WO2010100724A1 - Procédé et appareil de prétraitement pour l'électrophorèse - Google Patents

Procédé et appareil de prétraitement pour l'électrophorèse Download PDF

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Publication number
WO2010100724A1
WO2010100724A1 PCT/JP2009/054024 JP2009054024W WO2010100724A1 WO 2010100724 A1 WO2010100724 A1 WO 2010100724A1 JP 2009054024 W JP2009054024 W JP 2009054024W WO 2010100724 A1 WO2010100724 A1 WO 2010100724A1
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WIPO (PCT)
Prior art keywords
sample
reservoir
buffer
obstacle
buffer solution
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PCT/JP2009/054024
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English (en)
Japanese (ja)
Inventor
徹 加地
Original Assignee
株式会社島津製作所
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Application filed by 株式会社島津製作所 filed Critical 株式会社島津製作所
Priority to PCT/JP2009/054024 priority Critical patent/WO2010100724A1/fr
Publication of WO2010100724A1 publication Critical patent/WO2010100724A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

Definitions

  • the present invention relates to a pretreatment method and apparatus in electrophoretic analysis for analyzing a very small amount of protein, nucleic acid, drug and the like using an electrophoresis plate in fields such as biochemistry, molecular biology, and clinical practice.
  • Electrophoresis plates have been proposed and used in order to facilitate the handling of the apparatus and to realize high-speed analysis and miniaturization of the apparatus (see Patent Documents 1 and 2).
  • the electrophoresis plate is provided with one or a plurality of flow paths composed of capillaries for separating a sample in a substrate.
  • the electrophoresis plate targeted by the present invention includes both one and a plurality of capillaries formed inside the substrate.
  • the capillary formed inside is used as a separation flow path for electrophoresis.
  • electrophoretic analysis using an electrophoresis plate there is a sample introduction step into the separation channel as a pretreatment for analysis.
  • a method for introducing a sample into the separation channel there are a cross injection method having a sample introduction channel intersecting with the separation channel and a method for introducing a sample from one end of the separation channel.
  • the sample introduction method targeted by the present invention is a method of introducing a sample from one end of the latter separation channel.
  • one opening at both ends of the capillary opened on the substrate surface serves as a sample injection reservoir.
  • the sample injection reservoir is formed at the bottom of the buffer reservoir that stores a buffer solution that comes into contact with the electrode during electrophoretic separation.
  • a sample solution is injected into a sample injection reservoir in a state where the capillary is filled with a separation medium, and the sample is sandwiched between the samples.
  • a voltage is applied between the reservoir filled with the buffer solution on the side opposite to the side and the sample solution, and the sample is introduced into the capillary by electrophoresis.
  • the sample injection reservoir is washed. After washing, the reservoir is filled with a buffer solution, and then a voltage is applied from both ends of the capillary to perform electrophoretic analysis of the sample.
  • the sample injection reservoir is washed after sample injection.
  • a method for removing the sample remaining in the sample injection reservoir after the sample is introduced from the sample injection reservoir into the electrophoresis medium in the capillary a method called a suction removal method instead of cleaning is being studied.
  • a suction removal method after a sample is introduced into the electrophoresis medium, a buffer solution is injected into a buffer reservoir provided with a sample injection reservoir at the bottom.
  • the sample solution remaining in the sample injection reservoir is removed by aspirating and removing a solution larger than the volume of the sample injection reservoir, for example, twice the volume of the sample injection reservoir from the sample injection reservoir. To do. Thereafter, a voltage is applied from both ends of the capillary to perform an electrophoretic analysis of the sample.
  • This suction removal method for removing the sample remaining in the sample injection reservoir is a novel method and has less influence on the end surface of the separation medium facing the reservoir than the cleaning method.
  • the electrode is brought into contact with the buffer solution in the buffer reservoir 12 and the buffer solution in the buffer reservoir on the opposite side across the flow path 6, and a voltage is applied between both electrodes to cause the sample of the sample plug 18 to be separated by the separation medium 14. Electrophoretically separate.
  • step (c) When supplying the buffer solution 24 to the buffer reservoir 12 in step (c), if the buffer solution 24 flows into the sample reservoir 8 vigorously, the end surface of the sample plug 18 facing the sample reservoir 8 is disturbed. When such a situation occurs, the electrophoretic separation performance is deteriorated.
  • the present invention relates to an improvement in a method for replacing a residual sample solution in a sample reservoir, and prevents the end face of the sample plug from being disturbed by the flow of the buffer solution when the residual sample solution in the sample reservoir is replaced with a buffer solution.
  • the object is to improve the separation performance of electrophoresis.
  • the present invention is a pretreatment method for electrophoretic analysis using an electrophoretic plate having a flow path composed of capillaries for separating a sample.
  • the electrophoresis plate to be used includes a first buffer reservoir for containing a buffer solution on one end side of the flow path, and a sample reservoir for introducing a sample formed at the bottom of the buffer reservoir and connected to the flow path.
  • a second buffer reservoir for containing a buffer solution is provided at the other end.
  • This pretreatment method includes the following steps (A) to (D).
  • A filling the flow path with a separation medium;
  • B filling the sample reservoir with a sample solution and filling the second buffer reservoir with a buffer solution;
  • C An electrode is brought into contact with the sample solution and the buffer solution filled in the step (B), a voltage is applied between both electrodes to introduce the sample into the separation medium, and an end of the separation medium on the sample reservoir side And
  • D removing the electrode on the sample side from the sample reservoir and then supplying the buffer solution to the first buffer reservoir to remove the sample solution remaining in the sample reservoir. Step of replacing with buffer solution.
  • the filling of the buffer solution into the first buffer reservoir in the step (D) obstructs the opening of the sample reservoir so that the gap through which the buffer solution flows into the sample reservoir is 0.5 to 3.0 mm. This is done with the objects placed.
  • An example of an obstacle is a lid member that covers the opening of the sample reservoir. In that case, it is achieved by arranging the lid member at such a height that the distance between the front end surface of the lid member and the edge of the opening of the sample reservoir is 0.5 to 3.0 mm in the gap through which the buffer solution flows. Is done. Such an obstacle is arranged so that the tip surface thereof is located above the opening of the sample reservoir.
  • Another example of the obstacle is a rod-like body having a similar cross-sectional shape smaller than the opening of the sample reservoir, and the size of the cross-sectional shape of the rod-like body when the rod-like body is inserted into the opening.
  • the distance between the side surface of the rod-like body and the edge of the opening of the sample reservoir is such that the gap is 0.5 to 3.0 mm.
  • it is arranged so that the tip of the rod-like body is inserted into the opening of the sample reservoir.
  • Step (D) is preferably performed in a state where the inner surface of the sample reservoir is hydrophilically treated. In that case, it is preferable that the bottom surface of the first buffer reservoir is also hydrophilically treated.
  • the hydrophilic treatment method for example, a method using a bifunctional compound having a first functional group that reacts with the glass surface and a second functional group that polymerizes can be employed (see Patent Document 3).
  • the second functional group is polymerized by bonding a monomer for imparting hydrophilicity to the second functional group.
  • a monomolecular layer of a hydrophilic polymer is formed on the glass surface.
  • this method for example, when ⁇ -methacryloxypropyltrimethoxysilane is used as a bifunctional compound and acrylamide is bonded as a monomer, the glass surface can be covered with a monolayer of polyacrylamide. .
  • the pretreatment apparatus of the present invention is for realizing the process after the flow path is filled with the separation medium in the pretreatment method of the present invention, and the electrophoresis plate filled with the separation medium is mounted thereon.
  • a substrate holding unit, a sample supply unit for supplying a sample solution to the sample reservoir of the electrophoresis plate placed on the substrate holding unit, and a first buffer reservoir of the electrophoresis plate placed on the substrate holding unit A first buffer solution supply unit configured to supply a buffer solution; a second buffer solution supply unit configured to supply a buffer solution to a second buffer reservoir of the electrophoresis plate placed on the substrate holding unit; a sample solution; and a second buffer reservoir.
  • a voltage application unit including an electrode and a power supply device for applying a voltage when applying a voltage to the buffer solution and introducing a sample into the separation medium and forming a sample plug at the end of the separation medium on the sample reservoir side
  • the gap through which the buffer solution flows into the sample reservoir is 0.5 to 3.0 mm, or within the opening of the sample reservoir or above the opening.
  • an obstacle placement section for placing obstacles.
  • the obstacle placement unit When the obstacle is a lid member that covers the opening of the sample reservoir, the obstacle placement unit includes a moving mechanism that moves the lid member in the vertical direction, and the first buffer solution supply unit buffers the first buffer reservoir.
  • the lid member When the liquid is supplied, the lid member is lowered, and the lid member is arranged at a height where the distance between the front end surface of the lid member and the edge of the opening becomes the gap into which the buffer liquid flows.
  • the obstacle placement unit may place the obstacle at a position where the tip surface is above the opening of the sample reservoir.
  • the obstacle is a rod-like body having a similar cross-sectional shape that is smaller than the opening of the sample reservoir, and the size of the cross-sectional shape is such that when the obstacle is inserted into the sample reservoir,
  • the obstacle placement unit includes a moving mechanism for moving the obstacle in the vertical direction, and the first buffer liquid supply unit is the first buffer reservoir.
  • the injection port for discharging the buffer solution of the first buffer solution supply unit may be configured separately from the obstacle arrangement unit, or the obstacle arrangement unit so as to move in the vertical direction simultaneously with the obstacle. It may be attached to.
  • the buffer solution can be supplied to the sample reservoir without disturbing the end surface of the sample plug on the sample reservoir side, thereby improving the separation performance without causing deterioration of the plug shape.
  • FIG. 1B is an enlarged cross-sectional view showing a sample reservoir and a buffer reservoir on the cathode side in the electrophoresis plate of FIG. 1A.
  • FIG. 2 is a cross-sectional view showing an example of an obstacle applied to an electrophoresis plate having a plurality of flow paths.
  • Electrophoresis plate 6 Capillary 8 Sample reservoir 10 Anode-side buffer reservoir (second buffer reservoir) 12 Cathode side buffer reservoir (first buffer reservoir) DESCRIPTION OF SYMBOLS 13 Electrode 16 Sample solution 18 Sample plug by the introduced sample 20, 20a, 20b Obstacle 22 Buffer liquid injection
  • the electrophoresis plate 2 has only one separation channel for separating a sample.
  • the capillary 6 as a separation channel is formed inside the glass substrate 3.
  • the capillary 6 has a width of 50 to 150 ⁇ m, a height of 20 to 100 ⁇ m, and a length of 10 to 500 mm, and is filled with hydroxyethyl cellulose, polyvinyl pyrrolidone, polydimethylacrylamide, polyacrylamide or the like as a separation medium.
  • a buffer reservoir (first buffer reservoir) 12 that stores a buffer solution is formed on the substrate surface on one end side (cathode side) of the capillary 6.
  • the buffer reservoir 12 has a cylindrical member fixed to the surface of the substrate 3.
  • a sample reservoir 8 is formed at the bottom of the buffer reservoir 12 to store the sample solution for injecting the sample into the separation medium filled in the capillary 6.
  • the sample reservoir 8 is connected to one end of the capillary 6 and is formed as a recess opened on the surface of the substrate 3.
  • the sample reservoir 8 has a cylindrical shape with a depth of 0.5 to 2 mm and
  • the inner surfaces of the buffer reservoir 12 and the sample reservoir 8 are subjected to surface treatment so as to be hydrophilic.
  • surface treatment for hydrophilicity ⁇ -methacryloxypropyltrimethoxysilane was bonded to the glass surface, and then the acrylamide solution was allowed to act to cover the glass surface with a polyacrylamide monolayer.
  • a buffer reservoir (second buffer reservoir) 10 that stores a buffer solution is formed on the substrate surface on the other end side (anode side) of the capillary 6.
  • the buffer reservoir 10 also has a cylindrical member fixed to the surface of the substrate 3.
  • a concave portion 9 is also formed at the bottom of the buffer reservoir 10, which is connected to the other end of the capillary 6 and opened on the surface of the substrate 3.
  • the size of the recess 9 may be the same as or different from the size of the sample reservoir 8.
  • the substrate 3 is a laminate of two glass substrates, the capillary 6 is formed as a groove on one of the opposing surfaces of the two substrates, and the sample reservoir 8 and the recess 9 are formed as through holes in one substrate. It is formed as.
  • FIG. 2 is a cross-sectional view showing the vicinity of the sample reservoir of the electrophoresis plate in order of steps in order to explain the pretreatment procedure in the electrophoresis analysis using the electrophoresis plate shown in FIGS. 1A to 1C.
  • the obstacle 20 is placed above the sample reservoir 8.
  • the obstacle 20 has a bottom area larger than the opening of the sample reservoir 8. Its bottom area is a flat surface.
  • the position where the obstacle 20 is disposed is a position where the gap through which the buffer solution flows into the sample reservoir 8 is 0.5 to 3.0 mm.
  • the buffer solution 24 is supplied from the supply port 22 of the buffer solution supply unit to the buffer reservoir 12 with the obstacle 20 placed above the sample reservoir 8.
  • the buffer solution 24 enters the sample reservoir 8 through the gap between the obstacle 20 and the sample reservoir 8.
  • the electrodes After removing the suction nozzle, the electrodes are brought into contact with the buffer solution 24 in the buffer reservoir 12 on the opposite side across the flow path 6 with the buffer solution 24 in the buffer reservoir 12, and a voltage is applied between the electrodes to apply the sample plug 18.
  • the sample is electrophoresed and separated by the separation medium 14.
  • the sample components separated by electrophoresis are detected at an appropriate position on the anode side of the flow path 6 (the side opposite to the side where the sample reservoir 8 is present).
  • the detection is performed, for example, by a fluorescence measurement unit that detects fluorescence generated by irradiating the flow path 6 with excitation light.
  • TTE 1-fold concentration TTE (TRIS-TAPS-EDTA) solution
  • TRIS and TAPS are buffer agents
  • EDTA is ethylenediaminetetraacetic acid.
  • the sample solution 16 remaining in the sample reservoir 8 can be replaced with the buffer solution 24 without disturbing the end face of the sample plug 18 formed in the separation medium in the capillary 6.
  • 3A-3C show some examples of obstacles.
  • FIG. 3A represents the obstacle 20 shown in FIG.
  • the obstacle 20 is a lid member that is disposed above the opening of the sample reservoir 8 and covers the opening.
  • the front end surface 21 of the obstacle 20 is a flat surface, and the front end surface 21 is similar to the opening of the sample reservoir 8.
  • a distance L between the front end surface 21 and the edge of the opening is a gap into which the buffer solution flows.
  • the size of the distal end surface 21 may be larger or smaller than the opening of the sample reservoir 8, but if it is small, it cannot be so small that a predetermined gap cannot be formed.
  • the obstacle 20a in FIG. 3B is a curved surface that is curved so that its tip surface 21a is convex in the tip direction. Also in this case, the distance L between the tip surface 21a and the edge of the opening is a gap into which the buffer solution flows. If the tip surface 21a is curved, the flow of the buffer solution flowing into the sample reservoir 8 is stabilized.
  • 3C is a rod-like body having a similar cross-sectional shape smaller than the opening of the sample reservoir 8.
  • the size of the cross-sectional shape of the obstacle 20c is such that when the obstacle 20b is inserted into the opening of the sample reservoir 8, the distance L between the side surface of the obstacle 20b and the edge of the opening becomes a gap into which the buffer solution flows. Is set to In this case, the distance L is preferably in the range of 0.5 mm to 3.0 mm, and preferably not more than 1 ⁇ 2 of the diameter of the opening of the sample reservoir 8.
  • These obstacles 20, 20a, 20b are automatically arranged above or in the opening of the sample reservoir 12 when the buffer solution is supplied to the sample reservoir 12.
  • FIG. 4 shows a first example of the obstacle placement unit for placing the obstacles 20, 20a, 20b.
  • FIG. 4 shows a state after the buffer liquid 24 is supplied to the buffer reservoir 12.
  • the electrophoresis plate 2 in which the flow path is filled with the separation medium 14 is disposed on the substrate holding unit 25 of the pretreatment apparatus of the present invention, and the separation medium 14 is transferred from the sample reservoir 12.
  • the sample is introduced to form a sample plug 18 and the sample solution remains in the sample reservoir 12.
  • the obstacle placement unit includes a moving mechanism 26 that is driven by a motor to move the support rod 28 in the vertical direction.
  • the obstacle 20 is fixed to the tip of the support bar 28 and can be moved in the vertical direction.
  • the injection nozzle 22 which is an injection port for discharging the buffer solution 24 is fixed to the support rod 28 together with the obstacle 20 so as to move in the vertical direction simultaneously with the obstacle 20.
  • the obstacle 20 When supplying the buffer solution 24 to the buffer reservoir 12, as shown in FIG. 4A, the obstacle 20 is lowered and positioned at a predetermined position with respect to the opening of the sample reservoir 12. At this time, the injection nozzle 22 is also lowered and positioned at a predetermined position for supplying the buffer liquid 24 to the buffer reservoir 12. When the buffer liquid 24 is discharged from the injection nozzle 22 in this state, the buffer liquid 24 enters the sample reservoir 12 through the gap between the opening of the sample reservoir 12 and the obstacle 20.
  • FIG. 5 shows the same mechanism as FIG. 4 for positioning the obstacle 20 by moving it vertically.
  • the injection nozzle 22 is fixed at a predetermined position with respect to the buffer reservoir 12. Only the obstacle 20 moves up and down.
  • FIG. 5 shows the case where the obstacle shown in FIG. 3A is used as an obstacle, but it can also be applied to the case where the one shown in FIG. 3B or 3C is used.
  • FIGS. 6A to 6C show an example of an electrophoresis plate having a plurality of flow paths.
  • a plurality of capillaries 6 are formed as separation channels for separating a sample.
  • a sample reservoir 8 formed on one end side (cathode side) of each capillary 6 is formed on the bottom of a common buffer reservoir 12 formed on the surface of the substrate 3 and having a larger capacity than the sample reservoir 8.
  • a recess 9 on the other end side (anode side) of each capillary 6 is shown in FIGS. 1A to 1C for each capillary 6 arranged at the bottom of a common reservoir 10 formed on the surface of the substrate 3. Same as the electrophoresis plate.
  • the obstacle 20 c has as many tip surfaces as the plurality of sample reservoirs 8 arranged in the buffer reservoir 12, and supplies the buffer 24 to the buffer reservoir 12.
  • the sample reservoirs 8 are positioned at predetermined positions in the openings.
  • Only one injection nozzle 22 for supplying the buffer 24 is provided for the buffer reservoir 12.
  • the injection nozzle 22 may be attached to the obstacle arrangement portion so as to move in the vertical direction together with the obstacle 20c, or may be fixed to a predetermined position of the buffer reservoir 12.
  • FIG. 8 shows an example of an electrophoretic analyzer that includes the pretreatment device of one embodiment and performs the electrophoretic analysis after the separation medium is filled in the electrophoretic plate in the pretreatment.
  • the electrophoretic analyzer 122 includes a substrate holding unit (described in FIGS. 4 and 5), a sample supply unit 124, a second buffer solution supply unit 126, a first buffer solution supply unit 128, and a voltage application unit 130. , A fluorescence measurement unit 132, an obstacle arrangement unit 133, and a control unit 134 are provided.
  • the electrophoresis plate 136 in a state where the flow path is filled with the separation medium is placed on the substrate holder.
  • the sample supply unit 124 supplies a sample solution prepared in advance by an analyst at a predetermined position to the sample reservoir of the electrophoresis plate 136 placed on the substrate holding unit.
  • the second buffer solution supply unit 126 supplies the buffer solution to the anode buffer reservoir (second buffer reservoir) of the electrophoresis plate 136 placed on the substrate holding unit.
  • the first buffer solution supply unit 128 supplies the buffer solution to the cathode buffer reservoir (first buffer reservoir) of the electrophoresis plate 136 placed on the substrate holding unit.
  • the voltage application unit 130 applies a voltage to the sample solution and the buffer solution of the second buffer reservoir to introduce the sample into the separation medium to form a sample plug at the end of the separation medium on the sample reservoir side, It includes an electrode and a power supply device for applying a voltage when the voltage is applied between the buffer solutions in the second buffer reservoir and the sample of the sample plug is electrophoretically separated by the separation medium.
  • the fluorescence measuring unit 132 is for detecting the sample component electrophoretically separated by the capillary.
  • the obstacle placement unit 133 is configured so that the gap in which the buffer solution flows into the sample reservoir when the first buffer solution supply unit 128 supplies the buffer solution to the first buffer reservoir is 0.5 to 3.0 mm. Place obstacles in the openings.
  • the control unit 134 controls operations of the sample supply unit 124, the buffer solution supply unit 126, the replacement solution supply unit 128, the voltage application unit 130, the fluorescence measurement unit 132, and the obstacle arrangement unit 133.
  • control unit 134 is connected to a personal computer (PC) 138 so that the analyst can operate while looking at the monitor.
  • PC personal computer
  • the sample supply unit 124, the second buffer solution supply unit 126, and the first buffer solution supply unit 128 are provided with a tank for storing each solution and a solution pump for supplying them.
  • the control unit 34 controls, for example, the following operation.
  • the capillary of the electrophoresis plate 136 placed on the substrate holder is already filled with the separation medium.
  • the sample solution is supplied to the sample reservoir of the electrophoresis plate 136 by the sample supply unit 124, and a voltage is applied from the electrode by the voltage application unit 130 to introduce a part of the sample into the separation medium in the capillary.
  • the obstacle placement unit 133 places the obstacle in a predetermined position of the opening of the sample reservoir, and the first buffer solution supply unit 128 supplies the buffer solution from the buffer reservoir on the sample side to the sample reservoir. The obstacle is removed from the sample reservoir by the obstacle placement unit 133.
  • a voltage is applied between both ends of the capillary by the voltage application unit 126, and the sample is electrophoretically separated in the capillary.
  • the sample component electrophoretically separated by the capillary is detected by the fluorescence measuring unit 132, and the data is transmitted to the PC 138.
  • the analyst places the electrophoresis plate 136 filled with the separation medium on the substrate holding unit, prepares the sample solution and the buffer solution at predetermined positions, and causes the control unit 134 to execute the control method as described above.
  • the electrophoresis analyzer can automatically perform from pretreatment to analysis.
  • FIG. 9 shows the result of electrophoretic separation using the electrophoresis plate 2 of FIGS. 1A to 1C.
  • the black triangle data are for the example in which the obstacle 20 shown in FIG. 3A is arranged.
  • the obstacle 20 was larger than the opening of the sample reservoir 8 and was arranged so that the distance L between the edge of the opening and the front end surface of the obstacle 20 was about 0.5 mm.
  • the white square data is a comparative example in which such an obstacle is not arranged.
  • the horizontal axis in this graph represents the number of bases corresponding to the elapsed time from the start of measurement, and the vertical axis represents the degree of separation.
  • an electrophoresis plate 2 having a volume of the sample reservoir 8 of 2.5 ⁇ L (microliter) was used.
  • a sample solution a solution obtained by dissolving an ethanol-purified reaction product using BigDye (trademark) Terminator V3.1 Cycle Sequencing Kit (product of Applied Biosystems) in a solution containing 50% ethylene glycol was used.
  • the buffer solution supplied from the second buffer solution supply unit 126 of FIG. 8 50 mM Tris / 50 mM TAPS / 2 mM EDTA was used.
  • a solution having the same composition was used as the buffer solution supplied from the first buffer solution supply unit 128.
  • the degree of separation can be improved by placing an obstacle at the opening of the sample reservoir. .
  • the improvement in the separation degree in the long chain region is remarkable.

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Abstract

Selon l'invention, une solution échantillon est délivrée à un réservoir d'échantillon pour introduire l'échantillon dans un milieu de séparation disposé dans un trajet d'écoulement, après quoi une solution tampon est délivrée au réservoir d'échantillon. La solution tampon est délivrée au réservoir d'échantillon dans un état dans lequel un obstacle est disposé dans une partie d'ouverture du réservoir d'échantillon, de telle sorte qu'un espace à travers lequel la solution tampon s'écoule dans le réservoir d'échantillon a une taille de 0,5 à 3,0 mm.
PCT/JP2009/054024 2009-03-04 2009-03-04 Procédé et appareil de prétraitement pour l'électrophorèse WO2010100724A1 (fr)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002310990A (ja) * 2001-04-13 2002-10-23 Shimadzu Corp 電気泳動装置
JP2003166975A (ja) * 2001-11-30 2003-06-13 Shimadzu Corp 電気泳動部材用リザーバ部材及び電気泳動部材

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002310990A (ja) * 2001-04-13 2002-10-23 Shimadzu Corp 電気泳動装置
JP2003166975A (ja) * 2001-11-30 2003-06-13 Shimadzu Corp 電気泳動部材用リザーバ部材及び電気泳動部材

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