CN105637126B - 使用电方法的分子分析物的数字分析 - Google Patents
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B20/00—Methods specially adapted for identifying library members
- C40B20/04—Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
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Abstract
Description
循环# | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 数字标识 |
靶标1:L-选择素 | a | b | c | d | e | f | G | h | deabfcgh |
靶标2:白蛋白 | i | j | k | l | m | n | O | p | pnmolijk |
尾区编号 | 前导序列长度 | 终止碱基 | 拖尾序列长度 |
尾区#1 | 100 | C | 800 |
尾区#2 | 200 | C | 700 |
尾区#3 | 300 | C | 600 |
尾区#4 | 400 | C | 500 |
尾区#5 | 500 | C | 400 |
尾区#6 | 600 | C | 300 |
尾区#7 | 700 | C | 200 |
尾区#8 | 800 | C | 100 |
流序列编号 | 流核苷酸 | 描述 |
序列#1 | T | 拖尾序列长度ID |
序列#2 | G | 除去终止碱基‘C’ |
序列#3 | T | 前导序列长度ID |
序列#4 | C | 除去终止碱基‘G’ |
序列#5 | T | 前导序列长度ID |
序列#6 | A | 除去终止碱基‘T’ |
序列#7 | T | 前导序列长度ID |
流序列编号 | 流核苷酸 | 描述 |
序列#1 | T | 拖尾序列长度ID |
序列#2 | G | 除去终止2碱基‘C’ |
序列#3 | T | 中间序列长度ID |
序列#4 | C | 除去终止2碱基‘G’ |
序列#5 | T | 中间序列长度ID |
序列#6 | A | 除去终止2碱基‘T’ |
序列#7 | T | 中间序列长度ID |
序列#8 | G | 除去终止1碱基‘C’ |
序列#9 | T | 前导序列长度ID |
序列#10 | C | 除去终止1碱基‘G’ |
序列#11 | T | 前导序列长度ID |
序列#12 | A | 除去终止1碱基‘T’ |
序列#13 | T | 前导序列长度ID |
流序列编号 | 流核苷酸 | 描述 |
序列#1 | T | 拖尾序列长度ID,所有标签 |
序列#2 | G | 除去终止2碱基‘C’ |
序列#3 | T | 中间序列长度ID,‘C’ |
序列#4 | C | 除去终止2碱基‘G’ |
序列#5 | T | 中间序列长度ID,‘G’ |
序列#6 | G | 除去终止1碱基‘C’ |
序列#7 | T | 前导序列长度ID,‘C’ |
序列#8 | C | 除去终止1碱基‘G’ |
序列#9 | T | 前导序列长度ID,‘G’ |
1C1C8 | 1C2C7 | 1C3C6 | 1C4C5 | 1C5C4 | 1C6C3 | 1C7C2 | 1C8C1 |
2C1C7 | 2C2C6 | 2C3C5 | 2C4C4 | 2C5C3 | 2C6C2 | 2C7C1 | 3C1C6 |
3C2C5 | 3C3C4 | 3C4C3 | 3C5C2 | 3C6C1 | 4C1C5 | 4C2C4 | 4C3C3 |
4C4C2 | 4C5C1 | 5C1C4 | 5C2C3 | 5C3C2 | 5C4C1 | 6C1C3 | 6C2C2 |
6C3C1 | 7C1C2 | 7C2C1 | 8C1G1 | 1C1G8 | 1C2G7 | 1C3G6 | 1C4G5 |
1C5G4 | 1C6G3 | 1C7G2 | 1C8G1 | 2C1G7 | 2C2G6 | 2C3G5 | 2C4G4 |
2C5G3 | 2C6G2 | 2C7G1 | 3C1G6 | 3C2G5 | 3C3G4 | 3C4G3 | 3C5G2 |
3C6G1 | 4C1G5 | 4C2G4 | 4C3G3 | 4C4G2 | 4C5G1 | 5C1G4 | 5C2G3 |
5C3G2 | 5C4G1 | 6C1G3 | 6C2G2 | 6C3G1 | 7C1G2 | 7C2G1 | 8C1G1 |
1C1T8 | 1C2T7 | 1C3T6 | 1C4T5 | 1C5T4 | 1C6T3 | 1C7T2 | 1C8T1 |
2C1T7 | 2C2T6 | 2C3T5 | 2C4T4 | 2C5T3 | 2C6T2 | 2C7T1 | 3C1T6 |
3C2T5 | 3C3T4 | 3C4T3 | 3C5T2 | 3C6T1 | 4C1T5 | 4C2T4 | 4C3T3 |
4C4T2 | 4C5T1 | 5C1T4 | 5C2T3 | 5C3T2 | 5C4T1 | 6C1T3 | 6C2T2 |
6C3T1 | 7C1T2 | 7C2T1 | 8C1T1 | 1G1C8 | 1G2C7 | 1G3C6 | 1G4C5 |
1G5C4 | 1G6C3 | 1G7C2 | 1G8C1 | 2G1C7 | 2G2C6 | 2G3C5 | 2G4C4 |
2G5C3 | 2G6C2 | 2G7C1 | 3G1C6 | 3G2C5 | 3G3C4 | 3G4C3 | 3G5C2 |
3G6C1 | 4G1C5 | 4G2C4 | 4G3C3 | 4G4C2 | 4G5C1 | 5G1C4 | 5G2C3 |
5G3C2 | 5G4C1 | 6G1C3 | 6G2C2 | 6G3C1 | 7G1C2 | 7G2C1 | 8G1G1 |
1G1G8 | 1G2G7 | 1G3G6 | 1G4G5 | 1G5G4 | 1G6G3 | 1G7G2 | 1G8G1 |
2G1G7 | 2G2G6 | 2G3G5 | 2G4G4 | 2G5G3 | 2G6G2 | 2G7G1 | 3G1G6 |
3G2G5 | 3G3G4 | 3G4G3 | 3G5G2 | 3G6G1 | 4G1G5 | 4G2G4 | 4G3G3 |
4G4G2 | 4G5G1 | 5G1G4 | 5G2G3 | 5G3G2 | 5G4G1 | 6G1G3 | 6G2G2 |
6G3G1 | 7G1G2 | 7G2G1 | 8G1G1 | 1G1T8 | 1G2T7 | 1G3T6 | 1G4T5 |
1G5T4 | 1G6T3 | 1G7T2 | 1G8T1 | 2G1T7 | 2G2T6 | 2G3T5 | 2G4T4 |
2G5T3 | 2G6T2 | 2G7T1 | 3G1T6 | 3G2T5 | 3G3T4 | 3G4T3 | 3G5T2 |
3G6T1 | 4G1T5 | 4G2T4 | 4G3T3 | 4G4T2 | 4G5T1 | 5G1T4 | 5G2T3 |
5G3T2 | 5G4T1 | 6G1T3 | 6G2T2 | 6G3T1 | 7G1T2 | 7G2T1 | 8G1T1 |
1T1C8 | 1T2C7 | 1T3C6 | 1T4C5 | 1T5C4 | 1T6C3 | 1T7C2 | 1T8C1 |
2T1C7 | 2T2C6 | 2T3C5 | 2T4C4 | 2T5C3 | 2T6C2 | 2T7C1 | 3T1C6 |
3T2C5 | 3T3C4 | 3T4C3 | 3T5C2 | 3T6C1 | 4T1C5 | 4T2C4 | 4T3C3 |
4T4C2 | 4T5C1 | 5T1C4 | 5T2C3 | 5T3C2 | 5T4C1 | 6T1C3 | 6T2C2 |
6T3C1 | 7T1C2 | 7T2C1 | 8T1G1 | 1T1G8 | 1T2G7 | 1T3G6 | 1T4G5 |
1T5G4 | 1T6G3 | 1T7G2 | 1T8G1 | 2T1G7 | 2T2G6 | 2T3G5 | 2T4G4 |
2T5G3 | 2T6G2 | 2T7G1 | 3T1G6 | 3T2G5 | 3T3G4 | 3T4G3 | 3T5G2 |
3T6G1 | 4T1G5 | 4T2G4 | 4T3G3 | 4T4G2 | 4T5G1 | 5T1G4 | 5T2G3 |
5T3G2 | 5T4G1 | 6T1G3 | 6T2G2 | 6T3G1 | 7T1G2 | 7T2G1 | 8T1G1 |
1T1T8 | 1T2T7 | 1T3T6 | 1T4T5 | 1T5T4 | 1T6T3 | 1T7T2 | 1T8T1 |
2T1T7 | 2T2T6 | 2T3T5 | 2T4T4 | 2T5T3 | 2T6T2 | 2T7T1 | 3T1T6 |
3T2T5 | 3T3T4 | 3T4T3 | 3T5T2 | 3T6T1 | 4T1T5 | 4T2T4 | 4T3T3 |
4T4T2 | 4T5T1 | 5T1T4 | 5T2T3 | 5T3T2 | 5T4T1 | 6T1T3 | 6T2T2 |
6T3T1 | 7T1T2 | 7T2T1 | 8T1T1 |
流序列编号 | 流核苷酸 | 描述 |
序列#1 | T | 拖尾序列长度ID,所有标签 |
序列#2 | G | 除去终止2碱基‘C’ |
序列#3 | T | 中间序列长度ID,‘C’ |
序列#4 | C | 除去终止2碱基‘G’ |
序列#5 | T | 中间序列长度ID,‘G’ |
序列#6 | A | 除去终止2碱基‘T’ |
序列#7 | T | 中间序列长度ID,‘T’ |
序列#8 | G | 除去终止1碱基‘C’ |
序列#9 | T | 前导序列长度ID,‘C’ |
序列#10 | C | 除去终止1碱基‘G’ |
序列#11 | T | 前导序列长度ID,‘G’ |
序列#12 | A | 除去终止1碱基‘T’ |
序列#13 | T | 前导序列长度ID,‘T’ |
#水平 | #终止 | #终止类型 | #探针 | #每个循环的位 |
3 | 1 | 1 | 2 | 1.00 |
4 | 1 | 1 | 3 | 1.58 |
5 | 1 | 1 | 4 | 2.00 |
6 | 1 | 1 | 5 | 2.32 |
7 | 1 | 1 | 6 | 2.58 |
8 | 1 | 1 | 7 | 2.81 |
9 | 1 | 1 | 8 | 3.00 Ex#1 |
10 | 1 | 1 | 9 | 3.17 |
3 | 1 | 2 | 4 | 2.00 |
4 | 1 | 2 | 6 | 2.58 |
5 | 1 | 2 | 8 | 3.00 |
6 | 1 | 2 | 10 | 3.32 |
7 | 1 | 2 | 12 | 3.58 |
8 | 1 | 2 | 14 | 3.81 |
9 | 1 | 2 | 16 | 4.00 |
10 | 1 | 2 | 18 | 4.17 |
3 | 1 | 3 | 6 | 2.58 |
4 | 1 | 3 | 9 | 3.17 |
5 | 1 | 3 | 12 | 3.58 |
6 | 1 | 3 | 15 | 3.91 |
7 | 1 | 3 | 18 | 4.17 Ex#2 |
8 | 1 | 3 | 21 | 4.39 |
9 | 1 | 3 | 24 | 4.58 |
10 | 1 | 3 | 27 | 4.75 |
#水平 | #终止 | #终止类型 | #探针 | #每个循环的位 |
3 | 2 | 1 | 1 | 0.00 |
4 | 2 | 1 | 3 | 1.58 |
5 | 2 | 1 | 6 | 2.58 |
6 | 2 | 1 | 10 | 3.32 |
7 | 2 | 1 | 15 | 3.91 |
8 | 2 | 1 | 21 | 4.39 |
9 | 2 | 1 | 28 | 4.81 |
10 | 2 | 1 | 36 | 5.17 |
3 | 2 | 2 | 4 | 2.00 |
4 | 2 | 2 | 12 | 3.58 |
5 | 2 | 2 | 24 | 4.58 |
6 | 2 | 2 | 40 | 5.32 |
7 | 2 | 2 | 60 | 5.91 |
8 | 2 | 2 | 84 | 6.39 Ex#4 |
9 | 2 | 2 | 112 | 6.81 |
10 | 2 | 2 | 144 | 7.17 |
3 | 2 | 3 | 9 | 3.17 |
4 | 2 | 3 | 27 | 4.75 Ex#3 |
5 | 2 | 3 | 54 | 5.75 |
6 | 2 | 3 | 90 | 6.49 |
7 | 2 | 3 | 135 | 7.08 |
8 | 2 | 3 | 189 | 7.56 |
9 | 2 | 3 | 252 | 7.98 |
10 | 2 | 3 | 324 | 8.34 Ex#5 |
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