WO2009115852A2 - Unité d'échantillonnage - Google Patents

Unité d'échantillonnage Download PDF

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Publication number
WO2009115852A2
WO2009115852A2 PCT/HU2009/000027 HU2009000027W WO2009115852A2 WO 2009115852 A2 WO2009115852 A2 WO 2009115852A2 HU 2009000027 W HU2009000027 W HU 2009000027W WO 2009115852 A2 WO2009115852 A2 WO 2009115852A2
Authority
WO
WIPO (PCT)
Prior art keywords
ejector
chuck
sampling
unit according
sampling unit
Prior art date
Application number
PCT/HU2009/000027
Other languages
English (en)
Other versions
WO2009115852A3 (fr
Inventor
Tibor Virág
Attila LÁSZLÓ
Béla MOLNÁR
Vivien Angeli
Tibor KRENÁCS
Original Assignee
3Dhistech Kft.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 3Dhistech Kft. filed Critical 3Dhistech Kft.
Publication of WO2009115852A2 publication Critical patent/WO2009115852A2/fr
Publication of WO2009115852A3 publication Critical patent/WO2009115852A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873—Cutting or cleaving
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873—Cutting or cleaving
    • G01N2001/288—Filter punches
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples
    • G01N2001/368—Mounting multiple samples in one block, e.g. TMA [Tissue Microarrays]

Definitions

  • Object of the present invention is a sampling unit for collecting cores of tissue samples from donor blocks and inserting them into recipient blocks, to be used in devices for preparing tissue micro array (henceforth: TMA) blocks, the unit containing carriage travelling on a horizontal rail, a slide moved in vertical direction on said carriage and means for boring tissue blocks, punching tissue samples and expelling tissue samples.
  • TMA tissue micro array
  • the TMA technology can be used for exploration of parameters or other characteristics of diagnostic markers and tumor-cells.
  • TMA devices have been developed for quick and effective microscopic examination of a great number of tissue specimens.
  • US 6,103,518 discloses a device having punch platform for holding a recipient block and a donor block, respectively.
  • a carriage is displaceable between at least first and second positions with respect to said punch platform, said positions precisely defined by detentes.
  • At least first and second punch units are tiltably mounted on said punch platform, each punch unit comprising a punch and a cooperating stylet, wherein one punch unit can make a bore in the recipient block and the other punch unit removes a core from the donor block and in- serts it into the bore of the recipient block.
  • US 6,383,801 discloses another device provided with a base and two dif- ferent punches, one for obtaining the cores of interest and the other for creating the receiving holes in the recipient block. There are two separate axes, one for each punch. Each punch has its own stylet and the axis of each punch is parallel to the axis of its drive. The punches can move only in vertical direction and orientation of the blocks is provided by sensors arranged on the base. The device is more complicated, than the previous one.
  • the core sampling device disclosed in US 2006/0121596 A1 comprises a single punch unit incorporating a core sampling punch and a coaxial core ejector.
  • the punch unit is movably arranged with respect to a table, and the system is provided with an optical system and connected to a computer.
  • the invention provides a sampling unit for collecting and expelling tissue samples, to be used in devices for preparing tissue micro array blocks, the unit containing carriage travelling on a horizontal rail, a slide moved in vertical direction on said carriage and means for boring tissue blocks, punch- ing tissue samples and expelling tissue samples, wherein the carriage is provided with three drives.
  • the first drive moves the slide in vertical direction;
  • the second drive is an electric motor which is detachably coupled to a chuck designated to clamp a drill or a sampling punch provided with an ejector;
  • the third drive moves the ejector in vertical direction within the chuck, which is preferably detachably mounted to the slide.
  • the drill as well as the sampling punch is provided with a connecting sleeve which can be clamped in and rotated with the chuck, meanwhile the ejector is inserted in the sampling punch and is able to move in translation along the same axis, and it is provided at the upper end with a connecting sleeve which can be connected to the third drive for moving the ejector in vertical direction.
  • the chuck receives during operation a drill or a block consisting of a sampling punch and an ejector, and is arranged adjacent to and parallel with the electric motor, and there is a geared transmission between them.
  • the third drive for moving the ejector clamped in the chuck in vertical direction is arranged above the chuck and is connected to a cursor coupled to the third drive by means of a spindle and being moved parallel with the axis of the ejector.
  • the unit is preferably provided with drills and blocks each consisting of a sampling punch and an ejector of different measures. There may be a camera and a lamp in the unit and it may be connected to a computer.
  • the recipient and donor blocks are generally provided with identification codes, and therefor the sampling punch according to the invention is provided a lamp for illuminating said codes and with a camera for identification the blocks.
  • the drives are preferably connected to a computer provided with digital samples corresponding to the samples in the donor block.
  • Digital samples are sets of data stored in the computer and displayed on a screen, containing all characteristics and digitalized parameters of cells or tissues on a glass slide.
  • cylindrical cores will be obtained from a donor block by the sampling punch and the cores will be inserted into the bores of a recipient block in the following steps: a) a donor block and a recipient block is fixed on a table or base, b) identification code of the recipient block is scanned and stored, c) identification code of the donor block is scanned and the corresponding digital sample is searched. d) the images of the donor block and the digital sample are matched and the position of the core to be punched is allocated and e) the core is punched from the donor block and is inserted into the recipient block.
  • the above steps are carried out continuously, preferably in a way that during step e) the next c) and d) steps are performed.
  • the donor block will than be removed and replaced by a new one.
  • the device provided with the unit according to the invention may be simultaneously installed 1 to 4 donor blocks.
  • the main advantage of the unit according to the invention is the excellent flexibility.
  • punches sampling punch, ejector
  • the speed and precision of producing the samples can be further enhanced if the unit is connected to a computer. In this way, there is no need of sophisticated detecting the -A- position of the bores in the recipient block, as these are already arranged in predetermined pattern stored in the system.
  • Fig. 1 shows a schematic perspective view of a preferred embodiment of a device for preparing tissue micro array blocks, provided with the sampling unit according to the invention
  • Fig. 2 shows a sectional view of the chuck of the unit with a sampling punch and ejector
  • Fig. 3 shows a sectional view of the chuck of the unit with a drill
  • Fig. 4 is a schematic perspective view of the unit according to the invention, showing the drives of the punches
  • Fig. 5 is an enlarged section of Fig.4 showing the electric motor connected to the chuck with a drill.
  • Carriage 9 is provided with a slide 10 moving up and down on the carriage.
  • the slide is provided with an electric motor 11 for rotating a chuck 13 mounted on the same slide 10, and connected to the electric motor 11 by geared transmission 12.
  • a cursor 14 Above the chuck 13, there is a cursor 14, which can travel up and down on the slide 10, to move an ejector 15 (Fig. 2), which will be described later.
  • Carriage 9 is further provided with a camera 28 and a lamp 29. Fig.
  • Sampling punch 16 is a tube having a sharp edge at the lower end, intended to facilitate their penetration into the blocks.
  • the tube is assembled with a connecting sleeve 17 to be received in chuck 13 and fixed by screw 18.
  • Ejector 15 sliding within the sampling punch 16 is a rod having an external diameter similar to that of the sample to be inserted into the recipient block, as also usual.
  • the rod is assembled with a connecting sleeve 19 to be received in a seat 20 of the cursor14 and fixed by screw 21 (Fig. 5).
  • Fig. 3 shows the sectional view of a chuck 13 with a drill 22 clamped therein, the drill is a spiral bit, the upper end of which is assembled with a connecting sleeve 23 to be received in chuck 13 and fixed by screw 18.
  • Fig. 4 shows the chuck 13 with the drill 22 and the cursor 14 with the seat
  • Step drive 24 moves the slide 10 together with chuck 13 mounted thereon, by spindle 26, and step drive 25 moves the cursor 14 together with ejector 15 clamped therein, by spindle 17.
  • Fig. 5 is an enlarged part of Fig.4 showing the electric motor 11 connected by gear transmission 12 to the chuck 13 with a drill 22 clamped therein.
  • the device for preparing tissue micro array blocks provided with the sampling unit according to the invention as shown in Fig. 1 is used in the following manner. After the device has been switched on, table 2 moves forward on rail 2, and the operator inserts a recipient block 4 into a seat 6. The block is than fixed in the seat by clip 5 and the code (bar code, matrix code etc.) of the given recipient block 4 is detected by the camera 28 and stored in the computer.
  • a donor block 4 is inserted and fixed in another seat 6.
  • the code of this block is also detected by the camera 28 and stored in the computer, and the operator is looking up the corresponding digital slide, preferably from the data bank of the system.
  • the unit according to the invention provides than automatic sampling from the donor block by lowering and rotating the sampling punch 16 at the parame- tered position and transporting the core to the corresponding bore in the recipient block, wherein the ejector 15 pushes it out from the sampling punch 16 into the bore.
  • Another donor block may be clamped in the table and the position of the next sample to be transferred can be determined. These steps can be repeated until the recipient block (generally having up to 400 - 500 bores to receive samples) is ready. During the process, a list is established on the codes of the blocks and on the pattern of the bores in the recipient block.
  • the empty donor blocks are continuously removed from the device and replaced by new ones.
  • one or two recipient blocks and three or four donor blocks may be held simultaneously.
  • a drill bit 22 should be clamped in the chuck 13 of the unit according to the invention, instead of the punches.
  • the outer diameter of the drill bit should be the same as the inner diameter of the sampling punch.
  • the camera prepares photos before sampling. Different patterns of bores can be prepared, according to the wish of the user. The bores belonging to the chosen pattern will than be prepared on basis of the corresponding computer program.
  • the main advantage of the present invention is that core sizes of wide range can be prepared and the punches as well as the drill bits can be changed easily and quickly. As a result of applying drill bits and rotating sampling punch, much more cores can be arranged in a unit surface of the recipient block as usual.

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne une unité d'échantillonnage destinée à recueillir et à expulser des échantillons tissulaires. Cette unité d'échantillonnage est conçue pour être utilisée dans des dispositifs destinés à préparer des blocs de microréseaux tissulaires (TMA). Cette unité contient un chariot se déplaçant sur un rail horizontal, une lame porte-objet se déplaçant dans la direction verticale sur ledit chariot, et un moyen conçu pour percer des blocs tissulaires, et pour perforer et expulser des échantillons tissulaires. Le chariot (9) présente trois entraînements: le premier entraînement (24) déplace la lame porte-objet (10) dans la direction verticale; le deuxième entraînement est un moteur électrique (11) qui est relié amovible à un mandrin (13) conçu pour serrer une mèche (22), une perforeuse d'échantillonnage (16) ou un éjecteur (15); et le troisième entraînement (25) déplace l'éjecteur (15) dans la direction verticale, à l'intérieur du mandrin (13). Cette unité d'échantillonnage est de préférence reliée à un ordinateur.
PCT/HU2009/000027 2008-03-20 2009-03-17 Unité d'échantillonnage WO2009115852A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HUP0800181 2008-03-20
HU0800181A HUP0800181A2 (en) 2008-03-20 2008-03-20 Apparatus and method for producing tma blocks

Publications (2)

Publication Number Publication Date
WO2009115852A2 true WO2009115852A2 (fr) 2009-09-24
WO2009115852A3 WO2009115852A3 (fr) 2009-11-12

Family

ID=89988152

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/HU2009/000027 WO2009115852A2 (fr) 2008-03-20 2009-03-17 Unité d'échantillonnage

Country Status (2)

Country Link
HU (1) HUP0800181A2 (fr)
WO (1) WO2009115852A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012001013A1 (fr) * 2010-06-30 2012-01-05 Whatman International Limited Appareils et méthodes de découpe de substrats poreux
CN104391126A (zh) * 2014-11-19 2015-03-04 长春迪瑞医疗科技股份有限公司 一种用于生化分析仪移动待测杯的传输装置
CN108194043A (zh) * 2018-01-30 2018-06-22 贵州理工学院 煤矿顶底板稳定性评价用不同直径岩芯钻取装置

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030017446A1 (en) * 2001-07-20 2003-01-23 Ardais Corporation Instruments and methods for creating a tissue microarray
US6699710B1 (en) * 1998-02-25 2004-03-02 The United States Of America As Represented By The Department Of Health And Human Services Tumor tissue microarrays for rapid molecular profiling
US20060121596A1 (en) * 2004-12-02 2006-06-08 Alphelys Core sampling device intended to assemble tissue arrays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6699710B1 (en) * 1998-02-25 2004-03-02 The United States Of America As Represented By The Department Of Health And Human Services Tumor tissue microarrays for rapid molecular profiling
US20030017446A1 (en) * 2001-07-20 2003-01-23 Ardais Corporation Instruments and methods for creating a tissue microarray
US20060121596A1 (en) * 2004-12-02 2006-06-08 Alphelys Core sampling device intended to assemble tissue arrays

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012001013A1 (fr) * 2010-06-30 2012-01-05 Whatman International Limited Appareils et méthodes de découpe de substrats poreux
GB2493893A (en) * 2010-06-30 2013-02-20 Whatman Internat Ltd Apparatuses and methods for cutting porous substrates
GB2493893B (en) * 2010-06-30 2015-04-15 Whatman Internat Ltd Apparatuses and methods for cutting porous substrates
US9321095B2 (en) 2010-06-30 2016-04-26 General Electric Company Apparatuses and methods for cutting porous substrates
CN104391126A (zh) * 2014-11-19 2015-03-04 长春迪瑞医疗科技股份有限公司 一种用于生化分析仪移动待测杯的传输装置
CN108194043A (zh) * 2018-01-30 2018-06-22 贵州理工学院 煤矿顶底板稳定性评价用不同直径岩芯钻取装置
CN108194043B (zh) * 2018-01-30 2023-11-03 贵州理工学院 煤矿顶底板稳定性评价用不同直径岩芯钻取装置

Also Published As

Publication number Publication date
HU0800181D0 (en) 2008-05-28
WO2009115852A3 (fr) 2009-11-12
HUP0800181A2 (en) 2008-09-29

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