WO2010019006A3 - 시스토비루스 phi6의 외피막 단백질 P9을 융합파트너로 포함하는 발현벡터 및 이를 이용한 막 단백질의 생산방법 - Google Patents

시스토비루스 phi6의 외피막 단백질 P9을 융합파트너로 포함하는 발현벡터 및 이를 이용한 막 단백질의 생산방법 Download PDF

Info

Publication number
WO2010019006A3
WO2010019006A3 PCT/KR2009/004525 KR2009004525W WO2010019006A3 WO 2010019006 A3 WO2010019006 A3 WO 2010019006A3 KR 2009004525 W KR2009004525 W KR 2009004525W WO 2010019006 A3 WO2010019006 A3 WO 2010019006A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression vector
producing
vector containing
protein
same
Prior art date
Application number
PCT/KR2009/004525
Other languages
English (en)
French (fr)
Other versions
WO2010019006A2 (ko
Inventor
임동빈
정윤아
Original Assignee
숭실대학교산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 숭실대학교산학협력단 filed Critical 숭실대학교산학협력단
Priority to US13/058,425 priority Critical patent/US8628939B2/en
Publication of WO2010019006A2 publication Critical patent/WO2010019006A2/ko
Publication of WO2010019006A3 publication Critical patent/WO2010019006A3/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/12011—Details dsRNA Bacteriophages
    • C12N2795/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 시스토비루스 phi6의 외피막 단백질 (major envelope protein) P9을 융합파트너로 포함하는 막 단백질 발현벡터, 이 발현벡터로 형질전환된 세포 및 이 세포를 이용하여 막 단백질을 생산하는 방법에 관한 것으로, 본 발명에 따른 발현벡터를 이용하면 목적 막 단백질을 효과적으로 발현시킬 수 있다.
PCT/KR2009/004525 2008-08-13 2009-08-13 시스토비루스 phi6의 외피막 단백질 P9을 융합파트너로 포함하는 발현벡터 및 이를 이용한 막 단백질의 생산방법 WO2010019006A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/058,425 US8628939B2 (en) 2008-08-13 2009-08-13 Expression vector containing the major envelope protein P9 of cystovirus PHI6 as a fusion partner, and process for producing a membrane protein using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0079542 2008-08-13
KR1020080079542A KR101443920B1 (ko) 2008-08-13 2008-08-13 시스토비루스 ｐｈｉ6의 외피막 단백질 Ｐ9을 융합파트너로포함하는 막 단백질 발현벡터 및 이를 이용한 막 단백질의생산방법

Publications (2)

Publication Number Publication Date
WO2010019006A2 WO2010019006A2 (ko) 2010-02-18
WO2010019006A3 true WO2010019006A3 (ko) 2010-06-24

Family

ID=41669495

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/004525 WO2010019006A2 (ko) 2008-08-13 2009-08-13 시스토비루스 phi6의 외피막 단백질 P9을 융합파트너로 포함하는 발현벡터 및 이를 이용한 막 단백질의 생산방법

Country Status (3)

Country Link
US (1) US8628939B2 (ko)
KR (1) KR101443920B1 (ko)
WO (1) WO2010019006A2 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101505697B1 (ko) * 2010-11-12 2015-03-30 숭실대학교산학협력단 시스토바이러스 파이12의 외피막 단백질 피9을 융합파트너로 포함하는 막 단백질 발현벡터 및 이를 이용한 막 단백질 제조 방법
KR101912631B1 (ko) * 2015-12-03 2018-10-30 국민대학교산학협력단 막 단백질 제조용 융합 단백질, 발현 벡터, 및 이를 이용한 막 단백질 제조방법
KR102137660B1 (ko) 2018-07-23 2020-07-24 강원대학교산학협력단 Cd1 단백질의 생산 및 정제 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006069331A2 (en) * 2004-12-22 2006-06-29 The Salk Institute For Biological Studies Compositions and methods for producing recombinant proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006069331A2 (en) * 2004-12-22 2006-06-29 The Salk Institute For Biological Studies Compositions and methods for producing recombinant proteins

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DREW, D. ET AL.: "Assembly and overexpression of membrane proteins in Escherichia coli", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1610, 2003, pages 3 - 10 *
NELLI J STROMSTEN ET AL.: "In vitro DNA Packaging of PRD1: A Common Mechanism for Internal-membrane Viruses", J MOL BIOL., vol. 348, 2005, pages 617 - 629 *
NELLI KARHU (NEE STROMSTEN): "The genome packaging machinery of dsDNA bacteriophage PRD1", THESIS DISSERTATION, INSTITUTE OF BIOTECHNOLOGY AND DEPARTMENT OF BIOLOGICAL AND ENVIRONMENTAL SCIENCES DIVISION OF GENETICS AND VIIKKI GRADUATE SCHOOL IN BIOSCIENCES UNIVERSITY OF HELSINKI, 2006 *
ROOSILD, T.P. ET AL.: "Characterization of the family of Mistic homologues", BMC STRUCTURAL BIOLOGY, vol. 6, no. 10, May 2006 (2006-05-01), pages 1 - 6 *
ROOSILD, T.P. ET AL.: "NMR structure of Mistic, a membrane-integrating protein for membrane protein expression", SCIENCE, vol. 307, 2005, pages 1317 - 1321 *
ZUO, X. ET AL.: "Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli", J STRUCT FUNCT GENOMICS, vol. 6, 2005, pages 103 - 111 *

Also Published As

Publication number Publication date
WO2010019006A2 (ko) 2010-02-18
US8628939B2 (en) 2014-01-14
KR101443920B1 (ko) 2014-10-01
US20110287484A1 (en) 2011-11-24
KR20100020784A (ko) 2010-02-23

Similar Documents

Publication Publication Date Title
WO2009149956A3 (en) Fusion protein and use thereof
WO2012045082A3 (en) Engineered nucleic acids and methods of use thereof
WO2012158818A3 (en) Multi-specific fab fusion proteins and methods of use
WO2010120514A3 (en) Antigen-binding proteins comprising recombinant protein scaffolds
WO2009114702A3 (en) Recombinant production of authentic human proteins using human cell expression systems
WO2008154014A3 (en) A method for culturing mammalian cells to improve recombinant protein production
WO2012139134A3 (en) Methods of modulating oncogenic fusion proteins
WO2010096588A3 (en) Cell culture media containing combinations of proteins
AU2009209251A8 (en) Stabilized Angiopoietin-2 antibodies and uses thereof
KR20180084680A (ko) 식물 세포에서의 목적 단백질 발현을 위한 재조합 벡터
SG10201810830UA (en) Cell culture compositions and methods for polypeptide production
WO2012142591A3 (en) Compositions, methods and uses for multiplex protein sequence activity relationship mapping
WO2012054929A3 (en) Use of human serum albumin to decrease antigenicity of therapeutic proteins
WO2008104890A3 (en) Compositions and methods for producing apolipoprotein
MX2010007716A (es) Combinacion de una proteina de fusion del anticuerpo-il-2 de anti-edb fibronectina y una molecula que se une a celulas b, progenitores de la celula b y/o sus contrapartes cancerigenas.
WO2011129462A3 (en) A YEAST EXTRACT CONTAINING γ-Glu-X OR γ-Glu-X-Gly AND A METHOD FOR PRODUCING THE SAME
WO2011153276A3 (en) Stable, functional chimeric cellobiohydrolase class i enzymes
WO2008114673A1 (ja) ポリペプチドの製造方法
MX356454B (es) Una formulacion enzimatica liquida y un proceso para su preparacion.
WO2009137778A3 (en) Methods and compositions for genetically engineering clostridia species
WO2011098999A3 (en) Chemical preparation of ubiquitin thioesters and modifications thereof
WO2009158627A3 (en) Cellulosic protein expression in yeast
IN2015DN03206A (ko)
WO2008033556A3 (en) High pressure treatment of proteins for reduced immunogenicity
WO2013103246A3 (ko) 퀴놀린산을 생산하는 재조합 미생물 및 이를 이용한 퀴놀린산의 생산 방법

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09806868

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 13058425

Country of ref document: US

122 Ep: pct application non-entry in european phase

Ref document number: 09806868

Country of ref document: EP

Kind code of ref document: A2