MY150966A - Lateral flow device and method of detection of nucleic acid sequence - Google Patents

Lateral flow device and method of detection of nucleic acid sequence

Info

Publication number
MY150966A
MY150966A MYPI2010700012A MY150966A MY 150966 A MY150966 A MY 150966A MY PI2010700012 A MYPI2010700012 A MY PI2010700012A MY 150966 A MY150966 A MY 150966A
Authority
MY
Malaysia
Prior art keywords
region
nucleic acid
acid sequence
detection
lateral flow
Prior art date
Application number
Inventor
Chan Yean Yean
Chua Ang Lim
Lalitha Pattabiraman
Manickam Ravichandran
Lim Boon Huat
Original Assignee
Universiti Sains Malaysia Usm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiti Sains Malaysia Usm filed Critical Universiti Sains Malaysia Usm
Priority to MYPI2010700012 priority Critical patent/MY150966A/en
Priority to PCT/MY2010/000212 priority patent/WO2011112068A1/en
Publication of MY150966A publication Critical patent/MY150966A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

THE PRESENT INVENTION RELATES TO A LATERAL FLOW DEVICE (1) FOR DETECTING THE PRESENCE OR ABSENCE OF AT LEAST ONE TARGET NUCLEIC ACID SEQUENCE (13) BEARING A FIRST HAPTEN LABEL (14) AND A SECOND HAPTEN LABEL (15), COMPRISING A) A CONJUGATES REGION (3) CONTAINING A DETECTOR CONJUGATE (9) WHICH BINDS TO THE SECOND HAPTEN LABEL (15) OF THE TARGET NUCLEIC ACID SEQUENCE (13) AND IS PROVIDED WITH A SIGNALLING LABEL; B) AN APPLICATION REGION (2) ADJACENT TO SAID CONJUGATES REGION (3); C) A DETECTION REGION (4) ADJACENT TO SAID APPLICATION REGION (2), SAID DETECTION REGION (4) CONTAINING AT LEAST ONE CAPTURE SUB-REGION (6) CONTAINING A CAPTURE REAGENT (10) IMMOBILIZED IN THE CAPTURE SUB-REGION (6), WHICH BINDS TO THE FIRST HAPTEN LABEL (14) OF THE TARGET NUCLEIC ACID SEQUENCE (13); D) AN ADSORBENT REGION (5) ADJACENT TO SAID DETECTION REGION. FURTHER, IT PROVIDES A CHROMATOGRAPHIC CONTROL OF THE WORKABILITY OF THE DEVICE AND FOR AN INTERNAL AMPLIFICATION CONTROL THAT VERIFIES A NUCLEIC ACID AMPLIFICATION ASSAY. IN ADDITION TO THAT, THE PRESENT INVENTION RELATES TO A METHOD USING SAID DEVICE.
MYPI2010700012 2010-03-08 2010-03-08 Lateral flow device and method of detection of nucleic acid sequence MY150966A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
MYPI2010700012 MY150966A (en) 2010-03-08 2010-03-08 Lateral flow device and method of detection of nucleic acid sequence
PCT/MY2010/000212 WO2011112068A1 (en) 2010-03-08 2010-10-21 Lateral flow device and method of detection of nucleic acid sequence

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MYPI2010700012 MY150966A (en) 2010-03-08 2010-03-08 Lateral flow device and method of detection of nucleic acid sequence

Publications (1)

Publication Number Publication Date
MY150966A true MY150966A (en) 2014-03-31

Family

ID=44563701

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2010700012 MY150966A (en) 2010-03-08 2010-03-08 Lateral flow device and method of detection of nucleic acid sequence

Country Status (2)

Country Link
MY (1) MY150966A (en)
WO (1) WO2011112068A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103134933B (en) * 2013-02-05 2015-07-01 江西中德生物工程有限公司 Test strip used for detecting vibrio parahemolytocus and purpose thereof
EP2966177A1 (en) 2014-07-09 2016-01-13 Vetgenomics, S.L. Methods for detecting target DNA sequences
CN104849453A (en) * 2015-05-22 2015-08-19 天津科技大学 Preparation method of colloidal carbon labeled antibody and test strip prepared from colloidal carbon labeled antibody
WO2017034019A1 (en) * 2015-08-26 2017-03-02 株式会社カネカ Nucleic acid detection device and nucleic acid detection method
CN107300617A (en) * 2017-07-17 2017-10-27 广东志道医药科技有限公司 The inhibitor colloidal gold immuno-chromatography test paper strips of PDE 5 and its preparation and application
CN109142718B (en) * 2018-05-08 2021-09-10 武汉纳达康生物科技有限公司 Method for preparing amino colloidal carbon and labeled antibody thereof by one-step method
US10914731B2 (en) * 2019-02-21 2021-02-09 Qinxue DING Method of removing non-specific binding signals using microparticle assay
CN110794130A (en) * 2019-10-22 2020-02-14 中科佑隆（杭州）食安标准科技有限公司 Nucleic acid two-in-one immune gold-labeled rapid detection card, preparation method and detection method thereof
CN112899139A (en) * 2021-01-14 2021-06-04 北京普若博升生物科技有限公司 Nucleic acid test strip card box and use method and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050227275A1 (en) * 2004-04-07 2005-10-13 Access Bio, Inc. Nucleic acid detection system
US20100136531A1 (en) * 2006-04-10 2010-06-03 Tecra International Pty Ltd Nucleic acid detection using lateral flow methods
US20090142757A1 (en) * 2007-11-30 2009-06-04 Asiagen Corporation Strip and method for detecting nucleotide amplification products of mycobacterium tuberculosis and non-tuberculous mycobacterium

Also Published As

Publication number Publication date
WO2011112068A1 (en) 2011-09-15

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