WO2008000277A8 - Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés - Google Patents
Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés Download PDFInfo
- Publication number
- WO2008000277A8 WO2008000277A8 PCT/DK2007/050079 DK2007050079W WO2008000277A8 WO 2008000277 A8 WO2008000277 A8 WO 2008000277A8 DK 2007050079 W DK2007050079 W DK 2007050079W WO 2008000277 A8 WO2008000277 A8 WO 2008000277A8
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- fatty acids
- cytochrome
- fungal cells
- polyunsaturated fatty
- increased content
- Prior art date
Links
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 title abstract 4
- 230000002538 fungal effect Effects 0.000 title abstract 2
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 abstract 6
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 abstract 3
- 229940114079 arachidonic acid Drugs 0.000 abstract 3
- 235000021342 arachidonic acid Nutrition 0.000 abstract 3
- 229960005135 eicosapentaenoic acid Drugs 0.000 abstract 3
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 abstract 3
- 235000020673 eicosapentaenoic acid Nutrition 0.000 abstract 3
- 102100033149 Cytochrome b5 reductase 4 Human genes 0.000 abstract 2
- 108030005700 Cytochrome-b5 reductases Proteins 0.000 abstract 2
- 102000007605 Cytochromes b5 Human genes 0.000 abstract 2
- 108010007167 Cytochromes b5 Proteins 0.000 abstract 2
- 235000014113 dietary fatty acids Nutrition 0.000 abstract 2
- 229930195729 fatty acid Natural products 0.000 abstract 2
- 239000000194 fatty acid Substances 0.000 abstract 2
- 150000004665 fatty acids Chemical class 0.000 abstract 2
- 230000014509 gene expression Effects 0.000 abstract 2
- 102000015303 Fatty Acid Synthases Human genes 0.000 abstract 1
- 108010039731 Fatty Acid Synthases Proteins 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 1
- 230000002018 overexpression Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
- C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
- C12N9/004—Cytochrome-b5 reductase (1.6.2.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
- C12P7/6432—Eicosapentaenoic acids [EPA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention concerne la production d'acides gras et, plus particulièrement, la production d'acide arachidonique (ARA) et d'acide eicosapentanoïque (EPA) d'acides gras polyinsaturés (PUFA) dans des cellules fongiques génétiquement modifiées, notamment, des cellules de Saccharomyces cerevisiae métaboliquement modifiées avec une teneur élevée en ARA et en EPA. L'invention se rapporte particulièrement à l'accroissement de la teneur en PUFA dans l'organisme hôte au moyen, par exemple, de diverses sur-expressions du cytochrome b5 et de la réductase du cytochrome b5 impliquées dans la désaturation d'acide gras, et de l'expression hétérologue du cytochrome b5 et de la réductase de cytochrome b5 ainsi que de l'expression de synthases d'acides gras hétérologues.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US81764306P | 2006-06-29 | 2006-06-29 | |
US60/817,643 | 2006-06-29 |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2008000277A2 WO2008000277A2 (fr) | 2008-01-03 |
WO2008000277A3 WO2008000277A3 (fr) | 2008-03-20 |
WO2008000277A8 true WO2008000277A8 (fr) | 2009-07-30 |
Family
ID=37188928
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK2007/050079 WO2008000277A2 (fr) | 2006-06-29 | 2007-06-29 | Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2008000277A2 (fr) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0725075D0 (en) * | 2007-12-21 | 2008-01-30 | Fluxome Sciences As | Metabolically engineered fungal cells with increased content of polyunsaturated fatty acids |
EP2108702A1 (fr) * | 2008-04-07 | 2009-10-14 | Organo Balance GmbH | Synthèse d'acides gras polyinsaturés dans la levure |
FR2940315B1 (fr) * | 2008-12-19 | 2012-12-14 | Airbus France | Nouveau procede de culture de levures du genre yarrowia |
EP3075848A1 (fr) * | 2015-04-01 | 2016-10-05 | Johann Wolfgang Goethe-Universität Frankfurt am Main | Production microbiologique d'acides gras courts et leurs utilisations |
KR101957910B1 (ko) | 2017-05-02 | 2019-03-15 | 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 | 효모의 세포 소기관(organelle) 개량을 통한 진세노사이드 생산 증대 |
WO2021018783A1 (fr) * | 2019-07-26 | 2021-02-04 | Novozymes A/S | Cellule hôte fongique filamenteuse modifiée |
JP2024521047A (ja) * | 2021-05-10 | 2024-05-28 | エフエムシー アグリカルチュラル ソリューションズ エー/エス | 酵素活性の増加及び昆虫フェロモンの生成のための改善された方法及び細胞 |
CN114478727A (zh) * | 2022-01-17 | 2022-05-13 | 华中科技大学 | 高山被孢霉油脂合成相关转录因子ino4及其编码基因与应用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6448055B1 (en) * | 1997-02-27 | 2002-09-10 | Suntory Limited | Δ9-desaturase gene |
PL1766023T3 (pl) * | 2004-06-04 | 2011-04-29 | Fluxome Sciences As | Metabolicznie zmodyfikowane komórki do wytwarzania wielonienasyconych kwasów tłuszczowych |
-
2007
- 2007-06-29 WO PCT/DK2007/050079 patent/WO2008000277A2/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2008000277A2 (fr) | 2008-01-03 |
WO2008000277A3 (fr) | 2008-03-20 |
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