WO2009068680A2 - Plateau d'incubation pour l'incubation de puces à protéines - Google Patents

Plateau d'incubation pour l'incubation de puces à protéines Download PDF

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Publication number
WO2009068680A2
WO2009068680A2 PCT/EP2008/066501 EP2008066501W WO2009068680A2 WO 2009068680 A2 WO2009068680 A2 WO 2009068680A2 EP 2008066501 W EP2008066501 W EP 2008066501W WO 2009068680 A2 WO2009068680 A2 WO 2009068680A2
Authority
WO
WIPO (PCT)
Prior art keywords
recess
incubation
sample carrier
depression
receiving
Prior art date
Application number
PCT/EP2008/066501
Other languages
German (de)
English (en)
Other versions
WO2009068680A3 (fr
Inventor
Christian LÖBKE
Ulrike Korf
Holger Sültmann
Annemarie Proustka
Robert Shearrer
Original Assignee
Dkfz Deutsches Krebsforschungszentrum, Stiftung Des Öffentlichen Rechts
Metecon Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dkfz Deutsches Krebsforschungszentrum, Stiftung Des Öffentlichen Rechts, Metecon Gmbh filed Critical Dkfz Deutsches Krebsforschungszentrum, Stiftung Des Öffentlichen Rechts
Publication of WO2009068680A2 publication Critical patent/WO2009068680A2/fr
Publication of WO2009068680A3 publication Critical patent/WO2009068680A3/fr

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/10—Means to control humidity and/or other gases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50855—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using modular assemblies of strips or of individual wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices

Definitions

  • the invention relates to a device for incubating at least one sample carrier with at least one incubation liquid.
  • the invention further relates to an incubation arrangement comprising at least one device for incubating a sample carrier, a sample carrier and at least one incubation liquid.
  • Such apparatus and incubation arrangements are used in particular in the field of biology or medicine to expose incubation surfaces of sample carriers, for example so-called "South", for a longer period of time, for example, chemical and / or microbiological logical incubation liquids.
  • incubation generally refers to the loading or treatment of sample carriers with any type of incubation liquids under controlled conditions, in particular, but not exclusively, incubation processes in biology, medicine or chemistry can be used to control controlled outdoor conditions
  • protein arrays can be incubated on sample carriers with antibody solutions.
  • incubation devices which are often referred to as hybridization chambers
  • incubation devices in the form of small plastic incubation chambers are known, which are glued to the incubation surface of the south and then filled with the incubation liquid.
  • An advantage of such incubation is that on the one hand very little incubation liquid is needed.
  • it is disadvantageous that gluing of the incubation chambers onto the sample carriers is necessary, which is not easy to accomplish in all cases, which can disturb the incubation process and which subsequently causes problems in removing the incubation chambers. can.
  • the incubation solution is not sufficiently moved on the sample carriers even when using rocker mixers. This leads to strong background signals, which severely affect the evaluation.
  • WO 03/106032 A1 describes a hybridization chamber with a base with a recess in which a micro-array can be inserted.
  • the chamber further includes a lid which can be placed on the base, creating a hermetically sealed space for the micro-array.
  • a cover surface is provided on the cover, which is arranged at a distance of approximately 20 mm from the micro-array and runs parallel to this.
  • WO 03/106032 A1 offers the advantage of small volumes of incubation solutions required, but is overall relatively complex and composed of numerous individual parts. This increases the cost of incubation, especially if several samples are to be incubated simultaneously, for example in the context of larger test series.
  • a device for inhibiting at least one sample carrier with an incubation liquid is to be provided, which is easy to implement, requires the least possible amounts of incubation liquid and which should preferably allow the simultaneous incubation of several sample carriers.
  • the incubation liquid may comprise almost any liquid, such as a solution or dispersion, having chemical and / or biological properties.
  • antibody solutions are considered below, which can be used, for example, for incubating protein arrays.
  • the at least one sample carrier may comprise a largely arbitrarily designed sample carrier. It is particularly preferred if the sample carrier comprises a so-called “slide”, ie a plate with a glass and / or ceramic and / or plastic substrate which has at least one incubation surface, that is to say an area to be treated with the incubation liquid. Naturally, a plurality of such incubation surfaces can also be provided.
  • the device further comprises a receiving unit for receiving the sample carrier.
  • This receiving unit is preferably formed in one piece, but may also be designed in several pieces. In order to simplify the production of such device or receiving units, it is particularly preferred if the receiving unit is produced as an injection-molded component or in some other way as a plastic molded part. However, other embodiments are possible, for example embodiments as a machined plastic part.
  • One idea of the present invention is to design the receiving unit in such a way that it functions in the manner of an incubation tray for the inserted sample carrier.
  • the sample carrier is preferably inserted into the receiving unit with the incubation surface facing downwards.
  • an incubation chamber is accommodated in the receiving unit.
  • This incubation chamber can be filled, for example, before or after placement of the sample carrier, for example laterally by means of a pipette. Adhesion forces the incubation liquid, in particular the antibody solution, to attach to the incubation surface of the sample carrier.
  • the receiving unit has at least one receiving surface.
  • This receiving surface may be flat or even uneven (for example, stepped, subdivided or rounded into a plurality of partial surfaces or planes) and, during operation of the device, preferably points vertically upwards.
  • this receiving surface at least a first recess for inserting the sample carrier is added.
  • This first depression may, for example, be adapted to the lateral dimensions of the sample carrier and may, for example, be rectangular or at least approximately rectangular.
  • the receiving surface further has at least one second recess for receiving the incubation liquid, which has a greater depth than the first recess.
  • the second depression is dimensioned and positioned relative to the first depression such that the first depression and the second depression overlap in at least one incubation region.
  • the second recess is substantially completely received within the first recess so that the incubation area is within the first recess.
  • the incubation region that is to say the overlap region between the first depression and the second depression, may in particular be designed such that the at least one incubation area of the at least one sample carrier is completely covered by this incubation area.
  • the downwardly facing surface of the sample carrier which in particular may completely or partially comprise the incubation surface, and the second depression, in particular the part of the second depression which is arranged below the sample carrier, thus together form the incubation chamber in which the incubation liquid is received can be.
  • the incubation chamber preferably has a depth (that is to say a depth below the sample carrier) of 0.2 to 5 mm, in particular of approximately 0.5 mm.
  • the sample carrier is in the opposite direction, that is to say with the active surface or the incubation surface facing downwards, in the receiving unit. This eliminates sticking an incubation chamber or the like. In addition, contamination of the sample carriers, for example by dust or the like, is largely prevented by this "upside-down principle.”
  • the upsid-down structure also allows a comparatively simple design of the receiving unit, which, as stated above, in particular be formed in one piece that can.
  • the incubation arrangement can furthermore comprise at least one incubation liquid accommodated in the second depression or the incubation chamber.
  • This incubation arrangement can furthermore comprise at least one agitation device which is set up in order to set the recording unit in motion, in particular to shake and / or rock it. In this way, a better mixing of the incubation liquid can be achieved.
  • the agitation device may comprise at least one rocking mixer.
  • the incubation arrangement can comprise at least one tempering device, which is set up to set the incubation liquid to a temperature different from the room temperature and to regulate and / or control it at this temperature.
  • this temperature control device may be configured to adjust the Inkubati- onswashkeit a with respect to the room temperature, elevated temperature, especially at a temperature of not more than 40 0 C.
  • other temperature ranges are of course possible.
  • the device described and the incubation arrangement described can be advantageously developed in various ways. These advantageous developments can be implemented individually or in combination.
  • the first recess and / or the second recess at least approximately have a rectangular shape.
  • the rectangular shapes may also have rounded corners, whereby rounded corners may for example be understood to be simple rounding of the edges or, alternatively or additionally, also expansion extensions which, for example, cause a milling out of the recesses and / or another rounding off.
  • the first recess and / or in particular also the second recess can be wholly or partly designed with a polished surface. In this way, microbial contamination of the incubation chamber can be at least largely prevented, and it can even with a small amount of incubation liquid, a smooth movement thereof in the incubation chamber is maintained.
  • the following dimensions have been found for the first recess: a width in the range between 20 and 30 mm, in particular of about 25.5 mm; a length in the range between 50 mm and 100 mm, in particular of about 76 mm; a depth in the range between 0.2 mm and 5 mm, in particular of about 2 mm.
  • a depth of about 0.5 mm has been found to be optimal. At this depth, capillary forces already play a subordinate role for conventional incubation liquids, so that good mixing can still be achieved.
  • the consumption of incubation liquid at this depth is still comparatively low, so that, for example, an amount of 670 ml of incubation liquid can be sufficient.
  • the following dimensions have proved to be advantageous: a width in the range between 15 mm and 30 mm, in particular of about 21.5 mm; a length in the range between 30 mm and 70 mm, in particular of about 52 mm; a depth in the range between 0.2 mm and 5.5 mm, in particular of about 3 mm. It should be noted that the depth of the second device is not necessarily equal to the depth of the incubation chamber, since this usually still the depth of the first recess must be deducted.
  • the above-described optimal depth of the incubation chamber of about 0.5 mm results, but of course other depths (for example, depths of the incubation chamber) between 0.2 and 3 mm) are possible.
  • the incubation chamber preferably has a volume of between 200 ml and 900 ml, in particular, as described above, in a volume of approximately 670 ml.
  • the receiving unit is preferably designed such that it is set up to hold a plurality of sample carriers.
  • a plurality of first recesses and / or a plurality of second recesses may be provided.
  • first wells of several sample carriers and / or the second wells of several sample carriers can also be completely or partially grouped together.
  • individual first recesses may be provided for each sample carrier, which, however, are incubated with one or more common second recesses.
  • Various configurations are possible.
  • first depression and exactly one second depression are assigned to a sample support, so that a single sample support can be incubated by exactly one incubation chamber.
  • suitable fixation devices for these sample carriers may additionally be provided.
  • the weight of each sample carrier is sufficient to press it into the first recess and hold it there. In this way, for example, without additional fixings and / or seals sealing the incubation chamber, which represents a significant advantage of the proposed upside-down arrangement.
  • the device additionally has a lid which, for example, can prevent contamination of the sample carrier and which can also serve as fixation of the sample carrier.
  • a spaced apart from the sample carriers arrangement of the lid is possible.
  • the receiving surface may, for example, have an upper edge for receiving at least one lid, the lid at least partially covering the second and third depressions.
  • the receiving surface may have an upper edge on which the lid can be placed.
  • the upper edge may have a peripheral edge which projects upwards beyond the receiving surface.
  • the cover may, for example, comprise a simple pane and may for example be made of a plastic, in particular PMMA or Plexiglas, and / or may comprise a glass material.
  • an additional fixing device may be provided.
  • this fixing device may be configured such that the device further comprises a lid frame for fixing the lid on the receiving unit.
  • the lid which may be designed to be transparent, for example, continue to be transparent in the region of the incubation chambers, so that the incubation process can be followed in an optical manner.
  • the receiving unit can be designed such that it has on the receiving surface opposite side, in operation so the bottom, a stand base.
  • This pedestal base may for example comprise one or more feet, for example feet in the form of protrusions, on which the device can be placed.
  • the stator base can also comprise a lower edge, for example an at least partially encircling lower edge.
  • the stand base or the peripheral edge can in particular be set up in such a way in that a plurality of receiving sides are stackable, wherein in each case the stator base or lower edge of a first receiving unit grips, for example at least partially surrounds, a second receiving unit located below the first device. In this way, a secure stacking of the devices, in particular the receiving units, possible.
  • the receiving unit can in particular be wholly or partially made of a plastic, for example polystyrene.
  • a plastic for example polystyrene.
  • various shaping processes can be used, for example, known plastic molding processes such as injection molding, transfer molding or the like.
  • machining processes are possible, such as turning, milling or the like.
  • the first recess comprises at least one expansion recess for facilitating the insertion or removal of the sample carrier.
  • Such extension depressions which are also referred to as “tongues” or “ears”, enable, for example, insertion by means of sample tweezers.
  • the insertion recess can, for example, once again have a rounded or also an angular shape.
  • the above-described particularly preferred dimensions of the first recess are each related to the first recess without these at least one extension recess.
  • the second depression can furthermore comprise at least one filling depression projecting laterally beyond the first depression for filling the incubation liquid into the second depression and / or for improved movement of the incubation liquid.
  • incubation liquids can be introduced into the second wells or the incubation chamber by means of a pipette.
  • the filling recesses also serve to equalize the pressure during a movement of the incubation liquid in the incubation chamber.
  • the filling recess may in particular have a rounded shape, but other embodiments are possible.
  • the advantageous dimensions of the second recess described above are dimensions without consideration of the filling recess.
  • filling recesses may be provided. This serves in particular, as described above, the pressure equalization and the improved movement of the incubation liquid in the incubation chamber.
  • at least two opposite to each other substantially with respect to the first and / or second recess Ein colllveriana be provided.
  • essentially opposite may be understood, for example, a diametrical opposite, an arrangement on opposite sides of the wells or a similar arrangement, which allows for a rocking motion in each case in one direction or the other, a pressure equalization.
  • FIG. 1A shows a schematic embodiment of an incubation arrangement according to the invention with a sample carrier and a device for inhibiting the sample carrier;
  • FIG. 1B shows a plan view of a sample carrier used in the incubation arrangement according to FIG. 1A;
  • Figure 2A is a perspective view of a first embodiment of a receiving unit
  • Figure 2B is a plan view of the receiving unit according to Figure 2 A;
  • FIG. 2C shows a sectional view of the receiving unit according to FIG. 2A along the section line B-B in FIG. 2B;
  • Figure 3 is a perspective view of a second embodiment of a
  • Figure 4A is a sectional view of a lid frame for use in a device according to the invention.
  • FIG. 4B is a perspective view of the cover frame according to FIG. 4A.
  • Figure 5 is a plan view of a lid for use in combination with a lid frame according to Figures 4 A and 4B.
  • FIG. 1A schematically shows a possible exemplary embodiment of a device 110 for incubating a sample carrier 112, which forms an embodiment of an incubation arrangement 114 together with the sample carrier 112.
  • An exemplary embodiment of such a sample carrier 112 is shown in FIG. 1B in a plan view looking onto an incubation surface 116.
  • the sample carrier 112 is a so-called "slide" with an edge length Xi of approximately 25 mm and an edge length X 2 of approximately 75.5 mm
  • the incubation surface 116 is approximately 2 mm apart from the distances X3 and X 4 of about 20 mm from the edges of the south 112 spaced.
  • the device 110 has a receiving unit 118, which comprises an upwardly directed receiving surface 120.
  • a first, rectangular recess 122 is inserted, which is dimensioned such that the sample carrier 112 can be received in this recess 122.
  • a second recess 124 is inserted into the receiving surface 120, which defines an incubation chamber 126 together with the incubation surface 116 of the sample carrier 112 when the sample carrier 112 is inserted. While the first recess has a depth X5 of typically 2 to 5 mm, the second recess has a total depth X 6 of, for example, 2.5 to 6 mm. The depths are always calculated starting from the surface of the receiving surface 120.
  • the incubation chamber 126 thus has a depth X 7 , which typically lies between 0.5 and 1 mm.
  • the first recess 122 has an extension recess 128, which is accommodated on the narrow side and which can be designed, for example, in the form of a cutout.
  • This extension recess 128 makes it easier to insert or remove the sample carrier 112 from the first depression 122 acting as a slide chamber.
  • FIGS. 2A to 2C A further exemplary embodiment of a device 110 according to the invention is shown in FIGS. 2A to 2C.
  • FIG. 2A shows a perspective view with a plan view of the receiving surface 120
  • FIG. 2B shows a plan view of the receiving surface 120
  • FIG. 2C shows a sectional view along the section line BB in FIG. 2B.
  • FIG. 2A shows the arrangement of the first depression 122 and the second depression 124. It can be seen that in this embodiment the second depression 124 is completely accommodated within the first depression 122. In each case, 5 first depressions 122 and second depressions 124 are provided, so that the device 110 is configured overall for holding five sample carriers 112. These five sample carriers can be incubated simultaneously.
  • the first recess 122 has a substantially rectangular shape with a rectangular edge length of approximately 25.4 ⁇ 76 mm 2 and is thus configured, for example, for receiving the sample carrier 112 according to FIG.
  • the corners of this rectangle of the first recess 122 have rounded portions 130, which allow, for example, a simplification of a milling out of the first recess 122 and / or facilitate insertion of the sample carrier 112 or its removal.
  • the first recess 122 has the expansion recess 128 already described.
  • the second recess 124 is substantially rectangular in shape in this embodiment, wherein the rectangle, for example, has an edge length of 21.4 x 52 mm 2 . All measures given in the context of this figure description are to be understood as possible embodiments only. Again, the second recess 124 has rounded edges 130.
  • the second recess 124 in the exemplary embodiment illustrated in FIGS. 2A to 2C furthermore has two filling recesses 132, which in this exemplary embodiment at two diametrically opposite corners of the second recess 124 are arranged.
  • Other arrangements are also possible, for example a different number of filling recesses 132 (for example a filling recess or three, four or more filling recesses 132) and / or another arrangement of these filling recesses, such as the arrangement shown below in FIG.
  • These filling recesses 132 protrude laterally beyond the first depression 122 and, in the present exemplary embodiment, are removed. rounds.
  • filling recesses 132 allow infestation of the incubation chamber 126 with inserted sample carrier 112, for example by means of a pipette.
  • the filling depressions 132 thus act as "filling nozzles" for the incubation chamber 126.
  • the incubation solution thereby collects in the incubation chamber 126 or the second depression 124 and wets the incubation surface 116 of the sample carrier 112 lying above it, for example by adhesive force
  • the sample carrier 112 is designed, for example, such that it is coated with nitrocellulose 134 in the region of the incubation surface 116.
  • the filling recesses 132 allow air to escape from the incubation chamber 126, for example, when filling the incubation solution and / or shaking the device 110.
  • the filling recesses 132 are diametrically opposite each other with respect to the second recess 124 designed.
  • “opposing” means an arrangement at diametrically opposite corners of the rectangle of the second recess 124.
  • Other embodiments of "opposite one another" are also conceivable, as evident for example from FIG.
  • a receiving unit 118 of a device 110 is shown, which essentially corresponds in terms of its structure and function to the receiving unit 118 according to FIGS.
  • filling recesses 132 are again provided per second recess 124, which in turn are likewise arranged opposite one another, but in this case the filling recesses 32 are arranged on opposite longitudinal sides of the rectangle of the second recess 124.
  • the filling recesses 132 of adjacent sample receptacles that is to say adjacent first depressions 122 or second depressions 124, are offset relative to one another so that these filling depressions 132 do not interfere with one another and nevertheless incubate the largest possible number of sample carriers 112 on one and the same device 110 can be.
  • the two exemplary embodiments of the device 110 according to the invention furthermore have an upper edge 136.
  • This upper edge 136 has a support surface 138 on which a lid 140 shown in FIG. 5 can be placed.
  • This cover 140 which for example has dimensions of 97.5 ⁇ 189.5 mm 2 and a thickness of 2 mm and which is preferably provided with rounded corners, is preferably designed to be transparent so that even when the cover is placed on the receiving unit 118 the sample carrier 112 or the incubation process can be observed.
  • a Plexiglas cover can be used for this purpose.
  • the upper edge 136 also has a rectangular shape with rounded corners and outer dimensions of 190 x 98 mm 2 , such as a thickness of 1 mm, for example.
  • the upper edge 136 stands, for example, by 2 mm on the receiving surface 120 via.
  • a cover frame 142 In order to fix the cover 140 on the support surface 138 of the upper edge 136, for example, a cover frame 142, as shown in Figures 4A (sectional view) and 4B (perspective view) can be used.
  • This lid frame 142 has an inner opening 144 and is again of a rectangular shape.
  • the lid frame outer dimensions of 198 x 106 mm 2 may have a height X 7 of 10 mm and a thickness Xs, for example, 2 mm.
  • the corners and also the edges of the cover frame 142 can be made rounded.
  • the sample carriers 112 are thus first inserted with their incubation surface 116 down into the first depressions 122. Subsequently, the incubation chambers 126 are filled with one or more incubation liquids. Then, the lid 140 is placed on the upper edge 136 and fixed by means of the lid frame 142. Subsequently, the device 110 can be placed on an agitation device, for example on a shaker, and tempered, for example, with a suitable temperature control device (also not shown in the figures).
  • the receiving unit may additionally have a stand base 146 on its underside.
  • This pedestal base 146 is designed in this embodiment as a circumferential lower edge 148 and is externally attached to the rest of the receiving unit 118.
  • the peripheral edge 148 is dimensioned such that two receiving units 118 can be stacked as modules one above the other. In this way, a stack of several recording 118 which is secured against slipping by the lower edge 148 and which can be subjected as a stack to an incubation process and / or which allows a stacked storage of receiving units 118.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un dispositif (110) utilisé pour l'incubation d'au moins un porte-échantillons (112) avec au moins un liquide d'incubation. Le porte-échantillons (112) présente au moins une surface d'incubation (116). Le dispositif (110) selon l'invention comprend au moins une unité de réception (118) pourvue d'au moins une surface de réception (120) présentant au moins une première cavité (122) dans laquelle le porte-échantillons (112) peut être placé. La surface de réception (120) présente par ailleurs au moins une deuxième cavité (124) destinée à recevoir le liquide d'incubation, la profondeur de cette deuxième cavité (124) étant supérieure à celle de la première cavité (122). La première cavité (122) et la deuxième cavité (124) se chevauchent de telle sorte que le porte-échantillons (112) et la deuxième cavité (124) forment une chambre d'incubation (126) lorsque le porte-échantillons (112) est placé dans la première cavité (122).
PCT/EP2008/066501 2007-11-30 2008-12-01 Plateau d'incubation pour l'incubation de puces à protéines WO2009068680A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE202007016855.1 2007-11-30
DE200720016855 DE202007016855U1 (de) 2007-11-30 2007-11-30 Inkubationsschale für Protein-Array-Inkubationen

Publications (2)

Publication Number Publication Date
WO2009068680A2 true WO2009068680A2 (fr) 2009-06-04
WO2009068680A3 WO2009068680A3 (fr) 2009-11-05

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WO (1) WO2009068680A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104602818A (zh) * 2012-07-11 2015-05-06 欧蒙医学诊断技术有限公司 用于组织切换或细胞涂片的显微镜检验的方法和分析装置

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020192701A1 (en) * 2001-03-09 2002-12-19 Adey Nils B. Laminated microarray interface device
WO2003018181A1 (fr) * 2001-08-31 2003-03-06 Advalytix Ag Element de mise en mouvement pour petites quantites de liquide
WO2003106032A1 (fr) * 2002-06-14 2003-12-24 Axaron Bioscience Ag Chambre d'hybridation
US20040248287A1 (en) * 2003-03-28 2004-12-09 Qianjin Hu Multi-array systems and methods of use thereof
EP1512462A2 (fr) * 2003-09-03 2005-03-09 Eppendorf Ag Couvercle de chambre d'hybridation
WO2005024385A2 (fr) * 2003-09-09 2005-03-17 Biogenex Laboratories Systeme de traitement d'echantillons

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020192701A1 (en) * 2001-03-09 2002-12-19 Adey Nils B. Laminated microarray interface device
WO2003018181A1 (fr) * 2001-08-31 2003-03-06 Advalytix Ag Element de mise en mouvement pour petites quantites de liquide
WO2003106032A1 (fr) * 2002-06-14 2003-12-24 Axaron Bioscience Ag Chambre d'hybridation
US20040248287A1 (en) * 2003-03-28 2004-12-09 Qianjin Hu Multi-array systems and methods of use thereof
EP1512462A2 (fr) * 2003-09-03 2005-03-09 Eppendorf Ag Couvercle de chambre d'hybridation
WO2005024385A2 (fr) * 2003-09-09 2005-03-17 Biogenex Laboratories Systeme de traitement d'echantillons

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104602818A (zh) * 2012-07-11 2015-05-06 欧蒙医学诊断技术有限公司 用于组织切换或细胞涂片的显微镜检验的方法和分析装置

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WO2009068680A3 (fr) 2009-11-05

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