WO2010133776A3 - Dispositif et procede d'isolement de cibles biologiques ou chimiques - Google Patents

Dispositif et procede d'isolement de cibles biologiques ou chimiques Download PDF

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Publication number
WO2010133776A3
WO2010133776A3 PCT/FR2010/000374 FR2010000374W WO2010133776A3 WO 2010133776 A3 WO2010133776 A3 WO 2010133776A3 FR 2010000374 W FR2010000374 W FR 2010000374W WO 2010133776 A3 WO2010133776 A3 WO 2010133776A3
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WO
WIPO (PCT)
Prior art keywords
chemical targets
biological
reaction chamber
relates
isolating biological
Prior art date
Application number
PCT/FR2010/000374
Other languages
English (en)
Other versions
WO2010133776A2 (fr
Inventor
Guillaume Durin
Sylvaine Chabaud
Cyril Delattre
Dorothée JARY
Original Assignee
Commissariat A L'energie Atomique Et Aux Energies Alternatives
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat A L'energie Atomique Et Aux Energies Alternatives filed Critical Commissariat A L'energie Atomique Et Aux Energies Alternatives
Priority to EP10727008A priority Critical patent/EP2433130A2/fr
Priority to US13/321,177 priority patent/US8703434B2/en
Priority to CN2010800272608A priority patent/CN102498402A/zh
Publication of WO2010133776A2 publication Critical patent/WO2010133776A2/fr
Publication of WO2010133776A3 publication Critical patent/WO2010133776A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention se rapporte à un dispositif de collecte et de concentration de cibles biologiques ou chimiques en vue de leur détection; le dispositif comprenant une chambre réactionnelle (1) dans laquelle sont placées des billes magnétiques fonctionnalisées (2), un système d'agitation par ultrasons (3), un champ magnétique (4) pouvant être activé (position ON) ou inactivé (position OFF) et un système de déplacement de fluide (5) pour l'introduction (51) et l'évacuation (52) de tout ou partie du contenu de la chambre réactionnelle. La présente invention se rapporte également à un procédé de capture de cibles biologiques ou chimiques mettant en œuvre ledit dispositif.
PCT/FR2010/000374 2009-05-19 2010-05-17 Dispositif et procede d'isolement de cibles biologiques ou chimiques WO2010133776A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP10727008A EP2433130A2 (fr) 2009-05-19 2010-05-17 Dispositif et procede d'isolement de cibles biologiques ou chimiques
US13/321,177 US8703434B2 (en) 2009-05-19 2010-05-17 Device and method for isolating biological or chemical targets
CN2010800272608A CN102498402A (zh) 2009-05-19 2010-05-17 用于分离生物或化学靶标的装置和方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0902383 2009-05-19
FR0902383A FR2945819B1 (fr) 2009-05-19 2009-05-19 Dispositif et procede d'isolement de cibles biologiques ou chimiques

Publications (2)

Publication Number Publication Date
WO2010133776A2 WO2010133776A2 (fr) 2010-11-25
WO2010133776A3 true WO2010133776A3 (fr) 2011-05-19

Family

ID=41435283

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2010/000374 WO2010133776A2 (fr) 2009-05-19 2010-05-17 Dispositif et procede d'isolement de cibles biologiques ou chimiques

Country Status (5)

Country Link
US (1) US8703434B2 (fr)
EP (1) EP2433130A2 (fr)
CN (1) CN102498402A (fr)
FR (1) FR2945819B1 (fr)
WO (1) WO2010133776A2 (fr)

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US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
CN102994383A (zh) * 2012-11-13 2013-03-27 陕西师范大学 磁-声复合作用的离体细胞标记装置和方法
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA2894694C (fr) 2012-12-14 2023-04-25 10X Genomics, Inc. Procedes et systemes pour le traitement de polynucleotides
US9644204B2 (en) 2013-02-08 2017-05-09 10X Genomics, Inc. Partitioning and processing of analytes and other species
US11029310B2 (en) 2013-03-14 2021-06-08 Wisconsin Alumni Research Foundation Device and method for extracting a targeted fraction from a sample
US10040062B2 (en) * 2014-01-14 2018-08-07 Wisconsin Alumni Research Foundation Device and method for transferring a target between locations
KR102596508B1 (ko) 2014-04-10 2023-10-30 10엑스 제노믹스, 인크. 시약을 캡슐화 및 구획화하기 위한 유체 디바이스, 시스템, 및 방법, 및 이의 응용
KR20230070325A (ko) 2014-06-26 2023-05-22 10엑스 제노믹스, 인크. 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법
CN107002128A (zh) 2014-10-29 2017-08-01 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10221436B2 (en) 2015-01-12 2019-03-05 10X Genomics, Inc. Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
US10697000B2 (en) * 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
EP3882357B1 (fr) 2015-12-04 2022-08-10 10X Genomics, Inc. Procédés et compositions pour l'analyse d'acide nucléique
WO2017197338A1 (fr) 2016-05-13 2017-11-16 10X Genomics, Inc. Systèmes microfluidiques et procédés d'utilisation
CN106111329B (zh) * 2016-08-16 2017-10-13 闫维新 一种微型磁珠的操控装置
ES2951666T3 (es) * 2016-09-12 2023-10-24 Hoffmann La Roche Procedimientos y composiciones para purificar ácidos nucleicos bicatenarios
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (fr) 2017-01-30 2023-06-28 10X Genomics, Inc. Procédés et systèmes de codage à barres de cellules individuelles sur la base de gouttelettes
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
EP3625361A1 (fr) 2017-11-15 2020-03-25 10X Genomics, Inc. Perles de gel fonctionnalisées
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CA3044782A1 (fr) 2017-12-29 2019-06-29 Clear Labs, Inc. Appareil d'amorcage et de chargement de repertoire automatises
CN112262218A (zh) 2018-04-06 2021-01-22 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CN109395875B (zh) * 2018-12-11 2023-10-20 苏州英赛斯智能科技有限公司 一种磁珠分离机构、装置以及磁珠分离方法
CN110208520A (zh) * 2019-06-27 2019-09-06 深圳华迈兴微医疗科技有限公司 一种主动液流控制微流控检测系统
CN110208519A (zh) * 2019-06-27 2019-09-06 深圳华迈兴微医疗科技有限公司 一种主动液流控制微流控检测系统
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Also Published As

Publication number Publication date
FR2945819B1 (fr) 2011-06-17
US20120190037A1 (en) 2012-07-26
FR2945819A1 (fr) 2010-11-26
EP2433130A2 (fr) 2012-03-28
CN102498402A (zh) 2012-06-13
WO2010133776A2 (fr) 2010-11-25
US8703434B2 (en) 2014-04-22

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