WO2010033753A3 - Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés - Google Patents

Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés Download PDF

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Publication number
WO2010033753A3
WO2010033753A3 PCT/US2009/057393 US2009057393W WO2010033753A3 WO 2010033753 A3 WO2010033753 A3 WO 2010033753A3 US 2009057393 W US2009057393 W US 2009057393W WO 2010033753 A3 WO2010033753 A3 WO 2010033753A3
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WO
WIPO (PCT)
Prior art keywords
mutant
fatty acids
delta5
desaturases
acid
Prior art date
Application number
PCT/US2009/057393
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English (en)
Other versions
WO2010033753A2 (fr
Inventor
Qun Zhu
Dana M. Walters Pollak
Original Assignee
E. I. Du Pont De Nemours And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E. I. Du Pont De Nemours And Company filed Critical E. I. Du Pont De Nemours And Company
Priority to EP09815230.9A priority Critical patent/EP2324119B1/fr
Priority to JP2011527976A priority patent/JP5762291B2/ja
Priority to DK09815230.9T priority patent/DK2324119T3/en
Priority to BRPI0913714-9A priority patent/BRPI0913714A2/pt
Priority to CA2735379A priority patent/CA2735379A1/fr
Priority to AU2009293160A priority patent/AU2009293160B2/en
Priority to CN200980136806.0A priority patent/CN102197047B/zh
Publication of WO2010033753A2 publication Critical patent/WO2010033753A2/fr
Publication of WO2010033753A3 publication Critical patent/WO2010033753A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention porte sur des delta5 désaturases mutantes, qui ont l'aptitude de convertir l'acide dihomo-γ-linolénique [DGLA; 20:3 ω-6] en acide arachidonique [ARA; 20:4 ω-6] et/ou l'acide eicosatétraénoïque [ETA; 20:4 ω-3] en acide eicosapentaénoïque [EPA; 20:5 ω-3] et qui possèdent au moins une mutation dans le motif HPGG du domaine de type cytochrome b 5. L'invention porte sur des fragments d'acide nucléique isolés et des produits de construction recombinants comprenant de tels fragments codant pour les delta5 désaturases, conjointement avec un procédé de production d'acides gras polyinsaturés à longue chaîne [« PUFA »] à l'aide de ces delta5 désaturases mutantes dans une levure oléagineuse.
PCT/US2009/057393 2008-09-19 2009-09-18 Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés WO2010033753A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP09815230.9A EP2324119B1 (fr) 2008-09-19 2009-09-18 Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés
JP2011527976A JP5762291B2 (ja) 2008-09-19 2009-09-18 変異δ５デサチュラーゼと、多価不飽和脂肪酸の製造におけるそれらの使用
DK09815230.9T DK2324119T3 (en) 2008-09-19 2009-09-18 Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
BRPI0913714-9A BRPI0913714A2 (pt) 2008-09-19 2009-09-18 polipeptídeo mutante que possui atividade de a5 dessaturase, molécula de ácido nucleico isolada, célula hospedeira microbiana e métodos de produção de ácido araquidônico e de ácido eicosapentaenoico
CA2735379A CA2735379A1 (fr) 2008-09-19 2009-09-18 Delta5 desaturases mutantes et leur utilisation dans la production d'acides gras polyinsatures
AU2009293160A AU2009293160B2 (en) 2008-09-19 2009-09-18 Mutant delta5 desaturases and their use in making polyunsaturated fatty acids
CN200980136806.0A CN102197047B (zh) 2008-09-19 2009-09-18 突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9833308P 2008-09-19 2008-09-19
US61/098,333 2008-09-19

Publications (2)

Publication Number Publication Date
WO2010033753A2 WO2010033753A2 (fr) 2010-03-25
WO2010033753A3 true WO2010033753A3 (fr) 2010-05-27

Family

ID=42038064

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/057393 WO2010033753A2 (fr) 2008-09-19 2009-09-18 Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés

Country Status (10)

Country Link
US (3) US8268598B2 (fr)
EP (1) EP2324119B1 (fr)
JP (1) JP5762291B2 (fr)
CN (1) CN102197047B (fr)
AU (1) AU2009293160B2 (fr)
BR (1) BRPI0913714A2 (fr)
CA (1) CA2735379A1 (fr)
CL (1) CL2011000560A1 (fr)
DK (1) DK2324119T3 (fr)
WO (1) WO2010033753A2 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010033753A2 (fr) * 2008-09-19 2010-03-25 E. I. Du Pont De Nemours And Company Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés
CN103282474A (zh) 2010-04-22 2013-09-04 纳幕尔杜邦公司 从微生物生物质中获得包含多不饱和脂肪酸的组合物的方法
US20140093910A1 (en) * 2010-06-10 2014-04-03 Bengurion University Of The Negev Research And Development Authority D5 desaturase-defective mutant gene and use thereof
BR112013004356A2 (pt) 2010-08-26 2017-06-27 Du Pont polinucleotídeo isolado, polipeptídeo mutante, constructo recombinante, célula transformada, método para produção de um ácido graxo poli-insaturado, óleo microbiano e célula hospedeira microbiana recombinante.
WO2012027698A1 (fr) 2010-08-26 2012-03-01 E.I. Du Pont De Nemours And Company Delta-5 désaturases à motif hpgg et à motif hdash mutés et leur utilisation dans la fabrication d'acides gras polyinsaturés
AU2011293180B2 (en) 2010-08-26 2017-03-02 E. I. Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
KR20140000711A (ko) 2010-12-30 2014-01-03 이 아이 듀폰 디 네모아 앤드 캄파니 수크로스 이용에 있어서의 야로위아 리포라이티카에서의 사카로마이세스 세레비지애 suc2 유전자의 용도
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
US8999663B2 (en) 2011-02-11 2015-04-07 E L Du Pont De Nemours And Company Method for obtaining a lipid-containing composition from microbial biomass
EP2861728B1 (fr) * 2012-06-19 2017-12-27 E. I. du Pont de Nemours and Company Production améliorée d'acides gras polyinsaturés par coexpression d'acyl-coa: lysophosphatidylcholine acyltransférases et de phospholipide: diacylglycérol acyltransférases
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
WO2014074770A2 (fr) 2012-11-09 2014-05-15 Heliae Development, Llc Procédés à mixotrophie équilibrée
WO2014074772A1 (fr) 2012-11-09 2014-05-15 Heliae Development, Llc Procédés et systèmes de combinaisons de mixotrophes, phototrophes et hétérotrophes
EP2935591B1 (fr) 2012-12-21 2017-07-05 E. I. du Pont de Nemours and Company Régulation à la baisse d'un polynucléotide codant pour une protéine d'utilisation du sorbitol sou2 pour modifier la production de lipides dans des cellules microbiennes
US20160010070A1 (en) * 2013-02-28 2016-01-14 Kinki University Method for introducing gene to euglena, and transformant therefrom
JP2014193153A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナへの遺伝子導入方法
JP2014193154A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナの形質転換体
EP4049537A1 (fr) 2018-12-12 2022-08-31 Nippon Suisan Kaisha, Ltd. Composition contenant un acide gras hautement insaturé ou un ester d'alkyle et son procédé de production

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070271632A1 (en) * 2006-05-17 2007-11-22 Howard Glenn Damude Delta 5 desaturase and its use in making polyunsaturated fatty acids
US20080155705A1 (en) * 2003-08-01 2008-06-26 Basf Plant Science Gmbh Method For the Production of Multiple-Unsaturated Fatty Acids in Transgenic Organisms

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
WO2009046231A1 (fr) * 2007-10-03 2009-04-09 E. I. Du Pont De Nemours And Company Souches optimisées de yarrowia lipolytica pour une production élevée d'acide eicosapentaénoïque
WO2010033753A2 (fr) * 2008-09-19 2010-03-25 E. I. Du Pont De Nemours And Company Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080155705A1 (en) * 2003-08-01 2008-06-26 Basf Plant Science Gmbh Method For the Production of Multiple-Unsaturated Fatty Acids in Transgenic Organisms
US20070271632A1 (en) * 2006-05-17 2007-11-22 Howard Glenn Damude Delta 5 desaturase and its use in making polyunsaturated fatty acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SAYANOVA ET AL.: "The alternative pathway C20 D8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5-fusion desaturase", FEBS LETTERS, vol. 580, 2006, pages 1946 - 1952, XP025170961 *

Also Published As

Publication number Publication date
US8367383B2 (en) 2013-02-05
EP2324119A2 (fr) 2011-05-25
AU2009293160B2 (en) 2013-11-21
BRPI0913714A2 (pt) 2020-10-13
DK2324119T3 (en) 2016-07-18
JP2012502659A (ja) 2012-02-02
EP2324119A4 (fr) 2013-05-01
CA2735379A1 (fr) 2010-03-25
US20100075386A1 (en) 2010-03-25
US8614075B2 (en) 2013-12-24
US20130122558A1 (en) 2013-05-16
US20120309064A1 (en) 2012-12-06
US8268598B2 (en) 2012-09-18
EP2324119B1 (fr) 2016-03-30
JP5762291B2 (ja) 2015-08-12
CN102197047B (zh) 2014-07-09
WO2010033753A2 (fr) 2010-03-25
CN102197047A (zh) 2011-09-21
CL2011000560A1 (es) 2011-10-07
AU2009293160A1 (en) 2010-03-25

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