WO2010019006A2 - 시스토비루스 phi6의 외피막 단백질 P9을 융합파트너로 포함하는 발현벡터 및 이를 이용한 막 단백질의 생산방법 - Google Patents

시스토비루스 phi6의 외피막 단백질 P9을 융합파트너로 포함하는 발현벡터 및 이를 이용한 막 단백질의 생산방법 Download PDF

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WO2010019006A2
WO2010019006A2 PCT/KR2009/004525 KR2009004525W WO2010019006A2 WO 2010019006 A2 WO2010019006 A2 WO 2010019006A2 KR 2009004525 W KR2009004525 W KR 2009004525W WO 2010019006 A2 WO2010019006 A2 WO 2010019006A2
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expression vector
producing
vector containing
protein
same
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PCT/KR2009/004525
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French (fr)
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WO2010019006A3 (ko
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임동빈
정윤아
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숭실대학교산학협력단
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Publication of WO2010019006A2 publication Critical patent/WO2010019006A2/ko
Publication of WO2010019006A3 publication Critical patent/WO2010019006A3/ko

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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
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    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/12011—Details dsRNA Bacteriophages
    • C12N2795/12022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract

본 발명은 시스토비루스 phi6의 외피막 단백질 (major envelope protein) P9을 융합파트너로 포함하는 막 단백질 발현벡터, 이 발현벡터로 형질전환된 세포 및 이 세포를 이용하여 막 단백질을 생산하는 방법에 관한 것으로, 본 발명에 따른 발현벡터를 이용하면 목적 막 단백질을 효과적으로 발현시킬 수 있다.
PCT/KR2009/004525 2008-08-13 2009-08-13 시스토비루스 phi6의 외피막 단백질 P9을 융합파트너로 포함하는 발현벡터 및 이를 이용한 막 단백질의 생산방법 WO2010019006A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/058,425 US8628939B2 (en) 2008-08-13 2009-08-13 Expression vector containing the major envelope protein P9 of cystovirus PHI6 as a fusion partner, and process for producing a membrane protein using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0079542 2008-08-13
KR1020080079542A KR101443920B1 (ko) 2008-08-13 2008-08-13 시스토비루스 ｐｈｉ6의 외피막 단백질 Ｐ9을 융합파트너로포함하는 막 단백질 발현벡터 및 이를 이용한 막 단백질의생산방법

Publications (2)

Publication Number Publication Date
WO2010019006A2 true WO2010019006A2 (ko) 2010-02-18
WO2010019006A3 WO2010019006A3 (ko) 2010-06-24

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PCT/KR2009/004525 WO2010019006A2 (ko) 2008-08-13 2009-08-13 시스토비루스 phi6의 외피막 단백질 P9을 융합파트너로 포함하는 발현벡터 및 이를 이용한 막 단백질의 생산방법

Country Status (3)

Country Link
US (1) US8628939B2 (ko)
KR (1) KR101443920B1 (ko)
WO (1) WO2010019006A2 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012064162A3 (ko) * 2010-11-12 2012-09-13 숭실대학교 산학협력단 시스토바이러스 phi12의 외피막 단백질 p9을 융합파트너로 포함하는 막 단백질 발현벡터 및 이를 이용한 막 단백질 제조 방법

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101912631B1 (ko) * 2015-12-03 2018-10-30 국민대학교산학협력단 막 단백질 제조용 융합 단백질, 발현 벡터, 및 이를 이용한 막 단백질 제조방법
KR102137660B1 (ko) 2018-07-23 2020-07-24 강원대학교산학협력단 Cd1 단백질의 생산 및 정제 방법

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006069331A2 (en) * 2004-12-22 2006-06-29 The Salk Institute For Biological Studies Compositions and methods for producing recombinant proteins

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006069331A2 (en) * 2004-12-22 2006-06-29 The Salk Institute For Biological Studies Compositions and methods for producing recombinant proteins

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DREW, D. ET AL.: 'Assembly and overexpression of membrane proteins in Escherichia coli' BIOCHIMICA ET BIOPHYSICA ACTA vol. 1610, 2003, pages 3 - 10 *
NELLI J STROMSTEN ET AL.: 'In vitro DNA Packaging of PRD1: A Common Mechanism for Internal-membrane Viruses' J MOL BIOL. vol. 348, 2005, pages 617 - 629 *
NELLI KARHU (NEE STROMSTEN): 'The genome packaging machinery of dsDNA bacteriophage PRD1' THESIS DISSERTATION, INSTITUTE OF BIOTECHNOLOGY AND DEPARTMENT OF BIOLOGICAL AND ENVIRONMENTAL SCIENCES DIVISION OF GENETICS AND VIIKKI GRADUATE SCHOOL IN BIOSCIENCES UNIVERSITY OF HELSINKI 2006, *
ROOSILD, T.P. ET AL.: 'Characterization of the family of Mistic homologues' BMC STRUCTURAL BIOLOGY vol. 6, no. 10, May 2006, pages 1 - 6 *
ROOSILD, T.P. ET AL.: 'NMR structure of Mistic, a membrane-integrating protein for membrane protein expression' SCIENCE vol. 307, 2005, pages 1317 - 1321 *
ZUO, X. ET AL.: 'Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli' J STRUCT FUNCT GENOMICS vol. 6, 2005, pages 103 - 111 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012064162A3 (ko) * 2010-11-12 2012-09-13 숭실대학교 산학협력단 시스토바이러스 phi12의 외피막 단백질 p9을 융합파트너로 포함하는 막 단백질 발현벡터 및 이를 이용한 막 단백질 제조 방법

Also Published As

Publication number Publication date
KR101443920B1 (ko) 2014-10-01
WO2010019006A3 (ko) 2010-06-24
US8628939B2 (en) 2014-01-14
US20110287484A1 (en) 2011-11-24
KR20100020784A (ko) 2010-02-23

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WO2010019006A2 (ko) 시스토비루스 phi6의 외피막 단백질 P9을 융합파트너로 포함하는 발현벡터 및 이를 이용한 막 단백질의 생산방법

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