WO2008090759A1 - マイクロ総合分析システム - Google Patents

マイクロ総合分析システム Download PDF

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Publication number
WO2008090759A1
WO2008090759A1 PCT/JP2008/050116 JP2008050116W WO2008090759A1 WO 2008090759 A1 WO2008090759 A1 WO 2008090759A1 JP 2008050116 W JP2008050116 W JP 2008050116W WO 2008090759 A1 WO2008090759 A1 WO 2008090759A1
Authority
WO
WIPO (PCT)
Prior art keywords
microchip
fluorescence
general
amount
measuring
Prior art date
Application number
PCT/JP2008/050116
Other languages
English (en)
French (fr)
Inventor
Tsuneo Sawazumi
Akihisa Nakajima
Original Assignee
Konica Minolta Medical & Graphic, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Konica Minolta Medical & Graphic, Inc. filed Critical Konica Minolta Medical & Graphic, Inc.
Priority to JP2008555010A priority Critical patent/JPWO2008090759A1/ja
Publication of WO2008090759A1 publication Critical patent/WO2008090759A1/ja

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N2021/7769—Measurement method of reaction-produced change in sensor
    • G01N2021/7786—Fluorescence

Abstract

　蛍光色素で標識されたプローブと標的分子の反応において反応前後での蛍光強度の変化分を正確に測定し、精度良い検出を行うことのできるマイクロ総合分析システムを提供することである。更に、マイクロチップの蛍光検出に適した、特にサイクリングプローブ法を用いたバイオ検査用マイクロチップの蛍光検出に適用したマイクロ総合分析システムを提供することを目的する。 そのためには、マイクロチップ内の化学反応の進行に応じて発せられる蛍光の発光量に基づいてマイクロチップ内の測定部の標的分子の存在や量を測定するマイクロ総合分析システムであって、当該化学反応前後に蛍光発光量を測定し、かつ当該蛍光発光量を測定する際に、マイクロチップ内の試薬及びマイクロチップ内の測定部位を含む反応系の温度を、当該化学反応前後において、同一となるように制御することを特徴とするマイクロ総合分析システムとする。
PCT/JP2008/050116 2007-01-26 2008-01-09 マイクロ総合分析システム WO2008090759A1 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008555010A JPWO2008090759A1 (ja) 2007-01-26 2008-01-09 マイクロ総合分析システム

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007016154 2007-01-26
JP2007-016154 2007-01-26

Publications (1)

Publication Number Publication Date
WO2008090759A1 true WO2008090759A1 (ja) 2008-07-31

Family

ID=39644334

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050116 WO2008090759A1 (ja) 2007-01-26 2008-01-09 マイクロ総合分析システム

Country Status (2)

Country Link
JP (1) JPWO2008090759A1 (ja)
WO (1) WO2008090759A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013210308A (ja) * 2012-03-30 2013-10-10 Sony Corp マイクロチップローディング装置、マイクロチップ型フローサイトメータ及びマイクロチップローディング方法
JP2016040526A (ja) * 2014-08-12 2016-03-24 横河電機株式会社 プロセスガスクロマトグラフ、および昇温槽の温度調整方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0870876A (ja) * 1994-09-01 1996-03-19 F Hoffmann La Roche Ag 発光性標識で標識されたオリゴヌクレオチドの分解検出方法
JP2002355084A (ja) * 2001-03-27 2002-12-10 National Institute Of Advanced Industrial & Technology 新規核酸プローブおよびそれを用いる新規核酸測定方法
JP2005323519A (ja) * 2004-05-13 2005-11-24 Konica Minolta Sensing Inc マイクロ流体デバイス並びに試液の試験方法および試験システム

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0870876A (ja) * 1994-09-01 1996-03-19 F Hoffmann La Roche Ag 発光性標識で標識されたオリゴヌクレオチドの分解検出方法
JP2002355084A (ja) * 2001-03-27 2002-12-10 National Institute Of Advanced Industrial & Technology 新規核酸プローブおよびそれを用いる新規核酸測定方法
JP2005323519A (ja) * 2004-05-13 2005-11-24 Konica Minolta Sensing Inc マイクロ流体デバイス並びに試液の試験方法および試験システム

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013210308A (ja) * 2012-03-30 2013-10-10 Sony Corp マイクロチップローディング装置、マイクロチップ型フローサイトメータ及びマイクロチップローディング方法
JP2016040526A (ja) * 2014-08-12 2016-03-24 横河電機株式会社 プロセスガスクロマトグラフ、および昇温槽の温度調整方法

Also Published As

Publication number Publication date
JPWO2008090759A1 (ja) 2010-05-20

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