WO2011006993A1 - Utilisation de substances naturelles en tant qu'additifs alimentaires pour animaux aquatiques - Google Patents

Utilisation de substances naturelles en tant qu'additifs alimentaires pour animaux aquatiques Download PDF

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Publication number
WO2011006993A1
WO2011006993A1 PCT/EP2010/060325 EP2010060325W WO2011006993A1 WO 2011006993 A1 WO2011006993 A1 WO 2011006993A1 EP 2010060325 W EP2010060325 W EP 2010060325W WO 2011006993 A1 WO2011006993 A1 WO 2011006993A1
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WIPO (PCT)
Prior art keywords
alpha
feed
pinene
cinnamaldehyde
cresol
Prior art date
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PCT/EP2010/060325
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English (en)
Inventor
Marco Frehner
Viviane Verlhac-Trichet
Philippe Narbel
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Dsm Ip Assets B.V.
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Filing date
Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Priority to CN201080032387.9A priority Critical patent/CN102469808B/zh
Priority to EP10734483A priority patent/EP2453758A1/fr
Priority to CA2767873A priority patent/CA2767873C/fr
Priority to JP2012520052A priority patent/JP5881175B2/ja
Priority to AU2010272494A priority patent/AU2010272494B2/en
Publication of WO2011006993A1 publication Critical patent/WO2011006993A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics

Definitions

  • the present invention relates to the use of natural active substances selected from the group consisting of alpha-pinene, alpha-terpineol, cinnamaldehyde, di- hydroeugenol, eugenol, meta-cresol and terpinolene in the manufacture of feed for aquatic animals including fish and shrimp, especially for cold water fish as for example salmon, bream, bass and for warm water fish as for example carp, tilapia, catfish.
  • this invention relates to the use of at least two substances as defined above for the improvement of the feed conversion ratio and/or daily weight gain in fish, for reducing mortality by regulating the micro flora of the gut and/or by protecting the animal against infections caused by pathogenic microorganisms.
  • the present invention relates to a novel fish feed composition
  • a novel fish feed composition comprising as active ingredients at least two, preferably at least three or four active compound(s) selected from the group consisting of alpha-pinene, alpha-terpineol, cinnamaldehyde, dihydroeugenol, eugenol, meta-cresol and terpinolene.
  • feed or feed composition means any compound, preparation, mixture, or composition suitable for, or intended for intake by an animal.
  • Turnover rate is deter- mined by how fast the fish grow to a harvestable size. As an example, it takes from 12 to 18 months to raise Atlantic salmon from smolt (the physiological stage when the Atlantic salmon can first be transferred from fresh water to sea water) to harvestable size. A fast turnover has several positive results. First, it helps cash - -
  • patent WO2004/091307 describes the use of polyphenols, and other natural actives in feed to increase survival rate of Artemia after hatching.
  • WO2004/091307 is however silent with regard to the selection of compounds to be used.
  • the application of polyphenols to reduce mortality is in the disclosed case above only useful at time of hatching. It therefore remains a need in aquaculture to prevent the development of diseases, thereby reducing mortality by any prophylactic means including antimicrobial activity at the gut level.
  • the inventors of the present application surprisingly found that substances as de- fined above have a great potential for use in fish feed, e.g. for improving the feed conversion ratio (FCR) and/or weight gain and/or for the modulation of the gut flora. Further, the inventors surprisingly found that the substances, which are hereinafter also referred to as compounds, have also antimicrobial activity result- - -
  • the invention relates to methods for using at least two active compounds selected from the group consisting of alpha- pinene, alpha-terpineol, cinnamaldehyde, dihydroeugenol, eugenol, meta-cresol and terpinolene in fish feed for improving the Feed Conversion Ratio (FCR) and/or weight gain and/or for reducing mortality by modulation of the gut micro- flora and/or by preventing diseases caused by pathogenic microorganisms.
  • FCR Feed Conversion Ratio
  • selected compounds of the invention eg.: cinnamaldehyde, dihydroeugenol, eugenol, and meta-cresol exhibit excellent effects inhibiting the growth of Yersinia ruckeri, a pathogenic microorganism which causes Enteric Redmouth (ERM), a disease found especially in salmonids and causing high mortality.
  • ERM Enteric Redmouth
  • alpha-pinene and/or alpha-terpineol and/or cinnamaldehyde and/or dihydroeugenol and/or eugenol and/or meta-cresol and/or terpinolene is/are used to improve animal feed digestibility and/or maintain animal health by supporting immune system function.
  • the invention relates to methods for using at least three active compounds selected from the group consisting of alpha-pinene, alpha- terpineol, cinnamaldehyde, dihydroeugenol, eugenol, meta-cresol and terpinolene in fish feed for improving the Feed Conversion Ratio (FCR) and/or weight gain and/or for reducing mortality by modulation of the gut microflora and/or by preventing diseases caused by pathogenic microorganisms.
  • FCR Feed Conversion Ratio
  • alpha-pinene and cinnamaldehyde are used to im- prove animal feed digestibility and/or maintain animal health by supporting immune system function.
  • the FCR may be determined on the basis of a fish growth trial comprising a first treatment in which a mixture of at least two compounds according to the invention is added to the animal feed in a suitable concentration per kg feed, and a second treatment (control) with no addition of the compound(s) to the animal feed.
  • an improved FCR is lower than the control FCR.
  • the FCR is improved (i.e., reduced) as compared to the control by at least 1.0 %, preferably at least 1.5 %, 1.6 %, 1.7 %, 1.8 %, 1.9 %, 2.0 %, 2.1 %, 2.2 %, 2.3 %, 2.4 %, or at least 2.5 %.
  • gut designates the gastrointestinal or digestive tract (also referred to as the alimentary canal) and it refers to the system of organs within multi-cellular animals which takes in food, digests it to extract energy and nutrients, and expels the remaining waste.
  • gut “microflora” refers to the natural microbial cultures residing in the gut and maintaining health by aiding in proper digestion.
  • module as used herein in connection with the gut microflora generally means to change, manipulate, alter, or adjust the function or status thereof in a healthy and normally functioning animal, i.e. a non-therapeutic use.
  • supporting immune system function refers to the immune stimulation effect obtained by the compounds.
  • the term "mortality" as used herein refers to the ratio of life animals at the end of the growth phase versus the number of animals originally included into the pond. It may be determined on the basis of a fish challenge trial comprising two groups of fish challenged by a particular fish pathogen with the aim to provoke a mortality of 40 to 80 % of the animals in the untreated group. However, in the challenge group fed with a suitable concentration per Kg of feed of a mixture of at least two compounds according to the invention, the mortality is reduced compared to the - -
  • untreated group by at least 5 %, preferably at least, 10 %, 15 %, 20 %, 25 %, 30 %, 35 %, 40 %, 45 %, or at least 50 %.
  • the inventors of the present application surprisingly found that the compounds according to the invention and mixtures thereof are effective against a number pathogenic microorganisms of cold and warm water fish.
  • Alpha-pinene, alpha-terpineol, cinnamaldehyde, dihydroeugenol, meta-cresol and terpinolene were shown to exhibit inhibitory effect against Vibrio anguillarum, a shrimp pathogen causing vibriosis.
  • Alpha-pinene, cinnamaldehyde, dihydroeugenol, eugenol, limonene, and meta- cresol were shown to exhibit inhibitory effect against Aeromonas salmonicida which is the pathogen causing a disease known as furunculosis.
  • Alpha-pinene, alpha-terpineol, cinnamaldehyde, dihydroeugenol, eugenol, meta- cresol and terpinolene were shown to exhibit inhibitory effect against Edwardsellia tarda causing systemic infection in fish.
  • Alpha-pinene, alpha-terpineol, cinnamaldehyde, dihydroeugenol, eugenol, and terpinolene were shown to exhibit inhibitory effect against Lactococcus garvieae which is the etiological agent of Latococcosis, an emergent disease which affects many fish species and causes important economic losses both in marine and freshwater aquaculture when water temperature increases over 16 0 C in summer months.
  • Cinnamaldehyde, dihydroeugenol, eugenol, and meta-cresol exhibit excellent inhibitory effects on the growth of Yersinia ruckeri, a pathogenic microorganism which causes Enteric Redmouth (ERM), a disease found especially in salmonids. Cinnamaldehyde and meta-cresol were shown to exhibit inhibitory effect against:
  • Aeromonas hydrophila causing ulcers and hemorrhagic septicaemia. This pathogen is very resistant to conventional simple antimicrobials like chlorine.
  • Photobacte ⁇ um damselae formerly Pasteurella piscicida a pathogen causing high losses in the culture industry of economically important marine fishes such as seriola and red grouper in Japan and striped bass and white perch in the United States.
  • the present invention provides a fish feed composition
  • a fish feed composition comprising at least two active compound selected from the group consisting of alpha- pinene (CAS 99-86-5), alpha-terpineol (CAS 98-55-5), cinnamaldehyde (CAS 14371 -10-9 / 104-55-2), dihydroeugenol (CAS 2785-87-7), eugenol (CAS 97-53- 0), meta-cresol (CAS 108-39-4) and terpinolene (CAS 554-61 -0).
  • the compounds according to the invention are commercially available or can easily be prepared by a skilled person using processes and methods well-known in the prior art.
  • the compounds of the invention can be used alone or in mixtures thereof, in the form of natural available extracts or extract-mixtures or in the form of a natural substance. - -
  • extract as used herein includes compositions obtained by solvent extraction (which are also known as “extracted oils”), steam distillation (which are also known as “essential oils”) or other methods known to the skilled person.
  • Suitable extraction solvents include alcohols such as ethanol.
  • natural is in this context understood a substance which consists of compounds occurring in nature and obtained from natural products or through synthesis.
  • active compound(s) or natural substance or extract further ingredients may be added in minor amounts.
  • examples of such ingredients are: capsaicin, tannin, piperin, trimethylamine, 3,4,xylenol, furfuryl alcohol and mixtures thereof.
  • cinnamal- dehyde and/or meta-cresol are used as a major component of the mixture.
  • the mixture contains 10-90 % by weight of meta-cresol and/or cinnamalde- hyde, 1 -50 % by weight of alpha-pinene, 1 -50 % by weight of dihydro-eugenol, wherein the amounts being calculated on the total amount of said components.
  • the total amount of these active ingredients may vary within wide limits but is fi- nally used in the fish feed from 10 to 5000 ppm, preferably between 100 and 1000 ppm, calculated on the dry weight of the fish feed.
  • the most preferred mixture of at least two compounds as specified above comprises, cinnamaldehyde and alpha-pinene as a major component of the mixture.
  • the mixture contains 30-70 % by weight of alpha-pinene, 30-70 % by weight cinnamaldehyde, 1 -20 % by weight of metacresol, 1 -20 % by weight of dihydro-eugenol, wherein the amounts being calculated on the total amount of said components.
  • the total amount of these active ingredients may vary within wide limits but is finally used in the fish feed from 10 to 5000 ppm, preferably between 100 and 1000 ppm, calculated on the dry weight of the fish feed.
  • All compounds defined herein above may be used in combination with an emulsifying surfactant.
  • agent can be selected advantageously from those of a rather hydrophilic nature, for example among polyglycerol esters of fatty acids such as estehfied ricinoleic acid or propylene glycol esters of fatty acids, saccharo-esters or saccharo- glycerides, polyethylene glycol, lecithins etc.
  • polyglycerol esters of fatty acids such as estehfied ricinoleic acid or propylene glycol esters of fatty acids
  • saccharo-esters or saccharo- glycerides polyethylene glycol, lecithins etc.
  • a mixture of active compounds may contain 20 % by weight of cinnamaldehyde, 20 % by weight of meta-cresol, 20 % by weight of dihydro-eugenol, 20 % by weight of alpha-pinene, 3 % by weight of trimethylamine, 1.8 % by weight of piperin and 4 % by weight of furfuryl alcohol.
  • a mixture of active compounds contains 40 to 60 wt.-% alpha-pinene, 40 to 60 wt.-% cinnamaldehyde, and may further contain 1 to 5 wt.-% trimethylamine, 1 to 5 % piperin, and 3 to 8 wt.-% furfuryl alcohol.
  • the incorporation of the fish feed composition containing the active compound(s) into the fish feed may be performed as described in example 1.
  • the mixture of active compounds is then prepared directly as an oil which is then mixed with the oil sprayed onto the feed pellets as described in example 1.
  • the incorporation of the fish feed composition containing the active compound(s) into the fish feed may alternatively be carried out by preparing a premix of the active ingredients and other suitable additives.
  • a premix may comprise 2-10 % by weight of the active mixture or natural substance or extract, 0-40 % by weight of other conventional additives, such as flavorings, and 50-98 % by weight of any conventional absorbing support.
  • the support may contain, for example, 40-50 % by weight of wood fibers, 8-10 % by weight of stearin, 4-5 % by weight of curcuma powder, 4-5 % by weight of rosemary powder, 22-28 % by weight of limestone, 1 -3 % by weight of a gum, such as gum Arabic, 5-50 % by weight of sugar and/or starch and 5-15 % by weight of water.
  • a gum such as gum Arabic
  • This premix is then mixed with vitamins, as for example vitamin C, mineral salts and other feed additive ingredients, as for example yeast extracts containing nucleotides, glucan and other gut microflora modulators and then finally added to the feed in such quantities that the feed will contain 10-5000 ppm, preferably 100- 1000 ppm or 100-500 ppm of the active ingredients according to the invention.
  • the composition of the present invention will be preferably used together with yeast extract containing nucleotides, and glucan.
  • feed-additive ingredients are coloring agents, e.g. carotenoids such as beta-carotene, astaxanthin, and lutein; aroma compounds; stabilisers; antimicrobial peptides; polyunsaturated fatty acids; and/or at least one enzyme selected from amongst phytase (EC 3.1.3.8 or 3.1.3.26); xylanase (EC 3.2.1.8); galactanase (EC 3.2.1.89); alpha-galactosidase (EC 3.2.1.22); protease (EC 3.4.), phospholipase A1 (EC 3.1.1.32); phospholipase A2 (EC 3.1.1.4);
  • lysophospholipase (EC 3.1.1.5); phospholipase C (EC 3.1.4.3); phospholipase D
  • amylase such as, for example, alpha-amylase (EC 3.2.1.1 ); and/or beta-glucanase (EC 3.2.1.4 or EC 3.2.1.6).
  • polyunsaturated fatty acids are C18, C20 and C22 polyunsaturated fatty acids, such as arachidonic acid, docosohexaenoic acid, eicosapentaenoic acid and gamma-linoleic acid.
  • the fish feed as described herein has a proximate composition of 20-60 wt.-% protein, and 1 -45 wt.-% moisture and lipid.
  • the fish feed comprises one or more of sources of:
  • - protein, carbohydrate and lipid for example, fish meal, fish oil, blood meal, feather meal, poultry meal, chicken meal and/or other types of meal produced from other slaughterhouse waste
  • - vegetable meal e.g. soya meal, lupin meal, pea meal, bean meal, rape meal and/or sunflower meal
  • soya meal e.g. soya meal, lupin meal, pea meal, bean meal, rape meal and/or sunflower meal
  • - gluten e.g. wheat gluten or corn gluten
  • fish feed as used herein includes a fish feed composition according to the invention and components as described above.
  • fish feed includes fish meal as a component.
  • fish feed is in the form of flakes or pellets, for example extruded pellets.
  • the invention in a third aspect, relates to a feed composition for aquatic animals and to the use of this composition for feeding fish.
  • the feed is particularly suitable for feeding salmonids, including Atlantic salmon (Salmo salar), other salmon species and trout, and non-salmonids such as cod, sea bass, sea bream and eel.
  • salmonids including Atlantic salmon (Salmo salar), other salmon species and trout, and non-salmonids such as cod, sea bass, sea bream and eel.
  • it can be fed to all types of fish, for example turbot, halibut, yellow tail and tuna.
  • the main raw materials are ground and mixed. Microingredients are then added to the mixer and the homogenous mix is conditioned by adding water and steam to the mass in a preconditioner. This starts a cooking process in the starch fraction (the binding component).
  • the mass is fed into a pellet mill. The mass is forced through the mill's die and the strings are broken into pellets on the outside of the die. The moisture content is low and drying of the feed is not necessary.
  • Additional oil including a fish feed composition according to the present invention is then sprayed onto the surface of pellets, but as the pellets are rather compact, the total lipid content rarely exceeds 24 %.
  • the added oil may be fish oil or vegetable oils, for example rape seed oil or soy oil, or a mixture of vegetable oils or a mixture of fish oil and vegetable oils.
  • the pellets are cooled in a cooler and bagged.
  • the final pressed fish feed contains 10 to 5000 ppm of the composition as described in the invention.
  • the main raw materials are ground and mixed.
  • Micro ingredients incl. a fish feed composition according to the invention are added to the mixer.
  • the homogenous mix is conditioned by adding water and steam to the mass in a preconditioner. Additional oil may also be added to the mass at this stage. This starts a cooking process in the starch fraction (the binding component).
  • the mass is fed into an extruder.
  • the extruder may be of the single screw or the twin-screw type. Due to the rotational movement of the mass in the extruder, the mass is further mixed. Additional oil, water and steam may be added to the mass in the extruder. At the end of the extruder, the mass has a temperature above 100 0 C and a pressure above ambient pressure.
  • the mass is forced through the openings in the extruder's die plate. Due to the relief in temperature and pressure, some of the moisture will evaporate immediately (flash off) and the extruded mass becomes porous.
  • the strings are cut into pellets by a rotating knife. The water content is rather high (18-28 %) and the pellets are therefore immediately dried to approximately 10 % water content in a dryer.
  • oil may be added to the feed by spraying oil onto the surface of the feed, or by dipping the feed in oil. It is advantageous to add the oil to the feed in a closed vessel where the air pressure is below ambient (vacuum coating) so that the porous feed pellets absorb more oil. Feed containing more than 40 % lipid may be produced this way. After the coater, the feed is cooled and bagged. Oil may be added at several places in the process as explained above, and may - -
  • fish oil or vegetable oils by example rape seed oil or soy oil, or a mixture of vegetable oils or a mixture of fish oil and vegetable oils.
  • the dominant protein source in dry feed for fish has been fish meal of different qualities.
  • Other animal protein sources are also used for dry fish feed.
  • chicken meal typically cheaper than fish meal and fish oil.
  • vegetable protein such as wheat gluten, maize (corn) gluten, soya protein, lupin meal, pea meal, bean meal, rape meal, sunflower meal and rice flour.
  • Example 3 Evaluation of the effect of the active compounds according to the invention on survival of Yersinia ruckeri, Vibrio anguillarum, Vibrio sal- monicida, Aeromonas salmonicida, Aeromonas hydrophila, Edwardsellia tarda, Photobacterium damselae, Lactococcus garvieae, Streptococcus iniae. - -
  • composition of the invention The antimicrobial activity of the composition of the invention towards Yersinia ruckeri, Vibrio anguillarum, Vibrio salmonicida, Aeromonas salmonicida, Aeromo- nas hydrophila, Edwardsellia tarda, Photobacterium damselae, Lactococcus gar- vieae, and Streptococcus iniae were determined in vitro.
  • Concentration of test substances The in vitro dose range was estimated considering a probable dietary concentration of at least 1000 ppm and a daily feeding rate of 2 %. The potential concentration in the gut was established at maximum 0.1 ⁇ l/100 ⁇ l. A serial 2 dilution was then tested leading to final concentrations of the substance of 0.85 ⁇ g/ml; 0.42 ⁇ g/ml and 0.21 ⁇ g/ml when adjusted to average essential oil density. - -
  • Suitable concentration of bacteria for test A concentration of 1.5 % of 0.5 McFarland value resulted in a clearly visible spot covering 2/3 of a round bottom well. This concentration was therefore considered as suitable for the tests.
  • Table 1 Effect of different substances on growth of Yersinia ruckeri, Vibrio anguil- larum, Vibrio salmonicida, Aeromonas salmonicida, Aeromonas hydrophila, Ed- wardsellia tarda, Photobacterium damselae, Lactococcus garvieae, and Streptococcus iniae after 48 hours incubation at 22 0 C. Values represent means of 5 individual triplicates, nd: not determined, 3: no effect, 0: maximum growth inhibition. - -
  • Example 4 Evaluation of the effect of the active compounds according to the invention on survival of Vibrio anguillarum, and Aeromonas salmonicida.
  • Example 5 Effect of a combination of essential oils comprising alpha- pinene, cinnamaldehyde, dihydroeugenol and meta-cresol on the mortality of rainbow trout challenged with Aeromonas salmonicida.
  • a challenge experiment with Aeromonas salmonicida has been set up with juvenile rainbow trout in order to test the effect of three doses of a combination of four essential oils supplemented to the feed.
  • Diets and feeding One basal diet for juvenile rainbow trout was produced in 4 mm diameter. From these pellets, crumbles adapted to the size of the fish were produced and ca 5-kg batch of feed were produced by coating the essential oil combination at the different doses mixed with the oil onto the crumbles. Coating was performed using a peristaltic pump and a concrete mixer to insure homogenous distribution of the products.
  • the four experimental diets were produced:
  • the essential oil combination was the following one: alpha-pinene, cinnamalde- hyde, dihydroeugenol and meta-cresol at equivalent doses.
  • the experimental diets were fed for four weeks prior to challenge and after challenge until the end of the experiment. Fish weight was recorded at the start of experimental feeding, before challenge and at the end of the experiment on the remaining fish. - -
  • Challenge experiment The challenge dose was determined in a preliminary experiment, on the same fish population and under the same conditions. After a period of 4 weeks of experimental feeding, 25 fish were randomly distributed into four 38 I glass aquaria. Fish from each experimental tank were then anaesthetized and challenged by i.p. injection of Aeromonas salmonicida at a dose of 1.2 * 102 cfu/fish determined by spectrophotometry. The daily mortality was then recorded over a period of three weeks. First dead fish from each glass aquarium were subjected to a bacteriological investigation to confirm the bacteriological aetiology causing death. At the end of the experiment, all surviving fish were assessed for external signs of infection and in addition from 6 fish per diet group a necropsy and bacteriology were performed.
  • Example 6 Effect of an essential oil combination comprising alpha-pinene and cinnamaldehyde on the mortality of rainbow trout challenged with Aeromonas salmonicida.
  • a challenge experiment with Aeromonas salmonicida has been set up with juvenile rainbow trout in order to test the effect of alpha-pinene and cinnamaldehyde supplemented to the feed.
  • the experimental diets were fed for four weeks prior to challenge and after challenge until the end of the experiment.
  • Challenge experiment The challenge dose was determined in a preliminary experiment, on the same fish population and under the same conditions. After a period of 4 weeks of experimental feeding, 25 fish were randomly distributed into four 38 I glass aquaria. Fish from each experimental tank were then anaesthetized and challenged by i.p. injection of Aeromonas salmonicida at a dose of 5 * 10 3 cfu/fish determined by spectrophotometry. The daily mortality was then recorded over a period of three weeks. First dead fish from each glass aquarium were sub- - - -

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Abstract

La présente invention concerne l'utilisation de substances naturelles actives, choisies dans le groupe constitué de l'α-pinène, de l'α-terpinéol, du cinnamaldéhyde, du dihydroeugénol, de l'eugénol, du métacrésol et du terpinolène, dans le cadre de la fabrication de compositions alimentaires destinées aux animaux aquatiques, en particulier aux poissons d'eau froide tels que le saumon, la brème, la perche, mais aussi aux poissons d'eau chaude comme la carpe, le tilapia et le poisson-chat. La présente invention concerne, plus précisément, l'utilisation d'une substance telle que définie ci-dessus, en vue de l'amélioration du coefficient de transformation des aliments et/ou du gain pondéral quotidien chez le poisson, en vue de la régulation de la microflore intestinale et en vue de la protection de l'animal contre les infections provoquées par des microorganismes pathogènes.
PCT/EP2010/060325 2009-07-17 2010-07-16 Utilisation de substances naturelles en tant qu'additifs alimentaires pour animaux aquatiques WO2011006993A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CN201080032387.9A CN102469808B (zh) 2009-07-17 2010-07-16 天然物质作为用于水生动物的饲料添加剂的用途
EP10734483A EP2453758A1 (fr) 2009-07-17 2010-07-16 Utilisation de substances naturelles en tant qu'additifs alimentaires pour animaux aquatiques
CA2767873A CA2767873C (fr) 2009-07-17 2010-07-16 Utilisation de substances naturelles en tant qu'additifs alimentaires pour animaux aquatiques
JP2012520052A JP5881175B2 (ja) 2009-07-17 2010-07-16 水棲動物用飼料添加剤としての天然物質の使用
AU2010272494A AU2010272494B2 (en) 2009-07-17 2010-07-16 Use of natural substances as feed additives for aquatic animals

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Application Number Priority Date Filing Date Title
EP09165789.0 2009-07-17
EP09165789 2009-07-17

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WO2011006993A1 true WO2011006993A1 (fr) 2011-01-20

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EP (1) EP2453758A1 (fr)
JP (1) JP5881175B2 (fr)
CN (1) CN102469808B (fr)
AU (1) AU2010272494B2 (fr)
CA (1) CA2767873C (fr)
CL (1) CL2012000096A1 (fr)
WO (1) WO2011006993A1 (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
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CN103143553A (zh) * 2013-03-29 2013-06-12 广东大众农业科技股份有限公司 一种生物处理河道动物尸体的方法
EP2517568B1 (fr) * 2009-12-02 2015-10-28 Ewos Innovation AS Procédés et compositions d'alimentation pour masquer des produits sémiochimiques de poisson
EP3075261A1 (fr) * 2015-03-31 2016-10-05 Universidad De Santiago De Chile Formulation d'un complément alimentaire d'un extrait d'hypericum perforatum et rosmarinus officinalis (ro) ou d'un mélange des deux, qui améliore les barrières d'antioxydants endogènes, augmente le gain de poids dans l'élevage de poissons, et prolonge la durée de vie des poissons d'élevage affectés par des événements stressants et réduit une infection pathogène
WO2017064092A1 (fr) * 2015-10-13 2017-04-20 Dsm Ip Assets B.V. Additifs alimentaires pour animaux aquatiques comprenant des huiles essentielles et un lysozyme
CN108424814A (zh) * 2018-03-20 2018-08-21 甘肃烟草工业有限责任公司 一种枸杞香精的制备方法及在卷烟中的应用
EP3747280A1 (fr) * 2019-06-07 2020-12-09 Lucta, S.A. Combinaison antimicrobienne destinée à être utilisée dans l'alimentation des animaux
WO2022117810A1 (fr) * 2020-12-04 2022-06-09 Lucta, S.A. Additif alimentaire permettant d'augmenter le rapport des acides gras polyinsaturés oméga-3/oméga-6 chez les animaux aquatiques

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KR101746273B1 (ko) * 2015-02-25 2017-06-12 양정자 홍조색소(β-carotene)를 포함하는 전복 종묘용 사료조성물 제조방법
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CN105432972A (zh) * 2015-12-04 2016-03-30 全椒县城西畜牧养殖专业合作社 一种鲈鱼的富硒饲料添加剂
CN109258984A (zh) * 2018-10-08 2019-01-25 天津农学院 具有免疫促生长作用的肉桂醛在半滑舌鳎饲料中的应用
CN114009626A (zh) * 2021-10-21 2022-02-08 广东信豚生物科技有限公司 一种含酵母提取物的鱼粉替代物及水产动物预混料组合物

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Publication number Priority date Publication date Assignee Title
EP2517568B1 (fr) * 2009-12-02 2015-10-28 Ewos Innovation AS Procédés et compositions d'alimentation pour masquer des produits sémiochimiques de poisson
CN102217712A (zh) * 2011-05-27 2011-10-19 贺喜 含蒎烯的饲料或饲料添加剂
CN103143553A (zh) * 2013-03-29 2013-06-12 广东大众农业科技股份有限公司 一种生物处理河道动物尸体的方法
EP3075261A1 (fr) * 2015-03-31 2016-10-05 Universidad De Santiago De Chile Formulation d'un complément alimentaire d'un extrait d'hypericum perforatum et rosmarinus officinalis (ro) ou d'un mélange des deux, qui améliore les barrières d'antioxydants endogènes, augmente le gain de poids dans l'élevage de poissons, et prolonge la durée de vie des poissons d'élevage affectés par des événements stressants et réduit une infection pathogène
US10499664B2 (en) 2015-03-31 2019-12-10 Universidad De Santiago De Chile Fish feed formulation of Hypericum perforatum, Rosamarinus officianalis or a mixture thereof
WO2017064092A1 (fr) * 2015-10-13 2017-04-20 Dsm Ip Assets B.V. Additifs alimentaires pour animaux aquatiques comprenant des huiles essentielles et un lysozyme
CN108424814A (zh) * 2018-03-20 2018-08-21 甘肃烟草工业有限责任公司 一种枸杞香精的制备方法及在卷烟中的应用
EP3747280A1 (fr) * 2019-06-07 2020-12-09 Lucta, S.A. Combinaison antimicrobienne destinée à être utilisée dans l'alimentation des animaux
WO2020245333A1 (fr) * 2019-06-07 2020-12-10 Lucta, S.A. Combinaison antimicrobienne destinée à être utilisée dans des aliments pour animaux
WO2022117810A1 (fr) * 2020-12-04 2022-06-09 Lucta, S.A. Additif alimentaire permettant d'augmenter le rapport des acides gras polyinsaturés oméga-3/oméga-6 chez les animaux aquatiques

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CA2767873A1 (fr) 2011-01-20
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AU2010272494A1 (en) 2012-02-09
JP5881175B2 (ja) 2016-03-09
AU2010272494B2 (en) 2015-04-09
EP2453758A1 (fr) 2012-05-23
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CL2012000096A1 (es) 2012-07-06
CN102469808A (zh) 2012-05-23

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