WO2011040360A1 - シアリルラクトース素材の分離方法 - Google Patents
シアリルラクトース素材の分離方法 Download PDFInfo
- Publication number
- WO2011040360A1 WO2011040360A1 PCT/JP2010/066669 JP2010066669W WO2011040360A1 WO 2011040360 A1 WO2011040360 A1 WO 2011040360A1 JP 2010066669 W JP2010066669 W JP 2010066669W WO 2011040360 A1 WO2011040360 A1 WO 2011040360A1
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- WIPO (PCT)
- Prior art keywords
- membrane
- milk
- protein
- sialyl lactose
- kda
- Prior art date
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- OIZGSVFYNBZVIK-FHHHURIISA-N 3'-sialyllactose Chemical compound O1[C@@H]([C@H](O)[C@H](O)CO)[C@H](NC(=O)C)[C@@H](O)C[C@@]1(C(O)=O)O[C@@H]1[C@@H](O)[C@H](O[C@H]([C@H](O)CO)[C@H](O)[C@@H](O)C=O)O[C@H](CO)[C@@H]1O OIZGSVFYNBZVIK-FHHHURIISA-N 0.000 title claims abstract description 89
- 239000000463 material Substances 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000012528 membrane Substances 0.000 claims abstract description 97
- 235000013336 milk Nutrition 0.000 claims abstract description 47
- 239000008267 milk Substances 0.000 claims abstract description 47
- 210000004080 milk Anatomy 0.000 claims abstract description 47
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 31
- 102000004169 proteins and genes Human genes 0.000 claims description 79
- 108090000623 proteins and genes Proteins 0.000 claims description 79
- 238000010979 pH adjustment Methods 0.000 claims description 21
- 108010046377 Whey Proteins Proteins 0.000 claims description 18
- 102000007544 Whey Proteins Human genes 0.000 claims description 17
- 239000005862 Whey Substances 0.000 claims description 15
- 235000020183 skimmed milk Nutrition 0.000 claims description 12
- 235000013350 formula milk Nutrition 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 235000021067 refined food Nutrition 0.000 claims description 8
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 abstract description 19
- 239000008101 lactose Substances 0.000 abstract description 19
- 235000013305 food Nutrition 0.000 abstract description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract description 6
- 239000011707 mineral Substances 0.000 abstract description 6
- 239000003814 drug Substances 0.000 abstract description 3
- 235000018102 proteins Nutrition 0.000 description 69
- 239000000243 solution Substances 0.000 description 32
- 230000000903 blocking effect Effects 0.000 description 13
- 238000000926 separation method Methods 0.000 description 13
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 12
- 239000007858 starting material Substances 0.000 description 12
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 239000012141 concentrate Substances 0.000 description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
- 239000012466 permeate Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 235000013351 cheese Nutrition 0.000 description 6
- 238000011026 diafiltration Methods 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 230000005764 inhibitory process Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 235000010755 mineral Nutrition 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 4
- 239000003463 adsorbent Substances 0.000 description 4
- 239000003456 ion exchange resin Substances 0.000 description 4
- 229920003303 ion-exchange polymer Polymers 0.000 description 4
- 239000004310 lactic acid Substances 0.000 description 4
- 235000014655 lactic acid Nutrition 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 241000700605 Viruses Species 0.000 description 3
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 235000020256 human milk Nutrition 0.000 description 3
- 210000004251 human milk Anatomy 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 3
- 235000011121 sodium hydroxide Nutrition 0.000 description 3
- 235000021119 whey protein Nutrition 0.000 description 3
- 108010076119 Caseins Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 235000015155 buttermilk Nutrition 0.000 description 2
- 239000005018 casein Substances 0.000 description 2
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 2
- 235000021240 caseins Nutrition 0.000 description 2
- 238000013375 chromatographic separation Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000004128 high performance liquid chromatography Methods 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- -1 sialic acid compound Chemical class 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 208000031886 HIV Infections Diseases 0.000 description 1
- 208000037357 HIV infectious disease Diseases 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 239000003929 acidic solution Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 235000020248 camel milk Nutrition 0.000 description 1
- 108010067454 caseinomacropeptide Proteins 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 235000020247 cow milk Nutrition 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000013861 fat-free Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 235000020251 goat milk Nutrition 0.000 description 1
- 235000020252 horse milk Nutrition 0.000 description 1
- 208000033519 human immunodeficiency virus infectious disease Diseases 0.000 description 1
- 235000011167 hydrochloric acid Nutrition 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 208000018773 low birth weight Diseases 0.000 description 1
- 231100000533 low birth weight Toxicity 0.000 description 1
- 239000011812 mixed powder Substances 0.000 description 1
- 238000001728 nano-filtration Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 235000008476 powdered milk Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000020185 raw untreated milk Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 108010058314 rennet Proteins 0.000 description 1
- 229940108461 rennet Drugs 0.000 description 1
- 235000020254 sheep milk Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/20—Dietetic milk products not covered by groups A23C9/12 - A23C9/18
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/40—Complete food formulations for specific consumer groups or specific purposes, e.g. infant formula
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/16—Feed pretreatment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
- C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
- C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K5/00—Lactose
Definitions
- the present invention uses milk as a starting material, removes protein from the milk material, adjusts the pH of the protein removal solution obtained after the protein removal step, and adjusts the pH adjustment solution obtained after the pH adjustment step.
- the present invention relates to a method for separating a sialyl lactose material, characterized by having a step of concentrating the sialyl lactose with an ultrafiltration membrane (UF membrane). Moreover, it is related with the food-drinks etc. which mix
- UF membrane ultrafiltration membrane
- sialyl lactose combined with sialic acid and lactose has a protective activity against viruses and bacteria, and physiological activities such as lactic acid bacteria growth activity. For this reason, sialyl lactose has been used in infant formulas (see, for example, Non-Patent Document 1). It is also known to have an HIV infection / proliferation inhibitory effect, and is expected to be applied to foods for these purposes (for example, see Patent Document 1).
- Patent Document 2 In the method using a simulated moving bed chromatographic separation apparatus (SMB) (Patent Document 2), there are problems such as complicated operating conditions for simultaneously purifying sialic acid compounds having different separation factors, and a large amount Since preparation requires a large-scale apparatus, it is not suitable for mass production.
- SMB simulated moving bed chromatographic separation apparatus
- Patent Document 3 The method using an ion exchange resin (Patent Document 3) is difficult to manufacture on a large scale due to problems such as poor chemical resistance and physical durability of the resin used and environmental pollution due to recycled waste liquid.
- an ultrafiltration membrane is used for concentrating sialyl lactose from a solution from which protein has been removed, but this can ensure Flux at a lower pressure than when a nanofiltration membrane (NF membrane) is used. Yes, the energy required for operation can be reduced.
- an object of the present invention is to provide a method for easily and efficiently separating a sialyl lactose material from a milk material, a food or drink containing the obtained sialyl lactose material, and the like.
- the present invention is an invention having any of the following configurations.
- (1) The step of removing protein from the milk material, the step of adjusting the pH of the protein removal solution obtained after the protein removal step, and the pH adjustment solution obtained after the pH adjustment step are subjected to ultrafiltration membrane (UF membrane)
- a method for separating a sialyl lactose material comprising the step of (2) The method for separating a sialyl lactose material according to (1), wherein in the step of removing the protein, the protein is removed from the milk material using an ultrafiltration membrane (UF membrane).
- the protein is removed from the milk material using an ultrafiltration membrane (UF membrane) having a molecular weight cut off of 5 kDa to 16 kDa, and the pH adjustment obtained after the pH adjustment step in the concentration step.
- UF membrane ultrafiltration membrane
- UF membrane ultrafiltration membrane
- a sialyllactose material can be separated with high purity by performing a simple operation using a milk material as a starting material.
- Milk materials used as raw materials include skim milk obtained from mammals such as cow's milk, goat's milk, sheep milk, camel milk, horse milk, cheese whey, acid whey, rennet whey, butter milk and the like. Since these contain proteins and interfere with the efficiency of the membrane treatment in the next step, it is necessary to remove the proteins.
- Examples of the method for removing protein include membrane treatment, adsorption treatment, aggregation treatment, and the like, but are not particularly limited as long as the method can remove protein.
- an ultrafiltration membrane UF membrane
- a membrane with a molecular weight cut off of 5 kDa to 16 kDa it is possible to efficiently remove proteins, particularly whey proteins, and furthermore, by using a molecular weight cut off of 10 kDa, a sialyl lactose material can be obtained efficiently. .
- ultrafiltration membrane HFK131 (manufactured by KOCH Membrane System), PW (manufactured by GE Osmonics), or the like
- adsorbent can be used, and the protein can be removed from the milk material by bringing the adsorbent into contact with the milk material and adsorbing the protein to the adsorbent.
- QMA Spherosil (Pall) or S Spherosil (Pall) can be used as the ion exchange resin as the adsorbent.
- proteins can be aggregated by pH adjustment and / or heating, and the aggregated proteins can be removed by solid-liquid separation.
- pH pH 4.6
- casein occupying about 80% of the protein in milk can be aggregated.
- pH pH 3.5 to 5.5 and / or heating to 55 ° C. or higher
- about the amount of protein in milk Aggregates 20% whey protein.
- Acid or alkali can be used for pH adjustment, and specifically, for example, hydrochloric acid, lactic acid, caustic soda and the like can be used.
- As a solid-liquid separation method in milk sedimentation separation, pressing, etc. can be used.
- Separator MSD manufactured by GEA Westfalia Separator
- Clarifying Decanter CA GEA Westfalia Separator
- a protein removing solution from which at least about 80%, preferably almost all of the protein in the milk has been removed can be obtained.
- the protein concentrate produced as a by-product in the protein removal step contains a large amount of protein, and therefore can be used as a raw material for protein materials such as MPC and WPC.
- pH adjustment of the protein removing solution is very important.
- the main component of the solute contained in the protein removal solution is lactose. Therefore, what is important in fractionating sialyl lactose from the protein removal solution is the fraction of sialyl lactose and lactose.
- the inventors have found that the following properties are obtained. That is, the blocking rate of sialyl lactose on the ultrafiltration membrane depends on the pH of the protein removal solution, the blocking rate is high on the acidic side, and the blocking rate on the neutral side is low. The inhibition rate is also dependent on the pH of the protein removal solution.
- the rejection rate refers to the degree to which a specific component (for example, component A) in membrane separation is focused and the component is blocked without passing through the membrane, and the rejection rate is defined by the following equation.
- Inhibition rate of component A (%) (1- (Concentration of component A in permeate / Concentration of component A in concentrate)) x 100
- the blocking rate of sialyl lactose is high, the blocking rate of lactose is low, and the blocking rate of the two components is greatly different. By utilizing this difference in blocking rate, sialyl lactose and lactose can be fractionated, and sialyl lactose can be concentrated from the protein removal solution.
- the present inventors have been able to clarify specific pH regions where sialyl lactose and lactose can be efficiently fractionated for each milk material. It was. That is, when skim milk is used as the starting material, the pH is preferably 6.6 to 5.6, more preferably pH 6.3 to 5.6, and when whey is used, the pH is preferably 5.5 to 3.6. Is pH 5.0 to 3.6, particularly preferably pH 4.5 to 3.6.
- an acidic solution or an alkaline solution is used according to the pH after the above protein removal. Examples of the solution to be used include, but are not limited to, hydrochloric acid or sodium hydroxide solution.
- this pH adjusting solution is concentrated with an ultrafiltration membrane (UF membrane).
- a membrane having a fractional molecular weight of 600 Da to 3 kDa can be preferably used, and a membrane having a fractional molecular weight of 1 kDa can be more preferably used.
- Membralox 1 kDa Pall Exekia
- XT membrane Spher Filtration
- sialyl lactose can be fractionated.
- DF diafiltration
- UF membrane ultrafiltration membrane
- a certain amount of lactose from the ultrafiltration membrane (UF membrane) concentrate Mineral content can be removed and sialyl lactose concentration can be increased.
- sialyl lactose can be obtained with high purity. Therefore, the obtained sialyl lactose material has a high utility value as a food or a medicine.
- the obtained sialyl lactose material can be blended into processed foods, foods such as prepared milk powder, pharmaceuticals, feeds and the like.
- Processed foods include processed foods mainly composed of sugar, glucose, maltose, corn starch, dextrin, sugar alcohol, lactose, etc., and 0.1 to 90 g of sialyl lactose material can be blended per 100 g of product.
- the processed food can be in any state such as solid, semi-solid, and liquid, but can also be in tablet form.
- the reason why the lower limit of blending is 0.1 g or more per 100 g is because it is combined with the sialyl lactose content (as solid 100%) in human milk (breast milk). This is because an effective effect may not be obtained.
- Formulated milk includes infant formula, formula for low birth weight infants, follow-up milk, formula for infants with allergic diseases, and the like, and 0.1 to 30 g of sialyl lactose material can be blended per 100 g of product. This is because lactose is mainly used as the component content of the present sialyl lactose material, and it is difficult to mix more than 30 g of this sialyl lactose material per 100 g of product from the component composition of the above-mentioned formula milk powder. Also, the reason why the lower limit of blending is 0.1 g or more per 100 g is that it is combined with the sialyl lactose content (as 100% solids) in human milk (milk). This is because there are cases where a small or effective effect may not be obtained.
- Example 1 (Examination of protein removal method by membrane) The membrane pore size in the protein removal of the starting material was examined. Nonfat milk was used as the starting milk material. A protein removal solution was obtained using a UF membrane with a molecular weight cut off of 10 kDa (HFK131 (manufactured by KOCH Membrane System)) or a molecular weight cut off of 5 kDa (Membralox 5 kDa (manufactured by Pall Exekia)). The protein removal solution was adjusted to pH 6.3, 5.6, and 4.5 with 1N hydrochloric acid, and treated at 50 ° C.
- HFK131 manufactured by KOCH Membrane System
- 5 kDa manufactured by Pall Exekia
- sialyl lactose contained in the concentrated solution and permeate was measured by high performance liquid chromatography (HPLC).
- HPLC high performance liquid chromatography
- DX500 manufactured by DIONEX was used, and CarboPac PA1 was used as a column.
- the mobile phase was 60 mM sodium acetate / 100 mM sodium hydroxide aqueous solution, and the flow rate was 1 ml / min.
- the inhibition rate of sialyl lactose was calculated from the concentration of the obtained sialyl lactose.
- a 10 kDa ultrafiltration membrane (UF membrane) was used for protein removal.
- the sialyllactose inhibition rate at 1 kDa UF membrane was 100% at all pH 6.3 to 4.5 after adjustment, whereas when 5 kDa ultrafiltration membrane (UF membrane) was used for protein removal It was found that when the pH was adjusted to 5.6 and 6.3, the sialyllactose blocking rate at the 1 kDa UF membrane was 77.4% and 42.2%, and sialyllactose leaked into the permeate.
- Example 2 (Optimal pH when using cheese whey as starting material)
- the processing conditions when using cheese whey as a starting milk material were examined.
- the cheese whey was treated at 10 ° C. using a UF membrane (HFK131 (manufactured by KOCH Membrane System)) having a molecular weight cut off of 10 kDa to obtain a protein removing solution from which proteins were removed.
- the permeate protein remover was adjusted to pH with 1N hydrochloric acid as appropriate and subjected to total circulation treatment at 50 ° C. using a UF membrane with a molecular weight cut off of 1 kDa.
- the concentration of sialyl lactose and lactose in the obtained permeate was measured, and each blocking rate was evaluated.
- the concentration of sialyl lactose was the same as in Example 1, and the concentration of lactose was measured by a Brix meter (manufactured by Atago Co., Ltd.).
- the evaluation results are shown in Table 2.
- the treatment pH is adjusted to 5.5 to 3.6, especially 5.0 to 3.6, and the difference in the blocking rate between sialyl lactose and lactose increases, so sialyl lactose is most efficiently concentrated. it can.
- Example 3 (Optimal pH using skim milk as starting material) The processing conditions when skim milk was used as a starting material for milk materials were examined.
- the skim milk was treated at 10 ° C. using a UF membrane (HFK131 (manufactured by KOCH Membrane System)) having a molecular weight cut off of 10 kDa to obtain a protein removing solution from which proteins were removed.
- the protein removal solution was adjusted to pH appropriately with 1N hydrochloric acid, and subjected to total circulation treatment at 50 ° C. using a UF membrane with a molecular weight cut off of 1 kDa.
- the concentration of sialyl lactose and lactose in the obtained permeate was measured, and each blocking rate was evaluated.
- sialyl lactose concentration was evaluated in the same manner as in Example 1, and the lactose concentration was evaluated in the same manner as in Example 2.
- the evaluation results are shown in Table 3.
- sialyllactose can be most efficiently concentrated by setting the treatment pH to 6.6 to 5.6, particularly 6.3 to 5.6.
- Example 4 (Separation of sialyl lactose material when cheese whey is used as starting material) 610 kg of cheese whey was concentrated 5-fold at 10 ° C. using a UF membrane (PW3838-50D (manufactured by GE Osmonics)) having a molecular weight cut off of 10 kDa to obtain 488 kg of a protein removing solution from which protein was removed.
- a UF membrane PW3838-50D (manufactured by GE Osmonics)
- the protein removal solution was adjusted to pH 5.0 with 1N hydrochloric acid, concentrated 44 times at 50 ° C using a UF membrane (Membralox 1 kDa (manufactured by Pall Exekia)) with a molecular weight cut off of 1 kDa, and then 0.9 times diafiltration (DF ) Treatment to obtain a sialyl lactose concentrate.
- Table 4 shows the component composition of the sialyllactose-rich material that was freeze-dried. By the above treatment, a material having a sialyllactose content per solid of 0.65% was obtained.
- Example 5 (Separation of sialyl lactose material when skim milk is used as a starting material) 700 kg of raw milk was separated with a milk separator to obtain 605 kg of skim milk. After sterilization at 75 ° C., UF membrane (DESAL PW3838-30D (manufactured by GE Osmonics)) having a molecular weight cut off of 10 kDa was concentrated 4 times at 50 ° C. to obtain 450 kg of protein removal solution from which protein was removed. This protein removal solution was concentrated with a UF membrane (Membralox 1 kDa (manufactured by Pall Exekia)) having a molecular weight cut off of 1 kDa adjusted to pH 6.3.
- UF membrane DESAL PW3838-30D (manufactured by GE Osmonics)
- 1.1-fold diafiltration (DF) treatment was performed to obtain a sialyllactose-rich material.
- the component composition of the sialyl lactose material lyophilized is shown in Table 5.
- Example 6 According to the composition shown in Table 6, whey powder (manufactured by Snow Brand Milk Products Co., Ltd.), skim milk powder (manufactured by Snow Brand Milk Products Co., Ltd.), protein concentrated whey powder (manufactured by Snow Brand Milk Products Co., Ltd.), butter milk (manufactured by Snow Brand Milk Products Co., Ltd.), all Powdered milk (manufactured by Snow Brand Milk Products Co., Ltd.) is mixed and dissolved, and casein (manufactured by FONTERRA), whey protein concentrate (manufactured by FONTERRA), vitamins (manufactured by BASF), minerals (manufactured by Komatsuya Chemical Co., Ltd.) ), Sialyllactose material produced in Example 5, and other trace materials.
- infant formula which strengthened sialyl lactose was manufactured.
- the obtained infant formula has enhanced sialyllactose, and thus has physiological activity effects such as infection protection against viruses and bacteria, and lactic acid bacteria growth activity.
- Example 7 According to the formulation shown in Table 7, total sugar-glucose (manufactured by Sanei Saccharification Co., Ltd.), sucrose ester (manufactured by Mitsubishi Chemical Foods Co., Ltd.), crystalline cellulose (manufactured by Asahi Kasei Chemicals Co., Ltd.), and fragrance (manufactured by Takasago Fragrance Industries, Ltd.)
- the mixed powder obtained by mixing and adding the sialyl lactose material produced in Example 5 was directly compressed by applying a pressure of 1 to 3 t to obtain 1 g of a tablet containing sialyl lactose. Since the obtained tablets are reinforced with sialyl lactose, they have a physiologically active effect such as a protective action against viruses and bacteria, and a lactic acid bacteria growth activity.
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Abstract
Description
(1)乳素材からタンパク質を除去する工程と、前記タンパク質除去工程後に得られたタンパク質除去液をpH調整する工程と、前記pH調整工程後に得られたpH調整液を限外濾過膜(UF膜)で濃縮する工程を有することを特徴とするシアリルラクトース素材の分離方法。
(2)タンパク質を除去する工程において、限外濾過膜(UF膜)を用いて乳素材からタンパク質を除去することを特徴とする上記(1)記載のシアリルラクトース素材の分離方法。
(3)タンパク質を除去する工程において、分画分子量5kDa~16kDaの限外濾過膜(UF膜)を用いて乳素材からタンパク質を除去し、濃縮工程において、前記pH調整工程後に得られたpH調整液を分画分子量600Da~3kDaの限外濾過膜(UF膜)で濃縮することを特徴とする上記(1)又は(2)記載のシアリルラクトース素材の分離方法。
(4)前記乳素材として脱脂乳を使用するとともに、前記pH調整工程においてpHを6.6~5.6に調整することを特徴とする上記(1)~(3)のいずれかに記載のシアリルラクトース素材の分離方法。
(5)前記乳素材としてホエイを使用するとともに、前記pH調整工程においてpHを5.5~3.6に調整することを特徴とする上記(1)~(3)のいずれかに記載のシアリルラクトース素材の分離方法。
(6)乳素材からタンパク質を除去する工程と、前記タンパク質除去工程後に得られたタンパク質除去液をpH調整する工程と、前記pH調整工程後に得られたpH調整液を限外濾過膜(UF膜)で濃縮する工程により得られるシアリルラクトース素材。
(7)上記(6)に記載のシアリルラクトース素材を配合した加工食品。
(8)上記(6)に記載のシアリルラクトース素材を、製品100g当たり0.1~90g配合した加工食品。
(9)上記(6)に記載のシアリルラクトース素材を配合した調製粉乳。
(10)上記(6)に記載のシアリルラクトース素材を、製品100g当たり0.1~30g配合した調製粉乳。
膜処理においては、限外濾過膜(UF膜)を用いることができる。特に、分画分子量5kDa~16kDaの膜を用いることにより、効率よくタンパク質、特にホエイタンパク質を除去することができ、さらに、分画分子量10kDaを用いることにより効率よく、シアリルラクトース素材を得ることができる。具体的には、例えば、限外濾過膜(UF膜)としてはHFK131(KOCH Membrane System社製)やPW(GE Osmonics社製)等を使用することが可能である。
吸着処理においては、吸着剤を用いることができ、吸着剤と乳素材を接触させてタンパク質を吸着剤に吸着させることで乳素材からタンパク質を除去することができる。吸着剤としては、具体的には、例えば、イオン交換樹脂としてはQMA Spherosil(Pall社製)やS Spherosil(Pall社製)を使用することが可能である。
凝集処理においては、pH調整および/または加熱によりタンパク質を凝集させ、凝集したタンパク質は固液分離により除去することができる。pHを4.6に調整することで乳中のタンパク質の約8割を占めるカゼインを凝集させることができ、pHを3.5~5.5に調整および/または55℃以上に加熱することで乳中のタンパク質の約2割を占めるホエイタンパクを凝集させることができる。pH調整には酸やアルカリを用いることができ、具体的には、例えば塩酸、乳酸、苛性ソーダ等が使用可能である。乳中の固液分離方法としては、沈降分離、圧搾等を用いることができ、具体的には、例えば、沈降分離としてはSeparator MSD(GEA Westfalia Separator社製)を、圧搾としてはClarifying Decanter CA(GEA Westfalia Separator社製)を用いることができる。
これらの操作により、乳中のタンパク質の少なくとも約8割、望ましくはほぼ全てが除去されたタンパク質除去液を得ることができる。一方、タンパク質除去工程で副産物として生成するタンパク質濃縮液中にはタンパク質が多量に含まれていることから、MPCやWPC等のタンパク質素材の原料として用いることができる。
タンパク質除去液に含まれる溶質の主成分は乳糖である。よって、タンパク質除去液からシアリルラクトースを分画するにあたって重要となるのは、シアリルラクトースと乳糖の分画である。本発明者らは、タンパク質除去液を限外濾過膜で処理する際のシアリルラクトースと乳糖の挙動を注意深く検討した結果、以下の特性を有することを見出した。すなわち、シアリルラクトースの限外濾過膜での阻止率はタンパク質除去液のpHに依存し、酸性側で阻止率が高く、中性側で阻止率が低いことと、乳糖の限外濾過膜での阻止率もタンパク質除去液のpHに同様に依存することである。
ここで、阻止率とは膜分離においてある特定の成分(例えば成分A)に注目して、その成分が膜を透過せずに阻止される度合いのことで、阻止率は下式で定義される。
成分Aの阻止率(%)=
(1-(透過液中の成分Aの濃度 / 濃縮液中の成分Aの濃度))×100
また、特筆すべきは、ある特定のpH領域においては、シアリルラクトースの阻止率が高く、乳糖の阻止率が低くなり、二つの成分の阻止率に大きな差が生じることである。この阻止率の差を利用することで、シアリルラクトースと乳糖の分画が可能となり、タンパク質除去液からシアリルラクトースを濃縮することができるものである。更に、本発明者らは、乳素材から得た複数のタンパク質除去液について、鋭意検討した結果、乳素材それぞれについてシアリルラクトースと乳糖を効率よく分画できる特定のpH領域を明らかにすることができた。すなわち、出発原料に脱脂乳を用いた場合には好ましくはpH6.6~5.6に、より好ましくはpH6.3~5.6に、ホエイを用いた場合には好ましくはpH5.5~3.6に、より好ましくはpH5.0~3.6、特に好ましくはpH4.5~3.6である。
pH調整方法としては、上記のタンパク質除去後のpHに応じて、酸性溶液又はアルカリ性溶液を用いて行う。用いられる溶液としては、塩酸あるいは水酸化ナトリウム溶液が挙げられるが、これらに限定されるものではない。また、ミネラル分の増加を抑えるために、イオン交換樹脂処理によるpH調整を実施することも可能である。ただし、処理pHを高くしすぎるとリン酸カルシウムが析出し、次工程で膜閉塞が生じ、流量の低下が生じることから、上記の範囲内でpHを設定することが望ましい。
なお、シアリルラクトースを含む限外濾過膜(UF膜)濃縮液に、水を加えながら濃縮する透析濾過(DF)処理を施すと、限外濾過膜(UF膜)濃縮液から一定量の乳糖とミネラル分を除去することができ、シアリルラクトース濃度を高めることができる。
本発明では、乳糖やミネラル分との分画も可能なため、シアリルラクトースを高純度で得ることができる。よって、得られたシアリルラクトース素材は、食品や医薬品等として利用価値が高いものとなる。
加工食品としては、砂糖、ブドウ糖、麦芽糖、コーンスターチ、デキストリン、糖アルコール、乳糖等を主原料とする加工食品等が含まれ、製品100g当たり0.1~90gのシアリルラクトース素材を配合することができる。なお、加工食品は、固体、半固体、液体等いかなる状態とすることもできるが、錠剤状とすることもできる。配合下限が100g当り0.1g以上とするのは、人乳(母乳)中のシアリルラクトース含量(固形100%として)に併せているからであり、0.1g未満であると、配合する意義が少ない若しくは有効な効果が得られない場合があるからである。
調製粉乳としては、乳児用調製粉乳、低出生体重児用調製粉乳、フォローアップミルク、アレルギー疾患児用調製粉乳等が含まれ、製品100g当たり0.1~30gのシアリルラクトース素材を配合することができる。本シアリルラクトース素材の成分的含量としては乳糖が主であり、上記調製粉乳の成分組成上から本シアリルラクトース素材を製品100g当たり30gを超えて配合することは難しいからである。また、配合下限が100g当り0.1g以上とするのは、人乳(母乳)中のシアリルラクトース含量(固形100%として)に併せているからであり、0.1g未満であると、配合する意義が少ない若しくは有効な効果が得られない場合があるからである。
(膜によるタンパク質除去方法の検討)
出発原料のタンパク質除去における膜孔径について検討を行った。
出発原料の乳素材として脱脂乳を用いた。これを分画分子量10kDa(HFK131(KOCH Membrane System社製))または、分画分子量5kDa(Membralox 5kDa(Pall Exekia社製))のUF膜を用いてタンパク質除去液を得た。このタンパク質除去液を、1N塩酸を用いてpHを6.3、5.6、4.5に調整し、それぞれ分画分子量1kDaのUF膜(Membralox 1kDa(Pall Exekia社製))を用いて50℃で処理し、得られた濃縮液と透過液に含まれるシアリルラクトースの濃度を高性能液体クロマトグラフ(HPLC)にて測定した。HPLCシステムとしては、DIONEX社製のDX500を使用し、カラムはCarboPac PA1を用いた。移動相には60mM酢酸ナトリウム/100mM水酸化ナトリウム水溶液を用い、流量は1ml/分とした。
得られたシアリルラクトースの濃度から、シアリルラクトースの阻止率を算出した。
タンパク質除去に10kDaの限外濾過膜(UF膜)を用いた場合と5kDaの限外濾過膜(UF膜)を用いた場合を比較すると、タンパク質除去に10kDaの限外濾過膜(UF膜)を用いた場合は調整後pH6.3~4.5の全てにおいて1kDaUF膜でのシアリルラクトース阻止率が100%であったのに対し、タンパク質除去に5kDaの限外濾過膜(UF膜)を用いた場合は調整後pHを5.6と6.3とすると1kDaUF膜でのシアリルラクトース阻止率が77.4%と42.2%となって透過液へシアリルラクトースが漏れることがわかった。シアリルラクトースが透過液へ漏れるとシアリルラクトースの収率が低下して製造コストが高くなる。このことから、タンパク質除去に用いる限外濾過膜(UF膜)としては分画分子量10kDaがより望ましいことが分かる。
乳素材に含まれるタンパク質の95%以上は分子量が16kDaを上回ることから、タンパク質除去に用いる限外濾過膜(UF膜)の分画分子量は16kDa以下である必要がある。また、分画分子量5kDa未満であると、シアリルラクトースの阻止率が低くなってしまい除去効率が悪い。よって、タンパク質を除去する工程には、分画分子量5kDa~16kDaの限外濾過膜(UF膜)を用いるのがより好ましい。
(出発原料にチーズホエイを用いた場合の最適pH)
チーズホエイを出発原料の乳素材として用いた際の処理条件について検討を行った。
チーズホエイを分画分子量10kDaのUF膜(HFK131(KOCH Membrane System社製))を用いて10℃で処理をして、タンパク質を除去したタンパク質除去液を得た。この透過液タンパク質除去液を、1N塩酸を用いてpHを適宜調整し、分画分子量1kDaのUF膜を用いて50℃で全循環処理した。得られた透過液のシアリルラクトースと乳糖の濃度を測定し、それぞれの阻止率を評価した。シアリルラクトースの濃度は実施例1と同様に行い、乳糖の濃度はBrix計(アタゴ社製)の測定値とした。
評価結果を表2に示す。出発原料にホエイを用いた場合には、処理pHを5.5~3.6に、特に5.0~3.6にすることで、シアリルラクトースと乳糖の阻止率の差が大きくなることから、最も効率よくシアリルラクトースを濃縮できる。
(出発原料に脱脂乳を用いた最適pH)
脱脂乳を乳素材の出発原料として用いた際の処理条件について検討を行った。
脱脂乳を分画分子量10kDaのUF膜(HFK131(KOCH Membrane System社製))を用いて10℃で処理をして、タンパク質を除去したタンパク質除去液を得た。このタンパク質除去液を、1N塩酸を用いてpHを適宜調整し、分画分子量1kDaのUF膜を用いて50℃で全循環処理した。得られた透過液のシアリルラクトースと乳糖の濃度を測定し、それぞれの阻止率を評価した。シアリルラクトース濃度は実施例1と同様に、乳糖濃度は実施例2と同様に評価した。
評価結果を表3に示した。出発原料に脱脂乳を用いた場合には、処理pHを6.6~5.6に、特に6.3~5.6にすることで最も効率よくシアリルラクトースの濃縮が可能である。
(出発原料にチーズホエイを用いた場合のシアリルラクトース素材の分離)
チーズホエイ610kgを分画分子量10kDaのUF膜(PW3838-50D(GE Osmonics社製))を用いて10℃で5倍濃縮して、タンパク質を除去したタンパク質除去液488kg得た。このタンパク質除去液を1N塩酸でpH5.0に調整し、分画分子量1kDaのUF膜(Membralox 1kDa(Pall Exekia社製))を用いて50℃で44倍濃縮後、0.9倍の透析濾過(DF)処理を行い、シアリルラクトース濃縮液を得た。これを凍結乾燥したシアリルラクトース高含有素材の成分組成を表4に示す。上記の処理により、固形あたりのシアリルラクトース含量が0.65%の素材を得た。
(出発原料に脱脂乳を用いた場合のシアリルラクトース素材の分離)
生乳700kgをミルクセパレータにて分離し、脱脂乳を605kg得た。75℃達温殺菌後、分画分子量10kDaのUF膜(DESAL PW3838-30D(GE Osmonics社製))を用いて50℃で4倍濃縮し、タンパク質除去したタンパク質除去液を450kg得た。このタンパク質除去液をpH6.3に調整した、分画分子量1kDaのUF膜(Membralox 1kDa(Pall Exekia社製))で、濃縮した。36倍濃縮後、1.1倍の透析濾過(DF)処理を行い、シアリルラクトース高含有素材を得た。これを凍結乾燥したシアリルラクトース素材の成分組成を表5に示す。上記の処理により、固形あたりのシアリルラクトース含量が0.95%の素材を得ることができた。
表6に示す配合により、ホエイパウダー(雪印乳業株式会社製)、脱脂粉乳(雪印乳業株式会社製)、タンパク質濃縮ホエイパウダー(雪印乳業株式会社製)、バターミルク(雪印乳業株式会社製)、全粉乳(雪印乳業株式会社製)を混合溶解し、これにカゼイン(FONTERRA社製)、乳清タンパク質濃縮物(FONTERRA社製)、ビタミン類(BASF社製)、ミネラル類(小松屋化学株式会社製)を配合し、さらに実施例5で製造したシアリルラクトース素材を配合し、その他微量素材を配合した。そして油脂混合物(植田製油株式会社製)を添加・混合・殺菌し、噴霧乾燥した後、シアリルラクトースを強化した乳児用調製粉乳を製造した。
得られた乳児用調製粉乳は、シアリルラクトースが強化されているため、ウイルスや細菌に対する感染防御作用、乳酸菌増殖活性のような生理活性効果を有する。
表7に示す配合により、全糖ぶどう糖(サンエイ糖化株式会社製)、ショ糖エステル(三菱化学フーズ株式会社製)、結晶セルロース(旭化成ケミカルズ株式会社製)、香料(高砂香料工業株式会社製)を混合し、これに実施例5で製造したシアリルラクトース素材を添加した混合粉末を1~3tの圧力を掛けて直接打錠し、シアリルラクトースを含有した1gの錠剤を得た。
得られた錠剤は、シアリルラクトースが強化されているため、ウイルスや細菌に対する感染防御作用、乳酸菌増殖活性のような生理活性効果を有する。
Claims (10)
- 乳素材からタンパク質を除去する工程と、前記タンパク質除去工程後に得られたタンパク質除去液をpH調整する工程と、前記pH調整工程後に得られたpH調整液を限外濾過膜(UF膜)で濃縮する工程を有することを特徴とするシアリルラクトース素材の分離方法。
- タンパク質を除去する工程において、限外濾過膜(UF膜)を用いて乳素材からタンパク質を除去することを特徴とする請求項1記載のシアリルラクトース素材の分離方法。
- タンパク質を除去する工程において、分画分子量5kDa~16kDaの限外濾過膜(UF膜)を用いて乳素材からタンパク質を除去し、濃縮する工程において、前記pH調整工程後に得られたpH調整液を分画分子量600Da~3kDaの限外濾過膜(UF膜)で濃縮することを特徴とする請求項1又は2記載のシアリルラクトース素材の分離方法。
- 前記乳素材として脱脂乳を使用するとともに、前記pH調整工程においてpHを6.6~5.6に調整することを特徴とする請求項1~3のいずれかに記載のシアリルラクトース素材の分離方法。
- 前記乳素材としてホエイを使用するとともに、前記pH調整工程においてpHを5.5~3.6に調整することを特徴とする請求項1~3のいずれかに記載のシアリルラクトース素材の分離方法。
- 乳素材からタンパク質を除去する工程と、前記タンパク質除去工程後に得られたタンパク質除去液をpH調整する工程と、前記pH調整工程後に得られたpH調整液を限外濾過膜(UF膜)で濃縮する工程により得られるシアリルラクトース素材。
- 請求項6に記載のシアリルラクトース素材を配合した加工食品。
- 請求項6に記載のシアリルラクトース素材を、製品100g当たり0.1~90g配合した加工食品。
- 請求項6に記載のシアリルラクトース素材を配合した調製粉乳。
- 請求項6に記載のシアリルラクトース素材を、製品100g当たり0.1~30g配合した調製粉乳。
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EP10820475.1A EP2484686A4 (en) | 2009-09-29 | 2010-09-27 | METHOD FOR SEPARATING A SIALYL LACTOSE MATERIAL |
US13/498,604 US20120245119A1 (en) | 2009-09-29 | 2010-09-27 | Method for separating sialyllactose material |
NZ598634A NZ598634A (en) | 2009-09-29 | 2010-09-27 | Method for separating sialyllactose material |
AU2010301761A AU2010301761A1 (en) | 2009-09-29 | 2010-09-27 | Method for separating sialyllactose material |
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US10729707B2 (en) | 2016-07-28 | 2020-08-04 | Fonterra Co-Operative Group Limited | Dairy product and process |
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CN103804433A (zh) * | 2012-11-06 | 2014-05-21 | 重庆药友制药有限责任公司 | 乳糖的精制方法 |
WO2019003133A1 (en) | 2017-06-30 | 2019-01-03 | Glycom A/S | PURIFICATION OF OLIGOSACCHARIDES |
MX2020012855A (es) * | 2018-06-01 | 2021-02-17 | Jennewein Biotechnologie Gmbh | Proceso sencillo para la purificacion de una sialilactosa. |
JP7338978B2 (ja) * | 2019-02-01 | 2023-09-05 | 雪印メグミルク株式会社 | 乳幼児用栄養組成物 |
EP4077702A4 (en) * | 2019-12-19 | 2023-11-01 | Glycom A/S | SEPARATION OF SIALYLATED OLIGOSACCHARIDES FROM A FERMENTATION BROTH |
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- 2010-09-27 EP EP10820475.1A patent/EP2484686A4/en not_active Withdrawn
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US10729707B2 (en) | 2016-07-28 | 2020-08-04 | Fonterra Co-Operative Group Limited | Dairy product and process |
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