WO2010041736A9 - 表面プラズモンを利用したアッセイ法 - Google Patents

表面プラズモンを利用したアッセイ法 Download PDF

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Publication number
WO2010041736A9
WO2010041736A9 PCT/JP2009/067628 JP2009067628W WO2010041736A9 WO 2010041736 A9 WO2010041736 A9 WO 2010041736A9 JP 2009067628 W JP2009067628 W JP 2009067628W WO 2010041736 A9 WO2010041736 A9 WO 2010041736A9
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WO
WIPO (PCT)
Prior art keywords
assay method
assay
ligand
bringing
sample
Prior art date
Application number
PCT/JP2009/067628
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English (en)
French (fr)
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WO2010041736A1 (ja
Inventor
高敏 彼谷
二宮　英隆
賢治 石田
法明 山本
Original Assignee
コニカミノルタホールディングス株式会社
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Publication date
Priority claimed from JP2008264217A external-priority patent/JP2010091527A/ja
Priority claimed from JP2009025975A external-priority patent/JP5169891B2/ja
Application filed by コニカミノルタホールディングス株式会社 filed Critical コニカミノルタホールディングス株式会社
Publication of WO2010041736A1 publication Critical patent/WO2010041736A1/ja
Publication of WO2010041736A9 publication Critical patent/WO2010041736A9/ja

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

［課題］免疫反応場と検出場とをそれぞれ独立させることによって、高感度かつ高精度であり、イムノアッセイに必要不可欠である特異性に優れた、表面プラズモンを利用したアッセイ法、アッセイ用装置およびアッセイ用キットを提供すること。 ［解決手段］少なくとも下記工程（ａ）～（ｇ）を含むことを特徴とするアッセイ法。　工程（ａ）：リガンドがその表面に固定化された粒子と検体とを接触させる工程、　工程（ｂ）：粒子に、リガンドと酵素とのコンジュゲートを反応させる工程、　工程（ｃ）：さらに基質を反応させる工程、　工程（ｄ）：工程（ｃ）で得られた生成物を単離する工程、　工程（ｅ）：プラズモン励起センサの金属薄膜表面に、生成物を接触させる工程、　工程（ｆ）：プラズモン励起センサにレーザ光を照射し、励起された蛍光色素から発光された蛍光量を測定する工程、および　工程（ｇ）：測定結果から、検体中に含有されるアナライト量を算出する工程。
PCT/JP2009/067628 2008-10-10 2009-10-09 表面プラズモンを利用したアッセイ法 WO2010041736A1 (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2008-264217 2008-10-10
JP2008264217A JP2010091527A (ja) 2008-10-10 2008-10-10 表面プラズモンを利用したアッセイ法
JP2009-025975 2009-02-06
JP2009025975A JP5169891B2 (ja) 2009-02-06 2009-02-06 表面プラズモンを利用したアッセイ法

Publications (2)

Publication Number Publication Date
WO2010041736A1 WO2010041736A1 (ja) 2010-04-15
WO2010041736A9 true WO2010041736A9 (ja) 2010-08-12

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PCT/JP2009/067628 WO2010041736A1 (ja) 2008-10-10 2009-10-09 表面プラズモンを利用したアッセイ法

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Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010125932A1 (ja) * 2009-04-28 2010-11-04 コニカミノルタホールディングス株式会社 融合タンパク質含有集合体、その製造方法及び該集合体を用いたアッセイ法
JP5565125B2 (ja) * 2010-06-16 2014-08-06 コニカミノルタ株式会社 Ｓｐｆｓ（表面プラズモン励起増強蛍光分光法）またはそれを利用した測定方法ならびにそれらの測定方法用の表面プラズモン共鳴センサ
JP5516198B2 (ja) * 2010-07-30 2014-06-11 コニカミノルタ株式会社 プラズモン励起センサチップおよびこれを用いたプラズモン励起センサ、並びにアナライトの検出方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2938485B2 (ja) * 1989-12-25 1999-08-23 株式会社東芝 光学的反応測定法
JP4332056B2 (ja) * 2004-04-01 2009-09-16 Ｏｋｉセミコンダクタ株式会社 半導体集積回路
JP4581128B2 (ja) * 2005-07-20 2010-11-17 独立行政法人産業技術総合研究所 酵素免疫測定方法及びそのための酵素免疫センサ
JP2007240361A (ja) * 2006-03-09 2007-09-20 Sekisui Chem Co Ltd 局在プラズモン増強センサ
JP2008139245A (ja) * 2006-12-05 2008-06-19 Fujifilm Corp 被験物の免疫測定方法

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Publication number Publication date
WO2010041736A1 (ja) 2010-04-15

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WO2010041736A9 (ja) 表面プラズモンを利用したアッセイ法

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