WO2008084857A1 - 三次元細胞培養担体およびそれを用いた細胞培養方法 - Google Patents

三次元細胞培養担体およびそれを用いた細胞培養方法 Download PDF

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Publication number
WO2008084857A1
WO2008084857A1 PCT/JP2008/050286 JP2008050286W WO2008084857A1 WO 2008084857 A1 WO2008084857 A1 WO 2008084857A1 JP 2008050286 W JP2008050286 W JP 2008050286W WO 2008084857 A1 WO2008084857 A1 WO 2008084857A1
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WIPO (PCT)
Prior art keywords
cell culture
fibers
same
dimensional
fibrous structure
Prior art date
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PCT/JP2008/050286
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English (en)
French (fr)
Inventor
Hiroshi Kambayashi
Masanobu Tsuda
Noriaki Sato
Keizaburo Miki
Nanako Mitani
Original Assignee
Nippon Sheet Glass Company, Limited
Bios Research Institute Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Sheet Glass Company, Limited, Bios Research Institute Inc. filed Critical Nippon Sheet Glass Company, Limited
Priority to JP2008553126A priority Critical patent/JP4851543B2/ja
Priority to US12/523,003 priority patent/US20100041146A1/en
Publication of WO2008084857A1 publication Critical patent/WO2008084857A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/10—Mineral substrates
    • C12N2533/12—Glass

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　本発明の三次元細胞培養担体は、細胞を培養するための三次元空間を備えた繊維構造体を含んでいる。繊維構造体は、互いに絡まり合った複数の繊維によって形成されている。繊維構造体に用いられる繊維は、厚さ３ｍｍに成形したときの可視光透過率が４０％以上（好ましくは５０％以上）である材料によって形成されている。このような繊維の一例として、例えばガラス繊維が挙げられる。また、この繊維のアスペクト比は１以上であり、繊維径は１００μｍ以上７００μｍ以下である。
PCT/JP2008/050286 2007-01-12 2008-01-11 三次元細胞培養担体およびそれを用いた細胞培養方法 WO2008084857A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2008553126A JP4851543B2 (ja) 2007-01-12 2008-01-11 三次元細胞培養担体およびそれを用いた細胞培養方法
US12/523,003 US20100041146A1 (en) 2007-01-12 2008-01-11 Three-dimensional cell culture carrier and method for cell culture using the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007004691 2007-01-12
JP2007-004691 2007-01-12

Publications (1)

Publication Number Publication Date
WO2008084857A1 true WO2008084857A1 (ja) 2008-07-17

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ID=39608744

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050286 WO2008084857A1 (ja) 2007-01-12 2008-01-11 三次元細胞培養担体およびそれを用いた細胞培養方法

Country Status (3)

Country Link
US (1) US20100041146A1 (ja)
JP (1) JP4851543B2 (ja)
WO (1) WO2008084857A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014030641A1 (ja) * 2012-08-20 2014-02-27 国立大学法人岡山大学 細胞を培養する担体及び培養細胞を用いたタンパク質又はペプチドの生産方法
KR20170093983A (ko) 2015-01-26 2017-08-16 우베 고산 가부시키가이샤 폴리이미드 다공질막을 이용하는 세포의 대량 배양 방법, 장치 및 키트
WO2021029409A1 (ja) 2019-08-09 2021-02-18 宇部興産株式会社 小片多孔膜を適用した細胞培養法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008179756A (ja) * 2006-12-28 2008-08-07 Showa Denko Kk 発光素子封止用樹脂組成物およびランプ
US20130281355A1 (en) * 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
USD802157S1 (en) * 2014-03-02 2017-11-07 Pluristem Ltd. Carrier for cell culture
WO2017146627A1 (en) 2016-02-26 2017-08-31 Neuronano Ab Method of implantation of cell aggregates and tissue fragments

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002535981A (ja) * 1999-02-04 2002-10-29 テクニオン リサーチ アンド デブェロップメント ファウンデーション リミテド 造血幹細胞および／または前駆細胞を維持および増加するための方法および装置
JP2003509021A (ja) * 1999-09-07 2003-03-11 ギルテック・リミテッド 細胞増殖基質

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002535981A (ja) * 1999-02-04 2002-10-29 テクニオン リサーチ アンド デブェロップメント ファウンデーション リミテド 造血幹細胞および／または前駆細胞を維持および増加するための方法および装置
JP2003509021A (ja) * 1999-09-07 2003-03-11 ギルテック・リミテッド 細胞増殖基質

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MIKI K. ET AL.: "Glass Sen'i Shoketsutai o Mochiita Atarashii 3 Jigen Saibo Baiyo Kizai", THE JOURNAL OF EXPERIMENTAL & APPLIED CELL CULTURE RESEARCH, vol. 26, 31 March 2007 (2007-03-31), pages 69 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014030641A1 (ja) * 2012-08-20 2014-02-27 国立大学法人岡山大学 細胞を培養する担体及び培養細胞を用いたタンパク質又はペプチドの生産方法
JPWO2014030641A1 (ja) * 2012-08-20 2016-07-28 国立大学法人 岡山大学 細胞を培養する担体及び培養細胞を用いたタンパク質又はペプチドの生産方法
KR20170093983A (ko) 2015-01-26 2017-08-16 우베 고산 가부시키가이샤 폴리이미드 다공질막을 이용하는 세포의 대량 배양 방법, 장치 및 키트
EP3406710A1 (en) 2015-01-26 2018-11-28 UBE Industries, Ltd. Method, device and kit for mass cultivation of cells using polyimide porous membrane
US10479974B2 (en) 2015-01-26 2019-11-19 Ube Industries, Ltd. Method, device and kit for mass cultivation of cells using polyimide porous membrane
US10696943B2 (en) 2015-01-26 2020-06-30 Ube Industries, Ltd. Method, device and kit for mass cultivation of cells using polyimide porous membrane
WO2021029409A1 (ja) 2019-08-09 2021-02-18 宇部興産株式会社 小片多孔膜を適用した細胞培養法

Also Published As

Publication number Publication date
JPWO2008084857A1 (ja) 2010-05-06
JP4851543B2 (ja) 2012-01-11
US20100041146A1 (en) 2010-02-18

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