03-08
WO2012029224A8 true WO2012029224A8 (ja) 2012-06-28

Family

ID=45772351

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PCT/JP2011/003486 WO2012029224A1 (ja) 2010-09-01 2011-06-17 標的物質の検出方法、それに用いるアブタマーセット並びにセンサ及び装置

Country Status (5)

Country Link
US (1) US20130157279A1 (ja)
EP (1) EP2612910A4 (ja)
JP (1) JP5803923B2 (ja)
CN (1) CN103108952A (ja)
WO (1) WO2012029224A1 (ja)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013147217A1 (ja) 2012-03-30 2013-10-03 国立大学法人九州大学 センサ、検出方法、検出システム、及び、検出装置
KR101974577B1 (ko) * 2012-05-21 2019-05-02 삼성전자주식회사 나노입자 제작용 주형 및 이를 이용한 나노입자의 제조 방법
US9310357B2 (en) * 2012-09-28 2016-04-12 Src, Inc. Detection of chemical and biological agents using oligonucleotide aptamers
WO2016157027A1 (en) * 2015-04-03 2016-10-06 Bettinsoli Luca Apparatus and method for a separation through magnetic nanoparticles
WO2018067521A1 (en) * 2016-10-03 2018-04-12 Eccrine Systems, Inc. Biosensors with ph-independent redox moieties
CN110568175A (zh) * 2019-09-06 2019-12-13 成都理工大学 一种基于核酸染料诱导纳米金快速聚集检测dna的方法
JP7418274B2 (ja) * 2020-04-17 2024-01-19 東京エレクトロン株式会社 異物検査システム、異物検査方法、プログラム及び半導体製造装置
WO2022260463A1 (ko) * 2021-06-10 2022-12-15 주식회사 제우스 진단키트 및 진단 방법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6686150B1 (en) * 1998-01-27 2004-02-03 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
EP1183521B1 (en) * 1999-05-14 2014-03-19 Brandeis University Aptamer-based detection
WO2006093892A2 (en) * 2005-02-28 2006-09-08 Gen-Probe Incorporated Compositions and methods of detecting an analyte by using a nucleic acid hybridization switch probe
US7892734B2 (en) 2005-08-11 2011-02-22 The Board Of Trustees Of The University Of Illinois Aptamer based colorimetric sensor systems
WO2007032359A1 (ja) * 2005-09-12 2007-03-22 National University Corporation Tokyo University Of Agriculture And Technology アプタマー・プローブ複合体を用いた標的分子の検出方法
JP5317221B2 (ja) * 2008-04-01 2013-10-16 日本電気株式会社 アプタマー選抜方法及び装置、ならびにアプタマーを用いたセンサ方法
JP2010196053A (ja) 2009-01-30 2010-09-09 Sekisui Plastics Co Ltd ポリオレフィン系樹脂フィルム及び積層シート
WO2010107058A1 (ja) * 2009-03-17 2010-09-23 日本電気株式会社 標的物質の検出方法

Also Published As

Publication number Publication date
EP2612910A4 (en) 2015-05-06
EP2612910A1 (en) 2013-07-10
JPWO2012029224A1 (ja) 2013-10-28
CN103108952A (zh) 2013-05-15
JP5803923B2 (ja) 2015-11-04
WO2012029224A1 (ja) 2012-03-08
US20130157279A1 (en) 2013-06-20

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