WO2012002450A1 - D-succinylase et procédé de production d'un acide d-aminé l'utilisant - Google Patents

D-succinylase et procédé de production d'un acide d-aminé l'utilisant Download PDF

Info

Publication number
WO2012002450A1
WO2012002450A1 PCT/JP2011/064943 JP2011064943W WO2012002450A1 WO 2012002450 A1 WO2012002450 A1 WO 2012002450A1 JP 2011064943 W JP2011064943 W JP 2011064943W WO 2012002450 A1 WO2012002450 A1 WO 2012002450A1
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
succinyl
succinylase
protein
seq
Prior art date
Application number
PCT/JP2011/064943
Other languages
English (en)
Japanese (ja)
Inventor
伸弥 熊谷
西矢　芳昭
幸夫 岩井
洋輔 角田
Original Assignee
積水メディカル株式会社
東洋紡績株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 積水メディカル株式会社, 東洋紡績株式会社 filed Critical 積水メディカル株式会社
Priority to JP2012522670A priority Critical patent/JP5806664B2/ja
Publication of WO2012002450A1 publication Critical patent/WO2012002450A1/fr

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
    • C12P41/007—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures by reactions involving acyl derivatives of racemic amines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

La présente invention concerne une D-succinylase inédite présentant une forte activité hydrolytique sur l'acide N-succinyl-D-aminé et se montrant capable de produire de l'acide D-aminé avec un bon rendement à l'échelle industrielle et dans des délais adaptés à une production commerciale lorsqu'elle est combinée au racémase d'un acide aminé succinylique. L'invention concerne, plus précisément, une protéine qui est caractérisée en ce qu'elle peut être l'une des quatre protéines (a) à (d) suivantes : (a) une protéine encodée par un gène comprenant la séquence nucléotidique présentée par les SEQ ID NO : 2, 4, 6 ou 8 ; (b) une protéine comprenant la séquence d'acides aminés présentée par les SEQ ID NO : 1, 3, 5 ou 7 ; (c) une protéine dotée de l'activité D-succinylase et encodée par un polynucléotide capable de s'hybrider avec une séquence nucléotidique complémentaire de la séquence nucléotidique présentée par les SEQ ID NO : 2, 4, 6 ou 8 dans des conditions de stringence ; et (d) une protéine dotée d'une activité D-succinylase et comprenant une séquence d'acides aminés obtenue par substitution, délétion, insertion et/ou addition d'un ou plusieurs acides aminés dans la protéine contenant la séquence d'acides aminés présentée par les SEQ ID NO : 1, 3, 5 ou 7.
PCT/JP2011/064943 2010-06-30 2011-06-29 D-succinylase et procédé de production d'un acide d-aminé l'utilisant WO2012002450A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2012522670A JP5806664B2 (ja) 2010-06-30 2011-06-29 Ｄ−サクシニラーゼ、およびこれを用いたｄ−アミノ酸の製造方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2010148795 2010-06-30
JP2010-148795 2010-06-30
JP2010196881 2010-09-02
JP2010-196881 2010-09-02

Publications (1)

Publication Number Publication Date
WO2012002450A1 true WO2012002450A1 (fr) 2012-01-05

Family

ID=45402154

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2011/064943 WO2012002450A1 (fr) 2010-06-30 2011-06-29 D-succinylase et procédé de production d'un acide d-aminé l'utilisant

Country Status (2)

Country Link
JP (4) JP5806664B2 (fr)
WO (1) WO2012002450A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012124513A1 (fr) * 2011-03-14 2012-09-20 東洋紡績株式会社 D-succinylase modifiée ayant une sélectivité pour la forme d améliorée pour l'acide n-succinyl-dl-aminé
US9700599B2 (en) 2012-11-13 2017-07-11 Adocia Rapid-acting insulin formulation comprising a substituted anionic compound
US9795678B2 (en) 2014-05-14 2017-10-24 Adocia Fast-acting insulin composition comprising a substituted anionic compound and a polyanionic compound
WO2018088434A1 (fr) * 2016-11-10 2018-05-17 東洋紡株式会社 Procédé de production d'acide n-succinyl-hydroxy-d-amino et/ou d'acide hydroxy-d-amino
US10525133B2 (en) 2014-05-14 2020-01-07 Adocia Aqueous composition comprising at least one protein and one solubilizing agent, preparation thereof and uses thereof
US10792335B2 (en) 2015-11-16 2020-10-06 Adocia Rapid-acting insulin composition comprising a substituted citrate

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008061642A (ja) * 2006-08-10 2008-03-21 Toyobo Co Ltd Ｄ−アミノ酸の製造方法
JP2008307006A (ja) * 2007-06-15 2008-12-25 Toyobo Co Ltd Ｌ−アミノ酸の製造方法
WO2009136500A1 (fr) * 2008-05-07 2009-11-12 東洋紡績株式会社 L-succinyl aminoacylase et procédé de production d'un acide l-amino au moyen de cette dernière
WO2010067613A1 (fr) * 2008-12-11 2010-06-17 東洋紡績株式会社 L-succinyle aminoacylase et procédé pour produire un acide aminé l en utilisant celle-ci

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008061642A (ja) * 2006-08-10 2008-03-21 Toyobo Co Ltd Ｄ−アミノ酸の製造方法
JP2008307006A (ja) * 2007-06-15 2008-12-25 Toyobo Co Ltd Ｌ−アミノ酸の製造方法
WO2009136500A1 (fr) * 2008-05-07 2009-11-12 東洋紡績株式会社 L-succinyl aminoacylase et procédé de production d'un acide l-amino au moyen de cette dernière
WO2010067613A1 (fr) * 2008-12-11 2010-06-17 東洋紡績株式会社 L-succinyle aminoacylase et procédé pour produire un acide aminé l en utilisant celle-ci

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SAKAI A. ET AL.: "Evolution of Enzymatic Activities in the Enolase Superfamily: N-Succinylamino Acid Racemase and a New Pathway for the Irreversible Conversion of D- to L-Amino Acids", BIOCHEMISTRY, vol. 45, 2006, pages 4455 - 4462 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012124513A1 (fr) * 2011-03-14 2012-09-20 東洋紡績株式会社 D-succinylase modifiée ayant une sélectivité pour la forme d améliorée pour l'acide n-succinyl-dl-aminé
US9700599B2 (en) 2012-11-13 2017-07-11 Adocia Rapid-acting insulin formulation comprising a substituted anionic compound
US10583175B2 (en) 2012-11-13 2020-03-10 Adocia Rapid-acting insulin formulation comprising a substituted anionic compound
US10646551B2 (en) 2012-11-13 2020-05-12 Adocia Rapid-acting insulin formulation comprising a substituted anionic compound
US10881716B2 (en) 2012-11-13 2021-01-05 Adocia Rapid-acting insulin formulation comprising a substituted anionic compound
US11324808B2 (en) 2012-11-13 2022-05-10 Adocia Rapid-acting insulin formulation comprising a substituted anionic compound
US9795678B2 (en) 2014-05-14 2017-10-24 Adocia Fast-acting insulin composition comprising a substituted anionic compound and a polyanionic compound
US10525133B2 (en) 2014-05-14 2020-01-07 Adocia Aqueous composition comprising at least one protein and one solubilizing agent, preparation thereof and uses thereof
US10792335B2 (en) 2015-11-16 2020-10-06 Adocia Rapid-acting insulin composition comprising a substituted citrate
WO2018088434A1 (fr) * 2016-11-10 2018-05-17 東洋紡株式会社 Procédé de production d'acide n-succinyl-hydroxy-d-amino et/ou d'acide hydroxy-d-amino
JPWO2018088434A1 (ja) * 2016-11-10 2019-10-03 東洋紡株式会社 Ｎ−サクシニル−ヒドロキシ−ｄ−アミノ酸及び／又はヒドロキシ−ｄ−アミノ酸の製造方法
JP6996714B2 (ja) 2016-11-10 2022-01-17 東洋紡株式会社 Ｎ－サクシニル－ヒドロキシ－ｄ－アミノ酸及び／又はヒドロキシ－ｄ－アミノ酸の製造方法

Also Published As

Publication number Publication date
JP6048851B2 (ja) 2016-12-21
JP5806664B2 (ja) 2015-11-10
JP6048850B2 (ja) 2016-12-21
JP2015198666A (ja) 2015-11-12
JPWO2012002450A1 (ja) 2013-08-29
JP6044675B2 (ja) 2016-12-14
JP2015213506A (ja) 2015-12-03
JP2015221037A (ja) 2015-12-10

Similar Documents

Publication Publication Date Title
JP6048850B2 (ja) Ｄ−サクシニラーゼ、およびこれを用いたｄ−アミノ酸の製造方法
US7316916B2 (en) DNA for encoding D-hydantoin hydrolases, DNA for encoding N-carbamyl-D-amino acid hydrolases, recombinant DNA containing the genes, cells transformed with the recombinant DNA, methods for producing proteins utilizing the transformed cells and methods for producing D-amino acids
JP2008061642A (ja) Ｄ−アミノ酸の製造方法
US7314738B2 (en) DNA for encoding D-hydantoin hydrolases, DNA for encoding N-carbamyl-D-amino acid hydrolases, recombinant DNA containing the genes, cells transformed with the recombinant DNA, methods for producing proteins utilizing the transformed cells and methods for producing D-amino acids
JP5903298B2 (ja) Ｎ−サクシニル−ｄｌ−アミノ酸に対する向上されたｄ体選択性を有する改変型ｄ−サクシニラーゼ
WO2006062189A1 (fr) Transformant exprimant la nitrile hydratase
CN113106082B (zh) 动物粪便宏基因组来源的丙氨酸消旋酶及其制备和应用
KR100244066B1 (ko) D-N-카르바모일-α-아미노산의 제조법
US20120149071A1 (en) D-aminoacylase
US6902915B2 (en) Heat-stable D-aminoacylase
WO2022138969A1 (fr) Hydroxylase d'acide l-pipécolique mutante et procédé de production d'acide cis-5-hydroxy-l-pipécolique l'utilisant
JPH02234678A (ja) アミノ酸アミド加水分解酵素及びその使用
JP4627039B2 (ja) アミダーゼ活性を有するポリペプチド及びその遺伝子
JP4485734B2 (ja) ５置換ヒダントインラセマーゼ、これをコードするｄｎａ、組み換えｄｎａ、形質転換された細胞および光学活性アミノ酸の製造方法
JP2006055131A (ja) 新規なｄ−アミノアシラーゼおよびその遺伝子
JP5096911B2 (ja) ５−置換ヒダントインラセマーゼ、これをコードするｄｎａ、組換えｄｎａ、形質転換された細胞、および、光学活性ｎ−カルバミルアミノ酸または光学活性アミノ酸の製造方法
JP2009165417A (ja) 新規過酸化水素生成型ｎａｄｈオキシダーゼ及びそれをコードするｄｎａ
JP2008194037A (ja) 生体触媒による４−ハロ−３−ヒドロキシ酪酸エステルの光学分割法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11800912

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2012522670

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 11800912

Country of ref document: EP

Kind code of ref document: A1