WO2009125989A3 - 새로운 질량 분석 신호 증폭 기술 - Google Patents

새로운 질량 분석 신호 증폭 기술 Download PDF

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Publication number
WO2009125989A3
WO2009125989A3 PCT/KR2009/001839 KR2009001839W WO2009125989A3 WO 2009125989 A3 WO2009125989 A3 WO 2009125989A3 KR 2009001839 W KR2009001839 W KR 2009001839W WO 2009125989 A3 WO2009125989 A3 WO 2009125989A3
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WO
WIPO (PCT)
Prior art keywords
target molecules
mass analysis
gold particles
signals
signal amplification
Prior art date
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PCT/KR2009/001839
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English (en)
French (fr)
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WO2009125989A2 (ko
Inventor
박형순
소상완
여운석
이수재
이정록
이주희
김광표
Original Assignee
주식회사 프로바이온
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020080111446A external-priority patent/KR101137420B1/ko
Application filed by 주식회사 프로바이온 filed Critical 주식회사 프로바이온
Priority to JP2011503911A priority Critical patent/JP5369172B2/ja
Priority to US12/937,139 priority patent/US8673653B2/en
Priority to CN200980112571.1A priority patent/CN101990638B/zh
Priority to EP09729499.5A priority patent/EP2278333B1/en
Publication of WO2009125989A2 publication Critical patent/WO2009125989A2/ko
Publication of WO2009125989A3 publication Critical patent/WO2009125989A3/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Inorganic Chemistry (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)

Abstract

본 발명은 새로운 방식의 질량 분석 신호 증폭 기술에 관한 것이다. 더 구체적으로 본 발명에서는 i) 표적 분자와 선택적으로 결합하도록 표면을 개질한 금 입자에 표적 분자의 존부를 알고자 하는 시료를 접촉시킨 다음, ii) 상기 금 입자와 표적 분자 사이에 결합 등 상호 작용이 일어나면, 상기 금 입자에 수식된 저분자 화합물이 질량 분석 신호를 발생하되, iii) 미량으로 존재하는 표적 분자라도 상기 저분자 화합물의 질량 신호를 대규모로 발생하게 함으로써 신호의 증폭이 일어나는 새로운 방식의 검출 방법과 이를 위한 분석 시스템, 그리고 증폭용 금 입자를 제공한다. 본 발명에 따르면, 시료의 전처리 없이 원하는 물질의 신호를 특이적으로 증폭할 수 있으므로 표적 분자를 간편하고 정밀하게 측정할 수 있는 장점이 있다.
PCT/KR2009/001839 2008-04-10 2009-04-10 새로운 질량 분석 신호 증폭 기술 WO2009125989A2 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2011503911A JP5369172B2 (ja) 2008-04-10 2009-04-10 新たな質量分析信号の増幅技術
US12/937,139 US8673653B2 (en) 2008-04-10 2009-04-10 Signal amplification technique for mass analysis
CN200980112571.1A CN101990638B (zh) 2008-04-10 2009-04-10 新的质量分析信号的放大技术
EP09729499.5A EP2278333B1 (en) 2008-04-10 2009-04-10 New signal amplification technique for mass analysis

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2008-0033262 2008-04-10
KR20080033262 2008-04-10
KR1020080111446A KR101137420B1 (ko) 2008-04-10 2008-11-11 새로운 질량 분석 신호 증폭 기술
KR10-2008-0111446 2008-11-11

Publications (2)

Publication Number Publication Date
WO2009125989A2 WO2009125989A2 (ko) 2009-10-15
WO2009125989A3 true WO2009125989A3 (ko) 2010-01-14

Family

ID=41162402

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/001839 WO2009125989A2 (ko) 2008-04-10 2009-04-10 새로운 질량 분석 신호 증폭 기술

Country Status (5)

Country Link
US (1) US8673653B2 (ko)
EP (1) EP2278333B1 (ko)
JP (1) JP5369172B2 (ko)
CN (1) CN101990638B (ko)
WO (1) WO2009125989A2 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2015159534A1 (ja) * 2014-04-16 2017-04-13 富士フイルム株式会社 検出液及びそれを用いたバイオセンシング方法
EP3762029A4 (en) * 2018-03-09 2022-03-23 Ohio State Innovation Foundation PAPER-BASED COLLECTION AND TEST DEVICES FOR BIOLOGICAL SAMPLES
CN111239402B (zh) * 2018-11-29 2021-07-09 北京大学 一种可用于疾病标志物检测的质谱免疫分析方法及应用
CN111735869A (zh) * 2020-05-29 2020-10-02 中山大学 一种蛋白质的检测试剂及检测方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197515B1 (en) * 1994-09-26 2001-03-06 Harvard University Molecular recognition at surfaces derivatized with self-assembled monolayers
KR100737689B1 (ko) * 2006-08-23 2007-07-09 성균관대학교산학협력단 표면 플라즈몬 공명 센서의 신호 증폭 방법

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WO1999029897A1 (de) * 1997-12-05 1999-06-17 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur identifikation von nucleinsäuren durch elektrospray massenspektrometrie
AU1241000A (en) * 1998-10-27 2000-05-15 Clinical Micro Sensors, Inc. Detection of target analytes using particles and electrodes
US20050148101A1 (en) * 1999-01-23 2005-07-07 Bamdad Cynthia C. Interaction of colloid-immobilized species with species on non-colloidal structures
US7413770B2 (en) * 2002-08-01 2008-08-19 E.I. Du Pont De Nemours And Company Ethylene glycol monolayer protected nanoparticles
CN1553188A (zh) * 2003-06-06 2004-12-08 克 宋 微阵列信号放大方法
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Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197515B1 (en) * 1994-09-26 2001-03-06 Harvard University Molecular recognition at surfaces derivatized with self-assembled monolayers
KR100737689B1 (ko) * 2006-08-23 2007-07-09 성균관대학교산학협력단 표면 플라즈몬 공명 센서의 신호 증폭 방법

Non-Patent Citations (4)

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J. R. LEE ET AL.: "Mass spectrometry signal amplification method for attomolar detection of antigens using small-molecule-tagged gold microparticles.", ANGEWANDTE CHEMIE, vol. 120, no. 49, 29 October 2008 (2008-10-29), pages 9660 - 9663, XP008143291 *
S. HOU ET AL.: "Inhibition of Escherichia coli biofilm formation by self-assembled monolayers of functional alkanethiols on gold.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY., vol. 73, no. 13, 4 May 2007 (2007-05-04), pages 4300 - 4307, XP008143299 *
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Also Published As

Publication number Publication date
US8673653B2 (en) 2014-03-18
WO2009125989A2 (ko) 2009-10-15
EP2278333B1 (en) 2013-06-12
JP5369172B2 (ja) 2013-12-18
US20110053292A1 (en) 2011-03-03
EP2278333A2 (en) 2011-01-26
JP2011516880A (ja) 2011-05-26
EP2278333A4 (en) 2011-05-11
CN101990638A (zh) 2011-03-23
CN101990638B (zh) 2014-02-05

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