WO2010033753A3 - Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés - Google Patents

Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés Download PDF

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Publication number
WO2010033753A3
WO2010033753A3 PCT/US2009/057393 US2009057393W WO2010033753A3 WO 2010033753 A3 WO2010033753 A3 WO 2010033753A3 US 2009057393 W US2009057393 W US 2009057393W WO 2010033753 A3 WO2010033753 A3 WO 2010033753A3
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WO
WIPO (PCT)
Prior art keywords
mutant
fatty acids
delta5
desaturases
acid
Prior art date
Application number
PCT/US2009/057393
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English (en)
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WO2010033753A2 (fr
Inventor
Qun Zhu
Dana M. Walters Pollak
Original Assignee
E. I. Du Pont De Nemours And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E. I. Du Pont De Nemours And Company filed Critical E. I. Du Pont De Nemours And Company
Priority to EP09815230.9A priority Critical patent/EP2324119B1/fr
Priority to DK09815230.9T priority patent/DK2324119T3/en
Priority to JP2011527976A priority patent/JP5762291B2/ja
Priority to BRPI0913714-9A priority patent/BRPI0913714A2/pt
Priority to CA2735379A priority patent/CA2735379A1/fr
Priority to CN200980136806.0A priority patent/CN102197047B/zh
Priority to AU2009293160A priority patent/AU2009293160B2/en
Publication of WO2010033753A2 publication Critical patent/WO2010033753A2/fr
Publication of WO2010033753A3 publication Critical patent/WO2010033753A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention porte sur des delta5 désaturases mutantes, qui ont l'aptitude de convertir l'acide dihomo-γ-linolénique [DGLA; 20:3 ω-6] en acide arachidonique [ARA; 20:4 ω-6] et/ou l'acide eicosatétraénoïque [ETA; 20:4 ω-3] en acide eicosapentaénoïque [EPA; 20:5 ω-3] et qui possèdent au moins une mutation dans le motif HPGG du domaine de type cytochrome b 5. L'invention porte sur des fragments d'acide nucléique isolés et des produits de construction recombinants comprenant de tels fragments codant pour les delta5 désaturases, conjointement avec un procédé de production d'acides gras polyinsaturés à longue chaîne [« PUFA »] à l'aide de ces delta5 désaturases mutantes dans une levure oléagineuse.
PCT/US2009/057393 2008-09-19 2009-09-18 Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés WO2010033753A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP09815230.9A EP2324119B1 (fr) 2008-09-19 2009-09-18 Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés
DK09815230.9T DK2324119T3 (en) 2008-09-19 2009-09-18 Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
JP2011527976A JP5762291B2 (ja) 2008-09-19 2009-09-18 変異δ５デサチュラーゼと、多価不飽和脂肪酸の製造におけるそれらの使用
BRPI0913714-9A BRPI0913714A2 (pt) 2008-09-19 2009-09-18 polipeptídeo mutante que possui atividade de a5 dessaturase, molécula de ácido nucleico isolada, célula hospedeira microbiana e métodos de produção de ácido araquidônico e de ácido eicosapentaenoico
CA2735379A CA2735379A1 (fr) 2008-09-19 2009-09-18 Delta5 desaturases mutantes et leur utilisation dans la production d'acides gras polyinsatures
CN200980136806.0A CN102197047B (zh) 2008-09-19 2009-09-18 突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途
AU2009293160A AU2009293160B2 (en) 2008-09-19 2009-09-18 Mutant delta5 desaturases and their use in making polyunsaturated fatty acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9833308P 2008-09-19 2008-09-19
US61/098,333 2008-09-19

Publications (2)

Publication Number Publication Date
WO2010033753A2 WO2010033753A2 (fr) 2010-03-25
WO2010033753A3 true WO2010033753A3 (fr) 2010-05-27

Family

ID=42038064

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/057393 WO2010033753A2 (fr) 2008-09-19 2009-09-18 Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés

Country Status (10)

Country Link
US (3) US8268598B2 (fr)
EP (1) EP2324119B1 (fr)
JP (1) JP5762291B2 (fr)
CN (1) CN102197047B (fr)
AU (1) AU2009293160B2 (fr)
BR (1) BRPI0913714A2 (fr)
CA (1) CA2735379A1 (fr)
CL (1) CL2011000560A1 (fr)
DK (1) DK2324119T3 (fr)
WO (1) WO2010033753A2 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2324119T3 (en) * 2008-09-19 2016-07-18 Du Pont Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
WO2011133610A1 (fr) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Procédé d'obtention de compositions contenant des acides gras poly-insaturés à partir de biomasse microbienne
EP2580229A4 (fr) * 2010-06-10 2013-12-25 Univ Ben Gurion Gène mutant défectueux pauvre en désaturase delta 5 et utilisation de ce dernier
WO2012027689A1 (fr) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Cellules hôtes microbiennes recombinées pour la production élevée d'acide eicosapentaénoïque
CN103080316B (zh) 2010-08-26 2016-08-31 纳幕尔杜邦公司 突变δ9延伸酶和它们在制备多不饱和脂肪酸中的用途
EP2609202A1 (fr) * 2010-08-26 2013-07-03 E.I. Du Pont De Nemours And Company Delta-5 désaturases à motif hpgg et à motif hdash mutés et leur utilisation dans la fabrication d'acides gras polyinsaturés
JP2014503216A (ja) 2010-12-30 2014-02-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー スクロース利用のための、ヤロウィア・リポリティカ（Ｙａｒｒｏｗｉａｌｉｐｏｌｙｔｉｃａ）におけるサッカロミセス・セレビシア（Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）ＳＵＣ２遺伝子の使用
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CA2825039C (fr) 2011-02-11 2020-04-28 E.I. Du Pont De Nemours And Company Procede d'obtention de composition contenant des lipides a partir de biomasse microbienne
BR112014031827A2 (pt) * 2012-06-19 2017-06-27 Du Pont produção aprimorada de ácidos graxos poli-insaturados por coexpressão de acil- coa: lisofosfatidilcolina aciltransferases e fosfolipídeo: diacilglicerol aciltransferases.
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
WO2014074772A1 (fr) 2012-11-09 2014-05-15 Heliae Development, Llc Procédés et systèmes de combinaisons de mixotrophes, phototrophes et hétérotrophes
WO2014074770A2 (fr) 2012-11-09 2014-05-15 Heliae Development, Llc Procédés à mixotrophie équilibrée
JP2016502851A (ja) 2012-12-21 2016-02-01 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 微生物細胞中における脂質生成を改変するためのｓｏｕ２ソルビトール利用タンパク質をコードするポリヌクレオチドの下方制御
WO2014133159A1 (fr) * 2013-02-28 2014-09-04 株式会社ユーグレナ Procédé d'introduction d'un gène chez l'euglène, et transformant ainsi obtenu
JP2014193153A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナへの遺伝子導入方法
JP2014193154A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナの形質転換体
EP4049537A1 (fr) 2018-12-12 2022-08-31 Nippon Suisan Kaisha, Ltd. Composition contenant un acide gras hautement insaturé ou un ester d'alkyle et son procédé de production

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070271632A1 (en) * 2006-05-17 2007-11-22 Howard Glenn Damude Delta 5 desaturase and its use in making polyunsaturated fatty acids
US20080155705A1 (en) * 2003-08-01 2008-06-26 Basf Plant Science Gmbh Method For the Production of Multiple-Unsaturated Fatty Acids in Transgenic Organisms

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US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
US8323935B2 (en) 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
DK2324119T3 (en) * 2008-09-19 2016-07-18 Du Pont Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080155705A1 (en) * 2003-08-01 2008-06-26 Basf Plant Science Gmbh Method For the Production of Multiple-Unsaturated Fatty Acids in Transgenic Organisms
US20070271632A1 (en) * 2006-05-17 2007-11-22 Howard Glenn Damude Delta 5 desaturase and its use in making polyunsaturated fatty acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SAYANOVA ET AL.: "The alternative pathway C20 D8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5-fusion desaturase", FEBS LETTERS, vol. 580, 2006, pages 1946 - 1952, XP025170961 *

Also Published As

Publication number Publication date
EP2324119A2 (fr) 2011-05-25
EP2324119B1 (fr) 2016-03-30
DK2324119T3 (en) 2016-07-18
BRPI0913714A2 (pt) 2020-10-13
US8268598B2 (en) 2012-09-18
US8614075B2 (en) 2013-12-24
AU2009293160B2 (en) 2013-11-21
JP2012502659A (ja) 2012-02-02
US20100075386A1 (en) 2010-03-25
US8367383B2 (en) 2013-02-05
CA2735379A1 (fr) 2010-03-25
US20120309064A1 (en) 2012-12-06
US20130122558A1 (en) 2013-05-16
AU2009293160A1 (en) 2010-03-25
JP5762291B2 (ja) 2015-08-12
CN102197047A (zh) 2011-09-21
CN102197047B (zh) 2014-07-09
EP2324119A4 (fr) 2013-05-01
WO2010033753A2 (fr) 2010-03-25
CL2011000560A1 (es) 2011-10-07

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