WO2009123756A1 - Multi-well system - Google Patents

Multi-well system Download PDF

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Publication number
WO2009123756A1
WO2009123756A1 PCT/US2009/002106 US2009002106W WO2009123756A1 WO 2009123756 A1 WO2009123756 A1 WO 2009123756A1 US 2009002106 W US2009002106 W US 2009002106W WO 2009123756 A1 WO2009123756 A1 WO 2009123756A1
Authority
WO
WIPO (PCT)
Prior art keywords
wells
well
antibodies
antibody
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/002106
Other languages
English (en)
French (fr)
Inventor
Chun-Nan Chen
James O. Bowlby
Richard Aleck Jorgensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Single Cell Technology Inc
Original Assignee
Single Cell Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Single Cell Technology Inc filed Critical Single Cell Technology Inc
Publication of WO2009123756A1 publication Critical patent/WO2009123756A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B20/00—Methods specially adapted for identifying library members
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Definitions

  • FIG. 5 A second embodiment of the tray is shown in FIG. 5.
  • the tray is comprised of a frame 8 which includes a plurality of wells 9.
  • the tray of FIG. 5 includes upwardly extending notches 10, 1 1, 12 and 13 in each of the corners.
  • the notches 10-13 fit within the cut-out portions 14, 15, 16 and 17 of the top shown in FIG. 4. In this manner the top and the tray can be precisely aligned with each other.
  • FIGS. 4, 5 and 6 can provide for some alignment. This is generally insufficient for devices which include micro-wells present in very high density and very large numbers.
  • the system such as shown within FIG. 7 and described in Example 2 can be used in such situations.
  • One embodiment of the method of the invention comprises a method of obtaining information from a plurality of isolated cells.
  • the information may be simultaneously obtained from a large number of cells by placing the large number of cells in individual wells. An attempt is made to include a single cell within a single well. However, when carrying out the method involving hundreds, thousands or even tens of thousands of cells and wells it may be that some wells do not include a cell and other wells include more than one cell.
  • mouse IgGl, IgG2a, and IgG2b isotype heavy chain conserved sequence and the K light chain sequence are used for capturing the mouse antibody mRNA in the example illustrated, this represents an embodiment rather than a limitation of the invention. It is entirely within the scope of the present invention to use spotted microarrays wherein a mixture of oligonucleotide probes comprising appropriate sequences can be attached and used to capture all isotypes.
  • a small computer interfaced microscope (not shown) is positioned beneath the aluminum table 503 to image cells in wells using flat field light entering the top of apparatus 500.
  • the column code starts with high bit 606 and ends with low bit 605 : 000011 , or column 3.
  • the row code starts with high bit 608 and ends with low bit 607: 000100, or row 4.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
PCT/US2009/002106 2008-04-05 2009-04-03 Multi-well system Ceased WO2009123756A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US7298508P 2008-04-05 2008-04-05
US61/072,985 2008-04-05
US14157908P 2008-12-30 2008-12-30
US61/141,579 2008-12-30

Publications (1)

Publication Number Publication Date
WO2009123756A1 true WO2009123756A1 (en) 2009-10-08

Family

ID=41135895

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/US2009/002106 Ceased WO2009123756A1 (en) 2008-04-05 2009-04-03 Multi-well system
PCT/US2009/002122 Ceased WO2009123762A2 (en) 2008-04-05 2009-04-03 Method of screening single cells for the production of biologically active agents
PCT/US2009/002086 Ceased WO2009123748A1 (en) 2008-04-05 2009-04-03 Method of obtaining antibodies of interest and nucleotides encoding same

Family Applications After (2)

Application Number Title Priority Date Filing Date
PCT/US2009/002122 Ceased WO2009123762A2 (en) 2008-04-05 2009-04-03 Method of screening single cells for the production of biologically active agents
PCT/US2009/002086 Ceased WO2009123748A1 (en) 2008-04-05 2009-04-03 Method of obtaining antibodies of interest and nucleotides encoding same

Country Status (9)

Country Link
US (4) US9328172B2 (https=)
EP (1) EP2260112A4 (https=)
JP (1) JP5749156B2 (https=)
KR (1) KR101679869B1 (https=)
CN (1) CN102046809A (https=)
AU (1) AU2009232353B2 (https=)
BR (1) BRPI0906544A2 (https=)
CA (1) CA2720472C (https=)
WO (3) WO2009123756A1 (https=)

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Also Published As

Publication number Publication date
CA2720472C (en) 2018-01-02
EP2260112A4 (en) 2013-10-09
US20100035763A1 (en) 2010-02-11
KR20100131517A (ko) 2010-12-15
US9873871B2 (en) 2018-01-23
US8309317B2 (en) 2012-11-13
AU2009232353A1 (en) 2009-10-08
CA2720472A1 (en) 2009-10-08
WO2009123762A2 (en) 2009-10-08
WO2009123748A1 (en) 2009-10-08
US8309035B2 (en) 2012-11-13
US20110190148A1 (en) 2011-08-04
KR101679869B1 (ko) 2016-11-25
US20160201054A1 (en) 2016-07-14
JP5749156B2 (ja) 2015-07-15
BRPI0906544A2 (pt) 2019-01-02
WO2009123762A3 (en) 2009-12-30
JP2011516064A (ja) 2011-05-26
US20090280992A1 (en) 2009-11-12
US9328172B2 (en) 2016-05-03
EP2260112A2 (en) 2010-12-15
AU2009232353B2 (en) 2015-04-30
CN102046809A (zh) 2011-05-04

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