WO2008110914A3 - Procédés de production d'anticorps scfv actifs et bibliothèques de ceux-ci - Google Patents
Procédés de production d'anticorps scfv actifs et bibliothèques de ceux-ci Download PDFInfo
- Publication number
- WO2008110914A3 WO2008110914A3 PCT/IB2008/000628 IB2008000628W WO2008110914A3 WO 2008110914 A3 WO2008110914 A3 WO 2008110914A3 IB 2008000628 W IB2008000628 W IB 2008000628W WO 2008110914 A3 WO2008110914 A3 WO 2008110914A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- libraries
- methods
- therefor
- scfv antibodies
- producing active
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/567—Framework region [FR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/80—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Computational Biology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Neurology (AREA)
- Oncology (AREA)
- Neurosurgery (AREA)
- Virology (AREA)
- Communicable Diseases (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP08719320.7A EP2134841B9 (fr) | 2007-03-15 | 2008-03-17 | Procédés de production d'anticorps scfv actifs et bibliothèques de ceux-ci |
CA2681170A CA2681170C (fr) | 2007-03-15 | 2008-03-17 | Procedes de production d'anticorps scfv actifs et bibliotheques de ceux-ci |
US12/531,182 US8841238B2 (en) | 2007-03-15 | 2008-03-17 | Methods for producing active scFv antibodies and libraries therefor |
JP2009553231A JP5385796B2 (ja) | 2007-03-15 | 2008-03-17 | 活性なscFv抗体及びそのライブラリーを生産する方法 |
ES08719320T ES2384006T3 (es) | 2007-03-15 | 2008-03-17 | Métodos para producir anticuerpos scFv activos y bibliotecas para los mismos |
AT08719320T ATE546524T1 (de) | 2007-03-15 | 2008-03-17 | Verfahren zur herstellung aktiver scfv-antikörper und bibliotheken dafür |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US89494707P | 2007-03-15 | 2007-03-15 | |
US60/894,947 | 2007-03-15 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2008110914A2 WO2008110914A2 (fr) | 2008-09-18 |
WO2008110914A3 true WO2008110914A3 (fr) | 2009-02-26 |
Family
ID=39760160
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2008/000628 WO2008110914A2 (fr) | 2007-03-15 | 2008-03-17 | Procédés de production d'anticorps scfv actifs et bibliothèques de ceux-ci |
Country Status (7)
Country | Link |
---|---|
US (2) | US8841238B2 (fr) |
EP (1) | EP2134841B9 (fr) |
JP (1) | JP5385796B2 (fr) |
AT (1) | ATE546524T1 (fr) |
CA (1) | CA2681170C (fr) |
ES (1) | ES2384006T3 (fr) |
WO (1) | WO2008110914A2 (fr) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MX2011012299A (es) * | 2009-05-20 | 2012-03-29 | Novimmune Sa | Librerias de polipeptidos sinteticos y metodos para generar variantes de polipeptido naturalmente diversificadas. |
EP2548030B1 (fr) * | 2010-03-18 | 2015-05-27 | Cornell University | Préparation d'anticorps correctement pliés utilisant la présentation sur la membrane intérieure d'intermédiaires de translocation twin-arginine |
WO2011130305A2 (fr) * | 2010-04-12 | 2011-10-20 | Sorrento Therapeutics Inc. | Procédé pour afficher les anticorps |
EP2752487A1 (fr) | 2013-01-03 | 2014-07-09 | Sanofi | Criblage phénotypique intracellulaire |
WO2015133882A1 (fr) * | 2014-03-07 | 2015-09-11 | 사회복지법인 삼성생명공익재단 | Banque d'anticorps scfv, son procédé de préparation et procédé de criblage d'anticorps scfv l'utilisant |
EP3724378A4 (fr) | 2017-12-11 | 2021-12-08 | Abalone Bio, Inc. | Présentation de protéines sur levure dans l'espace périplasmique |
WO2024038095A1 (fr) | 2022-08-16 | 2024-02-22 | Iome Bio | Nouveaux anticorps anti-rgmb |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999045959A1 (fr) * | 1998-03-13 | 1999-09-16 | Dana-Farber Cancer Institute, Inc. | Anticorps humanises et utilisations correspondantes |
WO2000054057A1 (fr) * | 1999-03-10 | 2000-09-14 | Medical Research Council | Selection d'immunoglobulines intracellulaires |
WO2001048017A1 (fr) * | 1999-12-28 | 2001-07-05 | Esbatech Ag | Intracorps a charpente definie stable dans un environnement reducteur et leurs applications |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5858657A (en) | 1992-05-15 | 1999-01-12 | Medical Research Council | Methods for producing members of specific binding pairs |
ES2136092T3 (es) * | 1991-09-23 | 1999-11-16 | Medical Res Council | Procedimientos para la produccion de anticuerpos humanizados. |
US6058338A (en) | 1999-02-12 | 2000-05-02 | Qualcomm Incorporated | Method and apparatus for efficient GPS assistance in a communication system |
US20040096826A1 (en) | 2002-01-30 | 2004-05-20 | Evans Glen A. | Methods for creating recombination products between nucleotide sequences |
US7332580B2 (en) * | 2002-04-05 | 2008-02-19 | The Regents Of The University Of California | Bispecific single chain Fv antibody molecules and methods of use thereof |
-
2008
- 2008-03-17 US US12/531,182 patent/US8841238B2/en active Active
- 2008-03-17 CA CA2681170A patent/CA2681170C/fr active Active
- 2008-03-17 US US12/049,683 patent/US20090143247A1/en not_active Abandoned
- 2008-03-17 EP EP08719320.7A patent/EP2134841B9/fr active Active
- 2008-03-17 JP JP2009553231A patent/JP5385796B2/ja active Active
- 2008-03-17 ES ES08719320T patent/ES2384006T3/es active Active
- 2008-03-17 AT AT08719320T patent/ATE546524T1/de active
- 2008-03-17 WO PCT/IB2008/000628 patent/WO2008110914A2/fr active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999045959A1 (fr) * | 1998-03-13 | 1999-09-16 | Dana-Farber Cancer Institute, Inc. | Anticorps humanises et utilisations correspondantes |
WO2000054057A1 (fr) * | 1999-03-10 | 2000-09-14 | Medical Research Council | Selection d'immunoglobulines intracellulaires |
WO2001048017A1 (fr) * | 1999-12-28 | 2001-07-05 | Esbatech Ag | Intracorps a charpente definie stable dans un environnement reducteur et leurs applications |
Non-Patent Citations (7)
Title |
---|
AUF DER MAUR ADRIAN ET AL: "Antigen-independent selection of intracellular stable antibody frameworks.", METHODS (SAN DIEGO, CALIF.) OCT 2004, vol. 34, no. 2, October 2004 (2004-10-01), pages 215 - 224, XP004526807, ISSN: 1046-2023 * |
DER MAUR ADRIAN AUF ET AL: "Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework.", THE JOURNAL OF BIOLOGICAL CHEMISTRY 22 NOV 2002, vol. 277, no. 47, 22 November 2002 (2002-11-22), pages 45075 - 45085, XP002284890, ISSN: 0021-9258 * |
JUNG S ET AL: "IMPROVING IN VIVO FOLDING AND STABILITY OF A SINGLE-CHAIN FV ANTIBODY FRAGMENT BY LOOP GRAFTING", PROTEIN ENGINEERING, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 10, no. 8, 1 January 1997 (1997-01-01), pages 959 - 966, XP000971779, ISSN: 0269-2139 * |
LADEN JEAN-CHARLES ET AL: "Expression and folding of an antibody fragment selected in vivo for high expression levels in Escherichia coli cytoplasm.", RESEARCH IN MICROBIOLOGY SEP 2002, vol. 153, no. 7, September 2002 (2002-09-01), pages 469 - 474, XP002509241, ISSN: 0923-2508 * |
MARTINEAU P ET AL: "Expression of an antibody fragment at high levels in the bacterial cytoplasm", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 280, no. 1, 3 July 1998 (1998-07-03), pages 117 - 127, XP004453942, ISSN: 0022-2836 * |
MARTINEAU P ET AL: "IN VITRO FOLDING AND THERMODYNAMIC STABILITY OF AN ANTIBODY FRAGMENT SELECTED IN VIVO FOR HIGH EXPRESSION LEVELS IN ESCHERICHIACOLI CYTOPLASM", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 292, 1 October 1999 (1999-10-01), pages 921 - 929, XP004462583, ISSN: 0022-2836 * |
PHILIBERT PASCAL ET AL: "A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm.", BMC BIOTECHNOLOGY 2007, vol. 7, 22 November 2007 (2007-11-22), pages 81, XP002509301, ISSN: 1472-6750 * |
Also Published As
Publication number | Publication date |
---|---|
US20090143247A1 (en) | 2009-06-04 |
EP2134841B9 (fr) | 2014-06-11 |
CA2681170C (fr) | 2017-10-31 |
WO2008110914A2 (fr) | 2008-09-18 |
ATE546524T1 (de) | 2012-03-15 |
EP2134841B1 (fr) | 2012-02-22 |
EP2134841A2 (fr) | 2009-12-23 |
US8841238B2 (en) | 2014-09-23 |
JP5385796B2 (ja) | 2014-01-08 |
JP2010521447A (ja) | 2010-06-24 |
CA2681170A1 (fr) | 2008-09-18 |
ES2384006T3 (es) | 2012-06-28 |
US20100167957A1 (en) | 2010-07-01 |
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