WO2010122405A2 - Procédé permettant la surexpression de constituants chimiques chez des végétaux - Google Patents

Procédé permettant la surexpression de constituants chimiques chez des végétaux Download PDF

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Publication number
WO2010122405A2
WO2010122405A2 PCT/IB2010/000894 IB2010000894W WO2010122405A2 WO 2010122405 A2 WO2010122405 A2 WO 2010122405A2 IB 2010000894 W IB2010000894 W IB 2010000894W WO 2010122405 A2 WO2010122405 A2 WO 2010122405A2
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WO
WIPO (PCT)
Prior art keywords
plants
gene
plant
explants
dioscorea
Prior art date
Application number
PCT/IB2010/000894
Other languages
English (en)
Other versions
WO2010122405A3 (fr
WO2010122405A4 (fr
Inventor
Smitha Sushama Thankappan
Villoo Morawala Patell
Original Assignee
Avesthagen Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avesthagen Limited filed Critical Avesthagen Limited
Publication of WO2010122405A2 publication Critical patent/WO2010122405A2/fr
Publication of WO2010122405A3 publication Critical patent/WO2010122405A3/fr
Publication of WO2010122405A4 publication Critical patent/WO2010122405A4/fr

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention apporte une solution à la problématique de la surexpression de constituants chimiques chez des végétaux. L'invention concerne un procédé permettant d'obtenir des végétaux modifiés ayant une efficacité de régénération accrue, et rendant possible la surexpression de constituants chimiques chez des végétaux, de préférence la sapogénine stéroïdique, la diosgénine chez Dioscorea prazeri. Le procédé comprend une modification génétique médiée par Agrobacterium tumeficians.
PCT/IB2010/000894 2009-04-23 2010-04-22 Procédé permettant la surexpression de constituants chimiques chez des végétaux WO2010122405A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2586/CHE/2008 2009-04-23
IN2586CH2008 2009-04-23

Publications (3)

Publication Number Publication Date
WO2010122405A2 true WO2010122405A2 (fr) 2010-10-28
WO2010122405A3 WO2010122405A3 (fr) 2010-12-16
WO2010122405A4 WO2010122405A4 (fr) 2011-03-10

Family

ID=43011532

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2010/000894 WO2010122405A2 (fr) 2009-04-23 2010-04-22 Procédé permettant la surexpression de constituants chimiques chez des végétaux

Country Status (1)

Country Link
WO (1) WO2010122405A2 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589619A (en) * 1990-10-12 1996-12-31 Amoco Corporation Process and composition for increasing squalene and sterol accumulation in higher plants
US20050102716A1 (en) * 1999-04-12 2005-05-12 Mylavarapu Venkatramesh Transgenic plants containing altered levels of sterol compounds and tocopherols

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589619A (en) * 1990-10-12 1996-12-31 Amoco Corporation Process and composition for increasing squalene and sterol accumulation in higher plants
US20050102716A1 (en) * 1999-04-12 2005-05-12 Mylavarapu Venkatramesh Transgenic plants containing altered levels of sterol compounds and tocopherols

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BAE T. W. ET AL: 'Agrobacterium tumefaciens-mediated transformation of a medicinal plant Taraxacum platycarpum' PLANT CELL, TISSUE AND ORGAN CULTURE vol. 80, 2005, pages 51 - 57 *
DATABASE HCAPLUS 'Enhancement of Hevea crop potential by genetic transformation: HMGR activity in transformed tissue' Database accession no. 1997:150017 & SYMPOSIUM ON PHYSIOLOGICAL AND MOLECULAR ASPECTS OF THE BREEDING OF HEVEA BRASILIENSIS 06 November 1995 - 07 November 1995, PENANG, MALAYSIA, *

Also Published As

Publication number Publication date
WO2010122405A3 (fr) 2010-12-16
WO2010122405A4 (fr) 2011-03-10

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