EP2604342B1 - Chambre d'analyse dotée d'une plaque de séparation - Google Patents

Chambre d'analyse dotée d'une plaque de séparation Download PDF

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Publication number
EP2604342B1
EP2604342B1 EP11193029.3A EP11193029A EP2604342B1 EP 2604342 B1 EP2604342 B1 EP 2604342B1 EP 11193029 A EP11193029 A EP 11193029A EP 2604342 B1 EP2604342 B1 EP 2604342B1
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EP
European Patent Office
Prior art keywords
reservoir
side wall
sample chamber
chamber according
plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP11193029.3A
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German (de)
English (en)
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EP2604342A1 (fr
Inventor
Elias Horn
Roman Zantl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ibidi GmbH
Original Assignee
Ibidi GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ibidi GmbH filed Critical Ibidi GmbH
Priority to DK11193029.3T priority Critical patent/DK2604342T3/da
Priority to EP11193029.3A priority patent/EP2604342B1/fr
Priority to US13/705,759 priority patent/US9333503B2/en
Priority to CN201210536279.6A priority patent/CN103157524B/zh
Publication of EP2604342A1 publication Critical patent/EP2604342A1/fr
Application granted granted Critical
Publication of EP2604342B1 publication Critical patent/EP2604342B1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls

Definitions

  • the present invention relates to a sample chamber for microscope examinations comprising a reservoir for receiving a sample.
  • sample chambers Particularly in the field of cell microscopy, a wide variety of forms of sample chambers are known. Almost all sample chambers have structures for receiving a sample, for example in the form of microfluidic channels or reservoirs. Examples of such sample chambers are in EP 1 886 792 A2 , of the WO 2008/149914 A2 , of the WO 2005/079985 or the DE 101 48 210 shown.
  • sample chambers Possible fields of use for such sample chambers are in particular in the field of microscopy of molecules or cells.
  • the samples to be examined are placed with a liquid in a reservoir of the sample chamber and can then be examined by high-resolution methods (for example, transmitted light microscopy, fluorescence microscopy, confocal microscopy, etc.).
  • a sample chamber in which a meniscus is to be avoided or minimized by a cover plate.
  • the outer surface of the sample chamber is contaminated when liquid from the inlet through which the liquid can be filled into the reservoir exits. Cross-contamination with samples in adjacent reservoirs can not be ruled out in this case either.
  • a cover used to cover the sample chamber may be contaminated in this case.
  • a meniscus-free microscopy is possible by a liquid to be examined is filled into the lower part of the reservoir, so that the filling level completely wets the lower surface of the partition plate.
  • the liquid can be introduced via the inlet / outlet in the lower part of the reservoir.
  • the partition plate is disposed at a height which is less than the minimum height of the side wall, and the upper part of the reservoir is laterally completely bounded by the side wall. Consequently, it is possible to prevent or at least minimize contamination of the outside of the sample chamber or cross-contamination when excess liquid exits the inlet / outlet. In other words, leaking fluid would first be collected in the upper reservoir without escaping from the reservoir to create contamination of the outer surfaces of the sample chamber. The excess liquid in the upper part reservoir can then optionally be removed by pipetting.
  • the reservoir may in particular be a reservoir for receiving a liquid.
  • the sample may either correspond to a liquid or be suspended in a liquid.
  • cells may be considered as a sample.
  • the height of the side wall and / or the height at which the partition plate is arranged in the reservoir can be measured in particular from the bottom of the reservoir.
  • the height of the side wall and / or the height in the reservoir in which the partition plate is arranged can also be determined from a flat surface on which the sample chamber rests, in particular during operation. The height can be measured in particular up to the upper edge of the side wall.
  • the side wall of the reservoir can in particular limit the reservoir completely laterally so that a liquid filled into the reservoir does not flow laterally out of the reservoir can escape.
  • the side wall may be formed circumferentially, in particular wherein the side wall does not comprise through openings.
  • the side wall may in particular have a constant height.
  • the minimum height of the side wall corresponds to the (constant) height of the side wall.
  • the side wall may also have a variable height.
  • the minimum height of the side wall can thus correspond in particular to that height up to which a liquid can be filled into the reservoir without it leaking out of the reservoir.
  • the height at which the partition plate is arranged may in particular be less than or equal to three quarters, in particular less than or equal to half, in particular less than or equal to a quarter, of the minimum height of the side wall.
  • parallel to the base plate may in particular mean parallel to a planar region of the base plate, in particular wherein the sample chamber rests at least partially on this planar region during operation.
  • Parallel to the bottom plate may also mean parallel to the region of the bottom plate on which the sample chamber rests during operation.
  • parallel to the base plate may mean that the angle between the base plate, in particular the flat region of the base plate, and the separating plate is less than 5 °, in particular less than 1 °.
  • the partition plate may be connected to the side wall, in particular with a side edge or with two opposite sides of the side wall.
  • the partition plate may be connected only to one side edge or to two opposite sides of the side wall.
  • the partition plate may at least partially not be connected to the side wall.
  • the partition plate can be fixed, in particular not non-destructively releasably connected to the side wall.
  • the side wall can be glued or welded to the partition plate.
  • the side wall and the partition plate may also be integrally formed.
  • the partition plate may be loosely connected to the side wall.
  • a higher flexibility with regard to the samples to be examined can be achieved.
  • the sample chamber can be designed such that the partition plate is exchangeable.
  • the upper part reservoir can be open towards the outside, so that the partition plate can be removed and / or inserted via this opening.
  • the side wall may have a, in particular circumferential, edge on which the partition plate rests loosely.
  • a simple and secure loose connection of the partition plate with the side wall is possible.
  • this allows the height at which the partition plate is arranged to be precisely determined.
  • the edge may in particular be formed by a projection or a shoulder.
  • the inside of the side wall could also be arranged at a certain height in the reservoir.
  • this reversible connection can be made somewhat more stable, since a frictional connection between the partition plate and the side wall can be formed by the tapering of the side wall.
  • one or more Einrastelmente can be introduced or arranged on the side wall, so that the partition plate is thereby positively connected to the side wall.
  • the partition plate may have a geometry that corresponds to the geometry of the reservoir. This can provide an optimal surface for microscopy without meniscus. For example, if the reservoir has a rectangular cross section, the partition plate may also be rectangular.
  • the partition plate may be liquid-impermeable, porous or membrane-shaped. If a membrane or other porous material is chosen as the separating plate, the pores can be dimensioned such that from below the liquid is drawn into the separating plate, but due to the surface tension of the water the upper side of the separating plate is not wetted.
  • pore sizes of 0.2 .mu.m to 50 .mu.m, in particular from 0.2 .mu.m to 20 .mu.m are advantageous.
  • the membrane may be formed as a permeable or semipermeable membrane.
  • the partition plate may have a thickness between 0.1 mm and 10 mm, in particular between 1 mm and 3 mm. As a result, sufficient stability of the partition plate can be achieved.
  • the partition plate may in particular be rigid. As a result, it is possible to prevent the partition plate from being deformed under the pressure of a liquid arranged in the reservoir, as a result of which the meniscus could no longer be reliably prevented.
  • the partition plate may have a flexural rigidity greater than or equal to the flexural rigidity of a square, plane PC (polycarbonate) plate having a side length of 1 cm, a constant thickness of 1 mm, and a rectangular cross section.
  • a flexural rigidity greater than or equal to the flexural rigidity of a square, plane PC (polycarbonate) plate having a side length of 1 cm, a constant thickness of 1 mm, and a rectangular cross section.
  • the flexural rigidity of the separator plate may be more than 190,000 N ⁇ mm 2 .
  • the bending stiffness corresponds to the product of the modulus of elasticity of the material of the separating plate and the geometrical moment of inertia of the separating plate.
  • the elastic modulus can be determined by a method according to DIN 53457.
  • the flexural rigidity may be related in particular to a bending by a force perpendicular to the surface of the partition plate, in particular perpendicular to the surface of the partition plate which is arranged parallel to the bottom plate.
  • the at least one inlet / outlet can be formed through an opening in the partition plate or through an opening between the partition plate and the side wall. This allows a simple and direct connection between the upper and the lower part of the reservoir.
  • the opening in the partition plate may be, for example, a through hole.
  • An opening between the partition plate and the side wall may be at least partially formed by a lateral recess in the partition plate.
  • the lateral recess can be provided in particular in the region of a corner of the partition plate.
  • the opening between the partition plate and the side wall may also be formed at least in part by the partition plate being at least partially spaced from the side wall.
  • an elongated passage opening between the partition plate and the side wall can be formed from the upper to the lower part reservoir.
  • the opening between the partition plate and the side wall may extend over the entire length of one of the side walls extend. This allows easier filling of the lower reservoir.
  • the opening in particular in the form of a through hole in the partition plate, may have a diameter of at least 0.6 mm, in particular at least 0.8 mm.
  • the lower part reservoir can advantageously be filled with a pipette, since the dimensions of the opening thus correspond at least to the typical size of a pipette tip.
  • the upper and the lower part of the reservoir can also be connected by two inlets / outlets, which are arranged in particular on opposite sides of the reservoir.
  • two inlets / outlets which are arranged in particular on opposite sides of the reservoir.
  • a good venting can be achieved during filling of the lower part of the reservoir.
  • the Applicant has surprisingly found that this also allows a more homogeneous distribution of suspended in a liquid samples in the lower part of the reservoir can be achieved.
  • the ratio of the area of the separating plate to the base surface of the reservoir can be greater than 0.7, in particular greater than 0.8. As a result, the largest possible area for microscopy examination can be provided.
  • the reservoir may in particular have a rectangular or square base. In principle, however, any other geometries of the reservoir, in particular the base of the reservoir, are possible.
  • the side wall may in particular comprise four side walls.
  • the side walls may enclose with the bottom plate an angle between 80 ° and 90 °, in particular 90 °.
  • the reservoir can be open at the top.
  • the reservoir can be designed such that the upper part of the reservoir is freely accessible from the outside.
  • simple filling methods for introducing the samples into the lower part reservoir can be made possible. For example, this pipetting can be enabled or facilitated.
  • the sample chamber may further comprise a further reservoir for receiving a sample, wherein the further reservoir is bounded by the bottom plate and a side wall, and wherein in the further reservoir a plate parallel to the bottom plate is arranged, wherein the partition plate is disposed in the further reservoir at a height which is less than the minimum height of the side wall, so that the further reservoir is subdivided into an upper and a lower partial reservoir, the lower part reservoir and the upper part reservoir are bounded laterally completely by the side wall, and wherein the upper and the lower part reservoir are connected by at least one inlet / outlet.
  • the sample chamber can have a plurality of reservoirs described above, each with a corresponding partition plate. This allows different investigations to be carried out with a single sample chamber.
  • the sample chamber may have two or more reservoirs, each of the reservoirs comprising a partition plate as described above in the arrangement described above.
  • the sample chamber may comprise exactly two, four, eight, twelve, 24, 48, or 96 reservoirs.
  • the reservoir and the further reservoir may partially have a common side wall.
  • the reservoir and the further reservoir may be two adjacent reservoirs that share at least part of the sidewall.
  • the sample chamber may also include a lid for closing the reservoir, in particular wherein the lid rests at least partially flat against the lateral outer surface of the side wall.
  • a lid for closing the reservoir, in particular wherein the lid rests at least partially flat against the lateral outer surface of the side wall.
  • the lid can seal the liquid reservoir in particular gas-tight or gas-permeable. With such a lid also the risk of contamination of the liquid contained in the reservoir can be reduced. In a gas-tight closure, in particular, investigations under a predetermined gas atmosphere can be carried out.
  • the lid and the side wall may be formed such that the lateral outer surface of the lid is flush with the lateral outer surface of the side wall. This makes it easier to grip this device, which simplifies in particular the manual transport and makes safer.
  • a flat concern of the lid on the lateral outer surface can ensure a secure and tight connection between the lid and side wall.
  • the lid and / or the side wall may have a latching element and / or a recess for receiving the latching element.
  • the lid can be firmly connected to the side wall.
  • the cover can also rest loosely on the side wall, for example on an edge, in particular circumferential, on the outside of the side wall.
  • the edge can be formed by a projection or by a paragraph.
  • the cover may in particular be designed such that it covers all reservoirs of the sample chamber.
  • the side wall and the partition plate can be integrally formed, in particular be formed from an injection molded part. As a result, a simple production of the sample chamber is possible.
  • the bottom plate may in particular be firmly connected to the side wall, in particular liquid-tight.
  • the bottom plate may be connected to the side wall by means of adhesive, solvent, UV treatment, radioactive treatment, laser treatment or thermal welding.
  • the thermal welding can be flat or strip-shaped, in particular only along the edge of the bottom plate and / or the side wall, be carried out. This advantageously allows a firm connection of side wall and bottom plate.
  • the sidewall may also be bonded to the separator plate by means of an adhesive, solvent, UV treatment, radioactive treatment, laser treatment or thermal welding.
  • the bottom plate may be formed in particular planar. Planar can mean in this context that two opposing surfaces of the bottom plate are formed plane-parallel.
  • the bottom plate may also be formed only partially planar.
  • the bottom plate may have a thickness of 1 .mu.m to 300 .mu.m, preferably 100 .mu.m to 200 .mu.m. Such a bottom plate advantageously allows an application of inverse microscopy.
  • the thickness may correspond to the maximum thickness of the bottom plate. In the case of a planar bottom plate, the thickness is constant.
  • the bottom plate may have a depression in the region of the reservoir.
  • the thickness of the bottom plate can be reduced, which has a positive effect on the Use for microscopy examinations may have, while outside the reservoir area is a greater thickness, which may have a positive effect on the stability of the sample chamber.
  • the bottom plate may also be formed as an injection molded part.
  • the sample chamber may in particular comprise a cover plate which is fixedly connected to the bottom plate, wherein a recess is provided in the cover plate, so that a reservoir is formed by the bottom plate and the recess.
  • the side wall may be part of a cover plate connected to the bottom plate.
  • the bottom plate, the cover plate, in particular the side wall, and / or the separating plate may be a plastic, in particular COC (cyclo-olefin copolymer), COP (cyclo-olefin polymer), PE (polyethylene), PS (polystyrene), PC (polycarbonate) and / or PMMA (polymethyl methacrylate).
  • the plastic may have low birefringence (such as glass) and / or intrinsic fluorescence substantially equal to the intrinsic fluorescence of a conventional coverslip. Such a high-quality plastic can improve microscopy studies, especially in the application of fluorescence microscopy.
  • the bottom plate may comprise a flexible material, for example a foil.
  • the bottom plate may also consist of a glass, in particular a cover glass.
  • the base area of the sample chamber in particular the bottom plate, may have the dimensions of a conventional microscope slide, in particular a width of about 25.5 mm and a length of about 75.5 mm, or the dimensions of a multi-well plate, in particular in a width of approx 85.6 mm and a length of approx. 127.6 mm
  • the reservoir may have a volume of between 10 ⁇ l and 10 ml, preferably between 20 ⁇ l and 5 ml.
  • the height of the reservoir may be between 25 ⁇ m and 20 mm, preferably between 0.1 mm and 5 mm.
  • The, in particular maximum, diameter of the reservoir, or the edge length of e.g. rectangular reservoirs may be between 0.5 mm and 50 mm, preferably between 1 mm and 25 mm.
  • this cover plate may include a planar portion that is parallel to the bottom plate.
  • the height of the sample chamber in a planar region of such a cover plate may be between 0.5 mm and 10 mm, preferably between 1 mm and 2 mm, in particular 1.7 mm. If the cover plate of the sample chamber has an elevation in which a recess is formed, then the volume of a reservoir formed in this way can be between 50 ⁇ l and 3 ml, preferably between 80 ⁇ l and 2.5 ml, lie.
  • the height of an elevation starting from a planar region of the cover plate may be between 1 mm and 2 cm, preferably between 5 mm and 1 cm.
  • the inner surface of the reservoir may be at least partially hydrophilized.
  • the penetration of undesired substances, for example hydrophobic solvents, into the material of the sample chamber can be prevented or at least minimized.
  • the inner surface of the sample chamber may be hydrophilized in the lower part reservoir.
  • the inner surface of the reservoir may be at least partially hydrophilized by introducing a plasma or plasma gases or reactive gases such as ozone or nitrogen oxides into the reservoir.
  • the plasma can be passed in particular through the at least one inlet / outlet in the lower part of the reservoir. As a result, effective hydrophilization of a reservoir surface can be achieved.
  • FIG. 1 an exemplary sample chamber for microscope examinations, for example for fluorescence microscopy, is shown.
  • the exemplary sample chamber 1 comprises a bottom plate 2 and a side wall 3. Through the bottom plate 2 and the side wall 3, a reservoir is limited, which is open at the top.
  • a partition plate 4 is arranged so that the reservoir is subdivided into a lower part reservoir 5 and an upper part reservoir 6.
  • the side wall 3 has a constant height in this case.
  • the partition plate 4 is arranged at a height in the reservoir, which is less than the height of the side wall third
  • the reservoir has a side wall 3 with variable height.
  • the partition plate is placed at a height which is less than the minimum height of the side wall.
  • Both the lower part of the reservoir 5 and the upper part of reservoir 6 are laterally completely bounded by the side wall 3.
  • the lower part reservoir 5 and the upper part reservoir 6 are arranged in alignment in this example one above the other.
  • the upper part of the reservoir 6 is connected to the lower part of the reservoir 5 through a first inlet / outlet 7 and a second inlet / outlet 8.
  • a sample in particular suspended in a liquid, can be introduced into the lower part reservoir 5. If the lower part of the reservoir 5 is filled with a liquid so that the filling height is completely wetted the underside of the partition plate 4, no meniscus forms.
  • the height at which the partition plate 4 is arranged may correspond to, in particular, half of the minimum height of the side wall 3 or less.
  • the flexural rigidity of the partition plate 4 may in particular be more than 191666 N ⁇ mm 2 . This corresponds to the bending stiffness of a square separator plate with a thickness of 1 mm and a side length of 1 cm, consisting of polycarbonate.
  • the flexural rigidity is based on a bend perpendicular to the surface of the partition plate 4, which is arranged parallel to the bottom plate 2.
  • FIG. 2 shows a top view of an exemplary sample chamber after FIG. 1 ,
  • the two inlets / outlets 7, 8 are formed in that the partition plate 4 is at least partially spaced from the side wall 3.
  • the partition plate 4 is connected to only two opposite sides of the side wall 3.
  • the partition plate 4 is spaced, whereby a passage opening between the upper part of the reservoir 6 and the lower part of the reservoir 5 is formed.
  • the inlets / outlets 7, 8 thus have the shape of a slot.
  • widenings are provided at the ends of the slot-shaped openings. These can facilitate the filling, for example with a pipette.
  • These widenings are formed by lateral recesses at the corners of the separating plate 4.
  • the distance between the partition plate 4 and the side wall 3 is in the region of the widenings more than 0.6 mm, in particular more than 0.8 mm.
  • the partition plate 4 is fixedly connected to the side wall 3, for example by thermal welding.
  • the partition plate 4 could also be connected loosely to the side wall 3.
  • the side wall 3 could have at least on two opposite sides an edge on which the partition plate 4 can rest loosely.
  • FIG. 3 shows a further cross-sectional view of an exemplary sample chamber.
  • this sample chamber has a bottom plate 2, a side wall 3 and a partition plate 4.
  • a reservoir for receiving a sample is limited.
  • the partition plate 4 the reservoir is divided into a lower part of the reservoir 5 and an upper part of the reservoir 6, which are connected by two inlets / outlets 7, 8.
  • the reservoir is closed by a cover 9 in this example.
  • the cover 9 can close the reservoir gas-tight or gas-permeable. Such a lid also reduces the risk of contamination of the sample contained in the reservoir.
  • investigations can be carried out under a predetermined gas atmosphere.
  • the side wall 3 on its outer surface on an edge, which is formed by a paragraph and on which the lid 9 rests.
  • the outer surface of the lid 9 is aligned with the outer surfaces of the side wall 3. This allows the sample chamber better grip, which makes hand transport easier and safer.
  • the cover 9 is in this example also flat against the lateral outer surface of the side wall 3. This ensures a secure and tight connection between the cover 9 and side wall 3.
  • FIG. 4 shows a perspective view of another exemplary sample chamber 1.
  • this sample chamber 1 two adjacent reservoirs are provided, each bounded by a side wall 3 and a bottom plate 2.
  • a parallel to the bottom plate 2 partition plate 4 is arranged, which is liquid impermeable and the respective reservoir into a lower part of the reservoir 5 and an upper part of reservoir 6, which are connected by inlets / outlets 7, 8 shares.
  • the partition plate 4 could alternatively be porous or formed as a membrane.
  • an edge 10 can be seen, on which a lid for closing the reservoirs can rest.
  • FIG. 5 shows an exemplary sample chamber after FIG. 4 with a lid 9 for closing the reservoirs.
  • FIG. 6 is the exemplary sample chamber of FIG. 5 shown, both reservoirs are closed by the lid 9.
  • the inner surface of the reservoirs shown above may also be at least partially hydrophilized, so have a hydrophilic layer.
  • a hydrophilic layer can prevent or at least minimize the migration of undesired substances, for example solvents, into the material of the sample chamber.
  • the layer may have a surface tension of more than 70 mN / m, in particular more than 72 mN / m. In this way, preferably hydrophilic properties are achieved.
  • a hydrophilic layer may be, for example, an SiO x layer. Such a hydrophilic layer can be achieved, for example, by plasma technologies in which SiO x is deposited. SiO x has a surface tension of over 72 mN / m.
  • the surface tension (or surface energy) is determined according to ISO 8296: 2003 (Plastics - Films and webs - Determination of wetting tension, ISO 8296: 2003).
  • ISO 8296 (formerly DIN 53 364) regulates the assessment of the average wettability of plastics.
  • the criterion is the behavior of the edge of brushstrokes with test inks. Test inks with different surface tensions are used. If the edge of the brush stroke contracts within 2 seconds, the measurement is repeated with the next lower value. If the edge of the brush stroke runs outwards, the measurement is repeated with the next highest value.
  • the (critical) surface energy is the value of the test liquid whose edge stops just 2 seconds.
  • the geometry of the reservoirs is not limited to the square shape shown in the figures. Any other geometries are possible.
  • the reservoirs can also be cylindrical.

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  • Analytical Chemistry (AREA)
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Claims (16)

  1. Chambre d'échantillon (1) pour analyses au microscope, comprenant
    un réservoir destiné à accueillir un échantillon, le réservoir étant délimité par une plaque de fond (2) et une paroi latérale (3), et
    une plaque séparatrice (4) agencée dans le réservoir et parallèle à la plaque de fond (2),
    chambre d'échantillon
    dans laquelle la plaque séparatrice (4) est agencée dans le réservoir, à une hauteur qui est inférieure à la hauteur minimale de la paroi latérale (3), de sorte qu'elle subdivise le réservoir en un réservoir partiel supérieur et inférieur (6, 5),
    dans laquelle le réservoir partiel inférieur (5) et le réservoir partiel supérieur (6) sont délimités latéralement, en totalité, par la paroi latérale (3), et dans laquelle le réservoir partiel supérieur et celui inférieur (6, 5) sont reliés par au moins une arrivée/sortie d'écoulement (7, 8).
  2. Chambre d'échantillon selon la revendication 1, dans laquelle la paroi latérale (3) présente une hauteur constante.
  3. Chambre d'échantillon selon la revendication 1 ou la revendication 2, dans laquelle la plaque séparatrice (4) est reliée à la paroi latérale (3), notamment à une bordure latérale ou à deux côtés opposés de la paroi latérale (3).
  4. Chambre d'échantillon selon la revendication 3, dans laquelle la plaque séparatrice (4) est reliée de manière amovible à la paroi latérale (3).
  5. Chambre d'échantillon selon l'une des revendications précédentes, dans laquelle la paroi latérale (3) présente une bordure (10), notamment périphérique, sur laquelle repose de manière amovible la plaque séparatrice (4).
  6. Chambre d'échantillon selon l'une des revendications précédentes, dans laquelle ladite au moins une arrivée/sortie d'écoulement (7, 8) est formée par une ouverture dans la plaque séparatrice ou une ouverture entre la plaque séparatrice (4) et la paroi latérale (3).
  7. Chambre d'échantillon selon la revendication 6, dans laquelle l'ouverture entre la plaque séparatrice (4) et la paroi latérale (3) est formée, au moins partiellement, par une encoche latérale dans la plaque séparatrice (4).
  8. Chambre d'échantillon selon la revendication 6 ou la revendication 7, dans laquelle l'ouverture entre la plaque séparatrice (4) et la paroi latérale (3) est formée, au moins en partie, par le fait que la plaque séparatrice (4) est espacée, au moins en partie, de la paroi latérale (3).
  9. Chambre d'échantillon selon l'une des revendications précédentes, dans laquelle le réservoir partiel supérieur et celui inférieur (6, 5) sont reliés par deux arrivées/sorties d'écoulement (7, 8), qui sont notamment agencées sur des côtés opposés du réservoir.
  10. Chambre d'échantillon selon l'une des revendications précédentes, dans laquelle le rapport de la surface de la plaque séparatrice (4) à la surface de base du réservoir est supérieur à 0,7, notamment supérieur à 0,8.
  11. Chambre d'échantillon selon l'une des revendications précédentes, dans laquelle le réservoir présente une surface de base de forme rectangulaire ou carrée.
  12. Chambre d'échantillon selon l'une des revendications précédentes, comprenant, en outre, un réservoir supplémentaire destiné à accueillir un échantillon, le réservoir supplémentaire étant délimité par la plaque de fond (2) et une paroi latérale (3), et une plaque séparatrice (4) parallèle à la plaque de fond (2), étant agencée dans le réservoir supplémentaire, chambre d'échantillon
    dans laquelle la plaque séparatrice (4), dans le réservoir supplémentaire, est agencée à une hauteur qui est inférieure à la hauteur minimale de la paroi latérale (3), de sorte qu'elle subdivise le réservoir supplémentaire en un réservoir partiel supérieur et inférieur,
    dans laquelle le réservoir partiel inférieur et le réservoir partiel supérieur sont délimités latéralement, en totalité, par la paroi latérale (3), et
    dans laquelle le réservoir partiel supérieur et celui inférieur (6, 5) sont reliés par au moins une arrivée/sortie d'écoulement (7, 8).
  13. Chambre d'échantillon selon la revendication 12, dans laquelle le réservoir et le réservoir supplémentaire présentent, en partie, une paroi latérale (3) commune.
  14. Chambre d'échantillon selon l'une des revendications précédentes, comprenant, en outre, un couvercle (9) pour fermer le réservoir, le couvercle (9) s'appliquant par contact de surface, notamment au moins en partie, sur la surface extérieure latérale de la paroi latérale (3).
  15. Chambre d'échantillon selon l'une des revendications précédentes, dans laquelle la surface intérieure du réservoir est au moins partiellement hydrophilisée.
  16. Chambre d'échantillon selon l'une des revendications précédentes, dans laquelle la plaque séparatrice est d'une configuration imperméable aux liquides, poreuse ou en forme de membrane.
EP11193029.3A 2011-12-12 2011-12-12 Chambre d'analyse dotée d'une plaque de séparation Active EP2604342B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DK11193029.3T DK2604342T3 (da) 2011-12-12 2011-12-12 Prøvekammer med skilleplade
EP11193029.3A EP2604342B1 (fr) 2011-12-12 2011-12-12 Chambre d'analyse dotée d'une plaque de séparation
US13/705,759 US9333503B2 (en) 2011-12-12 2012-12-05 Sample chamber with parting plate
CN201210536279.6A CN103157524B (zh) 2011-12-12 2012-12-12 具有隔板的样品室

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP11193029.3A EP2604342B1 (fr) 2011-12-12 2011-12-12 Chambre d'analyse dotée d'une plaque de séparation

Publications (2)

Publication Number Publication Date
EP2604342A1 EP2604342A1 (fr) 2013-06-19
EP2604342B1 true EP2604342B1 (fr) 2014-06-25

Family

ID=45418390

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11193029.3A Active EP2604342B1 (fr) 2011-12-12 2011-12-12 Chambre d'analyse dotée d'une plaque de séparation

Country Status (4)

Country Link
US (1) US9333503B2 (fr)
EP (1) EP2604342B1 (fr)
CN (1) CN103157524B (fr)
DK (1) DK2604342T3 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102792151B (zh) 2010-03-23 2015-11-25 加州理工学院 用于2d和3d成像的超分辨率光流体显微镜
US9643184B2 (en) * 2010-10-26 2017-05-09 California Institute Of Technology e-Petri dishes, devices, and systems having a light detector for sampling a sequence of sub-pixel shifted projection images
US9569664B2 (en) * 2010-10-26 2017-02-14 California Institute Of Technology Methods for rapid distinction between debris and growing cells
EP3095517B1 (fr) 2015-05-22 2020-01-08 ibidi GmbH Porte-échantillons ayant une structure de référence et procédé de production d'un porte-échantillons doté d'une structure de référence

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797259A (en) * 1986-12-15 1989-01-10 Pall Corporation Well-type diagnostic plate device
US4741619A (en) * 1987-05-05 1988-05-03 Molecular Devices Corporation Hydrophilic microplates for vertical beam photometry
CH687592A5 (de) 1993-10-18 1997-01-15 Eidgenoess Munitionsfab Thun Mehrgefaessanordnung zur Instrumental-Analyse.
US5795748A (en) * 1996-09-26 1998-08-18 Becton Dickinson And Company DNA microwell device and method
US6171780B1 (en) * 1997-06-02 2001-01-09 Aurora Biosciences Corporation Low fluorescence assay platforms and related methods for drug discovery
EP1262764B1 (fr) * 2001-05-25 2007-04-11 Corning Incorporated Procédé pour la détermination des réactions et de l'activité métabolique avec materiau fluorescent thermosensible
DE10148210B4 (de) 2001-09-28 2005-09-15 Ibidi Gmbh Flusskammer
EP1458483B1 (fr) * 2001-09-28 2014-01-08 ibidi GmbH Chambre d'ecoulement
WO2005079985A1 (fr) 2004-02-17 2005-09-01 Ibidi Gmbh Dispositif utilise pour effectuer des analyses microscopiques de fluides
ATE393693T1 (de) * 2004-03-22 2008-05-15 Ibidi Gmbh Verfahren zum flächigen quellschweissen eines kunststoffkörpers mit einem weiteren körper
EP2148921B1 (fr) * 2007-05-30 2017-07-19 Nikon Corporation Bac d'incubation
CA2678570C (fr) * 2008-09-12 2016-08-16 Stemcell Technologies Inc. Recipients pour la culture cellulaire pour la reduction de menisque avec des solutions aqueuses

Also Published As

Publication number Publication date
CN103157524A (zh) 2013-06-19
EP2604342A1 (fr) 2013-06-19
US20130171043A1 (en) 2013-07-04
CN103157524B (zh) 2016-03-02
DK2604342T3 (da) 2014-09-01
US9333503B2 (en) 2016-05-10

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