WO2011122889A3 - miRNA의 발현 억제를 이용한 성체줄기세포의 노화 억제 방법 - Google Patents

miRNA의 발현 억제를 이용한 성체줄기세포의 노화 억제 방법 Download PDF

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WO2011122889A3
WO2011122889A3 PCT/KR2011/002249 KR2011002249W WO2011122889A3 WO 2011122889 A3 WO2011122889 A3 WO 2011122889A3 KR 2011002249 W KR2011002249 W KR 2011002249W WO 2011122889 A3 WO2011122889 A3 WO 2011122889A3
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adult stem
inhibiting
stem cells
senescence
cell
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WO2011122889A2 (ko
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강경선
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주식회사 강스템홀딩스
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
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    • C12N2501/065—Modulators of histone acetylation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/65—MicroRNA

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Abstract

본 발명은 성체줄기세포의 노화 억제 방법에 관한 것으로서, 더욱 상세하게는 성체줄기세포에서 발현하는 miRNA-23a, miRNA-26a 및 miRNA-30a로 구성된 군에서 선택되는 어느 하나의 발현을 특이적인 억제제를 이용하여 발현을 억제시킴으로써, high mobility group A2(HMGA2)의 발현을 증가시키고, p16 또는 p21의 발현을 감소시켜 성체줄기세포의 노화를 억제하는 방법에 관한 것이다. 본 발명에 따른 성체줄기세포의 노화 억제 방법은 성체줄기세포 배양시 계대배양을 시행할수록 세포의 노화가 빠른 속도로 진행되어 세포 분열이 급격히 감소하는 문제점을 해결할 수 있다. 이로써 분화능과 세포 재생 능력을 그대로 유지한 성체줄기세포를 오래 유지할 수 있어 세포 치료제 혹은 연구용으로 사용할 수 있는 세포를 더욱 많이 확보할 수 있다.
PCT/KR2011/002249 2010-03-31 2011-03-31 miRNA의 발현 억제를 이용한 성체줄기세포의 노화 억제 방법 WO2011122889A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2010-0029545 2010-03-31
KR20100029545 2010-03-31

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WO2011122889A2 WO2011122889A2 (ko) 2011-10-06
WO2011122889A3 true WO2011122889A3 (ko) 2012-03-08

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PCT/KR2011/002249 WO2011122889A2 (ko) 2010-03-31 2011-03-31 miRNA의 발현 억제를 이용한 성체줄기세포의 노화 억제 방법

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KR (1) KR101299733B1 (ko)
WO (1) WO2011122889A2 (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101678249B1 (ko) * 2012-07-11 2016-11-22 서울대학교산학협력단 ｍｉＲＮＡ-141의 억제를 이용한 세포의 노화 억제 방법
KR101418876B1 (ko) * 2012-10-22 2014-07-30 서울대학교병원 (분사무소) 피부성체 줄기세포의 분화 조절 방법
KR20140121317A (ko) 2013-04-06 2014-10-15 서울대학교산학협력단 비신경 세포로부터 유도 신경 줄기 세포의 생산 방법 및 이에 의하여 생산되는 유도 신경 줄기 세포
KR102076475B1 (ko) * 2018-08-13 2020-02-12 울산과학기술원 성체 신경 발생을 조절하는 dscr1의 용도
KR102367105B1 (ko) 2020-07-15 2022-02-24 차의과학대학교 산학협력단 신규한 miRNA smR-78 및 이의 용도
WO2023013970A1 (ko) 2021-08-05 2023-02-09 경북대학교 산학협력단 T 세포의 세포외 소포체 유래 miRNA를 유효성분으로 포함하는 조성물 및 이의 용도
KR20230022800A (ko) 2021-08-05 2023-02-16 경북대학교 산학협력단 활성화 T 세포의 세포외 소포체 유래 miRNA를 유효성분으로 포함하는 조성물 및 이의 용도
CN115820641B (zh) * 2022-10-31 2023-08-15 深圳丹伦基因科技有限公司 一种新型miRNA抑制物及其在体外缓解iMSC衰老中的应用

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KR101299733B1 (ko) 2013-08-28
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