WO2008153223A1 - 核酸と被験物質との結合親和性を測定するための組成物及びその利用 - Google Patents

核酸と被験物質との結合親和性を測定するための組成物及びその利用 Download PDF

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Publication number
WO2008153223A1
WO2008153223A1 PCT/JP2008/063705 JP2008063705W WO2008153223A1 WO 2008153223 A1 WO2008153223 A1 WO 2008153223A1 JP 2008063705 W JP2008063705 W JP 2008063705W WO 2008153223 A1 WO2008153223 A1 WO 2008153223A1
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WO
WIPO (PCT)
Prior art keywords
substance
nucleic acid
binding affinity
measuring
composition
Prior art date
Application number
PCT/JP2008/063705
Other languages
English (en)
French (fr)
Inventor
Kazuhiko Nakatani
Jinhua Zhang
Shiori Umemoto
Shinichi Sasaoka
Takahiro Wazaki
Original Assignee
Osaka University
Nitto Kasei Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Osaka University, Nitto Kasei Co., Ltd. filed Critical Osaka University
Priority to CA2694030A priority Critical patent/CA2694030A1/en
Priority to EP08791936A priority patent/EP2175275A4/en
Priority to US12/452,878 priority patent/US20110263041A1/en
Priority to JP2009519347A priority patent/JP4533981B2/ja
Publication of WO2008153223A1 publication Critical patent/WO2008153223A1/ja
Priority to US13/484,505 priority patent/US8741659B2/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

　本発明に係る核酸と被験物質との結合親和性を測定するための組成物は、ＲＮＡに結合可能であって、かつ、ＲＮＡから遊離しているときの蛍光強度が、ＲＮＡに結合しているときの蛍光強度よりも強い有機蛍光体を含む。これにより、被験物質と核酸との結合親和性を測定するに際して、高精度かつ簡便に測定することができ、さらに、被験物質として様々な物質を利用可能とする。
PCT/JP2008/063705 2007-07-31 2008-07-30 核酸と被験物質との結合親和性を測定するための組成物及びその利用 WO2008153223A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA2694030A CA2694030A1 (en) 2007-07-31 2008-07-30 Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof
EP08791936A EP2175275A4 (en) 2007-07-31 2008-07-30 COMPOSITION FOR MEASURING BINDING SAFFINITY BETWEEN NUCLEIC ACID AND SUBSTANCE AND THEIR USE
US12/452,878 US20110263041A1 (en) 2007-07-31 2008-07-30 Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof
JP2009519347A JP4533981B2 (ja) 2007-07-31 2008-07-30 核酸と被験物質との結合親和性を測定するための組成物及びその利用
US13/484,505 US8741659B2 (en) 2007-07-31 2012-05-31 Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007199970 2007-07-31
JP2007-199970 2007-07-31

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/452,878 A-371-Of-International US20110263041A1 (en) 2007-07-31 2008-07-30 Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof
US13/484,505 Division US8741659B2 (en) 2007-07-31 2012-05-31 Composition for measuring the binding affinity between nucleic acid and test substance, and use thereof

Publications (1)

Publication Number Publication Date
WO2008153223A1 true WO2008153223A1 (ja) 2008-12-18

Family

ID=40129817

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/063705 WO2008153223A1 (ja) 2007-07-31 2008-07-30 核酸と被験物質との結合親和性を測定するための組成物及びその利用

Country Status (5)

Country Link
US (2) US20110263041A1 (ja)
EP (2) EP2175275A4 (ja)
JP (1) JP4533981B2 (ja)
CA (1) CA2694030A1 (ja)
WO (1) WO2008153223A1 (ja)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4533981B2 (ja) 2007-07-31 2010-09-01 国立大学法人大阪大学 核酸と被験物質との結合親和性を測定するための組成物及びその利用
WO2015061573A1 (en) * 2013-10-23 2015-04-30 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Compounds that bind to human immunodeficiency virus rev response element

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2568699B2 (ja) * 1989-07-14 1997-01-08 松下電器産業株式会社 免疫的検出方法
JP2002241397A (ja) * 2001-02-20 2002-08-28 Rikogaku Shinkokai 蛍光性蛋白質および薬物探索法
JP2003334078A (ja) * 1999-04-20 2003-11-25 National Institute Of Advanced Industrial & Technology 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法

Family Cites Families (14)

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CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5151507A (en) 1986-07-02 1992-09-29 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5242796A (en) 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US5654442A (en) 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
WO1994005688A1 (en) 1992-09-03 1994-03-17 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
JP3927630B2 (ja) 1996-09-27 2007-06-13 エーザイ・アール・アンド・ディー・マネジメント株式会社 ウイルス感染症の予防・治療剤
DE60045059D1 (de) 1999-04-20 2010-11-18 Nat Inst Of Advanced Ind Scien Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten
US6448407B1 (en) 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
AU2002367810A1 (en) * 2002-03-28 2003-11-10 Rutgers, The State Of University Of New Jersey Bis-transition-metal-chelate-probes
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US7365193B2 (en) 2004-02-04 2008-04-29 Abbott Laboratories Amino-substituted tricyclic derivatives and methods of use
JP4533981B2 (ja) 2007-07-31 2010-09-01 国立大学法人大阪大学 核酸と被験物質との結合親和性を測定するための組成物及びその利用

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2568699B2 (ja) * 1989-07-14 1997-01-08 松下電器産業株式会社 免疫的検出方法
JP2003334078A (ja) * 1999-04-20 2003-11-25 National Institute Of Advanced Industrial & Technology 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法
JP2002241397A (ja) * 2001-02-20 2002-08-28 Rikogaku Shinkokai 蛍光性蛋白質および薬物探索法

Non-Patent Citations (3)

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Title
OH K.J ET AL.: "Excimer-Based Peptide Beacons: A Conventional Experimental Approach for Monitoring Polypeptide-Protein and Polypeptide-Oligonucleotide Interactions", J. AM. CHEM. SOC., vol. 128, no. 43, 2006, pages 14018 - 14019, XP008128279 *
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PAINTER G.R. ET AL.: "Initial Binding of 2'-Deoxynucleoside 5'-Triphosphates to Human Immunodeficiency Virus Type 1 Reverse Transcriptase", J. BIOL. CHEM., vol. 266, no. 29, 1991, pages 19362 - 19368, XP008128276 *

Also Published As

Publication number Publication date
US20110263041A1 (en) 2011-10-27
JP4533981B2 (ja) 2010-09-01
CA2694030A1 (en) 2008-12-18
US20120238029A1 (en) 2012-09-20
US8741659B2 (en) 2014-06-03
EP2175275A1 (en) 2010-04-14
EP2482076A1 (en) 2012-08-01
EP2175275A4 (en) 2010-09-01
JPWO2008153223A1 (ja) 2010-08-26

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