WO2010059206A2 - Compositions de nucléotides modulaires et utilisations de celles-ci - Google Patents
Compositions de nucléotides modulaires et utilisations de celles-ci Download PDFInfo
- Publication number
- WO2010059206A2 WO2010059206A2 PCT/US2009/006177 US2009006177W WO2010059206A2 WO 2010059206 A2 WO2010059206 A2 WO 2010059206A2 US 2009006177 W US2009006177 W US 2009006177W WO 2010059206 A2 WO2010059206 A2 WO 2010059206A2
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- WO
- WIPO (PCT)
- Prior art keywords
- composition
- group
- linkage
- covalent
- coupled
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- 0 CCC(CC)(CCOCCOCCOC(C)(C)CNC(CCCCCCC(NCCCCCCOP(O)(OP(O)(OP(O)(OP(O)(OP(O)(OP(O)(OC[C@](C(C1)O)O[C@]1N(C=C(C)C(N1)=O)C1=O)=O)=O)=O)=O)=O)=O)=O)=O)OCCOCCNC(*)=O Chemical compound CCC(CC)(CCOCCOCCOC(C)(C)CNC(CCCCCCC(NCCCCCCOP(O)(OP(O)(OP(O)(OP(O)(OP(O)(OP(O)(OC[C@](C(C1)O)O[C@]1N(C=C(C)C(N1)=O)C1=O)=O)=O)=O)=O)=O)=O)=O)=O)OCCOCCNC(*)=O 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
Definitions
- the functional group is coupled to the polyphosphate groups through a configurable linkage that allows in situ attachment of the functional group to the polyphosphate chain.
- the linkage included within the compositions of the invention allows for a cassette approach to the selection and configuration of nucleotides in order to tailor such compositions for a desired application.
- This cassette approach takes advantage of a facile linkage process which does not require the use of complex synthetic schemes to covalently couple functional groups to nucleotide compositions.
- Use of such facile linkage processes permits one to rapidly choose and link a desired functional group to a nucleotide for use in a desired application. Further, one can configure sets of nucleotides according to a wide variety of application specific criteria.
- a schematic illustration of the cassette-based linkage process is illustrated in Figure 1.
- Figure 6A, B and C show detection of incorporation of: A568-SAv-Biotin-dT6P in conjunction with A648-dG6P ( Figure 6A), A568-dT6P in conjunction with A648-SAv-dG6P ( Figure 6B), and A568-SAv-Biotin-dT6P in conjunction with A648-SAv-dG6P ( Figure 6C).
- Figure 6A Figure 6A
- Figure 6B shows detection of incorporation of blocks of T and G analogs.
Abstract
La présente invention concerne des compositions d’acide nucléique, des procédés de préparation et d’utilisation de telles compositions qui comprennent des groupes fonctionnels modulaires qui peuvent être configurés pour conférer une fonctionnalité souhaitée à différents types de nucléotide par l’intermédiaire d’un composant de liaison échangeable et de préférence non covalent. De telles compositions sont utiles dans différentes applications comprenant des analyses d’acide nucléique.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11620208P | 2008-11-19 | 2008-11-19 | |
US61/116,202 | 2008-11-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2010059206A2 true WO2010059206A2 (fr) | 2010-05-27 |
WO2010059206A3 WO2010059206A3 (fr) | 2010-08-05 |
Family
ID=42198715
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2009/006177 WO2010059206A2 (fr) | 2008-11-19 | 2009-11-18 | Compositions de nucléotides modulaires et utilisations de celles-ci |
Country Status (2)
Country | Link |
---|---|
US (6) | US8252910B2 (fr) |
WO (1) | WO2010059206A2 (fr) |
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2009
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2012
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2014
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2016
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2017
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2018
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Also Published As
Publication number | Publication date |
---|---|
US20160244824A1 (en) | 2016-08-25 |
US20170159116A1 (en) | 2017-06-08 |
US8846881B2 (en) | 2014-09-30 |
US10161002B2 (en) | 2018-12-25 |
US8252910B2 (en) | 2012-08-28 |
US9551031B2 (en) | 2017-01-24 |
WO2010059206A3 (fr) | 2010-08-05 |
US10745750B2 (en) | 2020-08-18 |
US9879319B2 (en) | 2018-01-30 |
US20180163267A1 (en) | 2018-06-14 |
US20100152424A1 (en) | 2010-06-17 |
US20130022968A1 (en) | 2013-01-24 |
US20190185926A1 (en) | 2019-06-20 |
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