WO2008136263A1 - 凍結しないdnaリガーゼ反応組成物 - Google Patents
凍結しないdnaリガーゼ反応組成物 Download PDFInfo
- Publication number
- WO2008136263A1 WO2008136263A1 PCT/JP2008/057411 JP2008057411W WO2008136263A1 WO 2008136263 A1 WO2008136263 A1 WO 2008136263A1 JP 2008057411 W JP2008057411 W JP 2008057411W WO 2008136263 A1 WO2008136263 A1 WO 2008136263A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- composition
- dna ligase
- unfreezable
- reaction composition
- ligase reaction
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
【課題】本発明の目的は、添加剤を検討し、-30°C保存で少なくとも一週間凍結しないリガーゼ組成物、および使用方法を提供することである。また、それらを含有する試薬・キットおよび製造法を提供することである。 【解決手段】-30°C保存で少なくとも1週間凍結しない、DNAリガーゼ反応組成物であって、好ましくはベタイン及びグリセロールを含有する組成物。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007-118426 | 2007-04-27 | ||
JP2007118426A JP2008271834A (ja) | 2007-04-27 | 2007-04-27 | 凍結しないdnaリガーゼ反応組成物 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2008136263A1 true WO2008136263A1 (ja) | 2008-11-13 |
Family
ID=39943380
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2008/057411 WO2008136263A1 (ja) | 2007-04-27 | 2008-04-16 | 凍結しないdnaリガーゼ反応組成物 |
Country Status (2)
Country | Link |
---|---|
JP (1) | JP2008271834A (ja) |
WO (1) | WO2008136263A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014517688A (ja) * | 2011-05-06 | 2014-07-24 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | ライゲーションの促進 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01132362A (ja) * | 1987-11-19 | 1989-05-24 | Taiyo Fishery Co Ltd | 非凍結保存法 |
JPH0423987A (ja) * | 1990-05-16 | 1992-01-28 | Toyobo Co Ltd | 安定化された制限酵素SfiI組成物 |
JPH07194378A (ja) * | 1993-12-28 | 1995-08-01 | Fuji Seito Kk | トレハロースによる酵素の安定化法 |
JP2002034558A (ja) * | 2000-07-21 | 2002-02-05 | Nof Corp | 安定化された蛋白質分解酵素およびその用途 |
JP2006025637A (ja) * | 2004-07-13 | 2006-02-02 | Toyobo Co Ltd | Dnaリガーゼによるライゲーション反応の促進方法およびdnaリガーゼ組成物 |
-
2007
- 2007-04-27 JP JP2007118426A patent/JP2008271834A/ja active Pending
-
2008
- 2008-04-16 WO PCT/JP2008/057411 patent/WO2008136263A1/ja active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01132362A (ja) * | 1987-11-19 | 1989-05-24 | Taiyo Fishery Co Ltd | 非凍結保存法 |
JPH0423987A (ja) * | 1990-05-16 | 1992-01-28 | Toyobo Co Ltd | 安定化された制限酵素SfiI組成物 |
JPH07194378A (ja) * | 1993-12-28 | 1995-08-01 | Fuji Seito Kk | トレハロースによる酵素の安定化法 |
JP2002034558A (ja) * | 2000-07-21 | 2002-02-05 | Nof Corp | 安定化された蛋白質分解酵素およびその用途 |
JP2006025637A (ja) * | 2004-07-13 | 2006-02-02 | Toyobo Co Ltd | Dnaリガーゼによるライゲーション反応の促進方法およびdnaリガーゼ組成物 |
Non-Patent Citations (1)
Title |
---|
"TaKaRa Bio Sogo Catalog 2002-2003", 2002, pages A-63 - A-64 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014517688A (ja) * | 2011-05-06 | 2014-07-24 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | ライゲーションの促進 |
Also Published As
Publication number | Publication date |
---|---|
JP2008271834A (ja) | 2008-11-13 |
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