WO2008013205A1 - Appareil de traitement d'intégration de type à montage de puce, contenant en forme de puce et procédé de traitement d'intégration de type à montage de puce - Google Patents

Appareil de traitement d'intégration de type à montage de puce, contenant en forme de puce et procédé de traitement d'intégration de type à montage de puce Download PDF

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Publication number
WO2008013205A1
WO2008013205A1 PCT/JP2007/064596 JP2007064596W WO2008013205A1 WO 2008013205 A1 WO2008013205 A1 WO 2008013205A1 JP 2007064596 W JP2007064596 W JP 2007064596W WO 2008013205 A1 WO2008013205 A1 WO 2008013205A1
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WO
WIPO (PCT)
Prior art keywords
nozzle
chip
outer peripheral
wall surface
container
Prior art date
Application number
PCT/JP2007/064596
Other languages
English (en)
Japanese (ja)
Inventor
Hideji Tajima
Tsutomu Asano
Original Assignee
Universal Bio Research Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universal Bio Research Co., Ltd. filed Critical Universal Bio Research Co., Ltd.
Priority to JP2008526792A priority Critical patent/JP4944888B2/ja
Publication of WO2008013205A1 publication Critical patent/WO2008013205A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065—Multiple transfer devices
    • G01N35/1074—Multiple transfer devices arranged in a two-dimensional array

Definitions

  • Chip-mounted integrated processing apparatus chip-shaped container, and chip-mounted integrated processing method
  • the present invention relates to a chip mounting type integrated processing apparatus, a chip-shaped container, and a chip mounting type integrated processing method.
  • a cylinder for driving a plunger is used as a suction / discharge mechanism, but the mechanism such as a plunger is a high-precision machined part such as a syringe, and particularly in a cylinder.
  • the volume change is basically integral with the volume change in the dispensing tip, and needs to be transmitted so that there is no looseness in the joint between the plunger and the drive device of the plunger.
  • Patent Document 1 Japanese Patent No. 3115501
  • Patent Document 2 Japanese Patent No. 3739953
  • Patent Document 3 Japanese Patent No. 3630497
  • Patent Document 4 Japanese Patent No. 3682302
  • a first object of the present invention is to provide a chip mounting type integrated processing apparatus and chip that can smoothly perform simultaneous fitting and simultaneous mounting operations of a plurality of chip containers to a plurality of nozzles. It is to provide a container and a chip mounting type integrated processing method.
  • the second purpose is to provide high water-tightness and air-tightness, so that the reliability is high, the life of the equipment is long, and the burden of quality control is small! /, Chip-mounted integrated processing equipment, chip-shaped container and chip-mounted It is to provide a formula accumulation processing method.
  • a third object is to provide a chip-mounted integrated processing apparatus, a chip-shaped container, and a chip-mounted integrated processing method capable of integrating a plurality of processes and performing a highly efficient and quick process. Is to provide.
  • the first invention includes 1 or two or more nozzles having one or more outer peripheral protrusions projecting outward along two or more closed outer peripheral bands spaced apart from each other in the axial direction of the nozzle, and two or more A nozzle head in which the nozzles are arranged in a predetermined arrangement pattern, a suction / discharge mechanism that sucks and discharges gas through the nozzle, a mounting opening that can be attached to or attached to the nozzle, and a tip.
  • Two or more substantially cylindrical chip containers having a mouth portion through which fluid can be introduced and discharged by suction and discharge of the gas provided, and the chip containers are accommodated in a state that can be mounted on the nozzle in the predetermined arrangement pattern.
  • nozzle head each having a tip-like container accommodating portion that can be accommodated, and a moving means that relatively moves between the nozzle head and the tip-like container accommodating portion.
  • the nozzle is the tip
  • the outer peripheral protrusions provided on the nozzles are in close contact with or in contact with the inner peripheral wall surface of the mounting opening, and the distance from the predetermined reference horizontal plane to at least one of the outer peripheral protrusions is different from each other.
  • This is a chip mounting type integrated processing apparatus composed of a plurality of types of nozzle groups.
  • the “predetermined arrangement pattern” is, for example, a matrix shape, a single row shape, an annular shape, or the like.
  • the “matrix” refers to a structure in which elements, for example, nozzles and the like are arranged in a predetermined number of rows and a number of columns at predetermined row intervals and column intervals along two directions of the column direction and the row direction.
  • the column direction and the row direction are usually orthogonal, but not necessarily limited thereto. It may be crossed.
  • the “nozzle” is a portion where fluid is sucked and discharged, and the fluid includes gas and liquid.
  • the nozzle is a flow path communicating with a “suction / discharge mechanism” such as a cylinder having a plunger. “Suction / discharge” means suction or / and discharge.
  • the "peripheral protrusion” is formed along a belt-like outer peripheral band closed on the outer peripheral side surface of the nozzle so as to surround the nozzle axis, and to protrude outward, radially or normal from the outer peripheral side surface.
  • the outer circumferential band is preferably formed so as to be sandwiched between planes perpendicular to the nozzle axis.
  • the outward force and height of the outer peripheral projection be constant around the nozzle.
  • the band width along the axial direction of the outer edge portion that is in close contact with or in contact with the inner peripheral wall surface is, for example, compared with the width portion of the outer peripheral band on the outer surface, such as a linear or wall thickness of the chip-shaped container. It is preferable for insertion to be formed small.
  • the outer edge of the outer peripheral protrusion may be provided with an o-ring along the outer periphery, or a groove may be provided to embed the o-ring to ensure watertightness and airtightness.
  • “Closely” means that two objects are in contact with each other with substantially no gap, and in this case, the state in which the outer peripheral protrusion is in contact with the inner peripheral wall surface in the entire circumferential length.
  • “Contact” means that two objects come into contact with each other in a state that allows the presence of a gap, and in this case, a part of the entire circumferential length of the outer peripheral protrusion is in contact with the inner peripheral wall surface. Therefore, in the case of “close”, compared to the case of “contact”, the gap between the outer peripheral protrusion and the inner peripheral wall surface is small. Or very small.
  • a “chip-shaped container” is a container having an opening for mounting on a nozzle and a mouth for flowing in and out of a fluid at the tip.
  • the mounting opening and the mouth are preferably provided along the axial direction of the container.
  • the material of the chip-shaped container include resins such as polyethylene, polypropylene, polyester, polystyrene, polyvinyl, and acrylic, and elastic bodies such as rubber.
  • the chip-like container is preferably transparent or translucent. Since it is “substantially cylindrical”, it is substantially cylindrical or substantially rectangular.
  • the size of the chip-like container is, for example, from its mouth
  • the length along the mounting opening or in the axial direction is several centimeters to several tens of centimeters, and the volume is, for example, several microliters to several tens of milliliters depending on the length.
  • the amount of suction and discharge varies depending on the volume, for example, from several microliters to several
  • the chip-shaped container includes, for example, a thick tube portion provided with the mounting opening, a thin tube portion having the mouth portion, and a transition portion formed between the thick tube portion and the thin tube portion. It is not limited to a typical dispensing tip having a tube shape, but may be a tube shape having the same cross-sectional shape along the axial direction as a whole. Moreover, as the shape of the transition portion, for example, a truncated cone shape, a funnel shape, or a step shape is formed.
  • the thick tube and the thin tube are not necessarily limited to a cylindrical shape, and may be a prismatic shape, a polygonal shape, a conical shape, a pyramid shape, or a polygonal pyramid shape.
  • a prepack-type reagent storage container in which a solution containing a reagent, a specimen, or the like is previously stored and the opening is covered with a film is provided.
  • the solution contained in the container can be sucked through the thin tube by piercing the film with the thin tube.
  • Magnetic means capable of applying and removing a magnetic field in the tip-shaped container may be provided in the nozzle head so as to be outside the respective chip-shaped containers.
  • a suspension in which a large number of magnetic substances holding biological compounds such as proteins, peptides, amino acids, DNA, RNA, oligonucleotides, sugar chains, etc. are suspended is placed in the deformable dispensing tip.
  • a magnetic field can be applied to the inside and adsorbed on the inner wall of the chip to separate the magnetic substance, and thus the biological compound.
  • the magnetic force means has, for example, two or more magnets provided so as to be able to come into contact with and separate from two or more of the tip-shaped containers at the same time.
  • the “inner peripheral wall surface of the mounting opening” is formed so as to be in close contact with or in contact with the outer peripheral protrusion. Therefore, for example, a cylindrical surface, a cylindrical surface, a cylindrical surface with a step, a cylindrical surface, a tapered surface, a truncated cone surface, a combination of a cylindrical shape and a truncated cone surface, and the like.
  • the "predetermined reference horizontal plane” is a horizontal plane that serves as a measurement reference set in order to define the distance between the outer peripheral protrusions provided on the nozzle attached to the nozzle head.
  • the surface from which multiple nozzles protrude, the horizontal plane that cuts multiple nozzles It is a horizontal plane set on the top of the page. Usually, it is a plane perpendicular to the axial direction of the nozzle. This is because even if the nozzles have the same shape, the relationship between the mounted chip-like container and the outer peripheral projection differs depending on the state of being attached to the nozzle head.
  • the total length along the axial direction of the nozzles arranged in the nozzle head, or the distance from the nozzle arrangement surface to the tip of the nozzle is preferably the same.
  • the “distance to the outer peripheral protrusion” is, for example, the distance from the predetermined reference water surface to the center of gravity, upper end, lower end, or predetermined position of the outer peripheral protrusion.
  • a nozzle having two or more outer peripheral protrusions projecting outward along two or more closed outer peripheral bands spaced apart from each other in the axial direction of the nozzle one outer periphery between each nozzle It is preferable that not only the protrusions but also other outer peripheral protrusions are formed of nozzle groups having different distances from the predetermined reference horizontal plane to the outer peripheral protrusions that can be in close contact with or in contact with each other. As a result, it is possible to prevent a situation in which a resistance force due to a static frictional force or the like between each outer peripheral protrusion and each inner peripheral wall surface is generated simultaneously for each nozzle.
  • the inner peripheral wall surface of the opening for mounting the chip-like container also has an axis of the container according to each outer peripheral protrusion.
  • Two or more inner peripheral cylindrical wall surfaces spaced apart from each other in the direction (the cross section perpendicular to the axial direction is constant) may be provided, and the gap between them may be a flat surface or a stepped surface.
  • the outer peripheral protrusions of the nozzle and the inner portions of the chip-like containers that are in close contact with or in contact with the outer peripheral protrusions. It is preferable to vary the temporal positional relationship associated with the movement between the peripheral wall surfaces. As a result, it is possible to prevent a situation in which a resistance force due to a static frictional force or the like between the outer peripheral protrusions and the inner peripheral wall surface of the same nozzle is generated at the same time.
  • the arrangement of the nozzles belonging to a plurality of types of nozzle groups is uniform without any spatial deviation in distance or length between at least one outer peripheral protrusion of the nozzle and a predetermined reference horizontal plane. It is preferable to arrange as follows. As a result, when mounting, the entire nozzle head receives the resistance force received by the entire tip of the tip-shaped container and the entire tip of the dispensing tip as much as possible. For example, the distance or length of each nozzle group is set to be longer or shorter for each adjacent column or row, and elements belonging to each column (row) are assigned to the same nozzle group. Arrange the nozzles to which they belong.
  • the nozzle groups are changed and arranged regularly so that the distances or lengths of the adjacent elements in the matrix or in other arrangements become longer or shorter.
  • Each nozzle group has at least one nozzle, and preferably the number of nozzles belonging to each nozzle group is the same or substantially the same. This allows the resistance S to be distributed evenly.
  • the first resistance force received by each of the outer peripheral protrusions from each of the inner peripheral wall surfaces is the static friction coefficient, the outer peripheral protrusion,
  • the size is proportional to the product of the drag in the normal direction between the inner peripheral wall surfaces.
  • the subsequent second resistance force is proportional to the product of the dynamic friction coefficient and the drag in the normal direction between the outer peripheral projection and the inner wall surface, and the dynamic friction coefficient is greater than the static friction coefficient. Generally small! /.
  • the two or more nozzles provided in the nozzle head are formed from a plurality of types of nozzle groups having different distances from the predetermined reference horizontal plane to the outer peripheral protrusion. Then, as the nozzles are simultaneously moved in the insertion direction of the nozzles with respect to the mounting openings of the chip-like containers arranged corresponding to the nozzle arrangement pattern, for example, the distance from the reference horizontal plane set in the nozzle head is the largest.
  • the outer peripheral protrusions of the nozzles belonging to the large first nozzle group first reach a state where they are in close contact with or in contact with the inner peripheral wall surface of the chip-like container. Until this state is reached, the resistance force received by the nozzle from the tip-shaped container is 0 or very small. However, when this state is reached, the outer peripheral projection is caused by a collision with the inner peripheral wall surface or a static frictional force. Suspension or insertion speed is drastically reduced due to resistance. When this resistance force is overcome and the insertion into the nozzle proceeds, the insertion operation continues while receiving a resistance force due to a dynamic friction force smaller than the resistance force.
  • the initial resistance force received by the nozzle is proportional to the product of the number of nozzles, the coefficient of static friction, and the drag in the normal direction acting between the outer peripheral protrusion and the inner peripheral wall surface.
  • the movement of the nozzle head in the insertion direction is continued, it relates to a resistance force having a magnitude using a dynamic friction coefficient instead of the static friction coefficient.
  • the dynamic friction coefficient is smaller than the static friction coefficient
  • the resistance force based on the dynamic friction coefficient is smaller than the resistance force based on the static friction coefficient / J.
  • the outer peripheral protrusion is brought into close contact with or in contact with the inner peripheral wall surface of the tip-shaped container. Buy until. Then, as described above, the number of nozzles belonging to the second nozzle group, the collision between the outer peripheral protrusion and the inner peripheral wall surface, or the coefficient of static friction and the normal line between the outer peripheral protrusion and the inner peripheral wall surface.
  • the resistance force proportional to the product of the directional force is added to the resistance force corresponding to the dynamic friction coefficient of the first nozzle group, and the nozzle head receives the resistance force.
  • liquid storage section group that can store or store various liquids on the stage on which the chip-shaped container storage section is installed.
  • Various liquids include liquids containing various reagents, specimens, chemical substances, or magnetic substances.
  • the two or more outer peripheral protrusions provided on the nozzle are formed such that the outer peripheral length of the outer peripheral protrusion on the front end side is shorter than the outer peripheral length of the outer peripheral protrusion on the rear end side.
  • the chip mounting type integrated processing device is provided with an inner peripheral wall surface in close contact with or in contact with the outer peripheral protrusion corresponding to the outer peripheral protrusion.
  • the two or more outer peripheral protrusions are provided apart from each other, and the inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusions is provided, for example, in the axial direction of the container. It has a peripheral cylindrical wall surface (the cross-sectional shape perpendicular to the axial direction is the same), and a taper surface or a step surface is formed between the inner peripheral cylindrical wall surfaces, or in close contact with two or more outer peripheral protrusions Or there is a tapered surface that comes into contact.
  • the inner peripheral wall surface includes a plurality of inner peripheral cylindrical wall surfaces provided to be spaced apart from each other corresponding to the outer peripheral protrusion, and each inner peripheral cylindrical wall surface is
  • the chip mounting type integrated processing apparatus is provided in close contact with or in contact with the outer peripheral protrusion and provided with a tapered taper surface connected to the inner peripheral cylindrical wall surface on the rear end side of the inner peripheral cylindrical wall surface.
  • a taper surface that expands outward is formed at the rear end of the mounting opening. It is preferable that the edge on the front end side of the tapered surface is continuously connected to the edge on the rear end side of the inner peripheral cylindrical wall surface! [0030] Similarly, with respect to the tapered surface provided between the adjacent inner peripheral cylindrical wall surface on the front end side and the inner peripheral cylindrical wall surface on the rear end side, the front end edge of the tapered surface is the front end side. It is preferable to continuously connect the rear end edge of the inner peripheral cylindrical wall surface and continuously connect the rear end edge of the tapered surface to the front end edge of the inner peripheral cylindrical wall surface on the rear end side.
  • the nozzle is provided with two outer peripheral protrusions spaced apart from each other in the axial direction, and the outer peripheral length of the outer peripheral protrusion on the front end side is shorter than the outer peripheral length of the outer peripheral protrusion on the rear end side.
  • the mounting opening of the chip-shaped container is in close contact with or in contact with the inner peripheral cylindrical wall surface in contact with the outer peripheral protrusion on the front end and the outer peripheral protrusion on the rear end.
  • the rear end side inner peripheral cylindrical wall surface is spaced from the front end side inner peripheral cylindrical wall surface and between the rear end side inner peripheral cylindrical wall surface and the rear end side inner peripheral cylindrical wall surface. It is preferable that a tapered surface be formed on the end side.
  • the moving means belongs to at least the nozzle group after each nozzle of the nozzle head has reached a position where it can be inserted into the mounting opening of the chip-like container.
  • Tip mounting that relatively moves between the nozzle head and the tip-shaped container housing portion so that each nozzle is inserted into the mounting opening based on the nozzle structure and the number of nozzles belonging to each nozzle group This is an integrated processing apparatus.
  • Such movement of the moving means is controlled by the control unit.
  • the control unit further controls the movement between the nozzle head and the chip-shaped container housing unit, further the number or structure of the chip-shaped container, the liquid to be sucked and discharged, the substance contained therein, the amount thereof, It can be controlled based on its storage position, its temperature or its concentration, processing details, or instructions.
  • Substances include various chemical substances including not only biological substances such as nucleic acids, proteins, sugar chains and amino acids but also metals.
  • the fifth aspect of the present invention has a detaching part that detaches the chip-like containers attached to the nozzles all at once, and the detaching part is larger than the maximum outer diameter or maximum width of the horizontal cross section of the nozzle.
  • a desorption plate in which holes or gaps having a diameter or width smaller than the maximum outer diameter or maximum width of the horizontal cross section of the chip-shaped container are formed in accordance with the predetermined arrangement pattern, So
  • the plate surface of the nozzle is provided in parallel to the predetermined reference horizontal plane, and is movable relative to the nozzle along the axial direction of the nozzle so that the axis of the nozzle passes through the hole or the gap. It is the provided chip-shaped container.
  • the “maximum outer diameter or maximum width” is the largest of the outer diameters or widths of the horizontal cross section perpendicular to the axial direction existing in the nozzle or the tip-like container.
  • the “hole” is a closed gap that is formed so as to surround the normal direction of the desorption plate provided for each nozzle, and the nozzle can be inserted into the hole from the normal direction of the desorption plate.
  • the “gap” is a gap provided in the desorption plate and opened in the lateral direction of the desorption plate, and the nozzle can be inserted into the gap from the normal direction of the desorption plate and from the portion opened in the lateral direction.
  • Diameter corresponds to a circumferential shape
  • Width corresponds to something other than a circumferential shape.
  • the nozzle is stopped and the desorption plate is moved downward from the upper side of the chip-like container.
  • the desorption plate is fixed and the nozzle is fixed.
  • the desorption plate may be supported by the nozzle head and provided so as to be movable along the axial direction of the nozzle with respect to the nozzle head.
  • the detachable plate is fixedly provided on the stage provided with the chip-shaped container housing portion, and a gap is formed in the detachable plate, and the nozzle is located on the side of the gap of the detachable plate.
  • the tip container may be detached from the nozzle by raising the nozzle head by the moving means.
  • holes are formed so as to have the same arrangement pattern as the predetermined arrangement pattern, for example, the same number of rows, the same number of columns and the same row spacing, column spacing,
  • An array pattern corresponding to the array pattern for example, a plurality of rows (or rows) of long holes along the column direction (or row direction), and the nozzles of the number of rows or the number of columns) In some cases, it is formed so that it can be inserted.
  • a sixth invention is a substantially cylindrical cylindrical container capable of containing a liquid therein, and protrudes outward along one or two or more closed outer peripheral bands spaced apart from each other in the axial direction.
  • a mounting opening that can be attached to a nozzle having one or more outer peripheral projections, and a mouth that is provided at the tip of the container and allows the fluid to enter and exit by suction and discharge of gas through the nozzle.
  • the opening for mounting is mounted in front of the nozzle by mounting to the nozzle.
  • It is a chip-like container having an inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusion, and a tapered tapered surface that is provided on the rear end side of the inner peripheral wall surface and is connected to the inner peripheral wall surface.
  • the inner peripheral wall surface corresponds to one or more outer peripheral protrusions projecting outward along two or more closed outer peripheral bands spaced apart from each other in the axial direction of the nozzle.
  • the inner peripheral length of the inner peripheral cylindrical wall surface is shorter than the inner peripheral length of the inner peripheral cylindrical wall surface on the rear end side, and a tapered taper surface is formed on the rear end side of the inner peripheral cylindrical wall surface.
  • the outer peripheral length of the outer peripheral protrusion on the front end side is formed shorter than the outer peripheral length of the outer peripheral protrusion on the rear end side, and the inner peripheral cylindrical shape This corresponds to the inner circumference of the wall.
  • the two outer peripheral protrusions provided in the nozzle and spaced apart from each other in the axial direction have an outer peripheral length of the outer peripheral protrusion on the front end side and an outer peripheral protrusion on the rear end side.
  • the opening for mounting of the chip-like container can be in close contact with or in contact with each of the outer peripheral projections, and is provided spaced apart in the axial direction of the container.
  • the inner circumferential length of the inner circumferential cylindrical wall surface of the tip end side is shorter than the inner circumferential length of the rear inner circumferential cylindrical wall surface.
  • a tapered taper surface is formed between the end side and rear end side inner peripheral cylindrical wall surface and the front end side inner peripheral cylindrical wall surface.
  • the tip end side of the nozzle is in close contact with the outer peripheral protruding portion, and the rear end side inner peripheral cylindrical wall surface is a tip-shaped container that comes into contact with the outer end protruding portion on the rear end side.
  • one or more nozzles having one or more outer peripheral protrusions projecting outward along two or more closed outer peripheral bands spaced apart from each other in the axial direction;
  • Nozzle head in which nozzles are arranged in a predetermined arrangement pattern, suction / discharge mechanism that performs suction / discharge of gas through the nozzle, mounting opening that can be attached to the nozzle, and suction / discharge of the gas provided at the tip
  • Two or more substantially chip-shaped containers having a mouth portion through which fluid can be entered and exited, and a chip-shaped container in which the chip-shaped containers are arranged in the predetermined arrangement pattern and can be attached to the nozzles
  • An accommodating portion and a moving means for moving the nozzle head relative to the tip-shaped container accommodating portion, provided on the nozzle head.
  • the outer peripheral protrusions of the nozzles are in close contact with or in contact with the inner peripheral wall surface of the mounting opening by mounting the tip-shaped container, and the outer peripheral protrusions are in a predetermined reference horizontal plane.
  • a plurality of types of nozzle groups having different distances from the outer peripheral projection to each other, and each nozzle of the nozzle head is placed in the mounting opening of the tip-like container accommodated in the tip-like container accommodation portion.
  • the tip mounting type integrated processing method includes a step of moving the nozzle head to a position where it can be inserted, lowering the nozzle head, and mounting the tip-shaped container on each nozzle of the nozzle head.
  • the lowering of the nozzle head is preferably performed based on at least the structure of the nozzles belonging to each nozzle group and the number of nozzles belonging to each nozzle group. As a result, it can be mounted with an optimum force.
  • a tenth aspect of the invention has a detaching part for detaching the chip-like containers attached to the nozzles all at once, and the detaching part is larger than the maximum outer diameter or the maximum width of the horizontal cross section of the nozzle.
  • a desorption plate provided with holes or gaps having a diameter or width smaller than the maximum outer diameter or maximum width of the chip-like container according to the predetermined arrangement pattern, the plate surface of the desorption plate having the predetermined surface
  • the nozzle is mounted in parallel with a reference horizontal plane and is moved relative to the nozzle along the axial direction of the nozzle so that the axis of each nozzle passes through the hole or the gap.
  • a chip mounting type integration processing method including a step of detaching the chip container.
  • the sixth invention two or more nozzles belonging to a plurality of nozzle groups are provided in the nozzle head, thereby providing a space between all the nozzles and all the chip-like containers.
  • a large force corresponding to the sum of the resistance forces based on the static friction force is dispersed to the number of times corresponding to the number of groups of the nozzle group, whereby the nozzle Reduce the maximum force applied to the head, reduce the force applied to each outer protrusion, prevent wear of each outer protrusion, and reduce operating costs
  • the life of the apparatus can be extended, and thereby product management can be facilitated.
  • the outer peripheral lengths of the plurality of outer peripheral protrusions are shortened toward the tip.
  • an inner peripheral wall surface that is in close contact with or in contact with the outer peripheral protrusion of the nozzle is provided.
  • a tapered surface By forming a tapered surface on the rear end side, insertion of the nozzle into the mounting opening of the tip-shaped container can be facilitated. Further, it is preferable that the tip of the nozzle is also tapered.
  • the force to be applied to the nozzle head in advance is obtained based on the structure of the nozzles belonging to the nozzle group and the number of nozzles belonging to each nozzle group. Can apply an appropriate amount of force and polish the outer peripheral protrusion more than necessary. Wear can be prevented and the life of the apparatus can be extended.
  • the fifth invention or the tenth invention by providing a predetermined hole or gap in the detachable plate as the detachable portion, and providing the movably between the nozzle and the detachable plate, With a simple configuration, it is possible to remove and install the nozzle tips attached to the nozzles in a batch and reliably. Further, since the desorption plate is provided in parallel to the predetermined reference horizontal plane, a large force corresponding to the sum of the resistance forces based on the static friction force is applied when the chip-like container is attached to or detached from the nozzle.
  • FIG. 1 schematically shows a chip-mounted integrated processing apparatus 10 according to an embodiment of the present invention.
  • the chip-mounted integrated processing apparatus 10 includes a total of 96 substantially cylindrical nozzles 14a, 14b, 14c, 14d, and a nodule 14a, 14b, 14c, 14d arranged in a matrix of 12 rows by 8 columns.
  • the nozzle heads 12 arranged so as to protrude downward from the nozzle array plate 20 having the predetermined reference horizontal surface on the lower surface, and suction / discharge mechanisms (16, 18, 18) for sucking and discharging gas through the nozzles 22, 24, 26, 28, 30) and a moving means (34, 36, 38, 40) for moving the nozzle head 12 relative to the chip housing portion.
  • a dispensing chip as a chip-shaped container mounted on the nozzles 14a, 14b, 14c, 14d, and the dispensing chip are arranged in the array. It has a chip accommodating part as a chip-like container accommodating part to be described later, which is arranged according to the pattern and accommodates the nozzles 14a, 14b, 14c, 14d in a state where it can be mounted.
  • the suction / discharge mechanism (16, 18, 22, 24, 26, 28, 30) (The above-mentioned nose and nore head 12 ⁇ are arranged in a matrix of 12 rows by 8 columns of the array pattern.
  • 96 cylinders 16 arranged in a pattern and communicating with the above-mentioned Noznoles 14a, 14b, 14c, 14d, respectively, and the cylinders 16 96 rods 18 for driving a plunger (not shown) slidably inserted therein, a rod driving plate 22 for connecting the 96 rods, and the rod driving plate 22
  • the rod driving plate 22 is connected to the two columnar actuators 24 for driving the rods in the vertical direction (Z-axis direction) at the same time, and the actuators 24, and the actuators 24 are moved in the vertical direction.
  • the nozzle head support 32 supports the suction / discharge motor 30 and the nozzle array plate 20 and supports the actuator 24 so as to be movable up and down.
  • the moving means (34, 36, 38, 40) includes a ball screw that rotationally drives the nozzle head 12 in the X-axis direction (row direction of the array pattern) by an X-axis motor (not shown).
  • a Y-axis drive mechanism having a ball screw that is rotationally driven by an X-axis drive mechanism (not shown) and a Y-axis motor (not shown), and driving in the Y-axis direction (column direction of the array pattern) (Not shown), a frame 33 that is movable in the X-axis direction and the Y-axis direction, and a ball screw 34 that is rotatably supported by the frame 33 and extends in the vertical direction (Z-axis direction); A nut that engages with the ball screw 34 and is connected to the nozzle head support 32 and translates the nozzle head support 32 and thus the nozzle head 12 upward and downward by rotation of the ball screw 34.
  • the apparatus includes a control unit for controlling the moving means, the suction / discharge mechanism, and the like.
  • the control unit includes, for example, an information processing device including a CPU and a memory, a data input device such as a mouse and a keyboard, a display device such as a liquid crystal panel, a data output device such as a printer, a communication means, or a CD, DVD, flexible disk, etc.
  • the external memory drive device includes, for example, an information processing device including a CPU and a memory, a data input device such as a mouse and a keyboard, a display device such as a liquid crystal panel, a data output device such as a printer, a communication means, or a CD, DVD, flexible disk, etc.
  • the external memory drive device includes, for example, an information processing device including a CPU and a memory, a data input device such as a mouse and a keyboard, a display device such as a liquid crystal panel, a data output device such as a printer, a communication means, or a
  • FIG. 2 is a plan view of the nozzle array plate 20 provided in the nozzle head 12 as viewed from below.
  • the lower surface of the nozzle array plate 20 corresponds to the predetermined reference horizontal plane.
  • Each of the 96 nozzles 14a, 14b, 14c, and 14d employs 24 nozzles each belonging to four types of nozzle groups, which will be described later, and has an array pattern of 12 rows x 8 columns.
  • the first nozzle group has an order determined according to the distance or length between the outer peripheral protrusion on the tip side, which will be described later, and the predetermined reference horizontal plane, that is, the length is alternately increased or decreased.
  • the number of nozzles belonging to each nozzle group is 24.
  • the resistance force that the entire nozzle head 12 receives from the entire dispensing tip 46 is dispersed as much as possible.
  • the nozzles 14b belonging to the second nozzle group are arranged in the third row and the seventh row, twelve in the third row and in the third row.
  • No. 14 nozzles belonging to No. 4 are arranged in 12 rows in the 2nd row and 6th row, and 24 nozzles 14d belonging to the No. 4 nozzle group are arranged in 12 rows in the 4th row and 8th row. There is a case.
  • FIG. 3 schematically shows the nozzle head 12 and 96 dispensing tips 46 of the same shape that can be attached to the nozzles 14a, 14b, 14c, and 14d of the nozzle head 12. That is, the chip accommodating portion 42 that is horizontally arranged in a matrix of 12 rows ⁇ 8 columns and accommodated in the state where it can be attached to the nozzles 14a, 14b, 14c, 14d is shown.
  • the tip accommodating portion 42 includes an upper plate 44 having 96 through holes arranged in an array pattern of 12 rows x 8 columns for inserting and supporting the dispensing tips 46, and
  • the four columns 48 having a height capable of supporting the upper plate 44 horizontally at the four corners of the upper plate 44 and supporting the dispensing tips 46 through the through holes of the upper plate 44, and the columns In its four corners, it has a lower plate 50 for supporting it upright.
  • the size of the through hole is such that the main body of the dispensing tip 46 (a thick tube portion 62, a thin tube portion 66 and a transition portion 68 described later) can be inserted, but a plurality of protrusions provided at the upper end are formed.
  • the formed ridge forming portion 47 is formed in a size that cannot be inserted.
  • a microplate (not shown) in which various liquids are accommodated or wells that can be accommodated are arranged in the arrangement pattern! / FIG. 4 shows a state in which the nozzle head 12 and the chip accommodating portion 42 are looked up from below.
  • Each of 96 noses and nores 14a, 14b, 14c, and 14d is passed through 96 nozzle support members 51 having the same shape from the reference horizontal plane that is the lower surface of the nozzle arrangement plate 20 of the nose and nore head 12. It is provided so as to protrude vertically downward.
  • FIG. 5 shows a state in which the dispensing tip 46 is attached to the nozzles 14 a, 14 b, 14 c, 14 d provided on the nozzle head 12.
  • FIG. 6 shows a state where the dispensing tip 46 is attached to one kind of nozzle 14 a provided in the nozzle head 12.
  • the nozzle 14a protrudes from the lower surface of the nozzle array plate 20 of the nozzle head 12, and the lower side of the nozzle support member 51 in which a cylindrical channel 51a communicating with the nozzle 14a is provided in the center. It is provided in connection with.
  • the nozzle 14a protrudes at a certain height outwardly with respect to the main body 56a along a cylindrical main body 56a and an annular outer peripheral band that is closed so as to surround the axis of the nozzle, and mutually in the axial direction. It has a rear end side outer peripheral protrusion 52a and a front end side outer peripheral protrusion 54a which are provided apart from each other.
  • the width of the outermost edge of the outer peripheral projection is narrower than the width of the outer peripheral band on the outer peripheral surface.
  • the front end side outer peripheral protrusion 54a is formed to have a shorter outer peripheral length than the rear end side outer peripheral protrusion 52a.
  • the tip 58a of the nozzle 14a has a taper surface tapered toward the tip 58a of the nozzle 14a on the lower side of the outer peripheral protrusion 54a.
  • the dispensing tip 46 attached to the nozzle 14a is formed in a substantially cylindrical shape as a whole, is provided at the rear end thereof, and is attached to or attachable to the nozzle 14a (or 14b, 14c, 14d).
  • An opening for mounting 60, a port 64 provided at the tip, and capable of entering and exiting fluid by suction and discharge of the gas by the nozzle 14a (or 14b, 14c, 14d), and the opening 60 for mounting A thick tube portion 62 provided on the upper side, the mouth portion 64 is provided at the lower end, and is formed to be narrower than the thick tube portion 62, and has a substantially tapered thin tube portion 66, and the thick tube portion 62 and the thin tube portion. 66 and a funnel-shaped transition portion 68 provided between them.
  • the mounting opening 60 is provided at a distal end inner circumferential cylindrical wall surface 72 that is in close contact with the distal end outer circumferential projection 54a, and spaced from the distal end inner circumferential cylindrical wall surface 72.
  • a tapered surface 76 that is tapered downward is formed between the cylindrical wall surface 70 and a tapered surface 74 that is tapered downward is also formed on the rear end side of the inner peripheral cylindrical wall surface 70 of the rear end side.
  • there is a ridge forming portion 47 provided with a plurality of ridges along the axial direction.
  • FIG. 7 shows a case where the same type of dispensing tip 46 is attached to each of the nozzles 14 &, 1413, 14 and 14 (1) belonging to the four types of nozzles of the nozzle head 12.
  • FIG. 7 shows a nozzle 14a belonging to the first nozzle group to which the dispensing tip 46 is attached, a nozzle 14b belonging to the second nozzle group, a nozzle 14c belonging to the third nozzle group, and a fourth nozzle. Belong to a group
  • the distances from the lower surface of the nozzle array plate 20 to the rear end side outer peripheral projections 52a, 52b, 52c, 52d as the predetermined reference horizontal plane are U, U, U, U, respectively, and from the lower surface to the tip side. If the distances to the outer protrusions 54a, 54b, 54c, 54d are L, L, L, and L, respectively, abed
  • front end side outer peripheral protrusions 54a, 54b, 54c, 54d are not only the front end side inner peripheral cylindrical wall surface 72, but the rear end side outer peripheral protrusions 52a, 52b, 52c, 52d are the rear end side.
  • FIG. 1, FIG. 2, FIG. 3, and FIG. It is moved upward using an X-axis drive mechanism (not shown) and a Y-axis drive mechanism (not shown) of the moving means.
  • the Z-axis motor 40 and the ball screw 43 are driven to lower the nozzle head support 32 and the nozzle head 12 all at once toward the chip housing portion 42.
  • the outer peripheral protrusions of any one of the nozzles 14a, 14b, 14c, 14d come into contact with any one of the inner peripheral cylindrical wall surfaces of the dispensing tip 46, these nozzles, and therefore the nozzle head 12 is
  • the resistance force received from the dispensing tip 46 is 0 or very small.
  • the nozzles provided in the nozzle head 12 are formed of four types of nozzle groups having different distances from the reference horizontal plane to the outer peripheral protrusion, and therefore, among the nozzles 14a, 14b, 14c, and 14d,
  • the outer peripheral protrusion 54a on the tip side of the nozzle 14a belonging to the first nozzle group having the largest distance L from the reference horizontal plane first comes into contact with the inner cylindrical wall surface 72 on the tip side of the dispensing tip 46. Descent to position. Resistance based on the generation of collision and drag due to the narrowing of the distal inner cylindrical wall 72 and the static frictional force between the objects in close contact between the distal outer circumferential protrusion 54a and the distal inner cylindrical wall 72 Nozzle 14a receives force ⁇ .
  • the rear end side inner peripheral cylindrical wall surface 70 belongs to the fourth nozzle group having the largest U and the distance U from the reference horizontal plane among the nozzles 14a, 14b, 14c, 14d.
  • the resistance force due to friction is small because, for example, the state between the rear end side outer peripheral projection and the rear end side inner peripheral cylindrical shape is not a close state but a contact state, and the drag is smaller than that of the latter. Because of its small size, the friction coefficient is a combination of materials that come into contact with the presence of lubricant. It may be smaller than the latter depending on the absence or quality, surface smoothness, cleanliness, material, etc.
  • the nozzle head 12 receives a resistance force R due to a dynamic friction force smaller than the resistance force with respect to the nozzle 14b.
  • This resistance force R is related to the product 24 mN of the dynamic friction coefficient m and the normal resistance N acting between the outer peripheral projection and the inner peripheral cylindrical wall surface. Therefore, as described above, the resistance force based on the dynamic friction coefficient is smaller than the resistance force based on the static friction coefficient.
  • p is the resistance force that one nozzle 14d receives based on the collision or static friction coefficient) d d
  • the nozzle head 12 receives the force.
  • 96 dispensing tips 46 are attached to the respective nozzles 14a, 14b, 14c, 14d of the nozzle head 12.
  • the force p received by one nozzle is related to the resistance force based on the collision or static friction coefficient, that is, mN, and
  • FIGS. 9 to 11 schematically show chip-mounted integrated processing apparatuses 100 and 110 according to the second and third embodiments of the present invention.
  • the attachment / detachment portion 11 of the chip mounting integrated processing apparatus 100 according to the second embodiment shown in FIG. 9 is supported by the nozzle head 12 and a total of 96 front parts provided on the nozzle head 12 are provided.
  • the detaching plate 15 provided so as to be relatively movable with respect to the nozzles 14a, 14b, 14c, 14d is a plate surface fi with respect to the nozzle array plate 20 corresponding to a predetermined reference horizontal plane of the nozzle head 12. In the lower part J of the IJ plate 20 to each noss, nore 14a, 14b, 14c, 14d It is located above the dispensing tip 46 to be installed. Therefore, the detaching plate 15 is moved to this position before the dispensing tip 46 is attached.
  • the detaching plate 15 of the detachable portion 11 is more than the rear end side outer peripheral protrusions 52a, 52b, 52c, 52d corresponding to the maximum outer diameter of the horizontal cross section of the nozzles 14a, 14b, 14c, 14d. Is it larger than the ridge formation of the opening 60 for mounting corresponding to the maximum width of the dispensing tip 46 as the tip-shaped container? L13 force Noss, Noles 14a, 14b, 14c, 14d are arranged in the nozzle arrangement plate 20, that is, a matrix of 12 rows x 8 columns, and formed in the nozzle arrangement plate 20. It is formed with the same row spacing and column spacing.
  • the detaching plate 15 is provided in parallel to the nozzle array plate 20 corresponding to the predetermined reference horizontal plane, and the axes of the nozzles 14a, 14b, 14c, 14d pass through the holes 13 by a moving mechanism (not shown). Thus, it is provided so as to be movable with respect to the nozzle along the axial direction of the nozzle.
  • the detachable portion 111 of the chip mounting type integrated processing apparatus 110 according to the third embodiment shown in FIGS. 10 and 11 is fixedly provided on the stage on which the chip accommodating portion 42 is provided.
  • a desorption plate 19 formed in a comb shape and a support plate 21 for supporting the desorption plate 19 on a stage are provided.
  • the plate surface of the detachable plate 19 is provided parallel to the nozzle array plate 20 corresponding to a predetermined reference horizontal surface of the nozzle head 12.
  • Reference numeral 17 denotes an opening of a tip accommodating portion that is embedded in the stage and accommodates the dispensing tip 46.
  • the detaching plate 19 of the detachable portion 111 is more than the rear end side outer peripheral protrusions 52a, 52b, 52c, 52d corresponding to the maximum outer diameter of the horizontal section of the nozzles 14a, 14b, 14c, 14d.
  • a plurality of gaps (12 in this example) having a width smaller than that of the protrusion forming portion 47 of the mounting opening 60 corresponding to the maximum width of the dispensing tip 46 as the tip-shaped container is large.
  • a plurality of comb-tooth members 23 (11 in this example) sandwiched between one side and the other of the detachable plate 19 are arranged in the nozzle array plate 20, that is, 12 rows.
  • the detaching plate 15 is provided in parallel to the nozzle array plate 20 corresponding to the predetermined reference horizontal plane.
  • the nozzles 14a, 14b, 14c and 14d pass through the holes 13 by a moving mechanism (not shown).
  • the nozzle head 12 is moved by the moving means, and the nozzle nose is moved. 14a, 14b, 14c, and 14d forces are inserted by moving in the row direction into the gaps of the detachable plate 19 above the mounted dispensing tips 46.
  • the moving means applies a force along the direction in which the dispensing tip 46 is detached from the nozzle, that is, the nozzle head 12 is directed upward, along the axial direction of the nozzle or dispensing tip. In addition, it is configured to chop off.
  • the nozzle mainly has two outer peripheral protrusions, and is in contact between the upper outer peripheral protrusion and the upper inner peripheral wall surface, and the lower outer peripheral protrusion and the lower outer peripheral protrusion are in contact with each other.
  • the case of close contact with the inner peripheral wall surface has been described.
  • the present invention is not limited to this case. For example, when one or three outer peripheral protrusions are provided in one nozzle, or the upper outer peripheral protrusion is provided. It may be a case where it is in close contact with the upper inner wall surface.
  • the tip-shaped container is not limited to the above-described one, and may have a step at the transition part or a step other than the transition part. Further, instead of a dispensing tip, a particulate, block, elongated, or wound carrier may be enclosed in a tip-like container.
  • the arrangement pattern is not limited to the case of 12 rows ⁇ 8 columns.
  • a short tube having a smaller diameter such as stainless steel may be further fitted into the mouth portion of the thin tube of the chip-shaped container to increase the dispensing accuracy.
  • the chip-mounted integrated processing apparatus, chip-shaped container, and chip-mounted processing method according to the present invention include fields requiring processing of various solutions, such as industrial fields, foods, agricultural products, and fishery processing. In the fields of agriculture, pharmaceuticals, hygiene, insurance, immunity, disease, genetics, etc., and chemical and biology fields.
  • the present invention is particularly effective when a series of processes using a large number of reagents and substances are continuously performed in a predetermined order.
  • FIG. 1 is a perspective view showing a chip mounting type integrated processing apparatus according to a first embodiment of the present invention.
  • FIG. 2 is a surface view showing a nozzle arrangement pattern of the nozzle head according to the first embodiment of the present invention.
  • FIG. 3 is a perspective view schematically showing a nozzle head and a chip accommodating portion according to the first embodiment of the present invention.
  • FIG. 4 is a perspective view schematically showing a nozzle head and a chip accommodating portion according to the first embodiment of the present invention.
  • FIG. 5 is a perspective view showing a chip mounting type integrated processing apparatus in which a dispensing chip according to the first embodiment of the present invention is mounted on a nozzle.
  • FIG. 6 is a partially cutaway view showing a dispensing tip according to the first embodiment of the present invention.
  • FIG. 7 is a partially cutaway view when a dispensing tip is attached to four types of nozzles belonging to each nozzle group according to the first embodiment of the present invention.
  • FIG. 8 shows the amount of insertion and resistance of the nozzle into the dispensing tip according to the first embodiment of the present invention.
  • FIG. 9 shows the chip-mounted integrated processing device according to the second embodiment of the present invention.
  • Nozzle head It is a perspective view which shows typically the provided removal
  • FIG. 10 is a perspective view schematically showing an attaching / detaching portion provided on a stage of a chip mounting type integrated processing apparatus according to a third embodiment of the present invention.
  • FIG. 11 is a perspective view schematically showing an attaching / detaching portion provided on a stage of a chip mounting integrated processing apparatus according to a third embodiment of the present invention.
  • Chip container (chip-shaped container container)

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un appareil de traitement d'intégration de type à montage de puce qui réalise sans à-coups une opération de montage par lots pour un montage simultané d'une pluralité de puces sur une pluralité de buses. L'invention concerne également un contenant en forme de puce et un procédé de traitement d'intégration de type à montage de puce. L'appareil de traitement d'intégration de type à montage de puce est doté de buses, dont chacune possède une ou plusieurs sections périphériques externes faisant saillie vers l'extérieur ; une tête de buse dans laquelle deux ou plusieurs buses sont disposées dans un motif d'agencement prescrit ; un mécanisme d'aspiration/de décharge ; deux ou plusieurs contenants en forme de puce analogues à des tubes ayant une section d'ouverture de montage et un orifice disposé à l'extrémité avant pour permettre à un fluide de s'écouler à l'intérieur et à l'extérieur ; une section de stockage de contenant en forme de puce dans laquelle les contenants en forme de puce sont stockés ou doivent être stockés dans un état dans lequel les contenants en forme de puce peuvent être montés sur les buses dans le motif d'agencement prescrit ; et des moyens de déplacement pour déplacer relativement les contenants entre les têtes de buse et la section de stockage de contenant en forme de puce. Les buses disposées sur la tête de buse sont configurées de telle sorte que la section périphérique externe faisant saillie disposée sur chaque buse par montage du contenant en forme de puce est amenée en contact ou en contact étroit avec la surface de paroi périphérique interne de la section d'ouverture de montage et que des distances de la surface horizontale de référence prescrite à au moins une section périphérique externe faisant saillie sont différentes les unes des autres.
PCT/JP2007/064596 2006-07-25 2007-07-25 Appareil de traitement d'intégration de type à montage de puce, contenant en forme de puce et procédé de traitement d'intégration de type à montage de puce WO2008013205A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008526792A JP4944888B2 (ja) 2006-07-25 2007-07-25 チップ装着式集積処理装置、チップ状容器、およびチップ装着式集積処理方法

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JP2006-202315 2006-07-25
JP2006202315 2006-07-25

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WO2008013205A1 true WO2008013205A1 (fr) 2008-01-31

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109791163A (zh) * 2016-08-22 2019-05-21 环球生物研究株式会社 分注用缸体、使用了分注用缸体的分注装置及分注处理方法
EP3974841A1 (fr) * 2020-09-25 2022-03-30 Tecan Trading AG Dispositif de pipetage multicanaux
WO2022230448A1 (fr) * 2021-04-28 2022-11-03 ユニバーサル・バイオ・リサーチ株式会社 Dispositif de distribution et système de traitement/mesure d'analyte

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07501006A (ja) * 1991-11-08 1995-02-02 アボツト・ラボラトリーズ 自己位置合せ機能と自己密封機能を備えるピペット・ティップ
JPH08254539A (ja) * 1995-03-15 1996-10-01 Sanyo Electric Co Ltd 分注装置
JP2000097950A (ja) * 1998-09-21 2000-04-07 Matsushita Electric Ind Co Ltd 分注装置
JP2001324509A (ja) * 2000-05-16 2001-11-22 Aloka Co Ltd ノズル装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07501006A (ja) * 1991-11-08 1995-02-02 アボツト・ラボラトリーズ 自己位置合せ機能と自己密封機能を備えるピペット・ティップ
JPH08254539A (ja) * 1995-03-15 1996-10-01 Sanyo Electric Co Ltd 分注装置
JP2000097950A (ja) * 1998-09-21 2000-04-07 Matsushita Electric Ind Co Ltd 分注装置
JP2001324509A (ja) * 2000-05-16 2001-11-22 Aloka Co Ltd ノズル装置

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109791163A (zh) * 2016-08-22 2019-05-21 环球生物研究株式会社 分注用缸体、使用了分注用缸体的分注装置及分注处理方法
CN109791163B (zh) * 2016-08-22 2022-10-21 环球生物研究株式会社 分注用缸体、使用了分注用缸体的分注装置及分注处理方法
EP3974841A1 (fr) * 2020-09-25 2022-03-30 Tecan Trading AG Dispositif de pipetage multicanaux
WO2022230448A1 (fr) * 2021-04-28 2022-11-03 ユニバーサル・バイオ・リサーチ株式会社 Dispositif de distribution et système de traitement/mesure d'analyte

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JP4944888B2 (ja) 2012-06-06

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