WO2008136263A1 - 凍結しないｄｎａリガーゼ反応組成物 - Google Patents

凍結しないｄｎａリガーゼ反応組成物 Download PDF

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Publication number
WO2008136263A1
WO2008136263A1 PCT/JP2008/057411 JP2008057411W WO2008136263A1 WO 2008136263 A1 WO2008136263 A1 WO 2008136263A1 JP 2008057411 W JP2008057411 W JP 2008057411W WO 2008136263 A1 WO2008136263 A1 WO 2008136263A1
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WO
WIPO (PCT)
Prior art keywords
composition
dna ligase
unfreezable
reaction composition
ligase reaction
Prior art date
Application number
PCT/JP2008/057411
Other languages
English (en)
French (fr)
Inventor
Toshihiro Kuroita
Original Assignee
Toyo Boseki Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyo Boseki Kabushiki Kaisha filed Critical Toyo Boseki Kabushiki Kaisha
Publication of WO2008136263A1 publication Critical patent/WO2008136263A1/ja

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

【課題】本発明の目的は、添加剤を検討し、－３０°C保存で少なくとも一週間凍結しないリガーゼ組成物、および使用方法を提供することである。また、それらを含有する試薬・キットおよび製造法を提供することである。 【解決手段】－３０°C保存で少なくとも１週間凍結しない、ＤＮＡリガーゼ反応組成物であって、好ましくはベタイン及びグリセロールを含有する組成物。
PCT/JP2008/057411 2007-04-27 2008-04-16 凍結しないｄｎａリガーゼ反応組成物 WO2008136263A1 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-118426 2007-04-27
JP2007118426A JP2008271834A (ja) 2007-04-27 2007-04-27 凍結しないｄｎａリガーゼ反応組成物

Publications (1)

Publication Number Publication Date
WO2008136263A1 true WO2008136263A1 (ja) 2008-11-13

Family

ID=39943380

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/057411 WO2008136263A1 (ja) 2007-04-27 2008-04-16 凍結しないｄｎａリガーゼ反応組成物

Country Status (2)

Country Link
JP (1) JP2008271834A (ja)
WO (1) WO2008136263A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014517688A (ja) * 2011-05-06 2014-07-24 ニユー・イングランド・バイオレイブス・インコーポレイテツド ライゲーションの促進

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01132362A (ja) * 1987-11-19 1989-05-24 Taiyo Fishery Co Ltd 非凍結保存法
JPH0423987A (ja) * 1990-05-16 1992-01-28 Toyobo Co Ltd 安定化された制限酵素ＳｆｉＩ組成物
JPH07194378A (ja) * 1993-12-28 1995-08-01 Fuji Seito Kk トレハロースによる酵素の安定化法
JP2002034558A (ja) * 2000-07-21 2002-02-05 Nof Corp 安定化された蛋白質分解酵素およびその用途
JP2006025637A (ja) * 2004-07-13 2006-02-02 Toyobo Co Ltd Ｄｎａリガーゼによるライゲーション反応の促進方法およびｄｎａリガーゼ組成物

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01132362A (ja) * 1987-11-19 1989-05-24 Taiyo Fishery Co Ltd 非凍結保存法
JPH0423987A (ja) * 1990-05-16 1992-01-28 Toyobo Co Ltd 安定化された制限酵素ＳｆｉＩ組成物
JPH07194378A (ja) * 1993-12-28 1995-08-01 Fuji Seito Kk トレハロースによる酵素の安定化法
JP2002034558A (ja) * 2000-07-21 2002-02-05 Nof Corp 安定化された蛋白質分解酵素およびその用途
JP2006025637A (ja) * 2004-07-13 2006-02-02 Toyobo Co Ltd Ｄｎａリガーゼによるライゲーション反応の促進方法およびｄｎａリガーゼ組成物

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"TaKaRa Bio Sogo Catalog 2002-2003", 2002, pages A-63 - A-64 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014517688A (ja) * 2011-05-06 2014-07-24 ニユー・イングランド・バイオレイブス・インコーポレイテツド ライゲーションの促進

Also Published As

Publication number Publication date
JP2008271834A (ja) 2008-11-13

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