WO2009021488A1

WO2009021488A1 - Antioxidantcomplex based on grape vital (based on citrus seed and/or grape seed and/or flavonoid)

Info

Publication number: WO2009021488A1
Application number: PCT/DE2008/001276
Authority: WO
Inventors: Zsolt Csabai
Original assignee: Zsolt Csabai
Priority date: 2007-08-10
Filing date: 2008-08-07
Publication date: 2009-02-19
Also published as: DE112008002781A5; DE102007037772A1

Abstract

The invention relates to an antioxidant and stabilizer or preservative based on citrus fruit, grape seed and/or flavonoid and/or on bioflavonoid and various mixtures of superoxides. In addition, the invention further relates to an antioxidant complex of superoxide, diabase, catalase, glutathione peroxidase, melatin, epithalamin, Megamin, retinol (vitamin A), laetrile (vitamin B17), panganic acid (vitamin B16 and B6), and pangamic acid (vitamin B15), and carvediol CAE and a mixture with the antioxidant Grape Vital (grapefruit seed extract, hereinafter called GSE), which together form an antioxidant complex. The essential advantage of the antioxidant Grape Vital according to the invention which is citrus fruit based and/or flavonoid based and/or bioflavonoid based, in particular consists of a grapefruit flesh and/or GSE, and/or grape seed, is that, because of its complete harmlessness as a natural product, it may be used in the most varied sectors. In particular, the preservative according to the invention can be employed in the field of medicine and healing, of foods, cosmetics, water purification and animal husbandry.

Description

Antioxidant complex based on Grape Vital (citrus and / or grape seed and / or flavonoid base)

description

The invention relates to an antioxidant and stabilizer or citrus-based, grapeseed-based, and / or flavonoid-based and / or biofiavonoid-based and various mixtures of superoxides according to the preamble of claim 1.

The invention further relates to an antioxidant complex of superoxide, diabaz, catalaz, glutathione peroxidaz, melatin, epitalamine, megamin, retinol (vitamin A), lettrill (vitamin B ₇ ), panganic acid (vitamins B ₁₆ and B ₆ ), and pamgamic acid (vitamin B ₁₅ ), and carvediol CAE, and a mixture with the antioxidant Grape Vital (grapefruit seed extract, hereinafter referred to as GKE), which together form an antioxidant complex according to the preamble of claim 2.

This object is achieved by an antioxidant Grape Vital, having the features of claim 1-2.

The essential advantage of the inventive antioxidant Grape Vitals, which is based on citrus fruit and / or flavonoid and / or bioflavonoid based, in particular consists of a grapefruit meat and / or GKE, and / or grape seeds, is that it because of its complete harmlessness as a natural product in can be used in various fields. In particular, the preservative according to the invention in the field of medicine and medicine, food, cosmetics, water purification and livestock be applied, or in not yet mentioned areas and the previously known and og problematic chemical preservatives replaced. In this case, the present preservative may be mixed with the cosmetic products or even replace them.

Particularly advantageously, the antioxidant and preservative of the invention can be used in connection with the preservation of fruit, soft fruit, live animals, such as e.g. Oysters, clams, lobsters, etc., are used against fungal attack and bacterial attack during transport. The said animals are already fed prior to transport with the preservative of the invention in powder or liquid form, so that the innards are cleaned, or the fruit is simply sprayed with the present preservative.

The preservative according to the invention is also advantageous in animal breeding. The animals are fed with the preservative according to the invention in powder or in liquid form, the animals are freed from undesired bacteria, viruses, fungi and parasites, in chicken farms, e.g. The use of Salmonella disease et cetera, the animals are healthier, show more appetite, and thus they grow and thrive faster and better. It can also fully replace the antibiotics used in animal breeding. Diseases are not transmitted to humans!

Due to its pH, the present preservative can also be used to control pests, e.g. Aphids, spider mites et cetera, are used.

Advantageously, the present preservative is also used for water treatment, especially for drinking water treatment, where it serves as a full-fledged chlorine substitute. When using the present preservative between 40 - 300 ppm (part per million), an absolute bacteria and sterility in the treated water can be achieved. Water treatment in, for example, third countries or disaster areas is also of particular importance, with complete absence of germs being achievable without wastewater treatment plants. Tests have proven that it is also possible to treat salt water with the preservative according to the invention. Its use is not limited to freshwater.

Likewise, the present preservative can be used as a chlorine substitute in the swimming pool area and for general sterilization in the sauna, in the hospital area, dental area, and, for example, in the line area at dairies et cetera. In the field of dietary supplements and / or food, one can supply the GKE in sugar-free chewy candy and chewing gum. With about 5 sweets per day, the daily needs of a schoolchild can be covered, and thus protected against illness. The resistance, performance and the immune system are strengthened with it.

In the area of lakes, ponds and aquaristics, waterbed system, the entire plant world can benefit from the present preservative, since it can be used for the destruction of various types of algae. In the treatment of water in this area, fish no longer find a healthier water quality affected by parasites, have more appetite and show a greater colourfulness.

There are the most diverse chemical preservatives known, which are mostly toxic and their scope are therefore limited. For example, chemical preservatives can cause skin irritation and allergies. In addition, chemical products that negatively alter or even destroy the ingredients of emulsions and other cosmetic products.

The tests were successfully completed in cosmetic preparations such as O / W emulsion (low water content products), hydrogel and unalcoholic tonic. In these formulations, a microbiological aggression was simulated by typical microorganisms, from the production phase to the use of the cosmetic product. The tests showed a considerable preserving capacity against gram positive and gram negative bacteria. Thus, the product can be used in all cosmetic preparations, even in emulsions as a natural preservative and antioxidant and replace the harmful chemical preparations such as Bronopol, Paraossibenzoat et cetera and replace fully. These often cause irritation with the skin and often damage the other additives in the cosmetics. Thus, a cosmetic can only be preserved with Grape Vital.

The main advantage of the preservative according to the invention is that it has already been successfully tested in drinking water treatment. It should serve as a chlorine substitute in drinking water. With 40 drops of Grape Vital (GKE) you can make 1 liter of water germ-free. This means that in regions where there are no sewage treatment plants, or in flood- The drinking water can be made harmless drinkable without great effort before drinking. 250 liters of Grape Vital can prepare approx. 5 million liters of drinking water without the need for a sewage treatment plant. In flood disasters, people are supplied with relief supplies such as food, but people drink the water contaminated by E-CoIi (Latin: Escherichia coli, Salmonella and Streptococcus aureus bacteria responsible for cholera and other deadly diseases). Grape Vital has been proven to stop the multiplication of these bacteria after only 5 minutes.

The main advantage of the antioxidant according to the invention and stabilizer and preservative based on Grape Vital, that with 1 liter of Grape Vital, a swimming pool of 200 m ³ in private area, about 2-3 months absolutely can be dispensed with the use of chlorine. No Toxic Chlordämfe, no chlorine subsequent dosing required. Sauna can be sterilized without chemicals by natural means. The application area is so enormous.

The main advantage of the antioxidant according to the invention, stabilizer and preservative on Grape Vital basis, is that in the air conditioning technology is very often unfortunately found that when you turn off the air conditioning, an unpleasant moder odor comes from the plant. This is due to the rough surface structure of the air conditioning, in which the bacteria and fungi multiply. The destruction of bacteria and fungi solves the unpleasant problem.

The main advantage of the antioxidant according to the invention, stabilizers and preservatives based on Grape Vital, is that in agriculture, grapes, fruit trees, medicinal herbs and vegetables, sprayed with the highly toxic preparations against a variety of fungi, bacteria and pests. The tractors must be hermetically sealed, so that no consequential damage is caused to humans, due to self-poisoning. It can absorb the toxins through the respiratory system, the skin and hair. The Grape Vital Basis can be used safely against these.

The main advantage of the antioxidant according to the invention, stabilizer and preservative based on Grape Vital, is that irrigation systems can be kept sterile and algae-free in the agricultural industry. The essential advantage of the cleaning, sterilizing and preserving agent according to the invention based on Grape Vital, is that in the waterbed technique the beds can be kept sterile and algae-free.

The essential advantage of the inventive antioxidant, stabilizer and preservative based on Grape Vital, is that can be used in human medicine and in veterinary medicine, in the form of anti-fungal ointment.

The essential advantage of the antioxidant according to the invention, stabilizing and preserving agent based on Grape Vital, is that in the field of internal and external human medicine, the preservative of the invention is particularly advantageous for controlling bacteria and for treatment of fungal infections, parasite and virus infestation. This applies equally to the field of veterinary medicine. In particular, the use of the present preservative is recommended where animals are kept in a confined space without adequate freedom of movement. An example of this are chicken laying batteries. There is a high rate of Salmonella disease affecting not only the eggs but also the meat. Through the use of the present agent is also a high rate of death and infection, even outbreaks of epidemics preventable.

The essential advantage of the antioxidant according to the invention, stabilizing agent and preservative based on Grape Vital, is that it can be used in human medicine as a herbal ointment.

The essential advantage of the antioxidant according to the invention, stabilizer and preservative based on Grape Vital, is that can be used in human medicine as anti-dandruff, when used against psoriasis and psoriasis, acne treatment, skin creams, aftershaves, and shower gels and as aerosol spray, patches which cleanses the foot and foot paving.

The essential advantage of the antioxidant according to the invention, stabilizing agent and preservative based on Grape Vital, is that it can be used successfully in human medicine as a wound healing agent in dissolved form, but also in cases of burns and blood poisoning. The main advantage of the Grape Vital based antioxidant according to the invention is that it can be used in human medicine for oral or for external use.

The essential advantage of the antioxidant according to the invention on a Grape Vital basis, is that it can be used in human medicine as Scheidenzäpchen, against fungal, bacterial, viral and parasitic infections and. Tests are already being successful in the treatment of Candida and human papiloma virus (HPV), hepatitis C and other viral types of carcinogens such as MDR, JHI, CaCo2, MG-63, HelLaS3, HAT-29, A-549 and MMT.

The main advantage of the antioxidant according to the invention on Grape Vital basis, is that in human medicine experiments have shown that after 2 weeks of taking the number of phagocytes p53 increased by 95% fold. The p53, HA-ras, Bcl2, HER2 and K-ras and c-myc gene, are gene expression inhibitors produced by the action of the carcinogens and the repair of the DNA in the tested internal organs (liver, heart, lung, kidney). The p53 Gen Suppressor has been shown to damage the cell cycle, the so-called Alphal (Gl) phase, which stops cell division in order to save time in the so-called DNA repair phase. If the selfrepair fails, the genetically modified cell or gene is driven into an apopptic dose or ulcer cell death by the addition of other genes.

The main advantage of the antioxidant according to the invention based on Grape Vital, is that in human medicine experiments have shown that the effect of the antioxidant in certain amounts in the body, proven to prevent more than 100 health changes, such as heart and blood vessel diseases (heart attacks, vessel high blood pressure), circulatory disorders in the brain, cataracts and allergies, and the dangers and risks of cancer, as well as slowing down the body's aging process. It protects the body from the expiration, which is called oxidation. Oxigen is vital to the body, but through oxidation, our body is constantly exposed to attacks by the freeflower. These are unstable molecules that pull the electrons away from other molecules, and damage our bodies through the cellular level. Here is the most obvious the fat cell damage or lipid peroxidation. The free-root also attack the nucleic acid base (adenine, timin, guanine, and citrine: the primordial component of DNA), thus preventing perfect duplication.

The essential advantage of the antioxidant according to the invention based on Grape Vital, is that in human medicine experiments have shown that the stabilized oxygen reaches the organism in molecular form through the food into the bloodstream. The stabilized oxygen can be easily utilized by the body, is extremely stable, does not degrade even after years. Due to the industrialization and the associated pollution of the oxygen content of the air is less and less and therefore a constantly decreasing oxygen content is observed. The smog and poor air quality in cities and industrial areas, such as Düsseldorf-Cologne, pronounced strong industrial cities, where the organism is no longer a sufficient source of oxygen. The higher load and requirement on the organism in turn requires more oxygen.

The stabilized oxygen supports the oxygen supply to the cells, the physiological processes are improved. The body is charged with energy. Has a positive effect on the metabolism and blood circulation. People who suffer from asthma and shortness of breath, help with the respiratory problems resulting symptoms. The organism can more easily utilize the utilization of nutrients such as vitamins, minerals, amino acids and medicinal plants. The body is more powerful and more resilient.

When used in HIV disease, where the already weakened body is easily weakened by infection even more, which can lead to death, the present agent strengthens the immune system.

The object of the present invention is therefore to provide a cleaning, sterilizing and preservative that is completely compatible with humans and animals and plants, both on land and in water, harmless, therefore, can be applied at any time and arbitrarily.

The main advantage of the Grape Vital based antioxidant according to the invention is that it can be used as a natural preservative in food preservation and stabilization. The main advantage of the antioxidant according to the invention based on Grape Vital, is that it can be used in cosmetics as a natural preservative.

The main advantage of the antioxidant according to the invention on Grape Vital basis, is that it can be used in dentistry as a toothpaste or gel, as sugarless chewing gum and as chewing candy, and thus the daily needs, especially in children can administer.

Further advantageous embodiments and areas of application of the present invention will become apparent from the dependent claims.

The present invention is based on the finding that a concentrate of citrus pulp and / or citrus seed extract as a natural product or a corresponding synthetically produced agent excellent as a preservative, in particular for the preservation of cosmetic products and emulsions, food, fruit, live animals, as Antioxidants, for water treatment, sterilization, for the treatment of bacteria, fungal infections, parasitic and viral infestation in the field of human and veterinary medicine and for algae control in lakes, ponds and aquarium use.

The chemical formula for these inventive antioxidants Grape Vital is diphenolbenzene complex and bio-flavonoid complex.

The present preservative can be presented as a liquid agent, in powder form, in capsules, suppositories or tablet form.

Citrus fruits of all kinds, in particular grapefruit, lemons, limes, oranges, mandarins, etc., are suitable for obtaining the present preservative as a natural product. Any type of extraction of the pulp and / or a nuclear extract from these fruits is conceivable. Flavonoids are active substances of grapefruit. They occur in about 10,000 plants and are among the so-called secondary metabolites of the plant metabolism. The bio-flavonoids are the main ingredients of the nuclear extract or the final product. Bio-flavonoids or bio-flavonoid complexes are compounds which, biologically speaking, are responsible for the high effectiveness of the present preservative. Used in cosmetics, bio-flavonoids have an activating and regenerating effect on the skin. They are natural antioxidants and can scavenge the harmful free radicals; they are thus radical scavengers. Free radicals are very aggressive fission products of various natural and synthetic substances. They are usually caused by reactions by or from oxygen, light and heat, which are generated from environmentally relevant chemicals, ozone, solvents, dyes, etc. These radicals react easily with fats, proteins and genetic material. They can destroy the cell walls and thus also the most important functions of the cells. They are also demonstrably involved in the development of cancer. A protective effect can be provided by the so-called antioxidants, which can intercept and inactivate the free radicals.

Some effective flavonoids and their active ingredients are: Epicatechin (EC), Gallocatechin (GC), Epigallocatechin (EGC), Epicatechin-3-gallate (ECG), Epigallocatechin-3-gallate (EGCg), Catechins, Hesperdin, Naringin, Quercetin.

In the following, a preferred method for producing the grapefruit-based preservative will be explained.

1. For the production of whole fruits, preferably from controlled cultivation are used. This ensures that the fruits are not spoiled or come from any crop that is contaminated by pesticides or cyanides. It must also be ensured that the pressed residue from the Grapefruitsaftgewinnung is not mixed with chemicals. The fruits must not be obtained from the waste products of grapefruit juice production as a raw material, as this raw material must be preserved without immediate further processing by preservatives. There is therefore the problem that this raw material is useless for use in the field of humans and animals.

2. The grapefruit is separated into pulp and seeds. 3. The pulp and kernels are then dried in a gentle physical process. This can be done by freeze-drying or by heating. When drying by heat must be between 130 ° C and 225 ⁰ C, dried for 20 to 50 hours. This depends on the heat and the degree of moisture of the fruit or the raw material.

4. The concentration of the final product depends on the ratio of pulp to kernel proportions and whether the kernels are mixed between the citrus fruits. This is possible, however, the effect of the final product is not so high. Preferably 15% pulp is mixed with 85% kernels, but this always varies according to the required quality between 100% pulp and 100% kernels.

5. This mixture is then added between 5% to 100% by weight of pure vegetable 99.6% pharmaceutical glycerol. The use of animal glycerin should not be used because of the purity and danger of the source and BSE for safety reasons. It must not be used in the production of synthetic chemical aids. Due to the careful production no chemical change takes place and thus the ingredients are not damaged and remain unchanged.

6. The total mixture is then heated to 125 ° C to 160 ° C and stirred for 2.5 to 5 hours under 3 - 5 bar pressure. Glycerine is the extraction or extraction medium. This time is enough to extract the ingredients from the base product.

7. Through a circulation that leads to the outside via a magnet system, the iron-containing metal particles are filtered out. Likewise, during this circulation, a region of ultraviolet light flows through to stabilize the components in the product.

8. This mixture then cools down to about 50 ^° C. and the ingredients, such as ascorbic acid (vitamin C) are added, depending on the recipe, between 5% -20%. It must not be used synthetically produced ascorbic acid, since here the natural Life is maximum 8 weeks. For ascorbic acid on a natural basis, this is not the case.

9. The basic product has an approximately 15% bio-flavonoid content. In order to increase the efficiency of the final product even further, between 10% to 45% of bio-flavonoids can be added to the mixture. Flavonoids occur in about 10,000 plants and are among the so-called secondary metabolites of the plant metabolism. The bio-flavonoids are the main ingredients of the nuclear extract or the final product.

10. The new mixture is gently stirred for approx. 0.5 - 2 hours. The end result is a syrup with a nice yellow, amber base color. This indicates a natural production. A chemically or synthetically produced product has more of a neutral color and must be colored additionally. Here only lead-free food colors may be used.

11. After cooling, the syrup is filtered through a 250-400 mesh screen to obtain a high viscosity liquid. Filtered can be up to 120 bar in the cold state of the syrup by pressing on physical ways.

12. The pH is now 2.5 - 3.0.

In the following two examples are given of compositions of a liquid grapefruit extract and a grapefruit extract in powder and tablet form:

Liquid Grape Vital

Active ingredients:

GKE, nitrogen-free extract 45.0%

Ascorbic acid, vitamin C 16.3%

Protein (min) N = 0.32 x 6.25 2.0%

Fat 1.0% Inert ingredients:

Glycerine U.S.P. 29.9%

Mineral ash (max.) 0.4%

Fibers (max.) 0.4%

Humidity (max.) 5.0%

Total 100.0%

Powdered grapefruit extract and tablets and capsules

Active ingredients:

Antioxidants Grape Vital, non-volatile extract 45.0% ascorbic acid, vitamin C (max.) 16.5%

Protein (min) N = 0.32 x 6.25 2.0%

Fat 1.0%

Inert ingredients:

Sipernat 22S, Silicon Dioxide - U.S.P. 17.0%

Glycerine U.S.P. 17.6%

Mineral ash (max.) 0.5%

Fibers (max.) 0.4%

Total 100.0%

Scheidungäpchen from antioxidants Grape Vital Liquid or powder

Active ingredients:

Antioxidant Grape Vital Powder 1 - 300 mg

Or

Antioxidants Grape Vital Liquid 0.1 - 15 tropics

Rest of cocoa butter

The products are free of: benzethonium chlorides, benzalkonium chlorides, pesticides, cyanides and alcohol. Depending on the application, the present Citrusfruchtfleisch- bez. Citrus fruit kernel extracts in any representations or formulations and concentrations applicable. A particular advantage is that the said extracts are not subject to the drug law, because they are non-toxic as natural products. They are to be classified as nutritional supplements or medicinal herbs. The extracts mentioned, especially grapefruit extracts, destroy not only viruses and bacteria, but also fungi, such as yeasts, and parasites. The effect therefore covers areas of antibiotics (antibacterial), antifungals (fungicides), antivirals (virucidal) and antiparasitics (destruction of unicellular parasites), all of which are met by a single product. The present preservative effectively destroys pathogens, without weakening the immune system and without destroying the intestinal flora. All associated negative consequences can therefore be avoided.

In particular, the present antioxidant and preservative in the fields of pharmacies, hospitals, pharmaceuticals, cosmetics, fine chemicals, food, cosmetics, animal husbandry, aquarium and pond technology, swimming pool and sauna technology, dentists, dental laboratories, water treatment (disaster areas), dental care and hygiene, apply ,

The following table shows the list of bacteria, fungi and yeasts that are particularly well combated with the present preservative.

Physical properties

Chemical Description Diphenol benzene complex Appearance (liquid) Liquid / Heavy Viscous

Color (Gardner) Lemon Yellow

Odor Mild citrus

Specific gravity (25 ° C) 1,110

Density (lbs./gal.) 9.68 pH (25 ° C) 2.0-3.0

Flash Point (° C / ° F) 148.9 / 292

Viscosity (Centistoke) 134.91

Viscosity index -23.2

Surface Tension, Average of (5) readings (dynes / cm) 40.0 Apparent Interfacial Tension (dynes / cm) 5.0

True Corrected Interfacial Tension (dynes / cm) 4,5

Molecular Weight 565

Solubility Water, Alcohol & organic solvents

The invention furthermore relates to an antioxidant complex of superoxide, diabaz, catalaz, glutathione peroxidaz, melatin, epitalamine, megamine, retinol (vitamin A), lettrill (vitamin B 17), panganic acid (vitamin B 16), and pamgamic acid (vitamin B 15), and Carvediol CAE, and a mixture with the antioxidant Grape Vital (grapefruit seed extract, hereinafter referred to as GKE), which together form an antioxidant complex according to the preamble of claim 17.

This object is achieved by an antioxidant complex based on Grape Vital, with the features of claim 17.

The main advantage of the Grape Vital based antioxidant complex of the invention is that in genetic engineering experiments have shown that the p53, HA-ras, Bcl2, HER2 and K-ras and c-myc gene, the cell cycle, more than that 10 times, that is to say at the same time, in which 21 cell divisions were so far, the number of cell divisions increases to 260 in the same time. This is extremely important for diabetics who are slowing the healing process. By taking advantage of the Selfrepair phase, the genes are switched on and / or put back on an information basis of approximately 35 years. High doses of the antioxidant complex protect the DNA from oxidation, resulting in a slower aging process. The loss of genetic information has been reduced to a minimum.

The main advantage of the antioxidant complex according to the invention based on Grape Vital, is that by adding the carvediol CAE and epitalamine, the heart is strengthened, this is enormously important after a heart attack in the recovery phase.

The invention further relates to an addition to an antioxidant complex of superoxide dismutase SOD, the enzyme superoxide dismutase catalyses the conversion of superoxide radicals to oxygen and hydrogen peroxide. This is a disproportionation reaction. "Dismutation". SOD

2 O ^* - + 2H ¹ H ₂ O ₂ + O.

SOD catalyzed reaction

The hydrogen peroxide formed is cytotoxic and is disproportionated via catalases to oxygen and water or the enzymes peroxidases and haloperoxidases otherwise utilize the hydrogen peroxide. Superoxide radicals must be eliminated as quickly as possible in the body because they are cytotoxic and can withdraw electrons from other molecules. Superoxide radicals are very short-lived (half-life of 10 ^-6 s) and can deprive electrons from other molecules, and the resulting free radicals initiate a kind of chain reaction. "Biomolecules are basically very sensitive to oxidative damage caused by free radicals oxidative cell metabolism takes place in the mitochondria, which causes the most damage to lipids, proteins, and DNA, which are important for the decomposition of superoxide radicals, oxygen-affine metal ions whose valence can easily change, and SOD must be resistant to the aggressive radical anion. Other O ₂ -activating centers (heme systems, type 3 copper proteins, non-heme dimers, copper / cofactor complexes, cytochrome c oxidase) contain at least two interacting, redox-active centers to facilitate the one-electron reduction of O ₂ to O ₂ ^" to get around. The hydrogen peroxide formed in the reaction is also cytotoxic and is disproportionated via catalases to oxygen and water. Other enzymes such as peroxidases and haloperoxidases can also break down the hydrogen peroxide.

The invention further relates to an additive to an antioxidant Koplexes from Katalaz and glutathione peroxidaz, which is indeed produced by the body, but not in the necessary amounts. It is an endogenous antioxidant that has iron. The Katalaz breaks down hydrogen peroxide.

The invention further relates to an addition to a Antioxidantenkoplexes of diabaz, me- latin and megamine, these are dietary supplements of volcanic earth, which consists of zeolite, ie purely natural substances. The regular preventive consumption of megamine complexes Tribo-activated zeolite helps to maintain the long-term performance. In addition, cell-damaging oxidation processes in the body are inhibited. Free radicals have no chance.

The invention further relates to an addition to an antioxidant Koplexes of vitamin mixture, retinol (vitamin A), Leatrill (vitamin B 17), Pangansäure (vitamins B16 and B6), and Pamgamic acid (vitamin B 15).

The panganic acid vitamin B16 or phosphorylated vitamin B6 derivatives act as coenzyme in about 100 enzymatic reactions. Almost all reactions take place in the amino acid metabolism. Another important role is played by pyridoxal phosphate (PLP or PALP) (a pyridoxine derivative) as a cofactor in the synthesis of Δ ^ aminolevulinic acid, an intermediate in endogenous heme synthesis. The participation of pyridoxal phosphate as a cofactor in the degradation of animal starch (glycogen) may also be mentioned.

The following tables show the listing of bacteria, fungi and yeasts, viruses and unicellular parasites that are particularly well combated with the present preservative.

GRAPE VITAL ® "" Minimum Inhibitory in vitro (MIC)

Gram-positive bacteria Origin and Strain no. MIC (ppm)

Bacillus subtilis NCTC 8236 2

Bacillus megatherium A 60

Bacillus cereus A 60

Bacillus cereus var mycoides A 60

Clostridium botulinum NCTC 3805 60

Clostridium tetani NCTC 9571 60

Corynebacterium acnes ATCC 6919 60

Corynebacterium diphtheriae ATCC 6917 60

Corynebacterium diphtheriae NCTC 3984 60

Corynebacterium diphtheriae A 60

Corynebacterium minutissium ATCC 6501 100

Diplococcus pneumoniae NCTC 7465 60

Lactobacillus arabinosus CITM 707 66

Lactobacillus arabinosus ATCC 8014 66

Lactobacillus casei CITM 707 100 Listeria monocytogenes ATCC 15313 20 Mycobacterium tuberculosis A 2000 Mycobacterium smegmatis NCTC 8152 20 Mycobacterium phlei A 6 Sarcina lutea NCTC 196 60 Sarcina ureae ATCC 6473 2 Staphylococcus aureas NCTC 7447 2 Staphylococcus aureas NCTC 4163 2 Staphylococcus aureas NCTC 6571 6 Staphylococcus aureas NCTC 6966 2 Staphylococcus aureas ATCC 13709 2 Staphylococcus aureas ATCC 6538 2 Staphylococcus albus NCTC 7292 2 Staphylococcus albus CG. 6 Streptococcus agalactiae NCTC 8181 60 Streptococcus haemoyticus AA 20 Streptococcus faecalis NCTC 8619 200 Streptococcus faecalis ATCC 10541 60 Streptococcus pyogenes NCTC 8322 60 Streptococcus viridans 20

Gram-negative bacteria Origin and Strain no. MIC (ppm)

Aerobacter aerogenes CITM 413 20 Alcalingenes faecalis A 2000 Brucella intermedia A 2 Brucella abortus NCTC 8226 2 Brucella melitensis A 2 Brucella suis A 2 Cloaca cloacae NCTC 8155 6 Escherichia coli NCTC 86 2 Escherichia coli ATCC 9663 6 Escherichia coli ATCC 11229 16 Escherichia coli NCTC 9001 6 Haemophilus influenzae A 660 Klebsiella edwardsii NCTC 7242 6 Klebsiella aerogenes NCTC 8172 6 Klebsiella pneumoniae ATCC 4352 6 Legionella pneumoniae isolate 200 Loefflerella mallei NCTC 9674 6 Loefflerella pseudomallei NCIB 10230 20 Moraxella duplex A 2 Moraxella glucidolytica A 6 Neisseria catarrhalis NCTC 3622 660 Pseudomonas aeruginosa ATCC 15442 250 Pseudomonas capacia C-175 5000 Pasteurella septica NCTC 948 2 Pasteurella pseudotuberculosis CG. 200 Proteus vulgaris NCTC 8313 2 Proteus mirabilis A 6

Pseudomonas aeruginosa NCTC 1999 2000

Pseudomonas aeruginosa ATCC 12055 20,000

Pseudomonas fluorescens NCTC 4755 2000

Salmonella choleraesuis 50

Salmonella choleraesuis ATCCl 0708 660

Salmonella enteritidis A 6

Salmonella gallinarum 50

Salmonella typhimurium NCTC 5710 6

Salmonella typhi NCTC 8384 6

Salmonella paratyphi A NCTC 5322 6

Salmonella parathypi B NCTC 3176 6

Salmonella pullorum ATCC 9120 6

Serratia marcescens A 2000

Shigella flexneri NCTC 8192 6

Shigella sonnei NCTC 7240 3

Shigella dysenteriae NCTC 2249 2

Vibrio cholerae A 200

Vibrio eltor NCTC 8457 200

Claims

Antioxidant complex based on Grape Vital (citrus and / or grape seed and / or flavonoid) claims

1. Antioxidant Grape Vital Citrus-based and / or flavonoid-based and / or grape-seed based preservatives, consisting of a citrus pulp and / or citrus kernel extract or chemically produced diphenolbenzene complex.

2. Antioxidant Grape Vital according to claim 1, characterized in that grapefruit, lemons, limes, oranges, tangerines, grape seeds, peach kernel, plum kernel, apricot kernel, almonds, etc. are used as citrus fruit.

3. Antioxidant Grape Vital according to claim 1 and 2, characterized in that the meat and / or nuclear extract glycerol or the like is added as a carrier.

4. Antioxidant Grape Vital according to claims 1 to 3, characterized in that it is in liquid or powder form, jelly capsules or in capsules, in the form of tablets, chewy and lollipop, chewing gum, or as a suppository.

5. Application of the antioxidant Grape Vital according to claims 1 to 4, characterized in that it is used for the treatment of bacteria, fungi, parasites and viruses.

6. Application of the antioxidant Grape Vital according to claim 5, characterized in that it is used for the preservation of food and cosmetics and dentistry and to replace the chemical preservatives.

7. Application of the antioxidant Grape Vital according to claim 5, characterized in that it is used for preserving fruit, vegetables or live animals by spraying.

8. Application of the antioxidant Grape Vital according to claim 5, characterized in that it is used for cleaning and keeping animals clean in animal husbandry, both for internal and for external treatment.

9. Application of the antioxidant Grape Vital according to claim 5, characterized in that it is used for cleaning and sterilization in medicine, both in human medicine and in veterinary medicine.

10. Application of the antioxidant Grape Vital according to claim 5, characterized in that it is used for water treatment in particular for drinking water treatment, for water treatment in the swimming pool area, in the area of lakes, ponds and in the field of A- quaristik.

11. Process for the preparation of a preservative according to claims 1 to 5, characterized in that a) the citrus fruits are separated into pulp and kernels, b) the pulp and the kernels are dried, c) a predetermined mixture of dried pulp and dried kernels (d) admixing between 5% to 100% by weight of glycerin; e) heating the total mixture to about 120 ° C to 165 ° C for about 2.5 to 5 hours under about 3-5 bar pressure, and f) the Total mixture is cooled.

12. The method according to claim 11, characterized in that between the steps e) and f) with a magnetic system iron-containing metal particles are removed from the overall mixture.

13. The method according to claim 11 and 12, characterized in that between the steps e) and f), the total mixture is irradiated with UV light.

14. The method according to claim 11 to 13, characterized in that the total mixture after step f) ascorbic acid between about 1% to 35% by weight are added.

15. The method according to claim 11 to 14, characterized in that the total mixture after step f) between about 0.01% to 95% by weight of bio-flavonoids are added.

16. The method according to claim 15, characterized in that the mixture is stirred for about 0.5 to 2 hours.

17. Antioxidant complex Grape Vital Citrus-based and / or flavonoid-based and / or grape-seed based preservatives, consisting of a citrus pulp and / or citrus kernel extract or chemically produced diphenolbenzene complex.

18. Antioxidant complex Grape Vital according to claim 17, characterized in that grapefruit, lemons, limes, oranges, tangerines, grape seeds, peach kernel, plum kernel, apricot kernel, almonds, etc. are used as citrus fruit.

19. Antioxidantkomplex Grape Vital according to claim 17 and 18, characterized in that the meat and / or nuclear extract glycerol or the like is added as a carrier.

20. Citrus-based and / or flavonoid-based and / or grapeseed-based Grape Vital-based antioxidant complex, wherein it consists of a citrus pulp and / or citrus kernel extract or of chemically produced diphenolbenzene complex.

21. The antioxidant complex based on Grape Vital according to claims 17 to 20, characterized in that superoxide, diabaz, catalaz, glutathione peroxidaz, melatin, epitalamin, megamin, retinol (vitamin A), lettrill (vitamin B 17), panganic acid ( Vitamin B 16), and pamgamic acid (vitamin B 15), carvediol CAE and folic acid in a value of 0.1 μg - 500 mg. This can refer to per kg / body.

22. Use of the antioxidant complex based on Grape Vital according to claims 17-21, characterized in that it serves to regenerate the DNA and strengthen the entire immune system.

23. Application of the Grape Vital based antioxidant complex according to claim 17-21, characterized in that it is used for controlling ulcers and cancer.

24. Use of the antioxidant complex based on Grape Vital according to claim 17-21, characterized in that it is used to strengthen the heart and Kreislausystemes.