WO2008092155A3 - Procédé et système de séquençage génétique efficace par données d'image - Google Patents

Procédé et système de séquençage génétique efficace par données d'image Download PDF

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Publication number
WO2008092155A3
WO2008092155A3 PCT/US2008/052212 US2008052212W WO2008092155A3 WO 2008092155 A3 WO2008092155 A3 WO 2008092155A3 US 2008052212 W US2008052212 W US 2008052212W WO 2008092155 A3 WO2008092155 A3 WO 2008092155A3
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WIPO (PCT)
Prior art keywords
image data
sites
sequencing method
sequencing
genetic sequencing
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PCT/US2008/052212
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English (en)
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WO2008092155A2 (fr
Inventor
David L Heiner
Robert C Kain
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Illumina Inc
David L Heiner
Robert C Kain
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Application filed by Illumina Inc, David L Heiner, Robert C Kain filed Critical Illumina Inc
Priority to EP08728407A priority Critical patent/EP2126766A2/fr
Publication of WO2008092155A2 publication Critical patent/WO2008092155A2/fr
Publication of WO2008092155A3 publication Critical patent/WO2008092155A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

La présente invention concerne une technique permettant d'effectuer le séquençage de polymères tels que l'ADN et l'ARN. Un échantillon contenant de multiples sites à séquencer est soumis de manière cyclique à une fixation de nucléotides, et analysé dans un dispositif d'imagerie. Un masque numérique correspondant aux sites d'intérêt de l'échantillon est généré et les données d'image des sites sont traitées différemment des données d'image ne correspondant pas aux sites d'intérêt. Ces dernières peuvent être éliminées au cours du processus de séquençage. L'utilisation du masque améliore l'efficacité informatique et réduit la mémoire allouée aux données d'image pendant le séquençage.
PCT/US2008/052212 2007-01-26 2008-01-28 Procédé et système de séquençage génétique efficace par données d'image WO2008092155A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP08728407A EP2126766A2 (fr) 2007-01-26 2008-01-28 Procédé et système de séquençage génétique efficace par données d'image

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US89764607P 2007-01-26 2007-01-26
US89764707P 2007-01-26 2007-01-26
US60/897,647 2007-01-26
US60/897,646 2007-01-26

Publications (2)

Publication Number Publication Date
WO2008092155A2 WO2008092155A2 (fr) 2008-07-31
WO2008092155A3 true WO2008092155A3 (fr) 2008-11-13

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2008/052212 WO2008092155A2 (fr) 2007-01-26 2008-01-28 Procédé et système de séquençage génétique efficace par données d'image
PCT/US2008/052198 WO2008092150A1 (fr) 2007-01-26 2008-01-28 Système et procédé de séquençage d'acides nucléiques

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2008/052198 WO2008092150A1 (fr) 2007-01-26 2008-01-28 Système et procédé de séquençage d'acides nucléiques

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US (6) US8725425B2 (fr)
EP (3) EP2126766A2 (fr)
JP (1) JP2010516285A (fr)
CN (1) CN101652780B (fr)
AT (1) ATE521948T1 (fr)
CA (1) CA2676570C (fr)
WO (2) WO2008092155A2 (fr)

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US9797012B2 (en) 2017-10-24
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US8244479B2 (en) 2012-08-14
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