WO2008000277A8 - Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés - Google Patents

Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés Download PDF

Info

Publication number
WO2008000277A8
WO2008000277A8 PCT/DK2007/050079 DK2007050079W WO2008000277A8 WO 2008000277 A8 WO2008000277 A8 WO 2008000277A8 DK 2007050079 W DK2007050079 W DK 2007050079W WO 2008000277 A8 WO2008000277 A8 WO 2008000277A8
Authority
WO
WIPO (PCT)
Prior art keywords
fatty acids
cytochrome
fungal cells
polyunsaturated fatty
increased content
Prior art date
Application number
PCT/DK2007/050079
Other languages
English (en)
Other versions
WO2008000277A3 (fr
WO2008000277A2 (fr
Inventor
Jochen Foerster
Nina Katarina Gunnarsson
Malin Inga Elisabeth Svensson
Helga Susana Moreira David
Iben Plate
Sabina De Andrade Pereira Tavares
Jean-Marie Mouillion
Sara Petersen Bjoern
Per Moeller
Original Assignee
Fluxome Sciences As
Jochen Foerster
Nina Katarina Gunnarsson
Malin Inga Elisabeth Svensson
Helga Susana Moreira David
Iben Plate
Tavares Sabina De Andrade Pere
Jean-Marie Mouillion
Sara Petersen Bjoern
Per Moeller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluxome Sciences As, Jochen Foerster, Nina Katarina Gunnarsson, Malin Inga Elisabeth Svensson, Helga Susana Moreira David, Iben Plate, Tavares Sabina De Andrade Pere, Jean-Marie Mouillion, Sara Petersen Bjoern, Per Moeller filed Critical Fluxome Sciences As
Publication of WO2008000277A2 publication Critical patent/WO2008000277A2/fr
Publication of WO2008000277A3 publication Critical patent/WO2008000277A3/fr
Publication of WO2008000277A8 publication Critical patent/WO2008000277A8/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/004—Cytochrome-b5 reductase (1.6.2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne la production d'acides gras et, plus particulièrement, la production d'acide arachidonique (ARA) et d'acide eicosapentanoïque (EPA) d'acides gras polyinsaturés (PUFA) dans des cellules fongiques génétiquement modifiées, notamment, des cellules de Saccharomyces cerevisiae métaboliquement modifiées avec une teneur élevée en ARA et en EPA. L'invention se rapporte particulièrement à l'accroissement de la teneur en PUFA dans l'organisme hôte au moyen, par exemple, de diverses sur-expressions du cytochrome b5 et de la réductase du cytochrome b5 impliquées dans la désaturation d'acide gras, et de l'expression hétérologue du cytochrome b5 et de la réductase de cytochrome b5 ainsi que de l'expression de synthases d'acides gras hétérologues.
PCT/DK2007/050079 2006-06-29 2007-06-29 Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés WO2008000277A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81764306P 2006-06-29 2006-06-29
US60/817,643 2006-06-29

Publications (3)

Publication Number Publication Date
WO2008000277A2 WO2008000277A2 (fr) 2008-01-03
WO2008000277A3 WO2008000277A3 (fr) 2008-03-20
WO2008000277A8 true WO2008000277A8 (fr) 2009-07-30

Family

ID=37188928

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2007/050079 WO2008000277A2 (fr) 2006-06-29 2007-06-29 Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés

Country Status (1)

Country Link
WO (1) WO2008000277A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0725075D0 (en) * 2007-12-21 2008-01-30 Fluxome Sciences As Metabolically engineered fungal cells with increased content of polyunsaturated fatty acids
EP2108702A1 (fr) * 2008-04-07 2009-10-14 Organo Balance GmbH Synthèse d'acides gras polyinsaturés dans la levure
FR2940315B1 (fr) * 2008-12-19 2012-12-14 Airbus France Nouveau procede de culture de levures du genre yarrowia
EP3075848A1 (fr) 2015-04-01 2016-10-05 Johann Wolfgang Goethe-Universität Frankfurt am Main Production microbiologique d'acides gras courts et leurs utilisations
KR101957910B1 (ko) 2017-05-02 2019-03-15 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 효모의 세포 소기관(organelle) 개량을 통한 진세노사이드 생산 증대
EP4004187A1 (fr) * 2019-07-26 2022-06-01 Novozymes A/S Cellule hôte fongique filamenteuse modifiée
JP2024521047A (ja) * 2021-05-10 2024-05-28 エフエムシー アグリカルチュラル ソリューションズ エー／エス 酵素活性の増加及び昆虫フェロモンの生成のための改善された方法及び細胞
CN114478727A (zh) * 2022-01-17 2022-05-13 华中科技大学 高山被孢霉油脂合成相关转录因子ino4及其编码基因与应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE435288T1 (de) * 1997-02-27 2009-07-15 Suntory Holdings Ltd Delta-9 desaturase gen
DE602005023308D1 (de) * 2004-06-04 2010-10-14 Fluxome Sciences As Stoffwechseltechnisch hergestellte zellen zur produktion mehrfach ungesättigter fettsäuren

Also Published As

Publication number Publication date
WO2008000277A3 (fr) 2008-03-20
WO2008000277A2 (fr) 2008-01-03

Similar Documents

Publication Publication Date Title
WO2008000277A8 (fr) Cellules fongiques métaboliquement modifiées à teneur élevée en acides gras polyinsaturés
EP2392665A3 (fr) Production d'acides gras polyinsaturés dans des levures oléagineuses
WO2007136671A3 (fr) Delta5 désaturase et son utilisation dans la préparation d'acides gras polyinsaturés
WO2006052870A3 (fr) Souches produisant de l'acide eicosapentanoique de yarrowia lipolytica
WO2005003310A3 (fr) Promoteurs de la glyceraldehyde-3-phosphate dehydrogenase et de la phosphoglycerate mutase pour l'expression genique dans de la levure oleagineuse
WO2005047485A3 (fr) Desaturases $g(d)12 pouvant etre utilisees pour modifier les niveaux d'acides gras polyinsatures dans une levure oleagineuse
WO2004101753A3 (fr) Genes a condons optimises pour la production d'acides grs polyinsatures dans des levures oleagineuses
WO2005047479A3 (fr) $g(d)-15 desaturases appropriees pour modifier les niveaux d'acides gras polyinsatures dans des plantes a graines oleagineuses et de la levure oleagineuse
WO2010129565A3 (fr) Compositions, procédés et trousses pour des acides gras polyinsaturés provenant de microalgues
NZ593097A (en) Enzymes and methods for producing omega-3 fatty acids
WO2005118814A3 (fr) Cellules metaboliquement transformees pour la production d'acides gras polyinsaturates
EP2363492A3 (fr) Synthèse d'acides gras polyinsaturés à longue chaîne par cellules recombinantes
WO2003064596A3 (fr) Genes de desaturase, enzymes codes par ces genes et utilisation de ceux-ci
WO2006031937A3 (fr) Promoteur de glycerol-3-phosphate o-acyltransferase pour l'expression genique dans une levure oleagineuse
WO2010033753A3 (fr) Delta5 désaturases mutantes et leur utilisation dans la production d'acides gras polyinsaturés
WO2007123999A3 (fr) Delta-17-desaturases et leur utilisation dans la preparation d'acides gras polyinsatures
WO2010066703A3 (fr) Désaturases et procédé pour la production d'acides gras polyinsaturés dans des organismes transgéniques
WO2012052468A3 (fr) Nouvelles désaturases d'acides gras, élongases, composants d'allongement leurs utilisations
EP1698685A4 (fr) Composition a base de graisses
WO2006081644A3 (fr) Procede catalytique d'esterification d'acides gras
WO2007133425A3 (fr) Motifs de désaturase delta12 et de désaturase delta15 fongiques
Zhuang et al. Key enzymes in fatty acid synthesis pathway for bioactive lipids biosynthesis
WO2009114830A3 (fr) Procédés et compositions pour extraction et transestérification de composants de biomasse
HK1163655A1 (en) Polyunsaturated fatty acid and diol ester as an anti-acne agent
WO2009124694A3 (fr) Synthèse d'acides gras polyinsaturés dans une levure

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07764499

Country of ref document: EP

Kind code of ref document: A2

DPE1 Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101)
NENP Non-entry into the national phase

Ref country code: DE

NENP Non-entry into the national phase

Ref country code: RU

122 Ep: pct application non-entry in european phase

Ref document number: 07764499

Country of ref document: EP

Kind code of ref document: A2