WO2008105432A1 - アンモニア低発生性納豆菌、該納豆菌を用いた納豆の製造方法及び納豆 - Google Patents

アンモニア低発生性納豆菌、該納豆菌を用いた納豆の製造方法及び納豆 Download PDF

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Publication number
WO2008105432A1
WO2008105432A1 PCT/JP2008/053336 JP2008053336W WO2008105432A1 WO 2008105432 A1 WO2008105432 A1 WO 2008105432A1 JP 2008053336 W JP2008053336 W JP 2008053336W WO 2008105432 A1 WO2008105432 A1 WO 2008105432A1
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WO
WIPO (PCT)
Prior art keywords
natto
bacterium
ammonia
less
natto bacterium
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Application number
PCT/JP2008/053336
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English (en)
French (fr)
Inventor
Shigeki Kada
Masahiro Yabusaki
Original Assignee
Mizkan Group Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mizkan Group Corporation filed Critical Mizkan Group Corporation
Priority to JP2009501259A priority Critical patent/JP4657366B2/ja
Publication of WO2008105432A1 publication Critical patent/WO2008105432A1/ja

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00—Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/50—Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Nutrition Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Polymers & Plastics (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Beans For Foods Or Fodder (AREA)

Abstract

【課題】高温条件での納豆製品の流通過程において、急速かつ多量に発生するアンモニアを低減しうる納豆菌を育種、開発し、またその納豆菌を用いて納豆を生産することにより鮮度が保持され、再発酵を起こさずに、アンモニア濃度が少なく、アンモニア臭のしない納豆の生産方法を提供すること。 【解決手段】納豆菌の尿素資化能及びプロリン資化能を低下させた納豆菌、即ち、尿素資化率を１５％以下とし、プロリン資化率を５％以下とした納豆菌、又は、ウレアーゼ遺伝子ならびにグルタミン酸脱水素酵素遺伝子を欠損させてウレアーゼ及びグルタミン酸脱水素酵素の活性を低下ないし欠損させた納豆菌、即ち、ウレアーゼ活性が４０ユニット／ｍｇ蛋白質以下及びグルタミン酸脱水素酵素活性が１４０ユニット／ｍｇ蛋白質以下とした納豆菌を開発し、該納豆菌を用いて、例えば、アンモニア濃度が４０ｐｐｍ未満の納豆を提供することができる。
PCT/JP2008/053336 2007-02-27 2008-02-26 アンモニア低発生性納豆菌、該納豆菌を用いた納豆の製造方法及び納豆 WO2008105432A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2009501259A JP4657366B2 (ja) 2007-02-27 2008-02-26 アンモニア低発生性納豆菌、該納豆菌を用いた納豆の製造方法及び納豆

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-047703 2007-02-27
JP2007047703 2007-02-27

Publications (1)

Publication Number Publication Date
WO2008105432A1 true WO2008105432A1 (ja) 2008-09-04

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/053336 WO2008105432A1 (ja) 2007-02-27 2008-02-26 アンモニア低発生性納豆菌、該納豆菌を用いた納豆の製造方法及び納豆

Country Status (2)

Country Link
JP (1) JP4657366B2 (ja)
WO (1) WO2008105432A1 (ja)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011160779A (ja) * 2010-02-15 2011-08-25 Akita Prefectural Univ 酵母の自然変異株分離法及び変異株酵母
JP4918173B1 (ja) * 2011-09-13 2012-04-18 あづま食品株式会社 新規納豆菌及びこれを用いて製造した納豆
JP2013507912A (ja) * 2009-10-15 2013-03-07 株式会社アモーレパシフィック 発酵茶抽出物を含有する血液循環改善用及び手足冷え性改善用組成物
CN109022326A (zh) * 2018-08-28 2018-12-18 云南大学 一株产尿素细菌及其应用
CN113208057A (zh) * 2021-06-08 2021-08-06 北京燕京中发生物技术有限公司 一种含高活性纳豆激酶的纳豆及其制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06269281A (ja) * 1993-03-22 1994-09-27 Aomori Pref Gov アンモニア発生量およびプロテアーゼ活性の低い納豆菌及びそれを用いた納豆の製造方法
JPH08154616A (ja) * 1994-12-09 1996-06-18 Kikkoman Corp 品質改良納豆および新規変異菌株
JPH08275772A (ja) * 1995-04-06 1996-10-22 Fujitsuko Kk 納豆菌およびそれを用いた納豆の製法並びに納豆
JP2000224982A (ja) * 1999-01-13 2000-08-15 Mitsukan Group Honsha:Kk 短鎖分岐脂肪酸非生産納豆菌
JP2000354493A (ja) * 1999-04-16 2000-12-26 Mitsukan Group Honsha:Kk ムレ臭減感ないし非感納豆

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06269281A (ja) * 1993-03-22 1994-09-27 Aomori Pref Gov アンモニア発生量およびプロテアーゼ活性の低い納豆菌及びそれを用いた納豆の製造方法
JPH08154616A (ja) * 1994-12-09 1996-06-18 Kikkoman Corp 品質改良納豆および新規変異菌株
JPH08275772A (ja) * 1995-04-06 1996-10-22 Fujitsuko Kk 納豆菌およびそれを用いた納豆の製法並びに納豆
JP2000224982A (ja) * 1999-01-13 2000-08-15 Mitsukan Group Honsha:Kk 短鎖分岐脂肪酸非生産納豆菌
JP2000354493A (ja) * 1999-04-16 2000-12-26 Mitsukan Group Honsha:Kk ムレ臭減感ないし非感納豆

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BELITSKY B.R. ET AL.: "Role and regulation of Bacillus subtilis glutamate dehydrogenase genes", J. BACTERIOL., vol. 180, no. 23, December 1998 (1998-12-01), pages 6298 - 6305, XP002385305 *
CRUZ-RAMOS H. ET AL.: "The Bacillus subtilis ureABC operon", J. BACTERIOL., vol. 179, no. 10, 1997, pages 3371 - 3373, XP002652911 *
KOGUCHI K. ET AL.: "Nattokin no Ikushu ni Kansuru Kenkyu (2nd report) Nattokin no Kairyo to Ko Fukakachi Nattokin no Ikushu", REPORT OF INDUSTRIAL RESEARCH ORGANIZATION OF TOCHIGI PREFECTURE, vol. 1994, 1995, pages 170 - 173 *
KOGUCHI K. ET AL.: "Nattokin no Ikushu ni Kansuru Kenkyu (4th report) Nattokin no Kairyo to Ko Fukakachi Nattokin no Ikushu", REPORT OF INDUSTRIAL RESEARCH ORGANIZATION OF TOCHIGI PREFECTURE, vol. 1996, 1997, pages 165 - 168 *
ONOZAKI H. ET AL.: "Nioi no Sukunai Natto no Kaihatsu", JOURNAL OF SUGIYAMA JOGAKUEN UNIVERSITY, no. 23, PART 1, 1992, pages 259 - 272 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013507912A (ja) * 2009-10-15 2013-03-07 株式会社アモーレパシフィック 発酵茶抽出物を含有する血液循環改善用及び手足冷え性改善用組成物
US10080375B2 (en) 2009-10-15 2018-09-25 Amorepacific Corporation Composition for improving blood circulation and alleviating cold hands and feet, containing fermented tea extract
JP2011160779A (ja) * 2010-02-15 2011-08-25 Akita Prefectural Univ 酵母の自然変異株分離法及び変異株酵母
JP4918173B1 (ja) * 2011-09-13 2012-04-18 あづま食品株式会社 新規納豆菌及びこれを用いて製造した納豆
CN109022326A (zh) * 2018-08-28 2018-12-18 云南大学 一株产尿素细菌及其应用
CN113208057A (zh) * 2021-06-08 2021-08-06 北京燕京中发生物技术有限公司 一种含高活性纳豆激酶的纳豆及其制备方法

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Publication number Publication date
JPWO2008105432A1 (ja) 2010-06-03
JP4657366B2 (ja) 2011-03-23

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